Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003584

Physical Location & Seq

Genomic Coordinates Reverse (-)

2570947 .. 2575160

Gene Structure 4214 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1029000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3495 bp

ATGGGCGTCGCCCTGGACTCTTCCGTCGACCCTCCTGGCAACGACAAGCAACGGTTTACAAATGCAAGGCAAGCTCCTCAGTCAGCCATTGATATGGAATTCCTCGCCGCTCCTATCAAATCCGCCAAGGACAAATTCGCGCTCGTCCCGGAATTTCTGAAGGTGAGGGGGTTGGTAAAGCAGCACTTAGACTCGTTTAATTACTTTGTGAACACGGGAATCAAGAAAATTGTTCGTGCCAATAATCGGATTGAGTCGGAGATCGATCCAACTATTTATCTCGAGTTCAAAGATATCTATGTCGATAAGGCCTCGGTGATGTCAATGGGTAAAACTGATGATATTTCTCCCAATACATGCCGCTTATCTGATATGACGTATGCTGCACCGATAAAGGTTGATATAGTGTACATTCGAGGAAGTCATGATCGGAAGGAGAAAGTTGAGAGGAAAGGTGTGACTATTGGGAGAATGCCTATTATGTTACAGAGTTGCTGCTGTACTCTACTGGGGAAAAATGAAGCCGAGCTTGCAAAACTGGGCGAGTGCCCCCTGGATCCTGGAGGATATTTTATCATCAAAGGGAATGAGAAGGTGCTTTTGATGCAGGAACAACTCTCAAAAAACAGGATTATCATTGATATGGACAAGAAAGGGAACTTTAATGCATCTGTTACAAGCAGCTCAGAGAGAATCAAAAGTAAAACCGTTATTCAGATGGAAAATCAGAGGATCTATTTGATACTTAACCAATTTGCCAAGAAGATTCCTATTATGGTGGTCATGAAGGCTTTGGGAATGACGGGTGATCAAGAAGTTGTGCAGATGGTTGGTAGAGATCCGCGGTATGGTGCCTTGCTAATGCCATCTTTGGAGGATTGTGCAAAGGAAGGTATATACACACAAGAGCAAGCCTTGGACTACCTTGAGTCAAAGCTGAAGCGGTTTAATTTTTCTGGTTCTTCATCCGAGAAGGATAGTGCTAGAGTGATGAAACTTCTTCGAGATGTGTTTCTTGCAAATGTTATGTGTCAGAATGATTTTCGTCCTAAATGTGTATATGTTGCTGTAATGTTGAGACGCATAATGGATGCAGTTTTAAATAAAGATGCAATGGATGACAAGGATTATGTTGGTAACAAGCGGATTGAGTTGTCTGGTCAGTTAATCTCTCTTCTATTTGAGGATCTGTTCAAGACGATGATTACTGATATTAAAAAGATAGGAGACAATATATTATCAAAGCGTAGCAGGAGTAGCGCTTTTGACTTTGCACAGCAGCTTACGAGTGATAGAATTACAAATGGGCTAGAAAGGACCTTATCCACCGGAAACTTCAACATAAAACGGTTCAAAATGGACAGTAAAGGTATGTCACAGGTCCTATTTAGGTTATCCTACATAGGGTGTCTGGGCCATATGACCAGAGTCAAGCCACAATTTGAGAAGACACGAAAAGTAAGTGGACCTAGAGCCTTGCAACCTAGCCAGTGGGGAATGCTTTGCCCTTGTGACACTCCAGAAGGTGAGCAATGTGGACTGGTTAAAAACTTGTCTTTGATGGCTCATGTGACAACTGATGAAGAGGAGGGCCCATTAATGTCTCTGTGCAAGTGTTTGGGTATTGAAGGCCTGGAAATACTATCAGCCGAGGAGGTTCACTCACCATATTCTTTTCTGGTTTTCTTCAATGGAAACATCCTTGGAAAGCATAAGAAGCCACATCACTTTGCCAATGCATTGAGAAAGTTGCGTAGAGCTGGAAAGATTGGGGAGTTTGTAAGTGTCTTCGTCAATGAGAAACAGCGTAGTGTTTACATTGCCTCTGATGGTGGTCGAGTTTGTCGTCCACTAGTTATTGCTGACAAGGGCGTACCAAGGGTCAAAGAACATCATATGCAGGAGTTGAAGGATGGAGTACGCACTTTTAATGATTTTTTAACTGATGGGCTGATTGAGTATCTTGATGTTAATGAGGAGAACAATGCTCTGATTGCTCTATATGAAAATGAGGCGACAACAGAAACAACCCATATTGAAATAGAGCCTTTCACCATCTTAGGTGTTATTGCTGGGCTAATTCCATATCCTCATCACAATCAGTCGCCTAGAAATACGTACCAGTGTGCTATGGGAAAGCAAGCAATGGGAAATATTGCTTATAATCAGCTACAAAGAATGGACATGCTTTACCTTCTGGTGTATCCTCAACGGCCATTATTGACAACGAAGACAATTGAGTTGGTGGGCTTTGATAAACTTGGAGCCGGGCAAAATGCAACTGTTGCTGTGATGAGCTATAGTGGCTATGACATAGAGGATGCAATAGTCATGAACAAGGCATCTCTGGATCGTGGTTTTGGTCGCTGTATTCTAATGAAAAAATTTTCTGGCACGCTTCAAAAATATGAAAATGATGCACGCGACAGAATAGCAAGGCCCTATAGGGATGGAACTGATGCTGGAAGGATGCGGGCGCTGGACAATGATGGACTTGCTGCACCTGGAGAAATTATTAGAAATAATGACATTCTAATGAATAAACAGGTTCCTCAAGTGACGAGGGGGCCGCAGATTTCCCCAAGCCAACTAAATATTCCATATGGAAATGCTCCAGTGTCTTACAAGGGACCTCATGGAGAATCTTGTGTGGTTGACAAAGTGACCCTCTCTTCTGATAAACATAATAATTTATGTTCCAAGTTCTTAATTCGCCATACTCGTAGACCAGAAGTTGGTGACAAGTTCAGTAGCAGACATGGGCAGAAAGGTGTTTGTGGTACCATTGTCCAGCAGGAAGATTTCCCTTTTTCAGAACGTGGCATTTGCCCTGATTTAATTATGAACCCTCATGGGTTTCCAAGTCGAATGACTGTAGGCAAGATGATAGAACTTCTTGGGAGCAAAGCAGGAGTGTCATGTGGCAGGTTTCATTATGGCAGTGCCTTTGGTGAACCAAGTGGTCATGCAGACAAGGTTGAAGATATAAGTGAAACGCTTGTGAGGATGGGGTTTAGCTACGACGGCAAGGACTTGATTTACTCAGGTATCACAGGCTGCCCTTTACAGGCATATATATTCATGGGACCAATTTACTACCAGAAGTTAAAGCATATGGTTCTAGATAAAATGCATGCACGGGGTAGTGGTCCTCGAGTCAGTCTAACTAGGCAACCTACCGAGGGAAAAGCCCGTAATGGAGGACTACGAGTTGGAGAAATGGAGCGTGATTGTTTGATTGCTTATGGTGCTAGTATGCTGCTTTATGAGCGCCTGATGCTGTCGAGTGATCCTTTTGAAGTTCATGTTTGCAAAGAGTGTGGTTTGTTGGGATATTACAGCCATAAGCTGAAAACTGGAATTTGTTCCTCGTGCAAAACTGGGGATAAAATGTATACTGCAAAGATGCCGTATGCTTGCAAGCTCTTGATTCAAGAACTCCAGTCGATGAACATTGTTCCTCGCATAAAACCGGAAGAGCTTGAAAAGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006383 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032501 GO:0032502 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045089 GO:0046483 GO:0048229 GO:0048518 GO:0048583 GO:0048584 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080134 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1165

Amino Acids

130.35

Weight (kDa)

8.81

Isoelectric Point (pI)

41.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	57 - 433	5.2e-52	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	208 - 384	6.3e-23	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	460 - 524	1.1e-19	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	561 - 621	1.6e-24	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_6	PF00562	690 - 1065	1.2e-132	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1067 - 1157	5.6e-30	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45140 AT5G45140 AT5G45140
