AT5G44520

Ribose 5-phosphate isomerase A (phosphoriboisomerase A)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0006749
Physical Location & Seq
Reverse (-)
17934287 .. 17936554
2268 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44520.1

Sequence Viewer

Length: 891 bp
ATGGTTACTGCGACAGCATCAACTCCATTTTCACTCTCGTCGTCCGTGGTTTTCACGCGCCGTCGTAATTTCAAACGTTTTCCAGTTTGCGCCAGTCTTTCTCCGGAGGTTTCTCCTCTTCTCAGAGCAGCTCATCACACTGTGGATAATTATGTAAAGAGTGGGATGATTATTGGTTTAGGATCTGGAGAAGCTTCAGATTTTGCTATACGTTATTTGGGTCAACAACTTGGTTCTGGTTCTTTACATAATGTTGTTGGTGTACCAATGTCTGCTCGAAGTGCGAGTGAAGCAGCAAAGTATGGAATCCCCTTGGAATATTACCGGGATGGTGTTCAGATTGATTTTGCATTTCATGATGCTGATGCTGTAGAAGAGAATACGCTTATTGCAGTTATTGGACGGCGGAGAAGTTCACAGGAAGATGACTATATTCTGAAACAAAAGTCTATTGTAAAAGTAGCTGATGAGGCAGTCTTCATGATAAAGGAAGAACAATACAAAGCCGGTCTTGAAGGTTCTATCCCTGTTTTAGTTCAATCTCTTAATTGGTTGGCCATAGCTGAGGAGATAGATGACTTGTATCTAGGCGATGCAGAGGTGTGGAGAAGAGCTTCTGTTGAAAATGAAGGCCCTCTTGGAGGAGACTTTCCTATAGTTACCAGTGATGGTCACAACATTCTTGATGTTATATTTACAACTCCGATTCGAAGCCTCGCTGACTTGGCTACAAGCCTTGATAAGATTGATGGGGTTGTGGACCATGGACTAATTATCAAAACTCGATGCACGGTGGTGATTGCAGAAGAAACTGTTGTAAGAAGTGTTACCTTGCAGACCAGTGCATTGGAGGGTGGTGTACGAACCTATGGACAGACTTTGCAAAAGTAG

Protein Analysis

296

Amino Acids

32.16

Weight (kDa)

