Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0003584

Physical Location & Seq

Genomic Coordinates Forward (+)

52856717 .. 52882818

Gene Structure 26102 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00322550.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3315 bp

ATGGGCGTACCGCAAGACTCCTTGGCGGACCCTCCTAAGAGCAGCAAGAACCAACGGTTTCGTAATGGCAAGCAAGTTCATCACATGGACATTGATAAGGAACTCCTCGCCGCCCCAATCAAATCCGCCAAGGACAAATTCGCACTCATTCCCGAGTTTCTAAAGGTGAGAGGGTTGGTAAAGCAGCATTTGGACTCGTTTAATTACTTTGTCAACACTGGAATCAAGAAAATTGTGCGTGCCAATGATCGCATAGTGTCCGGCGTCGATCCAAGTATTTGGCTCAGGTTTAATAATATCTATATTGATAAGCCCTCACTGACAACTGATGGTGTCACTACCGAAATCGCTCCCCATACATGTCGTTTATCTGATATGACGTATTCTGCTCCAATAAGAGTCGATGTAGAGTACGTTGAAGGAAGTCACGATCAAAAGAGGATAGTGACCAGGAAAGCTGTCGAAATTGCAAGAATGCCTATTATGTTACGGAGTTGCTGCTGTACCTTATATGGAAAAACAGAATCCGAACTTGCAATTCTTGGTGAGTGCCCACTTGATCCTGGAGGGTATTTTATCATCAAAGGGAATGAGAAGGTGCTTTTGATACAAGAACAGCTTTCAAAAAACAGGATTATCATTGACATGGATAAGAAGGGAAACGTAAATGCTTCTGTTACAAGCAGTTCAGAGACAATTAAAAGCAAGACTGTAATTCAGATGGAAAAACAGAAGATCTATTTGGATCTTAATCAATTTTCCAAAAAGATTCCCATTATGGTGGTCATGAAGGCTATGGGAATGACTAGTGATCAAGAAGTTGTACAGATGGTTGGAAGAGATCCTCGATATGGTGCCCTGCTCATGCCATCTATTGAGGAATGTGCAAAGGAAGGTGTATATACAAAAGAGCAAGCACTGCTGTATCTCGAATCAAAGGTGAAGAGGTCCATGTTTGATCGTCCTTCATCCGATAATGACTGTAAAGTAATGGCAGTTCTTCGAGATATATTTATTGCTAATGTTCCGGTATGCCGGTCCAATTTTCGTCCAAAATGCATATATGTTGCTGTCATGTTGAGGCGCATAATGGATGCAATTTTAAACAAGGATGCGATGGATGACAAGGATTATGTTGGGAACAAGCGGCTTGAGCTATCTGGGCAATCTATTTCTCTTCTGTTTGAGGATCTGTTCAAGACAATGATCTCTGAAGTTAGGAAGACTGTGGACAATATATTAACAAAGCCTAGCAGGTCTAGTCGTTTTGACTTGGCTCAGGAGCTGCTGGACTCTTATCTGATCCTTGGTTTGGTTGCAGTTTATGTATTTCCAAATGCAAATCTATGCGATCCAGCTCTTGGCCTCTGTTCCAATCTTTATCAGACAACTACTTTCCTCATAGTTGACCTTTACATACGCAAAGATAGTATTTCACATGGCCTTGAAAGAACCCTTTCTACTGGAAACTTTGATCTCAAACGGTTCAGGATGCACAGGACAGGCATGACACAGGTGCTAGCTAGGTTATCTTTCATTGGGTCTCTAGGCCAAATGACCAGAACAAAACCGCAGTTTGAGAAGTCACGAAAAGTGAGTGGACCTAGAGCCTTGCAACCTAGCCAGTGGGGCATGCTTTGCCCTTGTGACACTCCAGAAGGTGAAGCATGTGGACTGGTAAAAAACTTAGCCCTGATGGCCCATGTAACAACTGATGAAGAGGAGGGCCCTTTAATATCTCTGTGCAAATGCTTGGGTGTGGAAGATTTGGAAACACTATCGGCAGAGGAGCTTCACTCACCCAATTCTTTTCTGGTTTTATTCAATGGAGACATCCTTGGAAAGCACAGGAAGCCACAGCGTTTTGCCGCTGCAATGAGAAAGTTGCGTAGAGCTGGGAAGATTGGAGAATTTGTTAGTGTCTTTGTCAATGAGAAGCAGCATTGTGTTTACATTGCTTCTGATGGTGGTCGAGTTTGTCGGCCACTAGTTATCGCTGACAAGGGCATTTCAAGAGTCAAAGAATATCACATGCAGGAGCTGAATGCTGGGGTCCGTACTTTTAATGACTTTTTAAGTGACGGGTTGATCGAGTATCTTGATGTTAACGAGCAGAATAATGCTTTGATTGCTCTATACGAAGATGAGGCAACAACAGAAACAACTCATATAGAAATAGAGCCTTTCACCATCTTAGGTGTTATTGCTGGGCTAATTCCGTATCCTCACCACAATCAGTCGCCTAGAAATACTTACCAGGTGGGCTTTGATAAACTCGGAGCTGGGCAGAATGCAACTGTTGCTGTTATGAGTTATAGTGGCTATGACATAGAGGATGCAATCGTCATGAACAAGGCATCTCTGGATCGTGGTTTTGGTCGTTGTATTCTTATGAAAAAGTTTGTTGTCGCCAATCAAAATTATAGCAATAACACACAAGACAGAATAATCAGGCCAAGCAAAGATGAAGTTGATTCTGGAAAGATGCAGGTTCTCGATGATGATGGTATTGCTGCACCTGGGGAAATTATTAGATCAAATGACATCTATGTAAAGAAGCAGGTTGGTGACAAGTTTAGTAGCAGACATGGGCAGAAAGGTGTTTGTGGTACAATTGTCCAGCAGGAAGATTTCCCATTTTCTGAACGTGGCATTTGCCCTGATTTAATCATGAACCCTCATGGATTTCCAAGCCGAATGACTGTTGGCAAGATGATTGAGCTTCTTGGGGGTAAAGCAGGAGTTTCATGTGGTAGGTTTCATTATGGTAGTGCCTTTGGTGAACCAAGTGGTCATGCAGACAAGGTTGAGGCTATAAGTGAAACACTTGTGAGGATGGGATTTAGCTATGATGGCAAAGACTTCTTATGCTCAGGAATCACTGGTTGCCCATTGCAAGCATATATTTTCATGGGACCAATTTACTACCAGAAGTTAAAACACATGGTCCTTGATAAAATGCATGCACGTGGTAGTGGTCCCAGAGTCAGTCTAACTAGACAGCCTACTGAGGGAAAAGCTCGTAATGGAGGACTACGAGTAGGAGAAATGGAGCGTGATTGTCTGATTGCTTATGGTGCCAGTATGTTGCTGTATGAGCGCCTAATGATCTCTAGTGATCCTTTCGAAGTTCAGGTTTGCAGAGTGTGTGGTTTATTGGGTTATTACAACCACAAGATGAAAGCTGGAATTTGTTCCTCATGCAAAAATGGGGATAATATTTCTACCATGAAGCTTCCCTATGCTTGCAAGCTCTTGATCCAGGAACTCCAGTCGATGAACATTGTTCCCCGCTTAAAACTAGCTGAAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000428 GO:0001817 GO:0001819 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005666 GO:0005737 GO:0005829 GO:0006139 GO:0006351 GO:0006383 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009553 GO:0009561 GO:0009987 GO:0010467 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0030880 GO:0031347 GO:0031349 GO:0031974 GO:0031981 GO:0032479 GO:0032481 GO:0032501 GO:0032502 GO:0032648 GO:0032728 GO:0032774 GO:0032991 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045088 GO:0045089 GO:0046483 GO:0048229 GO:0048518 GO:0048583 GO:0048584 GO:0048856 GO:0050776 GO:0050778 GO:0050789 GO:0051239 GO:0051240 GO:0055029 GO:0061695 GO:0065007 GO:0070013 GO:0071704 GO:0080134 GO:0090304 GO:0097659 GO:0097747 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169

