Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008398

Physical Location & Seq

Genomic Coordinates Forward (+)

43087482 .. 43091053

Gene Structure 3572 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1296500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1116 bp

ATGGCGGACCCGAAACCAAGGCGACTGAAGGGTCACAAGGCCACCGCCACGTGCTGCATCGCCTCAGCCGCTACACCCGGCCTCGTCGCCACCTCCGGCGAGGATGGCTGCGTTTGCTGGTTCGATATGCGATGCAAAGATGCGATTGATATTATGGAGGTGGGAGAGGAACCCATTTCATCCTTATGTTTCAGGCCGGGAAATGAAAATATGATATATGTTGCATCCGGGAAGCAAGTCAAGAGCTTTGATGTCCGTCTGGGGAGTTCATGCTCGTGGAAGCCCCTGGAGAGCTTTGATTATAACAAAGAAGAGATAAATCAGATTGCATGCAGCTCTAAGTCCTCTTTTCTTGCTGCTCCCGATGACGGCGGTGAAATTAAGATTATTGACATTCGCCAGAAACGAATTTACAAAACATTAAGAGATGGCCATACAAGTATTTGTAGCACCGTGCAATTCCTTCCTTGGAAACCTTGGGAAGTTATCACGGGAGGTCTTGATTCAAAGCTTGTAATGTGGGATTTCTCCAAGGGGCGCCCGAACAAAATTATGAATTTTGATTTGCCTGATGCAAATAATGGCGAGGCCAAGGAACAGTGTTTTAATCCTGCTTTTGTTCATTCAATAGCAGTTCCAGAAATGGATATGTTAGACAAATCAGACAAGGTTTGCATTGTGGCTAGGGGTGATGGTGCAGTTGATGTAATTAATATAGAAGCTGAACTTGCTCCCTTGAGGTCACAAGGTTCTAAAAAACCTCAGAAAGGATCTCAATCAAGATCGAAAGGTACTGATCTAGAAAACCCAGATCGAGAAGGAAGGAAAAGGTTGCATTTGGATTACTTATCCGGCGGTCACACTGCTGCTGTCTCTTGTGTGGCATTTTCGTTATTTGGAGATAGAGGAAGATTTATAATATCAGGTGGAAATGATAAGTTGGTAAAGGTCTGGGATTGGACCAAGTGTCTTGATGAAGGTAGCAGCAGCGATCTTCTACAGTTGAATATCAACCACAGCAAAAAAGTCAACTGGCTCTGTACCACTCCGGCTGATACGGAAAACCTAGTTGTTTGTGATACAACAAAGGTGGTAAAGGTTTATTCTGTTGCCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005886 GO:0005975 GO:0005976 GO:0006073 GO:0006950 GO:0006952 GO:0006970 GO:0007049 GO:0008150 GO:0008152 GO:0008194 GO:0009058 GO:0009059 GO:0009250 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009832 GO:0009833 GO:0009834 GO:0009987 GO:0010035 GO:0012505 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0016760 GO:0022402 GO:0030243 GO:0030244 GO:0030312 GO:0033692 GO:0034637 GO:0034645 GO:0035251 GO:0042221 GO:0042546 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0046527 GO:0050832 GO:0050896 GO:0051273 GO:0051274 GO:0051301 GO:0051704 GO:0051707 GO:0061640 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0098542 GO:1901576 GO:1901700 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt WD40 zf-UDP

Protein Analysis

372

Amino Acids

40.78

Weight (kDa)

6.61

Isoelectric Point (pI)

