Rroxscaffold_4G00277460

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0007447
Physical Location & Seq
Forward (+)
864620 .. 865696
1077 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00277460.1

Sequence Viewer

Length: 732 bp
ATGGAGAGAAAAGATCTTGATTACATTTTGGTCCCAGCAGGCCTCGTAGTCATGGTGGGGTACCATCTCTGGCTCCTGTACCGAATCAAAACCCAACCCAACAGCACTGTCATCGGCATCAATTGCATCAATCGCAGTTTCTGGGTGCGAGCCATGATGGAGGACACGTCCAAAAATGGTGTTCTTGCGGTGCAGACGCTTCGAAACAACATAATGGCATCAACCCTTTTGGCCTCTACGGCGATCATGCTCAGCTCTCTCATTGCTGTGTTGATGAGTAGTGTAAACAAAGATCGAAGTGCGTCGGTTTTCGTTTACGGGAACACAAGCGAGATGGGATACTCCGTCAAATACTTTTCCATTTTGGTCTGTTTCTTGGTGGCTTTCTTGTTCAATGTGCAGTCCATAAGGTACTACAGCCATGCAAGCATTCTGATCAACGCGCCGTTCAAGAAGATGTCTCCGCATCTGAAGCATCACAATTTGACAACAGAGTATGTGGCAGACACTGTGAACAGAGGCAGCTACTTCTGGTCTTTTGGTCTCCGAGCCTTCTACTTTTCGTTCCCTCTGTTCTTGTGGATCTTTGGTCCCATACCGATGTTCTTGTCCTGCTTTGTTTTGGTTGTCATGCTCTATTTCCTTGACGTTACTTTCCAATTTGGGTGGGTTGTTGGGGTTGACGATGAGGAACATAGCATCATCACCAAGGATGAAGAACAAGGGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

243

Amino Acids

27.75

Weight (kDa)

