FvH4_3g11560

Ribose-5-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0006749
Physical Location & Seq
Forward (+)
6822238 .. 6826579
4342 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g11560.t3

Sequence Viewer

Length: 900 bp
ATGTCACTGGCAATGGCAGCCATAGCAATAGCTTCACCAAATCCCCTGCATTCATCTCTAACTTTATCAAATGCCAGAGGAAGAAGATCCTTCTGCACAAGAAGAAGAACCCATTCTCTGAAGATTAGTGGGTCCAGTCTTGCTGATGGCTCTGCTCTTTTTCAAGCAGCCCATTACACTGTGGATACCTACATTAAGAGCGGTATGGTGATTGGGCTGGGATCTGGTATAGCTTCTGGTATGGCCATGCAGTATATAGGACTGCAACTTGGAGCTGGTGCTTTAAAGGATATAGTGGGGATACCAACGTCGGTAGCATCTGCAAGTGAGGCAGCGAAGGCAGGAATTCCATTGGGTCACTATGAGGATAGTTCTCAAATTGATTTTGCATTTGATGATGCTGATATTATAGAAGAAAGGACACTTATTGCTGTCATTGGGAGAACAAGATCGCAGGGTGAGGAGTCCATAATCCAAGAGAAGACTATACTAAATGCAGCAAATAAACTTGTCTTCATAGTCAGAGAAAACCAGTATAAATCTGGTATGGATGGTTCTATACCAGTTTTGGTGAACCCTGTCAACTGGATGGAAACTGCTGAAGAGATTGATGACTTGTTTTTAGGTGATGCAGAGCTCTGGAGGAGACCCTCAGTTGGGCTCGCAGGGCCTCATGGTGGTCAGTTCCCTCTAGTCACCAGAGAAGGCCATAACATTATTGATGTCATATTTACATCTCCAATTCTAAGCCTTGCCGAAGTATCGATGAGCCTTGATAACATTGATGGAGTCATAGAGCATGGAATCATTTCCAAGTTCCCATGCACTGCAGTAATCGCTTCGGAAAATGGGCTGTCTGTTGTTGATAATGTGCCTAAGAATGCAGTAAAGGAACCTTAA

Protein Analysis

300

Amino Acids

31.72

Weight (kDa)

