Rh5BG134800

Ribose-5-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0006749
Physical Location & Seq
Forward (+)
13373815 .. 13378323
4509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG134800.1

Sequence Viewer

Length: 672 bp
ATGGCACTGGCAATGGCAGCCATACCAATAGCTTCACCAAATCCCCTGCATTCATCTCTAACTTTATCAAATGCCAGAGGCAGAAGAGCCTTCTGCACAAGAACAAGAACCCATTCTCTGAAGATTACTGGGTCCAGTCTTGCTGATGGCTCTGCCCTTTTTCAAGCAGCCCATTACACTGTGGATACCTACATTAAAAGTGGTATGGTGATTGGGTTGGGGTCTGGTATAGCTTCTGGTATGGCCATGCAGTATATAGGTCTGCAACTTGGAGCAGGTGCTTTAAAGGATATAGTGGGGATACCGACGTCTGTAGCATCTGCAAGTGAGGCAGCAAAGGCAGGAATTCCATTGGGTCACTATGAGGATAGTTCTCAAATTGATTTTGCATTTGATGATGCTGATATTATAGAAGAAAGGACTCTTATTGCTGTCATTGGGAGAACAAGATCGCAGGGTGAGGAGTCAATCATCCAAGAGAAGACTATATTAAACGCAGCCAATAAAGTTGTCTTCATAGTCAGAGAAAACCAGTATAAATGTGGTATGGATGGTTCTATTCCAGTTTTGGTGAATCCTCTCAACTGGATGGAAACTGCTGAAGAGATTGATGACCTGTTTTTAGGTGATGCAGAGGTCTGCTTCTATACTATGAAGTCAATATTTTGCTGA

Protein Analysis

223

Amino Acids

23.78

Weight (kDa)

