Rh5DG136000

Ribose-5-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0006749
Physical Location & Seq
Forward (+)
13605943 .. 13608207
2265 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG136000.1

Sequence Viewer

Length: 597 bp
ATGGCACTGGCAATGGCAGCCATAGCAATAGCTTCACCAAATCCCCTGCATTCATCTCTAACTTTATCAAATGCCAGAGGCAGAAGAGCCTTCTGCACAAGAACAAGAACAAGAAGAAGAACCCATTCTCTGAAGATTACTGGGTCCAGTCTTGCTGATGGCTCTGCTCTTTTTCAAGCAGCCCATTACACTGTGGATACCTACATTAAAAGTGGTATGGTGATTGGGTTGGGGTCTGGTATAGCTTCTGGTATGGCCATGCAGTATATAGGTCTGCAACTTGGAGCAGGTGCTTTAAAGGATATAGTGGGGATACCGACGTCTGTAGCATCTGCAAGTGAGGCAGCAAAGGCAGGAATTCCATTGGGTCACCATGAGGATAGTTCTCAAATTGATTTTGCATTTGATGATGCTGATATTATAGAAGAAAGGACACTTATTGCTGTCATTGGGAGAACAAGATCGCAGGGTGAGGAGTCAATCATCCAAGAGAAGACTATATTAAACGCCGCCAATAAAGTTGTCTTCATAGTCAGAGAAAACCAGTATAAATGTGGTATGGATGGTTCTATTCCAGTTTTGGTGAATCCTGTATAA

Protein Analysis

198

Amino Acids

20.88

Weight (kDa)

8.62

Isoelectric Point (pI)

