Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0011148

Physical Location & Seq

Genomic Coordinates Forward (+)

13701290 .. 13703241

Gene Structure 1952 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G35520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 717 bp

ATGGAAGGAAGCAAAAGCGAGGCAGTGTTCGATTCCATGAATCTAAATCCTCAGATTTTCATCAACGAAGCAATCAACTCCGTCGAAGATTATGTCGACCAAGCTTTTGACTTCTATGCCCGTGATGCATCCAAATCTCTCAAAATCAAAGGATCTGATAAACAAAAGTCACAAGCTTTATCAAATGGTATTGCTCGTGTTCGTGGTTTGCTACTATCTGTAATCGACAATCGTTTGAAATTGTGGGAAAGCTATTCTCTTCGATTCTGTTTCGCAGTTCCTGATGGATTTGTGTTACCAAAGAGTGAGGAGTCTTCGTCTGTGCATCAGGATGGGTTATATGATCTAGAATTAGATGCAGAATTGGATTCTTTAAGGGATAAGCTTAATGTGGTGGGAAAGAGGTCTGTTGAGCTTGACTCTGAGCTCCAGGCTTTAGAAAGAAGCTCGGTTTCGAGGGAACGGTCTTTGAGACTCGTTAATGAAGCTTTGGAGCTTTATGATGAGTCTTCTATGGATGAAATATTCAAAGAGATGACGAAAATGGCATCGGAACTTCGTGCAAGCGTGGAGAGGCTGAAAACAAGAAGAATGAAAGCAAGTGAAAGCGCTAAAGTAAAGAGGCTAAAGAACCATGGCAAAGAGTTTTCAGCAATGACTTTTGATGGAAAGCTTGAAGACCTCGAAAAGTTTCAAGCCGAGTTAAGGAAGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000070 GO:0000228 GO:0000278 GO:0000280 GO:0000444 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000818 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006996 GO:0007049 GO:0007059 GO:0008104 GO:0008150 GO:0009987 GO:0016043 GO:0022402 GO:0022607 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0033365 GO:0034501 GO:0034502 GO:0034508 GO:0034613 GO:0034622 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0048285 GO:0051179 GO:0051276 GO:0051382 GO:0051383 GO:0051641 GO:0065003 GO:0065004 GO:0070013 GO:0070727 GO:0070925 GO:0071459 GO:0071824 GO:0071840 GO:0098687 GO:0098813 GO:0140014 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mis12

Protein Analysis

238

Amino Acids

27.13

Weight (kDa)

5.38

Isoelectric Point (pI)