5.16

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rib_5-P_isom_A PF06026 31 - 216 8.9e-37 Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 201
AciI CCGC 1 cut(s) 201
AclWI GGATC 2 cut(s) 81, 229
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 345
AcuI CTGAAG 2 cut(s) 140, 621
AfiI CCNNNNNNNGG 3 cut(s) 656, 657, 677
AgsI TTSAA 1 cut(s) 164
AluBI AGCT 4 cut(s) 32, 233, 275, 637
AluI AGCT 4 cut(s) 32, 233, 275, 637
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 1 cut(s) 640
AlwI GGATC 2 cut(s) 81, 229
AoxI GGCC 3 cut(s) 243, 668, 706
ApeKI GCWGC 4 cut(s) 17, 167, 332, 497
ApoI RAATTY 1 cut(s) 345
Asp700I GAANNNNTTC 2 cut(s) 112, 808
AspS9I GGNCC 2 cut(s) 132, 668
AsuHPI GGTGA 6 cut(s) 27, 220, 470, 583, 638, 688
AvaII GGWCC 1 cut(s) 132
BaeI ACNNNNGTAYC 2 cut(s) 293, 326
BalI TGGCCA 1 cut(s) 245
BanII GRGCYC 2 cut(s) 639, 663
BbsI GAAGAC 2 cut(s) 488, 505
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 4 cut(s) 29, 179, 344, 509
BccI CCATC 4 cut(s) 140, 545, 583, 779
BciVI GTATCC 2 cut(s) 178, 294
BcoDI GTCTC 1 cut(s) 640
BfaI CTAG 1 cut(s) 692
BfmI CTRYAG 1 cut(s) 828
BfuI GTATCC 2 cut(s) 178, 294
BisI GCNGC 4 cut(s) 18, 168, 333, 498
BlsI GCNGC 4 cut(s) 19, 169, 334, 499
Bme18I GGWCC 1 cut(s) 132
BmgT120I GGNCC 2 cut(s) 132, 668
BmiI GGNNCC 2 cut(s) 133, 894
BmsI GCATC 3 cut(s) 326, 388, 619
BpiI GAAGAC 2 cut(s) 488, 505
BpmI CTGGAG 1 cut(s) 661
Bsa29I ATCGAT 1 cut(s) 764
BsaI GGTCTC 1 cut(s) 640
Bsc4I CCNNNNNNNGG 3 cut(s) 656, 657, 677
Bse1I ACTGG 5 cut(s) 12, 135, 532, 563, 590
Bse3DI GCAATG 1 cut(s) 18
BseCI ATCGAT 1 cut(s) 764
BseGI GGATG 2 cut(s) 556, 594
BseLI CCNNNNNNNGG 3 cut(s) 656, 657, 677
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 5 cut(s) 12, 135, 532, 563, 590
BseRI GAGGAG 2 cut(s) 476, 658
BseXI GCAGC 4 cut(s) 29, 179, 344, 509
BseYI CCCAGC 1 cut(s) 217
BsgI GTGCAG 1 cut(s) 79
BshFI GGCC 3 cut(s) 245, 670, 708
BshVI ATCGAT 1 cut(s) 764
BsiHKAI GWGCWC 1 cut(s) 639
BslI CCNNNNNNNGG 3 cut(s) 656, 657, 677
BsmAI GTCTC 1 cut(s) 640
BsmI GAATGC 2 cut(s) 49, 886
BsnI GGCC 3 cut(s) 245, 670, 708
Bso31I GGTCTC 1 cut(s) 640
Bsp1286I GDGCHC 2 cut(s) 639, 663
Bsp143I GATC 3 cut(s) 86, 221, 449
BspACI CCGC 1 cut(s) 201
BspANI GGCC 3 cut(s) 245, 670, 708
BspCNI CTCAG 1 cut(s) 665
BspDI ATCGAT 1 cut(s) 764
BspLI GGNNCC 2 cut(s) 133, 894
BspMAI CTGCAG 1 cut(s) 832
BspPI GGATC 2 cut(s) 81, 229
BspTNI GGTCTC 1 cut(s) 640
BsrBI CCGCTC 1 cut(s) 201
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 5 cut(s) 12, 135, 532, 563, 590
BssMI GATC 3 cut(s) 86, 221, 449
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 1 cut(s) 597
BstC8I GCNNGC 1 cut(s) 663
BstDEI CTNAG 3 cut(s) 652, 746, 876
BstF5I GGATG 2 cut(s) 556, 594
BstKTI GATC 3 cut(s) 89, 224, 452
BstMAI GTCTC 1 cut(s) 640
BstMBI GATC 3 cut(s) 86, 221, 449
BstMWI GCNNNNNNNGC 6 cut(s) 17, 23, 329, 338, 667, 836
BstSFI CTRYAG 1 cut(s) 828
BstV1I GCAGC 4 cut(s) 29, 179, 344, 509
BstV2I GAAGAC 2 cut(s) 488, 505
BstX2I RGATCY 2 cut(s) 86, 221
BstYI RGATCY 2 cut(s) 86, 221
Bsu15I ATCGAT 1 cut(s) 764
BsuI GTATCC 2 cut(s) 178, 294
BsuRI GGCC 3 cut(s) 245, 670, 708
BsuTUI ATCGAT 1 cut(s) 764
BtsCI GGATG 2 cut(s) 556, 594
BtsI GCAGTG 1 cut(s) 825
BtsIMutI CAGTG 3 cut(s) 5, 177, 825
Cac8I GCNNGC 1 cut(s) 663
Cfr13I GGNCC 2 cut(s) 132, 668
ClaI ATCGAT 1 cut(s) 764
CviAII CATG 4 cut(s) 247, 674, 800, 822
DdeI CTNAG 3 cut(s) 652, 746, 876
DpnI GATC 3 cut(s) 88, 223, 451
DpnII GATC 3 cut(s) 86, 221, 449
DraI TTTAAA 1 cut(s) 285
EaeI YGGCCR 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
Ecl136II GAGCTC 1 cut(s) 637
Eco24I GRGCYC 2 cut(s) 639, 663
Eco31I GGTCTC 1 cut(s) 640
Eco47I GGWCC 1 cut(s) 132
