MD07G1303000.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007447
Physical Location & Seq
Reverse (-)
36099995 .. 36101174
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1303000.v1.1.491

Sequence Viewer

Length: 738 bp
ATGCAGCAAAGAGTTTTGGATTACGTTTTGGTGCCGGCAGGGCTGCTGGTAATGGTGGCATACCACCTATGGCTTCTCTATCGAATCCTGGCGCAGCCCAACAGCACCGTCATCGGAATCAATTCCATCAACCGCCGCTTCTGGGTTCACGCAATGATGGAGGAAGCTCCAAAGCATGGTGTTCTTGCAGTGCAAACATTGAGAAACAACATAATGGCGTCGACCCTTTTGGCCTCGACCGCCATTACGCTCTGCTCTCTCATTGCCCTCTTGATGACCAGCGGCACCAAATACAGAGCAGCTTTATTTGTCTTTGGGGACCGAAGCGAGCTTGCATTTTCGATCAAGTTCTTCGCCATTTTGGTGTGTTTCCTGGTGGCTTTCCTCTTCAATGTGCAGTCCATAAGGTACTACAGCCACGCCAGCATTCTCATCAACGCACCGTGCAAGAAGATGTCGCCCCACCATCAGCACCACTACCTCACTACAGACTACGTTGCCACAACCGTGAACCGAGGCAGCTATTTCTGGTCCTTGGGACTCCGGGCGTTCTACTTCTCGTTCCCTTTGTTTCTATGGATTTTCGGGCCAATTCCGATGTTCGTGTCCTGCTTTGTTTTGGTCATCATGCTCTACTTCCTAGATGTCACCTTTCAGTTTGGTTGGGAGGTTGGGGTTGCTGACAACGATGATCACATCCTCATTAAGGACGATGAGGAACAAAGGTTGTCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

246

Amino Acids

28.07

Weight (kDa)

