Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0003584

Physical Location & Seq

Genomic Coordinates Forward (+)

18535107 .. 18538541

Gene Structure 3435 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000001632

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3435 bp

ATGGGCGTGCCGGTCAAGTCACTGCTGGACCCTAACCCCGGCTCCGCCATCGATAAGCAATTCCTGGCGGCGCCGATCAAATCCGCAACCGATAAATTTGCTCTGCTGCCGGAGCTTCTCAAGGTCAGAGGGATCGTCAGGGAACATCTGGACTCCTTCAACTACTTCGTCAACACCGGAATCAAGAGAATCGTGCGCGCCAATGACCGCATTGTGTCCGCAGTTGATCCCAGAATTTTCATCAAGTTCTTAAATGTAGAGATTGGCGAGGCCTCCGTGACCAGGGATGGTGTCACTGATAAAATTGCTCCACACACTTGTCGATTATCCGACATGACGTATTCGGCTCCGGTGATAGTGAATGTTGAGTATGTTGAGGGGGCACATGATCTGACACCGAGACGGAAAGCTGGTGTCAAGATTGGCACAATGCCGATAATGTTAAGGAGTTGTAAGTGCACTTTAGCTGGGAAGAGCGACTCGGAGCTTGCGAAGCTCGGGGAGTGTCCGCTTGATCCGGGAGGCTATTTTGTGATTAGAGGGAGTGAGAGGGTGCTGTTGATTCAGGAGCAGCTTTCGAAGAACAGGATTATTATAGGCAAGGACAAGAAGGGGAATGTGACTGCTTCTGTGACTAGCAGCACCGAGAAAATGAAGAGCAAGACTGTGATTGAGTTTGATAAGAGTAAGAAGACGGTTTCTTTGCTGATCAATGCGTTTGAGAAAAAGGTTCCGATTATGGTGGTGATGAAGGCTATGGGGATGCAGAGTGATCAGGAAGTTGTGCAGATGCTTGGGAGAGATCCGCGGTATGGTGCATTGCTTTTGCCATCGATTGAGGAATGTACAAAGGTGGAGATATACAACAAGGAGCAGGCATTGGTGTACCTGGAAGGAAAGCTGGCGAAGAAGCGTGAGAAGGATGGTACTCATGGTAGAGTTATCTCAGTTCTTGCAGATGTGCTTCTTGCTAATGTTGCGGTGACGCGGGGAAATTTTCATCTGAAATGCATGTATGTTGGTGTCATGTTGAGACGCATGATGGATTCAGTTTTGAACAAGGGTGCAGTGGATGACACAGATTATGTTGGCAACAAGCGGTTCGAGTTATCTGGACAATTGATTTCTCTCCTGTTTGAGGATCAGTTCAAGACAATGATCGAGACAGCTAGGAAAGAATTAGACACTAAAATGTTACCAAAGGCAAAGCCTGGGAGGGGTTTGGCCTTGCTCTCTCTCATACAGAAACCGCGTTTATCAGAAGGACTGGAAAGAGCCCTGTCTACAGGAAACTTTATTATGGACCGGTTCAGAATGAAAAAGTCAGGGGTGACTCAGGTGCTAACTAGGCTATCCTTTATAGGAGCTCTGGGCTCAATGACCAAAACAAAGCCGCAAGTTGAAAAGTCAGCGAAAGTAAGTGGACCTAGAGCCTTGCAACCTAGTCAGTGGGGAATGCTTTGTCCTTGTGACACTCCTGAAGGTCAAGATATTGGACTGGTGAAGAACTTGGCCCTGCTGGCTCATGTCACAACTGATGAAGAGGATGGTCCATTGATTTCTCTGTGCTACTCTTTGGGTGTTGAAGACTTGGAACAACTATCAGCAGAGGAGCTTCACTCGCAGAATTCTTTTCTGGTTCTATTTAATGGAAACATCCTTGGAGTGCATAGAAAGCCACAGAGGTTTGGCACTGCAATGAGAAAGTTGCGTAGAGCTGGAAAAATTGGGCAGTTTGTAAGTGTCTTTGTCAATGAGAAGCAGAGTTGTGTTCAAATTGCTTCTGACGGTGGTCGAGTTTGCCGTCCGCTAATTATTGCTGACAAGGGGATCTCAAGGGTGAAAGGACATCACATGGAGGAGTTGAAGGCTGGCTACCGAACTTTTGATGATTTTTTGAATGACGGTTTGATTGAGTATCTTGATGTCAATGAGCAGAACAATGCTTTGATTGCTCTGTACGAAAATCAGACAACAGCAGAAACAACTCATATAGAAATAGAGCCTTTAGGCATTTTAGGTGTTATTGCTGGGCTTATTCCGTATCCTCATCACAACCAGTCACCTAGAAATACTTACCAATGTGCTATGGGAAAACAAGCAATGGGGAATATTGCCTATAACCAGCTGCGTCGGATGGACACGCTTCTCTACCTACTGGTGTATCCTCAAAGGCCATTATTGACTACCAAGACAATTGAGCTGGTGGGGTATGATAAACTTGGAGCTGGGCAGAATGCAACTGTGGCTGTGATGAGTTATAGCGGCTATGACATAGAGGACGCAATAGTGATGAACAAGTCATCTCTGGATCGTGGGTTTGGTCGTTCTATATATATGAAAAGATTCAGCGATGGCATCAAAACTTATAAAACTCGAGGGTCTGAAGTCGTCCACCAAGACATAATAGCCAGGCCAGATAGAGATGGAAAATATTATAATGAAAAAATGCGGGTGCTTGATGATGATGGAATTGCTTTACCTGGAGAGATTATTGCACCAGATGAAATCCTTCTGAACAAACAGGTCCCAAGTACAAACAAGCAAGGACATAGGCCTGCACCCTTGAAATACAAAGGACCTGAAGGGGAGTCTTATGTGCTGGATAAAGTCTCTCTGTATCCTGACACGTATAATAATTTGCAGTGCAAATTCTTAGTTCGCCATACTCGTAGACCGGAGGTTGGTGACAAATTCAGTAGCAGACATGGGCAGAAAGGTGTCTGTGGTACCATTGTCCAACAGGAAGATTTCCCATTTTCTGAACGTGGCATTTGCCCTGACCTAATTATGAACCCTCATGGATTTCCAAGTCGGATGACTGTAGGCAAGATGATAGAGCTTCTTGGGAGTAAAGCAGGAGTTTCATGTGGCAGATTTCACTATGGCAGTGCCTTTGGTGAACCAAGTGGTCATGCAGACAAGGTTGAAACCATAACTGAAACGCTTGTGAAGATGGGATTCAGCTATGATGGCAAGGACTTTCTTTACTCAGGACTTACAGGTTGCCCTTTACAAGCATATATTTTCATGGGACCAATTTACTACCAGAGGTTGAAACATATGGTCATAGACAAAATGCATGCACGAGGTAGTGGTCCTAAAGCTCAACTAACTAGGCAACCTACAGAGGGAAAGGCTCGTAATGGAGGACTGCGAATAGGTGAAATGGAGCGTGACTGTCTGGTTGGTTATGGTGCAAGTATGTTGGCATATGAGCGCCTAATGCTCGCTAGTGATCCTTTTGAAATTCATGTCTGCAGAGAGTGTGGTTTGCTCGGCTATTATGACCACAAGCGGAAATCCTCAATGTGCACCAACTGTAGAAATGGGAATGTTATGGCTACCATGAAGCTTCCATATGCTTGCAAGCTCTTGATCCAAGAACTCCAGTCCATGAATATTGTTCCTCGCTTGAAATTGGCAAATGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006383 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032501 GO:0032502 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045089 GO:0046483 GO:0048229 GO:0048518 GO:0048583 GO:0048584 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080134 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1145

Amino Acids

127.09

Weight (kDa)

9.12

Isoelectric Point (pI)

38.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	45 - 398	2.8e-47	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	194 - 367	5.5e-23	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	446 - 510	2.3e-16	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	547 - 607	1.1e-23	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_6	PF00562	676 - 1048	9.4e-129	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1050 - 1140	3.7e-28	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45140 AT5G45140 AT5G45140
fragaria_vesca	FvH4_5g31720 FvH4_7g03930
malus_domestica	MD14G1029000.v1.1
prunus_persica	Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1
pyrus_communis	pycom14g02710
rosa_chinensis	RchiOBHm_Chr1g0327591 RchiOBHm_Chr7g0227611
