Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0011148

Physical Location & Seq

Genomic Coordinates Reverse (-)

34217918 .. 34219997

Gene Structure 2080 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1208100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 552 bp

ATGGAAGGCAGTGAAAGCGAGGCAGCTTTCAATTCGCTGAATCTGAATCCGCAGCTGTTCATCAACGACGTCTTAACGTCGCCGATGACTTGGTCGACGACGCTTTCGATCATTTCCACCACATCAACCTTTCTGAAAACTGAGGGCACTGAGAGAGCCCAAGATCTATCCAGGGGTGTTGATATCATCCGCAAAAAGGTTCAATCAGATCTTGATAAGAAGCTGGCCGTCTGGGAGCAGTACTGCCTGGACTACGTTTTTCGAGTTCCAGAGGGGTTCGACCCGGCCAAGGATATATTTGACGCAGACCTGGATGCCGAGCTGGATTTGTTGAGGAATGAACTTACTGAGGTTGGTAAGGAGAATGCTGCGCTGAATCGAGAGATCCAATCGTTAGAAGGACAGTCTGCTTCGAGAGATCGTTGTTCTGCGCTTGTCAATGCGACCTTGCAACAATATGAACTAAACTCTTACCATGAGATGTTCCAGGGCTTCTCGAATGCTAAGTTGGAAGATCTTCAAGCGTTTATAGCCCATATGAAAAACATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000070 GO:0000228 GO:0000278 GO:0000280 GO:0000444 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000818 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006996 GO:0007049 GO:0007059 GO:0008104 GO:0008150 GO:0009987 GO:0016043 GO:0022402 GO:0022607 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0033365 GO:0034501 GO:0034502 GO:0034508 GO:0034613 GO:0034622 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0048285 GO:0051179 GO:0051276 GO:0051382 GO:0051383 GO:0051641 GO:0065003 GO:0065004 GO:0070013 GO:0070727 GO:0070925 GO:0071459 GO:0071824 GO:0071840 GO:0098687 GO:0098813 GO:0140014 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mis12

Protein Analysis

184

Amino Acids

20.67

Weight (kDa)

4.52

Isoelectric Point (pI)

