Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003584

Physical Location & Seq

Genomic Coordinates Reverse (-)

18247149 .. 18258197

Gene Structure 11049 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G45140.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3486 bp

ATGGGACTTGATCAAGAAGATTTAGACTTGACCAATGATGATCATTTCATTGACAAGGAAAAGCTTTCAGCTCCTATCAAGTCTACTGCAGATAAATTCCAGCTCGTTCCTGAGTTCTTGAAGGTGAGAGGACTTGTGAAGCAACACCTGGATTCTTTCAACTACTTTATCAATGTCGGGATTCATAAGATTGTTAAGGCAAATAGTCGTATTACATCCACAGTGGACCCATCAATCTACCTAAGGTTTAAGAAGGTCAGAGTTGGTGAACCGTCAATTATAAACGTTAATACGGTGGAAAATATTAATCCGCACATGTGCCGATTAGCAGACATGACATATGCTGCTCCAATATTTGTCAATATAGAATATGTTCACGGAAGTCATGGAAACAAGGCAAAAAGTGCAAAGGATAATGTAATTATCGGAAGGATGCCTATTATGTTGCGGAGTTGCCGCTGTGTATTACATGGAAAAGACGAAGAGGAACTTGCAAGATTGGGTGAGTGCCCTCTTGATCCTGGAGGATACTTCATCATTAAAGGAACTGAGAAGGTGTTGTTGATACAAGAACAACTTTCGAAGAACAGAATTATTATTGATTCTGATAAAAAGGGGAACATAAATGCATCCGTCACAAGCAGCACCGAAATGACAAAAAGCAAAACAGTTATTCAGATGGAAAAGGAGAAGATATATTTATTTCTTCATCGATTTGTAAAAAAGATTCCAATTATAATTGTCCTAAAAGCTATGGGAATGGAGAGCGACCAAGAGATTGTACAAATGGTTGGAAGGGATCCCCGTTTTAGCGCGTCACTCTTACCTTCTATTGAGGAGTGTGTTAGCGAAGGTGTGAATACACAAAAACAAGCACTGGACTATCTTGAGGCAAAGGTGAAAAAAATATCATACGGAACTCCACCTGAAAAGGACGGAAGAGCTCTATCCATCCTCAGAGATTTATTCCTTGCGCATGTACCAGTGCCTGACAACAATTTTCGTCAAAAATGTTTCTACGTTGGTGTGATGTTGAGACGAATGATTGAAGCAATGTTAAACAAAGATGCGATGGATGACAAGGATTATGTTGGTAACAAGCGGTTGGAGTTATCTGGACAACTAATATCTCTTCTCTTTGAGGATTTGTTCAAAACGATGCTCAGTGAGGCCATTAAAAATGTAGATCATATTCTTAACAAACCAATCCGGGCTAGCCGATTTGATTTTTCTCAGTGTCTTAATAAAGACAGTCGTTACAGTATTTCTCTCGGACTAGAAAGAACCCTTTCAACTGGAAACTTCGATATTAAAAGGTTCAGAATGCATAGAAAAGGCATGACACAGGTCTTAACAAGGTTATCTTTTATCGGGAGCATGGGGTTTATCACGAAAATTTCGCCACAATTTGAAAAGTCCAGAAAAGTAAGTGGGCCTAGATCATTGCAACCCAGCCAGTGGGGCATGCTATGCCCATGTGATACCCCAGAAGGCGAATCTTGTGGGCTTGTCAAGAACTTGGCTTTAATGACTCATGTCACAACAGATGAAGAGGAAGGCCCATTGGTTGCTATGTGCTACAAATTGGGTGTTACAGATCTGGAAGTATTGTCAGCAGAGGAGCTGCATACTCCGGACTCATTCTTGGTCATATTGAATGGGCTAATACTTGGCAAACATAGTAGGCCACAGTACTTCGCCAATTCACTTAGAAGGCTGCGTAGAGCTGGAAAAATTGGAGAGTTTGTCAGTGTGTTCACAAACGAAAAGCAGCATTGTGTTTACGTTGCTTCTGATGTTGGGCGGGTTTGCCGTCCACTTGTTATAGCCGATAAAGGAATATCAAGAGTTAAACAACACCACATGAAGGAATTACAGGATGGGGTTCGCACTTTTGATGACTTTATTCGTGACGGTTTGATAGAGTACCTTGATGTCAATGAGGAAAACAATGCATTGATCGCTTTATATGAAAGTGATGGCACTACGGAACTGGATGAAGGAGCAGAAGCAGCTAAGGCAGACACAACCCACATTGAGATTGAACCTTTCACCATCTTAGGTGTTGTTGCTGGTCTTATTCCCTACCCTCACCATAATCAGTCACCTAGAAATACATATCAGTGTGCTATGGGGAAACAAGCTATGGGAAATATTGCTTACAACCAGTTGAACCGGATGGACACATTGCTTTACCTATTGGTGTATCCTCAACGACCCCTGTTGACAACAAGGACAATTGAATTGGTTGGATACGATAAACTTGGAGCAGGTCAAAATGCAACAGTTGCTGTCATGAGTTTTAGTGGATACGACATAGAGGATGCAATAGTGATGAACAAATCTTCCTTGGATCGTGGTTTTGGCCGCTGCATTGTTATGAAAAAAATTGTTGCTATGTCCCAGAAGTATGACAACTGTACAGCAGACAGAATACTCATTCCACAAAGAACAGGCCCAGATGCAGAAAAGATGCAGATACTGGATGATGATGGATTAGCTACTCCAGGGGAGATTATCAGACCGAATGATATTTATATAAATAAACAAGTCCCTGTGGACACAGTAACTAAGTTTACATCTGCGTTATCTGATAGTCAATACCGTCCGGCCAGAGAGTATTTCAAAGGTCCTGAAGGGGAGACGCAAGTGGTCGATAGAGTTGCTCTCTGTTCTGATAAGAAAGGCCAGTTGTGCATCAAATATATCATACGCCATACTCGTCGACCAGAGCTTGGCGACAAATTCAGCAGTAGGCATGGGCAGAAAGGTGTATGTGGTATAATTATTCAGCAAGAAGATTTTCCATTTTCTGAGCTTGGAATTTGCCCTGATTTGATCATGAATCCTCATGGGTTTCCAAGTCGTATGACTGTAGGTAAGATGATAGAACTCCTTGGAAGTAAGGCTGGGGTTTCTTGTGGTAGATTTCATTATGGTAGTGCTTTTGGAGAAAGAAGTGGTCATGCTGACAAGGTTGAGACTATAAGTGCGACCCTTGTGGAAAAAGGGTTTTCATACAGTGGGAAGGACTTGTTATACTCAGGTATCAGTGGCGAGCCAGTGGAAGCGTACATCTTTATGGGGCCAATATATTACCAGAAATTGAAACACATGGTTCTGGACAAGATGCATGCGCGAGGGAGTGGACCACGTGTTATGATGACAAGACAGCCTACTGAAGGGAAATCTAAGAATGGAGGTTTAAGAGTGGGAGAAATGGAAAGAGATTGTCTAATTGCTTATGGAGCCAGCATGTTGATCTACGAGCGGCTCATGATATCTAGTGATCCTTTCGAAGTTCAGGTTTGCAGAGCTTGTGGTTTGTTGGGTTATTACAATTACAAGCTTAAGAAAGCAGTTTGTACAACATGTAAAAATGGCGATAACATTGCTACTATGAAACTCCCATACGCATGCAAGCTACTATTCCAGGAACTCCAGTCGATGAATGTTGTTCCGCGTCTGAAACTCACAGAGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006383 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032501 GO:0032502 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045089 GO:0046483 GO:0048229 GO:0048518 GO:0048583 GO:0048584 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080134 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1161

Amino Acids

130.21

Weight (kDa)

8.49

Isoelectric Point (pI)

39.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	44 - 403	6e-53	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	195 - 370	1.7e-27	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	449 - 513	2.3e-18	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	550 - 610	1.1e-21	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_5	PF04567	632 - 683	3.2e-10	RNA polymerase Rpb2, domain 5
RNA_pol_Rpb2_6	PF00562	690 - 813	8.4e-42	RNA polymerase Rpb2, domain 6

