Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0011148

Physical Location & Seq

Genomic Coordinates Forward (+)

64268048 .. 64269261

Gene Structure 1214 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000004998

Sequence Viewer

CDS Protein Promoter (2k)

Length: 543 bp

ATGGAACCGAGCGAAAGCGAGGCGGTGTTTGATTCGGTGAACCTGAATCCGCAGCTGTTCATCAACGAGGTCATCAACACCGTCGACGATTTGGTCGACGATGCGTTCGATTACTTCCAGCAGGAAGCGTCGACCTCTTTGAAAGTCGAAGGCACTGATAGAGCTCAAGATCTAGCCAAGGGCGTTAATCTCATTCACAAAATGGTTCAATCAAATGTTGATAAGCGGCTGGTTATGTGGGAGCGTTACTGTCTTGACCATTGTTTTGAAGTTCCTGAGGGATTTGTGCTGCCCAAAAGTGGTGAAACACCTGTTGATAGTTCTGTATGTCAAAATGCAAGCTGTGATCCAGAAGTGGATGCCCAGTTGGATTCGTTGAGGAACAAACTCACCAAGGTTGGTGAGGAATCTGCCGCACTGAACCGAGAGCTCCAATTATTGGAACAACAGTCTGCTTCTAGTGACCGTTTATCTGCGCTTGTCAATGAGACATTGCAGTTATATGACCGAAACTCTTTTCATGAGATGTTCCAGGGTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000070 GO:0000228 GO:0000278 GO:0000280 GO:0000444 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000818 GO:0000819 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006996 GO:0007049 GO:0007059 GO:0008104 GO:0008150 GO:0009987 GO:0016043 GO:0022402 GO:0022607 GO:0031974 GO:0031981 GO:0032991 GO:0033036 GO:0033365 GO:0034501 GO:0034502 GO:0034508 GO:0034613 GO:0034622 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0048285 GO:0051179 GO:0051276 GO:0051382 GO:0051383 GO:0051641 GO:0065003 GO:0065004 GO:0070013 GO:0070727 GO:0070925 GO:0071459 GO:0071824 GO:0071840 GO:0098687 GO:0098813 GO:0140014 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Mis12

Protein Analysis

181

Amino Acids

20.24

Weight (kDa)

4.29

Isoelectric Point (pI)