37.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mis12	PF05859	9 - 181	4.3e-14	Mis12 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35520
fragaria_vesca	FvH4_5g13000
malus_domestica	MD06G1208100.v1.1 MD14G1218500.v1.1
prunus_persica	Prupe.5G212600_v2.0.a1 Prupe.5G212600_v2.0.a1
pyrus_communis	pycom06g18590 pycom14g18110
rosa_chinensis	RchiOBHm_Chr7g0184161
rosa_laevigata	RLG00000004998
rosa_multiflora	Rmu_co8235265.1_g000001 Rmu_sc0012221.1_g000004
rosa_roxburghii	Rroxscaffold_3G00269840
rosa_rugosa	Rorug06G0463400 Rorug06G0463500
rosa_samantha	Rh7AG082400 Rh7BG064800 Rh7CG065200 Rh7DG064900
rosa_wichuraiana	Rw7G005310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	72
AccI	GTMKAC	1 cut(s)	95
AciI	CCGC	2 cut(s)	50, 190
AclWI	GGATC	1 cut(s)	379
AcoI	YGGCCR	2 cut(s)	225, 285
AcyI	GRCGYC	1 cut(s)	69
AfaI	GTAC	1 cut(s)	242
AfiI	CCNNNNNNNGG	2 cut(s)	196, 289
AflIII	ACRYGT	1 cut(s)	546
AgsI	TTSAA	3 cut(s)	31, 203, 521
AjnI	CCWGG	4 cut(s)	170, 246, 309, 486
AjuI	GAANNNNNNNTTGG	2 cut(s)	491, 523
AluBI	AGCT	4 cut(s)	26, 55, 223, 322
AluI	AGCT	4 cut(s)	26, 55, 223, 322
AlwI	GGATC	1 cut(s)	379
AoxI	GGCC	2 cut(s)	225, 285
ApeKI	GCWGC	3 cut(s)	23, 52, 368
ArsI	GACNNNNNNTTYG	2 cut(s)	88, 120
Asp700I	GAANNNNTTC	1 cut(s)	516
AspLEI	GCGC	2 cut(s)	373, 433
AsuC2I	CCSGG	1 cut(s)	284
BaeGI	GKGCMC	1 cut(s)	149
BanII	GRGCYC	1 cut(s)	160
BbvI	GCAGC	3 cut(s)	35, 64, 355
BceAI	ACGGC	1 cut(s)	212
BciT130I	CCWGG	4 cut(s)	172, 248, 311, 488
BcnI	CCSGG	1 cut(s)	284
BglII	AGATCT	3 cut(s)	163, 208, 514
BisI	GCNGC	3 cut(s)	24, 53, 369
BlsI	GCNGC	3 cut(s)	25, 54, 370
BmcAI	AGTACT	1 cut(s)	242
Bme1390I	CCNGG	5 cut(s)	172, 248, 284, 311, 488
BmrFI	CCNGG	5 cut(s)	172, 248, 284, 311, 488
BmsI	GCATC	1 cut(s)	304
BpuMI	CCSGG	1 cut(s)	284
BsaHI	GRCGYC	1 cut(s)	69
BsaJI	CCNNGG	3 cut(s)	171, 288, 487
Bsc4I	CCNNNNNNNGG	2 cut(s)	196, 289
BseBI	CCWGG	4 cut(s)	172, 248, 311, 488
BseDI	CCNNGG	3 cut(s)	171, 288, 487
BseGI	GGATG	2 cut(s)	186, 319
BseLI	CCNNNNNNNGG	2 cut(s)	196, 289
BseMII	CTCAG	3 cut(s)	132, 141, 339
BseSI	GKGCMC	1 cut(s)	149
BseXI	GCAGC	3 cut(s)	35, 64, 355
BshFI	GGCC	2 cut(s)	227, 287
BsiSI	CCGG	1 cut(s)	284
BslI	CCNNNNNNNGG	2 cut(s)	196, 289
BsmI	GAATGC	2 cut(s)	370, 505
BsnI	GGCC	2 cut(s)	227, 287
Bsp1286I	GDGCHC	2 cut(s)	149, 160
Bsp143I	GATC	6 cut(s)	108, 163, 208, 384, 418, 514
BspACI	CCGC	2 cut(s)	50, 190
BspANI	GGCC	2 cut(s)	227, 287
BspCNI	CTCAG	3 cut(s)	133, 142, 340
BspPI	GGATC	1 cut(s)	379
BssECI	CCNNGG	3 cut(s)	171, 288, 487
