Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0009012

Physical Location & Seq

Genomic Coordinates Reverse (-)

2089275 .. 2091978

Gene Structure 2704 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1034100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 939 bp

ATGGAAACAATTCCAGAAGACCAACCAGAAACATCACCACAACGCAGAGAAGAAGAAAAACACAATGAAAAACAACAAGTGAAAGAGGAGGAGGAGGAAGAAGAAGAAGAAGAAAAGCAGAGCACCCCAGAAACACCATCTCCTCTGTTCCACTCACCGTCTCATGTGTTCCAATCACCATCTCATGTGTTCCAAACTCCGTCTCCACCGTCCGATTCTCCAATAACCCATCATACCTACCACACTCATGAGCTCAGAGTCGCGCCTGCAGACACCAACCCGGCGTCGCCGCCGCTCGCGCCGCCCCAGTCCTTCAGGGCCGAGCCCAAGGTTGCGGGACCCGATTCCAGAGATGGGCTTGATCGGACCCCGGAGGTTCGGGAAAAAAGTGGCGGTGGACTTGTCGGTGGTTCGGGTGTAAACATGCGAACTCTGAGACCCAAGTTGTCGATACTGAAGAGGTCGAGGAGGGAGAGCATGGTGAGGAGGGCTTTGTTTGGGTTAAGGATTTCTGGGTTTGTGTTGTGTTTGATATCTTTCTCGGTTATGGCGGCTGACAAGAACCAAGGTTGGGCTTTAGATTCCTTCTATCGCTACAAGGAGTTCAGGTTCTGCTTGGCAGTGAATGTGATTGGATTTGTGTATTCAGGGCTGCAAACGTATGACTTAACCTATTTCTTCACCAGCGGAAAACATGTAATCCAGCACCATCTCCGCTATTACTTTGATTTCTTGTTGGATCAGATTTTGACGTACCTTTTGATGTCGGCGTCTTCATCGGCTGCGATTCGGGTCGACGACTGGGAGTCGAATTGGGGCAAAGACAAGTTCCCGGACATGGCTCGGGCATCCGTCGCATTGTCCTTCCTTGCCTTTATTGCCTTTGCCTCCAGCTCCCTCATCTCTGGTTACACCCTTTGTACTCTGAAATCCATGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF588

Protein Analysis

313

Amino Acids

35.24

Weight (kDa)

6.02

Isoelectric Point (pI)