rosa_laevigata	RLG00000001632 RLG00000029981
rosa_multiflora	Rmu_co8491793.1_g000001 Rmu_sc0001772.1_g000005 Rmu_ssc0000042.1_g000027
rosa_roxburghii	Rroxscaffold_3G00232000 Rroxscaffold_4G00322550
rosa_rugosa	Rorug01G0068500 Rorug07G0242400
rosa_samantha	Rh1AG085800 Rh1BG068400 Rh1CG083300 Rh1DG090000 Rh7AG395800 Rh7BG377500 Rh7CG414500 Rh7DG391600
rosa_wichuraiana	Rw1G006690 Rw7G032920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	2370, 2439
Acc65I	GGTACC	1 cut(s)	2729
AccB1I	GGYRCC	2 cut(s)	70, 2729
AccI	GTMKAC	2 cut(s)	1283, 2674
AccII	CGCG	4 cut(s)	198, 808, 988, 1252
AclWI	GGATC	9 cut(s)	140, 221, 509, 797, 1149, 1838, 2319, 3233, 3373
AcsI	RAATTY	7 cut(s)	95, 234, 994, 1627, 2651, 2693, 3249
AcuI	CTGAAG	3 cut(s)	1500, 2406, 2604
AcyI	GRCGYC	1 cut(s)	71
AfaI	GTAC	6 cut(s)	847, 887, 928, 1961, 2536, 2731
AfiI	CCNNNNNNNGG	7 cut(s)	38, 282, 812, 1138, 1217, 2684, 3131
AflIII	ACRYGT	1 cut(s)	2628
AgeI	ACCGGT	1 cut(s)	1305
AjnI	CCWGG	6 cut(s)	63, 281, 888, 1210, 2411, 2482
Alw21I	GWGCWC	3 cut(s)	461, 1369, 3317
Alw26I	GTCTC	4 cut(s)	394, 1027, 1157, 2617
Alw44I	GTGCAC	2 cut(s)	457, 3313
AlwI	GGATC	9 cut(s)	140, 221, 509, 797, 1149, 1838, 2319, 3233, 3373
Ama87I	CYCGRG	2 cut(s)	497, 2376
AoxI	GGCC	6 cut(s)	270, 1224, 1512, 2174, 2414, 2555
ApaLI	GTGCAC	2 cut(s)	457, 3313
ApeKI	GCWGC	4 cut(s)	106, 571, 639, 2128
ApoI	RAATTY	7 cut(s)	95, 234, 994, 1627, 2651, 2693, 3249
ArsI	GACNNNNNNTTYG	2 cut(s)	685, 717
AsiGI	ACCGGT	1 cut(s)	1305
Asp700I	GAANNNNTTC	2 cut(s)	2345, 2511
Asp718I	GGTACC	1 cut(s)	2729
AspLEI	GCGC	4 cut(s)	73, 198, 200, 3222
AspS9I	GGNCC	9 cut(s)	28, 1303, 1424, 1513, 1550, 2527, 2579, 3035, 3098
AsuC2I	CCSGG	2 cut(s)	39, 519
AsuII	TTCGAA	1 cut(s)	578
AvaI	CYCGRG	2 cut(s)	497, 2376
AvaII	GGWCC	8 cut(s)	28, 1303, 1424, 1550, 2527, 2579, 3035, 3098
BaeGI	GKGCMC	3 cut(s)	385, 461, 3317
BaeI	ACNNNNGTAYC	4 cut(s)	2147, 2180, 2721, 2754
BanI	GGYRCC	2 cut(s)	70, 2729
BanII	GRGCYC	3 cut(s)	1279, 1369, 1376
BarI	GAAGNNNNNNTAC	2 cut(s)	1859, 1891
BauI	CACGAG	1 cut(s)	3087
BbsI	GAAGAC	2 cut(s)	698, 1593
Bbv12I	GWGCWC	3 cut(s)	461, 1369, 3317
BbvI	GCAGC	4 cut(s)	93, 583, 651, 2115
BceAI	ACGGC	1 cut(s)	1788
BcgI	CGANNNNNNTGC	2 cut(s)	80, 114
BciT130I	CCWGG	6 cut(s)	65, 283, 890, 1212, 2413, 2484
BciVI	GTATCC	3 cut(s)	2055, 2175, 2631
BclI	TGATCA	2 cut(s)	708, 772
BcnI	CCSGG	2 cut(s)	39, 519
BcoDI	GTCTC	4 cut(s)	394, 1027, 1157, 2617
BfaI	CTAG	8 cut(s)	636, 1170, 1347, 1428, 1443, 2067, 3117, 3234
