Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0003584

Physical Location & Seq

Genomic Coordinates Forward (+)

52634266 .. 52655166

Gene Structure 20901 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000029981

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2709 bp

ATGGTGGTCATGAAGGCTATGGGAATGACTAGTGATCAAGAAGTTGTACAGATGGTTGGAAGAGATCCTCGATATGGTGCCCTGCTCATGCCATCTATTGAGGAATGTGCAAAGGAAGGTATATATACAAAAGAGCAAGCACTGCTGTATCTCGAATCAAAGGTGAAGAGGTCCATGTTTGATCGTCCTTCATCCGATAATGACTGTAAAGTAATGGCAGTTCTTCGAGATATATTTATTGCTAATGTTCCGGTATGCCGGTCCAATTTTCGTCCAAAATGCATATATGTTGCTGTCATGTTGAGGCGCATAATGGATGCAATTTTAAACAAGGATGCGATGGATGACAAGGATTATGTTGGGAACAAGCGGCTTGAGCTATCTGGGCAATCTATTTCTCTTCTGTTTGAGGATCTGTTCAAGACAATGATCTCTGAAGTTAGGAAGACTGTAGACAATATATTAACAAAGCCTAGCAGGTCTAGTCGTTTTGACTTGGCTCAGTACATACGCAAAGATAGTATTTCACATGGCCTTGAAAGAACCCTTTCTACTGGAAACTTTGATCTCAAACGGTTCAGGATGCACAGGACAGGCATGACACAGGTGCTAGCTAGGTTATCTTTCATTGGGTCTCTAGGTCAAATGACCAGAACAAAACCGCAGTTTGAGAAGTCACGAAAAGTGAGTGGACCTAGAGCCTTGCAACCTAGCCAGTGGGGCATGCTTTGCCCTTGTGACACTCCAGAAGGTGAAGCATGTGGACTGGTAAAAAACTTAGCCCTGATGGCCCATGTAACAACTGATGAAGAGGAGGGCCCTTTAATATCTCTGTGCAAATGCTTGGGTGTGGAAGATTTGGAAACACTATCAGCAGAGGAGCTTCACTCACCCAATTCTTTTCTGGTTTTATTCAATGGAGACATCCTTGGAAAGCACAGGAAGCCACAGCGTTTTGCCGCTGCAATGAGAAAGTTGCGTAGAGCTGGGAAGATTGGAGAATTTGTTAGTGTCTTTGTCAATGAGAAGCAGCATTGTGTTTACATTGCTTCTGATGGTGGCCGAGTTTGTCGGCCACTAGTTATCGCTGACAAGGGCATTTCAAGAGTTAAAGAATTTCACATGCAGGAGCTGAATGCTGGGGTCCGTACTTTTAATGACTTTTTAAGTGACGGGTTGATTGAGTATCTTGATGTTAATGAGCAGAATAATGCTTTGATTGCTCTATACGAAGATGAGGCAACAACAGAAACAACTCATATAGAAATAGAGCCTTTCACCATCTTAGGTGTTATTGCTGGGCTAATTCCGTATCCTCACCACAATCAGTCGCCTAGAAATACTTACCAGTGTGCAATGGGGAAGCAAGCAATGGGGAATATCGCATATAACCAGCTTCGTCGGATAGACTTGCTTTACCTACTGGTGTATCCCCAACGACCATTATTGACAACAAAGACCATTGAGCTGGTGGGCTTTGATAAACTCGGAGCTGGGCAGAATGCAACTGTTGCTGTGATGAGTTATAGTGGCTATGACATAGAGGATGCAATCGTCATGAACAAGGCATCTCTGGATCGTGGTTTTGGTCGTTGTATTCTTATGAAAAAGTTTGTTGTCGCCAATCAAAATTATAGCAATAACACACAAGACAGAATAATCAGGCCAAGTAAAGATGAAGTTGATTCTGGAAAGATGCAGGTTCTCGATGATGATGGTATTGCTGCACCTGGGGAAATTATTAGATCAAATGACATCTATGTAAAGAAGCAGGTCCCTATTGTCACAAGGGGGGCTATTAGATCGGGATTAAAGGATAGTGAGTATCGGTCTGCCTTTCAAAAATATAAAGGACCTGATGGGGAGTCTTGTGTGGTGGATAAAGTAGCTCTCTTTTCTGATACACATAATAATTTATGTGTTAAATTATTGATTCGCCATACTCGTAGACCAGAGGTTGGTGACAAGTTTAGTAGCAGACATGGGCAGAAAGGTGTTTGTGGTACAATTGTCCAGCAGGAAGATTTCCCATTTTCTGAACGTGGCATTTGCCCTGATTTAATCATGAACCCTCATGGATTTCCAAGCCGAATGACTGTTGGCAAGATGATTGAGCTTCTTGGGGGTAAAGCAGGAGTTTCATGTGGTAGGTTTCATTATGGTAGTGCCTTTGGTGAACCAAGTGGTCATGCAGACAAGGTTGAGGCTATAAGTGAAACACTCGTGAGGATGGGATTTAGCTATGATGGCAAAGACTTCTTGTGCTCAGGAATCACTGGTTGCCCATTGCAAGCATATATTTTCATGGGACCAATTTACTACCAGAAGTTAAAACACATGGTCCTTGATAAAATGCATGCACGTGGTAGTGGTCCCAGAGTCAGTCTAACTAGACAGCCTACTGAGGGAAAAGCTCGTAATGGAGGACTACGAGTAGGAGAAATGGAGCGTGATTGTCTGATTGCTTATGGTGCCAGTATGTTGCTGTATGAGCGCCTAATGATCTCTAGTGATCCTTTCGAAGTTCAGGTTTGCAGAGTGTGTGGTTTATTGGGTTATTACAACCACAAGATGAAAGCTGGAATTTGTTCCTCATGCAAAAATGGGGATAATATTTCTACCATGAAGCTTCCCTATGCTTGCAAGCTCTTGATCCAGGAACTCCAGTCGATGAACATTGTTCCCCGCTTAAAACTAGCTGAAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006383 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032501 GO:0032502 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045089 GO:0046483 GO:0048229 GO:0048518 GO:0048583 GO:0048584 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080134 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