47.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Mis12	PF05859	8 - 176	6.2e-32	Mis12 protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G35520
fragaria_vesca	FvH4_5g13000
malus_domestica	MD06G1208100.v1.1 MD14G1218500.v1.1
prunus_persica	Prupe.5G212600_v2.0.a1 Prupe.5G212600_v2.0.a1
pyrus_communis	pycom06g18590 pycom14g18110
rosa_chinensis	RchiOBHm_Chr7g0184161
rosa_laevigata	RLG00000004998
rosa_multiflora	Rmu_co8235265.1_g000001 Rmu_sc0012221.1_g000004
rosa_roxburghii	Rroxscaffold_3G00269840
rosa_rugosa	Rorug06G0463400 Rorug06G0463500
rosa_samantha	Rh7AG082400 Rh7BG064800 Rh7CG065200 Rh7DG064900
rosa_wichuraiana	Rw7G005310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	92
AccB7I	CCANNNNNTGG	1 cut(s)	439
AccI	GTMKAC	3 cut(s)	84, 96, 131
AciI	CCGC	4 cut(s)	23, 50, 226, 414
AclWI	GGATC	1 cut(s)	341
AfiI	CCNNNNNNNGG	2 cut(s)	299, 439
AgsI	TTSAA	3 cut(s)	142, 209, 269
AjnI	CCWGG	1 cut(s)	531
AluBI	AGCT	4 cut(s)	55, 164, 342, 430
AluI	AGCT	4 cut(s)	55, 164, 342, 430
Alw21I	GWGCWC	2 cut(s)	166, 432
Alw26I	GTCTC	1 cut(s)	482
AlwI	GGATC	1 cut(s)	341
ApeKI	GCWGC	2 cut(s)	52, 289
ArsI	GACNNNNNNTTYG	4 cut(s)	89, 121, 248, 280
AspLEI	GCGC	1 cut(s)	478
AsuHPI	GGTGA	4 cut(s)	49, 314, 382, 413
AxyI	CCTNAGG	1 cut(s)	276
BanII	GRGCYC	2 cut(s)	166, 432
Bbv12I	GWGCWC	2 cut(s)	166, 432
BbvI	GCAGC	2 cut(s)	64, 276
BciT130I	CCWGG	1 cut(s)	533
BcoDI	GTCTC	1 cut(s)	482
BfaI	CTAG	2 cut(s)	173, 459
BglII	AGATCT	1 cut(s)	169
BisI	GCNGC	4 cut(s)	53, 227, 290, 414
BlsI	GCNGC	4 cut(s)	54, 228, 291, 415
Bme1390I	CCNGG	1 cut(s)	533
BmiI	GGNNCC	1 cut(s)	6
BmrFI	CCNGG	1 cut(s)	533
BmrI	ACTGGG	1 cut(s)	358
BmsI	GCATC	2 cut(s)	91, 349
BmuI	ACTGGG	1 cut(s)	358
BpuEI	CTTGAG	1 cut(s)	150
BsaJI	CCNNGG	3 cut(s)	177, 393, 532
Bsc4I	CCNNNNNNNGG	2 cut(s)	299, 439
Bse1I	ACTGG	1 cut(s)	364
Bse21I	CCTNAGG	1 cut(s)	276
Bse3DI	GCAATG	1 cut(s)	491
BseBI	CCWGG	1 cut(s)	533
BseDI	CCNNGG	3 cut(s)	177, 393, 532
BseGI	GGATG	1 cut(s)	364
BseLI	CCNNNNNNNGG	2 cut(s)	299, 439
BseMI	GCAATG	1 cut(s)	491
BseMII	CTCAG	1 cut(s)	267
BseNI	ACTGG	1 cut(s)	364
BseXI	GCAGC	2 cut(s)	64, 276
BsiHKAI	GWGCWC	2 cut(s)	166, 432
BslI	CCNNNNNNNGG	2 cut(s)	299, 439
BsmAI	GTCTC	1 cut(s)	482
Bsp1286I	GDGCHC	2 cut(s)	166, 432
Bsp143I	GATC	2 cut(s)	169, 346