8.34

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 9 - 218 6e-76 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 31, 284
AccB7I CCANNNNNTGG 1 cut(s) 176
AccI GTMKAC 1 cut(s) 221
AciI CCGC 4 cut(s) 133, 136, 240, 282
AcyI GRCGYC 1 cut(s) 218
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 3 cut(s) 142, 176, 706
AgsI TTSAA 1 cut(s) 391
AjnI CCWGG 2 cut(s) 87, 372
AleI CACNNNNGTG 1 cut(s) 506
AluBI AGCT 4 cut(s) 167, 302, 331, 522
AluI AGCT 4 cut(s) 167, 302, 331, 522
AoxI GGCC 2 cut(s) 231, 587
ApeKI GCWGC 5 cut(s) 4, 43, 94, 299, 519
Asp700I GAANNNNTTC 1 cut(s) 121
AspLEI GCGC 1 cut(s) 94
AspS9I GGNCC 3 cut(s) 319, 531, 587
AsuC2I CCSGG 1 cut(s) 545
AsuHPI GGTGA 1 cut(s) 640
AvaII GGWCC 2 cut(s) 319, 531
BanI GGYRCC 2 cut(s) 31, 284
BbvI GCAGC 5 cut(s) 16, 30, 106, 311, 531
BccI CCATC 3 cut(s) 134, 151, 474
BcgI CGANNNNNNTGC 2 cut(s) 94, 128
BciT130I CCWGG 2 cut(s) 89, 374
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 1 cut(s) 545
BfaI CTAG 2 cut(s) 641, 736
BfmI CTRYAG 2 cut(s) 412, 486
BglI GCCNNNNNGGC 1 cut(s) 40
BisI GCNGC 7 cut(s) 5, 44, 95, 136, 283, 300, 520
BlsI GCNGC 7 cut(s) 6, 45, 96, 137, 284, 301, 521
Bme1390I CCNGG 3 cut(s) 89, 374, 545
Bme18I GGWCC 2 cut(s) 319, 531
BmgT120I GGNCC 3 cut(s) 319, 531, 587
BmiI GGNNCC 3 cut(s) 33, 286, 320
BmrFI CCNGG 3 cut(s) 89, 374, 545
BpuMI CCSGG 1 cut(s) 545
BsaHI GRCGYC 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 514, 534
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 176, 706
Bse118I RCCGGY 1 cut(s) 34
Bse3DI GCAATG 2 cut(s) 159, 261
BseBI CCWGG 2 cut(s) 89, 374
BseDI CCNNGG 2 cut(s) 514, 534
BseGI GGATG 1 cut(s) 696
BseLI CCNNNNNNNGG 3 cut(s) 142, 176, 706
BseMI GCAATG 2 cut(s) 159, 261
BseXI GCAGC 5 cut(s) 16, 30, 106, 311, 531
BsgI GTGCAG 1 cut(s) 416
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 2 cut(s) 233, 589
BshNI GGYRCC 2 cut(s) 31, 284
BsiEI CGRYCG 1 cut(s) 240
BsiSI CCGG 2 cut(s) 35, 544
BslFI GGGAC 2 cut(s) 332, 552
BslI CCNNNNNNNGG 3 cut(s) 142, 176, 706
BsmFI GGGAC 2 cut(s) 332, 552
BsmI GAATGC 1 cut(s) 426
BsnI GGCC 2 cut(s) 233, 589
Bsp143I GATC 2 cut(s) 342, 691
BspACI CCGC 4 cut(s) 133, 136, 240, 282
BspANI GGCC 2 cut(s) 233, 589
BspLI GGNNCC 3 cut(s) 33, 286, 320
BspT107I GGYRCC 2 cut(s) 31, 284
BsrDI GCAATG 2 cut(s) 159, 261
BsrFI RCCGGY 1 cut(s) 34
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 514, 534
BssMI GATC 2 cut(s) 342, 691
BssNI GRCGYC 1 cut(s) 218
BssT1I CCWWGG 1 cut(s) 534
Bst2UI CCWGG 2 cut(s) 89, 374
Bst4CI ACNGT 3 cut(s) 109, 444, 508
Bst6I CTCTTC 1 cut(s) 392
BstACI GRCGYC 1 cut(s) 218
BstC8I GCNNGC 4 cut(s) 36, 329, 333, 424
BstENI CCTNNNNNAGG 1 cut(s) 704
BstF5I GGATG 1 cut(s) 696
BstHHI GCGC 1 cut(s) 94
BstKTI GATC 2 cut(s) 345, 694
BstMBI GATC 2 cut(s) 342, 691
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 3 cut(s) 40, 239, 423
BstNI CCWGG 2 cut(s) 89, 374
BstSCI CCNGG 3 cut(s) 87, 372, 543
BstSFI CTRYAG 2 cut(s) 412, 486
BstV1I GCAGC 5 cut(s) 16, 30, 106, 311, 531
BsuRI GGCC 2 cut(s) 233, 589
BtsCI GGATG 1 cut(s) 696
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 4 cut(s) 36, 329, 333, 424
CfoI GCGC 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 319, 531, 587
CseI GACGC 1 cut(s) 207
Csp6I GTAC 1 cut(s) 409
CviAII CATG 2 cut(s) 176, 628
CviQI GTAC 1 cut(s) 409
DpnI GATC 2 cut(s) 344, 693
DpnII GATC 2 cut(s) 342, 691
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
Eco130I CCWWGG 1 cut(s) 534
Eco47I GGWCC 2 cut(s) 319, 531
EcoNI CCTNNNNNAGG 1 cut(s) 704
EcoRII CCWGG 2 cut(s) 87, 372
EcoT14I CCWWGG 1 cut(s) 534
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 2 cut(s) 179, 631
FaiI YATR 7 cut(s) 61, 70, 177, 212, 404, 577, 629