fragaria_vesca	FvH4_5g31720 FvH4_7g03930
malus_domestica	MD14G1029000.v1.1
prunus_persica	Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1
pyrus_communis	pycom14g02710
rosa_chinensis	RchiOBHm_Chr1g0327591 RchiOBHm_Chr7g0227611
rosa_laevigata	RLG00000001632 RLG00000029981
rosa_multiflora	Rmu_co8491793.1_g000001 Rmu_sc0001772.1_g000005 Rmu_ssc0000042.1_g000027
rosa_roxburghii	Rroxscaffold_3G00232000 Rroxscaffold_4G00322550
rosa_rugosa	Rorug01G0068500 Rorug07G0242400
rosa_samantha	Rh1AG085800 Rh1BG068400 Rh1CG083300 Rh1DG090000 Rh7AG395800 Rh7BG377500 Rh7CG414500 Rh7DG391600
rosa_wichuraiana	Rw1G006690 Rw7G032920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2163
AasI	GACNNNNNNGTC	1 cut(s)	23
Acc36I	ACCTGC	1 cut(s)	2916
Acc65I	GGTACC	1 cut(s)	2780
AccB1I	GGYRCC	2 cut(s)	851, 2780
AccB7I	CCANNNNNTGG	1 cut(s)	2735
AccBSI	CCGCTC	1 cut(s)	110
AccI	GTMKAC	3 cut(s)	27, 2725, 3395
AccII	CGCG	3 cut(s)	140, 844, 2424
AciI	CCGC	9 cut(s)	108, 123, 361, 842, 844, 943, 1144, 2473, 2570
AclWI	GGATC	8 cut(s)	260, 551, 564, 740, 833, 1194, 2358, 3284
AcoI	YGGCCR	1 cut(s)	2213
AcsI	RAATTY	5 cut(s)	98, 134, 152, 2384, 3360
AcuI	CTGAAG	2 cut(s)	179, 959
AcyI	GRCGYC	1 cut(s)	6
AfaI	GTAC	6 cut(s)	410, 502, 1875, 1920, 2120, 2782
AfeI	AGCGCT	1 cut(s)	1261
AfiI	CCNNNNNNNGG	6 cut(s)	246, 848, 1404, 2735, 3067, 3182
AhlI	ACTAGT	1 cut(s)	1852
AjnI	CCWGG	6 cut(s)	12, 34, 552, 559, 1632, 2503
AjuI	GAANNNNNNNTTGG	4 cut(s)	119, 151, 2580, 2612
Alw26I	GTCTC	3 cut(s)	1072, 1221, 1608
AlwI	GGATC	8 cut(s)	260, 551, 564, 740, 833, 1194, 2358, 3284
Ama87I	CYCGRG	2 cut(s)	281, 3153
Aor51HI	AGCGCT	1 cut(s)	1261
AoxI	GGCC	7 cut(s)	309, 1414, 1591, 1630, 2213, 2438, 2567
ApaI	GGGCCC	1 cut(s)	1595
ApeKI	GCWGC	8 cut(s)	181, 383, 495, 681, 1279, 2498, 3057, 3259
ApoI	RAATTY	5 cut(s)	98, 134, 152, 2384, 3360
AseI	ATTAAT	1 cut(s)	1598
Asp700I	GAANNNNTTC	1 cut(s)	3364
Asp718I	GGTACC	1 cut(s)	2780
AspLEI	GCGC	4 cut(s)	142, 1262, 2479, 3273
AsuC2I	CCSGG	2 cut(s)	149, 2269
AsuHPI	GGTGA	8 cut(s)	175, 328, 818, 1538, 1656, 2044, 2750, 2963
AvaI	CYCGRG	2 cut(s)	281, 3153
AvaII	GGWCC	6 cut(s)	1317, 1381, 1466, 2630, 3086, 3149
BaeGI	GKGCMC	2 cut(s)	551, 1595
BaeI	ACNNNNGTAYC	4 cut(s)	2772, 2805, 3031, 3064
BamHI	GGATCC	1 cut(s)	556
BanI	GGYRCC	2 cut(s)	851, 2780
BanII	GRGCYC	1 cut(s)	1595
BauI	CACGAG	1 cut(s)	3370
