AT5G40300

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009012
Physical Location & Seq
Forward (+)
16110634 .. 16113059
2426 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G40300.1

Sequence Viewer

Length: 813 bp
ATGGAAGAATTAGAAAAAACCCAGAAATTTCAGAAGAAGAAGAAGCAACAACAAGAGAAACAAGACCAGTCTTCACCAATCAACTTCGAGATGTCTTCAAGATCATCACTTCATTCATTACCACAAACAACGATTGAATCACCTCCTGATTCACCAACCCTCTCTTCAATCCCCGATAGCCATGGATCTTCTCCTCACACGATTATTCCAACTCCTTCTGTGGCCAAGACGGAGACTCCTTTTAGGGTTACTAACGGAGAGGAGGAGAAGAAAGTCAGCGAGAGTAGGAGACAATTGAGACCGAGCTTTTCTTCTTCTTCTTCTACTCCGCGTGAATCGAAATGGGCAAGTTTGATTAGGAAAGCTCTACTTGGATTTAGGGTTATTGCGTTTGTTTCGTGTCTTGTTTCGTTCTCTGTAATGGTTTCTGATAGAGATAAAGGATGGGCTCATGATTCTTTCTACAACTACAAAGAATTCAGGTTCTGTTTGGCTGCGAATGTGATTGGTTTTGTCTACTCTGGTTTTATGATATGTGATCTTGTATACCTGTTATCTACTAGCATCCGAAGATCACGACATAACCTGCGACATTTCTTGGAATTTGGTCTTGATCAAATGCTAGCTTATCTTCTTGCATCAGCTTCAACTTCAGCTTCGATCCGCGTAGATGATTGGCAATCAAACTGGGGAGCAGACAAGTTCCCAGACTTAGCTAGAGCATCTGTCGCACTGTCTTACGTCTCCTTTGTCGCATTCGCCTTCTGCTCATTGGCTTCCGGTTATGCTCTATGTGCACTCCGGTCCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

270

Amino Acids

30.3

Weight (kDa)

9.07

Isoelectric Point (pI)