5.13

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rib_5-P_isom_A PF06026 86 - 270 2.5e-33 Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 58
AccIII TCCGGA 1 cut(s) 103
AciI CCGC 1 cut(s) 406
AclI AACGTT 1 cut(s) 76
AclWI GGATC 1 cut(s) 190
AcoI YGGCCR 1 cut(s) 555
AcuI CTGAAG 1 cut(s) 180
AdeI CACNNNGTG 1 cut(s) 142
AfaI GTAC 2 cut(s) 264, 861
AfiI CCNNNNNNNGG 1 cut(s) 641
AgsI TTSAA 4 cut(s) 73, 515, 539, 623
AjuI GAANNNNNNNTTGG 2 cut(s) 621, 653
AleI CACNNNNGTG 1 cut(s) 794
AluBI AGCT 5 cut(s) 131, 194, 464, 563, 614
AluI AGCT 5 cut(s) 131, 194, 464, 563, 614
Alw26I GTCTC 1 cut(s) 639
AlwI GGATC 1 cut(s) 190
Aor13HI TCCGGA 1 cut(s) 103
AoxI GGCC 2 cut(s) 555, 631
ApeKI GCWGC 2 cut(s) 128, 293
Asp700I GAANNNNTTC 1 cut(s) 613
AspLEI GCGC 2 cut(s) 60, 92
AspS9I GGNCC 2 cut(s) 632, 760
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 1 cut(s) 808
AsuII TTCGAA 1 cut(s) 709
AvaII GGWCC 1 cut(s) 760
BalI TGGCCA 1 cut(s) 557
BbsI GAAGAC 1 cut(s) 469
BbvCI CCTCAGC 1 cut(s) 564
BbvI GCAGC 2 cut(s) 140, 305
BccI CCATC 3 cut(s) 323, 662, 743
BceAI ACGGC 2 cut(s) 45, 419
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 639
BfaI CTAG 1 cut(s) 587
BfmI CTRYAG 2 cut(s) 369, 654
BisI GCNGC 2 cut(s) 129, 294
BlsI GCNGC 2 cut(s) 130, 295
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 1 cut(s) 760
BmgT120I GGNCC 2 cut(s) 632, 760
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 5 cut(s) 26, 349, 355, 583, 776
BpiI GAAGAC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 207
Bpu10I CCTNAGC 1 cut(s) 564
Bpu14I TTCGAA 1 cut(s) 709
BpuMI CCSGG 1 cut(s) 326
BsaJI CCNNGG 3 cut(s) 45, 312, 763
BsaWI WCCGGW 1 cut(s) 103
Bsc4I CCNNNNNNNGG 1 cut(s) 641
Bse118I RCCGGY 1 cut(s) 506
Bse1I ACTGG 4 cut(s) 83, 93, 663, 840
BseAI TCCGGA 1 cut(s) 103
BseDI CCNNGG 3 cut(s) 45, 312, 763
BseGI GGATG 2 cut(s) 171, 334
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMII CTCAG 2 cut(s) 136, 555
BseNI ACTGG 4 cut(s) 83, 93, 663, 840
BseRI GAGGAG 3 cut(s) 105, 581, 657
BseXI GCAGC 2 cut(s) 140, 305
Bsh1236I CGCG 1 cut(s) 58
BshFI GGCC 2 cut(s) 557, 633
BsiSI CCGG 3 cut(s) 104, 325, 507
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 1 cut(s) 639
BsnI GGCC 2 cut(s) 557, 633
Bsp119I TTCGAA 1 cut(s) 709
Bsp13I TCCGGA 1 cut(s) 103
Bsp143I GATC 1 cut(s) 182
Bsp19I CCATGG 1 cut(s) 763
BspACI CCGC 1 cut(s) 406
BspANI GGCC 2 cut(s) 557, 633
BspCNI CTCAG 2 cut(s) 135, 556
BspEI TCCGGA 1 cut(s) 103
BspFNI CGCG 1 cut(s) 58
BspHI TCATGA 2 cut(s) 355, 480
BspPI GGATC 1 cut(s) 190
BspQI GCTCTTC 1 cut(s) 604
BspT104I TTCGAA 1 cut(s) 709
BsrFI RCCGGY 1 cut(s) 506
BsrI ACTGG 4 cut(s) 83, 93, 663, 840