7.73

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 9 - 219 1.5e-77 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 60
AccB1I GGYRCC 1 cut(s) 60
AccII CGCG 1 cut(s) 443
AciI CCGC 2 cut(s) 188, 464
AclWI GGATC 1 cut(s) 590
AcuI CTGAAG 1 cut(s) 491
AfaI GTAC 3 cut(s) 62, 80, 413
AflIII ACRYGT 1 cut(s) 165
AgsI TTSAA 2 cut(s) 394, 451
AjiI CACGTC 1 cut(s) 168
AluBI AGCT 2 cut(s) 255, 525
AluI AGCT 2 cut(s) 255, 525
Alw26I GTCTC 2 cut(s) 465, 548
AlwI GGATC 1 cut(s) 590
AlwNI CAGNNNCTG 2 cut(s) 141, 509
AoxI GGCC 2 cut(s) 40, 231
ApeKI GCWGC 1 cut(s) 522
Asp718I GGTACC 1 cut(s) 60
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 2 cut(s) 31, 590
AsuHPI GGTGA 1 cut(s) 697
AsuII TTCGAA 1 cut(s) 202
AvaII GGWCC 2 cut(s) 31, 590
BanI GGYRCC 1 cut(s) 60
BbvI GCAGC 1 cut(s) 534
BccI CCATC 3 cut(s) 72, 151, 328
BceAI ACGGC 2 cut(s) 255, 430
BcgI CGANNNNNNTGC 4 cut(s) 94, 128, 182, 216
BciVI GTATCC 1 cut(s) 332
BclI TGATCA 1 cut(s) 435
BcoDI GTCTC 2 cut(s) 465, 548
BfmI CTRYAG 1 cut(s) 415
BfuI GTATCC 1 cut(s) 332
BglI GCCNNNNNGGC 1 cut(s) 239
BglII AGATCT 1 cut(s) 13
BisI GCNGC 1 cut(s) 523
BlpI GCTNAGC 1 cut(s) 251
BlsI GCNGC 1 cut(s) 524
Bme18I GGWCC 2 cut(s) 31, 590
BmgBI CACGTC 1 cut(s) 168
BmgT120I GGNCC 2 cut(s) 31, 590
BmiI GGNNCC 4 cut(s) 33, 62, 74, 592
BmsI GCATC 6 cut(s) 126, 135, 227, 475, 484, 708
Bpu1102I GCTNAGC 1 cut(s) 251
Bpu14I TTCGAA 1 cut(s) 202
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 1 cut(s) 708
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bse3DI GCAATG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 708
BseGI GGATG 1 cut(s) 718
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 1 cut(s) 265
BseXI GCAGC 1 cut(s) 534
BseYI CCCAGC 1 cut(s) 34
BsgI GTGCAG 2 cut(s) 212, 419
Bsh1236I CGCG 1 cut(s) 443
BshFI GGCC 2 cut(s) 42, 233
BshNI GGYRCC 1 cut(s) 60
BslFI GGGAC 2 cut(s) 17, 576
BsmAI GTCTC 2 cut(s) 465, 548
BsmFI GGGAC 2 cut(s) 17, 576
BsmI GAATGC 1 cut(s) 429
BsnI GGCC 2 cut(s) 42, 233
Bso31I GGTCTC 1 cut(s) 548
Bsp119I TTCGAA 1 cut(s) 202
Bsp143I GATC 5 cut(s) 13, 243, 292, 435, 582
Bsp1720I GCTNAGC 1 cut(s) 251
BspACI CCGC 2 cut(s) 188, 464
BspANI GGCC 2 cut(s) 42, 233
BspCNI CTCAG 1 cut(s) 264
BspFNI CGCG 1 cut(s) 443
BspLI GGNNCC 4 cut(s) 33, 62, 74, 592
BspPI GGATC 1 cut(s) 590
BspT104I TTCGAA 1 cut(s) 202
BspT107I GGYRCC 1 cut(s) 60
BspTNI GGTCTC 1 cut(s) 548
BsrDI GCAATG 1 cut(s) 261
BssECI CCNNGG 1 cut(s) 708
BssMI GATC 5 cut(s) 13, 243, 292, 435, 582
BssT1I CCWWGG 1 cut(s) 708
Bst4CI ACNGT 2 cut(s) 109, 511
BstAPI GCANNNNNTGC 1 cut(s) 123
BstBI TTCGAA 1 cut(s) 202
BstC8I GCNNGC 3 cut(s) 40, 150, 427
BstDEI CTNAG 1 cut(s) 251
BstF5I GGATG 1 cut(s) 718
BstFNI CGCG 1 cut(s) 443
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 5 cut(s) 16, 246, 295, 438, 585
BstMAI GTCTC 2 cut(s) 465, 548
BstMBI GATC 5 cut(s) 13, 243, 292, 435, 582
BstMWI GCNNNNNNNGC 5 cut(s) 123, 132, 239, 426, 472
BstSFI CTRYAG 1 cut(s) 415
BstUI CGCG 1 cut(s) 443
BstV1I GCAGC 1 cut(s) 534
BstX2I RGATCY 2 cut(s) 13, 582
BstYI RGATCY 2 cut(s) 13, 582
BsuI GTATCC 1 cut(s) 332
BsuRI GGCC 2 cut(s) 42, 233
BtrI CACGTC 1 cut(s) 168
BtsCI GGATG 1 cut(s) 718
BtsIMutI CAGTG 2 cut(s) 105, 507
Cac8I GCNNGC 3 cut(s) 40, 150, 427
CaiI CAGNNNCTG 2 cut(s) 141, 509
CfoI GCGC 1 cut(s) 445
Cfr13I GGNCC 2 cut(s) 31, 590
CseI GACGC 2 cut(s) 205, 291
Csp6I GTAC 3 cut(s) 61, 79, 412
CspCI CAANNNNNGTGG 2 cut(s) 647, 682
CviAII CATG 5 cut(s) 52, 154, 247, 422, 631
CviJI RGCY 9 cut(s) 42, 73, 152, 233, 255, 383, 420, 525, 551