71.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CASP_dom	PF04535	160 - 294	1.7e-31	Casparian strip membrane protein domain
MARVEL	PF01284	165 - 298	3.3e-09	Membrane-associating domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009012)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G40300
fragaria_vesca	FvH4_6g50680 FvH4_6g50680 FvH4_6g50680 FvH4_6g50680
malus_domestica	MD09G1034100.v1.1
prunus_persica	Prupe.3G284200_v2.0.a1
pyrus_communis	pycom111g02710 pycom17g03190
rosa_chinensis	RchiOBHm_Chr2g0171451 RchiOBHm_Chr2g0171471
rosa_laevigata	RLG00000022015
rosa_multiflora	Rmu_sc0006783.1_g000001
rosa_roxburghii	Rroxscaffold_2G00080640
rosa_rugosa	Rorug02G0554500
rosa_samantha	Rh2AG628700 Rh2BG639100 Rh2BG639400 Rh2CG607800 Rh2DG650200 Rh2DG656200
rosa_wichuraiana	Rw2G052080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	295
AccI	GTMKAC	1 cut(s)	795
AccII	CGCG	2 cut(s)	263, 299
AciI	CCGC	8 cut(s)	290, 293, 302, 335, 393, 551, 687, 715
AclWI	GGATC	1 cut(s)	747
AcuI	CTGAAG	2 cut(s)	298, 476
AcyI	GRCGYC	2 cut(s)	284, 770
AfaI	GTAC	2 cut(s)	755, 922
AfiI	CCNNNNNNNGG	3 cut(s)	354, 571, 838
AflIII	ACRYGT	1 cut(s)	694
AhdI	GACNNNNNGTC	1 cut(s)	805
AloI	GAACNNNNNNTCC	2 cut(s)	593, 625
AluBI	AGCT	2 cut(s)	253, 894
AluI	AGCT	2 cut(s)	253, 894
Alw21I	GWGCWC	2 cut(s)	125, 255
Alw26I	GTCTC	3 cut(s)	165, 207, 430
AlwI	GGATC	1 cut(s)	747
AlwNI	CAGNNNCTG	1 cut(s)	612
Ama87I	CYCGRG	1 cut(s)	843
AoxI	GGCC	1 cut(s)	318
ApeKI	GCWGC	2 cut(s)	652, 782
ArsI	GACNNNNNNTTYG	2 cut(s)	742, 774
Asp700I	GAANNNNTTC	1 cut(s)	9
AspLEI	GCGC	2 cut(s)	265, 301
AspS9I	GGNCC	3 cut(s)	318, 338, 366
AsuC2I	CCSGG	3 cut(s)	281, 371, 833
AsuHPI	GGTGA	5 cut(s)	27, 147, 168, 493, 673
AvaI	CYCGRG	1 cut(s)	843
AvaII	GGWCC	2 cut(s)	338, 366
BanII	GRGCYC	2 cut(s)	255, 327
BbsI	GAAGAC	2 cut(s)	24, 765
Bbv12I	GWGCWC	2 cut(s)	125, 255
BbvI	GCAGC	2 cut(s)	639, 769
BccI	CCATC	5 cut(s)	145, 187, 237, 347, 717
BcnI	CCSGG	3 cut(s)	281, 371, 833
BcoDI	GTCTC	3 cut(s)	165, 207, 430
BfmI	CTRYAG	1 cut(s)	267
BisI	GCNGC	6 cut(s)	290, 293, 302, 552, 653, 783
BlsI	GCNGC	6 cut(s)	291, 294, 303, 553, 654, 784
Bme1390I	CCNGG	3 cut(s)	281, 371, 833
Bme18I	GGWCC	2 cut(s)	338, 366
BmeRI	GACNNNNNGTC	1 cut(s)	805
BmeT110I	CYCGRG	1 cut(s)	843
BmgT120I	GGNCC	3 cut(s)	318, 338, 366
BmiI	GGNNCC	3 cut(s)	339, 340, 368
BmrFI	CCNGG	3 cut(s)	281, 371, 833
BmrI	ACTGGG	2 cut(s)	301, 811
BmsI	GCATC	1 cut(s)	857
BmuI	ACTGGG	2 cut(s)	301, 811
