Rh2BG639400

2-methylene-furan-3-one reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009012
Physical Location & Seq
Forward (+)
85909712 .. 85912609
2898 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG639400.1

Sequence Viewer

Length: 420 bp
ATGGCTGCAGCTCCAAGTGATTCCATACCCTCTGTATATGGGGCTTGGGTCTATTCAGAGTATGGAAAAACTGCAGATGTTCTGAAATTTGATCCAAATGTGGCTGTTCCTGAAATTAAAGAGGACCAGGTGCTGATCAAGGTAGTTGCTGCTTCTCTTAACCCAATTGATTTCATGAGAGCTCTTAGTTTCTTCAAGGACATAGAGGGTGATAGTCCCTTACCTATAATGACCTACCTTGTGATATCAGCATCTTCAGCAGCAGCGACTCGGGTTGAAGATTGGGAGTTGAATTGGGGCAAAGACAAGTTCCCTGATATGGCACGAGCATCGGTGGCACTATCCTTCCTTGCATTCTTTGCATTTGCCTCTAGCTCTCTCATCTCTGGTTACACCCTTTGTACTCTAAAATCTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N ADH_zinc_N_2

Protein Analysis

139

Amino Acids

15.12

Weight (kDa)

4.57

Isoelectric Point (pI)

27.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 76 - 121 8.7e-08 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 86
AcsI RAATTY 1 cut(s) 86
AcuI CTGAAG 1 cut(s) 240
AfaI GTAC 1 cut(s) 403
AfiI CCNNNNNNNGG 1 cut(s) 319
AgsI TTSAA 3 cut(s) 196, 278, 292
AjnI CCWGG 1 cut(s) 126
AluBI AGCT 3 cut(s) 11, 182, 375
AluI AGCT 3 cut(s) 11, 182, 375
Alw21I GWGCWC 1 cut(s) 184
AlwI GGATC 1 cut(s) 86
AlwNI CAGNNNCTG 1 cut(s) 133
Ama87I CYCGRG 1 cut(s) 270
ApeKI GCWGC 5 cut(s) 5, 8, 149, 260, 263
ApoI RAATTY 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 124
AsuHPI GGTGA 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 270
AvaII GGWCC 1 cut(s) 124
BanII GRGCYC 1 cut(s) 184
BauI CACGAG 1 cut(s) 324
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 4 cut(s) 20, 136, 272, 275
BcgI CGANNNNNNTGC 2 cut(s) 312, 346
BciT130I CCWGG 1 cut(s) 128
BclI TGATCA 1 cut(s) 135
BfaI CTAG 1 cut(s) 372
BfmI CTRYAG 2 cut(s) 6, 72
BisI GCNGC 5 cut(s) 6, 9, 150, 261, 264
BlsI GCNGC 5 cut(s) 7, 10, 151, 262, 265
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 270
BmgT120I GGNCC 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 2 cut(s) 260, 338
Bsc4I CCNNNNNNNGG 1 cut(s) 319
BseBI CCWGG 1 cut(s) 128
BseLI CCNNNNNNNGG 1 cut(s) 319
BseXI GCAGC 4 cut(s) 20, 136, 272, 275
BsiHKAI GWGCWC 1 cut(s) 184
BsiHKCI CYCGRG 1 cut(s) 270
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 353
BsoBI CYCGRG 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 2 cut(s) 91, 135
BspHI TCATGA 1 cut(s) 174
BspMAI CTGCAG 2 cut(s) 10, 76
BspPI GGATC 1 cut(s) 86
BssMI GATC 2 cut(s) 91, 135
BssSI CACGAG 1 cut(s) 324
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 128
BstAPI GCANNNNNTGC 1 cut(s) 359
BstDEI CTNAG 1 cut(s) 185
BstKTI GATC 2 cut(s) 94, 138
BstMBI GATC 2 cut(s) 91, 135
BstMWI GCNNNNNNNGC 3 cut(s) 257, 335, 359
BstNI CCWGG 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 126
BstSFI CTRYAG 2 cut(s) 6, 72
BstV1I GCAGC 4 cut(s) 20, 136, 272, 275
CaiI CAGNNNCTG 1 cut(s) 133
CciI TCATGA 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 124
CsiI ACCWGGT 1 cut(s) 126
Csp6I GTAC 1 cut(s) 402
CviAII CATG 1 cut(s) 175
CviJI RGCY 6 cut(s) 5, 11, 44, 104, 182, 375
CviKI_1 RGCY 6 cut(s) 5, 11, 44, 104, 182, 375
CviQI GTAC 1 cut(s) 402
DdeI CTNAG 1 cut(s) 185