BssAI RCCGGY 1 cut(s) 506
BssECI CCNNGG 3 cut(s) 45, 312, 763
BssMI GATC 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 312, 763
Bst4CI ACNGT 3 cut(s) 142, 793, 814
Bst6I CTCTTC 3 cut(s) 123, 369, 604
BstBI TTCGAA 1 cut(s) 709
BstDEI CTNAG 2 cut(s) 122, 564
BstDSI CCRYGG 2 cut(s) 45, 763
BstENI CCTNNNNNAGG 1 cut(s) 639
BstF5I GGATG 2 cut(s) 171, 334
BstFNI CGCG 1 cut(s) 58
BstHHI GCGC 2 cut(s) 60, 92
BstKTI GATC 1 cut(s) 185
BstMAI GTCTC 1 cut(s) 639
BstMBI GATC 1 cut(s) 182
BstMWI GCNNNNNNNGC 4 cut(s) 281, 290, 470, 725
BstSCI CCNGG 1 cut(s) 324
BstSFI CTRYAG 2 cut(s) 369, 654
BstUI CGCG 1 cut(s) 58
BstV1I GCAGC 2 cut(s) 140, 305
BstV2I GAAGAC 1 cut(s) 469
BstX2I RGATCY 1 cut(s) 182
BstXI CCANNNNNNTGG 1 cut(s) 847
BstYI RGATCY 1 cut(s) 182
BsuRI GGCC 2 cut(s) 557, 633
BtgI CCRYGG 2 cut(s) 45, 763
BtgZI GCGATG 1 cut(s) 606
BtsCI GGATG 2 cut(s) 171, 334
BtsIMutI CAGTG 3 cut(s) 138, 670, 847
CciI TCATGA 2 cut(s) 355, 480
CfoI GCGC 2 cut(s) 60, 92
Cfr10I RCCGGY 1 cut(s) 506
Cfr13I GGNCC 2 cut(s) 632, 760
Csp6I GTAC 2 cut(s) 263, 860
CviAII CATG 3 cut(s) 356, 481, 764
CviQI GTAC 2 cut(s) 263, 860
DdeI CTNAG 2 cut(s) 122, 564
DpnI GATC 1 cut(s) 184
DpnII GATC 1 cut(s) 182
DraIII CACNNNGTG 1 cut(s) 142
EaeI YGGCCR 1 cut(s) 555
Eam1104I CTCTTC 3 cut(s) 123, 369, 604
EarI CTCTTC 3 cut(s) 123, 369, 604
EciI GGCGGA 1 cut(s) 421
Eco130I CCWWGG 2 cut(s) 312, 763
Eco47I GGWCC 1 cut(s) 760
Eco57I CTGAAG 1 cut(s) 180
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoO109I RGGNCCY 1 cut(s) 632
EcoT14I CCWWGG 2 cut(s) 312, 763
ErhI CCWWGG 2 cut(s) 312, 763
FaeI CATG 3 cut(s) 359, 484, 767
FalI AAGNNNNNCTT 4 cut(s) 495, 527, 621, 653
FatI CATG 3 cut(s) 355, 480, 763
Fnu4HI GCNGC 2 cut(s) 129, 294
FokI GGATG 2 cut(s) 178, 341
Fsp4HI GCNGC 2 cut(s) 129, 294
FspBI CTAG 1 cut(s) 587
GlaI GCGC 2 cut(s) 59, 91
GluI GCNGC 2 cut(s) 129, 294
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 2 cut(s) 557, 633
HapII CCGG 3 cut(s) 104, 325, 507
HhaI GCGC 2 cut(s) 60, 92
Hin1II CATG 3 cut(s) 359, 484, 767
Hin6I GCGC 2 cut(s) 58, 90
HinP1I GCGC 2 cut(s) 58, 90
HincII GTYRAC 1 cut(s) 224
HindII GTYRAC 1 cut(s) 224
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 2 cut(s) 306, 706
HpaII CCGG 3 cut(s) 104, 325, 507
HphI GGTGA 1 cut(s) 808
Hpy166II GTNNAC 5 cut(s) 224, 263, 416, 760, 860
Hpy188I TCNGA 5 cut(s) 125, 199, 339, 438, 705
Hpy188III TCNNGA 6 cut(s) 104, 186, 356, 481, 512, 683
Hpy8I GTNNAC 5 cut(s) 224, 263, 416, 760, 860
Hpy99I CGWCG 2 cut(s) 43, 66
HpyAV CCTTC 2 cut(s) 509, 623
HpyCH4III ACNGT 3 cut(s) 142, 793, 814
HpyCH4IV ACGT 2 cut(s) 76, 211
HpyCH4V TGCA 8 cut(s) 350, 392, 596, 789, 803, 835, 845, 883