BpiI	GAAGAC	2 cut(s)	24, 765
BpmI	CTGGAG	1 cut(s)	874
BpuMI	CCSGG	3 cut(s)	281, 371, 833
BsaHI	GRCGYC	2 cut(s)	284, 770
BsaI	GGTCTC	1 cut(s)	430
BsaJI	CCNNGG	3 cut(s)	327, 369, 565
BsaXI	ACNNNNNCTCC	6 cut(s)	124, 154, 187, 217, 593, 623
Bsc4I	CCNNNNNNNGG	3 cut(s)	354, 571, 838
Bse1I	ACTGG	2 cut(s)	307, 806
BseDI	CCNNGG	3 cut(s)	327, 369, 565
BseGI	GGATG	1 cut(s)	848
BseLI	CCNNNNNNNGG	3 cut(s)	354, 571, 838
BseMII	CTCAG	2 cut(s)	268, 425
BseNI	ACTGG	2 cut(s)	307, 806
BseRI	GAGGAG	6 cut(s)	101, 104, 107, 132, 481, 499
BseXI	GCAGC	2 cut(s)	639, 769
Bsh1236I	CGCG	2 cut(s)	263, 299
BshFI	GGCC	1 cut(s)	320
BsiHKAI	GWGCWC	2 cut(s)	125, 255
BsiHKCI	CYCGRG	1 cut(s)	843
BsiSI	CCGG	3 cut(s)	281, 371, 833
BslFI	GGGAC	1 cut(s)	351
BslI	CCNNNNNNNGG	3 cut(s)	354, 571, 838
BsmAI	GTCTC	3 cut(s)	165, 207, 430
BsmBI	CGTCTC	2 cut(s)	165, 207
BsmFI	GGGAC	1 cut(s)	351
BsnI	GGCC	1 cut(s)	320
Bso31I	GGTCTC	1 cut(s)	430
BsoBI	CYCGRG	1 cut(s)	843
Bsp1286I	GDGCHC	3 cut(s)	125, 255, 327
Bsp143I	GATC	2 cut(s)	361, 739
BspACI	CCGC	8 cut(s)	290, 293, 302, 335, 393, 551, 687, 715
BspANI	GGCC	1 cut(s)	320
BspCNI	CTCAG	2 cut(s)	267, 426
BspFNI	CGCG	2 cut(s)	263, 299
BspHI	TCATGA	1 cut(s)	247
BspLI	GGNNCC	3 cut(s)	339, 340, 368
BspMAI	CTGCAG	1 cut(s)	271
BspPI	GGATC	1 cut(s)	747
BspTNI	GGTCTC	1 cut(s)	430
BsrBI	CCGCTC	1 cut(s)	295
BsrI	ACTGG	2 cut(s)	307, 806
BssECI	CCNNGG	3 cut(s)	327, 369, 565
BssMI	GATC	2 cut(s)	361, 739
BssNI	GRCGYC	2 cut(s)	284, 770
BssT1I	CCWWGG	2 cut(s)	327, 565
Bst4CI	ACNGT	2 cut(s)	159, 210
Bst6I	CTCTTC	1 cut(s)	452
BstACI	GRCGYC	2 cut(s)	284, 770
BstC8I	GCNNGC	2 cut(s)	267, 297
BstDEI	CTNAG	2 cut(s)	254, 434
BstF5I	GGATG	1 cut(s)	848
BstFNI	CGCG	2 cut(s)	263, 299
BstHHI	GCGC	2 cut(s)	265, 301
BstKTI	GATC	2 cut(s)	364, 742
BstMAI	GTCTC	3 cut(s)	165, 207, 430
BstMBI	GATC	2 cut(s)	361, 739
BstMWI	GCNNNNNNNGC	4 cut(s)	298, 301, 854, 878
BstNSI	RCATGY	2 cut(s)	427, 698
BstSCI	CCNGG	3 cut(s)	279, 369, 831
BstSFI	CTRYAG	1 cut(s)	267
BstUI	CGCG	2 cut(s)	263, 299
BstV1I	GCAGC	2 cut(s)	639, 769
BstV2I	GAAGAC	2 cut(s)	24, 765
BsuRI	GGCC	1 cut(s)	320
BtsCI	GGATG	1 cut(s)	848
BtsI	GCAGTG	1 cut(s)	627
BtsIMutI	CAGTG	1 cut(s)	627
Cac8I	GCNNGC	2 cut(s)	267, 297
CaiI	CAGNNNCTG	1 cut(s)	612
CciI	TCATGA	1 cut(s)	247
CfoI	GCGC	2 cut(s)	265, 301
Cfr13I	GGNCC	3 cut(s)	318, 338, 366
CseI	GACGC	2 cut(s)	273, 759
Csp6I	GTAC	2 cut(s)	754, 921
CviAII	CATG	8 cut(s)	164, 185, 248, 424, 478, 695, 838, 934
