Rh2AG628700

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0009012
Physical Location & Seq
Forward (+)
85388364 .. 85391396
3033 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG628700.1

Sequence Viewer

Length: 657 bp
ATGAAAAGAGAAACCCAACAAGAATCATCAAAAGACAAAGATGGAGAAGAAAAGCAAGGCATCTCAGAAAAGTCATATTCACCTCCTCTCAGTCCTCTAATATTCCCCCAAAACCATAACAAGTCTCCATCTCCATCTCCATCACCACTACATGATTCTCCAGCTGAAGAAATCTACCACACCAATGAGTTCAGACTCACACCACCGGAGAATCAGACCCCCCACCACTCTAAGTCTGCGCCGCCACCGCCCGTGGAGGCCCAAACCAAAGCCCAAGAAGGGTTTGCTGCGCCAGGAGAGGCTCAAGAGGGAGGAGAGAATAGGAGAACTATGAGACCCTATTTGTCTATATTGAAGAGGGCGAGAAGGCAAAGCGTGGTGAGGAGGGCTATGTTGGGGTTAAGGATTTCTGAGTTTTTGTTTTGTTTGATCTCTTTCTCGGTTATGGCGGCCGACAAGAAGCAGGGTTGGGCTTCAGATTCTTTCTCTAACTACAAGGAGTTCAGGTACTGCTTGGCAGTGAATGTGCTAGGGTTTGTGTATTCAGGGATTCAGACCTATGATTTAGTCTATTACTTCACCACCGGAAAACATGTAGTGCAGCACCATCTCCGATATTACTTCGATTTCTTGATGGATCAGGCAAGTCACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

218

Amino Acids

24.89

Weight (kDa)

8.44

Isoelectric Point (pI)

