Rorug05G0044800

Ribose-5-phosphate isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006749
Physical Location & Seq
Reverse (-)
3933021 .. 3937292
4272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0044800.1

Sequence Viewer

Length: 1557 bp
ATGCCACGGGCTTCTCTTAGTGCACTTGTGTCGGGGCTTATTCAGAATGGTGAACTGGATGAAGCTGCCAGGATTGTTGGTGGTGGATGTGGGAAGAGGGATGAGGGTGTGGAAGATTTGGTGGATGCTTATAATGCGTTGGTTGCTGGTTATGGTCAGAGAGGAAGGGTTGGAGAGGCTATGCGCCTTTTCGATCAAATACCATTTCGCCGTGACAAGGGGAATGAGGGAAGTAGGAGCTTCGCTTTGCTTTCTGTCAAGCCCACACATCTCTCTCCCTCCAAAGCCCAGATCACTTCCAAGCAGCTAACCCTTCTTTTGCTGAGTTCAAAGTCTTTGTGCCTGCTCAGTCTAGAATGTTCCATTGATCAGAGCATAGTGAATCTCTGTGATCTGTATGTGGTCTTGAAGATGTCTATCACCAAGCCATTAAAGAAATTAGTGTCGTGTGTTATCCTTGATTTGGATGGTACACTACTACACACAGATGGCATAGTAAGTGATGTTTTAAGAGTTTATTTGGGCAAGTATGGAAAGAAATGGGATGGGAGGGAAGTTCAAAAGATAGTTGGAAAAACACCACTCGAAGCTGCAACTGCTGTTGTGGAGGATTATGGGCTATCTTGCACAACGAGTGAATTACTCTCAGAGCTTGACCCAATGTTCTCAAATCAGTGGTGCAACATCAAAGCGCTTCCAGGTGCCAATCGGCTAATTAAACATTTGAGCGGTCACAGAGTGCCAATAGCATTGGCTTCAAACTCTCCAAGGGAAAACATAGAAACCAAAATTTCTTTTCATCAGGGTTGGAAAGAATCCTTCTTAGTTATCATTGGAGGTGACGAAGTAAGATCAGGAAAGCCATCTCCTGAAATATTCCTTGAAGCTGCTAAAAGATTAAATATTGAACCGTCCAGCTGTCTTGTCATTGAAGATTCTCTGCCAGGTGTGACAGCCGGGAAGGCTGCTGGAATGGAGGTAGTTGCTGTACCCTCTCTGCCAAAGCAATCCCATCTCTTTACTTCAGCAGATGAGGTGATCAACTCTCTACTTGATTTGCATCCTGAAAAGTGGGGCCTACCTCCTTTCAAAGATTGGATAAAAGACACTTTGCCAATAGAATCTTGGCAAATTGGTGGTCCTGTCATTAAGGGATTTGGTCGGGGCTCAAAAGTACTTGGGATCCCTACAGCTAATCTATCCACCGAGCGATATTCAACTCTCCTTTCAGAACATCCCTCGGGAGTATATTTCGGTTGGGCTGGATTACCAATGCGAGGTGTCTTTAAAATGGTCATGAGTATTGGTTGGAATCCTTATTTCAACAACACTGAAAAGACTATAGAGCCGTGGCTGCTTCATGACTTTGATGAGGACTTCTATGGGGAGGAATTGCATCTTATCATAGTTGGCTACATAAGGCCTGAGGCCAATTTCCCGTCCCTCGAGAGCTTGATCGAAAAGATCCATGAGGACCGGAAAATTGCAGAGGAAGCTCTTGATCTTCCAATGTACTCAAAATTCAAGGATGACCCATATCTTAAAGGCTCTACATAA

Protein Analysis

518

Amino Acids

57.0

Weight (kDa)