35.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_THOC3	PF25174	8 - 179	1.4e-15	THOC3 beta-propeller domain
WD40_Prp19	PF24814	14 - 156	1.6e-08	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	16 - 183	5.3e-12	CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	16 - 176	8.6e-08	WDR36/Utp21 first beta-propeller
Beta-prop_EML	PF23409	29 - 193	7.8e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR3_1st	PF25173	72 - 179	5.6e-07	WDR3 first beta-propeller domain
Beta-prop_WDR5	PF25175	92 - 185	3e-10	WDR5 beta-propeller domain
WD40_WDHD1_1st	PF24817	104 - 213	6.8e-06	WDHD1 first WD40 domain
WD40	PF00400	286 - 319	8.3e-07	WD domain, G-beta repeat
Beta-prop_THOC3	PF25174	287 - 370	4.6e-07	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008398)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G45760 AT5G45760
fragaria_vesca	FvH4_1g07520 FvH4_1g07520 FvH4_1g07520 FvH4_3g07410
malus_domestica	MD05G1296500.v1.1
prunus_persica	Prupe.4G065800_v2.0.a1
pyrus_communis	pycom05g27500
rosa_chinensis	RchiOBHm_Chr5g0011451 RchiOBHm_Chr5g0011471
rosa_laevigata	RLG00000031836
rosa_multiflora	Rmu_co8262969.1_g000001 Rmu_sc0009001.1_g000002 Rmu_sc0009975.1_g000020
rosa_roxburghii	Rroxscaffold_1G00064980 Rroxscaffold_1G00064990
rosa_rugosa	Rorug04G0457100
rosa_samantha	Rh5AG089000 Rh5BG084200 Rh5CG097700 Rh5CG097900 Rh5DG084200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	303, 917
AasI	GACNNNNNNGTC	1 cut(s)	30
AccB1I	GGYRCC	1 cut(s)	537
AciI	CCGC	5 cut(s)	5, 45, 69, 372, 855
AclWI	GGATC	1 cut(s)	778
AcoI	YGGCCR	1 cut(s)	430
AcsI	RAATTY	2 cut(s)	408, 556
AcuI	CTGAAG	1 cut(s)	47
AcvI	CACGTG	1 cut(s)	51
AcyI	GRCGYC	1 cut(s)	538
AfaI	GTAC	2 cut(s)	793, 1042
AfiI	CCNNNNNNNGG	2 cut(s)	368, 767
AgsI	TTSAA	3 cut(s)	507, 627, 1006
AhdI	GACNNNNNGTC	1 cut(s)	966
AjnI	CCWGG	1 cut(s)	285
AluBI	AGCT	5 cut(s)	246, 294, 336, 511, 722
AluI	AGCT	5 cut(s)	246, 294, 336, 511, 722
Alw26I	GTCTC	1 cut(s)	877
AlwI	GGATC	1 cut(s)	778
AoxI	GGCC	5 cut(s)	39, 79, 194, 430, 588
ApeKI	GCWGC	7 cut(s)	54, 108, 333, 356, 866, 984, 987
ApoI	RAATTY	2 cut(s)	408, 556
AseI	ATTAAT	1 cut(s)	711
AspLEI	GCGC	1 cut(s)	540