BfmI	CTRYAG	5 cut(s)	1284, 2823, 3126, 3259, 3322
BfoI	RGCGCY	2 cut(s)	74, 3223
BfuI	GTATCC	3 cut(s)	2055, 2175, 2631
BglI	GCCNNNNNGGC	1 cut(s)	1520
BisI	GCNGC	7 cut(s)	69, 107, 572, 640, 1394, 2129, 2266
BlsI	GCNGC	7 cut(s)	70, 108, 573, 641, 1395, 2130, 2267
Bme1390I	CCNGG	8 cut(s)	39, 65, 283, 519, 890, 1212, 2413, 2484
Bme18I	GGWCC	8 cut(s)	28, 1303, 1424, 1550, 2527, 2579, 3035, 3098
BmeT110I	CYCGRG	2 cut(s)	497, 2376
BmgT120I	GGNCC	9 cut(s)	28, 1303, 1424, 1513, 1550, 2527, 2579, 3035, 3098
BmiI	GGNNCC	8 cut(s)	30, 43, 72, 348, 732, 2529, 2731, 3036
BmrFI	CCNGG	8 cut(s)	39, 65, 283, 519, 890, 1212, 2413, 2484
BmsI	GCATC	3 cut(s)	753, 780, 2367
BoxI	GACNNNNGTC	1 cut(s)	1791
BpiI	GAAGAC	2 cut(s)	698, 1593
BplI	GAGNNNNNCTC	2 cut(s)	1604, 1636
BpmI	CTGGAG	2 cut(s)	2505, 3374
Bpu14I	TTCGAA	1 cut(s)	578
BpuEI	CTTGAG	2 cut(s)	104, 1819
BpuMI	CCSGG	2 cut(s)	39, 519
Bsa29I	ATCGAT	2 cut(s)	51, 833
BsaAI	YACGTR	1 cut(s)	2631
BsaHI	GRCGYC	1 cut(s)	71
BsaJI	CCNNGG	5 cut(s)	37, 282, 806, 1211, 1660
BsaWI	WCCGGW	4 cut(s)	176, 349, 1305, 2677
BsaXI	ACNNNNNCTCC	2 cut(s)	26, 56
Bsc4I	CCNNNNNNNGG	7 cut(s)	38, 282, 812, 1138, 1217, 2684, 3131
Bse118I	RCCGGY	2 cut(s)	10, 1305
Bse1I	ACTGG	5 cut(s)	1272, 1503, 2059, 2163, 3391
Bse3DI	GCAATG	3 cut(s)	818, 1704, 2109
BseBI	CCWGG	6 cut(s)	65, 283, 890, 1212, 2413, 2484
BseCI	ATCGAT	2 cut(s)	51, 833
BseDI	CCNNGG	5 cut(s)	37, 282, 806, 1211, 1660
BseGI	GGATG	8 cut(s)	292, 768, 928, 1078, 1552, 1656, 2142, 2823
BseLI	CCNNNNNNNGG	7 cut(s)	38, 282, 812, 1138, 1217, 2684, 3131
BseMI	GCAATG	3 cut(s)	818, 1704, 2109
BseMII	CTCAG	3 cut(s)	960, 1349, 3006
BseNI	ACTGG	5 cut(s)	1272, 1503, 2059, 2163, 3391
BsePI	GCGCGC	1 cut(s)	196
BseRI	GAGGAG	2 cut(s)	1625, 1874
BseSI	GKGCMC	3 cut(s)	385, 461, 3317
BseXI	GCAGC	4 cut(s)	93, 583, 651, 2115
BseYI	CCCAGC	3 cut(s)	467, 2030, 2228
BsgI	GTGCAG	3 cut(s)	806, 1086, 2544
Bsh1236I	CGCG	4 cut(s)	198, 808, 988, 1252
BshFI	GGCC	6 cut(s)	272, 1226, 1514, 2176, 2416, 2557
BshNI	GGYRCC	2 cut(s)	70, 2729
BshTI	ACCGGT	1 cut(s)	1305
BshVI	ATCGAT	2 cut(s)	51, 833
BsiHKAI	GWGCWC	3 cut(s)	461, 1369, 3317
BsiHKCI	CYCGRG	2 cut(s)	497, 2376
BsiSI	CCGG	8 cut(s)	11, 39, 110, 177, 350, 518, 1306, 2678
BslFI	GGGAC	2 cut(s)	2513, 3048
BslI	CCNNNNNNNGG	7 cut(s)	38, 282, 812, 1138, 1217, 2684, 3131
BsmAI	GTCTC	4 cut(s)	394, 1027, 1157, 2617
BsmBI	CGTCTC	2 cut(s)	394, 1027
BsmFI	GGGAC	2 cut(s)	2513, 3048
BsmI	GAATGC	2 cut(s)	1461, 2242
BsnI	GGCC	6 cut(s)	272, 1226, 1514, 2176, 2416, 2557