903

Amino Acids

100.71

Weight (kDa)

8.75

Isoelectric Point (pI)

44.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_2	PF04561	1 - 126	8.7e-15	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	202 - 266	4.4e-19	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	303 - 363	2.9e-23	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_6	PF00562	432 - 806	2.6e-134	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	808 - 898	5.3e-31	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45140 AT5G45140 AT5G45140
fragaria_vesca	FvH4_5g31720 FvH4_7g03930
malus_domestica	MD14G1029000.v1.1
prunus_persica	Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1
pyrus_communis	pycom14g02710
rosa_chinensis	RchiOBHm_Chr1g0327591 RchiOBHm_Chr7g0227611
rosa_laevigata	RLG00000001632 RLG00000029981
rosa_multiflora	Rmu_co8491793.1_g000001 Rmu_sc0001772.1_g000005 Rmu_ssc0000042.1_g000027
rosa_roxburghii	Rroxscaffold_3G00232000 Rroxscaffold_4G00322550
rosa_rugosa	Rorug01G0068500 Rorug07G0242400
rosa_samantha	Rh1AG085800 Rh1BG068400 Rh1CG083300 Rh1DG090000 Rh7AG395800 Rh7BG377500 Rh7CG414500 Rh7DG391600
rosa_wichuraiana	Rw1G006690 Rw7G032920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	468, 1690, 1762
AccB1I	GGYRCC	2 cut(s)	77, 2471
AccB7I	CCANNNNNTGG	1 cut(s)	1958
AccI	GTMKAC	2 cut(s)	453, 1948
AciI	CCGC	4 cut(s)	370, 662, 960, 2686
AclWI	GGATC	5 cut(s)	59, 420, 1584, 2507, 2647
AcoI	YGGCCR	2 cut(s)	1060, 1073
AcsI	RAATTY	3 cut(s)	1001, 1115, 2583
AcuI	CTGAAG	1 cut(s)	456
AcvI	CACGTG	1 cut(s)	2363
AfaI	GTAC	4 cut(s)	48, 506, 1150, 2005
AfiI	CCNNNNNNNGG	3 cut(s)	74, 1958, 2405
AgsI	TTSAA	5 cut(s)	421, 539, 916, 1104, 1841
AhlI	ACTAGT	2 cut(s)	29, 1078
AjnI	CCWGG	2 cut(s)	1729, 2655
Alw21I	GWGCWC	1 cut(s)	2267
Alw26I	GTCTC	2 cut(s)	639, 915
AlwI	GGATC	5 cut(s)	59, 420, 1584, 2507, 2647
AlwNI	CAGNNNCTG	1 cut(s)	1132
AoxI	GGCC	6 cut(s)	532, 789, 817, 1060, 1073, 1664
ApaI	GGGCCC	1 cut(s)	821
ApeKI	GCWGC	3 cut(s)	962, 1030, 1724
ApoI	RAATTY	3 cut(s)	1001, 1115, 2583
Asp700I	GAANNNNTTC	2 cut(s)	547, 2587