AspS9I	GGNCC	2 cut(s)	7, 960
AsuC2I	CCSGG	3 cut(s)	78, 198, 229
AsuHPI	GGTGA	2 cut(s)	386, 701
AvaII	GGWCC	2 cut(s)	7, 960
BalI	TGGCCA	1 cut(s)	432
BanI	GGYRCC	1 cut(s)	537
BauI	CACGAG	1 cut(s)	274
BbrPI	CACGTG	1 cut(s)	51
BbvCI	CCTCAGC	1 cut(s)	64
BbvI	GCAGC	7 cut(s)	41, 95, 343, 345, 853, 996, 999
BccI	CCATC	3 cut(s)	98, 422, 686
BceAI	ACGGC	1 cut(s)	385
BciT130I	CCWGG	1 cut(s)	287
BcnI	CCSGG	3 cut(s)	78, 198, 229
BcoDI	GTCTC	1 cut(s)	877
BfaI	CTAG	4 cut(s)	684, 800, 1067, 1114
BfmI	CTRYAG	1 cut(s)	998
BfoI	RGCGCY	1 cut(s)	541
BisI	GCNGC	8 cut(s)	55, 69, 109, 334, 357, 867, 985, 988
BlsI	GCNGC	8 cut(s)	56, 70, 110, 335, 358, 868, 986, 989
Bme1390I	CCNGG	4 cut(s)	78, 198, 229, 287
Bme18I	GGWCC	2 cut(s)	7, 960
BmeRI	GACNNNNNGTC	1 cut(s)	966
BmgT120I	GGNCC	2 cut(s)	7, 960
BmiI	GGNNCC	3 cut(s)	9, 171, 539
BmrFI	CCNGG	4 cut(s)	78, 198, 229, 287
BmsI	GCATC	5 cut(s)	66, 122, 130, 233, 562
BpmI	CTGGAG	1 cut(s)	308
Bpu10I	CCTNAGC	1 cut(s)	64
BpuEI	CTTGAG	1 cut(s)	757
BpuMI	CCSGG	3 cut(s)	78, 198, 229
BsaAI	YACGTR	1 cut(s)	51
BsaBI	GATNNNNATC	1 cut(s)	775
BsaHI	GRCGYC	1 cut(s)	538
BsaJI	CCNNGG	6 cut(s)	17, 285, 467, 476, 531, 591
Bsc4I	CCNNNNNNNGG	2 cut(s)	368, 767
Bse1I	ACTGG	1 cut(s)	1037
Bse8I	GATNNNNATC	1 cut(s)	775
BseBI	CCWGG	1 cut(s)	287
BseDI	CCNNGG	6 cut(s)	17, 285, 467, 476, 531, 591
BseGI	GGATG	3 cut(s)	109, 179, 224
BseJI	GATNNNNATC	1 cut(s)	775
BseLI	CCNNNNNNNGG	2 cut(s)	368, 767
BseMII	CTCAG	2 cut(s)	78, 776
BseNI	ACTGG	1 cut(s)	1037
BseXI	GCAGC	7 cut(s)	41, 95, 343, 345, 853, 996, 999
BsgI	GTGCAG	1 cut(s)	717
BshFI	GGCC	5 cut(s)	41, 81, 196, 432, 590
BshNI	GGYRCC	1 cut(s)	537
BsiSI	CCGG	6 cut(s)	78, 96, 197, 228, 852, 1049
BslI	CCNNNNNNNGG	2 cut(s)	368, 767
BsmAI	GTCTC	1 cut(s)	877
BsnI	GGCC	5 cut(s)	41, 81, 196, 432, 590
Bsp143I	GATC	5 cut(s)	770, 782, 796, 811, 991
BspACI	CCGC	5 cut(s)	5, 45, 69, 372, 855
BspANI	GGCC	5 cut(s)	41, 81, 196, 432, 590
BspCNI	CTCAG	2 cut(s)	77, 775
BspLI	GGNNCC	3 cut(s)	9, 171, 539
BspPI	GGATC	1 cut(s)	778
BspT107I	GGYRCC	1 cut(s)	537
BsrI	ACTGG	1 cut(s)	1037
BssECI	CCNNGG	6 cut(s)	17, 285, 467, 476, 531, 591
BssMI	GATC	5 cut(s)	770, 782, 796, 811, 991