5.77

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 149 - 324 6.2e-24 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 151 - 330 2.3e-23 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 286 - 332 1.8e-06 HAD-hyrolase-like
Flavokinase PF01687 375 - 499 3.6e-35 Riboflavin kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 132
AccB1I GGYRCC 1 cut(s) 701
AccBSI CCGCTC 1 cut(s) 729
AciI CCGC 1 cut(s) 729
AclWI GGATC 3 cut(s) 1177, 1190, 1459
AcsI RAATTY 2 cut(s) 789, 1520
AcuI CTGAAG 1 cut(s) 1008
AdeI CACNNNGTG 1 cut(s) 739
AfaI GTAC 4 cut(s) 472, 990, 1176, 1514
AfeI AGCGCT 1 cut(s) 693
AfiI CCNNNNNNNGG 3 cut(s) 217, 463, 1277
AjnI CCWGG 3 cut(s) 68, 697, 943
Alw21I GWGCWC 1 cut(s) 25
Alw44I GTGCAC 1 cut(s) 21
AlwI GGATC 3 cut(s) 1177, 1190, 1459
Ama87I CYCGRG 2 cut(s) 1240, 1445
Aor51HI AGCGCT 1 cut(s) 693
AoxI GGCC 3 cut(s) 1075, 1421, 1428
ApaLI GTGCAC 1 cut(s) 21
ApeKI GCWGC 6 cut(s) 65, 304, 590, 887, 965, 1354
ApoI RAATTY 2 cut(s) 789, 1520
ArsI GACNNNNNNTTYG 2 cut(s) 1123, 1155
AspLEI GCGC 2 cut(s) 186, 694
AspS9I GGNCC 3 cut(s) 1075, 1139, 1474
AsuC2I CCSGG 1 cut(s) 958
AsuHPI GGTGA 4 cut(s) 62, 412, 851, 1048
AvaI CYCGRG 2 cut(s) 1240, 1445
AvaII GGWCC 2 cut(s) 1139, 1474
AxyI CCTNAGG 1 cut(s) 1425
BaeGI GKGCMC 1 cut(s) 25
BamHI GGATCC 1 cut(s) 1182
BanI GGYRCC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 1169
Bbv12I GWGCWC 1 cut(s) 25
BbvI GCAGC 6 cut(s) 52, 316, 577, 874, 952, 1341
BccI CCATC 5 cut(s) 461, 482, 539, 871, 1020
BceAI ACGGC 2 cut(s) 195, 1333
BcgI CGANNNNNNTGC 2 cut(s) 12, 46
BciT130I CCWGG 3 cut(s) 70, 699, 945
BclI TGATCA 2 cut(s) 367, 1038
BcnI CCSGG 1 cut(s) 958
BfaI CTAG 1 cut(s) 353
BfmI CTRYAG 2 cut(s) 1188, 1341
BfoI RGCGCY 1 cut(s) 695
BglI GCCNNNNNGGC 1 cut(s) 962
BisI GCNGC 6 cut(s) 66, 305, 591, 888, 966, 1355
BlsI GCNGC 6 cut(s) 67, 306, 592, 889, 967, 1356
BmcAI AGTACT 1 cut(s) 1176
Bme1390I CCNGG 4 cut(s) 70, 699, 945, 958
Bme18I GGWCC 2 cut(s) 1139, 1474
BmeT110I CYCGRG 2 cut(s) 1240, 1445
BmgT120I GGNCC 3 cut(s) 1075, 1139, 1474
BmiI GGNNCC 3 cut(s) 703, 1076, 1184
BmrFI CCNGG 4 cut(s) 70, 699, 945, 958
BmsI GCATC 3 cut(s) 115, 1069, 1405
BpuMI CCSGG 1 cut(s) 958
BsaBI GATNNNNATC 2 cut(s) 416, 1059
BsaJI CCNNGG 4 cut(s) 5, 767, 1239, 1349
BsaWI WCCGGW 1 cut(s) 1476
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 463, 1277