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45140 AT5G45140 AT5G45140
fragaria_vesca	FvH4_5g31720 FvH4_7g03930
malus_domestica	MD14G1029000.v1.1
prunus_persica	Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1
pyrus_communis	pycom14g02710
rosa_chinensis	RchiOBHm_Chr1g0327591 RchiOBHm_Chr7g0227611
rosa_laevigata	RLG00000001632 RLG00000029981
rosa_multiflora	Rmu_co8491793.1_g000001 Rmu_sc0001772.1_g000005 Rmu_ssc0000042.1_g000027
rosa_roxburghii	Rroxscaffold_3G00232000 Rroxscaffold_4G00322550
rosa_rugosa	Rorug01G0068500 Rorug07G0242400
rosa_samantha	Rh1AG085800 Rh1BG068400 Rh1CG083300 Rh1DG090000 Rh7AG395800 Rh7BG377500 Rh7CG414500 Rh7DG391600
rosa_wichuraiana	Rw1G006690 Rw7G032920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	281, 737
Acc16I	TGCGCA	1 cut(s)	975
Acc36I	ACCTGC	1 cut(s)	2261
AccB7I	CCANNNNNTGG	2 cut(s)	1458, 2735
AccBSI	CCGCTC	1 cut(s)	3271
AccI	GTMKAC	2 cut(s)	83, 2725
AccII	CGCG	3 cut(s)	815, 3139, 3463
AccIII	TCCGGA	1 cut(s)	1633
AciI	CCGC	8 cut(s)	311, 448, 457, 1102, 1804, 2368, 3271, 3461
AclI	AACGTT	1 cut(s)	285
AclWI	GGATC	5 cut(s)	512, 794, 807, 2361, 3284
AcoI	YGGCCR	2 cut(s)	2365, 2610
AcsI	RAATTY	4 cut(s)	95, 1395, 2744, 2823
AcuI	CTGAAG	2 cut(s)	2655, 3201
AcvI	CACGTG	1 cut(s)	3155
AfaI	GTAC	7 cut(s)	783, 981, 1694, 1928, 2422, 3074, 3367
AfiI	CCNNNNNNNGG	4 cut(s)	1458, 1867, 2735, 3182
AflII	CTTAAG	1 cut(s)	3350
AflIII	ACRYGT	3 cut(s)	315, 3154, 3371
AjnI	CCWGG	4 cut(s)	147, 520, 2506, 3432
AloI	GAACNNNNNNTCC	2 cut(s)	2330, 2362
Alw21I	GWGCWC	1 cut(s)	946
Alw26I	GTCTC	3 cut(s)	1030, 2636, 2975
AlwI	GGATC	5 cut(s)	512, 794, 807, 2361, 3284
AlwNI	CAGNNNCTG	3 cut(s)	989, 2291, 2482
Aor13HI	TCCGGA	1 cut(s)	1633
AoxI	GGCC	9 cut(s)	1170, 1433, 1558, 1685, 2365, 2455, 2610, 2686, 3086
ApeKI	GCWGC	7 cut(s)	344, 642, 1624, 1717, 1771, 2012, 2370
ApoI	RAATTY	4 cut(s)	95, 1395, 2744, 2823
AseI	ATTAAT	1 cut(s)	306
Asp700I	GAANNNNTTC	3 cut(s)	372, 1288, 2802
AspLEI	GCGC	3 cut(s)	815, 976, 3139
AspS9I	GGNCC	7 cut(s)	226, 1433, 1559, 2456, 2630, 3086, 3149
AsuC2I	CCSGG	1 cut(s)	1211
AsuHPI	GGTGA	7 cut(s)	136, 278, 515, 910, 2044, 2084, 2097
AsuII	TTCGAA	2 cut(s)	581, 3297
AsuNHI	GCTAGC	1 cut(s)	1214
AvaII	GGWCC	3 cut(s)	226, 2630, 3149
AxyI	CCTNAGG	1 cut(s)	242
BaeGI	GKGCMC	1 cut(s)	512
BaeI	ACNNNNGTAYC	2 cut(s)	3031, 3064