BssNI	GRCGYC	1 cut(s)	538
BssSI	CACGAG	1 cut(s)	274
BssT1I	CCWWGG	5 cut(s)	17, 467, 476, 531, 591
Bst2BI	CACGAG	1 cut(s)	274
Bst2UI	CCWGG	1 cut(s)	287
Bst4CI	ACNGT	3 cut(s)	454, 600, 1002
Bst6I	CTCTTC	1 cut(s)	306
BstACI	GRCGYC	1 cut(s)	538
BstBAI	YACGTR	1 cut(s)	51
BstC8I	GCNNGC	1 cut(s)	331
BstDEI	CTNAG	3 cut(s)	64, 339, 762
BstENI	CCTNNNNNAGG	1 cut(s)	765
BstF5I	GGATG	3 cut(s)	109, 179, 224
BstH2I	RGCGCY	1 cut(s)	541
BstHHI	GCGC	1 cut(s)	540
BstKTI	GATC	5 cut(s)	773, 785, 799, 814, 994
BstMAI	GTCTC	1 cut(s)	877
BstMBI	GATC	5 cut(s)	770, 782, 796, 811, 991
BstMWI	GCNNNNNNNGC	4 cut(s)	68, 105, 114, 728
BstNI	CCWGG	1 cut(s)	287
BstNSI	RCATGY	1 cut(s)	333
BstSCI	CCNGG	4 cut(s)	76, 196, 227, 285
BstSFI	CTRYAG	1 cut(s)	998
BstV1I	GCAGC	7 cut(s)	41, 95, 343, 345, 853, 996, 999
BstX2I	RGATCY	1 cut(s)	770
BstYI	RGATCY	1 cut(s)	770
BsuRI	GGCC	5 cut(s)	41, 81, 196, 432, 590
BtgZI	GCGATG	2 cut(s)	43, 145
BtsCI	GGATG	3 cut(s)	109, 179, 224
BtsI	GCAGTG	1 cut(s)	861
BtsIMutI	CAGTG	2 cut(s)	605, 861
Cac8I	GCNNGC	1 cut(s)	331
CfoI	GCGC	1 cut(s)	540
Cfr13I	GGNCC	2 cut(s)	7, 960
Csp6I	GTAC	2 cut(s)	792, 1041
CspCI	CAANNNNNGTGG	2 cut(s)	1071, 1106
CviAII	CATG	2 cut(s)	270, 330
CviQI	GTAC	2 cut(s)	792, 1041
DdeI	CTNAG	3 cut(s)	64, 339, 762
DinI	GGCGCC	1 cut(s)	539
DpnI	GATC	5 cut(s)	772, 784, 798, 813, 993
DpnII	GATC	5 cut(s)	770, 782, 796, 811, 991
DrdI	GACNNNNNNGTC	1 cut(s)	30
DriI	GACNNNNNGTC	1 cut(s)	966
DseDI	GACNNNNNNGTC	1 cut(s)	30
EaeI	YGGCCR	1 cut(s)	430
Eam1104I	CTCTTC	1 cut(s)	306
Eam1105I	GACNNNNNGTC	1 cut(s)	966
EarI	CTCTTC	1 cut(s)	306
EciI	GGCGGA	1 cut(s)	20
Eco130I	CCWWGG	5 cut(s)	17, 467, 476, 531, 591
Eco47I	GGWCC	2 cut(s)	7, 960
Eco57I	CTGAAG	1 cut(s)	47
Eco72I	CACGTG	1 cut(s)	51
EcoNI	CCTNNNNNAGG	1 cut(s)	765
EcoRII	CCWGG	1 cut(s)	285
EcoT14I	CCWWGG	5 cut(s)	17, 467, 476, 531, 591
EgeI	GGCGCC	1 cut(s)	539
EheI	GGCGCC	1 cut(s)	539
ErhI	CCWWGG	5 cut(s)	17, 467, 476, 531, 591
FaeI	CATG	2 cut(s)	273, 333
FalI	AAGNNNNNCTT	2 cut(s)	711, 743
FatI	CATG	2 cut(s)	269, 329
Fnu4HI	GCNGC	8 cut(s)	55, 69, 109, 334, 357, 867, 985, 988
FokI	GGATG	3 cut(s)	116, 166, 211