60.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 118 - 252 6.7e-29 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 594
AccI GTMKAC 2 cut(s) 516, 546
AccII CGCG 2 cut(s) 331, 666
AciI CCGC 2 cut(s) 329, 664
AclWI GGATC 2 cut(s) 193, 655
AcoI YGGCCR 1 cut(s) 222
AcsI RAATTY 3 cut(s) 26, 476, 602
AcuI CTGAAG 1 cut(s) 636
AgsI TTSAA 4 cut(s) 99, 137, 168, 648
AjuI GAANNNNNNNTTGG 2 cut(s) 148, 180
AluBI AGCT 6 cut(s) 306, 365, 626, 644, 656, 716
AluI AGCT 6 cut(s) 306, 365, 626, 644, 656, 716
Alw21I GWGCWC 1 cut(s) 799
Alw26I GTCTC 4 cut(s) 227, 283, 292, 748
Alw44I GTGCAC 1 cut(s) 795
AlwI GGATC 2 cut(s) 193, 655
AlwNI CAGNNNCTG 1 cut(s) 486
AoxI GGCC 1 cut(s) 222
ApaLI GTGCAC 1 cut(s) 795
ApeKI GCWGC 1 cut(s) 494
ApoI RAATTY 3 cut(s) 26, 476, 602
AspS9I GGNCC 1 cut(s) 804
AsuHPI GGTGA 3 cut(s) 66, 132, 144
AsuNHI GCTAGC 1 cut(s) 622
AvaII GGWCC 1 cut(s) 804
BaeGI GKGCMC 1 cut(s) 799
BalI TGGCCA 1 cut(s) 224
BanII GRGCYC 1 cut(s) 451
BbsI GAAGAC 2 cut(s) 63, 87
Bbv12I GWGCWC 1 cut(s) 799
BbvI GCAGC 1 cut(s) 481
BccI CCATC 1 cut(s) 438
BclI TGATCA 1 cut(s) 613
BcoDI GTCTC 4 cut(s) 227, 283, 292, 748
BfaI CTAG 3 cut(s) 561, 623, 717
BfuAI ACCTGC 1 cut(s) 594
BisI GCNGC 1 cut(s) 495
BlsI GCNGC 1 cut(s) 496
Bme18I GGWCC 1 cut(s) 804
BmgT120I GGNCC 1 cut(s) 804
BmrI ACTGGG 1 cut(s) 697
BmsI GCATC 3 cut(s) 573, 647, 731
BmtI GCTAGC 1 cut(s) 626
BmuI ACTGGG 1 cut(s) 697
BpiI GAAGAC 2 cut(s) 63, 87
BsaI GGTCTC 1 cut(s) 292
BsaJI CCNNGG 1 cut(s) 181
BsaWI WCCGGW 2 cut(s) 779, 801
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bse1I ACTGG 2 cut(s) 67, 692
BseDI CCNNGG 1 cut(s) 181
BseGI GGATG 2 cut(s) 449, 564
BseNI ACTGG 2 cut(s) 67, 692
BseRI GAGGAG 3 cut(s) 183, 275, 278
BseSI GKGCMC 1 cut(s) 799
BseXI GCAGC 1 cut(s) 481
Bsh1236I CGCG 2 cut(s) 331, 666
BshFI GGCC 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 799
BsiSI CCGG 2 cut(s) 780, 802
BsmAI GTCTC 4 cut(s) 227, 283, 292, 748
BsmBI CGTCTC 1 cut(s) 748
BsmI GAATGC 1 cut(s) 755
BsnI GGCC 1 cut(s) 224
Bso31I GGTCTC 1 cut(s) 292
Bsp1286I GDGCHC 2 cut(s) 451, 799
Bsp143I GATC 6 cut(s) 101, 185, 538, 572, 613, 660
Bsp19I CCATGG 1 cut(s) 181
BspACI CCGC 2 cut(s) 329, 664
BspANI GGCC 1 cut(s) 224
BspFNI CGCG 2 cut(s) 331, 666
BspHI TCATGA 1 cut(s) 451
BspMI ACCTGC 1 cut(s) 594
BspOI GCTAGC 1 cut(s) 626
BspPI GGATC 2 cut(s) 193, 655
BspTNI GGTCTC 1 cut(s) 292
BsrI ACTGG 2 cut(s) 67, 692
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 6 cut(s) 101, 185, 538, 572, 613, 660
BssNAI GTATAC 1 cut(s) 547
BssT1I CCWWGG 1 cut(s) 181
Bst1107I GTATAC 1 cut(s) 547
Bst4CI ACNGT 1 cut(s) 735
Bst6I CTCTTC 1 cut(s) 169
BstC8I GCNNGC 1 cut(s) 624
BstDEI CTNAG 1 cut(s) 712
BstDSI CCRYGG 1 cut(s) 181
BstF5I GGATG 2 cut(s) 449, 564
BstFNI CGCG 2 cut(s) 331, 666
BstKTI GATC 6 cut(s) 104, 188, 541, 575, 616, 663
BstMAI GTCTC 4 cut(s) 227, 283, 292, 748
BstMBI GATC 6 cut(s) 101, 185, 538, 572, 613, 660
BstMWI GCNNNNNNNGC 2 cut(s) 728, 794
BstSLI GKGCMC 1 cut(s) 799
BstUI CGCG 2 cut(s) 331, 666
BstV1I GCAGC 1 cut(s) 481
BstV2I GAAGAC 2 cut(s) 63, 87
BstX2I RGATCY 1 cut(s) 185
BstYI RGATCY 1 cut(s) 185
BstZ17I GTATAC 1 cut(s) 547
BsuRI GGCC 1 cut(s) 224
BtgI CCRYGG 1 cut(s) 181
BtsCI GGATG 2 cut(s) 449, 564
BtsIMutI CAGTG 1 cut(s) 731
BveI ACCTGC 1 cut(s) 594
Cac8I GCNNGC 1 cut(s) 624
CaiI CAGNNNCTG 1 cut(s) 486
CciI TCATGA 1 cut(s) 451
Cfr13I GGNCC 1 cut(s) 804
CviAII CATG 2 cut(s) 182, 452
DdeI CTNAG 1 cut(s) 712
DpnI GATC 6 cut(s) 103, 187, 540, 574, 615, 662