Eco53kI GAGCTC 1 cut(s) 637
Eco57I CTGAAG 2 cut(s) 140, 621
EcoICRI GAGCTC 1 cut(s) 637
EcoO109I RGGNCCY 1 cut(s) 668
EcoRI GAATTC 1 cut(s) 345
EcoT38I GRGCYC 2 cut(s) 639, 663
FaeI CATG 4 cut(s) 250, 677, 803, 825
FatI CATG 4 cut(s) 246, 673, 799, 821
Fnu4HI GCNGC 4 cut(s) 18, 168, 333, 498
FokI GGATG 2 cut(s) 563, 601
FriOI GRGCYC 2 cut(s) 639, 663
Fsp4HI GCNGC 4 cut(s) 18, 168, 333, 498
FspBI CTAG 1 cut(s) 692
GluI GCNGC 4 cut(s) 18, 168, 333, 498
GsaI CCCAGC 1 cut(s) 221
GsuI CTGGAG 1 cut(s) 661
HaeIII GGCC 3 cut(s) 245, 670, 708
Hin1II CATG 4 cut(s) 250, 677, 803, 825
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HinfI GANTC 3 cut(s) 464, 789, 804
HphI GGTGA 6 cut(s) 27, 220, 470, 583, 638, 688
Hpy166II GTNNAC 2 cut(s) 574, 583
Hpy188I TCNGA 3 cut(s) 120, 524, 844
Hpy188III TCNNGA 1 cut(s) 640
Hpy8I GTNNAC 2 cut(s) 574, 583
Hpy99I CGWCG 1 cut(s) 313
HpyAV CCTTC 3 cut(s) 100, 331, 698
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 1 cut(s) 308
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 23, 329, 338, 667, 836
HpyF3I CTNAG 3 cut(s) 652, 746, 876
HpySE526I ACGT 1 cut(s) 308
Hsp92II CATG 4 cut(s) 250, 677, 803, 825
Kzo9I GATC 3 cut(s) 86, 221, 449
LmnI GCTCC 1 cut(s) 272
Lsp1109I GCAGC 4 cut(s) 29, 179, 344, 509
LweI GCATC 3 cut(s) 326, 388, 619
MaeI CTAG 1 cut(s) 692
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 3 cut(s) 3, 356, 694
MalI GATC 3 cut(s) 88, 223, 451
MbiI CCGCTC 1 cut(s) 201
MboI GATC 3 cut(s) 86, 221, 449
MboII GAAGA 9 cut(s) 93, 96, 114, 117, 133, 425, 493, 505, 614
MflI RGATCY 2 cut(s) 86, 221
MhlI GDGCHC 2 cut(s) 639, 663
MlsI TGGCCA 1 cut(s) 245
MluCI AATT 3 cut(s) 345, 378, 741
MluNI TGGCCA 1 cut(s) 245
MlyI GAGTC 2 cut(s) 473, 798
MnlI CCTC 8 cut(s) 71, 322, 358, 454, 636, 661, 681, 699
Mox20I TGGCCA 1 cut(s) 245
MroXI GAANNNNTTC 2 cut(s) 112, 808
MscI TGGCCA 1 cut(s) 245
MseI TTAA 3 cut(s) 195, 284, 898
Msp20I TGGCCA 1 cut(s) 245
Mva1269I GAATGC 2 cut(s) 49, 886
MwoI GCNNNNNNNGC 6 cut(s) 17, 23, 329, 338, 667, 836
NdeII GATC 3 cut(s) 86, 221, 449
NlaIII CATG 4 cut(s) 250, 677, 803, 825
NlaIV GGNNCC 2 cut(s) 133, 894
NmuCI GTSAC 3 cut(s) 3, 356, 694
PctI GAATGC 2 cut(s) 49, 886
PdmI GAANNNNTTC 2 cut(s) 112, 808
PfeI GAWTC 1 cut(s) 804
PkrI GCNGC 4 cut(s) 19, 169, 334, 499
PleI GAGTC 2 cut(s) 472, 797
PpsI GAGTC 2 cut(s) 472, 797
Psp124BI GAGCTC 1 cut(s) 639
PspFI CCCAGC 1 cut(s) 217
PspN4I GGNNCC 2 cut(s) 133, 894
PspPI GGNCC 2 cut(s) 132, 668
PsrI GAACNNNNNNTAC 2 cut(s) 538, 570
PstI CTGCAG 1 cut(s) 832
PsuI RGATCY 2 cut(s) 86, 221
SacI GAGCTC 1 cut(s) 639
SaqAI TTAA 3 cut(s) 195, 284, 898
SatI GCNGC 4 cut(s) 18, 168, 333, 498
Sau3AI GATC 3 cut(s) 86, 221, 449
Sau96I GGNCC 2 cut(s) 132, 668
SchI GAGTC 2 cut(s) 473, 798
SduI GDGCHC 2 cut(s) 639, 663
SetI ASST 8 cut(s) 34, 191, 235, 277, 311, 628, 639, 898
SfaNI GCATC 3 cut(s) 326, 388, 619
SfcI CTRYAG 1 cut(s) 828
SinI GGWCC 1 cut(s) 132
Sse9I AATT 3 cut(s) 345, 378, 741
SsiI CCGC 1 cut(s) 201
SspMI CTAG 1 cut(s) 692
SstI GAGCTC 1 cut(s) 639
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 1 cut(s) 311
TaqI TCGA 1 cut(s) 764
TasI AATT 3 cut(s) 345, 378, 741
TfiI GAWTC 1 cut(s) 804
Tru1I TTAA 3 cut(s) 195, 284, 898
Tru9I TTAA 3 cut(s) 195, 284, 898
TscAI CASTG 3 cut(s) 12, 184, 832
TseFI GTSAC 3 cut(s) 3, 356, 694
TseI GCWGC 4 cut(s) 17, 167, 332, 497
Tsp45I GTSAC 3 cut(s) 3, 356, 694
TspDTI ATGAA 2 cut(s) 42, 505
TspRI CASTG 3 cut(s) 12, 184, 832
VpaK11BI GGWCC 1 cut(s) 132
XapI RAATTY 1 cut(s) 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 539
XmnI GAANNNNTTC 2 cut(s) 112, 808
XspI CTAG 1 cut(s) 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.