BspACI	CCGC	4 cut(s)	23, 50, 226, 414
BspCNI	CTCAG	1 cut(s)	268
BspHI	TCATGA	1 cut(s)	520
BspLI	GGNNCC	1 cut(s)	6
BspPI	GGATC	1 cut(s)	341
BsrDI	GCAATG	1 cut(s)	491
BsrI	ACTGG	1 cut(s)	364
BssECI	CCNNGG	3 cut(s)	177, 393, 532
BssMI	GATC	2 cut(s)	169, 346
BssT1I	CCWWGG	2 cut(s)	177, 393
Bst2UI	CCWGG	1 cut(s)	533
Bst4CI	ACNGT	4 cut(s)	82, 251, 450, 467
BstC8I	GCNNGC	1 cut(s)	340
BstDEI	CTNAG	1 cut(s)	276
BstF5I	GGATG	1 cut(s)	364
BstHHI	GCGC	1 cut(s)	478
BstKTI	GATC	2 cut(s)	172, 349
BstMAI	GTCTC	1 cut(s)	482
BstMBI	GATC	2 cut(s)	169, 346
BstNI	CCWGG	1 cut(s)	533
BstSCI	CCNGG	1 cut(s)	531
BstV1I	GCAGC	2 cut(s)	64, 276
BstX2I	RGATCY	1 cut(s)	169
BstYI	RGATCY	1 cut(s)	169
Bsu36I	CCTNAGG	1 cut(s)	276
BtsCI	GGATG	1 cut(s)	364
BtsIMutI	CAGTG	2 cut(s)	153, 416
Cac8I	GCNNGC	1 cut(s)	340
CciI	TCATGA	1 cut(s)	520
CfoI	GCGC	1 cut(s)	478
CseI	GACGC	1 cut(s)	117
CviAII	CATG	1 cut(s)	521
CviJI	RGCY	6 cut(s)	55, 164, 176, 229, 342, 430
CviKI_1	RGCY	6 cut(s)	55, 164, 176, 229, 342, 430
DdeI	CTNAG	1 cut(s)	276
DpnI	GATC	2 cut(s)	171, 348
DpnII	GATC	2 cut(s)	169, 346
DrdI	GACNNNNNNGTC	1 cut(s)	92
DseDI	GACNNNNNNGTC	1 cut(s)	92
Ecl136II	GAGCTC	2 cut(s)	164, 430
Eco130I	CCWWGG	2 cut(s)	177, 393
Eco24I	GRGCYC	2 cut(s)	166, 432
Eco53kI	GAGCTC	2 cut(s)	164, 430
Eco81I	CCTNAGG	1 cut(s)	276
EcoICRI	GAGCTC	2 cut(s)	164, 430
EcoRII	CCWGG	1 cut(s)	531
EcoT14I	CCWWGG	2 cut(s)	177, 393
EcoT38I	GRGCYC	2 cut(s)	166, 432
ErhI	CCWWGG	2 cut(s)	177, 393
FaeI	CATG	1 cut(s)	524
FaiI	YATR	5 cut(s)	236, 328, 502, 504, 522
FatI	CATG	1 cut(s)	520
FblI	GTMKAC	3 cut(s)	84, 96, 131
Fnu4HI	GCNGC	4 cut(s)	53, 227, 290, 414
FokI	GGATG	1 cut(s)	371
FriOI	GRGCYC	2 cut(s)	166, 432
Fsp4HI	GCNGC	4 cut(s)	53, 227, 290, 414
FspBI	CTAG	2 cut(s)	173, 459
GlaI	GCGC	1 cut(s)	477
GluI	GCNGC	4 cut(s)	53, 227, 290, 414
HgaI	GACGC	1 cut(s)	117
HhaI	GCGC	1 cut(s)	478
Hin1II	CATG	1 cut(s)	524
Hin6I	GCGC	1 cut(s)	476
HinP1I	GCGC	1 cut(s)	476
HincII	GTYRAC	3 cut(s)	85, 97, 132
HindII	GTYRAC	3 cut(s)	85, 97, 132
HinfI	GANTC	4 cut(s)	32, 46, 371, 407
HphI	GGTGA	4 cut(s)	49, 314, 382, 413
Hpy166II	GTNNAC	4 cut(s)	40, 85, 97, 132
Hpy188III	TCNNGA	5 cut(s)	167, 254, 275, 350, 521
Hpy8I	GTNNAC	4 cut(s)	40, 85, 97, 132
Hpy99I	CGWCG	4 cut(s)	86, 89, 101, 133
HpyAV	CCTTC	1 cut(s)	143