Bse1I ACTGG 1 cut(s) 60
Bse21I CCTNAGG 1 cut(s) 1425
Bse8I GATNNNNATC 2 cut(s) 416, 1059
BseBI CCWGG 3 cut(s) 70, 699, 945
BseDI CCNNGG 4 cut(s) 5, 767, 1239, 1349
BseGI GGATG 9 cut(s) 64, 92, 106, 130, 472, 550, 1060, 1234, 1534
BseJI GATNNNNATC 2 cut(s) 416, 1059
BseLI CCNNNNNNNGG 3 cut(s) 217, 463, 1277
BseMII CTCAG 4 cut(s) 314, 361, 660, 1416
BseNI ACTGG 1 cut(s) 60
BseSI GKGCMC 1 cut(s) 25
BseXI GCAGC 6 cut(s) 52, 316, 577, 874, 952, 1341
BshFI GGCC 3 cut(s) 1077, 1423, 1430
BshNI GGYRCC 1 cut(s) 701
BsiHKAI GWGCWC 1 cut(s) 25
BsiHKCI CYCGRG 2 cut(s) 1240, 1445
BsiSI CCGG 2 cut(s) 957, 1477
BslFI GGGAC 1 cut(s) 1426
BslI CCNNNNNNNGG 3 cut(s) 217, 463, 1277
BsmFI GGGAC 1 cut(s) 1426
BsnI GGCC 3 cut(s) 1077, 1423, 1430
BsoBI CYCGRG 2 cut(s) 1240, 1445
Bsp1286I GDGCHC 2 cut(s) 25, 1169
BspACI CCGC 1 cut(s) 729
BspANI GGCC 3 cut(s) 1077, 1423, 1430
BspCNI CTCAG 4 cut(s) 315, 360, 659, 1417
BspHI TCATGA 2 cut(s) 1296, 1360
BspLI GGNNCC 3 cut(s) 703, 1076, 1184
BspPI GGATC 3 cut(s) 1177, 1190, 1459
BspT107I GGYRCC 1 cut(s) 701
BsrBI CCGCTC 1 cut(s) 729
BsrI ACTGG 1 cut(s) 60
BssECI CCNNGG 4 cut(s) 5, 767, 1239, 1349
BssT1I CCWWGG 1 cut(s) 767
Bst2UI CCWGG 3 cut(s) 70, 699, 945
Bst4CI ACNGT 1 cut(s) 912
Bst6I CTCTTC 1 cut(s) 89
BstC8I GCNNGC 1 cut(s) 344
BstDEI CTNAG 6 cut(s) 17, 323, 347, 646, 823, 1425
BstDSI CCRYGG 2 cut(s) 5, 1349
BstF5I GGATG 9 cut(s) 64, 92, 106, 130, 472, 550, 1060, 1234, 1534
BstH2I RGCGCY 1 cut(s) 695
BstHHI GCGC 2 cut(s) 186, 694
BstMWI GCNNNNNNNGC 6 cut(s) 134, 143, 596, 962, 1354, 1493
BstNI CCWGG 3 cut(s) 70, 699, 945
BstSCI CCNGG 4 cut(s) 68, 697, 943, 956
BstSFI CTRYAG 2 cut(s) 1188, 1341
BstSLI GKGCMC 1 cut(s) 25
BstV1I GCAGC 6 cut(s) 52, 316, 577, 874, 952, 1341
BstX2I RGATCY 2 cut(s) 1182, 1464
BstYI RGATCY 2 cut(s) 1182, 1464
Bsu36I CCTNAGG 1 cut(s) 1425
BsuRI GGCC 3 cut(s) 1077, 1423, 1430
BtgI CCRYGG 2 cut(s) 5, 1349
BtsCI GGATG 9 cut(s) 64, 92, 106, 130, 472, 550, 1060, 1234, 1534
BtsIMutI CAGTG 2 cut(s) 680, 1329
Cac8I GCNNGC 1 cut(s) 344
CciI TCATGA 2 cut(s) 1296, 1360
CfoI GCGC 2 cut(s) 186, 694
Cfr13I GGNCC 3 cut(s) 1075, 1139, 1474
Csp6I GTAC 4 cut(s) 471, 989, 1175, 1513
CviAII CATG 3 cut(s) 1297, 1361, 1469
CviQI GTAC 4 cut(s) 471, 989, 1175, 1513
DdeI CTNAG 6 cut(s) 17, 323, 347, 646, 823, 1425
DraI TTTAAA 1 cut(s) 1288