BbsI	GAAGAC	3 cut(s)	1454, 1780, 2237
BbvI	GCAGC	8 cut(s)	193, 370, 482, 693, 1291, 2485, 3044, 3246
BceAI	ACGGC	3 cut(s)	2228, 3040, 3394
BciT130I	CCWGG	6 cut(s)	14, 36, 554, 561, 1634, 2505
BciVI	GTATCC	1 cut(s)	2214
BclI	TGATCA	1 cut(s)	808
BcnI	CCSGG	2 cut(s)	149, 2269
BcoDI	GTCTC	3 cut(s)	1072, 1221, 1608
BcuI	ACTAGT	1 cut(s)	1852
BfaI	CTAG	9 cut(s)	984, 1310, 1470, 1485, 1853, 2109, 3122, 3168, 3252
BfmI	CTRYAG	3 cut(s)	2299, 2443, 2874
BfoI	RGCGCY	3 cut(s)	1263, 2480, 3274
BfuAI	ACCTGC	1 cut(s)	2916
BfuI	GTATCC	1 cut(s)	2214
Bme1390I	CCNGG	8 cut(s)	14, 36, 149, 554, 561, 1634, 2269, 2505
Bme18I	GGWCC	6 cut(s)	1317, 1381, 1466, 2630, 3086, 3149
BmeT110I	CYCGRG	2 cut(s)	281, 3153
BmiI	GGNNCC	9 cut(s)	558, 853, 1593, 2266, 2550, 2568, 2631, 2782, 3087
BmrFI	CCNGG	8 cut(s)	14, 36, 149, 554, 561, 1634, 2269, 2505
BmrI	ACTGGG	3 cut(s)	518, 548, 3390
BmuI	ACTGGG	3 cut(s)	518, 548, 3390
BoxI	GACNNNNGTC	1 cut(s)	1427
BpiI	GAAGAC	3 cut(s)	1454, 1780, 2237
BplI	GAGNNNNNCTC	2 cut(s)	1646, 1678
BpmI	CTGGAG	5 cut(s)	582, 1503, 2526, 2598, 3425
BpuEI	CTTGAG	2 cut(s)	947, 2538
BpuMI	CCSGG	2 cut(s)	149, 2269
Bsa29I	ATCGAT	1 cut(s)	264
BsaAI	YACGTR	1 cut(s)	2118
BsaHI	GRCGYC	1 cut(s)	6
BsaWI	WCCGGW	2 cut(s)	1328, 3472
Bsc4I	CCNNNNNNNGG	6 cut(s)	246, 848, 1404, 2735, 3067, 3182
Bse1I	ACTGG	9 cut(s)	513, 543, 1489, 1545, 2122, 2615, 3361, 3385, 3442
Bse3DI	GCAATG	4 cut(s)	1119, 1538, 1818, 2151
BseBI	CCWGG	6 cut(s)	14, 36, 554, 561, 1634, 2505
BseCI	ATCGAT	1 cut(s)	264
BseGI	GGATG	9 cut(s)	965, 1096, 1123, 1698, 1918, 2326, 2455, 2475, 3012
BseLI	CCNNNNNNNGG	6 cut(s)	246, 848, 1404, 2735, 3067, 3182
BseMI	GCAATG	4 cut(s)	1119, 1538, 1818, 2151
BseMII	CTCAG	3 cut(s)	92, 699, 3057
BseNI	ACTGG	9 cut(s)	513, 543, 1489, 1545, 2122, 2615, 3361, 3385, 3442
BseRI	GAGGAG	4 cut(s)	66, 1601, 1667, 1991
BseSI	GKGCMC	2 cut(s)	551, 1595
BseXI	GCAGC	8 cut(s)	193, 370, 482, 693, 1291, 2485, 3044, 3246
BseYI	CCCAGC	1 cut(s)	2072
BsgI	GTGCAG	3 cut(s)	369, 842, 2484
Bsh1236I	CGCG	3 cut(s)	140, 844, 2424
BshFI	GGCC	7 cut(s)	311, 1416, 1593, 1632, 2215, 2440, 2569
BshNI	GGYRCC	2 cut(s)	851, 2780
BshVI	ATCGAT	1 cut(s)	264
BsiHKCI	CYCGRG	2 cut(s)	281, 3153
BsiSI	CCGG	4 cut(s)	149, 1329, 2268, 3473
BslFI	GGGAC	3 cut(s)	131, 2643, 3099
BslI	CCNNNNNNNGG	6 cut(s)	246, 848, 1404, 2735, 3067, 3182
BsmAI	GTCTC	3 cut(s)	1072, 1221, 1608
BsmBI	CGTCTC	1 cut(s)	1072
