Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0011148

Physical Location & Seq

Genomic Coordinates Reverse (-)

30150873 .. 30153687

Gene Structure 2815 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1218500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 735 bp

ATGGAAGGCAGCGAAAGCGAGGCAGCATTCAATTCGCTGAATCTGAATCCGCAGCTGTTCATCAACGACGTCTTAAACATCGCCGATGACTTGGTCGACGACGCCTTCGATCATTTCCGCCAACAAGCATCAACCTCTCTGAAAATTGAGGGCACTGACAGAGCCCAAGATCTATCCAGGGGTGTTGATATCATCCGCAAAAAGGTTCAATCAGATCTTGATAAAAAGCTGGCTGTCTGGGAGCAGTACTGCCTTGACTACGTTTTTCAAGTTCCAGAGGGGTTCGACCCGTCGAAGAATGATGAACCACCTGTCGACGCTTCTTTATGTCAAAATGCAATAGTTGACACAGACCTGGATGCCCAGCTGGATTTGTTGAGGAACGAACTCACTGAGGTTAGTAAGGAGAATGCTGCGCTGAATCGAGAGATCCAAGCATTAGAAGGACAGTCTGTGTCTAGAGATCGTTGTTCTGCGCTTGTCAACGAGACATTGCAACAATATGACCTAAACTCTTACCATGAAAATTTCCAAGAGATGGTGAAAACGGCATCAGCACTTCGGATGAAGGTAGGAAAGCTGCAGACCGAAAAGATGGAAGAATCCAAACGCCGGAAGATTGATTGGATCAACAACGCGCAGAGAGACCCGTCTGTTACGGAAATTCGCAGAGGCTTCTATAGTGCTAAGTTGGAAGATCTTGAAGCATTTATAGCCCATATCAAAAACATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000070 GO:0000228 GO:0000278 GO:0000280 GO:0000444 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000818 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006996 GO:0007049 GO:0007059 GO:0008104 GO:0008150 GO:0009987 GO:0016043 GO:0022402 GO:0022607 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0033365 GO:0034501 GO:0034502 GO:0034508 GO:0034613 GO:0034622 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0048285 GO:0051179 GO:0051276 GO:0051382 GO:0051383 GO:0051641 GO:0065003 GO:0065004 GO:0070013 GO:0070727 GO:0070925 GO:0071459 GO:0071824 GO:0071840 GO:0098687 GO:0098813 GO:0140014 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mis12

Protein Analysis

245

Amino Acids

27.8

Weight (kDa)

4.64

Isoelectric Point (pI)

