AT5G45760

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008398
Physical Location & Seq
Forward (+)
18561033 .. 18563202
2170 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G45760.2

Sequence Viewer

Length: 1083 bp
ATGGCTACGGCAATGGAAGAGCCAAGGAAGCTGAGAGGCCACAAATCAACTGCCACTTGTTGTATAGCTTCTCGTGACCGGCCTGGAATTGTTCTCACTTCCGGCGAGGATGGTTGCATTTGCTGGTTTGATTTGCGATGTAAAGATGTTCAATTCATCATCGATGTTGGAACAGAACCAGTTTCATCTCTTTGTTTCAAGACAGGTAATGAGAACATTCTCTATGCTTCTTGTGGAAACGAAATCAAGTCTTTCGATGTTCATACGCTAAGTGCTACTTCTTGGAAGCCATTAGAGAGTTACAATTACAACAAAGATGAAGTTAACCAGGTAGTATGTAACGGAAGGTCGTCTTTTCTTGCTTCTGCAGATGATTCCGGCGATGTTAAGATCATCGACCTTGGTCAGAAATGCCTTTATAAAACCCTTAGAGCTGGTCACACAAGTATATGTAGTTCTGTACAATTCATTCCATGGAGACCTTGGGAAGTTATAACTGGTGGACTTGATTCAAAGCTTGTTTTATGGGATTTCTCAAAAGGTCGCTCGCAAAAGATTATAGACTTTGGATCTGATACAAATAATAACTCGGGACAATGCCTAAACCCTGCGTTTGTTCACTCAATAGCAATACCGGAAATGGACATGGTTGATAAGTTAGACAAGATATGTGCGGTTGCTAGAGGTGATGGAATTGTCAATTTGATCAATATCGAATCAGAACTTTCCCGAAAAGGAACATCATCAAAAGGAAGTAGCAGCAGCAGCAGCAACAATGTGGTCAAAAGGGTTTGTTTAGACTACTCTGTTGGTGGACACAATTCTGCTGTTTCTTGTGTAGCCTTTTCTCTGTTTCAGGAGAAAGGTAGATTCTTAATCTCTGGTGGTAATGATAAAACAGTCAAAATCTGGGATTGCTTTAAATGTCTTGATCCAAACAACAACAACAATAACAGTGATCTCCTCCATTTGAATATTAACCTAAGCAAGAAGGTGAATTGGCTTTGCACGAATCAATCTGATTCTGAGAATCTTGTAGTGTGTGACACAACAAGAGTAGTTAAGGTTTACTCAATCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

360

Amino Acids

39.5

Weight (kDa)