FaqI GGGAC 2 cut(s) 332, 552
FatI CATG 2 cut(s) 175, 627
FbaI TGATCA 1 cut(s) 691
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 7 cut(s) 5, 44, 95, 136, 283, 300, 520
FokI GGATG 1 cut(s) 683
Fsp4HI GCNGC 7 cut(s) 5, 44, 95, 136, 283, 300, 520
FspBI CTAG 2 cut(s) 641, 736
GlaI GCGC 1 cut(s) 93
GluI GCNGC 7 cut(s) 5, 44, 95, 136, 283, 300, 520
HaeIII GGCC 2 cut(s) 233, 589
HapII CCGG 2 cut(s) 35, 544
HgaI GACGC 1 cut(s) 207
HhaI GCGC 1 cut(s) 94
Hin1I GRCGYC 1 cut(s) 218
Hin1II CATG 2 cut(s) 179, 631
Hin6I GCGC 1 cut(s) 92
HinP1I GCGC 1 cut(s) 92
HincII GTYRAC 1 cut(s) 222
HindII GTYRAC 1 cut(s) 222
HinfI GANTC 3 cut(s) 84, 117, 540
HpaII CCGG 2 cut(s) 35, 544
HphI GGTGA 1 cut(s) 640
Hpy166II GTNNAC 3 cut(s) 148, 222, 511
Hpy188I TCNGA 2 cut(s) 116, 597
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 3 cut(s) 148, 222, 511
Hpy99I CGWCG 1 cut(s) 223
HpyCH4III ACNGT 3 cut(s) 109, 444, 508
HpyCH4IV ACGT 2 cut(s) 24, 495
HpyCH4V TGCA 6 cut(s) 4, 188, 193, 335, 397, 447
HpyF10VI GCNNNNNNNGC 3 cut(s) 40, 239, 423
HpySE526I ACGT 2 cut(s) 24, 495
Hsp92I GRCGYC 1 cut(s) 218
Hsp92II CATG 2 cut(s) 179, 631
HspAI GCGC 1 cut(s) 92
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 2 cut(s) 342, 691
LmnI GCTCC 1 cut(s) 172
Lsp1109I GCAGC 5 cut(s) 16, 30, 106, 311, 531
MaeI CTAG 2 cut(s) 641, 736
MaeII ACGT 2 cut(s) 24, 495
MaeIII GTNAC 1 cut(s) 646
MalI GATC 2 cut(s) 344, 693
MboI GATC 2 cut(s) 342, 691
MboII GAAGA 3 cut(s) 343, 379, 463
MluCI AATT 2 cut(s) 121, 591
MlyI GAGTC 1 cut(s) 534
MnlI CCTC 9 cut(s) 154, 244, 278, 395, 491, 509, 661, 709, 710
MroNI GCCGGC 1 cut(s) 34
MroXI GAANNNNTTC 1 cut(s) 121
MseI TTAA 1 cut(s) 705
MslI CAYNNNNRTG 2 cut(s) 362, 506
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 2 cut(s) 35, 544
MspR9I CCNGG 3 cut(s) 89, 374, 545
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 2 cut(s) 89, 374
MwoI GCNNNNNNNGC 3 cut(s) 40, 239, 423
NaeI GCCGGC 1 cut(s) 36
NciI CCSGG 1 cut(s) 545
NdeII GATC 2 cut(s) 342, 691
NgoMIV GCCGGC 1 cut(s) 34
NlaIII CATG 2 cut(s) 179, 631
NlaIV GGNNCC 3 cut(s) 33, 286, 320
NmuCI GTSAC 1 cut(s) 646
OliI CACNNNNGTG 1 cut(s) 506
PctI GAATGC 1 cut(s) 426
PdiI GCCGGC 1 cut(s) 36
PdmI GAANNNNTTC 1 cut(s) 121
PfeI GAWTC 2 cut(s) 84, 117
PflMI CCANNNNNTGG 1 cut(s) 176
PkrI GCNGC 7 cut(s) 6, 45, 96, 137, 284, 301, 521
PleI GAGTC 1 cut(s) 534
PpsI GAGTC 1 cut(s) 534
Psp6I CCWGG 2 cut(s) 87, 372
PspGI CCWGG 2 cut(s) 87, 372
PspN4I GGNNCC 3 cut(s) 33, 286, 320
PspPI GGNCC 3 cut(s) 319, 531, 587
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
RseI CAYNNNNRTG 2 cut(s) 362, 506
SalI GTCGAC 1 cut(s) 220
SaqAI TTAA 1 cut(s) 705
SatI GCNGC 7 cut(s) 5, 44, 95, 136, 283, 300, 520
Sau3AI GATC 2 cut(s) 342, 691
Sau96I GGNCC 3 cut(s) 319, 531, 587
SchI GAGTC 1 cut(s) 534
ScrFI CCNGG 3 cut(s) 89, 374, 545
SfcI CTRYAG 2 cut(s) 412, 486
SinI GGWCC 2 cut(s) 319, 531
SmiMI CAYNNNNRTG 2 cut(s) 362, 506
Sse9I AATT 2 cut(s) 121, 591
SsiI CCGC 4 cut(s) 133, 136, 240, 282
SspMI CTAG 2 cut(s) 641, 736
StyD4I CCNGG 3 cut(s) 87, 372, 543
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 3 cut(s) 109, 444, 508
TaiI ACGT 2 cut(s) 27, 498
TaqI TCGA 4 cut(s) 82, 221, 236, 341
TaqII GACCGA 1 cut(s) 336
TasI AATT 2 cut(s) 121, 591
TauI GCSGC 2 cut(s) 138, 285
TfiI GAWTC 2 cut(s) 84, 117
Tru1I TTAA 1 cut(s) 705
Tru9I TTAA 1 cut(s) 705
TscAI CASTG 1 cut(s) 195
TseFI GTSAC 1 cut(s) 646
TseI GCWGC 5 cut(s) 4, 43, 94, 299, 519
Tsp45I GTSAC 1 cut(s) 646
TspRI CASTG 1 cut(s) 195
Van91I CCANNNNNTGG 1 cut(s) 176
VpaK11BI GGWCC 2 cut(s) 319, 531
XagI CCTNNNNNAGG 1 cut(s) 704
XmiI GTMKAC 1 cut(s) 221
XmnI GAANNNNTTC 1 cut(s) 121
XspI CTAG 2 cut(s) 641, 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.