BsmFI	GGGAC	3 cut(s)	131, 2643, 3099
BsmI	GAATGC	2 cut(s)	477, 1503
BsnI	GGCC	7 cut(s)	311, 1416, 1593, 1632, 2215, 2440, 2569
BsoBI	CYCGRG	2 cut(s)	281, 3153
Bsp120I	GGGCCC	1 cut(s)	1591
Bsp1286I	GDGCHC	2 cut(s)	551, 1595
Bsp1407I	TGTACA	1 cut(s)	408
BspACI	CCGC	9 cut(s)	108, 123, 361, 842, 844, 943, 1144, 2473, 2570
BspANI	GGCC	7 cut(s)	311, 1416, 1593, 1632, 2215, 2440, 2569
BspCNI	CTCAG	3 cut(s)	91, 698, 3056
BspDI	ATCGAT	1 cut(s)	264
BspFNI	CGCG	3 cut(s)	140, 844, 2424
BspHI	TCATGA	3 cut(s)	424, 783, 2331
BspLI	GGNNCC	9 cut(s)	558, 853, 1593, 2266, 2550, 2568, 2631, 2782, 3087
BspMI	ACCTGC	1 cut(s)	2916
BspPI	GGATC	8 cut(s)	260, 551, 564, 740, 833, 1194, 2358, 3284
BspQI	GCTCTTC	1 cut(s)	3471
BspT107I	GGYRCC	2 cut(s)	851, 2780
BsrBI	CCGCTC	1 cut(s)	110
BsrDI	GCAATG	4 cut(s)	1119, 1538, 1818, 2151
BsrGI	TGTACA	1 cut(s)	408
BsrI	ACTGG	9 cut(s)	513, 543, 1489, 1545, 2122, 2615, 3361, 3385, 3442
BssNAI	GTATAC	1 cut(s)	3396
BssNI	GRCGYC	1 cut(s)	6
BssSI	CACGAG	1 cut(s)	3370
BssT1I	CCWWGG	4 cut(s)	126, 915, 1702, 1877
Bst1107I	GTATAC	1 cut(s)	3396
Bst2BI	CACGAG	1 cut(s)	3370
Bst2UI	CCWGG	6 cut(s)	14, 36, 554, 561, 1634, 2505
Bst4CI	ACNGT	6 cut(s)	54, 709, 1350, 1364, 2284, 2875
Bst6I	CTCTTC	5 cut(s)	25, 1179, 1578, 2677, 3471
BstACI	GRCGYC	1 cut(s)	6
BstAPI	GCANNNNNTGC	1 cut(s)	2285
BstAUI	TGTACA	1 cut(s)	408
BstBAI	YACGTR	1 cut(s)	2118
BstDEI	CTNAG	5 cut(s)	78, 187, 685, 2059, 3043
BstDSI	CCRYGG	1 cut(s)	842
BstF5I	GGATG	9 cut(s)	965, 1096, 1123, 1698, 1918, 2326, 2455, 2475, 3012
BstFNI	CGCG	3 cut(s)	140, 844, 2424
BstH2I	RGCGCY	3 cut(s)	1263, 2480, 3274
BstHHI	GCGC	4 cut(s)	142, 1262, 2479, 3273
BstMAI	GTCTC	3 cut(s)	1072, 1221, 1608
BstNI	CCWGG	6 cut(s)	14, 36, 554, 561, 1634, 2505
BstNSI	RCATGY	3 cut(s)	360, 2188, 3137
BstPAI	GACNNNNGTC	1 cut(s)	1427
BstSCI	CCNGG	8 cut(s)	12, 34, 147, 552, 559, 1632, 2267, 2503
BstSFI	CTRYAG	3 cut(s)	2299, 2443, 2874
BstSLI	GKGCMC	2 cut(s)	551, 1595
BstSNI	TACGTA	1 cut(s)	2118
BstUI	CGCG	3 cut(s)	140, 844, 2424
BstV1I	GCAGC	8 cut(s)	193, 370, 482, 693, 1291, 2485, 3044, 3246
BstV2I	GAAGAC	3 cut(s)	1454, 1780, 2237
BstX2I	RGATCY	4 cut(s)	556, 732, 838, 1186
BstXI	CCANNNNNNTGG	1 cut(s)	94
BstYI	RGATCY	4 cut(s)	556, 732, 838, 1186
BstZ17I	GTATAC	1 cut(s)	3396
Bsu15I	ATCGAT	1 cut(s)	264
BsuI	GTATCC	1 cut(s)	2214
BsuRI	GGCC	7 cut(s)	311, 1416, 1593, 1632, 2215, 2440, 2569
BsuTUI	ATCGAT	1 cut(s)	264