46.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mis12	PF05859	9 - 209	8.2e-30	Mis12 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35520
fragaria_vesca	FvH4_5g13000
malus_domestica	MD06G1208100.v1.1 MD14G1218500.v1.1
prunus_persica	Prupe.5G212600_v2.0.a1 Prupe.5G212600_v2.0.a1
pyrus_communis	pycom06g18590 pycom14g18110
rosa_chinensis	RchiOBHm_Chr7g0184161
rosa_laevigata	RLG00000004998
rosa_multiflora	Rmu_co8235265.1_g000001 Rmu_sc0012221.1_g000004
rosa_roxburghii	Rroxscaffold_3G00269840
rosa_rugosa	Rorug06G0463400 Rorug06G0463500
rosa_samantha	Rh7AG082400 Rh7BG064800 Rh7CG065200 Rh7DG064900
rosa_wichuraiana	Rw7G005310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	72
AccB7I	CCANNNNNTGG	1 cut(s)	538
AccI	GTMKAC	2 cut(s)	96, 315
AccII	CGCG	1 cut(s)	638
AciI	CCGC	3 cut(s)	50, 118, 196
AclWI	GGATC	2 cut(s)	424, 635
AcsI	RAATTY	2 cut(s)	526, 663
AcyI	GRCGYC	2 cut(s)	69, 102
AfaI	GTAC	1 cut(s)	248
AfiI	CCNNNNNNNGG	3 cut(s)	202, 538, 612
AgsI	TTSAA	4 cut(s)	31, 209, 269, 704
AjnI	CCWGG	2 cut(s)	176, 354
AluBI	AGCT	4 cut(s)	55, 229, 367, 580
AluI	AGCT	4 cut(s)	55, 229, 367, 580
Alw26I	GTCTC	2 cut(s)	482, 639
AlwI	GGATC	2 cut(s)	424, 635
ApeKI	GCWGC	5 cut(s)	9, 23, 52, 413, 580
ApoI	RAATTY	2 cut(s)	526, 663
ArsI	GACNNNNNNTTYG	2 cut(s)	89, 121
AspLEI	GCGC	3 cut(s)	418, 478, 640
AsuHPI	GGTGA	1 cut(s)	553
BaeGI	GKGCMC	1 cut(s)	155
BanII	GRGCYC	1 cut(s)	166
BbvI	GCAGC	5 cut(s)	21, 35, 64, 400, 567
BccI	CCATC	2 cut(s)	532, 589
BceAI	ACGGC	1 cut(s)	564
BcgI	CGANNNNNNTGC	2 cut(s)	15, 49
BciT130I	CCWGG	2 cut(s)	178, 356
BcoDI	GTCTC	2 cut(s)	482, 639
BfaI	CTAG	1 cut(s)	459
BfmI	CTRYAG	2 cut(s)	581, 679
BglII	AGATCT	3 cut(s)	169, 214, 697
BisI	GCNGC	5 cut(s)	10, 24, 53, 414, 581
BlsI	GCNGC	5 cut(s)	11, 25, 54, 415, 582
BmcAI	AGTACT	1 cut(s)	248
Bme1390I	CCNGG	2 cut(s)	178, 356
BmrFI	CCNGG	2 cut(s)	178, 356
BmsI	GCATC	3 cut(s)	137, 349, 560
BsaHI	GRCGYC	2 cut(s)	69, 102
BsaI	GGTCTC	1 cut(s)	639
BsaJI	CCNNGG	1 cut(s)	177
Bsc4I	CCNNNNNNNGG	3 cut(s)	202, 538, 612
Bse3DI	GCAATG	1 cut(s)	491
BseBI	CCWGG	2 cut(s)	178, 356
BseDI	CCNNGG	1 cut(s)	177
BseGI	GGATG	3 cut(s)	192, 364, 570
BseLI	CCNNNNNNNGG	3 cut(s)	202, 538, 612
BseMI	GCAATG	1 cut(s)	491
BseMII	CTCAG	1 cut(s)	384
BseSI	GKGCMC	1 cut(s)	155
BseXI	GCAGC	5 cut(s)	21, 35, 64, 400, 567
BseYI	CCCAGC	1 cut(s)	363
Bsh1236I	CGCG	1 cut(s)	638
BsiSI	CCGG	1 cut(s)	613
BslI	CCNNNNNNNGG	3 cut(s)	202, 538, 612
BsmAI	GTCTC	2 cut(s)	482, 639
BsmI	GAATGC	2 cut(s)	26, 415
Bso31I	GGTCTC	1 cut(s)	639
Bsp1286I	GDGCHC	2 cut(s)	155, 166
Bsp143I	GATC	7 cut(s)	109, 169, 214, 429, 463, 627, 697
BspACI	CCGC	3 cut(s)	50, 118, 196
BspCNI	CTCAG	1 cut(s)	385
BspFNI	CGCG	1 cut(s)	638
BspMAI	CTGCAG	1 cut(s)	585
BspPI	GGATC	2 cut(s)	424, 635