BamHI	GGATCC	1 cut(s)	799
BanII	GRGCYC	1 cut(s)	946
BbrPI	CACGTG	1 cut(s)	3155
Bbv12I	GWGCWC	1 cut(s)	946
BbvI	GCAGC	7 cut(s)	331, 654, 1611, 1704, 1783, 2024, 2357
BccI	CCATC	9 cut(s)	238, 673, 959, 1066, 1874, 1973, 2063, 2173, 2486
BceAI	ACGGC	1 cut(s)	1797
BcgI	CGANNNNNNTGC	4 cut(s)	2645, 2679, 3374, 3408
BciT130I	CCWGG	4 cut(s)	149, 522, 2508, 3434
BciVI	GTATCC	4 cut(s)	521, 2217, 2246, 2303
BclI	TGATCA	3 cut(s)	10, 40, 2838
BcnI	CCSGG	1 cut(s)	1211
BcoDI	GTCTC	3 cut(s)	1030, 2636, 2975
BfaI	CTAG	5 cut(s)	1215, 1277, 1437, 2109, 3285
BfmI	CTRYAG	2 cut(s)	87, 2874
BfrI	CTTAAG	1 cut(s)	3350
BfuAI	ACCTGC	1 cut(s)	2261
BfuI	GTATCC	4 cut(s)	521, 2217, 2246, 2303
BglI	GCCNNNNNGGC	1 cut(s)	1461
BglII	AGATCT	1 cut(s)	1597
BmcAI	AGTACT	1 cut(s)	1694
Bme1390I	CCNGG	5 cut(s)	149, 522, 1211, 2508, 3434
Bme18I	GGWCC	3 cut(s)	226, 2630, 3149
BmgT120I	GGNCC	7 cut(s)	226, 1433, 1559, 2456, 2630, 3086, 3149
BmiI	GGNNCC	4 cut(s)	228, 801, 3087, 3250
BmrFI	CCNGG	5 cut(s)	149, 522, 1211, 2508, 3434
BmsI	GCATC	9 cut(s)	423, 638, 1057, 1149, 2314, 2452, 2463, 2706, 3120
BmtI	GCTAGC	1 cut(s)	1218
BoxI	GACNNNNGTC	2 cut(s)	1346, 1535
BplI	GAGNNNNNCTC	2 cut(s)	2652, 2684
BpmI	CTGGAG	3 cut(s)	543, 2490, 3425
Bpu10I	CCTNAGC	1 cut(s)	2016
Bpu14I	TTCGAA	2 cut(s)	581, 3297
BpuEI	CTTGAG	1 cut(s)	908
BpuMI	CCSGG	1 cut(s)	1211
Bsa29I	ATCGAT	1 cut(s)	712
BsaAI	YACGTR	1 cut(s)	3155
BsaJI	CCNNGG	3 cut(s)	2349, 2507, 2896
BsaWI	WCCGGW	2 cut(s)	1633, 2175
BsaXI	ACNNNNNCTCC	2 cut(s)	1731, 1761
Bsc4I	CCNNNNNNNGG	4 cut(s)	1458, 1867, 2735, 3182
Bse21I	CCTNAGG	1 cut(s)	242
Bse3DI	GCAATG	4 cut(s)	1059, 1442, 2186, 3390
BseAI	TCCGGA	1 cut(s)	1633
BseBI	CCWGG	4 cut(s)	149, 522, 2508, 3434
BseCI	ATCGAT	1 cut(s)	712
BseDI	CCNNGG	3 cut(s)	2349, 2507, 2896
BseLI	CCNNNNNNNGG	4 cut(s)	1458, 1867, 2735, 3182
BseMI	GCAATG	4 cut(s)	1059, 1442, 2186, 3390
BseMII	CTCAG	7 cut(s)	102, 540, 970, 1177, 1247, 2805, 3057
BseRI	GAGGAG	2 cut(s)	851, 1634
BseSI	GKGCMC	1 cut(s)	512
BseXI	GCAGC	7 cut(s)	331, 654, 1611, 1704, 1783, 2024, 2357
BseYI	CCCAGC	2 cut(s)	1451, 2909
Bsh1236I	CGCG	3 cut(s)	815, 3139, 3463
BshFI	GGCC	9 cut(s)	1172, 1435, 1560, 1687, 2367, 2457, 2612, 2688, 3088
BshVI	ATCGAT	1 cut(s)	712
BsiHKAI	GWGCWC	1 cut(s)	946
BsiSI	CCGG	4 cut(s)	1210, 1634, 2176, 2609
BslFI	GGGAC	3 cut(s)	18, 2386, 2537