BtgI	CCRYGG	1 cut(s)	842
BtsCI	GGATG	9 cut(s)	965, 1096, 1123, 1698, 1918, 2326, 2455, 2475, 3012
BtsI	GCAGTG	1 cut(s)	2947
BtsIMutI	CAGTG	4 cut(s)	1496, 2129, 2622, 2947
BveI	ACCTGC	1 cut(s)	2916
CciI	TCATGA	3 cut(s)	424, 783, 2331
CfoI	GCGC	4 cut(s)	142, 1262, 2479, 3273
Cfr42I	CCGCGG	1 cut(s)	845
ClaI	ATCGAT	1 cut(s)	264
CseI	GACGC	1 cut(s)	1089
Csp6I	GTAC	6 cut(s)	409, 501, 1874, 1919, 2119, 2781
CspCI	CAANNNNNGTGG	2 cut(s)	3301, 3336
CviQI	GTAC	6 cut(s)	409, 501, 1874, 1919, 2119, 2781
DdeI	CTNAG	5 cut(s)	78, 187, 685, 2059, 3043
DraI	TTTAAA	1 cut(s)	1101
DrdI	GACNNNNNNGTC	1 cut(s)	23
DseDI	GACNNNNNNGTC	1 cut(s)	23
EaeI	YGGCCR	1 cut(s)	2213
Eam1104I	CTCTTC	5 cut(s)	25, 1179, 1578, 2677, 3471
EarI	CTCTTC	5 cut(s)	25, 1179, 1578, 2677, 3471
EciI	GGCGGA	1 cut(s)	112
Eco105I	TACGTA	1 cut(s)	2118
Eco130I	CCWWGG	4 cut(s)	126, 915, 1702, 1877
Eco147I	AGGCCT	2 cut(s)	311, 1632
Eco24I	GRGCYC	1 cut(s)	1595
Eco32I	GATATC	1 cut(s)	295
Eco47I	GGWCC	6 cut(s)	1317, 1381, 1466, 2630, 3086, 3149
Eco47III	AGCGCT	1 cut(s)	1261
Eco57I	CTGAAG	2 cut(s)	179, 959
Eco88I	CYCGRG	2 cut(s)	281, 3153
EcoO109I	RGGNCCY	5 cut(s)	1317, 1381, 1591, 2439, 2630
EcoRI	GAATTC	1 cut(s)	98
EcoRII	CCWGG	6 cut(s)	12, 34, 552, 559, 1632, 2503
EcoRV	GATATC	1 cut(s)	295
EcoT14I	CCWWGG	4 cut(s)	126, 915, 1702, 1877
EcoT22I	ATGCAT	3 cut(s)	670, 1741, 3135
EcoT38I	GRGCYC	1 cut(s)	1595
ErhI	CCWWGG	4 cut(s)	126, 915, 1702, 1877
Esp3I	CGTCTC	1 cut(s)	1072
FalI	AAGNNNNNCTT	4 cut(s)	170, 202, 513, 545
FaqI	GGGAC	3 cut(s)	131, 2643, 3099
FauI	CCCGC	1 cut(s)	2466
FauNDI	CATATG	4 cut(s)	1419, 1896, 2602, 3114
FbaI	TGATCA	1 cut(s)	808
FblI	GTMKAC	3 cut(s)	27, 2725, 3395
FokI	GGATG	9 cut(s)	952, 1103, 1130, 1685, 1925, 2333, 2462, 2482, 3019
FriOI	GRGCYC	1 cut(s)	1595
FspBI	CTAG	9 cut(s)	984, 1310, 1470, 1485, 1853, 2109, 3122, 3168, 3252
GlaI	GCGC	4 cut(s)	141, 1261, 2478, 3272
GsaI	CCCAGC	1 cut(s)	2076
GsuI	CTGGAG	5 cut(s)	582, 1503, 2526, 2598, 3425
HaeII	RGCGCY	3 cut(s)	1263, 2480, 3274
HaeIII	GGCC	7 cut(s)	311, 1416, 1593, 1632, 2215, 2440, 2569
HapII	CCGG	4 cut(s)	149, 1329, 2268, 3473
HgaI	GACGC	1 cut(s)	1089
HhaI	GCGC	4 cut(s)	142, 1262, 2479, 3273
Hin1I	GRCGYC	1 cut(s)	6
Hin6I	GCGC	4 cut(s)	140, 1260, 2477, 3271
HinP1I	GCGC	4 cut(s)	140, 1260, 2477, 3271
HincII	GTYRAC	2 cut(s)	28, 2656
HindII	GTYRAC	2 cut(s)	28, 2656
HpaII	CCGG	4 cut(s)	149, 1329, 2268, 3473