DpnII GATC 6 cut(s) 101, 185, 538, 572, 613, 660
EaeI YGGCCR 1 cut(s) 222
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 181
Eco24I GRGCYC 1 cut(s) 451
Eco31I GGTCTC 1 cut(s) 292
Eco47I GGWCC 1 cut(s) 804
Eco57I CTGAAG 1 cut(s) 636
EcoRI GAATTC 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 181
EcoT38I GRGCYC 1 cut(s) 451
ErhI CCWWGG 1 cut(s) 181
Esp3I CGTCTC 1 cut(s) 748
FaeI CATG 2 cut(s) 185, 455
FaiI YATR 8 cut(s) 183, 453, 530, 535, 547, 582, 786, 793
FalI AAGNNNNNCTT 2 cut(s) 354, 386
FatI CATG 2 cut(s) 181, 451
FbaI TGATCA 1 cut(s) 613
FblI GTMKAC 2 cut(s) 516, 546
Fnu4HI GCNGC 1 cut(s) 495
FokI GGATG 2 cut(s) 456, 551
FriOI GRGCYC 1 cut(s) 451
Fsp4HI GCNGC 1 cut(s) 495
FspBI CTAG 3 cut(s) 561, 623, 717
GluI GCNGC 1 cut(s) 495
HaeIII GGCC 1 cut(s) 224
HapII CCGG 2 cut(s) 780, 802
Hin1II CATG 2 cut(s) 185, 455
HinfI GANTC 5 cut(s) 137, 149, 235, 335, 455
HpaII CCGG 2 cut(s) 780, 802
HphI GGTGA 3 cut(s) 66, 132, 144
Hpy166II GTNNAC 3 cut(s) 517, 547, 797
Hpy188I TCNGA 3 cut(s) 33, 430, 569
Hpy188III TCNNGA 6 cut(s) 88, 99, 146, 452, 576, 611
Hpy8I GTNNAC 3 cut(s) 517, 547, 797
HpyAV CCTTC 2 cut(s) 225, 772
HpyCH4III ACNGT 1 cut(s) 735
HpyCH4IV ACGT 1 cut(s) 741
HpyCH4V TGCA 2 cut(s) 638, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 728, 794
HpyF3I CTNAG 1 cut(s) 712
HpySE526I ACGT 1 cut(s) 741
Hsp92II CATG 2 cut(s) 185, 455
Ksp22I TGATCA 1 cut(s) 613
Kzo9I GATC 6 cut(s) 101, 185, 538, 572, 613, 660
LmnI GCTCC 1 cut(s) 692
Lsp1109I GCAGC 1 cut(s) 481
LweI GCATC 3 cut(s) 573, 647, 731
MaeI CTAG 3 cut(s) 561, 623, 717
MaeII ACGT 1 cut(s) 741
MaeIII GTNAC 1 cut(s) 247
MalI GATC 6 cut(s) 103, 187, 540, 574, 615, 662
MboI GATC 6 cut(s) 101, 185, 538, 572, 613, 660
MfeI CAATTG 1 cut(s) 293
MflI RGATCY 1 cut(s) 185
MhlI GDGCHC 2 cut(s) 451, 799
MlsI TGGCCA 1 cut(s) 224
MluCI AATT 5 cut(s) 8, 26, 293, 476, 602
MluNI TGGCCA 1 cut(s) 224
MlyI GAGTC 1 cut(s) 229
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 5 cut(s) 153, 170, 204, 253, 256
Mox20I TGGCCA 1 cut(s) 224
MscI TGGCCA 1 cut(s) 224
Msp20I TGGCCA 1 cut(s) 224
MspI CCGG 2 cut(s) 780, 802
MunI CAATTG 1 cut(s) 293
Mva1269I GAATGC 1 cut(s) 755
MvnI CGCG 2 cut(s) 331, 666
MwoI GCNNNNNNNGC 2 cut(s) 728, 794
NcoI CCATGG 1 cut(s) 181
NdeII GATC 6 cut(s) 101, 185, 538, 572, 613, 660
NheI GCTAGC 1 cut(s) 622
NlaIII CATG 2 cut(s) 185, 455
PagI TCATGA 1 cut(s) 451
PctI GAATGC 1 cut(s) 755
PfeI GAWTC 4 cut(s) 137, 149, 335, 455
PkrI GCNGC 1 cut(s) 496
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
PspPI GGNCC 1 cut(s) 804
PstNI CAGNNNCTG 1 cut(s) 486
PsuI RGATCY 1 cut(s) 185
SatI GCNGC 1 cut(s) 495
Sau3AI GATC 6 cut(s) 101, 185, 538, 572, 613, 660
Sau96I GGNCC 1 cut(s) 804
SchI GAGTC 1 cut(s) 229
SduI GDGCHC 2 cut(s) 451, 799
SfaNI GCATC 3 cut(s) 573, 647, 731
SinI GGWCC 1 cut(s) 804
Sse9I AATT 5 cut(s) 8, 26, 293, 476, 602
SsiI CCGC 2 cut(s) 329, 664
SspMI CTAG 3 cut(s) 561, 623, 717
StyI CCWWGG 1 cut(s) 181
TaaI ACNGT 1 cut(s) 735
TaiI ACGT 1 cut(s) 744
TaqI TCGA 3 cut(s) 87, 338, 659
TaqII GACCGA 1 cut(s) 316
TasI AATT 5 cut(s) 8, 26, 293, 476, 602
TfiI GAWTC 4 cut(s) 137, 149, 335, 455
TscAI CASTG 1 cut(s) 738
TseI GCWGC 1 cut(s) 494
TspDTI ATGAA 2 cut(s) 101, 105
TspGWI ACGGA 2 cut(s) 245, 270
TspRI CASTG 1 cut(s) 738
VneI GTGCAC 1 cut(s) 795
VpaK11BI GGWCC 1 cut(s) 804
XapI RAATTY 3 cut(s) 26, 476, 602
XmiI GTMKAC 2 cut(s) 516, 546
XspI CTAG 3 cut(s) 561, 623, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.