HpyCH4III	ACNGT	4 cut(s)	82, 251, 450, 467
HpyCH4V	TGCA	2 cut(s)	338, 496
HpyF3I	CTNAG	1 cut(s)	276
Hsp92II	CATG	1 cut(s)	524
HspAI	GCGC	1 cut(s)	476
Kzo9I	GATC	2 cut(s)	169, 346
LmnI	GCTCC	2 cut(s)	241, 435
LpnPI	CCDG	9 cut(s)	56, 107, 131, 215, 288, 324, 363, 377, 518
Lsp1109I	GCAGC	2 cut(s)	64, 276
LweI	GCATC	2 cut(s)	91, 349
MaeI	CTAG	2 cut(s)	173, 459
MaeIII	GTNAC	2 cut(s)	245, 461
MalI	GATC	2 cut(s)	171, 348
MboI	GATC	2 cut(s)	169, 346
MflI	RGATCY	1 cut(s)	169
MhlI	GDGCHC	2 cut(s)	166, 432
MluCI	AATT	2 cut(s)	434, 538
MmeI	TCCRAC	1 cut(s)	348
MnlI	CCTC	6 cut(s)	13, 61, 145, 271, 372, 397
MseI	TTAA	2 cut(s)	186, 541
MspA1I	CMGCKG	1 cut(s)	55
MspR9I	CCNGG	1 cut(s)	533
MvaI	CCWGG	1 cut(s)	533
NdeII	GATC	2 cut(s)	169, 346
NlaIII	CATG	1 cut(s)	524
NlaIV	GGNNCC	1 cut(s)	6
NmuCI	GTSAC	1 cut(s)	461
PagI	TCATGA	1 cut(s)	520
PcsI	WCGNNNNNNNCGW	2 cut(s)	93, 105
PfeI	GAWTC	4 cut(s)	32, 46, 371, 407
PflMI	CCANNNNNTGG	1 cut(s)	439
PkrI	GCNGC	4 cut(s)	54, 228, 291, 415
Psp124BI	GAGCTC	2 cut(s)	166, 432
Psp6I	CCWGG	1 cut(s)	531
PspGI	CCWGG	1 cut(s)	531
PspN4I	GGNNCC	1 cut(s)	6
PsuI	RGATCY	1 cut(s)	169
PvuII	CAGCTG	1 cut(s)	55
SacI	GAGCTC	2 cut(s)	166, 432
SalI	GTCGAC	3 cut(s)	83, 95, 130
SaqAI	TTAA	2 cut(s)	186, 541
SatI	GCNGC	4 cut(s)	53, 227, 290, 414
Sau3AI	GATC	2 cut(s)	169, 346
ScrFI	CCNGG	1 cut(s)	533
SduI	GDGCHC	2 cut(s)	166, 432
SetI	ASST	9 cut(s)	45, 57, 72, 137, 166, 313, 344, 399, 432
SfaNI	GCATC	2 cut(s)	91, 349
SgrDI	CGTCGACG	1 cut(s)	83
SmlI	CTYRAG	1 cut(s)	165
SmoI	CTYRAG	1 cut(s)	165
Sse9I	AATT	2 cut(s)	434, 538
SsiI	CCGC	4 cut(s)	23, 50, 226, 414
SspMI	CTAG	2 cut(s)	173, 459
SstI	GAGCTC	2 cut(s)	166, 432
StyD4I	CCNGG	1 cut(s)	531
StyI	CCWWGG	2 cut(s)	177, 393
TaaI	ACNGT	4 cut(s)	82, 251, 450, 467
TaqI	TCGA	5 cut(s)	84, 96, 108, 131, 147
TaqII	GACCGA	1 cut(s)	522
TasI	AATT	2 cut(s)	434, 538
TauI	GCSGC	2 cut(s)	229, 416
TfiI	GAWTC	4 cut(s)	32, 46, 371, 407
Tru1I	TTAA	2 cut(s)	186, 541
Tru9I	TTAA	2 cut(s)	186, 541
TscAI	CASTG	2 cut(s)	160, 423
TseFI	GTSAC	1 cut(s)	461
TseI	GCWGC	2 cut(s)	52, 289
Tsp45I	GTSAC	1 cut(s)	461
TspDTI	ATGAA	2 cut(s)	49, 509
TspRI	CASTG	2 cut(s)	160, 423
Van91I	CCANNNNNTGG	1 cut(s)	439
XmiI	GTMKAC	3 cut(s)	84, 96, 131
XspI	CTAG	2 cut(s)	173, 459

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.