BslI	CCNNNNNNNGG	4 cut(s)	1458, 1867, 2735, 3182
BsmAI	GTCTC	3 cut(s)	1030, 2636, 2975
BsmBI	CGTCTC	2 cut(s)	1030, 2636
BsmFI	GGGAC	3 cut(s)	18, 2386, 2537
BsmI	GAATGC	1 cut(s)	1329
BsnI	GGCC	9 cut(s)	1172, 1435, 1560, 1687, 2367, 2457, 2612, 2688, 3088
Bsp119I	TTCGAA	2 cut(s)	581, 3297
Bsp1286I	GDGCHC	2 cut(s)	512, 946
Bsp13I	TCCGGA	1 cut(s)	1633
Bsp1407I	TGTACA	3 cut(s)	781, 2420, 3365
BspACI	CCGC	8 cut(s)	311, 448, 457, 1102, 1804, 2368, 3271, 3461
BspANI	GGCC	9 cut(s)	1172, 1435, 1560, 1687, 2367, 2457, 2612, 2688, 3088
BspCNI	CTCAG	7 cut(s)	103, 541, 969, 1176, 1246, 2806, 3056
BspDI	ATCGAT	1 cut(s)	712
BspEI	TCCGGA	1 cut(s)	1633
BspFNI	CGCG	3 cut(s)	815, 3139, 3463
BspHI	TCATGA	3 cut(s)	2295, 2841, 3276
BspLI	GGNNCC	4 cut(s)	228, 801, 3087, 3250
BspMAI	CTGCAG	1 cut(s)	91
BspMI	ACCTGC	1 cut(s)	2261
BspOI	GCTAGC	1 cut(s)	1218
BspPI	GGATC	5 cut(s)	512, 794, 807, 2361, 3284
BspQI	GCTCTTC	1 cut(s)	934
BspT104I	TTCGAA	2 cut(s)	581, 3297
BspTI	CTTAAG	1 cut(s)	3350
BsrBI	CCGCTC	1 cut(s)	3271
BsrDI	GCAATG	4 cut(s)	1059, 1442, 2186, 3390
BsrGI	TGTACA	3 cut(s)	781, 2420, 3365
BssECI	CCNNGG	3 cut(s)	2349, 2507, 2896
BssT1I	CCWWGG	2 cut(s)	2349, 2896
Bst2UI	CCWGG	4 cut(s)	149, 522, 2508, 3434
Bst6I	CTCTTC	4 cut(s)	477, 934, 1137, 1545
BstAFI	CTTAAG	1 cut(s)	3350
BstAPI	GCANNNNNTGC	3 cut(s)	404, 1470, 2288
BstAUI	TGTACA	3 cut(s)	781, 2420, 3365
BstBAI	YACGTR	1 cut(s)	3155
BstBI	TTCGAA	2 cut(s)	581, 3297
BstC8I	GCNNGC	7 cut(s)	1216, 1466, 3059, 3135, 3253, 3418, 3422
BstENI	CCTNNNNNAGG	1 cut(s)	3180
BstFNI	CGCG	3 cut(s)	815, 3139, 3463
BstHHI	GCGC	3 cut(s)	815, 976, 3139
BstMAI	GTCTC	3 cut(s)	1030, 2636, 2975
BstMWI	GCNNNNNNNGC	9 cut(s)	404, 1461, 1470, 1961, 2012, 2018, 2288, 2694, 3248
BstNI	CCWGG	4 cut(s)	149, 522, 2508, 3434
BstNSI	RCATGY	7 cut(s)	319, 980, 1468, 3137, 3259, 3375, 3420
BstPAI	GACNNNNGTC	2 cut(s)	1346, 1535
BstSCI	CCNGG	5 cut(s)	147, 520, 1209, 2506, 3432
BstSFI	CTRYAG	2 cut(s)	87, 2874
BstSLI	GKGCMC	1 cut(s)	512
BstUI	CGCG	3 cut(s)	815, 3139, 3463
BstV1I	GCAGC	7 cut(s)	331, 654, 1611, 1704, 1783, 2024, 2357
BstX2I	RGATCY	2 cut(s)	799, 1597
BstYI	RGATCY	2 cut(s)	799, 1597
Bsu15I	ATCGAT	1 cut(s)	712
Bsu36I	CCTNAGG	1 cut(s)	242
BsuI	GTATCC	4 cut(s)	521, 2217, 2246, 2303
BsuRI	GGCC	9 cut(s)	1172, 1435, 1560, 1687, 2367, 2457, 2612, 2688, 3088
BsuTUI	ATCGAT	1 cut(s)	712