Fsp4HI	GCNGC	8 cut(s)	55, 69, 109, 334, 357, 867, 985, 988
FspBI	CTAG	4 cut(s)	684, 800, 1067, 1114
GlaI	GCGC	1 cut(s)	539
GluI	GCNGC	8 cut(s)	55, 69, 109, 334, 357, 867, 985, 988
GsuI	CTGGAG	1 cut(s)	308
HaeII	RGCGCY	1 cut(s)	541
HaeIII	GGCC	5 cut(s)	41, 81, 196, 432, 590
HapII	CCGG	6 cut(s)	78, 96, 197, 228, 852, 1049
HhaI	GCGC	1 cut(s)	540
Hin1I	GRCGYC	1 cut(s)	538
Hin1II	CATG	2 cut(s)	273, 333
Hin6I	GCGC	1 cut(s)	538
HinP1I	GCGC	1 cut(s)	538
HincII	GTYRAC	1 cut(s)	1030
HindII	GTYRAC	1 cut(s)	1030
HindIII	AAGCTT	1 cut(s)	509
HinfI	GANTC	1 cut(s)	503
HpaII	CCGG	6 cut(s)	78, 96, 197, 228, 852, 1049
HphI	GGTGA	2 cut(s)	386, 701
Hpy166II	GTNNAC	1 cut(s)	1030
Hpy188I	TCNGA	3 cut(s)	324, 664, 765
Hpy188III	TCNNGA	8 cut(s)	241, 362, 500, 638, 780, 800, 815, 971
Hpy8I	GTNNAC	1 cut(s)	1030
Hpy99I	CGWCG	1 cut(s)	89
HpyAV	CCTTC	5 cut(s)	22, 473, 812, 816, 971
HpyCH4III	ACNGT	3 cut(s)	454, 600, 1002
HpyCH4IV	ACGT	1 cut(s)	50
HpyF10VI	GCNNNNNNNGC	4 cut(s)	68, 105, 114, 728
HpyF3I	CTNAG	3 cut(s)	64, 339, 762
HpySE526I	ACGT	1 cut(s)	50
Hsp92I	GRCGYC	1 cut(s)	538
Hsp92II	CATG	2 cut(s)	273, 333
HspAI	GCGC	1 cut(s)	538
KasI	GGCGCC	1 cut(s)	537
Kzo9I	GATC	5 cut(s)	770, 782, 796, 811, 991
LmnI	GCTCC	2 cut(s)	364, 736
Lsp1109I	GCAGC	7 cut(s)	41, 95, 343, 345, 853, 996, 999
LweI	GCATC	5 cut(s)	66, 122, 130, 233, 562
MaeI	CTAG	4 cut(s)	684, 800, 1067, 1114
MaeII	ACGT	1 cut(s)	50
MaeIII	GTNAC	3 cut(s)	32, 741, 857
MalI	GATC	5 cut(s)	772, 784, 798, 813, 993
MboI	GATC	5 cut(s)	770, 782, 796, 811, 991
MboII	GAAGA	3 cut(s)	323, 921, 986
MflI	RGATCY	1 cut(s)	770
MlsI	TGGCCA	1 cut(s)	432
MluCI	AATT	6 cut(s)	378, 408, 458, 549, 556, 708
MluNI	TGGCCA	1 cut(s)	432
Mly113I	GGCGCC	1 cut(s)	538
Mox20I	TGGCCA	1 cut(s)	432
MscI	TGGCCA	1 cut(s)	432
MseI	TTAA	4 cut(s)	381, 422, 606, 711
MslI	CAYNNNNRTG	2 cut(s)	184, 274
Msp20I	TGGCCA	1 cut(s)	432
MspI	CCGG	6 cut(s)	78, 96, 197, 228, 852, 1049
MspR9I	CCNGG	4 cut(s)	78, 198, 229, 287
MvaI	CCWGG	1 cut(s)	287
MwoI	GCNNNNNNNGC	4 cut(s)	68, 105, 114, 728
NarI	GGCGCC	1 cut(s)	538
NciI	CCSGG	3 cut(s)	78, 198, 229
NdeII	GATC	5 cut(s)	770, 782, 796, 811, 991
NlaIII	CATG	2 cut(s)	273, 333