AspLEI	GCGC	2 cut(s)	309, 2496
AsuHPI	GGTGA	7 cut(s)	175, 764, 882, 1270, 1310, 1973, 2186
AsuII	TTCGAA	1 cut(s)	2520
AsuNHI	GCTAGC	1 cut(s)	610
AvaII	GGWCC	9 cut(s)	171, 261, 692, 1144, 1774, 1853, 2309, 2341, 2372
BaeGI	GKGCMC	2 cut(s)	82, 821
BaeI	ACNNNNGTAYC	6 cut(s)	131, 164, 1412, 1445, 1995, 2028
BanI	GGYRCC	2 cut(s)	77, 2471
BanII	GRGCYC	1 cut(s)	821
BauI	CACGAG	1 cut(s)	2222
BbrPI	CACGTG	1 cut(s)	2363
BbsI	GAAGAC	1 cut(s)	452
Bbv12I	GWGCWC	1 cut(s)	2267
BbvI	GCAGC	3 cut(s)	949, 1042, 1711
BciT130I	CCWGG	2 cut(s)	1731, 2657
BciVI	GTATCC	2 cut(s)	1323, 1440
BclI	TGATCA	1 cut(s)	34
BcoDI	GTCTC	2 cut(s)	639, 915
BcuI	ACTAGT	2 cut(s)	29, 1078
BfmI	CTRYAG	1 cut(s)	450
BfoI	RGCGCY	1 cut(s)	2497
BfuAI	ACCTGC	3 cut(s)	468, 1690, 1762
BfuI	GTATCC	2 cut(s)	1323, 1440
BglI	GCCNNNNNGGC	2 cut(s)	720, 788
BisI	GCNGC	5 cut(s)	371, 960, 963, 1031, 1725
BlsI	GCNGC	5 cut(s)	372, 961, 964, 1032, 1726
Bme1390I	CCNGG	2 cut(s)	1731, 2657
Bme18I	GGWCC	9 cut(s)	171, 261, 692, 1144, 1774, 1853, 2309, 2341, 2372
BmiI	GGNNCC	7 cut(s)	79, 819, 1145, 1776, 2310, 2374, 2473
BmrFI	CCNGG	2 cut(s)	1731, 2657
BmsI	GCATC	6 cut(s)	307, 325, 573, 1537, 1577, 1686
BmtI	GCTAGC	1 cut(s)	614
BpiI	GAAGAC	1 cut(s)	452
BplI	GAGNNNNNCTC	2 cut(s)	872, 904
BpmI	CTGGAG	2 cut(s)	729, 2648
Bpu10I	CCTNAGC	1 cut(s)	2266
Bpu14I	TTCGAA	1 cut(s)	2520
BpuEI	CTTGAG	1 cut(s)	395
BsaAI	YACGTR	1 cut(s)	2363
BsaI	GGTCTC	1 cut(s)	639
BsaJI	CCNNGG	2 cut(s)	928, 1730
BsaWI	WCCGGW	1 cut(s)	250
BsaXI	ACNNNNNCTCC	2 cut(s)	990, 1020
Bsc4I	CCNNNNNNNGG	3 cut(s)	74, 1958, 2405
Bse118I	RCCGGY	1 cut(s)	258
Bse1I	ACTGG	8 cut(s)	559, 715, 771, 1348, 1428, 2281, 2475, 2665
Bse3DI	GCAATG	5 cut(s)	972, 1044, 1362, 1377, 2285
BseBI	CCWGG	2 cut(s)	1731, 2657
BseDI	CCNNGG	2 cut(s)	928, 1730
BseGI	GGATG	8 cut(s)	191, 322, 340, 349, 588, 924, 1552, 2235
BseLI	CCNNNNNNNGG	3 cut(s)	74, 1958, 2405
BseMI	GCAATG	5 cut(s)	972, 1044, 1362, 1377, 2285
BseMII	CTCAG	3 cut(s)	515, 2280, 2394
BseNI	ACTGG	8 cut(s)	559, 715, 771, 1348, 1428, 2281, 2475, 2665