BssMI	GATC	6 cut(s)	108, 163, 208, 384, 418, 514
BssNI	GRCGYC	1 cut(s)	69
BssT1I	CCWWGG	1 cut(s)	288
Bst2UI	CCWGG	4 cut(s)	172, 248, 311, 488
Bst4CI	ACNGT	1 cut(s)	405
BstACI	GRCGYC	1 cut(s)	69
BstC8I	GCNNGC	1 cut(s)	225
BstDEI	CTNAG	4 cut(s)	141, 150, 348, 504
BstF5I	GGATG	2 cut(s)	186, 319
BstHHI	GCGC	2 cut(s)	373, 433
BstKTI	GATC	6 cut(s)	111, 166, 211, 387, 421, 517
BstMBI	GATC	6 cut(s)	108, 163, 208, 384, 418, 514
BstMWI	GCNNNNNNNGC	2 cut(s)	15, 530
BstNI	CCWGG	4 cut(s)	172, 248, 311, 488
BstNSI	RCATGY	1 cut(s)	550
BstSCI	CCNGG	5 cut(s)	170, 246, 282, 309, 486
BstSLI	GKGCMC	1 cut(s)	149
BstV1I	GCAGC	3 cut(s)	35, 64, 355
BstX2I	RGATCY	4 cut(s)	163, 208, 384, 514
BstYI	RGATCY	4 cut(s)	163, 208, 384, 514
BsuRI	GGCC	2 cut(s)	227, 287
BtsCI	GGATG	2 cut(s)	186, 319
BtsI	GCAGTG	1 cut(s)	16
BtsIMutI	CAGTG	2 cut(s)	16, 147
Cac8I	GCNNGC	1 cut(s)	225
CfoI	GCGC	2 cut(s)	373, 433
CseI	GACGC	2 cut(s)	109, 311
Csp6I	GTAC	1 cut(s)	241
CviAII	CATG	2 cut(s)	476, 547
CviJI	RGCY	9 cut(s)	26, 55, 158, 223, 227, 287, 322, 492, 533
CviKI_1	RGCY	9 cut(s)	26, 55, 158, 223, 227, 287, 322, 492, 533
CviQI	GTAC	1 cut(s)	241
DdeI	CTNAG	4 cut(s)	141, 150, 348, 504
DpnI	GATC	6 cut(s)	110, 165, 210, 386, 420, 516
DpnII	GATC	6 cut(s)	108, 163, 208, 384, 418, 514
EaeI	YGGCCR	2 cut(s)	225, 285
Eco130I	CCWWGG	1 cut(s)	288
Eco24I	GRGCYC	1 cut(s)	160
Eco32I	GATATC	1 cut(s)	184
EcoRII	CCWGG	4 cut(s)	170, 246, 309, 486
EcoRV	GATATC	1 cut(s)	184
EcoT14I	CCWWGG	1 cut(s)	288
EcoT38I	GRGCYC	1 cut(s)	160
ErhI	CCWWGG	1 cut(s)	288
FaeI	CATG	2 cut(s)	479, 550
FaiI	YATR	7 cut(s)	296, 459, 477, 530, 537, 539, 548
FatI	CATG	2 cut(s)	475, 546
FauNDI	CATATG	1 cut(s)	537
FblI	GTMKAC	1 cut(s)	95
Fnu4HI	GCNGC	3 cut(s)	24, 53, 369
FokI	GGATG	2 cut(s)	173, 326
FriOI	GRGCYC	1 cut(s)	160
Fsp4HI	GCNGC	3 cut(s)	24, 53, 369
GlaI	GCGC	2 cut(s)	372, 432
GluI	GCNGC	3 cut(s)	24, 53, 369
HaeIII	GGCC	2 cut(s)	227, 287
HapII	CCGG	1 cut(s)	284
HgaI	GACGC	2 cut(s)	109, 311
HhaI	GCGC	2 cut(s)	373, 433
Hin1I	GRCGYC	1 cut(s)	69
Hin1II	CATG	2 cut(s)	479, 550
Hin6I	GCGC	2 cut(s)	371, 431
HinP1I	GCGC	2 cut(s)	371, 431
HincII	GTYRAC	1 cut(s)	96
HindII	GTYRAC	1 cut(s)	96
HinfI	GANTC	3 cut(s)	40, 46, 376
HpaII	CCGG	1 cut(s)	284
Hpy166II	GTNNAC	1 cut(s)	96
Hpy188I	TCNGA	3 cut(s)	45, 135, 208
Hpy188III	TCNNGA	5 cut(s)	212, 269, 380, 414, 496
Hpy8I	GTNNAC	1 cut(s)	96
Hpy99I	CGWCG	4 cut(s)	71, 82, 100, 103
HpyAV	CCTTC	1 cut(s)	392
HpyCH4III	ACNGT	1 cut(s)	405
HpyCH4IV	ACGT	3 cut(s)	69, 77, 255
HpyCH4V	TGCA	1 cut(s)	451