Pfam Domains

Protein Families

RNA_pol_Rpb2_1 RNA_pol_Rpb2_2 RNA_pol_Rpb2_3 RNA_pol_Rpb2_4 RNA_pol_Rpb2_5 RNA_pol_Rpb2_6 RNA_pol_Rpb2_7

Protein Analysis

1104

Amino Acids

123.23

Weight (kDa)

8.57

Isoelectric Point (pI)

40.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RNA_pol_Rpb2_1	PF04563	58 - 419	1e-52	RNA polymerase beta subunit
RNA_pol_Rpb2_2	PF04561	209 - 385	7.8e-22	RNA polymerase Rpb2, domain 2
RNA_pol_Rpb2_3	PF04565	505 - 569	5.7e-19	RNA polymerase Rpb2, domain 3
RNA_pol_Rpb2_4	PF04566	606 - 666	3.7e-23	RNA polymerase Rpb2, domain 4
RNA_pol_Rpb2_6	PF00562	754 - 853	3.4e-25	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_6	PF00562	854 - 1008	2.5e-70	RNA polymerase Rpb2, domain 6
RNA_pol_Rpb2_7	PF04560	1010 - 1100	7e-31	RNA polymerase Rpb2, domain 7

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0003584)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45140 AT5G45140 AT5G45140
fragaria_vesca	FvH4_5g31720 FvH4_7g03930
malus_domestica	MD14G1029000.v1.1
prunus_persica	Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1 Prupe.2G044100_v2.0.a1
pyrus_communis	pycom14g02710
rosa_chinensis	RchiOBHm_Chr1g0327591 RchiOBHm_Chr7g0227611
rosa_laevigata	RLG00000001632 RLG00000029981
rosa_multiflora	Rmu_co8491793.1_g000001 Rmu_sc0001772.1_g000005 Rmu_ssc0000042.1_g000027
rosa_roxburghii	Rroxscaffold_3G00232000 Rroxscaffold_4G00322550
rosa_rugosa	Rorug01G0068500 Rorug07G0242400
rosa_samantha	Rh1AG085800 Rh1BG068400 Rh1CG083300 Rh1DG090000 Rh7AG395800 Rh7BG377500 Rh7CG414500 Rh7DG391600
rosa_wichuraiana	Rw1G006690 Rw7G032920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	1245, 2479, 2551
AccB1I	GGYRCC	2 cut(s)	854, 3077
AccB7I	CCANNNNNTGG	1 cut(s)	1361
AciI	CCGC	8 cut(s)	11, 26, 111, 126, 1147, 1571, 1869, 3292
AcoI	YGGCCR	1 cut(s)	1982
AcsI	RAATTY	3 cut(s)	137, 1910, 3189
AcuI	CTGAAG	1 cut(s)	1233
AcvI	CACGTG	1 cut(s)	2969
AcyI	GRCGYC	1 cut(s)	264
AfaI	GTAC	6 cut(s)	9, 413, 505, 825, 2059, 2611
AfiI	CCNNNNNNNGG	4 cut(s)	851, 1312, 1361, 3011
AflIII	ACRYGT	1 cut(s)	359
AgsI	TTSAA	6 cut(s)	419, 624, 1198, 1448, 1825, 2013
AhlI	ACTAGT	2 cut(s)	806, 1987
AjnI	CCWGG	5 cut(s)	449, 562, 2256, 2518, 3261
AjuI	GAANNNNNNNTTGG	4 cut(s)	122, 154, 725, 757
Alw26I	GTCTC	3 cut(s)	686, 1548, 1824