49.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mis12	PF05859	8 - 207	4.4e-41	Mis12 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0011148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35520
fragaria_vesca	FvH4_5g13000
malus_domestica	MD06G1208100.v1.1 MD14G1218500.v1.1
prunus_persica	Prupe.5G212600_v2.0.a1 Prupe.5G212600_v2.0.a1
pyrus_communis	pycom06g18590 pycom14g18110
rosa_chinensis	RchiOBHm_Chr7g0184161
rosa_laevigata	RLG00000004998
rosa_multiflora	Rmu_co8235265.1_g000001 Rmu_sc0012221.1_g000004
rosa_roxburghii	Rroxscaffold_3G00269840
rosa_rugosa	Rorug06G0463400 Rorug06G0463500
rosa_samantha	Rh7AG082400 Rh7BG064800 Rh7CG065200 Rh7DG064900
rosa_wichuraiana	Rw7G005310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	96
AclWI	GGATC	1 cut(s)	160
AfeI	AGCGCT	1 cut(s)	610
AfiI	CCNNNNNNNGG	1 cut(s)	705
AgsI	TTSAA	4 cut(s)	238, 529, 677, 695
AjnI	CCWGG	1 cut(s)	429
Alw21I	GWGCWC	1 cut(s)	429
Alw26I	GTCTC	1 cut(s)	466
AlwI	GGATC	1 cut(s)	160
AlwNI	CAGNNNCTG	1 cut(s)	281
Aor51HI	AGCGCT	1 cut(s)	610
ArsI	GACNNNNNNTTYG	4 cut(s)	78, 89, 110, 121
Asp700I	GAANNNNTTC	1 cut(s)	690
AspLEI	GCGC	1 cut(s)	611
BanII	GRGCYC	1 cut(s)	429
BauI	CACGAG	1 cut(s)	195
BbsI	GAAGAC	3 cut(s)	306, 501, 684
Bbv12I	GWGCWC	1 cut(s)	429
BccI	CCATC	3 cut(s)	278, 326, 659
BciT130I	CCWGG	1 cut(s)	431
BcoDI	GTCTC	1 cut(s)	466
BfaI	CTAG	1 cut(s)	347
BfoI	RGCGCY	1 cut(s)	612
Bme1390I	CCNGG	1 cut(s)	431
BmrFI	CCNGG	1 cut(s)	431
BmsI	GCATC	5 cut(s)	115, 137, 334, 346, 557
BpiI	GAAGAC	3 cut(s)	306, 501, 684
BplI	GAGNNNNNCTC	2 cut(s)	404, 436
BpmI	CTGGAG	1 cut(s)	413
BsaBI	GATNNNNATC	1 cut(s)	59
BsaJI	CCNNGG	1 cut(s)	634
BsaXI	ACNNNNNCTCC	2 cut(s)	302, 332
Bsc4I	CCNNNNNNNGG	1 cut(s)	705
Bse3DI	GCAATG	1 cut(s)	660
Bse8I	GATNNNNATC	1 cut(s)	59
BseBI	CCWGG	1 cut(s)	431
BseDI	CCNNGG	1 cut(s)	634
BseGI	GGATG	3 cut(s)	128, 337, 523
BseJI	GATNNNNATC	1 cut(s)	59
BseLI	CCNNNNNNNGG	1 cut(s)	705
BseMI	GCAATG	1 cut(s)	660
BseMII	CTCAG	2 cut(s)	65, 414
BseRI	GAGGAG	1 cut(s)	323
BsiHKAI	GWGCWC	1 cut(s)	429
BslI	CCNNNNNNNGG	1 cut(s)	705
BsmAI	GTCTC	1 cut(s)	466
Bsp1286I	GDGCHC	1 cut(s)	429
Bsp143I	GATC	2 cut(s)	152, 343
Bsp19I	CCATGG	1 cut(s)	634
BspCNI	CTCAG	2 cut(s)	64, 415
BspPI	GGATC	1 cut(s)	160
BsrDI	GCAATG	1 cut(s)	660
BssECI	CCNNGG	1 cut(s)	634
BssMI	GATC	2 cut(s)	152, 343
BssSI	CACGAG	1 cut(s)	195
BssT1I	CCWWGG	1 cut(s)	634
Bst2BI	CACGAG	1 cut(s)	195
Bst2UI	CCWGG	1 cut(s)	431
Bst4CI	ACNGT	1 cut(s)	465
Bst6I	CTCTTC	1 cut(s)	264
BstC8I	GCNNGC	1 cut(s)	565
BstDEI	CTNAG	2 cut(s)	51, 423
BstDSI	CCRYGG	1 cut(s)	634
BstF5I	GGATG	3 cut(s)	128, 337, 523
BstH2I	RGCGCY	1 cut(s)	612
BstHHI	GCGC	1 cut(s)	611
BstKTI	GATC	2 cut(s)	155, 346
BstMAI	GTCTC	1 cut(s)	466
BstMBI	GATC	2 cut(s)	152, 343
BstMWI	GCNNNNNNNGC	1 cut(s)	125
BstNI	CCWGG	1 cut(s)	431
BstSCI	CCNGG	1 cut(s)	429
BstV2I	GAAGAC	3 cut(s)	306, 501, 684
BstX2I	RGATCY	1 cut(s)	152
BstYI	RGATCY	1 cut(s)	152
BtgI	CCRYGG	1 cut(s)	634
BtsCI	GGATG	3 cut(s)	128, 337, 523
BtsI	GCAGTG	1 cut(s)	30
BtsIMutI	CAGTG	1 cut(s)	30
Cac8I	GCNNGC	1 cut(s)	565
CaiI	CAGNNNCTG	1 cut(s)	281
CfoI	GCGC	1 cut(s)	611
CviAII	CATG	2 cut(s)	37, 635
DdeI	CTNAG	2 cut(s)	51, 423
DpnI	GATC	2 cut(s)	154, 345
DpnII	GATC	2 cut(s)	152, 343
Eam1104I	CTCTTC	1 cut(s)	264
EarI	CTCTTC	1 cut(s)	264
Ecl136II	GAGCTC	1 cut(s)	427
Eco130I	CCWWGG	1 cut(s)	634