HpyF10VI	GCNNNNNNNGC	2 cut(s)	15, 530
HpyF3I	CTNAG	4 cut(s)	141, 150, 348, 504
HpySE526I	ACGT	3 cut(s)	69, 77, 255
Hsp92I	GRCGYC	1 cut(s)	69
Hsp92II	CATG	2 cut(s)	479, 550
HspAI	GCGC	2 cut(s)	371, 431
Kzo9I	GATC	6 cut(s)	108, 163, 208, 384, 418, 514
LmnI	GCTCC	1 cut(s)	235
Lsp1109I	GCAGC	3 cut(s)	35, 64, 355
LweI	GCATC	1 cut(s)	304
MaeII	ACGT	3 cut(s)	69, 77, 255
MalI	GATC	6 cut(s)	110, 165, 210, 386, 420, 516
MboI	GATC	6 cut(s)	108, 163, 208, 384, 418, 514
MboII	GAAGA	2 cut(s)	509, 524
MflI	RGATCY	4 cut(s)	163, 208, 384, 514
MhlI	GDGCHC	2 cut(s)	149, 160
MluCI	AATT	1 cut(s)	31
MmeI	TCCRAC	1 cut(s)	489
MnlI	CCTC	5 cut(s)	13, 136, 265, 327, 343
MroXI	GAANNNNTTC	1 cut(s)	516
MseI	TTAA	1 cut(s)	74
MspA1I	CMGCKG	1 cut(s)	55
MspI	CCGG	1 cut(s)	284
MspR9I	CCNGG	5 cut(s)	172, 248, 284, 311, 488
Mva1269I	GAATGC	2 cut(s)	370, 505
MvaI	CCWGG	4 cut(s)	172, 248, 311, 488
MwoI	GCNNNNNNNGC	2 cut(s)	15, 530
NciI	CCSGG	1 cut(s)	284
NdeI	CATATG	1 cut(s)	537
NdeII	GATC	6 cut(s)	108, 163, 208, 384, 418, 514
NlaIII	CATG	2 cut(s)	479, 550
NmeAIII	GCCGAG	1 cut(s)	343
NspI	RCATGY	1 cut(s)	550
PciI	ACATGT	1 cut(s)	546
PcsI	WCGNNNNNNNCGW	1 cut(s)	104
PctI	GAATGC	2 cut(s)	370, 505
PdmI	GAANNNNTTC	1 cut(s)	516
PfeI	GAWTC	3 cut(s)	40, 46, 376
PflFI	GACNNNGTC	1 cut(s)	91
PkrI	GCNGC	3 cut(s)	25, 54, 370
PscI	ACATGT	1 cut(s)	546
Psp6I	CCWGG	4 cut(s)	170, 246, 309, 486
PspGI	CCWGG	4 cut(s)	170, 246, 309, 486
PsuI	RGATCY	4 cut(s)	163, 208, 384, 514
PsyI	GACNNNGTC	1 cut(s)	91
PvuII	CAGCTG	1 cut(s)	55
RsaI	GTAC	1 cut(s)	242
RsaNI	GTAC	1 cut(s)	241
SalI	GTCGAC	1 cut(s)	94
SaqAI	TTAA	1 cut(s)	74
SatI	GCNGC	3 cut(s)	24, 53, 369
Sau3AI	GATC	6 cut(s)	108, 163, 208, 384, 418, 514
ScaI	AGTACT	1 cut(s)	242
ScrFI	CCNGG	5 cut(s)	172, 248, 284, 311, 488
SduI	GDGCHC	2 cut(s)	149, 160
SfaNI	GCATC	1 cut(s)	304
Sse9I	AATT	1 cut(s)	31
SsiI	CCGC	2 cut(s)	50, 190
StyD4I	CCNGG	5 cut(s)	170, 246, 282, 309, 486
StyI	CCWWGG	1 cut(s)	288
TaaI	ACNGT	1 cut(s)	405
TaiI	ACGT	3 cut(s)	72, 80, 258
TaqI	TCGA	7 cut(s)	95, 107, 262, 279, 379, 413, 497
TasI	AATT	1 cut(s)	31
TatI	WGTACW	1 cut(s)	240
TfiI	GAWTC	3 cut(s)	40, 46, 376
Tru1I	TTAA	1 cut(s)	74
Tru9I	TTAA	1 cut(s)	74
TscAI	CASTG	2 cut(s)	16, 154
TseI	GCWGC	3 cut(s)	23, 52, 368
TspDTI	ATGAA	3 cut(s)	49, 354, 474
TspRI	CASTG	2 cut(s)	16, 154
Tth111I	GACNNNGTC	1 cut(s)	91
XceI	RCATGY	1 cut(s)	550
XmiI	GTMKAC	1 cut(s)	95
XmnI	GAANNNNTTC	1 cut(s)	516
ZraI	GACGTC	1 cut(s)	70
ZrmI	AGTACT	1 cut(s)	242

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.