AlwNI	CAGNNNCTG	2 cut(s)	1285, 2041
Ama87I	CYCGRG	1 cut(s)	152
AoxI	GGCC	7 cut(s)	1363, 1441, 1549, 1698, 1726, 1982, 2453
ApaI	GGGCCC	1 cut(s)	1730
ApeKI	GCWGC	7 cut(s)	42, 184, 498, 1285, 1871, 1939, 2513
ApoI	RAATTY	3 cut(s)	137, 1910, 3189
Asp700I	GAANNNNTTC	2 cut(s)	1456, 3193
AspLEI	GCGC	2 cut(s)	1086, 3102
AsuHPI	GGTGA	9 cut(s)	178, 557, 952, 1673, 1791, 2179, 2219, 2579, 2792
AsuII	TTCGAA	1 cut(s)	3126
AsuNHI	GCTAGC	1 cut(s)	1519
AvaI	CYCGRG	1 cut(s)	152
AvaII	GGWCC	8 cut(s)	28, 948, 1038, 1601, 2053, 2915, 2947, 2978
BaeGI	GKGCMC	3 cut(s)	554, 859, 1730
BaeI	ACNNNNGTAYC	4 cut(s)	908, 941, 2601, 2634
BanI	GGYRCC	2 cut(s)	854, 3077
BanII	GRGCYC	1 cut(s)	1730
BbrPI	CACGTG	1 cut(s)	2969
BbsI	GAAGAC	1 cut(s)	1229
BbvI	GCAGC	7 cut(s)	54, 196, 485, 1272, 1858, 1951, 2500
BciT130I	CCWGG	5 cut(s)	451, 564, 2258, 2520, 3263
BciVI	GTATCC	1 cut(s)	2232
BclI	TGATCA	1 cut(s)	811
BcoDI	GTCTC	3 cut(s)	686, 1548, 1824
BcuI	ACTAGT	2 cut(s)	806, 1987
BfoI	RGCGCY	1 cut(s)	3103
BfuAI	ACCTGC	3 cut(s)	1245, 2479, 2551
BfuI	GTATCC	1 cut(s)	2232
BglI	GCCNNNNNGGC	2 cut(s)	1629, 1697
BglII	AGATCT	1 cut(s)	735
Bme1390I	CCNGG	5 cut(s)	451, 564, 2258, 2520, 3263
Bme18I	GGWCC	8 cut(s)	28, 948, 1038, 1601, 2053, 2915, 2947, 2978
BmeT110I	CYCGRG	1 cut(s)	152
BmiI	GGNNCC	7 cut(s)	30, 856, 1728, 2054, 2916, 2980, 3079
BmrFI	CCNGG	5 cut(s)	451, 564, 2258, 2520, 3263
BmsI	GCATC	6 cut(s)	1084, 1102, 1482, 2326, 2366, 2475
BmtI	GCTAGC	1 cut(s)	1523
BpiI	GAAGAC	1 cut(s)	1229
BplI	GAGNNNNNCTC	2 cut(s)	1781, 1813
BpmI	CTGGAG	3 cut(s)	585, 1638, 3254
Bpu10I	CCTNAGC	3 cut(s)	284, 1278, 2872
Bpu14I	TTCGAA	1 cut(s)	3126
BpuEI	CTTGAG	1 cut(s)	1172
BsaAI	YACGTR	1 cut(s)	2969
BsaBI	GATNNNNATC	1 cut(s)	750
BsaHI	GRCGYC	1 cut(s)	264
BsaI	GGTCTC	1 cut(s)	1548
BsaJI	CCNNGG	5 cut(s)	21, 129, 1306, 1837, 2519
BsaWI	WCCGGW	1 cut(s)	1027
BsaXI	ACNNNNNCTCC	2 cut(s)	1899, 1929
Bsc4I	CCNNNNNNNGG	4 cut(s)	851, 1312, 1361, 3011
Bse118I	RCCGGY	1 cut(s)	1035
Bse1I	ACTGG	7 cut(s)	223, 1468, 1624, 1680, 2887, 3081, 3271
Bse3DI	GCAATG	3 cut(s)	1881, 1953, 2891
Bse8I	GATNNNNATC	1 cut(s)	750
BseBI	CCWGG	5 cut(s)	451, 564, 2258, 2520, 3263
BseDI	CCNNGG	5 cut(s)	21, 129, 1306, 1837, 2519
BseGI	GGATG	8 cut(s)	968, 1099, 1117, 1126, 1497, 1833, 2341, 2841
BseJI	GATNNNNATC	1 cut(s)	750