70.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 126 - 215 1.1e-19 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 242, 248, 449
AclWI GGATC 1 cut(s) 645
AcoI YGGCCR 1 cut(s) 450
AcuI CTGAAG 2 cut(s) 186, 459
AfaI GTAC 1 cut(s) 509
AfiI CCNNNNNNNGG 1 cut(s) 279
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 1 cut(s) 355
AjnI CCWGG 1 cut(s) 292
AluBI AGCT 1 cut(s) 164
AluI AGCT 1 cut(s) 164
Alw26I GTCTC 2 cut(s) 129, 328
AlwI GGATC 1 cut(s) 645
AlwNI CAGNNNCTG 1 cut(s) 510
AoxI GGCC 2 cut(s) 258, 450
ApeKI GCWGC 2 cut(s) 287, 601
AspLEI GCGC 2 cut(s) 241, 292
AspS9I GGNCC 1 cut(s) 259
AsuHPI GGTGA 4 cut(s) 72, 135, 391, 571
BbvI GCAGC 2 cut(s) 274, 613
BccI CCATC 6 cut(s) 35, 136, 142, 148, 615, 628
BciT130I CCWGG 1 cut(s) 294
BcoDI GTCTC 2 cut(s) 129, 328
BfaI CTAG 2 cut(s) 530, 655
BisI GCNGC 4 cut(s) 242, 288, 450, 602
BlsI GCNGC 4 cut(s) 243, 289, 451, 603
Bme1390I CCNGG 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 259
BmrFI CCNGG 1 cut(s) 294
BmsI GCATC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 144
BpuEI CTTGAG 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 252
BsaWI WCCGGW 2 cut(s) 205, 584
BsaXI ACNNNNNCTCC 2 cut(s) 491, 521
Bsc4I CCNNNNNNNGG 1 cut(s) 279
BseBI CCWGG 1 cut(s) 294
BseDI CCNNGG 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 3 cut(s) 78, 103, 402
BseRI GAGGAG 3 cut(s) 75, 327, 397
BseX3I CGGCCG 1 cut(s) 450
BseXI GCAGC 2 cut(s) 274, 613
BsgI GTGCAG 1 cut(s) 620
Bsh1285I CGRYCG 1 cut(s) 453
BshFI GGCC 2 cut(s) 260, 452
BsiEI CGRYCG 1 cut(s) 453
BsiSI CCGG 2 cut(s) 206, 585
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 2 cut(s) 129, 328
BsnI GGCC 2 cut(s) 260, 452
Bso31I GGTCTC 1 cut(s) 328
Bsp143I GATC 2 cut(s) 429, 637
BspACI CCGC 3 cut(s) 242, 248, 449
BspANI GGCC 2 cut(s) 260, 452
BspCNI CTCAG 3 cut(s) 77, 102, 403
BspPI GGATC 1 cut(s) 645
BspTNI GGTCTC 1 cut(s) 328
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 2 cut(s) 429, 637
Bst2UI CCWGG 1 cut(s) 294
Bst6I CTCTTC 1 cut(s) 350
BstDEI CTNAG 4 cut(s) 64, 89, 231, 411
BstDSI CCRYGG 1 cut(s) 252
BstHHI GCGC 2 cut(s) 241, 292
BstKTI GATC 2 cut(s) 432, 640
BstMAI GTCTC 2 cut(s) 129, 328
BstMBI GATC 2 cut(s) 429, 637
BstMCI CGRYCG 1 cut(s) 453
BstMWI GCNNNNNNNGC 1 cut(s) 247
BstNI CCWGG 1 cut(s) 294
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 1 cut(s) 292
BstV1I GCAGC 2 cut(s) 274, 613
BstZI CGGCCG 1 cut(s) 450
BsuRI GGCC 2 cut(s) 260, 452
BtgI CCRYGG 1 cut(s) 252
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 1 cut(s) 525
CaiI CAGNNNCTG 1 cut(s) 510
CfoI GCGC 2 cut(s) 241, 292
Cfr13I GGNCC 1 cut(s) 259
Csp6I GTAC 1 cut(s) 508
CviAII CATG 2 cut(s) 152, 593
CviJI RGCY 7 cut(s) 164, 260, 272, 302, 389, 452, 473
CviKI_1 RGCY 7 cut(s) 164, 260, 272, 302, 389, 452, 473
CviQI GTAC 1 cut(s) 508
DdeI CTNAG 4 cut(s) 64, 89, 231, 411
DpnI GATC 2 cut(s) 431, 639
DpnII GATC 2 cut(s) 429, 637
EaeI YGGCCR 1 cut(s) 450
EagI CGGCCG 1 cut(s) 450
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
EclXI CGGCCG 1 cut(s) 450
Eco31I GGTCTC 1 cut(s) 328
Eco52I CGGCCG 1 cut(s) 450
Eco57I CTGAAG 2 cut(s) 186, 459
EcoRII CCWGG 1 cut(s) 292
FaeI CATG 2 cut(s) 155, 596
FaiI YATR 9 cut(s) 76, 117, 153, 332, 350, 392, 446, 561, 594
FatI CATG 2 cut(s) 151, 592
Fnu4HI GCNGC 4 cut(s) 242, 288, 450, 602
Fsp4HI GCNGC 4 cut(s) 242, 288, 450, 602
FspBI CTAG 2 cut(s) 530, 655
GlaI GCGC 2 cut(s) 240, 291
GluI GCNGC 4 cut(s) 242, 288, 450, 602
GsuI CTGGAG 1 cut(s) 144
HaeIII GGCC 2 cut(s) 260, 452
HapII CCGG 2 cut(s) 206, 585
HhaI GCGC 2 cut(s) 241, 292
Hin1II CATG 2 cut(s) 155, 596
Hin6I GCGC 2 cut(s) 239, 290
HinP1I GCGC 2 cut(s) 239, 290
HinfI GANTC 6 cut(s) 23, 155, 195, 211, 479, 550
HpaII CCGG 2 cut(s) 206, 585
HphI GGTGA 4 cut(s) 72, 135, 391, 571
Hpy188I TCNGA 7 cut(s) 67, 194, 216, 412, 478, 555, 614
Hpy188III TCNNGA 2 cut(s) 305, 631
HpyAV CCTTC 2 cut(s) 272, 360
HpyCH4V TGCA 1 cut(s) 601
HpyF10VI GCNNNNNNNGC 1 cut(s) 247
HpyF3I CTNAG 4 cut(s) 64, 89, 231, 411
Hsp92II CATG 2 cut(s) 155, 596
HspAI GCGC 2 cut(s) 239, 290
Kzo9I GATC 2 cut(s) 429, 637
LpnPI CCDG 9 cut(s) 174, 219, 279, 306, 449, 490, 531, 598, 626
Lsp1109I GCAGC 2 cut(s) 274, 613
LweI GCATC 1 cut(s) 69
MaeI CTAG 2 cut(s) 530, 655
MaeIII GTNAC 1 cut(s) 647
MalI GATC 2 cut(s) 431, 639
MboI GATC 2 cut(s) 429, 637
MboII GAAGA 3 cut(s) 59, 179, 367
MlyI GAGTC 1 cut(s) 189
MseI TTAA 1 cut(s) 401
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 164
MspI CCGG 2 cut(s) 206, 585
MspR9I CCNGG 1 cut(s) 294
MvaI CCWGG 1 cut(s) 294
MwoI GCNNNNNNNGC 1 cut(s) 247
NdeII GATC 2 cut(s) 429, 637
NlaIII CATG 2 cut(s) 155, 596
NmuCI GTSAC 1 cut(s) 647
NspI RCATGY 1 cut(s) 596
PciI ACATGT 1 cut(s) 592
PfeI GAWTC 5 cut(s) 23, 155, 211, 479, 550
PkrI GCNGC 4 cut(s) 243, 289, 451, 603
PleI GAGTC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 189
PscI ACATGT 1 cut(s) 592
Psp6I CCWGG 1 cut(s) 292
PspGI CCWGG 1 cut(s) 292
PspPI GGNCC 1 cut(s) 259
PstNI CAGNNNCTG 1 cut(s) 510
PvuII CAGCTG 1 cut(s) 164
RsaI GTAC 1 cut(s) 509
RsaNI GTAC 1 cut(s) 508
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 1 cut(s) 401
SatI GCNGC 4 cut(s) 242, 288, 450, 602
Sau3AI GATC 2 cut(s) 429, 637
Sau96I GGNCC 1 cut(s) 259
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 1 cut(s) 294
SetI ASST 4 cut(s) 85, 166, 509, 560
SfaNI GCATC 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 303
SmoI CTYRAG 1 cut(s) 303
SsiI CCGC 3 cut(s) 242, 248, 449
SspI AATATT 1 cut(s) 102
SspMI CTAG 2 cut(s) 530, 655
StyD4I CCNGG 1 cut(s) 292
TaqI TCGA 1 cut(s) 624
TauI GCSGC 2 cut(s) 244, 452
TfiI GAWTC 5 cut(s) 23, 155, 211, 479, 550
Tru1I TTAA 1 cut(s) 401
Tru9I TTAA 1 cut(s) 401
TscAI CASTG 1 cut(s) 525
TseFI GTSAC 1 cut(s) 647
TseI GCWGC 2 cut(s) 287, 601
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 525
XceI RCATGY 1 cut(s) 596
XspI CTAG 2 cut(s) 530, 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.