BtgZI	GCGATG	1 cut(s)	1085
BveI	ACCTGC	1 cut(s)	2261
Cac8I	GCNNGC	7 cut(s)	1216, 1466, 3059, 3135, 3253, 3418, 3422
CaiI	CAGNNNCTG	3 cut(s)	989, 2291, 2482
CciI	TCATGA	3 cut(s)	2295, 2841, 3276
CfoI	GCGC	3 cut(s)	815, 976, 3139
Cfr13I	GGNCC	7 cut(s)	226, 1433, 1559, 2456, 2630, 3086, 3149
ClaI	ATCGAT	1 cut(s)	712
CseI	GACGC	3 cut(s)	804, 2653, 3452
Csp6I	GTAC	7 cut(s)	782, 980, 1693, 1927, 2421, 3073, 3366
CviQI	GTAC	7 cut(s)	782, 980, 1693, 1927, 2421, 3073, 3366
EaeI	YGGCCR	2 cut(s)	2365, 2610
Eam1104I	CTCTTC	4 cut(s)	477, 934, 1137, 1545
EarI	CTCTTC	4 cut(s)	477, 934, 1137, 1545
Ecl136II	GAGCTC	1 cut(s)	944
Eco130I	CCWWGG	2 cut(s)	2349, 2896
Eco24I	GRGCYC	1 cut(s)	946
Eco32I	GATATC	1 cut(s)	3282
Eco47I	GGWCC	3 cut(s)	226, 2630, 3149
Eco53kI	GAGCTC	1 cut(s)	944
Eco57I	CTGAAG	2 cut(s)	2655, 3201
Eco72I	CACGTG	1 cut(s)	3155
Eco81I	CCTNAGG	1 cut(s)	242
EcoICRI	GAGCTC	1 cut(s)	944
EcoNI	CCTNNNNNAGG	1 cut(s)	3180
EcoO109I	RGGNCCY	1 cut(s)	2630
EcoRII	CCWGG	4 cut(s)	147, 520, 2506, 3432
EcoRV	GATATC	1 cut(s)	3282
EcoT14I	CCWWGG	2 cut(s)	2349, 2896
EcoT22I	ATGCAT	4 cut(s)	631, 1329, 1957, 3135
EcoT38I	GRGCYC	1 cut(s)	946
ErhI	CCWWGG	2 cut(s)	2349, 2896
Esp3I	CGTCTC	2 cut(s)	1030, 2636
FalI	AAGNNNNNCTT	6 cut(s)	474, 506, 561, 593, 1348, 1380
FaqI	GGGAC	3 cut(s)	18, 2386, 2537
FauI	CCCGC	1 cut(s)	1797
FauNDI	CATATG	1 cut(s)	340
FbaI	TGATCA	3 cut(s)	10, 40, 2838
FblI	GTMKAC	2 cut(s)	83, 2725
FriOI	GRGCYC	1 cut(s)	946
FspBI	CTAG	5 cut(s)	1215, 1277, 1437, 2109, 3285
FspI	TGCGCA	1 cut(s)	975
GlaI	GCGC	3 cut(s)	814, 975, 3138
GsaI	CCCAGC	2 cut(s)	1455, 2913
GsuI	CTGGAG	3 cut(s)	543, 2490, 3425
HaeIII	GGCC	9 cut(s)	1172, 1435, 1560, 1687, 2367, 2457, 2612, 2688, 3088
HapII	CCGG	4 cut(s)	1210, 1634, 2176, 2609
HgaI	GACGC	3 cut(s)	804, 2653, 3452
HhaI	GCGC	3 cut(s)	815, 976, 3139
Hin6I	GCGC	3 cut(s)	813, 974, 3137
HinP1I	GCGC	3 cut(s)	813, 974, 3137
HincII	GTYRAC	2 cut(s)	2226, 2726
HindII	GTYRAC	2 cut(s)	2226, 2726
HindIII	AAGCTT	2 cut(s)	62, 3347
HinfI	GANTC	8 cut(s)	152, 181, 602, 727, 1496, 1531, 1637, 2845
HpaII	CCGG	4 cut(s)	1210, 1634, 2176, 2609
HphI	GGTGA	7 cut(s)	136, 278, 515, 910, 2044, 2084, 2097
Hpy99I	CGWCG	1 cut(s)	2727
HpyCH4IV	ACGT	4 cut(s)	285, 1020, 1785, 3154
HpyF10VI	GCNNNNNNNGC	9 cut(s)	404, 1461, 1470, 1961, 2012, 2018, 2288, 2694, 3248
HpySE526I	ACGT	4 cut(s)	285, 1020, 1785, 3154