BseLI	CCNNNNNNNGG	4 cut(s)	851, 1312, 1361, 3011
BseMI	GCAATG	3 cut(s)	1881, 1953, 2891
BseMII	CTCAG	4 cut(s)	298, 1292, 2886, 3000
BseNI	ACTGG	7 cut(s)	223, 1468, 1624, 1680, 2887, 3081, 3271
BseRI	GAGGAG	3 cut(s)	95, 1736, 1802
BseSI	GKGCMC	3 cut(s)	554, 859, 1730
BseXI	GCAGC	7 cut(s)	54, 196, 485, 1272, 1858, 1951, 2500
BseYI	CCCAGC	4 cut(s)	1895, 2048, 2207, 2282
BsgI	GTGCAG	1 cut(s)	2499
BshFI	GGCC	7 cut(s)	1365, 1443, 1551, 1700, 1728, 1984, 2455
BshNI	GGYRCC	2 cut(s)	854, 3077
BsiHKCI	CYCGRG	1 cut(s)	152
BsiSI	CCGG	3 cut(s)	261, 1028, 1036
BslFI	GGGAC	2 cut(s)	2928, 2964
BslI	CCNNNNNNNGG	4 cut(s)	851, 1312, 1361, 3011
BsmAI	GTCTC	3 cut(s)	686, 1548, 1824
BsmFI	GGGAC	2 cut(s)	2928, 2964
BsmI	GAATGC	3 cut(s)	480, 2050, 2296
BsnI	GGCC	7 cut(s)	1365, 1443, 1551, 1700, 1728, 1984, 2455
Bso31I	GGTCTC	1 cut(s)	1548
BsoBI	CYCGRG	1 cut(s)	152
Bsp119I	TTCGAA	1 cut(s)	3126
Bsp120I	GGGCCC	1 cut(s)	1726
Bsp1286I	GDGCHC	3 cut(s)	554, 859, 1730
Bsp1407I	TGTACA	1 cut(s)	823
BspACI	CCGC	8 cut(s)	11, 26, 111, 126, 1147, 1571, 1869, 3292
BspANI	GGCC	7 cut(s)	1365, 1443, 1551, 1700, 1728, 1984, 2455
BspCNI	CTCAG	4 cut(s)	297, 1291, 2885, 3001
BspHI	TCATGA	3 cut(s)	786, 2346, 2670
BspLI	GGNNCC	7 cut(s)	30, 856, 1728, 2054, 2916, 2980, 3079
BspMI	ACCTGC	3 cut(s)	1245, 2479, 2551
BspOI	GCTAGC	1 cut(s)	1523
BspT104I	TTCGAA	1 cut(s)	3126
BspT107I	GGYRCC	2 cut(s)	854, 3077
BspTNI	GGTCTC	1 cut(s)	1548
BsrDI	GCAATG	3 cut(s)	1881, 1953, 2891
BsrFI	RCCGGY	1 cut(s)	1035
BsrGI	TGTACA	1 cut(s)	823
BsrI	ACTGG	7 cut(s)	223, 1468, 1624, 1680, 2887, 3081, 3271
BssAI	RCCGGY	1 cut(s)	1035
BssECI	CCNNGG	5 cut(s)	21, 129, 1306, 1837, 2519
BssNI	GRCGYC	1 cut(s)	264
BssT1I	CCWWGG	4 cut(s)	21, 129, 1306, 1837
Bst2UI	CCWGG	5 cut(s)	451, 564, 2258, 2520, 3263
Bst4CI	ACNGT	7 cut(s)	57, 712, 983, 1228, 1485, 2299, 2704
Bst6I	CTCTTC	4 cut(s)	832, 938, 1182, 1713
BstACI	GRCGYC	1 cut(s)	264
BstAPI	GCANNNNNTGC	3 cut(s)	919, 1638, 2300
BstAUI	TGTACA	1 cut(s)	823
BstBAI	YACGTR	1 cut(s)	2969
BstBI	TTCGAA	1 cut(s)	3126
BstC8I	GCNNGC	9 cut(s)	71, 240, 915, 1521, 1634, 2898, 2964, 3247, 3251
BstDEI	CTNAG	7 cut(s)	36, 284, 1278, 1687, 2194, 2872, 3009
BstF5I	GGATG	8 cut(s)	968, 1099, 1117, 1126, 1497, 1833, 2341, 2841
BstH2I	RGCGCY	1 cut(s)	3103
BstHHI	GCGC	2 cut(s)	1086, 3102