37.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rib_5-P_isom_A PF06026 103 - 197 4.6e-16 Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 278
AatII GACGTC 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 278
AciI CCGC 1 cut(s) 510
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 1 cut(s) 357
AcuI CTGAAG 1 cut(s) 152
AcyI GRCGYC 1 cut(s) 320
AgsI TTSAA 1 cut(s) 176
AluBI AGCT 2 cut(s) 32, 245
AluI AGCT 2 cut(s) 32, 245
AoxI GGCC 1 cut(s) 255
ApeKI GCWGC 3 cut(s) 17, 179, 344
ApoI RAATTY 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 124
AspS9I GGNCC 1 cut(s) 144
AsuHPI GGTGA 5 cut(s) 27, 232, 362, 482, 595
AvaII GGWCC 1 cut(s) 144
BaeI ACNNNNGTAYC 2 cut(s) 305, 338
BalI TGGCCA 1 cut(s) 257
BbsI GAAGAC 2 cut(s) 500, 517
BbvI GCAGC 3 cut(s) 29, 191, 356
BccI CCATC 2 cut(s) 152, 557
BciVI GTATCC 2 cut(s) 190, 306
BfmI CTRYAG 1 cut(s) 324
BfuAI ACCTGC 1 cut(s) 278
BfuI GTATCC 2 cut(s) 190, 306
BisI GCNGC 4 cut(s) 18, 180, 345, 510
BlsI GCNGC 4 cut(s) 19, 181, 346, 511
Bme18I GGWCC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 144
BmiI GGNNCC 1 cut(s) 145
BmrI ACTGGG 1 cut(s) 150
BmsI GCATC 2 cut(s) 338, 400
BmuI ACTGGG 1 cut(s) 150
BpiI GAAGAC 2 cut(s) 500, 517
BsaHI GRCGYC 1 cut(s) 320
Bse1I ACTGG 5 cut(s) 12, 145, 147, 544, 575
Bse3DI GCAATG 1 cut(s) 18
BseGI GGATG 2 cut(s) 483, 568
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 5 cut(s) 12, 145, 147, 544, 575
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 3 cut(s) 29, 191, 356
BsgI GTGCAG 1 cut(s) 79
BshFI GGCC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 49
BsnI GGCC 1 cut(s) 257
Bsp143I GATC 1 cut(s) 461
BspACI CCGC 1 cut(s) 510
BspANI GGCC 1 cut(s) 257
BspLI GGNNCC 1 cut(s) 145
BspMI ACCTGC 1 cut(s) 278
BspQI GCTCTTC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 5 cut(s) 12, 145, 147, 544, 575
BssMI GATC 1 cut(s) 461
BssNI GRCGYC 1 cut(s) 320
Bst4CI ACNGT 1 cut(s) 193
Bst6I CTCTTC 1 cut(s) 79
BstACI GRCGYC 1 cut(s) 320
BstEII GGTNACC 1 cut(s) 368
BstF5I GGATG 2 cut(s) 483, 568
BstKTI GATC 1 cut(s) 464
BstMBI GATC 1 cut(s) 461
BstMWI GCNNNNNNNGC 4 cut(s) 17, 23, 341, 350
BstPI GGTNACC 1 cut(s) 368
BstSFI CTRYAG 1 cut(s) 324
BstV1I GCAGC 3 cut(s) 29, 191, 356
BstV2I GAAGAC 2 cut(s) 500, 517
BsuI GTATCC 2 cut(s) 190, 306
BsuRI GGCC 1 cut(s) 257
BtsCI GGATG 2 cut(s) 483, 568
BtsIMutI CAGTG 2 cut(s) 5, 189
BveI ACCTGC 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 144
CviAII CATG 2 cut(s) 259, 374
CviJI RGCY 7 cut(s) 20, 32, 89, 162, 182, 245, 257
CviKI_1 RGCY 7 cut(s) 20, 32, 89, 162, 182, 245, 257
DpnI GATC 1 cut(s) 463
DpnII GATC 1 cut(s) 461
DraI TTTAAA 1 cut(s) 297
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 79
EarI CTCTTC 1 cut(s) 79
Eco47I GGWCC 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 152
Eco91I GGTNACC 1 cut(s) 368
EcoO65I GGTNACC 1 cut(s) 368
EcoRI GAATTC 1 cut(s) 357
FaeI CATG 2 cut(s) 262, 377
FatI CATG 2 cut(s) 258, 373
Fnu4HI GCNGC 4 cut(s) 18, 180, 345, 510
FokI GGATG 2 cut(s) 470, 575
Fsp4HI GCNGC 4 cut(s) 18, 180, 345, 510
GluI GCNGC 4 cut(s) 18, 180, 345, 510
HaeIII GGCC 1 cut(s) 257
Hin1I GRCGYC 1 cut(s) 320
Hin1II CATG 2 cut(s) 262, 377
HinfI GANTC 2 cut(s) 476, 586
HphI GGTGA 5 cut(s) 27, 232, 362, 482, 595
Hpy188I TCNGA 2 cut(s) 132, 536
Hpy99I CGWCG 1 cut(s) 322
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4IV ACGT 1 cut(s) 320
HpyCH4V TGCA 6 cut(s) 49, 96, 262, 277, 335, 401
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 23, 341, 350
HpySE526I ACGT 1 cut(s) 320
Hsp92I GRCGYC 1 cut(s) 320
Hsp92II CATG 2 cut(s) 262, 377
Kzo9I GATC 1 cut(s) 461
LguI GCTCTTC 1 cut(s) 79
LmnI GCTCC 1 cut(s) 284
Lsp1109I GCAGC 3 cut(s) 29, 191, 356
LweI GCATC 2 cut(s) 338, 400
MaeII ACGT 1 cut(s) 320
MaeIII GTNAC 1 cut(s) 368
MalI GATC 1 cut(s) 463
MboI GATC 1 cut(s) 461
MboII GAAGA 7 cut(s) 96, 126, 129, 145, 437, 505, 517
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 2 cut(s) 357, 390
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 1 cut(s) 485
MnlI CCTC 4 cut(s) 71, 334, 370, 466
Mox20I TGGCCA 1 cut(s) 257
MroXI GAANNNNTTC 1 cut(s) 124
MscI TGGCCA 1 cut(s) 257
MseI TTAA 3 cut(s) 207, 296, 503
Msp20I TGGCCA 1 cut(s) 257
Mva1269I GAATGC 1 cut(s) 49
MwoI GCNNNNNNNGC 4 cut(s) 17, 23, 341, 350
NdeII GATC 1 cut(s) 461
NlaIII CATG 2 cut(s) 262, 377
NlaIV GGNNCC 1 cut(s) 145
NmuCI GTSAC 1 cut(s) 368
PaqCI CACCTGC 1 cut(s) 278
PciSI GCTCTTC 1 cut(s) 79
PctI GAATGC 1 cut(s) 49
PdmI GAANNNNTTC 1 cut(s) 124
PfeI GAWTC 1 cut(s) 586
PkrI GCNGC 4 cut(s) 19, 181, 346, 511
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
PspEI GGTNACC 1 cut(s) 368
PspN4I GGNNCC 1 cut(s) 145
PspPI GGNCC 1 cut(s) 144
PsrI GAACNNNNNNTAC 2 cut(s) 550, 582
SapI GCTCTTC 1 cut(s) 79
SaqAI TTAA 3 cut(s) 207, 296, 503
SatI GCNGC 4 cut(s) 18, 180, 345, 510
Sau3AI GATC 1 cut(s) 461
Sau96I GGNCC 1 cut(s) 144
SchI GAGTC 1 cut(s) 485
SetI ASST 6 cut(s) 34, 203, 247, 274, 292, 323
SfaNI GCATC 2 cut(s) 338, 400
SfcI CTRYAG 1 cut(s) 324
SinI GGWCC 1 cut(s) 144
Sse9I AATT 2 cut(s) 357, 390
SsiI CCGC 1 cut(s) 510
TaaI ACNGT 1 cut(s) 193
TaiI ACGT 1 cut(s) 323
TasI AATT 2 cut(s) 357, 390
TauI GCSGC 1 cut(s) 512
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 3 cut(s) 207, 296, 503
Tru9I TTAA 3 cut(s) 207, 296, 503
TscAI CASTG 2 cut(s) 12, 196
TseFI GTSAC 1 cut(s) 368
TseI GCWGC 3 cut(s) 17, 179, 344
Tsp45I GTSAC 1 cut(s) 368
TspDTI ATGAA 2 cut(s) 42, 517
TspRI CASTG 2 cut(s) 12, 196
VpaK11BI GGWCC 1 cut(s) 144
XapI RAATTY 1 cut(s) 357
XcmI CCANNNNNNNNNTGG 1 cut(s) 551
XmnI GAANNNNTTC 1 cut(s) 124
ZraI GACGTC 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.