HphI	GGTGA	8 cut(s)	175, 328, 818, 1538, 1656, 2044, 2750, 2963
Hpy99I	CGWCG	3 cut(s)	11, 29, 3026
HpyCH4III	ACNGT	6 cut(s)	54, 709, 1350, 1364, 2284, 2875
HpyCH4IV	ACGT	3 cut(s)	377, 2117, 2818
HpyF3I	CTNAG	5 cut(s)	78, 187, 685, 2059, 3043
HpySE526I	ACGT	3 cut(s)	377, 2117, 2818
Hsp92I	GRCGYC	1 cut(s)	6
HspAI	GCGC	4 cut(s)	140, 1260, 2477, 3271
KpnI	GGTACC	1 cut(s)	2784
Ksp22I	TGATCA	1 cut(s)	808
KspI	CCGCGG	1 cut(s)	845
LguI	GCTCTTC	1 cut(s)	3471
LmnI	GCTCC	6 cut(s)	79, 115, 2264, 2617, 2901, 3223
Lsp1109I	GCAGC	8 cut(s)	193, 370, 482, 693, 1291, 2485, 3044, 3246
MaeI	CTAG	9 cut(s)	984, 1310, 1470, 1485, 1853, 2109, 3122, 3168, 3252
MaeII	ACGT	3 cut(s)	377, 2117, 2818
MbiI	CCGCTC	1 cut(s)	110
MfeI	CAATTG	1 cut(s)	2235
MflI	RGATCY	4 cut(s)	556, 732, 838, 1186
MhlI	GDGCHC	2 cut(s)	551, 1595
MlyI	GAGTC	6 cut(s)	11, 185, 263, 938, 1437, 3165
MmeI	TCCRAC	3 cut(s)	237, 293, 3193
Mph1103I	ATGCAT	3 cut(s)	670, 1741, 3135
MroXI	GAANNNNTTC	1 cut(s)	3364
MslI	CAYNNNNRTG	3 cut(s)	92, 641, 2534
MspA1I	CMGCKG	1 cut(s)	844
MspI	CCGG	4 cut(s)	149, 1329, 2268, 3473
MspR9I	CCNGG	8 cut(s)	14, 36, 149, 554, 561, 1634, 2269, 2505
MunI	CAATTG	1 cut(s)	2235
Mva1269I	GAATGC	2 cut(s)	477, 1503
MvaI	CCWGG	6 cut(s)	14, 36, 554, 561, 1634, 2505
MvnI	CGCG	3 cut(s)	140, 844, 2424
NciI	CCSGG	2 cut(s)	149, 2269
NdeI	CATATG	4 cut(s)	1419, 1896, 2602, 3114
NlaIV	GGNNCC	9 cut(s)	558, 853, 1593, 2266, 2550, 2568, 2631, 2782, 3087
NmeAIII	GCCGAG	2 cut(s)	550, 1675
NmuCI	GTSAC	7 cut(s)	457, 1374, 1511, 1570, 2557, 2662, 2738
NsiI	ATGCAT	3 cut(s)	670, 1741, 3135
NspI	RCATGY	3 cut(s)	360, 2188, 3137
PaeI	GCATGC	1 cut(s)	3137
PaeR7I	CTCGAG	2 cut(s)	281, 3153
PagI	TCATGA	3 cut(s)	424, 783, 2331
PceI	AGGCCT	2 cut(s)	311, 1632
PciSI	GCTCTTC	1 cut(s)	3471
PcsI	WCGNNNNNNNCGW	2 cut(s)	1843, 2719
PctI	GAATGC	2 cut(s)	477, 1503
PdmI	GAANNNNTTC	1 cut(s)	3364
PfeI	GAWTC	5 cut(s)	219, 693, 766, 2642, 3430
PflMI	CCANNNNNTGG	1 cut(s)	2735
PfoI	TCCNGGA	2 cut(s)	147, 559
PleI	GAGTC	6 cut(s)	11, 185, 262, 937, 1436, 3164
PpsI	GAGTC	6 cut(s)	11, 185, 262, 937, 1436, 3164
Ppu21I	YACGTR	1 cut(s)	2118
PpuMI	RGGWCCY	3 cut(s)	1317, 1381, 2630
PshAI	GACNNNNGTC	1 cut(s)	1427
PshBI	ATTAAT	1 cut(s)	1598
PsiI	TTATAA	1 cut(s)	2163
Psp5II	RGGWCCY	3 cut(s)	1317, 1381, 2630
Psp6I	CCWGG	6 cut(s)	12, 34, 552, 559, 1632, 2503
PspFI	CCCAGC	1 cut(s)	2072