DraIII CACNNNGTG 1 cut(s) 739
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 767
Eco147I AGGCCT 1 cut(s) 1423
Eco24I GRGCYC 1 cut(s) 1169
Eco47I GGWCC 2 cut(s) 1139, 1474
Eco47III AGCGCT 1 cut(s) 693
Eco57I CTGAAG 1 cut(s) 1008
Eco81I CCTNAGG 1 cut(s) 1425
Eco88I CYCGRG 2 cut(s) 1240, 1445
EcoO109I RGGNCCY 1 cut(s) 1075
EcoRII CCWGG 3 cut(s) 68, 697, 943
EcoT14I CCWWGG 1 cut(s) 767
EcoT38I GRGCYC 1 cut(s) 1169
ErhI CCWWGG 1 cut(s) 767
FaeI CATG 3 cut(s) 1300, 1364, 1472
FaqI GGGAC 1 cut(s) 1426
FatI CATG 3 cut(s) 1296, 1360, 1468
FbaI TGATCA 2 cut(s) 367, 1038
Fnu4HI GCNGC 6 cut(s) 66, 305, 591, 888, 966, 1355
FokI GGATG 9 cut(s) 71, 99, 113, 137, 479, 557, 1047, 1221, 1541
FriOI GRGCYC 1 cut(s) 1169
Fsp4HI GCNGC 6 cut(s) 66, 305, 591, 888, 966, 1355
FspBI CTAG 1 cut(s) 353
GlaI GCGC 2 cut(s) 185, 693
GluI GCNGC 6 cut(s) 66, 305, 591, 888, 966, 1355
HaeII RGCGCY 1 cut(s) 695
HaeIII GGCC 3 cut(s) 1077, 1423, 1430
HapII CCGG 2 cut(s) 957, 1477
HhaI GCGC 2 cut(s) 186, 694
Hin1II CATG 3 cut(s) 1300, 1364, 1472
Hin6I GCGC 2 cut(s) 184, 692
HinP1I GCGC 2 cut(s) 184, 692
HinfI GANTC 5 cut(s) 382, 815, 935, 1121, 1312
HpaII CCGG 2 cut(s) 957, 1477
HphI GGTGA 4 cut(s) 62, 412, 851, 1048
Hpy166II GTNNAC 3 cut(s) 23, 53, 473
Hpy188I TCNGA 5 cut(s) 45, 159, 372, 649, 1231
Hpy8I GTNNAC 3 cut(s) 23, 53, 473
HpyAV CCTTC 4 cut(s) 159, 323, 829, 955
HpyCH4III ACNGT 1 cut(s) 912
HpyCH4V TGCA 7 cut(s) 23, 593, 627, 681, 1060, 1396, 1487
HpyF10VI GCNNNNNNNGC 6 cut(s) 134, 143, 596, 962, 1354, 1493
HpyF3I CTNAG 6 cut(s) 17, 323, 347, 646, 823, 1425
Hsp92II CATG 3 cut(s) 1300, 1364, 1472
HspAI GCGC 2 cut(s) 184, 692
Ksp22I TGATCA 2 cut(s) 367, 1038
LmnI GCTCC 1 cut(s) 237
Lsp1109I GCAGC 6 cut(s) 52, 316, 577, 874, 952, 1341
LweI GCATC 3 cut(s) 115, 1069, 1405
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 4 cut(s) 212, 731, 839, 949
MbiI CCGCTC 1 cut(s) 729
MboII GAAGA 5 cut(s) 106, 125, 421, 944, 1496
MflI RGATCY 2 cut(s) 1182, 1464
MhlI GDGCHC 2 cut(s) 25, 1169
MluCI AATT 9 cut(s) 437, 638, 714, 789, 1131, 1391, 1432, 1482, 1520
MmeI TCCRAC 4 cut(s) 151, 550, 788, 1289
MseI TTAA 7 cut(s) 431, 509, 717, 899, 1149, 1287, 1542
MslI CAYNNNNRTG 1 cut(s) 486
MspA1I CMGCKG 1 cut(s) 918
MspI CCGG 2 cut(s) 957, 1477
MspR9I CCNGG 4 cut(s) 70, 699, 945, 958
MvaI CCWGG 3 cut(s) 70, 699, 945