BstMAI	GTCTC	3 cut(s)	686, 1548, 1824
BstNI	CCWGG	5 cut(s)	451, 564, 2258, 2520, 3263
BstNSI	RCATGY	5 cut(s)	363, 1636, 1671, 2035, 2966
BstSCI	CCNGG	5 cut(s)	449, 562, 2256, 2518, 3261
BstSLI	GKGCMC	3 cut(s)	554, 859, 1730
BstV1I	GCAGC	7 cut(s)	54, 196, 485, 1272, 1858, 1951, 2500
BstV2I	GAAGAC	1 cut(s)	1229
BstX2I	RGATCY	4 cut(s)	735, 745, 841, 1189
BstXI	CCANNNNNNTGG	2 cut(s)	279, 781
BstYI	RGATCY	4 cut(s)	735, 745, 841, 1189
BsuI	GTATCC	1 cut(s)	2232
BsuRI	GGCC	7 cut(s)	1365, 1443, 1551, 1700, 1728, 1984, 2455
BtgZI	GCGATG	1 cut(s)	1130
BtsCI	GGATG	8 cut(s)	968, 1099, 1117, 1126, 1497, 1833, 2341, 2841
BtsI	GCAGTG	1 cut(s)	917
BtsIMutI	CAGTG	5 cut(s)	216, 317, 917, 1631, 2880
BveI	ACCTGC	3 cut(s)	1245, 2479, 2551
Cac8I	GCNNGC	9 cut(s)	71, 240, 915, 1521, 1634, 2898, 2964, 3247, 3251
CaiI	CAGNNNCTG	2 cut(s)	1285, 2041
CciI	TCATGA	3 cut(s)	786, 2346, 2670
CfoI	GCGC	2 cut(s)	1086, 3102
Cfr10I	RCCGGY	1 cut(s)	1035
CseI	GACGC	1 cut(s)	253
CsiI	ACCWGGT	1 cut(s)	2256
Csp6I	GTAC	6 cut(s)	8, 412, 504, 824, 2058, 2610
CviQI	GTAC	6 cut(s)	8, 412, 504, 824, 2058, 2610
DdeI	CTNAG	7 cut(s)	36, 284, 1278, 1687, 2194, 2872, 3009
DraI	TTTAAA	1 cut(s)	1104
EaeI	YGGCCR	1 cut(s)	1982
Eam1104I	CTCTTC	4 cut(s)	832, 938, 1182, 1713
EarI	CTCTTC	4 cut(s)	832, 938, 1182, 1713
EciI	GGCGGA	2 cut(s)	41, 115
Eco130I	CCWWGG	4 cut(s)	21, 129, 1306, 1837
Eco24I	GRGCYC	1 cut(s)	1730
Eco31I	GGTCTC	1 cut(s)	1548
Eco47I	GGWCC	8 cut(s)	28, 948, 1038, 1601, 2053, 2915, 2947, 2978
Eco57I	CTGAAG	1 cut(s)	1233
Eco72I	CACGTG	1 cut(s)	2969
Eco88I	CYCGRG	1 cut(s)	152
EcoO109I	RGGNCCY	2 cut(s)	1726, 1727
EcoRII	CCWGG	5 cut(s)	449, 562, 2256, 2518, 3261
EcoT14I	CCWWGG	4 cut(s)	21, 129, 1306, 1837
EcoT22I	ATGCAT	2 cut(s)	1061, 2964
EcoT38I	GRGCYC	1 cut(s)	1730
ErhI	CCWWGG	4 cut(s)	21, 129, 1306, 1837
FalI	AAGNNNNNCTT	2 cut(s)	603, 635
FaqI	GGGAC	2 cut(s)	2928, 2964
FauI	CCCGC	1 cut(s)	3299
FbaI	TGATCA	1 cut(s)	811
FokI	GGATG	8 cut(s)	955, 1106, 1124, 1133, 1504, 1820, 2348, 2848
FriOI	GRGCYC	1 cut(s)	1730
GlaI	GCGC	2 cut(s)	1085, 3101
GsaI	CCCAGC	4 cut(s)	1899, 2052, 2211, 2286
GsuI	CTGGAG	3 cut(s)	585, 1638, 3254
HaeII	RGCGCY	1 cut(s)	3103
HaeIII	GGCC	7 cut(s)	1365, 1443, 1551, 1700, 1728, 1984, 2455
HapII	CCGG	3 cut(s)	261, 1028, 1036
HgaI	GACGC	1 cut(s)	253
