MD13G1115900.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007447
Physical Location & Seq
Forward (+)
8422975 .. 8423518
544 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1115900.v1.1.491

Sequence Viewer

Length: 495 bp
ATGATGGACGATGCTCCAAAGAATGGTATTCTTCCGGTGCAGACGCTTCGAAACAACATAATGGCATCGACCGTTTTGGCCTTGACAGCGATCATGCTCTGCTTTCTCATTGCCCTCTTGATGACCATCAGCACTAGGAGAGCATTTTTCGTTTTCGGAAACCGAAGCGAGTTTGCGTATTCGATCAAGTTCTTCGCCATTTTAGTGTGTTTCTTGGTGGCATTCCTTTTCAACGTGCAGTCCATAAGGTACTACAGCCATGCCAGCATGCTCATCAACGCGTCGTTCAAGAAGATGATGTCGCCGCCTCACCACAAGCAGCAGTACCTCACGCAAGAGTACGTTGCCACCACCGTGAACCGAGGCAGCTATTTCTGGTCCTTGGGACTCCGAGCATTCTACTTCTCGTTCCTTCTGTTTCTGTGGATTTTCGGGCCGATTTCGATGTTCTTGTCGTGCATTGTTTTGGTGATCCTGCTTATTTCACCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

165

Amino Acids

18.86

Weight (kDa)

