Rh2BG639100

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009012
Physical Location & Seq
Forward (+)
85900851 .. 85903817
2967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG639100.1

Sequence Viewer

Length: 663 bp
ATGAAAAGAGAAACCCAACAAGAATCATCAAAAGACAAAGATGGAGAAGAAAAGCAAGGCATCTCAGAAAAGTCATATTCACCTCCTCTCAGTCCTCTAATATTCCCCCAAAACCATAACAAGTCCCCATCTCCATCTCCGTCTCCATCACCACTACATGATTCTCCAGCTGAAGAAATCTACCACACCAATGAGTTCAGACTCACACCACCGGAGAATCAGACCCCCCACCACTCTAAGTCTGCGCCGCCACCGCCCGTGGAGGCCCAAACCAAAGCCCAAGAGGGGTTTGCTGCGCCAGGAGAGGCTCAAGAGGGAGGAGAGAATAGGAGAACTATGAGACCCTATTTGTCTATATTGAAGAGGGCGAGAAGGCAAAGCGTGGTGAGGAGGGCTATGTTGGGGTTAAGGATTTCTGAGTTTTTGTTTTGTTTGATCTCTTTCTCGGTTATGGCGGCCGACAAGAAGCAGGGTTGGGCTTCAGATTCTTTCTCTAACTACAAGGAGTTCAGGTACTGCTTGGCAGTGAATGTGCTAGGGTTTGTGTATTCAGGGATTCAGACCTATGATTTAGTCTATTACTTCACCACCGGAAAACATGTAGTGCAGCACCATCTCCGATATTACTTCGATTTCTTGATGGATCAGGCAAGTCACAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0048037 GO:0051536 GO:0051540 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

220

Amino Acids

25.07

Weight (kDa)

8.44

Isoelectric Point (pI)