CviKI_1 RGCY 9 cut(s) 42, 73, 152, 233, 255, 383, 420, 525, 551
CviQI GTAC 3 cut(s) 61, 79, 412
DdeI CTNAG 1 cut(s) 251
DpnI GATC 5 cut(s) 15, 245, 294, 437, 584
DpnII GATC 5 cut(s) 13, 243, 292, 435, 582
Eco130I CCWWGG 1 cut(s) 708
Eco147I AGGCCT 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 548
Eco47I GGWCC 2 cut(s) 31, 590
Eco57I CTGAAG 1 cut(s) 491
EcoT14I CCWWGG 1 cut(s) 708
ErhI CCWWGG 1 cut(s) 708
FaeI CATG 5 cut(s) 55, 157, 250, 425, 634
FaqI GGGAC 2 cut(s) 17, 576
FatI CATG 5 cut(s) 51, 153, 246, 421, 630
FbaI TGATCA 1 cut(s) 435
Fnu4HI GCNGC 1 cut(s) 523
FokI GGATG 1 cut(s) 725
Fsp4HI GCNGC 1 cut(s) 523
GlaI GCGC 1 cut(s) 444
GluI GCNGC 1 cut(s) 523
GsaI CCCAGC 1 cut(s) 38
HaeIII GGCC 2 cut(s) 42, 233
HgaI GACGC 2 cut(s) 205, 291
HhaI GCGC 1 cut(s) 445
Hin1II CATG 5 cut(s) 55, 157, 250, 425, 634
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HinfI GANTC 1 cut(s) 84
HphI GGTGA 1 cut(s) 697
Hpy166II GTNNAC 4 cut(s) 286, 316, 514, 682
Hpy188I TCNGA 3 cut(s) 435, 471, 548
Hpy188III TCNNGA 2 cut(s) 17, 451
Hpy8I GTNNAC 4 cut(s) 286, 316, 514, 682
Hpy99I CGWCG 1 cut(s) 307
HpyAV CCTTC 1 cut(s) 562
HpyCH4III ACNGT 2 cut(s) 109, 511
HpyCH4IV ACGT 2 cut(s) 167, 648
HpyCH4V TGCA 4 cut(s) 126, 193, 400, 425
HpyF10VI GCNNNNNNNGC 5 cut(s) 123, 132, 239, 426, 472
HpyF3I CTNAG 1 cut(s) 251
HpySE526I ACGT 2 cut(s) 167, 648
Hsp92II CATG 5 cut(s) 55, 157, 250, 425, 634
HspAI GCGC 1 cut(s) 443
KpnI GGTACC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 5 cut(s) 13, 243, 292, 435, 582
LmnI GCTCC 1 cut(s) 78
LpnPI CCDG 7 cut(s) 24, 48, 55, 89, 127, 517, 625
Lsp1109I GCAGC 1 cut(s) 534
LweI GCATC 6 cut(s) 126, 135, 227, 475, 484, 708
MaeII ACGT 2 cut(s) 167, 648
MaeIII GTNAC 1 cut(s) 649
MalI GATC 5 cut(s) 15, 245, 294, 437, 584
MboI GATC 5 cut(s) 13, 243, 292, 435, 582
MboII GAAGA 2 cut(s) 466, 728
MfeI CAATTG 1 cut(s) 121
MflI RGATCY 2 cut(s) 13, 582
MluCI AATT 3 cut(s) 121, 481, 659
MnlI CCTC 6 cut(s) 53, 154, 244, 512, 579, 682
MslI CAYNNNNRTG 2 cut(s) 266, 599
MunI CAATTG 1 cut(s) 121
Mva1269I GAATGC 1 cut(s) 429
MvnI CGCG 1 cut(s) 443
MwoI GCNNNNNNNGC 5 cut(s) 123, 132, 239, 426, 472
NdeII GATC 5 cut(s) 13, 243, 292, 435, 582
NlaIII CATG 5 cut(s) 55, 157, 250, 425, 634
NlaIV GGNNCC 4 cut(s) 33, 62, 74, 592
NspV TTCGAA 1 cut(s) 202
PceI AGGCCT 1 cut(s) 42
PctI GAATGC 1 cut(s) 429
PfeI GAWTC 1 cut(s) 84
PkrI GCNGC 1 cut(s) 524
PspFI CCCAGC 1 cut(s) 34
PspN4I GGNNCC 4 cut(s) 33, 62, 74, 592
PspPI GGNCC 2 cut(s) 31, 590
PstNI CAGNNNCTG 2 cut(s) 141, 509
PsuI RGATCY 2 cut(s) 13, 582
RsaI GTAC 3 cut(s) 62, 80, 413
RsaNI GTAC 3 cut(s) 61, 79, 412
RseI CAYNNNNRTG 2 cut(s) 266, 599
SatI GCNGC 1 cut(s) 523
Sau3AI GATC 5 cut(s) 13, 243, 292, 435, 582
Sau96I GGNCC 2 cut(s) 31, 590
SetI ASST 5 cut(s) 170, 257, 413, 527, 651
SfaNI GCATC 6 cut(s) 126, 135, 227, 475, 484, 708
SfcI CTRYAG 1 cut(s) 415
SfuI TTCGAA 1 cut(s) 202
SinI GGWCC 2 cut(s) 31, 590
SmiMI CAYNNNNRTG 2 cut(s) 266, 599
Sse9I AATT 3 cut(s) 121, 481, 659
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 2 cut(s) 188, 464
StuI AGGCCT 1 cut(s) 42
StyI CCWWGG 1 cut(s) 708
TaaI ACNGT 2 cut(s) 109, 511
TaiI ACGT 2 cut(s) 170, 651
TaqI TCGA 2 cut(s) 202, 295
TasI AATT 3 cut(s) 121, 481, 659
TfiI GAWTC 1 cut(s) 84
TscAI CASTG 2 cut(s) 112, 514
TseI GCWGC 1 cut(s) 522
TspDTI ATGAA 1 cut(s) 729
TspGWI ACGGA 1 cut(s) 334
TspRI CASTG 2 cut(s) 112, 514
VpaK11BI GGWCC 2 cut(s) 31, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.