BseRI	GAGGAG	2 cut(s)	827, 893
BseSI	GKGCMC	2 cut(s)	82, 821
BseXI	GCAGC	3 cut(s)	949, 1042, 1711
BseYI	CCCAGC	4 cut(s)	986, 1139, 1298, 1493
BsgI	GTGCAG	1 cut(s)	1710
BshFI	GGCC	6 cut(s)	534, 791, 819, 1062, 1075, 1666
BshNI	GGYRCC	2 cut(s)	77, 2471
BsiHKAI	GWGCWC	1 cut(s)	2267
BsiSI	CCGG	2 cut(s)	251, 259
BslFI	GGGAC	3 cut(s)	1760, 2322, 2358
BslI	CCNNNNNNNGG	3 cut(s)	74, 1958, 2405
BsmAI	GTCTC	2 cut(s)	639, 915
BsmFI	GGGAC	3 cut(s)	1760, 2322, 2358
BsmI	GAATGC	2 cut(s)	1141, 1507
BsnI	GGCC	6 cut(s)	534, 791, 819, 1062, 1075, 1666
Bso31I	GGTCTC	1 cut(s)	639
Bsp119I	TTCGAA	1 cut(s)	2520
Bsp120I	GGGCCC	1 cut(s)	817
Bsp1286I	GDGCHC	3 cut(s)	82, 821, 2267
Bsp1407I	TGTACA	1 cut(s)	46
BspACI	CCGC	4 cut(s)	370, 662, 960, 2686
BspANI	GGCC	6 cut(s)	534, 791, 819, 1062, 1075, 1666
BspCNI	CTCAG	3 cut(s)	514, 2279, 2395
BspHI	TCATGA	3 cut(s)	9, 1557, 2064
BspLI	GGNNCC	7 cut(s)	79, 819, 1145, 1776, 2310, 2374, 2473
BspMI	ACCTGC	3 cut(s)	468, 1690, 1762
BspOI	GCTAGC	1 cut(s)	614
BspPI	GGATC	5 cut(s)	59, 420, 1584, 2507, 2647
BspT104I	TTCGAA	1 cut(s)	2520
BspT107I	GGYRCC	2 cut(s)	77, 2471
BspTNI	GGTCTC	1 cut(s)	639
BsrDI	GCAATG	5 cut(s)	972, 1044, 1362, 1377, 2285
BsrFI	RCCGGY	1 cut(s)	258
BsrGI	TGTACA	1 cut(s)	46
BsrI	ACTGG	8 cut(s)	559, 715, 771, 1348, 1428, 2281, 2475, 2665
BssAI	RCCGGY	1 cut(s)	258
BssECI	CCNNGG	2 cut(s)	928, 1730
BssSI	CACGAG	1 cut(s)	2222
BssT1I	CCWWGG	1 cut(s)	928
Bst2BI	CACGAG	1 cut(s)	2222
Bst2UI	CCWGG	2 cut(s)	1731, 2657
Bst4CI	ACNGT	5 cut(s)	206, 451, 576, 1510, 2098
Bst6I	CTCTTC	4 cut(s)	55, 161, 405, 804
BstAPI	GCANNNNNTGC	3 cut(s)	142, 729, 1511
BstAUI	TGTACA	1 cut(s)	46
BstBAI	YACGTR	1 cut(s)	2363
BstBI	TTCGAA	1 cut(s)	2520
BstC8I	GCNNGC	8 cut(s)	138, 612, 725, 1368, 2292, 2358, 2641, 2645
BstDEI	CTNAG	5 cut(s)	501, 778, 1285, 2266, 2403
BstF5I	GGATG	8 cut(s)	191, 322, 340, 349, 588, 924, 1552, 2235
BstH2I	RGCGCY	1 cut(s)	2497
BstHHI	GCGC	2 cut(s)	309, 2496
BstMAI	GTCTC	2 cut(s)	639, 915
BstNI	CCWGG	2 cut(s)	1731, 2657
BstNSI	RCATGY	4 cut(s)	727, 762, 1126, 2360