73.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 128 - 217 1.1e-19 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 248, 254, 455
AclWI GGATC 1 cut(s) 651
AcoI YGGCCR 1 cut(s) 456
AcuI CTGAAG 2 cut(s) 192, 465
AfaI GTAC 1 cut(s) 515
AfiI CCNNNNNNNGG 1 cut(s) 285
AflIII ACRYGT 1 cut(s) 598
AgsI TTSAA 1 cut(s) 361
AjnI CCWGG 1 cut(s) 298
AluBI AGCT 1 cut(s) 170
AluI AGCT 1 cut(s) 170
Alw26I GTCTC 2 cut(s) 147, 334
AlwI GGATC 1 cut(s) 651
AlwNI CAGNNNCTG 1 cut(s) 516
AoxI GGCC 2 cut(s) 264, 456
ApeKI GCWGC 2 cut(s) 293, 607
AspLEI GCGC 2 cut(s) 247, 298
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 4 cut(s) 72, 141, 397, 577
BbvI GCAGC 2 cut(s) 280, 619
BccI CCATC 6 cut(s) 35, 136, 142, 154, 621, 634
BciT130I CCWGG 1 cut(s) 300
BcoDI GTCTC 2 cut(s) 147, 334
BfaI CTAG 2 cut(s) 536, 661
BisI GCNGC 4 cut(s) 248, 294, 456, 608
BlsI GCNGC 4 cut(s) 249, 295, 457, 609
Bme1390I CCNGG 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 265
BmrFI CCNGG 1 cut(s) 300
BmsI GCATC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 294
BsaI GGTCTC 1 cut(s) 334
BsaJI CCNNGG 1 cut(s) 258
BsaWI WCCGGW 2 cut(s) 211, 590
BsaXI ACNNNNNCTCC 2 cut(s) 497, 527
Bsc4I CCNNNNNNNGG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 300
BseDI CCNNGG 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 285
BseMII CTCAG 3 cut(s) 78, 103, 408
BseRI GAGGAG 3 cut(s) 75, 333, 403
BseX3I CGGCCG 1 cut(s) 456
BseXI GCAGC 2 cut(s) 280, 619
BsgI GTGCAG 1 cut(s) 626
Bsh1285I CGRYCG 1 cut(s) 459
BshFI GGCC 2 cut(s) 266, 458
BsiEI CGRYCG 1 cut(s) 459
BsiSI CCGG 2 cut(s) 212, 591
BslFI GGGAC 1 cut(s) 109
BslI CCNNNNNNNGG 1 cut(s) 285
BsmAI GTCTC 2 cut(s) 147, 334
BsmBI CGTCTC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 109
BsnI GGCC 2 cut(s) 266, 458
Bso31I GGTCTC 1 cut(s) 334
Bsp143I GATC 2 cut(s) 435, 643
BspACI CCGC 3 cut(s) 248, 254, 455
BspANI GGCC 2 cut(s) 266, 458
BspCNI CTCAG 3 cut(s) 77, 102, 409
BspPI GGATC 1 cut(s) 651
BspTNI GGTCTC 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 258
BssMI GATC 2 cut(s) 435, 643
Bst2UI CCWGG 1 cut(s) 300
Bst6I CTCTTC 1 cut(s) 356
BstDEI CTNAG 4 cut(s) 64, 89, 237, 417
BstDSI CCRYGG 1 cut(s) 258
BstHHI GCGC 2 cut(s) 247, 298
BstKTI GATC 2 cut(s) 438, 646
BstMAI GTCTC 2 cut(s) 147, 334
BstMBI GATC 2 cut(s) 435, 643
BstMCI CGRYCG 1 cut(s) 459
BstMWI GCNNNNNNNGC 1 cut(s) 253
BstNI CCWGG 1 cut(s) 300
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 1 cut(s) 298
BstV1I GCAGC 2 cut(s) 280, 619
BstZI CGGCCG 1 cut(s) 456
BsuRI GGCC 2 cut(s) 266, 458
BtgI CCRYGG 1 cut(s) 258
BtsI GCAGTG 1 cut(s) 531
BtsIMutI CAGTG 1 cut(s) 531
CaiI CAGNNNCTG 1 cut(s) 516
CfoI GCGC 2 cut(s) 247, 298
Cfr13I GGNCC 1 cut(s) 265
Csp6I GTAC 1 cut(s) 514
CviAII CATG 2 cut(s) 158, 599
CviJI RGCY 7 cut(s) 170, 266, 278, 308, 395, 458, 479
CviKI_1 RGCY 7 cut(s) 170, 266, 278, 308, 395, 458, 479
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 4 cut(s) 64, 89, 237, 417
DpnI GATC 2 cut(s) 437, 645
DpnII GATC 2 cut(s) 435, 643
EaeI YGGCCR 1 cut(s) 456
EagI CGGCCG 1 cut(s) 456