HpyF10VI GCNNNNNNNGC 4 cut(s) 281, 290, 470, 725
HpyF3I CTNAG 2 cut(s) 122, 564
HpySE526I ACGT 2 cut(s) 76, 211
Hsp92II CATG 3 cut(s) 359, 484, 767
HspAI GCGC 2 cut(s) 58, 90
Kpn2I TCCGGA 1 cut(s) 103
Kzo9I GATC 1 cut(s) 182
LguI GCTCTTC 1 cut(s) 604
Lsp1109I GCAGC 2 cut(s) 140, 305
LweI GCATC 5 cut(s) 26, 349, 355, 583, 776
MaeI CTAG 1 cut(s) 587
MaeII ACGT 2 cut(s) 76, 211
MaeIII GTNAC 4 cut(s) 4, 658, 671, 826
MalI GATC 1 cut(s) 184
MboI GATC 1 cut(s) 182
MboII GAAGA 7 cut(s) 110, 386, 434, 469, 503, 621, 818
MflI RGATCY 1 cut(s) 182
MlsI TGGCCA 1 cut(s) 557
MluCI AATT 4 cut(s) 67, 148, 547, 771
MluNI TGGCCA 1 cut(s) 557
MnlI CCTC 9 cut(s) 100, 126, 463, 559, 592, 635, 645, 725, 844
Mox20I TGGCCA 1 cut(s) 557
MroI TCCGGA 1 cut(s) 103
MroXI GAANNNNTTC 1 cut(s) 613
MscI TGGCCA 1 cut(s) 557
MseI TTAA 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 794
Msp20I TGGCCA 1 cut(s) 557
MspI CCGG 3 cut(s) 104, 325, 507
MspR9I CCNGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 58
MwoI GCNNNNNNNGC 4 cut(s) 281, 290, 470, 725
NciI CCSGG 1 cut(s) 326
NcoI CCATGG 1 cut(s) 763
NdeII GATC 1 cut(s) 182
NlaIII CATG 3 cut(s) 359, 484, 767
NmuCI GTSAC 1 cut(s) 671
NspV TTCGAA 1 cut(s) 709
OliI CACNNNNGTG 1 cut(s) 794
PagI TCATGA 2 cut(s) 355, 480
PciSI GCTCTTC 1 cut(s) 604
PdmI GAANNNNTTC 1 cut(s) 613
PfeI GAWTC 2 cut(s) 306, 706
PkrI GCNGC 2 cut(s) 130, 295
Psp1406I AACGTT 1 cut(s) 76
PspPI GGNCC 2 cut(s) 632, 760
PsuI RGATCY 1 cut(s) 182
RsaI GTAC 2 cut(s) 264, 861
RsaNI GTAC 2 cut(s) 263, 860
RseI CAYNNNNRTG 1 cut(s) 794
SapI GCTCTTC 1 cut(s) 604
SaqAI TTAA 1 cut(s) 546
SatI GCNGC 2 cut(s) 129, 294
Sau3AI GATC 1 cut(s) 182
Sau96I GGNCC 2 cut(s) 632, 760
ScrFI CCNGG 1 cut(s) 326
SfaNI GCATC 5 cut(s) 26, 349, 355, 583, 776
SfcI CTRYAG 2 cut(s) 369, 654
SfuI TTCGAA 1 cut(s) 709
SinI GGWCC 1 cut(s) 760
SmiMI CAYNNNNRTG 1 cut(s) 794
Sse9I AATT 4 cut(s) 67, 148, 547, 771
SsiI CCGC 1 cut(s) 406
SspI AATATT 1 cut(s) 320
SspMI CTAG 1 cut(s) 587
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 2 cut(s) 312, 763
TaaI ACNGT 3 cut(s) 142, 793, 814
TaiI ACGT 2 cut(s) 79, 214
TaqI TCGA 3 cut(s) 277, 709, 784
TasI AATT 4 cut(s) 67, 148, 547, 771
TfiI GAWTC 2 cut(s) 306, 706
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TscAI CASTG 3 cut(s) 145, 670, 847
TseFI GTSAC 1 cut(s) 671
TseI GCWGC 2 cut(s) 128, 293
Tsp45I GTSAC 1 cut(s) 671
TspDTI ATGAA 3 cut(s) 344, 469, 642
TspGWI ACGGA 1 cut(s) 34
TspRI CASTG 3 cut(s) 145, 670, 847
VpaK11BI GGWCC 1 cut(s) 760
XagI CCTNNNNNAGG 1 cut(s) 639
XmnI GAANNNNTTC 1 cut(s) 613
XspI CTAG 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.