BstSCI	CCNGG	2 cut(s)	1729, 2655
BstSFI	CTRYAG	1 cut(s)	450
BstSLI	GKGCMC	2 cut(s)	82, 821
BstV1I	GCAGC	3 cut(s)	949, 1042, 1711
BstV2I	GAAGAC	1 cut(s)	452
BstX2I	RGATCY	2 cut(s)	64, 412
BstXI	CCANNNNNNTGG	1 cut(s)	1468
BstYI	RGATCY	2 cut(s)	64, 412
BsuI	GTATCC	2 cut(s)	1323, 1440
BsuRI	GGCC	6 cut(s)	534, 791, 819, 1062, 1075, 1666
BtgZI	GCGATG	1 cut(s)	353
BtsCI	GGATG	8 cut(s)	191, 322, 340, 349, 588, 924, 1552, 2235
BtsI	GCAGTG	1 cut(s)	140
BtsIMutI	CAGTG	4 cut(s)	140, 722, 1355, 2274
BveI	ACCTGC	3 cut(s)	468, 1690, 1762
Cac8I	GCNNGC	8 cut(s)	138, 612, 725, 1368, 2292, 2358, 2641, 2645
CaiI	CAGNNNCTG	1 cut(s)	1132
CciI	TCATGA	3 cut(s)	9, 1557, 2064
CfoI	GCGC	2 cut(s)	309, 2496
Cfr10I	RCCGGY	1 cut(s)	258
Csp6I	GTAC	4 cut(s)	47, 505, 1149, 2004
CviQI	GTAC	4 cut(s)	47, 505, 1149, 2004
DdeI	CTNAG	5 cut(s)	501, 778, 1285, 2266, 2403
DraI	TTTAAA	1 cut(s)	327
EaeI	YGGCCR	2 cut(s)	1060, 1073
Eam1104I	CTCTTC	4 cut(s)	55, 161, 405, 804
EarI	CTCTTC	4 cut(s)	55, 161, 405, 804
Eco130I	CCWWGG	1 cut(s)	928
Eco24I	GRGCYC	1 cut(s)	821
Eco31I	GGTCTC	1 cut(s)	639
Eco47I	GGWCC	9 cut(s)	171, 261, 692, 1144, 1774, 1853, 2309, 2341, 2372
Eco57I	CTGAAG	1 cut(s)	456
Eco72I	CACGTG	1 cut(s)	2363
EcoO109I	RGGNCCY	4 cut(s)	817, 818, 1774, 1853
EcoRII	CCWGG	2 cut(s)	1729, 2655
EcoT14I	CCWWGG	1 cut(s)	928
EcoT22I	ATGCAT	2 cut(s)	284, 2358
EcoT38I	GRGCYC	1 cut(s)	821
ErhI	CCWWGG	1 cut(s)	928
FaqI	GGGAC	3 cut(s)	1760, 2322, 2358
FauI	CCCGC	1 cut(s)	2693
FbaI	TGATCA	1 cut(s)	34
FblI	GTMKAC	2 cut(s)	453, 1948
Fnu4HI	GCNGC	5 cut(s)	371, 960, 963, 1031, 1725
FokI	GGATG	8 cut(s)	178, 329, 347, 356, 595, 911, 1559, 2242
FriOI	GRGCYC	1 cut(s)	821
Fsp4HI	GCNGC	5 cut(s)	371, 960, 963, 1031, 1725
GlaI	GCGC	2 cut(s)	308, 2495
GluI	GCNGC	5 cut(s)	371, 960, 963, 1031, 1725
GsaI	CCCAGC	4 cut(s)	990, 1143, 1302, 1497
GsuI	CTGGAG	2 cut(s)	729, 2648
HaeII	RGCGCY	1 cut(s)	2497
HaeIII	GGCC	6 cut(s)	534, 791, 819, 1062, 1075, 1666
HapII	CCGG	2 cut(s)	251, 259
HhaI	GCGC	2 cut(s)	309, 2496
Hin6I	GCGC	2 cut(s)	307, 2494