Eam1104I CTCTTC 1 cut(s) 356
EarI CTCTTC 1 cut(s) 356
EclXI CGGCCG 1 cut(s) 456
Eco31I GGTCTC 1 cut(s) 334
Eco52I CGGCCG 1 cut(s) 456
Eco57I CTGAAG 2 cut(s) 192, 465
EcoRII CCWGG 1 cut(s) 298
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 2 cut(s) 161, 602
FaiI YATR 9 cut(s) 76, 117, 159, 338, 356, 398, 452, 567, 600
FaqI GGGAC 1 cut(s) 109
FatI CATG 2 cut(s) 157, 598
Fnu4HI GCNGC 4 cut(s) 248, 294, 456, 608
Fsp4HI GCNGC 4 cut(s) 248, 294, 456, 608
FspBI CTAG 2 cut(s) 536, 661
GlaI GCGC 2 cut(s) 246, 297
GluI GCNGC 4 cut(s) 248, 294, 456, 608
GsuI CTGGAG 1 cut(s) 150
HaeIII GGCC 2 cut(s) 266, 458
HapII CCGG 2 cut(s) 212, 591
HhaI GCGC 2 cut(s) 247, 298
Hin1II CATG 2 cut(s) 161, 602
Hin6I GCGC 2 cut(s) 245, 296
HinP1I GCGC 2 cut(s) 245, 296
HinfI GANTC 6 cut(s) 23, 161, 201, 217, 485, 556
HpaII CCGG 2 cut(s) 212, 591
HphI GGTGA 4 cut(s) 72, 141, 397, 577
Hpy188I TCNGA 7 cut(s) 67, 200, 222, 418, 484, 561, 620
Hpy188III TCNNGA 2 cut(s) 311, 637
HpyAV CCTTC 1 cut(s) 366
HpyCH4V TGCA 1 cut(s) 607
HpyF10VI GCNNNNNNNGC 1 cut(s) 253
HpyF3I CTNAG 4 cut(s) 64, 89, 237, 417
Hsp92II CATG 2 cut(s) 161, 602
HspAI GCGC 2 cut(s) 245, 296
Kzo9I GATC 2 cut(s) 435, 643
LpnPI CCDG 9 cut(s) 180, 225, 285, 312, 455, 496, 537, 604, 632
Lsp1109I GCAGC 2 cut(s) 280, 619
LweI GCATC 1 cut(s) 69
MaeI CTAG 2 cut(s) 536, 661
MaeIII GTNAC 1 cut(s) 653
MalI GATC 2 cut(s) 437, 645
MboI GATC 2 cut(s) 435, 643
MboII GAAGA 3 cut(s) 59, 185, 373
MlyI GAGTC 1 cut(s) 195
MseI TTAA 1 cut(s) 407
MslI CAYNNNNRTG 1 cut(s) 189
MspA1I CMGCKG 1 cut(s) 170
MspI CCGG 2 cut(s) 212, 591
MspR9I CCNGG 1 cut(s) 300
MvaI CCWGG 1 cut(s) 300
MwoI GCNNNNNNNGC 1 cut(s) 253
NdeII GATC 2 cut(s) 435, 643
NlaIII CATG 2 cut(s) 161, 602
NmuCI GTSAC 1 cut(s) 653
NspI RCATGY 1 cut(s) 602
PciI ACATGT 1 cut(s) 598
PfeI GAWTC 5 cut(s) 23, 161, 217, 485, 556
PkrI GCNGC 4 cut(s) 249, 295, 457, 609
PleI GAGTC 1 cut(s) 195
PpsI GAGTC 1 cut(s) 195
PscI ACATGT 1 cut(s) 598
Psp6I CCWGG 1 cut(s) 298
PspGI CCWGG 1 cut(s) 298
PspPI GGNCC 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 516
PvuII CAGCTG 1 cut(s) 170
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
RseI CAYNNNNRTG 1 cut(s) 189
SaqAI TTAA 1 cut(s) 407
SatI GCNGC 4 cut(s) 248, 294, 456, 608
Sau3AI GATC 2 cut(s) 435, 643
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 1 cut(s) 195
ScrFI CCNGG 1 cut(s) 300
SetI ASST 4 cut(s) 85, 172, 515, 566
SfaNI GCATC 1 cut(s) 69
SmiMI CAYNNNNRTG 1 cut(s) 189
SmlI CTYRAG 1 cut(s) 309
SmoI CTYRAG 1 cut(s) 309
SsiI CCGC 3 cut(s) 248, 254, 455
SspI AATATT 1 cut(s) 102
SspMI CTAG 2 cut(s) 536, 661
StyD4I CCNGG 1 cut(s) 298
TaqI TCGA 1 cut(s) 630
TauI GCSGC 2 cut(s) 250, 458
TfiI GAWTC 5 cut(s) 23, 161, 217, 485, 556
Tru1I TTAA 1 cut(s) 407
Tru9I TTAA 1 cut(s) 407
TscAI CASTG 1 cut(s) 531
TseFI GTSAC 1 cut(s) 653
TseI GCWGC 2 cut(s) 293, 607
Tsp45I GTSAC 1 cut(s) 653
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 129
TspRI CASTG 1 cut(s) 531
XceI RCATGY 1 cut(s) 602
XspI CTAG 2 cut(s) 536, 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.