BsoBI	CYCGRG	2 cut(s)	497, 2376
Bsp119I	TTCGAA	1 cut(s)	578
Bsp1286I	GDGCHC	6 cut(s)	385, 461, 1279, 1369, 1376, 3317
Bsp1407I	TGTACA	1 cut(s)	845
BspANI	GGCC	6 cut(s)	272, 1226, 1514, 2176, 2416, 2557
BspCNI	CTCAG	3 cut(s)	959, 1348, 3005
BspDI	ATCGAT	2 cut(s)	51, 833
BspFNI	CGCG	4 cut(s)	198, 808, 988, 1252
BspLI	GGNNCC	8 cut(s)	30, 43, 72, 348, 732, 2529, 2731, 3036
BspMAI	CTGCAG	1 cut(s)	3263
BspPI	GGATC	9 cut(s)	140, 221, 509, 797, 1149, 1838, 2319, 3233, 3373
BspQI	GCTCTTC	2 cut(s)	467, 650
BspT104I	TTCGAA	1 cut(s)	578
BspT107I	GGYRCC	2 cut(s)	70, 2729
BsrDI	GCAATG	3 cut(s)	818, 1704, 2109
BsrFI	RCCGGY	2 cut(s)	10, 1305
BsrGI	TGTACA	1 cut(s)	845
BsrI	ACTGG	5 cut(s)	1272, 1503, 2059, 2163, 3391
BssAI	RCCGGY	2 cut(s)	10, 1305
BssECI	CCNNGG	5 cut(s)	37, 282, 806, 1211, 1660
BssHII	GCGCGC	1 cut(s)	196
BssNI	GRCGYC	1 cut(s)	71
BssSI	CACGAG	1 cut(s)	3087
BssT1I	CCWWGG	1 cut(s)	1660
Bst2BI	CACGAG	1 cut(s)	3087
Bst2UI	CCWGG	6 cut(s)	65, 283, 890, 1212, 2413, 2484
Bst4CI	ACNGT	8 cut(s)	667, 697, 1790, 1907, 2245, 2824, 3182, 3323
Bst6I	CTCTTC	3 cut(s)	467, 650, 1536
BstACI	GRCGYC	1 cut(s)	71
BstAUI	TGTACA	1 cut(s)	845
BstBAI	YACGTR	1 cut(s)	2631
BstBI	TTCGAA	1 cut(s)	578
BstDEI	CTNAG	4 cut(s)	946, 1335, 2656, 2992
BstDSI	CCRYGG	1 cut(s)	806
BstENI	CCTNNNNNAGG	1 cut(s)	1136
BstF5I	GGATG	8 cut(s)	292, 768, 928, 1078, 1552, 1656, 2142, 2823
BstFNI	CGCG	4 cut(s)	198, 808, 988, 1252
BstH2I	RGCGCY	2 cut(s)	74, 3223
BstHHI	GCGC	4 cut(s)	73, 198, 200, 3222
BstMAI	GTCTC	4 cut(s)	394, 1027, 1157, 2617
BstNI	CCWGG	6 cut(s)	65, 283, 890, 1212, 2413, 2484
BstNSI	RCATGY	2 cut(s)	1015, 3086
BstPAI	GACNNNNGTC	1 cut(s)	1791
BstSCI	CCNGG	8 cut(s)	37, 63, 281, 517, 888, 1210, 2411, 2482
BstSFI	CTRYAG	5 cut(s)	1284, 2823, 3126, 3259, 3322
BstSLI	GKGCMC	3 cut(s)	385, 461, 3317
BstUI	CGCG	4 cut(s)	198, 808, 988, 1252
BstV1I	GCAGC	4 cut(s)	93, 583, 651, 2115
BstV2I	GAAGAC	2 cut(s)	698, 1593
BstX2I	RGATCY	2 cut(s)	802, 1830
BstYI	RGATCY	2 cut(s)	802, 1830
Bsu15I	ATCGAT	2 cut(s)	51, 833
BsuI	GTATCC	3 cut(s)	2055, 2175, 2631
BsuRI	GGCC	6 cut(s)	272, 1226, 1514, 2176, 2416, 2557
BsuTUI	ATCGAT	2 cut(s)	51, 833
BtgI	CCRYGG	1 cut(s)	806
BtgZI	GCGATG	1 cut(s)	2367
BtsCI	GGATG	8 cut(s)	292, 768, 928, 1078, 1552, 1656, 2142, 2823
BtsI	GCAGTG	5 cut(s)	20, 1074, 1692, 2651, 2896
BtsIMutI	CAGTG	7 cut(s)	20, 294, 1074, 1454, 1692, 2651, 2896
CfoI	GCGC	4 cut(s)	73, 198, 200, 3222
Cfr10I	RCCGGY	2 cut(s)	10, 1305
Cfr13I	GGNCC	9 cut(s)	28, 1303, 1424, 1513, 1550, 2527, 2579, 3035, 3098