6.59

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 9 - 158 1.4e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 18 - 158 4.9e-07 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 19 - 161 8.6e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 243 - 279 2.3e-08 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 247 - 333 5.4e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 420, 494
AciI CCGC 1 cut(s) 674
AclWI GGATC 2 cut(s) 577, 926
AfaI GTAC 1 cut(s) 462
AgsI TTSAA 4 cut(s) 152, 199, 513, 973
AjnI CCWGG 2 cut(s) 82, 327
AluBI AGCT 4 cut(s) 31, 68, 434, 517
AluI AGCT 4 cut(s) 31, 68, 434, 517
Alw26I GTCTC 1 cut(s) 472
AlwI GGATC 2 cut(s) 577, 926
Ama87I CYCGRG 1 cut(s) 589
AoxI GGCC 2 cut(s) 37, 80
ApeKI GCWGC 4 cut(s) 759, 762, 765, 768
AsuHPI GGTGA 2 cut(s) 698, 1006
AvaI CYCGRG 1 cut(s) 589
BauI CACGAG 1 cut(s) 72
BbvI GCAGC 4 cut(s) 771, 774, 777, 780
BccI CCATC 2 cut(s) 104, 683
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 2 cut(s) 84, 329
BclI TGATCA 1 cut(s) 705
BcoDI GTCTC 1 cut(s) 472
BfaI CTAG 1 cut(s) 681
BfmI CTRYAG 1 cut(s) 366
BisI GCNGC 4 cut(s) 760, 763, 766, 769
BlsI GCNGC 4 cut(s) 761, 764, 767, 770
Bme1390I CCNGG 2 cut(s) 84, 329
BmeT110I CYCGRG 1 cut(s) 589
BmrFI CCNGG 2 cut(s) 84, 329
BoxI GACNNNNGTC 1 cut(s) 402
Bpu10I CCTNAGC 1 cut(s) 983
Bsa29I ATCGAT 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 710
BsaI GGTCTC 1 cut(s) 472
BsaJI CCNNGG 4 cut(s) 23, 400, 473, 482
BsaWI WCCGGW 1 cut(s) 634
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 2 cut(s) 179, 502
Bse3DI GCAATG 1 cut(s) 18
Bse8I GATNNNNATC 1 cut(s) 710
BseBI CCWGG 2 cut(s) 84, 329
BseCI ATCGAT 1 cut(s) 162
BseDI CCNNGG 4 cut(s) 23, 400, 473, 482
BseGI GGATG 1 cut(s) 115
BseJI GATNNNNATC 1 cut(s) 710
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 2 cut(s) 23, 1017
BseNI ACTGG 2 cut(s) 179, 502
BseRI GAGGAG 1 cut(s) 953
BseXI GCAGC 4 cut(s) 771, 774, 777, 780
BshFI GGCC 2 cut(s) 39, 82
BshVI ATCGAT 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 589
BsiSI CCGG 4 cut(s) 79, 102, 378, 635
BslFI GGGAC 1 cut(s) 606
BsmAI GTCTC 1 cut(s) 472
BsmFI GGGAC 1 cut(s) 606
BsnI GGCC 2 cut(s) 39, 82
Bso31I GGTCTC 1 cut(s) 472
BsoBI CYCGRG 1 cut(s) 589
Bsp1407I TGTACA 1 cut(s) 460
Bsp143I GATC 5 cut(s) 390, 569, 705, 931, 958
Bsp19I CCATGG 1 cut(s) 473
BspACI CCGC 1 cut(s) 674
BspANI GGCC 2 cut(s) 39, 82
BspCNI CTCAG 2 cut(s) 24, 1018
BspDI ATCGAT 1 cut(s) 162
BspMAI CTGCAG 1 cut(s) 370
BspPI GGATC 2 cut(s) 577, 926
BspQI GCTCTTC 1 cut(s) 12
BspTNI GGTCTC 1 cut(s) 472