HspAI	GCGC	3 cut(s)	813, 974, 3137
Kpn2I	TCCGGA	1 cut(s)	1633
Ksp22I	TGATCA	3 cut(s)	10, 40, 2838
LguI	GCTCTTC	1 cut(s)	934
LmnI	GCTCC	7 cut(s)	76, 352, 1374, 1621, 2003, 2267, 3248
Lsp1109I	GCAGC	7 cut(s)	331, 654, 1611, 1704, 1783, 2024, 2357
LweI	GCATC	9 cut(s)	423, 638, 1057, 1149, 2314, 2452, 2463, 2706, 3120
MaeI	CTAG	5 cut(s)	1215, 1277, 1437, 2109, 3285
MaeII	ACGT	4 cut(s)	285, 1020, 1785, 3154
MaeIII	GTNAC	9 cut(s)	634, 816, 1094, 1256, 1537, 1591, 1910, 2103, 2566
MbiI	CCGCTC	1 cut(s)	3271
MfeI	CAATTG	1 cut(s)	2238
MflI	RGATCY	2 cut(s)	799, 1597
MhlI	GDGCHC	2 cut(s)	512, 946
MlyI	GAGTC	2 cut(s)	1525, 1631
MmeI	TCCRAC	3 cut(s)	772, 1086, 2230
Mph1103I	ATGCAT	4 cut(s)	631, 1329, 1957, 3135
MroI	TCCGGA	1 cut(s)	1633
MroXI	GAANNNNTTC	3 cut(s)	372, 1288, 2802
MslI	CAYNNNNRTG	5 cut(s)	650, 2122, 2378, 3080, 3415
MspA1I	CMGCKG	2 cut(s)	459, 2370
MspCI	CTTAAG	1 cut(s)	3350
MspI	CCGG	4 cut(s)	1210, 1634, 2176, 2609
MspR9I	CCNGG	5 cut(s)	149, 522, 1211, 2508, 3434
MunI	CAATTG	1 cut(s)	2238
Mva1269I	GAATGC	1 cut(s)	1329
MvaI	CCWGG	4 cut(s)	149, 522, 2508, 3434
MvnI	CGCG	3 cut(s)	815, 3139, 3463
MwoI	GCNNNNNNNGC	9 cut(s)	404, 1461, 1470, 1961, 2012, 2018, 2288, 2694, 3248
NciI	CCSGG	1 cut(s)	1211
NdeI	CATATG	1 cut(s)	340
NheI	GCTAGC	1 cut(s)	1214
NlaIV	GGNNCC	4 cut(s)	228, 801, 3087, 3250
NmuCI	GTSAC	5 cut(s)	634, 816, 1537, 1910, 2103
NsbI	TGCGCA	1 cut(s)	975
NsiI	ATGCAT	4 cut(s)	631, 1329, 1957, 3135
NspI	RCATGY	7 cut(s)	319, 980, 1468, 3137, 3259, 3375, 3420
NspV	TTCGAA	2 cut(s)	581, 3297
PaeI	GCATGC	3 cut(s)	1468, 3137, 3420
PagI	TCATGA	3 cut(s)	2295, 2841, 3276
PciI	ACATGT	2 cut(s)	315, 3371
PciSI	GCTCTTC	1 cut(s)	934
PcsI	WCGNNNNNNNCGW	1 cut(s)	2719
PctI	GAATGC	1 cut(s)	1329
PdmI	GAANNNNTTC	3 cut(s)	372, 1288, 2802
PfeI	GAWTC	6 cut(s)	152, 181, 602, 727, 1496, 2845
PflMI	CCANNNNNTGG	2 cut(s)	1458, 2735
PfoI	TCCNGGA	2 cut(s)	520, 3432
PleI	GAGTC	2 cut(s)	1525, 1631
PmaCI	CACGTG	1 cut(s)	3155
PmlI	CACGTG	1 cut(s)	3155
PpsI	GAGTC	2 cut(s)	1525, 1631
Ppu21I	YACGTR	1 cut(s)	3155
PpuMI	RGGWCCY	1 cut(s)	2630
PscI	ACATGT	2 cut(s)	315, 3371
PshAI	GACNNNNGTC	2 cut(s)	1346, 1535
PshBI	ATTAAT	1 cut(s)	306
PsiI	TTATAA	2 cut(s)	281, 737
Psp124BI	GAGCTC	1 cut(s)	946
Psp1406I	AACGTT	1 cut(s)	285
Psp5II	RGGWCCY	1 cut(s)	2630
Psp6I	CCWGG	4 cut(s)	147, 520, 2506, 3432