HhaI	GCGC	2 cut(s)	1086, 3102
Hin1I	GRCGYC	1 cut(s)	264
Hin6I	GCGC	2 cut(s)	1084, 3100
HinP1I	GCGC	2 cut(s)	1084, 3100
HincII	GTYRAC	3 cut(s)	214, 1408, 2107
HindII	GTYRAC	3 cut(s)	214, 1408, 2107
HindIII	AAGCTT	2 cut(s)	3233, 3309
HpaI	GTTAAC	1 cut(s)	2107
HpaII	CCGG	3 cut(s)	261, 1028, 1036
HphI	GGTGA	9 cut(s)	178, 557, 952, 1673, 1791, 2179, 2219, 2579, 2792
Hpy166II	GTNNAC	8 cut(s)	214, 1231, 1408, 1601, 1673, 1951, 2107, 2783
Hpy8I	GTNNAC	8 cut(s)	214, 1231, 1408, 1601, 1673, 1951, 2107, 2783
Hpy99I	CGWCG	1 cut(s)	269
HpyAV	CCTTC	7 cut(s)	413, 589, 649, 784, 887, 975, 1652
HpyCH4III	ACNGT	7 cut(s)	57, 712, 983, 1228, 1485, 2299, 2704
HpyCH4IV	ACGT	5 cut(s)	380, 414, 663, 2647, 2968
HpyF3I	CTNAG	7 cut(s)	36, 284, 1278, 1687, 2194, 2872, 3009
HpySE526I	ACGT	5 cut(s)	380, 414, 663, 2647, 2968
Hsp92I	GRCGYC	1 cut(s)	264
HspAI	GCGC	2 cut(s)	1084, 3100
Ksp22I	TGATCA	1 cut(s)	811
KspAI	GTTAAC	1 cut(s)	2107
LmnI	GCTCC	7 cut(s)	355, 394, 1282, 1789, 2038, 2279, 3052
Lsp1109I	GCAGC	7 cut(s)	54, 196, 485, 1272, 1858, 1951, 2500
LweI	GCATC	6 cut(s)	1084, 1102, 1482, 2326, 2366, 2475
MabI	ACCWGGT	1 cut(s)	2256
MaeII	ACGT	5 cut(s)	380, 414, 663, 2647, 2968
MfeI	CAATTG	1 cut(s)	2613
MflI	RGATCY	4 cut(s)	735, 745, 841, 1189
MhlI	GDGCHC	3 cut(s)	554, 859, 1730
MlyI	GAGTC	6 cut(s)	11, 188, 408, 1286, 2025, 2994
MmeI	TCCRAC	1 cut(s)	814
Mph1103I	ATGCAT	2 cut(s)	1061, 2964
MroXI	GAANNNNTTC	2 cut(s)	1456, 3193
MslI	CAYNNNNRTG	3 cut(s)	644, 779, 2967
MspA1I	CMGCKG	1 cut(s)	1871
MspI	CCGG	3 cut(s)	261, 1028, 1036
MspR9I	CCNGG	5 cut(s)	451, 564, 2258, 2520, 3263
MunI	CAATTG	1 cut(s)	2613
Mva1269I	GAATGC	3 cut(s)	480, 2050, 2296
MvaI	CCWGG	5 cut(s)	451, 564, 2258, 2520, 3263
NheI	GCTAGC	1 cut(s)	1519
NlaIV	GGNNCC	7 cut(s)	30, 856, 1728, 2054, 2916, 2980, 3079
NmuCI	GTSAC	7 cut(s)	334, 425, 445, 1584, 1646, 2078, 2567
NsiI	ATGCAT	2 cut(s)	1061, 2964
NspI	RCATGY	5 cut(s)	363, 1636, 1671, 2035, 2966
NspV	TTCGAA	1 cut(s)	3126
PaeI	GCATGC	2 cut(s)	1636, 2966
PagI	TCATGA	3 cut(s)	786, 2346, 2670
PciI	ACATGT	1 cut(s)	359
PcsI	WCGNNNNNNNCGW	1 cut(s)	2088
PctI	GAATGC	3 cut(s)	480, 2050, 2296
PdmI	GAANNNNTTC	2 cut(s)	1456, 3193
PfeI	GAWTC	6 cut(s)	222, 524, 769, 932, 2474, 2877
PflMI	CCANNNNNTGG	1 cut(s)	1361
PfoI	TCCNGGA	2 cut(s)	562, 3261