CviQI	GTAC	2 cut(s)	754, 921
DdeI	CTNAG	2 cut(s)	254, 434
DpnI	GATC	2 cut(s)	363, 741
DpnII	GATC	2 cut(s)	361, 739
DriI	GACNNNNNGTC	1 cut(s)	805
Eam1104I	CTCTTC	1 cut(s)	452
Eam1105I	GACNNNNNGTC	1 cut(s)	805
EarI	CTCTTC	1 cut(s)	452
Ecl136II	GAGCTC	1 cut(s)	253
Eco130I	CCWWGG	2 cut(s)	327, 565
Eco24I	GRGCYC	2 cut(s)	255, 327
Eco31I	GGTCTC	1 cut(s)	430
Eco32I	GATATC	1 cut(s)	534
Eco47I	GGWCC	2 cut(s)	338, 366
Eco53kI	GAGCTC	1 cut(s)	253
Eco57I	CTGAAG	2 cut(s)	298, 476
Eco88I	CYCGRG	1 cut(s)	843
EcoICRI	GAGCTC	1 cut(s)	253
EcoO109I	RGGNCCY	1 cut(s)	338
EcoRV	GATATC	1 cut(s)	534
EcoT14I	CCWWGG	2 cut(s)	327, 565
EcoT38I	GRGCYC	2 cut(s)	255, 327
ErhI	CCWWGG	2 cut(s)	327, 565
Esp3I	CGTCTC	2 cut(s)	165, 207
FaeI	CATG	8 cut(s)	167, 188, 251, 427, 481, 698, 841, 937
FaqI	GGGAC	1 cut(s)	351
FatI	CATG	8 cut(s)	163, 184, 247, 423, 477, 694, 837, 933
FauI	CCCGC	1 cut(s)	328
FblI	GTMKAC	1 cut(s)	795
Fnu4HI	GCNGC	6 cut(s)	290, 293, 302, 552, 653, 783
FokI	GGATG	1 cut(s)	835
FriOI	GRGCYC	2 cut(s)	255, 327
Fsp4HI	GCNGC	6 cut(s)	290, 293, 302, 552, 653, 783
GlaI	GCGC	2 cut(s)	264, 300
GluI	GCNGC	6 cut(s)	290, 293, 302, 552, 653, 783
GsuI	CTGGAG	1 cut(s)	874
HaeIII	GGCC	1 cut(s)	320
HapII	CCGG	3 cut(s)	281, 371, 833
HgaI	GACGC	2 cut(s)	273, 759
HhaI	GCGC	2 cut(s)	265, 301
Hin1I	GRCGYC	2 cut(s)	284, 770
Hin1II	CATG	8 cut(s)	167, 188, 251, 427, 481, 698, 841, 937
Hin6I	GCGC	2 cut(s)	263, 299
HinP1I	GCGC	2 cut(s)	263, 299
HincII	GTYRAC	1 cut(s)	796
HindII	GTYRAC	1 cut(s)	796
HinfI	GANTC	6 cut(s)	215, 258, 344, 581, 787, 806
HpaII	CCGG	3 cut(s)	281, 371, 833
HphI	GGTGA	5 cut(s)	27, 147, 168, 493, 673
Hpy166II	GTNNAC	3 cut(s)	398, 421, 796
Hpy188I	TCNGA	6 cut(s)	214, 257, 366, 435, 744, 927
Hpy188III	TCNNGA	4 cut(s)	14, 248, 348, 380
Hpy8I	GTNNAC	3 cut(s)	398, 421, 796
Hpy99I	CGWCG	3 cut(s)	289, 800, 857
HpyAV	CCTTC	3 cut(s)	322, 595, 874
HpyCH4III	ACNGT	2 cut(s)	159, 210
HpyCH4IV	ACGT	2 cut(s)	659, 752
HpyCH4V	TGCA	2 cut(s)	269, 655
HpyF10VI	GCNNNNNNNGC	4 cut(s)	298, 301, 854, 878
HpyF3I	CTNAG	2 cut(s)	254, 434
HpySE526I	ACGT	2 cut(s)	659, 752
Hsp92I	GRCGYC	2 cut(s)	284, 770
Hsp92II	CATG	8 cut(s)	167, 188, 251, 427, 481, 698, 841, 937
HspAI	GCGC	2 cut(s)	263, 299
KflI	GGGWCCC	1 cut(s)	338
Kzo9I	GATC	2 cut(s)	361, 739
LmnI	GCTCC	1 cut(s)	899
Lsp1109I	GCAGC	2 cut(s)	639, 769
LweI	GCATC	1 cut(s)	857
MaeII	ACGT	2 cut(s)	659, 752
MaeIII	GTNAC	1 cut(s)	908
MalI	GATC	2 cut(s)	363, 741