MwoI GCNNNNNNNGC 6 cut(s) 134, 143, 596, 962, 1354, 1493
NciI CCSGG 1 cut(s) 958
NlaIII CATG 3 cut(s) 1300, 1364, 1472
NlaIV GGNNCC 3 cut(s) 703, 1076, 1184
NmuCI GTSAC 4 cut(s) 212, 731, 839, 949
PaeR7I CTCGAG 1 cut(s) 1445
PagI TCATGA 2 cut(s) 1296, 1360
PceI AGGCCT 1 cut(s) 1423
PfeI GAWTC 5 cut(s) 382, 815, 935, 1121, 1312
PkrI GCNGC 6 cut(s) 67, 306, 592, 889, 967, 1356
PsiI TTATAA 1 cut(s) 132
Psp6I CCWGG 3 cut(s) 68, 697, 943
PspGI CCWGG 3 cut(s) 68, 697, 943
PspN4I GGNNCC 3 cut(s) 703, 1076, 1184
PspPI GGNCC 3 cut(s) 1075, 1139, 1474
PsuI RGATCY 2 cut(s) 1182, 1464
PvuII CAGCTG 1 cut(s) 918
RsaI GTAC 4 cut(s) 472, 990, 1176, 1514
RsaNI GTAC 4 cut(s) 471, 989, 1175, 1513
RseI CAYNNNNRTG 1 cut(s) 486
SaqAI TTAA 7 cut(s) 431, 509, 717, 899, 1149, 1287, 1542
SatI GCNGC 6 cut(s) 66, 305, 591, 888, 966, 1355
Sau96I GGNCC 3 cut(s) 1075, 1139, 1474
ScaI AGTACT 1 cut(s) 1176
ScrFI CCNGG 4 cut(s) 70, 699, 945, 958
SduI GDGCHC 2 cut(s) 25, 1169
SfaNI GCATC 3 cut(s) 115, 1069, 1405
SfcI CTRYAG 2 cut(s) 1188, 1341
Sfr274I CTCGAG 1 cut(s) 1445
SinI GGWCC 2 cut(s) 1139, 1474
SlaI CTCGAG 1 cut(s) 1445
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 1 cut(s) 1445
SmoI CTYRAG 1 cut(s) 1445
Sse9I AATT 9 cut(s) 437, 638, 714, 789, 1131, 1391, 1432, 1482, 1520
SseBI AGGCCT 1 cut(s) 1423
SsiI CCGC 1 cut(s) 729
SspI AATATT 2 cut(s) 876, 904
SspMI CTAG 1 cut(s) 353
StuI AGGCCT 1 cut(s) 1423
StyD4I CCNGG 4 cut(s) 68, 697, 943, 956
StyI CCWWGG 1 cut(s) 767
TaaI ACNGT 1 cut(s) 912
TaqI TCGA 4 cut(s) 192, 585, 1446, 1458
TasI AATT 9 cut(s) 437, 638, 714, 789, 1131, 1391, 1432, 1482, 1520
TatI WGTACW 2 cut(s) 1174, 1512
TfiI GAWTC 5 cut(s) 382, 815, 935, 1121, 1312
Tru1I TTAA 7 cut(s) 431, 509, 717, 899, 1149, 1287, 1542
Tru9I TTAA 7 cut(s) 431, 509, 717, 899, 1149, 1287, 1542
TscAI CASTG 2 cut(s) 680, 1336
TseFI GTSAC 4 cut(s) 212, 731, 839, 949
TseI GCWGC 6 cut(s) 65, 304, 590, 887, 965, 1354
Tsp45I GTSAC 4 cut(s) 212, 731, 839, 949
TspDTI ATGAA 3 cut(s) 75, 788, 1349
TspRI CASTG 2 cut(s) 680, 1336
VneI GTGCAC 1 cut(s) 21
VpaK11BI GGWCC 2 cut(s) 1139, 1474
XapI RAATTY 2 cut(s) 789, 1520
XbaI TCTAGA 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 1122
XhoI CTCGAG 1 cut(s) 1445
XspI CTAG 1 cut(s) 353
ZrmI AGTACT 1 cut(s) 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.