DpnI GATC 2 cut(s) 93, 137
DpnII GATC 2 cut(s) 91, 135
Ecl136II GAGCTC 1 cut(s) 182
Eco24I GRGCYC 1 cut(s) 184
Eco32I GATATC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 124
Eco53kI GAGCTC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 240
Eco88I CYCGRG 1 cut(s) 270
EcoICRI GAGCTC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 126
EcoRV GATATC 1 cut(s) 246
EcoT38I GRGCYC 1 cut(s) 184
FaeI CATG 1 cut(s) 178
FaiI YATR 9 cut(s) 26, 37, 39, 63, 176, 203, 227, 320, 416
FaqI GGGAC 1 cut(s) 201
FatI CATG 1 cut(s) 174
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 5 cut(s) 6, 9, 150, 261, 264
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 5 cut(s) 6, 9, 150, 261, 264
FspBI CTAG 1 cut(s) 372
GluI GCNGC 5 cut(s) 6, 9, 150, 261, 264
Hin1II CATG 1 cut(s) 178
HinfI GANTC 2 cut(s) 20, 268
HphI GGTGA 1 cut(s) 221
Hpy188I TCNGA 2 cut(s) 58, 84
Hpy188III TCNNGA 2 cut(s) 110, 175
HpyAV CCTTC 1 cut(s) 355
HpyCH4V TGCA 4 cut(s) 8, 74, 353, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 335, 359
HpyF3I CTNAG 1 cut(s) 185
Hsp92II CATG 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 2 cut(s) 91, 135
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 5 cut(s) 113, 123, 140, 327, 372
Lsp1109I GCAGC 4 cut(s) 20, 136, 272, 275
LweI GCATC 2 cut(s) 260, 338
MabI ACCWGGT 1 cut(s) 126
MaeI CTAG 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 389
MalI GATC 2 cut(s) 93, 137
MboI GATC 2 cut(s) 91, 135
MboII GAAGA 3 cut(s) 184, 246, 290
MfeI CAATTG 1 cut(s) 165
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 4 cut(s) 86, 114, 165, 292
MlyI GAGTC 1 cut(s) 262
MnlI CCTC 4 cut(s) 40, 115, 199, 379
MseI TTAA 2 cut(s) 117, 159
MspR9I CCNGG 1 cut(s) 128
MunI CAATTG 1 cut(s) 165
Mva1269I GAATGC 1 cut(s) 353
MvaI CCWGG 1 cut(s) 128
MwoI GCNNNNNNNGC 3 cut(s) 257, 335, 359
NdeII GATC 2 cut(s) 91, 135
NlaIII CATG 1 cut(s) 178
PagI TCATGA 1 cut(s) 174
PctI GAATGC 1 cut(s) 353
PfeI GAWTC 1 cut(s) 20
PkrI GCNGC 5 cut(s) 7, 10, 151, 262, 265
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Psp124BI GAGCTC 1 cut(s) 184
Psp6I CCWGG 1 cut(s) 126
PspGI CCWGG 1 cut(s) 126
PspPI GGNCC 1 cut(s) 124
PstI CTGCAG 2 cut(s) 10, 76
PstNI CAGNNNCTG 1 cut(s) 133
RsaI GTAC 1 cut(s) 403
RsaNI GTAC 1 cut(s) 402
SacI GAGCTC 1 cut(s) 184
SaqAI TTAA 2 cut(s) 117, 159
SatI GCNGC 5 cut(s) 6, 9, 150, 261, 264
Sau3AI GATC 2 cut(s) 91, 135
Sau96I GGNCC 1 cut(s) 124
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 1 cut(s) 128
SduI GDGCHC 1 cut(s) 184
SetI ASST 8 cut(s) 13, 132, 144, 184, 226, 236, 240, 377
SexAI ACCWGGT 1 cut(s) 126
SfaNI GCATC 2 cut(s) 260, 338
SfcI CTRYAG 2 cut(s) 6, 72
SinI GGWCC 1 cut(s) 124
Sse9I AATT 4 cut(s) 86, 114, 165, 292
SspMI CTAG 1 cut(s) 372
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 1 cut(s) 126
TasI AATT 4 cut(s) 86, 114, 165, 292
TatI WGTACW 1 cut(s) 401
TfiI GAWTC 1 cut(s) 20
Tru1I TTAA 2 cut(s) 117, 159
Tru9I TTAA 2 cut(s) 117, 159
TseI GCWGC 5 cut(s) 5, 8, 149, 260, 263
TspDTI ATGAA 1 cut(s) 163
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 1 cut(s) 86
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.