HinP1I	GCGC	2 cut(s)	307, 2494
HindIII	AAGCTT	2 cut(s)	2627, 2703
HinfI	GANTC	6 cut(s)	155, 1685, 1865, 1933, 2271, 2379
HpaII	CCGG	2 cut(s)	251, 259
HphI	GGTGA	7 cut(s)	175, 764, 882, 1270, 1310, 1973, 2186
Hpy166II	GTNNAC	6 cut(s)	454, 692, 764, 1042, 1949, 2177
Hpy188I	TCNGA	8 cut(s)	196, 436, 1054, 1404, 1490, 1900, 2038, 2460
Hpy8I	GTNNAC	6 cut(s)	454, 692, 764, 1042, 1949, 2177
Hpy99I	CGWCG	1 cut(s)	1404
HpyAV	CCTTC	4 cut(s)	7, 110, 198, 743
HpyCH4III	ACNGT	5 cut(s)	206, 451, 576, 1510, 2098
HpyCH4IV	ACGT	2 cut(s)	2041, 2362
HpyF3I	CTNAG	5 cut(s)	501, 778, 1285, 2266, 2403
HpySE526I	ACGT	2 cut(s)	2041, 2362
HspAI	GCGC	2 cut(s)	307, 2494
Ksp22I	TGATCA	1 cut(s)	34
LmnI	GCTCC	4 cut(s)	880, 1129, 1490, 2446
Lsp1109I	GCAGC	3 cut(s)	949, 1042, 1711
LweI	GCATC	6 cut(s)	307, 325, 573, 1537, 1577, 1686
MaeII	ACGT	2 cut(s)	2041, 2362
MaeIII	GTNAC	6 cut(s)	675, 737, 796, 1169, 1783, 1961
MfeI	CAATTG	1 cut(s)	2007
MflI	RGATCY	2 cut(s)	64, 412
MhlI	GDGCHC	3 cut(s)	82, 821, 2267
MlyI	GAGTC	2 cut(s)	1874, 2388
MmeI	TCCRAC	2 cut(s)	37, 1382
Mph1103I	ATGCAT	2 cut(s)	284, 2358
MroXI	GAANNNNTTC	2 cut(s)	547, 2587
MslI	CAYNNNNRTG	1 cut(s)	2361
MspA1I	CMGCKG	1 cut(s)	962
MspI	CCGG	2 cut(s)	251, 259
MspR9I	CCNGG	2 cut(s)	1731, 2657
MunI	CAATTG	1 cut(s)	2007
Mva1269I	GAATGC	2 cut(s)	1141, 1507
MvaI	CCWGG	2 cut(s)	1731, 2657
NheI	GCTAGC	1 cut(s)	610
NlaIV	GGNNCC	7 cut(s)	79, 819, 1145, 1776, 2310, 2374, 2473
NmeAIII	GCCGAG	1 cut(s)	1088
NmuCI	GTSAC	5 cut(s)	675, 737, 1169, 1783, 1961
NsiI	ATGCAT	2 cut(s)	284, 2358
NspI	RCATGY	4 cut(s)	727, 762, 1126, 2360
NspV	TTCGAA	1 cut(s)	2520
PaeI	GCATGC	2 cut(s)	727, 2360
PagI	TCATGA	3 cut(s)	9, 1557, 2064
PcsI	WCGNNNNNNNCGW	1 cut(s)	1942
PctI	GAATGC	2 cut(s)	1141, 1507
PdmI	GAANNNNTTC	2 cut(s)	547, 2587
PfeI	GAWTC	4 cut(s)	155, 1685, 1933, 2271
PflMI	CCANNNNNTGG	1 cut(s)	1958
PfoI	TCCNGGA	1 cut(s)	2655
PkrI	GCNGC	5 cut(s)	372, 961, 964, 1032, 1726
PleI	GAGTC	2 cut(s)	1873, 2387
PmaCI	CACGTG	1 cut(s)	2363
PmlI	CACGTG	1 cut(s)	2363
PpsI	GAGTC	2 cut(s)	1873, 2387
Ppu21I	YACGTR	1 cut(s)	2363