PspGI	CCWGG	6 cut(s)	12, 34, 552, 559, 1632, 2503
PspN4I	GGNNCC	9 cut(s)	558, 853, 1593, 2266, 2550, 2568, 2631, 2782, 3087
PspOMI	GGGCCC	1 cut(s)	1591
PspPPI	RGGWCCY	3 cut(s)	1317, 1381, 2630
PspXI	VCTCGAGB	1 cut(s)	3153
PsuI	RGATCY	4 cut(s)	556, 732, 838, 1186
RsaI	GTAC	6 cut(s)	410, 502, 1875, 1920, 2120, 2782
RsaNI	GTAC	6 cut(s)	409, 501, 1874, 1919, 2119, 2781
RseI	CAYNNNNRTG	3 cut(s)	92, 641, 2534
SacII	CCGCGG	1 cut(s)	845
SalI	GTCGAC	1 cut(s)	26
SapI	GCTCTTC	1 cut(s)	3471
SchI	GAGTC	6 cut(s)	11, 185, 263, 938, 1437, 3165
ScrFI	CCNGG	8 cut(s)	14, 36, 149, 554, 561, 1634, 2269, 2505
SduI	GDGCHC	2 cut(s)	551, 1595
SfcI	CTRYAG	3 cut(s)	2299, 2443, 2874
Sfr274I	CTCGAG	2 cut(s)	281, 3153
Sfr303I	CCGCGG	1 cut(s)	845
SgrBI	CCGCGG	1 cut(s)	845
SinI	GGWCC	6 cut(s)	1317, 1381, 1466, 2630, 3086, 3149
SlaI	CTCGAG	2 cut(s)	281, 3153
SmiMI	CAYNNNNRTG	3 cut(s)	92, 641, 2534
SmlI	CTYRAG	4 cut(s)	281, 926, 2553, 3153
SmoI	CTYRAG	4 cut(s)	281, 926, 2553, 3153
SnaBI	TACGTA	1 cut(s)	2118
SpeI	ACTAGT	1 cut(s)	1852
SphI	GCATGC	1 cut(s)	3137
SseBI	AGGCCT	2 cut(s)	311, 1632
SsiI	CCGC	9 cut(s)	108, 123, 361, 842, 844, 943, 1144, 2473, 2570
SspI	AATATT	2 cut(s)	2155, 2596
SspMI	CTAG	9 cut(s)	984, 1310, 1470, 1485, 1853, 2109, 3122, 3168, 3252
StuI	AGGCCT	2 cut(s)	311, 1632
StyD4I	CCNGG	8 cut(s)	12, 34, 147, 552, 559, 1632, 2267, 2503
StyI	CCWWGG	4 cut(s)	126, 915, 1702, 1877
TaaI	ACNGT	6 cut(s)	54, 709, 1350, 1364, 2284, 2875
TaiI	ACGT	3 cut(s)	380, 2120, 2821
TatI	WGTACW	2 cut(s)	408, 500
TauI	GCSGC	3 cut(s)	110, 363, 2572
TfiI	GAWTC	5 cut(s)	219, 693, 766, 2642, 3430
TscAI	CASTG	4 cut(s)	1496, 2129, 2622, 2947
TseFI	GTSAC	7 cut(s)	457, 1374, 1511, 1570, 2557, 2662, 2738
TseI	GCWGC	8 cut(s)	181, 383, 495, 681, 1279, 2498, 3057, 3259
Tsp45I	GTSAC	7 cut(s)	457, 1374, 1511, 1570, 2557, 2662, 2738
TspGWI	ACGGA	1 cut(s)	13
TspRI	CASTG	4 cut(s)	1496, 2129, 2622, 2947
Van91I	CCANNNNNTGG	1 cut(s)	2735
VpaK11BI	GGWCC	6 cut(s)	1317, 1381, 1466, 2630, 3086, 3149
VspI	ATTAAT	1 cut(s)	1598
XapI	RAATTY	5 cut(s)	98, 134, 152, 2384, 3360
XbaI	TCTAGA	1 cut(s)	3121
XceI	RCATGY	3 cut(s)	360, 2188, 3137
XhoI	CTCGAG	2 cut(s)	281, 3153
XmiI	GTMKAC	3 cut(s)	27, 2725, 3395
XmnI	GAANNNNTTC	1 cut(s)	3364
XspI	CTAG	9 cut(s)	984, 1310, 1470, 1485, 1853, 2109, 3122, 3168, 3252
Zsp2I	ATGCAT	3 cut(s)	670, 1741, 3135

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.