MbiI	CCGCTC	1 cut(s)	295
MboI	GATC	2 cut(s)	361, 739
MhlI	GDGCHC	3 cut(s)	125, 255, 327
MluCI	AATT	2 cut(s)	9, 811
MlyI	GAGTC	2 cut(s)	267, 815
MmeI	TCCRAC	1 cut(s)	717
MroXI	GAANNNNTTC	1 cut(s)	9
MseI	TTAA	2 cut(s)	503, 668
MslI	CAYNNNNRTG	1 cut(s)	246
MspA1I	CMGCKG	1 cut(s)	687
MspI	CCGG	3 cut(s)	281, 371, 833
MspR9I	CCNGG	3 cut(s)	281, 371, 833
MvnI	CGCG	2 cut(s)	263, 299
MwoI	GCNNNNNNNGC	4 cut(s)	298, 301, 854, 878
NciI	CCSGG	3 cut(s)	281, 371, 833
NdeII	GATC	2 cut(s)	361, 739
NlaIII	CATG	8 cut(s)	167, 188, 251, 427, 481, 698, 841, 937
NlaIV	GGNNCC	3 cut(s)	339, 340, 368
NmeAIII	GCCGAG	1 cut(s)	346
NspI	RCATGY	2 cut(s)	427, 698
PagI	TCATGA	1 cut(s)	247
PciI	ACATGT	1 cut(s)	694
PdmI	GAANNNNTTC	1 cut(s)	9
PfeI	GAWTC	4 cut(s)	215, 344, 581, 787
PfoI	TCCNGGA	1 cut(s)	831
PkrI	GCNGC	6 cut(s)	291, 294, 303, 553, 654, 784
PleI	GAGTC	2 cut(s)	266, 814
PpsI	GAGTC	2 cut(s)	266, 814
PpuMI	RGGWCCY	1 cut(s)	338
PscI	ACATGT	1 cut(s)	694
Psp124BI	GAGCTC	1 cut(s)	255
Psp5II	RGGWCCY	1 cut(s)	338
PspN4I	GGNNCC	3 cut(s)	339, 340, 368
PspPI	GGNCC	3 cut(s)	318, 338, 366
PspPPI	RGGWCCY	1 cut(s)	338
PstI	CTGCAG	1 cut(s)	271
PstNI	CAGNNNCTG	1 cut(s)	612
RsaI	GTAC	2 cut(s)	755, 922
RsaNI	GTAC	2 cut(s)	754, 921
RseI	CAYNNNNRTG	1 cut(s)	246
SacI	GAGCTC	1 cut(s)	255
SalI	GTCGAC	1 cut(s)	794
SaqAI	TTAA	2 cut(s)	503, 668
SatI	GCNGC	6 cut(s)	290, 293, 302, 552, 653, 783
Sau3AI	GATC	2 cut(s)	361, 739
Sau96I	GGNCC	3 cut(s)	318, 338, 366
SchI	GAGTC	2 cut(s)	267, 815
ScrFI	CCNGG	3 cut(s)	281, 371, 833
SduI	GDGCHC	3 cut(s)	125, 255, 327
SfaNI	GCATC	1 cut(s)	857
SfcI	CTRYAG	1 cut(s)	267
SinI	GGWCC	2 cut(s)	338, 366
SmiMI	CAYNNNNRTG	1 cut(s)	246
Sse9I	AATT	2 cut(s)	9, 811
SsiI	CCGC	8 cut(s)	290, 293, 302, 335, 393, 551, 687, 715
SstI	GAGCTC	1 cut(s)	255
StyD4I	CCNGG	3 cut(s)	279, 369, 831
StyI	CCWWGG	2 cut(s)	327, 565
TaaI	ACNGT	2 cut(s)	159, 210
TaiI	ACGT	2 cut(s)	662, 755
TaqI	TCGA	4 cut(s)	449, 464, 795, 809
TasI	AATT	2 cut(s)	9, 811
TatI	WGTACW	1 cut(s)	920
TauI	GCSGC	4 cut(s)	292, 295, 304, 554
TfiI	GAWTC	4 cut(s)	215, 344, 581, 787
Tru1I	TTAA	2 cut(s)	503, 668
Tru9I	TTAA	2 cut(s)	503, 668
TscAI	CASTG	1 cut(s)	627
TseI	GCWGC	2 cut(s)	652, 782
TspDTI	ATGAA	2 cut(s)	81, 765
TspGWI	ACGGA	2 cut(s)	189, 841
TspRI	CASTG	1 cut(s)	627
VpaK11BI	GGWCC	2 cut(s)	338, 366
XceI	RCATGY	2 cut(s)	427, 698
XmiI	GTMKAC	1 cut(s)	795
XmnI	GAANNNNTTC	1 cut(s)	9

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.