Cfr42I	CCGCGG	1 cut(s)	809
ClaI	ATCGAT	2 cut(s)	51, 833
CseI	GACGC	4 cut(s)	994, 1044, 2120, 2291
Csp6I	GTAC	6 cut(s)	846, 886, 927, 1960, 2535, 2730
CspAI	ACCGGT	1 cut(s)	1305
CviQI	GTAC	6 cut(s)	846, 886, 927, 1960, 2535, 2730
DdeI	CTNAG	4 cut(s)	946, 1335, 2656, 2992
DinI	GGCGCC	1 cut(s)	72
Eam1104I	CTCTTC	3 cut(s)	467, 650, 1536
EarI	CTCTTC	3 cut(s)	467, 650, 1536
EciI	GGCGGA	1 cut(s)	34
Ecl136II	GAGCTC	1 cut(s)	1367
Eco130I	CCWWGG	1 cut(s)	1660
Eco147I	AGGCCT	2 cut(s)	272, 2557
Eco24I	GRGCYC	3 cut(s)	1279, 1369, 1376
Eco47I	GGWCC	8 cut(s)	28, 1303, 1424, 1550, 2527, 2579, 3035, 3098
Eco53kI	GAGCTC	1 cut(s)	1367
Eco57I	CTGAAG	3 cut(s)	1500, 2406, 2604
Eco88I	CYCGRG	2 cut(s)	497, 2376
EcoICRI	GAGCTC	1 cut(s)	1367
EcoNI	CCTNNNNNAGG	1 cut(s)	1136
EcoO109I	RGGNCCY	2 cut(s)	2527, 2579
EcoRI	GAATTC	1 cut(s)	1627
EcoRII	CCWGG	6 cut(s)	63, 281, 888, 1210, 2411, 2482
EcoT14I	CCWWGG	1 cut(s)	1660
EcoT22I	ATGCAT	3 cut(s)	1013, 3084, 3433
EcoT38I	GRGCYC	3 cut(s)	1279, 1369, 1376
EgeI	GGCGCC	1 cut(s)	72
EheI	GGCGCC	1 cut(s)	72
ErhI	CCWWGG	1 cut(s)	1660
Esp3I	CGTCTC	2 cut(s)	394, 1027
FaqI	GGGAC	2 cut(s)	2513, 3048
FauI	CCCGC	2 cut(s)	981, 2445
FauNDI	CATATG	3 cut(s)	3063, 3214, 3361
FbaI	TGATCA	2 cut(s)	708, 772
FblI	GTMKAC	2 cut(s)	1283, 2674
Fnu4HI	GCNGC	7 cut(s)	69, 107, 572, 640, 1394, 2129, 2266
FokI	GGATG	8 cut(s)	299, 775, 935, 1085, 1559, 1643, 2149, 2830
FriOI	GRGCYC	3 cut(s)	1279, 1369, 1376
Fsp4HI	GCNGC	7 cut(s)	69, 107, 572, 640, 1394, 2129, 2266
FspBI	CTAG	8 cut(s)	636, 1170, 1347, 1428, 1443, 2067, 3117, 3234
GlaI	GCGC	4 cut(s)	72, 197, 199, 3221
GluI	GCNGC	7 cut(s)	69, 107, 572, 640, 1394, 2129, 2266
GsaI	CCCAGC	3 cut(s)	471, 2034, 2232
GsuI	CTGGAG	2 cut(s)	2505, 3374
HaeII	RGCGCY	2 cut(s)	74, 3223
HaeIII	GGCC	6 cut(s)	272, 1226, 1514, 2176, 2416, 2557
HapII	CCGG	8 cut(s)	11, 39, 110, 177, 350, 518, 1306, 2678
HgaI	GACGC	4 cut(s)	994, 1044, 2120, 2291
HhaI	GCGC	4 cut(s)	73, 198, 200, 3222
Hin1I	GRCGYC	1 cut(s)	71
Hin6I	GCGC	4 cut(s)	71, 196, 198, 3220
HinP1I	GCGC	4 cut(s)	71, 196, 198, 3220
HincII	GTYRAC	1 cut(s)	172
HindII	GTYRAC	1 cut(s)	172
HindIII	AAGCTT	1 cut(s)	3353
HpaII	CCGG	8 cut(s)	11, 39, 110, 177, 350, 518, 1306, 2678
Hpy166II	GTNNAC	9 cut(s)	172, 459, 886, 1284, 1424, 2395, 2675, 2903, 3315
Hpy8I	GTNNAC	9 cut(s)	172, 459, 886, 1284, 1424, 2395, 2675, 2903, 3315
Hpy99I	CGWCG	1 cut(s)	2136
HpyCH4III	ACNGT	8 cut(s)	667, 697, 1790, 1907, 2245, 2824, 3182, 3323
HpyCH4IV	ACGT	3 cut(s)	338, 2630, 2767