NlaIV	GGNNCC	3 cut(s)	9, 171, 539
NmuCI	GTSAC	3 cut(s)	32, 741, 857
NspI	RCATGY	1 cut(s)	333
PaeI	GCATGC	1 cut(s)	333
PcsI	WCGNNNNNNNCGW	1 cut(s)	403
PfeI	GAWTC	1 cut(s)	503
PfoI	TCCNGGA	1 cut(s)	227
PkrI	GCNGC	8 cut(s)	56, 70, 110, 335, 358, 868, 986, 989
PluTI	GGCGCC	1 cut(s)	541
PmaCI	CACGTG	1 cut(s)	51
PmlI	CACGTG	1 cut(s)	51
Ppu21I	YACGTR	1 cut(s)	51
PshBI	ATTAAT	1 cut(s)	711
PsiI	TTATAA	2 cut(s)	303, 917
Psp6I	CCWGG	1 cut(s)	285
PspCI	CACGTG	1 cut(s)	51
PspGI	CCWGG	1 cut(s)	285
PspN4I	GGNNCC	3 cut(s)	9, 171, 539
PspPI	GGNCC	2 cut(s)	7, 960
PsuI	RGATCY	1 cut(s)	770
RsaI	GTAC	2 cut(s)	793, 1042
RsaNI	GTAC	2 cut(s)	792, 1041
RseI	CAYNNNNRTG	2 cut(s)	184, 274
SaqAI	TTAA	4 cut(s)	381, 422, 606, 711
SatI	GCNGC	8 cut(s)	55, 69, 109, 334, 357, 867, 985, 988
Sau3AI	GATC	5 cut(s)	770, 782, 796, 811, 991
Sau96I	GGNCC	2 cut(s)	7, 960
ScrFI	CCNGG	4 cut(s)	78, 198, 229, 287
SfaNI	GCATC	5 cut(s)	66, 122, 130, 233, 562
SfcI	CTRYAG	1 cut(s)	998
SfoI	GGCGCC	1 cut(s)	539
SinI	GGWCC	2 cut(s)	7, 960
SmiMI	CAYNNNNRTG	2 cut(s)	184, 274
SmlI	CTYRAG	1 cut(s)	736
SmoI	CTYRAG	1 cut(s)	736
SphI	GCATGC	1 cut(s)	333
Sse9I	AATT	6 cut(s)	378, 408, 458, 549, 556, 708
SsiI	CCGC	5 cut(s)	5, 45, 69, 372, 855
SspDI	GGCGCC	1 cut(s)	537
SspMI	CTAG	4 cut(s)	684, 800, 1067, 1114
StyD4I	CCNGG	4 cut(s)	76, 196, 227, 285
StyI	CCWWGG	5 cut(s)	17, 467, 476, 531, 591
TaaI	ACNGT	3 cut(s)	454, 600, 1002
TaiI	ACGT	1 cut(s)	53
TaqI	TCGA	3 cut(s)	123, 785, 814
TasI	AATT	6 cut(s)	378, 408, 458, 549, 556, 708
TauI	GCSGC	1 cut(s)	71
TfiI	GAWTC	1 cut(s)	503
Tru1I	TTAA	4 cut(s)	381, 422, 606, 711
Tru9I	TTAA	4 cut(s)	381, 422, 606, 711
TscAI	CASTG	2 cut(s)	605, 868
TseFI	GTSAC	3 cut(s)	32, 741, 857
TseI	GCWGC	7 cut(s)	54, 108, 333, 356, 866, 984, 987
Tsp45I	GTSAC	3 cut(s)	32, 741, 857
TspDTI	ATGAA	6 cut(s)	168, 219, 258, 569, 611, 990
TspGWI	ACGGA	2 cut(s)	245, 1073
TspRI	CASTG	2 cut(s)	605, 868
VpaK11BI	GGWCC	2 cut(s)	7, 960
VspI	ATTAAT	1 cut(s)	711
XagI	CCTNNNNNAGG	1 cut(s)	765
XapI	RAATTY	2 cut(s)	408, 556
XbaI	TCTAGA	1 cut(s)	799
XceI	RCATGY	1 cut(s)	333
XspI	CTAG	4 cut(s)	684, 800, 1067, 1114

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.