PleI	GAGTC	6 cut(s)	11, 188, 407, 1286, 2024, 2993
PmaCI	CACGTG	1 cut(s)	2969
PmlI	CACGTG	1 cut(s)	2969
PpsI	GAGTC	6 cut(s)	11, 188, 407, 1286, 2024, 2993
Ppu21I	YACGTR	1 cut(s)	2969
PscI	ACATGT	1 cut(s)	359
Psp6I	CCWGG	5 cut(s)	449, 562, 2256, 2518, 3261
PspCI	CACGTG	1 cut(s)	2969
PspFI	CCCAGC	4 cut(s)	1895, 2048, 2207, 2282
PspGI	CCWGG	5 cut(s)	449, 562, 2256, 2518, 3261
PspN4I	GGNNCC	7 cut(s)	30, 856, 1728, 2054, 2916, 2980, 3079
PspOMI	GGGCCC	1 cut(s)	1726
PsrI	GAACNNNNNNTAC	4 cut(s)	981, 1013, 1444, 1476
PstNI	CAGNNNCTG	2 cut(s)	1285, 2041
PsuI	RGATCY	4 cut(s)	735, 745, 841, 1189
RsaI	GTAC	6 cut(s)	9, 413, 505, 825, 2059, 2611
RsaNI	GTAC	6 cut(s)	8, 412, 504, 824, 2058, 2610
RseI	CAYNNNNRTG	3 cut(s)	644, 779, 2967
SchI	GAGTC	6 cut(s)	11, 188, 408, 1286, 2025, 2994
ScrFI	CCNGG	5 cut(s)	451, 564, 2258, 2520, 3263
SduI	GDGCHC	3 cut(s)	554, 859, 1730
SexAI	ACCWGGT	1 cut(s)	2256
SfaNI	GCATC	6 cut(s)	1084, 1102, 1482, 2326, 2366, 2475
SfuI	TTCGAA	1 cut(s)	3126
SinI	GGWCC	8 cut(s)	28, 948, 1038, 1601, 2053, 2915, 2947, 2978
SmiMI	CAYNNNNRTG	3 cut(s)	644, 779, 2967
SmlI	CTYRAG	1 cut(s)	1151
SmoI	CTYRAG	1 cut(s)	1151
SpeI	ACTAGT	2 cut(s)	806, 1987
SphI	GCATGC	2 cut(s)	1636, 2966
SsiI	CCGC	8 cut(s)	11, 26, 111, 126, 1147, 1571, 1869, 3292
SspI	AATATT	1 cut(s)	3220
StyD4I	CCNGG	5 cut(s)	449, 562, 2256, 2518, 3261
StyI	CCWWGG	4 cut(s)	21, 129, 1306, 1837
TaaI	ACNGT	7 cut(s)	57, 712, 983, 1228, 1485, 2299, 2704
TaiI	ACGT	5 cut(s)	383, 417, 666, 2650, 2971
TatI	WGTACW	1 cut(s)	823
TauI	GCSGC	3 cut(s)	113, 1150, 1871
TfiI	GAWTC	6 cut(s)	222, 524, 769, 932, 2474, 2877
TscAI	CASTG	5 cut(s)	223, 324, 924, 1631, 2887
TseFI	GTSAC	7 cut(s)	334, 425, 445, 1584, 1646, 2078, 2567
TseI	GCWGC	7 cut(s)	42, 184, 498, 1285, 1871, 1939, 2513
Tsp45I	GTSAC	7 cut(s)	334, 425, 445, 1584, 1646, 2078, 2567
TspGWI	ACGGA	3 cut(s)	505, 2045, 2208
TspRI	CASTG	5 cut(s)	223, 324, 924, 1631, 2887
Van91I	CCANNNNNTGG	1 cut(s)	1361
VpaK11BI	GGWCC	8 cut(s)	28, 948, 1038, 1601, 2053, 2915, 2947, 2978
XapI	RAATTY	3 cut(s)	137, 1910, 3189
XceI	RCATGY	5 cut(s)	363, 1636, 1671, 2035, 2966
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1810
XmnI	GAANNNNTTC	2 cut(s)	1456, 3193
Zsp2I	ATGCAT	2 cut(s)	1061, 2964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.