HpyF3I	CTNAG	4 cut(s)	946, 1335, 2656, 2992
HpySE526I	ACGT	3 cut(s)	338, 2630, 2767
Hsp92I	GRCGYC	1 cut(s)	71
HspAI	GCGC	4 cut(s)	71, 196, 198, 3220
KasI	GGCGCC	1 cut(s)	70
KpnI	GGTACC	1 cut(s)	2733
Ksp22I	TGATCA	2 cut(s)	708, 772
KspI	CCGCGG	1 cut(s)	809
LguI	GCTCTTC	2 cut(s)	467, 650
Lsp1109I	GCAGC	4 cut(s)	93, 583, 651, 2115
LweI	GCATC	3 cut(s)	753, 780, 2367
MaeI	CTAG	8 cut(s)	636, 1170, 1347, 1428, 1443, 2067, 3117, 3234
MaeII	ACGT	3 cut(s)	338, 2630, 2767
MfeI	CAATTG	2 cut(s)	1118, 2196
MflI	RGATCY	2 cut(s)	802, 1830
MhlI	GDGCHC	6 cut(s)	385, 461, 1279, 1369, 1376, 3317
Mly113I	GGCGCC	1 cut(s)	71
MlyI	GAGTC	4 cut(s)	146, 473, 1327, 2600
MmeI	TCCRAC	4 cut(s)	354, 2114, 2764, 2795
Mph1103I	ATGCAT	3 cut(s)	1013, 3084, 3433
MroXI	GAANNNNTTC	2 cut(s)	2345, 2511
MseI	TTAA	3 cut(s)	251, 443, 1647
MslI	CAYNNNNRTG	2 cut(s)	1190, 1697
MspA1I	CMGCKG	2 cut(s)	808, 2128
MspI	CCGG	8 cut(s)	11, 39, 110, 177, 350, 518, 1306, 2678
MspR9I	CCNGG	8 cut(s)	39, 65, 283, 519, 890, 1212, 2413, 2484
MunI	CAATTG	2 cut(s)	1118, 2196
Mva1269I	GAATGC	2 cut(s)	1461, 2242
MvaI	CCWGG	6 cut(s)	65, 283, 890, 1212, 2413, 2484
MvnI	CGCG	4 cut(s)	198, 808, 988, 1252
NarI	GGCGCC	1 cut(s)	71
NciI	CCSGG	2 cut(s)	39, 519
NdeI	CATATG	3 cut(s)	3063, 3214, 3361
NlaIV	GGNNCC	8 cut(s)	30, 43, 72, 348, 732, 2529, 2731, 3036
NmeAIII	GCCGAG	1 cut(s)	3258
NsiI	ATGCAT	3 cut(s)	1013, 3084, 3433
NspI	RCATGY	2 cut(s)	1015, 3086
NspV	TTCGAA	1 cut(s)	578
PaeI	GCATGC	1 cut(s)	3086
PaeR7I	CTCGAG	1 cut(s)	2376
PauI	GCGCGC	1 cut(s)	196
PceI	AGGCCT	2 cut(s)	272, 2557
PciSI	GCTCTTC	2 cut(s)	467, 650
PcsI	WCGNNNNNNNCGW	3 cut(s)	488, 1801, 2668
PctI	GAATGC	2 cut(s)	1461, 2242
PdmI	GAANNNNTTC	2 cut(s)	2345, 2511
PfeI	GAWTC	6 cut(s)	180, 189, 562, 1046, 2346, 2961
PfoI	TCCNGGA	1 cut(s)	517
PinAI	ACCGGT	1 cut(s)	1305
PkrI	GCNGC	7 cut(s)	70, 108, 573, 641, 1395, 2130, 2267
PleI	GAGTC	4 cut(s)	146, 473, 1327, 2599
PluTI	GGCGCC	1 cut(s)	74
PpsI	GAGTC	4 cut(s)	146, 473, 1327, 2599
Ppu21I	YACGTR	1 cut(s)	2631
PpuMI	RGGWCCY	2 cut(s)	2527, 2579
PshAI	GACNNNNGTC	1 cut(s)	1791
PsiI	TTATAA	2 cut(s)	2370, 2439
Psp124BI	GAGCTC	1 cut(s)	1369
Psp5II	RGGWCCY	2 cut(s)	2527, 2579
Psp6I	CCWGG	6 cut(s)	63, 281, 888, 1210, 2411, 2482
PspFI	CCCAGC	3 cut(s)	467, 2030, 2228
PspGI	CCWGG	6 cut(s)	63, 281, 888, 1210, 2411, 2482
PspN4I	GGNNCC	8 cut(s)	30, 43, 72, 348, 732, 2529, 2731, 3036
PspPI	GGNCC	9 cut(s)	28, 1303, 1424, 1513, 1550, 2527, 2579, 3035, 3098
PspPPI	RGGWCCY	2 cut(s)	2527, 2579