Eco24I	GRGCYC	1 cut(s)	429
Eco47III	AGCGCT	1 cut(s)	610
Eco53kI	GAGCTC	1 cut(s)	427
EcoICRI	GAGCTC	1 cut(s)	427
EcoRII	CCWGG	1 cut(s)	429
EcoT14I	CCWWGG	1 cut(s)	634
EcoT22I	ATGCAT	1 cut(s)	130
EcoT38I	GRGCYC	1 cut(s)	429
ErhI	CCWWGG	1 cut(s)	634
FaeI	CATG	2 cut(s)	40, 638
FaiI	YATR	8 cut(s)	38, 93, 117, 340, 342, 501, 515, 636
FatI	CATG	2 cut(s)	36, 634
FblI	GTMKAC	1 cut(s)	96
FokI	GGATG	3 cut(s)	115, 344, 530
FriOI	GRGCYC	1 cut(s)	429
FspBI	CTAG	1 cut(s)	347
GlaI	GCGC	1 cut(s)	610
GsuI	CTGGAG	1 cut(s)	413
HaeII	RGCGCY	1 cut(s)	612
HhaI	GCGC	1 cut(s)	611
Hin1II	CATG	2 cut(s)	40, 638
Hin6I	GCGC	1 cut(s)	609
HinP1I	GCGC	1 cut(s)	609
HincII	GTYRAC	1 cut(s)	97
HindII	GTYRAC	1 cut(s)	97
HindIII	AAGCTT	5 cut(s)	102, 174, 383, 486, 671
HinfI	GANTC	8 cut(s)	32, 40, 264, 311, 368, 419, 474, 506
Hpy166II	GTNNAC	1 cut(s)	97
Hpy188I	TCNGA	4 cut(s)	54, 157, 424, 553
Hpy188III	TCNNGA	3 cut(s)	281, 329, 347
Hpy8I	GTNNAC	1 cut(s)	97
Hpy99I	CGWCG	1 cut(s)	86
HpyCH4III	ACNGT	1 cut(s)	465
HpyCH4V	TGCA	4 cut(s)	128, 325, 359, 563
HpyF10VI	GCNNNNNNNGC	1 cut(s)	125
HpyF3I	CTNAG	2 cut(s)	51, 423
Hsp92II	CATG	2 cut(s)	40, 638
HspAI	GCGC	1 cut(s)	609
Kzo9I	GATC	2 cut(s)	152, 343
LmnI	GCTCC	2 cut(s)	432, 493
LpnPI	CCDG	4 cut(s)	294, 314, 416, 443
LweI	GCATC	5 cut(s)	115, 137, 334, 346, 557
MaeI	CTAG	1 cut(s)	347
MaeIII	GTNAC	2 cut(s)	168, 294
MalI	GATC	2 cut(s)	154, 345
MboI	GATC	2 cut(s)	152, 343
MboII	GAAGA	6 cut(s)	98, 251, 306, 501, 600, 689
MflI	RGATCY	1 cut(s)	152
MhlI	GDGCHC	1 cut(s)	429
MluCI	AATT	3 cut(s)	239, 350, 362
MlyI	GAGTC	4 cut(s)	320, 413, 468, 515
MnlI	CCTC	8 cut(s)	13, 60, 301, 396, 450, 567, 615, 692
Mph1103I	ATGCAT	1 cut(s)	130
MroXI	GAANNNNTTC	1 cut(s)	690
MseI	TTAA	4 cut(s)	374, 387, 480, 704
MslI	CAYNNNNRTG	1 cut(s)	330
MspR9I	CCNGG	1 cut(s)	431
MvaI	CCWGG	1 cut(s)	431
MwoI	GCNNNNNNNGC	1 cut(s)	125
NcoI	CCATGG	1 cut(s)	634
NdeII	GATC	2 cut(s)	152, 343
NlaIII	CATG	2 cut(s)	40, 638
NmuCI	GTSAC	1 cut(s)	168
NsiI	ATGCAT	1 cut(s)	130
PdmI	GAANNNNTTC	1 cut(s)	690
PfeI	GAWTC	4 cut(s)	32, 40, 264, 368
PleI	GAGTC	4 cut(s)	319, 413, 468, 514
PpsI	GAGTC	4 cut(s)	319, 413, 468, 514
Psp124BI	GAGCTC	1 cut(s)	429
Psp6I	CCWGG	1 cut(s)	429
PspGI	CCWGG	1 cut(s)	429
PstNI	CAGNNNCTG	1 cut(s)	281
PsuI	RGATCY	1 cut(s)	152
RseI	CAYNNNNRTG	1 cut(s)	330
SacI	GAGCTC	1 cut(s)	429
SalI	GTCGAC	1 cut(s)	95
SaqAI	TTAA	4 cut(s)	374, 387, 480, 704
Sau3AI	GATC	2 cut(s)	152, 343
SchI	GAGTC	4 cut(s)	320, 413, 468, 515
ScrFI	CCNGG	1 cut(s)	431
SduI	GDGCHC	1 cut(s)	429
SfaNI	GCATC	5 cut(s)	115, 137, 334, 346, 557
SmiMI	CAYNNNNRTG	1 cut(s)	330
Sse9I	AATT	3 cut(s)	239, 350, 362
SspI	AATATT	1 cut(s)	525
SspMI	CTAG	1 cut(s)	347
SstI	GAGCTC	1 cut(s)	429
StyD4I	CCNGG	1 cut(s)	429
StyI	CCWWGG	1 cut(s)	634
TaaI	ACNGT	1 cut(s)	465
TaqI	TCGA	7 cut(s)	30, 84, 96, 225, 262, 455, 684
TasI	AATT	3 cut(s)	239, 350, 362
TfiI	GAWTC	4 cut(s)	32, 40, 264, 368
Tru1I	TTAA	4 cut(s)	374, 387, 480, 704
Tru9I	TTAA	4 cut(s)	374, 387, 480, 704
TscAI	CASTG	1 cut(s)	30
TseFI	GTSAC	1 cut(s)	168
Tsp45I	GTSAC	1 cut(s)	168
TspDTI	ATGAA	5 cut(s)	49, 53, 498, 534, 608
TspGWI	ACGGA	1 cut(s)	70
TspRI	CASTG	1 cut(s)	30
XbaI	TCTAGA	1 cut(s)	346
XmiI	GTMKAC	1 cut(s)	96
XmnI	GAANNNNTTC	1 cut(s)	690
XspI	CTAG	1 cut(s)	347
Zsp2I	ATGCAT	1 cut(s)	130

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.