BspTNI	GGTCTC	1 cut(s)	639
BsrDI	GCAATG	1 cut(s)	491
BssECI	CCNNGG	1 cut(s)	177
BssMI	GATC	7 cut(s)	109, 169, 214, 429, 463, 627, 697
BssNI	GRCGYC	2 cut(s)	69, 102
Bst2UI	CCWGG	2 cut(s)	178, 356
Bst4CI	ACNGT	1 cut(s)	450
BstACI	GRCGYC	2 cut(s)	69, 102
BstC8I	GCNNGC	1 cut(s)	231
BstDEI	CTNAG	2 cut(s)	393, 687
BstF5I	GGATG	3 cut(s)	192, 364, 570
BstFNI	CGCG	1 cut(s)	638
BstHHI	GCGC	3 cut(s)	418, 478, 640
BstKTI	GATC	7 cut(s)	112, 172, 217, 432, 466, 630, 700
BstMAI	GTCTC	2 cut(s)	482, 639
BstMBI	GATC	7 cut(s)	109, 169, 214, 429, 463, 627, 697
BstMWI	GCNNNNNNNGC	2 cut(s)	15, 713
BstNI	CCWGG	2 cut(s)	178, 356
BstSCI	CCNGG	2 cut(s)	176, 354
BstSFI	CTRYAG	2 cut(s)	581, 679
BstSLI	GKGCMC	1 cut(s)	155
BstUI	CGCG	1 cut(s)	638
BstV1I	GCAGC	5 cut(s)	21, 35, 64, 400, 567
BstX2I	RGATCY	4 cut(s)	169, 214, 429, 697
BstYI	RGATCY	4 cut(s)	169, 214, 429, 697
BtgZI	GCGATG	1 cut(s)	64
BtsCI	GGATG	3 cut(s)	192, 364, 570
BtsIMutI	CAGTG	2 cut(s)	153, 390
Cac8I	GCNNGC	1 cut(s)	231
CfoI	GCGC	3 cut(s)	418, 478, 640
CseI	GACGC	2 cut(s)	110, 326
Csp6I	GTAC	1 cut(s)	247
CviAII	CATG	1 cut(s)	521
CviJI	RGCY	8 cut(s)	55, 164, 229, 233, 367, 580, 675, 716
CviKI_1	RGCY	8 cut(s)	55, 164, 229, 233, 367, 580, 675, 716
CviQI	GTAC	1 cut(s)	247
DdeI	CTNAG	2 cut(s)	393, 687
DpnI	GATC	7 cut(s)	111, 171, 216, 431, 465, 629, 699
DpnII	GATC	7 cut(s)	109, 169, 214, 429, 463, 627, 697
EciI	GGCGGA	1 cut(s)	107
Eco24I	GRGCYC	1 cut(s)	166
Eco31I	GGTCTC	1 cut(s)	639
Eco32I	GATATC	1 cut(s)	190
EcoRII	CCWGG	2 cut(s)	176, 354
EcoRV	GATATC	1 cut(s)	190
EcoT38I	GRGCYC	1 cut(s)	166
FaeI	CATG	1 cut(s)	524
FaiI	YATR	8 cut(s)	328, 504, 522, 681, 713, 720, 731, 733
FatI	CATG	1 cut(s)	520
FauNDI	CATATG	1 cut(s)	731
FblI	GTMKAC	2 cut(s)	96, 315
Fnu4HI	GCNGC	5 cut(s)	10, 24, 53, 414, 581
FokI	GGATG	3 cut(s)	179, 371, 577
FriOI	GRGCYC	1 cut(s)	166
Fsp4HI	GCNGC	5 cut(s)	10, 24, 53, 414, 581
FspBI	CTAG	1 cut(s)	459
GlaI	GCGC	3 cut(s)	417, 477, 639
GluI	GCNGC	5 cut(s)	10, 24, 53, 414, 581
GsaI	CCCAGC	1 cut(s)	367
HapII	CCGG	1 cut(s)	613
HgaI	GACGC	2 cut(s)	110, 326
HhaI	GCGC	3 cut(s)	418, 478, 640
Hin1I	GRCGYC	2 cut(s)	69, 102
Hin1II	CATG	1 cut(s)	524
Hin6I	GCGC	3 cut(s)	416, 476, 638
HinP1I	GCGC	3 cut(s)	416, 476, 638
HincII	GTYRAC	4 cut(s)	97, 316, 346, 484
HindII	GTYRAC	4 cut(s)	97, 316, 346, 484
HinfI	GANTC	4 cut(s)	40, 46, 421, 602
HpaII	CCGG	1 cut(s)	613
HphI	GGTGA	1 cut(s)	553
Hpy166II	GTNNAC	4 cut(s)	97, 316, 346, 484
Hpy188I	TCNGA	4 cut(s)	45, 141, 214, 564
Hpy188III	TCNNGA	5 cut(s)	218, 275, 425, 459, 701
Hpy8I	GTNNAC	4 cut(s)	97, 316, 346, 484
Hpy99I	CGWCG	5 cut(s)	71, 101, 104, 295, 320
HpyAV	CCTTC	3 cut(s)	115, 437, 562
HpyCH4III	ACNGT	1 cut(s)	450
HpyCH4IV	ACGT	2 cut(s)	69, 261