5.06

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rib_5-P_isom_A PF06026 99 - 206 4.6e-20 Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 266
AatII GACGTC 1 cut(s) 311
Acc36I ACCTGC 1 cut(s) 266
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 345
AcuI CTGAAG 2 cut(s) 140, 621
AcyI GRCGYC 1 cut(s) 308
AgsI TTSAA 1 cut(s) 164
AluBI AGCT 2 cut(s) 32, 233
AluI AGCT 2 cut(s) 32, 233
AoxI GGCC 1 cut(s) 243
ApeKI GCWGC 4 cut(s) 17, 167, 332, 497
ApoI RAATTY 1 cut(s) 345
Asp700I GAANNNNTTC 1 cut(s) 112
AspS9I GGNCC 1 cut(s) 132
AsuHPI GGTGA 5 cut(s) 27, 220, 470, 583, 638
AvaII GGWCC 1 cut(s) 132
BaeI ACNNNNGTAYC 2 cut(s) 293, 326
BalI TGGCCA 1 cut(s) 245
BbsI GAAGAC 2 cut(s) 488, 505
BbvI GCAGC 4 cut(s) 29, 179, 344, 509
BccI CCATC 3 cut(s) 140, 545, 583
BciVI GTATCC 2 cut(s) 178, 294
BfmI CTRYAG 1 cut(s) 312
BfuAI ACCTGC 1 cut(s) 266
BfuI GTATCC 2 cut(s) 178, 294
BisI GCNGC 4 cut(s) 18, 168, 333, 498
BlsI GCNGC 4 cut(s) 19, 169, 334, 499
Bme18I GGWCC 1 cut(s) 132
BmgT120I GGNCC 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 133
BmrI ACTGGG 1 cut(s) 138
BmsI GCATC 3 cut(s) 326, 388, 619
BmuI ACTGGG 1 cut(s) 138
BpiI GAAGAC 2 cut(s) 488, 505
BsaHI GRCGYC 1 cut(s) 308
Bse1I ACTGG 6 cut(s) 12, 133, 135, 532, 563, 590
Bse3DI GCAATG 1 cut(s) 18
BseGI GGATG 3 cut(s) 471, 556, 594
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 6 cut(s) 12, 133, 135, 532, 563, 590
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 4 cut(s) 29, 179, 344, 509
BsgI GTGCAG 1 cut(s) 79
BshFI GGCC 1 cut(s) 245
BsmI GAATGC 1 cut(s) 49
BsnI GGCC 1 cut(s) 245
Bsp143I GATC 1 cut(s) 449
BspANI GGCC 1 cut(s) 245
BspLI GGNNCC 1 cut(s) 133
BspMI ACCTGC 1 cut(s) 266
BspQI GCTCTTC 1 cut(s) 79
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 6 cut(s) 12, 133, 135, 532, 563, 590
BssMI GATC 1 cut(s) 449
BssNI GRCGYC 1 cut(s) 308
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 2 cut(s) 79, 597
BstACI GRCGYC 1 cut(s) 308
BstF5I GGATG 3 cut(s) 471, 556, 594
BstKTI GATC 1 cut(s) 452
BstMBI GATC 1 cut(s) 449
BstMWI GCNNNNNNNGC 3 cut(s) 17, 329, 338
BstSFI CTRYAG 1 cut(s) 312
BstV1I GCAGC 4 cut(s) 29, 179, 344, 509
BstV2I GAAGAC 2 cut(s) 488, 505
BsuI GTATCC 2 cut(s) 178, 294
BsuRI GGCC 1 cut(s) 245
BtsCI GGATG 3 cut(s) 471, 556, 594
BtsIMutI CAGTG 2 cut(s) 5, 177
BveI ACCTGC 1 cut(s) 266
Cfr13I GGNCC 1 cut(s) 132
CviAII CATG 1 cut(s) 247
CviJI RGCY 8 cut(s) 20, 32, 89, 150, 170, 233, 245, 500
CviKI_1 RGCY 8 cut(s) 20, 32, 89, 150, 170, 233, 245, 500
DpnI GATC 1 cut(s) 451
DpnII GATC 1 cut(s) 449
DraI TTTAAA 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 2 cut(s) 79, 597
EarI CTCTTC 2 cut(s) 79, 597
Eco47I GGWCC 1 cut(s) 132
Eco57I CTGAAG 2 cut(s) 140, 621
EcoRI GAATTC 1 cut(s) 345
FaeI CATG 1 cut(s) 250
FatI CATG 1 cut(s) 246
Fnu4HI GCNGC 4 cut(s) 18, 168, 333, 498
FokI GGATG 3 cut(s) 458, 563, 601
Fsp4HI GCNGC 4 cut(s) 18, 168, 333, 498
GluI GCNGC 4 cut(s) 18, 168, 333, 498
HaeIII GGCC 1 cut(s) 245
Hin1I GRCGYC 1 cut(s) 308
Hin1II CATG 1 cut(s) 250
HinfI GANTC 3 cut(s) 421, 464, 574
HphI GGTGA 5 cut(s) 27, 220, 470, 583, 638
Hpy188I TCNGA 2 cut(s) 120, 524
Hpy99I CGWCG 1 cut(s) 310
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 308
HpyCH4V TGCA 7 cut(s) 49, 96, 250, 265, 323, 389, 632
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 329, 338
HpySE526I ACGT 1 cut(s) 308
Hsp92I GRCGYC 1 cut(s) 308
Hsp92II CATG 1 cut(s) 250
Kzo9I GATC 1 cut(s) 449
LguI GCTCTTC 1 cut(s) 79
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 4 cut(s) 29, 179, 344, 509
LweI GCATC 3 cut(s) 326, 388, 619
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 1 cut(s) 356
MalI GATC 1 cut(s) 451
MboI GATC 1 cut(s) 449
MboII GAAGA 6 cut(s) 96, 133, 425, 493, 505, 614
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 2 cut(s) 345, 378
MluNI TGGCCA 1 cut(s) 245
MlyI GAGTC 2 cut(s) 415, 473
MnlI CCTC 6 cut(s) 71, 322, 358, 454, 588, 628
Mox20I TGGCCA 1 cut(s) 245
MroXI GAANNNNTTC 1 cut(s) 112
MscI TGGCCA 1 cut(s) 245
MseI TTAA 3 cut(s) 195, 284, 491
Msp20I TGGCCA 1 cut(s) 245
Mva1269I GAATGC 1 cut(s) 49
MwoI GCNNNNNNNGC 3 cut(s) 17, 329, 338
NdeII GATC 1 cut(s) 449
NlaIII CATG 1 cut(s) 250
NlaIV GGNNCC 1 cut(s) 133
NmuCI GTSAC 1 cut(s) 356
PaqCI CACCTGC 1 cut(s) 266
PciSI GCTCTTC 1 cut(s) 79
PctI GAATGC 1 cut(s) 49
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 574
PkrI GCNGC 4 cut(s) 19, 169, 334, 499
PleI GAGTC 2 cut(s) 415, 472
PpsI GAGTC 2 cut(s) 415, 472
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 1 cut(s) 132
PsrI GAACNNNNNNTAC 2 cut(s) 538, 570
SapI GCTCTTC 1 cut(s) 79
SaqAI TTAA 3 cut(s) 195, 284, 491
SatI GCNGC 4 cut(s) 18, 168, 333, 498
Sau3AI GATC 1 cut(s) 449
Sau96I GGNCC 1 cut(s) 132
SchI GAGTC 2 cut(s) 415, 473
SetI ASST 9 cut(s) 34, 191, 235, 262, 280, 311, 618, 628, 639
SfaNI GCATC 3 cut(s) 326, 388, 619
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 1 cut(s) 132
Sse9I AATT 2 cut(s) 345, 378
SspI AATATT 1 cut(s) 663
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 1 cut(s) 311
TasI AATT 2 cut(s) 345, 378
TfiI GAWTC 1 cut(s) 574
Tru1I TTAA 3 cut(s) 195, 284, 491
Tru9I TTAA 3 cut(s) 195, 284, 491
TscAI CASTG 2 cut(s) 12, 184
TseFI GTSAC 1 cut(s) 356
TseI GCWGC 4 cut(s) 17, 167, 332, 497
Tsp45I GTSAC 1 cut(s) 356
TspDTI ATGAA 3 cut(s) 42, 505, 668
TspRI CASTG 2 cut(s) 12, 184
VpaK11BI GGWCC 1 cut(s) 132
XapI RAATTY 1 cut(s) 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 539
XmnI GAANNNNTTC 1 cut(s) 112
ZraI GACGTC 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.