PspXI	VCTCGAGB	1 cut(s)	2376
PstI	CTGCAG	1 cut(s)	3263
PsuI	RGATCY	2 cut(s)	802, 1830
PteI	GCGCGC	1 cut(s)	196
PvuII	CAGCTG	1 cut(s)	2128
RsaI	GTAC	6 cut(s)	847, 887, 928, 1961, 2536, 2731
RsaNI	GTAC	6 cut(s)	846, 886, 927, 1960, 2535, 2730
RseI	CAYNNNNRTG	2 cut(s)	1190, 1697
SacI	GAGCTC	1 cut(s)	1369
SacII	CCGCGG	1 cut(s)	809
SapI	GCTCTTC	2 cut(s)	467, 650
SaqAI	TTAA	3 cut(s)	251, 443, 1647
SatI	GCNGC	7 cut(s)	69, 107, 572, 640, 1394, 2129, 2266
Sau96I	GGNCC	9 cut(s)	28, 1303, 1424, 1513, 1550, 2527, 2579, 3035, 3098
SchI	GAGTC	4 cut(s)	146, 473, 1327, 2600
ScrFI	CCNGG	8 cut(s)	39, 65, 283, 519, 890, 1212, 2413, 2484
SduI	GDGCHC	6 cut(s)	385, 461, 1279, 1369, 1376, 3317
SfaNI	GCATC	3 cut(s)	753, 780, 2367
SfcI	CTRYAG	5 cut(s)	1284, 2823, 3126, 3259, 3322
SfoI	GGCGCC	1 cut(s)	72
Sfr274I	CTCGAG	1 cut(s)	2376
Sfr303I	CCGCGG	1 cut(s)	809
SfuI	TTCGAA	1 cut(s)	578
SgrBI	CCGCGG	1 cut(s)	809
SinI	GGWCC	8 cut(s)	28, 1303, 1424, 1550, 2527, 2579, 3035, 3098
SlaI	CTCGAG	1 cut(s)	2376
SmiMI	CAYNNNNRTG	2 cut(s)	1190, 1697
SmlI	CTYRAG	3 cut(s)	119, 1834, 2376
SmoI	CTYRAG	3 cut(s)	119, 1834, 2376
SphI	GCATGC	1 cut(s)	3086
SseBI	AGGCCT	2 cut(s)	272, 2557
SspDI	GGCGCC	1 cut(s)	70
SspI	AATATT	3 cut(s)	2113, 2435, 3403
SspMI	CTAG	8 cut(s)	636, 1170, 1347, 1428, 1443, 2067, 3117, 3234
SstI	GAGCTC	1 cut(s)	1369
StuI	AGGCCT	2 cut(s)	272, 2557
StyD4I	CCNGG	8 cut(s)	37, 63, 281, 517, 888, 1210, 2411, 2482
StyI	CCWWGG	1 cut(s)	1660
TaaI	ACNGT	8 cut(s)	667, 697, 1790, 1907, 2245, 2824, 3182, 3323
TaiI	ACGT	3 cut(s)	341, 2633, 2770
TaqI	TCGA	8 cut(s)	51, 322, 578, 833, 1104, 1161, 1795, 2377
TatI	WGTACW	2 cut(s)	845, 2534
TauI	GCSGC	3 cut(s)	71, 1396, 2268
TfiI	GAWTC	6 cut(s)	180, 189, 562, 1046, 2346, 2961
Tru1I	TTAA	3 cut(s)	251, 443, 1647
Tru9I	TTAA	3 cut(s)	251, 443, 1647
TscAI	CASTG	7 cut(s)	27, 301, 1074, 1454, 1699, 2651, 2896
TseI	GCWGC	4 cut(s)	106, 571, 639, 2128
TspGWI	ACGGA	3 cut(s)	265, 418, 2031
TspRI	CASTG	7 cut(s)	27, 301, 1074, 1454, 1699, 2651, 2896
VneI	GTGCAC	2 cut(s)	457, 3313
VpaK11BI	GGWCC	8 cut(s)	28, 1303, 1424, 1550, 2527, 2579, 3035, 3098
XagI	CCTNNNNNAGG	1 cut(s)	1136
XapI	RAATTY	7 cut(s)	95, 234, 994, 1627, 2651, 2693, 3249
XceI	RCATGY	2 cut(s)	1015, 3086
XhoI	CTCGAG	1 cut(s)	2376
XmiI	GTMKAC	2 cut(s)	1283, 2674
XmnI	GAANNNNTTC	2 cut(s)	2345, 2511
XspI	CTAG	8 cut(s)	636, 1170, 1347, 1428, 1443, 2067, 3117, 3234
Zsp2I	ATGCAT	3 cut(s)	1013, 3084, 3433

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.