PpuMI	RGGWCCY	2 cut(s)	1774, 1853
Psp5II	RGGWCCY	2 cut(s)	1774, 1853
Psp6I	CCWGG	2 cut(s)	1729, 2655
PspCI	CACGTG	1 cut(s)	2363
PspFI	CCCAGC	4 cut(s)	986, 1139, 1298, 1493
PspGI	CCWGG	2 cut(s)	1729, 2655
PspN4I	GGNNCC	7 cut(s)	79, 819, 1145, 1776, 2310, 2374, 2473
PspOMI	GGGCCC	1 cut(s)	817
PspPPI	RGGWCCY	2 cut(s)	1774, 1853
PsrI	GAACNNNNNNTAC	4 cut(s)	204, 236, 535, 567
PstNI	CAGNNNCTG	1 cut(s)	1132
PsuI	RGATCY	2 cut(s)	64, 412
RsaI	GTAC	4 cut(s)	48, 506, 1150, 2005
RsaNI	GTAC	4 cut(s)	47, 505, 1149, 2004
RseI	CAYNNNNRTG	1 cut(s)	2361
SatI	GCNGC	5 cut(s)	371, 960, 963, 1031, 1725
SchI	GAGTC	2 cut(s)	1874, 2388
ScrFI	CCNGG	2 cut(s)	1731, 2657
SduI	GDGCHC	3 cut(s)	82, 821, 2267
SfaNI	GCATC	6 cut(s)	307, 325, 573, 1537, 1577, 1686
SfcI	CTRYAG	1 cut(s)	450
SfuI	TTCGAA	1 cut(s)	2520
SinI	GGWCC	9 cut(s)	171, 261, 692, 1144, 1774, 1853, 2309, 2341, 2372
SmiMI	CAYNNNNRTG	1 cut(s)	2361
SmlI	CTYRAG	1 cut(s)	374
SmoI	CTYRAG	1 cut(s)	374
SpeI	ACTAGT	2 cut(s)	29, 1078
SphI	GCATGC	2 cut(s)	727, 2360
SsiI	CCGC	4 cut(s)	370, 662, 960, 2686
SspI	AATATT	1 cut(s)	2614
StyD4I	CCNGG	2 cut(s)	1729, 2655
StyI	CCWWGG	1 cut(s)	928
TaaI	ACNGT	5 cut(s)	206, 451, 576, 1510, 2098
TaiI	ACGT	2 cut(s)	2044, 2365
TaqI	TCGA	6 cut(s)	70, 153, 226, 1707, 2520, 2669
TaqII	GACCGA	1 cut(s)	1818
TatI	WGTACW	2 cut(s)	46, 504
TauI	GCSGC	2 cut(s)	373, 962
TfiI	GAWTC	4 cut(s)	155, 1685, 1933, 2271
TscAI	CASTG	4 cut(s)	147, 722, 1355, 2281
TseFI	GTSAC	5 cut(s)	675, 737, 1169, 1783, 1961
TseI	GCWGC	3 cut(s)	962, 1030, 1724
Tsp45I	GTSAC	5 cut(s)	675, 737, 1169, 1783, 1961
TspGWI	ACGGA	2 cut(s)	1136, 1299
TspRI	CASTG	4 cut(s)	147, 722, 1355, 2281
Van91I	CCANNNNNTGG	1 cut(s)	1958
VpaK11BI	GGWCC	9 cut(s)	171, 261, 692, 1144, 1774, 1853, 2309, 2341, 2372
XapI	RAATTY	3 cut(s)	1001, 1115, 2583
XceI	RCATGY	4 cut(s)	727, 762, 1126, 2360
XcmI	CCANNNNNNNNNTGG	2 cut(s)	901, 1468
XmiI	GTMKAC	2 cut(s)	453, 1948
XmnI	GAANNNNTTC	2 cut(s)	547, 2587
Zsp2I	ATGCAT	2 cut(s)	284, 2358

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.