PspCI	CACGTG	1 cut(s)	3155
PspFI	CCCAGC	2 cut(s)	1451, 2909
PspGI	CCWGG	4 cut(s)	147, 520, 2506, 3432
PspN4I	GGNNCC	4 cut(s)	228, 801, 3087, 3250
PspPI	GGNCC	7 cut(s)	226, 1433, 1559, 2456, 2630, 3086, 3149
PspPPI	RGGWCCY	1 cut(s)	2630
PstI	CTGCAG	1 cut(s)	91
PstNI	CAGNNNCTG	3 cut(s)	989, 2291, 2482
PsuI	RGATCY	2 cut(s)	799, 1597
RsaI	GTAC	7 cut(s)	783, 981, 1694, 1928, 2422, 3074, 3367
RsaNI	GTAC	7 cut(s)	782, 980, 1693, 1927, 2421, 3073, 3366
RseI	CAYNNNNRTG	5 cut(s)	650, 2122, 2378, 3080, 3415
SacI	GAGCTC	1 cut(s)	946
SalI	GTCGAC	1 cut(s)	2724
SapI	GCTCTTC	1 cut(s)	934
Sau96I	GGNCC	7 cut(s)	226, 1433, 1559, 2456, 2630, 3086, 3149
ScaI	AGTACT	1 cut(s)	1694
SchI	GAGTC	2 cut(s)	1525, 1631
ScrFI	CCNGG	5 cut(s)	149, 522, 1211, 2508, 3434
SduI	GDGCHC	2 cut(s)	512, 946
SfaNI	GCATC	9 cut(s)	423, 638, 1057, 1149, 2314, 2452, 2463, 2706, 3120
SfcI	CTRYAG	2 cut(s)	87, 2874
SfuI	TTCGAA	2 cut(s)	581, 3297
SinI	GGWCC	3 cut(s)	226, 2630, 3149
SmiMI	CAYNNNNRTG	5 cut(s)	650, 2122, 2378, 3080, 3415
SmlI	CTYRAG	2 cut(s)	887, 3350
SmoI	CTYRAG	2 cut(s)	887, 3350
SphI	GCATGC	3 cut(s)	1468, 3137, 3420
SsiI	CCGC	8 cut(s)	311, 448, 457, 1102, 1804, 2368, 3271, 3461
SspI	AATATT	3 cut(s)	304, 354, 2155
SspMI	CTAG	5 cut(s)	1215, 1277, 1437, 2109, 3285
SstI	GAGCTC	1 cut(s)	946
StyD4I	CCNGG	5 cut(s)	147, 520, 1209, 2506, 3432
StyI	CCWWGG	2 cut(s)	2349, 2896
TaiI	ACGT	4 cut(s)	288, 1023, 1788, 3157
TaqI	TCGA	7 cut(s)	581, 712, 1305, 2655, 2725, 3297, 3446
TaqII	GACCGA	1 cut(s)	2539
TatI	WGTACW	4 cut(s)	781, 1692, 2420, 3365
TauI	GCSGC	3 cut(s)	459, 2370, 3274
TfiI	GAWTC	6 cut(s)	152, 181, 602, 727, 1496, 2845
TseFI	GTSAC	5 cut(s)	634, 816, 1537, 1910, 2103
TseI	GCWGC	7 cut(s)	344, 642, 1624, 1717, 1771, 2012, 2370
Tsp45I	GTSAC	5 cut(s)	634, 816, 1537, 1910, 2103
TspGWI	ACGGA	5 cut(s)	393, 622, 930, 951, 2003
Van91I	CCANNNNNTGG	2 cut(s)	1458, 2735
Vha464I	CTTAAG	1 cut(s)	3350
VpaK11BI	GGWCC	3 cut(s)	226, 2630, 3149
VspI	ATTAAT	1 cut(s)	306
XagI	CCTNNNNNAGG	1 cut(s)	3180
XapI	RAATTY	4 cut(s)	95, 1395, 2744, 2823
XceI	RCATGY	7 cut(s)	319, 980, 1468, 3137, 3259, 3375, 3420
XmiI	GTMKAC	2 cut(s)	83, 2725
XmnI	GAANNNNTTC	3 cut(s)	372, 1288, 2802
XspI	CTAG	5 cut(s)	1215, 1277, 1437, 2109, 3285
ZrmI	AGTACT	1 cut(s)	1694
Zsp2I	ATGCAT	4 cut(s)	631, 1329, 1957, 3135

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.