BsrDI GCAATG 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 78
BsrGI TGTACA 1 cut(s) 460
BsrI ACTGG 2 cut(s) 179, 502
BssAI RCCGGY 1 cut(s) 78
BssECI CCNNGG 4 cut(s) 23, 400, 473, 482
BssMI GATC 5 cut(s) 390, 569, 705, 931, 958
BssSI CACGAG 1 cut(s) 72
BssT1I CCWWGG 4 cut(s) 23, 400, 473, 482
Bst2BI CACGAG 1 cut(s) 72
Bst2UI CCWGG 2 cut(s) 84, 329
Bst4CI ACNGT 2 cut(s) 901, 956
Bst6I CTCTTC 1 cut(s) 12
BstAUI TGTACA 1 cut(s) 460
BstC8I GCNNGC 1 cut(s) 548
BstDEI CTNAG 5 cut(s) 32, 269, 428, 983, 1026
BstDSI CCRYGG 1 cut(s) 473
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 5 cut(s) 393, 572, 708, 934, 961
BstMAI GTCTC 1 cut(s) 472
BstMBI GATC 5 cut(s) 390, 569, 705, 931, 958
BstMWI GCNNNNNNNGC 3 cut(s) 28, 765, 768
BstNI CCWGG 2 cut(s) 84, 329
BstPAI GACNNNNGTC 1 cut(s) 402
BstSCI CCNGG 2 cut(s) 82, 327
BstSFI CTRYAG 1 cut(s) 366
BstV1I GCAGC 4 cut(s) 771, 774, 777, 780
BstX2I RGATCY 1 cut(s) 569
BstYI RGATCY 1 cut(s) 569
Bsu15I ATCGAT 1 cut(s) 162
BsuRI GGCC 2 cut(s) 39, 82
BsuTUI ATCGAT 1 cut(s) 162
BtgI CCRYGG 1 cut(s) 473
BtgZI GCGATG 2 cut(s) 151, 396
BtsCI GGATG 1 cut(s) 115
BtsIMutI CAGTG 1 cut(s) 961
Cac8I GCNNGC 1 cut(s) 548
Cfr10I RCCGGY 1 cut(s) 78
ClaI ATCGAT 1 cut(s) 162
CsiI ACCWGGT 1 cut(s) 327
Csp6I GTAC 1 cut(s) 461
CviAII CATG 2 cut(s) 474, 646
CviQI GTAC 1 cut(s) 461
DdeI CTNAG 5 cut(s) 32, 269, 428, 983, 1026
DpnI GATC 5 cut(s) 392, 571, 707, 933, 960
DpnII GATC 5 cut(s) 390, 569, 705, 931, 958
DraI TTTAAA 1 cut(s) 922
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 4 cut(s) 23, 400, 473, 482
Eco31I GGTCTC 1 cut(s) 472
Eco88I CYCGRG 1 cut(s) 589
EcoRII CCWGG 2 cut(s) 82, 327
EcoT14I CCWWGG 4 cut(s) 23, 400, 473, 482
ErhI CCWWGG 4 cut(s) 23, 400, 473, 482
FaeI CATG 2 cut(s) 477, 649
FalI AAGNNNNNCTT 4 cut(s) 262, 294, 337, 369
FaqI GGGAC 1 cut(s) 606
FatI CATG 2 cut(s) 473, 645
FbaI TGATCA 1 cut(s) 705
Fnu4HI GCNGC 4 cut(s) 760, 763, 766, 769
FokI GGATG 1 cut(s) 122
Fsp4HI GCNGC 4 cut(s) 760, 763, 766, 769
FspBI CTAG 1 cut(s) 681
GluI GCNGC 4 cut(s) 760, 763, 766, 769
HaeIII GGCC 2 cut(s) 39, 82
HapII CCGG 4 cut(s) 79, 102, 378, 635
Hin1II CATG 2 cut(s) 477, 649
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HindIII AAGCTT 1 cut(s) 515
HinfI GANTC 7 cut(s) 374, 509, 716, 870, 1012, 1022, 1030
HpaI GTTAAC 1 cut(s) 325
HpaII CCGG 4 cut(s) 79, 102, 378, 635
HphI GGTGA 2 cut(s) 698, 1006
Hpy166II GTNNAC 5 cut(s) 325, 503, 619, 815, 1069
Hpy188I TCNGA 5 cut(s) 408, 574, 721, 1021, 1027
Hpy188III TCNNGA 6 cut(s) 74, 199, 591, 729, 857, 929
Hpy8I GTNNAC 5 cut(s) 325, 503, 619, 815, 1069
HpyAV CCTTC 2 cut(s) 339, 985