9.76

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 1 - 161 4.5e-47 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 23
AccII CGCG 1 cut(s) 281
AciI CCGC 1 cut(s) 305
AclWI GGATC 1 cut(s) 466
AfaI GTAC 3 cut(s) 251, 326, 341
AfiI CCNNNNNNNGG 1 cut(s) 23
AflIII ACRYGT 1 cut(s) 279
AgsI TTSAA 2 cut(s) 232, 289
AleI CACNNNNGTG 1 cut(s) 353
AluBI AGCT 1 cut(s) 369
AluI AGCT 1 cut(s) 369
AlwI GGATC 1 cut(s) 466
AoxI GGCC 2 cut(s) 78, 434
ApeKI GCWGC 2 cut(s) 319, 366
AspS9I GGNCC 2 cut(s) 378, 434
AsuHPI GGTGA 3 cut(s) 302, 477, 481
AsuII TTCGAA 1 cut(s) 49
AvaII GGWCC 1 cut(s) 378
BbvI GCAGC 2 cut(s) 331, 378
BccI CCATC 1 cut(s) 134
BcgI CGANNNNNNTGC 2 cut(s) 29, 63
BfaI CTAG 2 cut(s) 135, 493
BfmI CTRYAG 1 cut(s) 253
BisI GCNGC 3 cut(s) 305, 320, 367
BlsI GCNGC 3 cut(s) 306, 321, 368
Bme18I GGWCC 1 cut(s) 378
BmgT120I GGNCC 2 cut(s) 378, 434
BmsI GCATC 1 cut(s) 74
Bpu14I TTCGAA 1 cut(s) 49
BsaBI GATNNNNATC 1 cut(s) 125
BsaJI CCNNGG 2 cut(s) 361, 381
BsaWI WCCGGW 1 cut(s) 34
Bsc4I CCNNNNNNNGG 1 cut(s) 23
Bse3DI GCAATG 1 cut(s) 108
Bse8I GATNNNNATC 1 cut(s) 125
BseDI CCNNGG 2 cut(s) 361, 381
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 1 cut(s) 23
BseMI GCAATG 1 cut(s) 108
BseXI GCAGC 2 cut(s) 331, 378
BsgI GTGCAG 2 cut(s) 59, 257
Bsh1236I CGCG 1 cut(s) 281
Bsh1285I CGRYCG 1 cut(s) 72
BshFI GGCC 2 cut(s) 80, 436
BsiEI CGRYCG 1 cut(s) 72
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 1 cut(s) 399
BslI CCNNNNNNNGG 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 399
BsmI GAATGC 2 cut(s) 221, 395
BsnI GGCC 2 cut(s) 80, 436
Bsp119I TTCGAA 1 cut(s) 49
Bsp143I GATC 3 cut(s) 90, 183, 471
BspACI CCGC 1 cut(s) 305
BspANI GGCC 2 cut(s) 80, 436
BspFNI CGCG 1 cut(s) 281
BspPI GGATC 1 cut(s) 466
BspT104I TTCGAA 1 cut(s) 49
BsrDI GCAATG 1 cut(s) 108
BssECI CCNNGG 2 cut(s) 361, 381
BssMI GATC 3 cut(s) 90, 183, 471
BssT1I CCWWGG 1 cut(s) 381
Bst4CI ACNGT 2 cut(s) 73, 355
BstBI TTCGAA 1 cut(s) 49
BstC8I GCNNGC 2 cut(s) 265, 269
BstFNI CGCG 1 cut(s) 281
BstKTI GATC 3 cut(s) 93, 186, 474
BstMBI GATC 3 cut(s) 90, 183, 471
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 2 cut(s) 86, 264
BstNSI RCATGY 1 cut(s) 271
BstSFI CTRYAG 1 cut(s) 253
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 331, 378
BsuRI GGCC 2 cut(s) 80, 436
Cac8I GCNNGC 2 cut(s) 265, 269
Cfr13I GGNCC 2 cut(s) 378, 434
CseI GACGC 2 cut(s) 52, 270
Csp6I GTAC 3 cut(s) 250, 325, 340
CviAII CATG 3 cut(s) 94, 260, 268
CviJI RGCY 4 cut(s) 80, 258, 369, 436
CviKI_1 RGCY 4 cut(s) 80, 258, 369, 436
CviQI GTAC 3 cut(s) 250, 325, 340
DpnI GATC 3 cut(s) 92, 185, 473
DpnII GATC 3 cut(s) 90, 183, 471
Eco130I CCWWGG 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 381
ErhI CCWWGG 1 cut(s) 381
FaeI CATG 3 cut(s) 97, 263, 271
FaiI YATR 5 cut(s) 59, 95, 245, 261, 269
FaqI GGGAC 1 cut(s) 399
FatI CATG 3 cut(s) 93, 259, 267
Fnu4HI GCNGC 3 cut(s) 305, 320, 367
Fsp4HI GCNGC 3 cut(s) 305, 320, 367
FspBI CTAG 2 cut(s) 135, 493
GluI GCNGC 3 cut(s) 305, 320, 367
HaeIII GGCC 2 cut(s) 80, 436
HapII CCGG 1 cut(s) 35
HgaI GACGC 2 cut(s) 52, 270
Hin1II CATG 3 cut(s) 97, 263, 271
HinfI GANTC 1 cut(s) 387
HpaII CCGG 1 cut(s) 35
HphI GGTGA 3 cut(s) 302, 477, 481
Hpy166II GTNNAC 1 cut(s) 358
Hpy188I TCNGA 2 cut(s) 158, 392
Hpy188III TCNNGA 2 cut(s) 118, 289
Hpy8I GTNNAC 1 cut(s) 358
Hpy99I CGWCG 1 cut(s) 286
HpyAV CCTTC 1 cut(s) 422
HpyCH4III ACNGT 2 cut(s) 73, 355
HpyCH4IV ACGT 2 cut(s) 234, 342
HpyCH4V TGCA 3 cut(s) 40, 238, 459
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 264
HpySE526I ACGT 2 cut(s) 234, 342
Hsp92II CATG 3 cut(s) 97, 263, 271
Kzo9I GATC 3 cut(s) 90, 183, 471
LmnI GCTCC 1 cut(s) 19
LpnPI CCDG 4 cut(s) 48, 277, 361, 488
Lsp1109I GCAGC 2 cut(s) 331, 378
LweI GCATC 1 cut(s) 74
MaeI CTAG 2 cut(s) 135, 493
MaeII ACGT 2 cut(s) 234, 342
MalI GATC 3 cut(s) 92, 185, 473
MboI GATC 3 cut(s) 90, 183, 471
MboII GAAGA 3 cut(s) 23, 184, 304
MluI ACGCGT 1 cut(s) 279
MlyI GAGTC 1 cut(s) 381
MnlI CCTC 4 cut(s) 125, 318, 338, 356
MslI CAYNNNNRTG 2 cut(s) 203, 353
MspI CCGG 1 cut(s) 35
Mva1269I GAATGC 2 cut(s) 221, 395
MvnI CGCG 1 cut(s) 281
MwoI GCNNNNNNNGC 2 cut(s) 86, 264
NdeII GATC 3 cut(s) 90, 183, 471
NlaIII CATG 3 cut(s) 97, 263, 271
NspI RCATGY 1 cut(s) 271
NspV TTCGAA 1 cut(s) 49
OliI CACNNNNGTG 1 cut(s) 353
PaeI GCATGC 1 cut(s) 271
PctI GAATGC 2 cut(s) 221, 395
PflMI CCANNNNNTGG 1 cut(s) 23
PkrI GCNGC 3 cut(s) 306, 321, 368
PleI GAGTC 1 cut(s) 381
PpsI GAGTC 1 cut(s) 381
PspPI GGNCC 2 cut(s) 378, 434
RsaI GTAC 3 cut(s) 251, 326, 341
RsaNI GTAC 3 cut(s) 250, 325, 340
RseI CAYNNNNRTG 2 cut(s) 203, 353
SatI GCNGC 3 cut(s) 305, 320, 367
Sau3AI GATC 3 cut(s) 90, 183, 471
Sau96I GGNCC 2 cut(s) 378, 434
SchI GAGTC 1 cut(s) 381
SetI ASST 6 cut(s) 237, 251, 330, 345, 371, 490
SfaNI GCATC 1 cut(s) 74
SfcI CTRYAG 1 cut(s) 253
SfuI TTCGAA 1 cut(s) 49
SinI GGWCC 1 cut(s) 378
SmiMI CAYNNNNRTG 2 cut(s) 203, 353
SphI GCATGC 1 cut(s) 271
SsiI CCGC 1 cut(s) 305
SspMI CTAG 2 cut(s) 135, 493
StyI CCWWGG 1 cut(s) 381
TaaI ACNGT 2 cut(s) 73, 355
TaiI ACGT 2 cut(s) 237, 345
TaqI TCGA 4 cut(s) 49, 68, 182, 443
TauI GCSGC 1 cut(s) 307
TseI GCWGC 2 cut(s) 319, 366
Van91I CCANNNNNTGG 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 378
XceI RCATGY 1 cut(s) 271
XspI CTAG 2 cut(s) 135, 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.