HpyCH4V	TGCA	3 cut(s)	338, 496, 583
HpyF10VI	GCNNNNNNNGC	2 cut(s)	15, 713
HpyF3I	CTNAG	2 cut(s)	393, 687
HpySE526I	ACGT	2 cut(s)	69, 261
Hsp92I	GRCGYC	2 cut(s)	69, 102
Hsp92II	CATG	1 cut(s)	524
HspAI	GCGC	3 cut(s)	416, 476, 638
Kzo9I	GATC	7 cut(s)	109, 169, 214, 429, 463, 627, 697
LmnI	GCTCC	1 cut(s)	241
Lsp1109I	GCAGC	5 cut(s)	21, 35, 64, 400, 567
LweI	GCATC	3 cut(s)	137, 349, 560
MaeI	CTAG	1 cut(s)	459
MaeII	ACGT	2 cut(s)	69, 261
MaeIII	GTNAC	1 cut(s)	655
MalI	GATC	7 cut(s)	111, 171, 216, 431, 465, 629, 699
MboI	GATC	7 cut(s)	109, 169, 214, 429, 463, 627, 697
MboII	GAAGA	4 cut(s)	307, 611, 628, 707
MflI	RGATCY	4 cut(s)	169, 214, 429, 697
MhlI	GDGCHC	2 cut(s)	155, 166
MluCI	AATT	4 cut(s)	31, 144, 526, 663
MmeI	TCCRAC	1 cut(s)	672
MnlI	CCTC	7 cut(s)	13, 142, 145, 271, 372, 388, 665
MseI	TTAA	1 cut(s)	74
MspA1I	CMGCKG	2 cut(s)	55, 367
MspI	CCGG	1 cut(s)	613
MspR9I	CCNGG	2 cut(s)	178, 356
Mva1269I	GAATGC	2 cut(s)	26, 415
MvaI	CCWGG	2 cut(s)	178, 356
MvnI	CGCG	1 cut(s)	638
MwoI	GCNNNNNNNGC	2 cut(s)	15, 713
NdeI	CATATG	1 cut(s)	731
NdeII	GATC	7 cut(s)	109, 169, 214, 429, 463, 627, 697
NlaIII	CATG	1 cut(s)	524
PcsI	WCGNNNNNNNCGW	1 cut(s)	105
PctI	GAATGC	2 cut(s)	26, 415
PfeI	GAWTC	4 cut(s)	40, 46, 421, 602
PflFI	GACNNNGTC	1 cut(s)	92
PflMI	CCANNNNNTGG	1 cut(s)	538
PkrI	GCNGC	5 cut(s)	11, 25, 54, 415, 582
Psp6I	CCWGG	2 cut(s)	176, 354
PspFI	CCCAGC	1 cut(s)	363
PspGI	CCWGG	2 cut(s)	176, 354
PstI	CTGCAG	1 cut(s)	585
PsuI	RGATCY	4 cut(s)	169, 214, 429, 697
PsyI	GACNNNGTC	1 cut(s)	92
PvuII	CAGCTG	2 cut(s)	55, 367
RsaI	GTAC	1 cut(s)	248
RsaNI	GTAC	1 cut(s)	247
SalI	GTCGAC	2 cut(s)	95, 314
SaqAI	TTAA	1 cut(s)	74
SatI	GCNGC	5 cut(s)	10, 24, 53, 414, 581
Sau3AI	GATC	7 cut(s)	109, 169, 214, 429, 463, 627, 697
ScaI	AGTACT	1 cut(s)	248
ScrFI	CCNGG	2 cut(s)	178, 356
SduI	GDGCHC	2 cut(s)	155, 166
SfaNI	GCATC	3 cut(s)	137, 349, 560
SfcI	CTRYAG	2 cut(s)	581, 679
Sse9I	AATT	4 cut(s)	31, 144, 526, 663
SsiI	CCGC	3 cut(s)	50, 118, 196
SspMI	CTAG	1 cut(s)	459
StyD4I	CCNGG	2 cut(s)	176, 354
TaaI	ACNGT	1 cut(s)	450
TaiI	ACGT	2 cut(s)	72, 264
TaqI	TCGA	6 cut(s)	96, 108, 285, 293, 315, 424
TaqII	GACCGA	1 cut(s)	602
TasI	AATT	4 cut(s)	31, 144, 526, 663
TatI	WGTACW	1 cut(s)	246
TfiI	GAWTC	4 cut(s)	40, 46, 421, 602
Tru1I	TTAA	1 cut(s)	74
Tru9I	TTAA	1 cut(s)	74
TscAI	CASTG	2 cut(s)	160, 397
TseI	GCWGC	5 cut(s)	9, 23, 52, 413, 580
TspDTI	ATGAA	4 cut(s)	49, 318, 537, 581
TspGWI	ACGGA	1 cut(s)	674
TspRI	CASTG	2 cut(s)	160, 397
Tth111I	GACNNNGTC	1 cut(s)	92
Van91I	CCANNNNNTGG	1 cut(s)	538
XapI	RAATTY	2 cut(s)	526, 663
XbaI	TCTAGA	1 cut(s)	458
XmiI	GTMKAC	2 cut(s)	96, 315
XspI	CTAG	1 cut(s)	459
ZraI	GACGTC	1 cut(s)	70
ZrmI	AGTACT	1 cut(s)	248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.