HpyCH4III ACNGT 2 cut(s) 901, 956
HpyCH4V TGCA 3 cut(s) 117, 368, 1008
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 765, 768
HpyF3I CTNAG 5 cut(s) 32, 269, 428, 983, 1026
Hsp92II CATG 2 cut(s) 477, 649
Ksp22I TGATCA 1 cut(s) 705
KspAI GTTAAC 1 cut(s) 325
Kzo9I GATC 5 cut(s) 390, 569, 705, 931, 958
LguI GCTCTTC 1 cut(s) 12
Lsp1109I GCAGC 4 cut(s) 771, 774, 777, 780
MabI ACCWGGT 1 cut(s) 327
MaeI CTAG 1 cut(s) 681
MaeIII GTNAC 5 cut(s) 74, 299, 338, 437, 1043
MalI GATC 5 cut(s) 392, 571, 707, 933, 960
MboI GATC 5 cut(s) 390, 569, 705, 931, 958
MboII GAAGA 1 cut(s) 29
MflI RGATCY 1 cut(s) 569
MluCI AATT 8 cut(s) 87, 152, 304, 464, 693, 700, 820, 997
MmeI TCCRAC 1 cut(s) 148
MnlI CCTC 4 cut(s) 29, 100, 677, 974
MseI TTAA 7 cut(s) 324, 387, 875, 921, 978, 1062, 1081
MspI CCGG 4 cut(s) 79, 102, 378, 635
MspR9I CCNGG 2 cut(s) 84, 329
MvaI CCWGG 2 cut(s) 84, 329
MwoI GCNNNNNNNGC 3 cut(s) 28, 765, 768
NcoI CCATGG 1 cut(s) 473
NdeII GATC 5 cut(s) 390, 569, 705, 931, 958
NlaIII CATG 2 cut(s) 477, 649
NmuCI GTSAC 3 cut(s) 74, 437, 1043
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 7 cut(s) 374, 509, 716, 870, 1012, 1022, 1030
PkrI GCNGC 4 cut(s) 761, 764, 767, 770
PshAI GACNNNNGTC 1 cut(s) 402
PsiI TTATAA 2 cut(s) 420, 494
Psp6I CCWGG 2 cut(s) 82, 327
PspGI CCWGG 2 cut(s) 82, 327
PsrI GAACNNNNNNTAC 2 cut(s) 439, 471
PstI CTGCAG 1 cut(s) 370
PsuI RGATCY 1 cut(s) 569
RsaI GTAC 1 cut(s) 462
RsaNI GTAC 1 cut(s) 461
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 7 cut(s) 324, 387, 875, 921, 978, 1062, 1081
SatI GCNGC 4 cut(s) 760, 763, 766, 769
Sau3AI GATC 5 cut(s) 390, 569, 705, 931, 958
ScrFI CCNGG 2 cut(s) 84, 329
SexAI ACCWGGT 1 cut(s) 327
SfcI CTRYAG 1 cut(s) 366
Sse9I AATT 8 cut(s) 87, 152, 304, 464, 693, 700, 820, 997
SsiI CCGC 1 cut(s) 674
SspI AATATT 1 cut(s) 976
SspMI CTAG 1 cut(s) 681
StyD4I CCNGG 2 cut(s) 82, 327
StyI CCWWGG 4 cut(s) 23, 400, 473, 482
TaaI ACNGT 2 cut(s) 901, 956
TaqI TCGA 4 cut(s) 162, 255, 396, 714
TasI AATT 8 cut(s) 87, 152, 304, 464, 693, 700, 820, 997
TatI WGTACW 1 cut(s) 460
TfiI GAWTC 7 cut(s) 374, 509, 716, 870, 1012, 1022, 1030
Tru1I TTAA 7 cut(s) 324, 387, 875, 921, 978, 1062, 1081
Tru9I TTAA 7 cut(s) 324, 387, 875, 921, 978, 1062, 1081
TscAI CASTG 1 cut(s) 961
TseFI GTSAC 3 cut(s) 74, 437, 1043
TseI GCWGC 4 cut(s) 759, 762, 765, 768
Tsp45I GTSAC 3 cut(s) 74, 437, 1043
TspDTI ATGAA 5 cut(s) 145, 174, 251, 333, 457
TspGWI ACGGA 1 cut(s) 357
TspRI CASTG 1 cut(s) 961
XcmI CCANNNNNNNNNTGG 1 cut(s) 480
XspI CTAG 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.