Rh5AG089000

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0008398
Physical Location & Seq
Reverse (-)
7917780 .. 7921207
3428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG089000.1

Sequence Viewer

Length: 888 bp
ATGGCGTCGGACCCAAAACCAAGACGGCTGAAGGGTCACAAGGCCACCGCCACGTGTTGTATCGCCTCAGTTGCCACCCCTGGCCTCGTTGCCACTGCCGCCGAGGATGGTTGTGTTTGCTGGTTTGATATGAGATGCAAAGAAGTGATAGAGACTATGGGGGCGGGAAAGGAAGCTGTTACATCGTTATGCTTCAAGCCAGGGAATGAAAATGTTGTATATGTGTCATCGGGGAAGGAAGTTAAGAGCTTTGATGTGCGTCTGGGGAGTTCTTGCTCGTGGAAGGCGCTGGAGAGTTTTGAGTATAATAAAGAGGAGATAAATCAGATTGCATGCAGCTCTAAGGCTTCTTTTCTTGCTGCTGCCGATGATGGCGGTGATATTAAGATTGTTGACATTCGCCAGCAGCGCATTTATAAAACATTAAGAGCTGATACTGGTCATACAAGTATATGTAGCAGCGTGCAATTTCTCCCTTGGAGACCTTGGGAAGTTATTACTGGAGGTCTCGATTCGAAGCTAGTGATGTGGGACTTTTCCAAGGGGCGCCCAAACAAAATAATAAATTTTGGTTTGTCTGATGTAAATAATGGTGGTGAGCAGTGTCTTAATCCTGCCTTTGTCCATGCAATAGTAGTTCCAGAAATTGATATGTTAGACATATCAGACAAGGTTTGTGTTGTGGCTCGGGGTGATGGTGCAGTTGACGTGATTAATATTGAAGCTGAACTTGATTCTGTAAGGTCGAAAGGTTCTACAAAACCTCGGAAAGGATCTCAATCAAGATCCAAAAACACCGATCTGGAAATCCAAGATCAAAATAGAAGGAATAGATTGCATTTGGATTACTCATCAGGCGGTCATACTGCTGCTGTGTCTTGCGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

295

Amino Acids

32.05

Weight (kDa)

6.93

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 8 - 183 1.6e-17 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 8 - 86 2.5e-07 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 16 - 158 4.7e-12 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 17 - 189 1.1e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 18 - 179 1.7e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 57 - 189 2.2e-13 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 74 - 182 1.3e-06 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 102 - 178 2.8e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 417
AccB1I GGYRCC 1 cut(s) 546
AciI CCGC 5 cut(s) 48, 99, 164, 375, 858
AclWI GGATC 2 cut(s) 780, 781
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 1 cut(s) 50
AcvI CACGTG 1 cut(s) 54
AcyI GRCGYC 2 cut(s) 5, 547
AfiI CCNNNNNNNGG 1 cut(s) 770
AflIII ACRYGT 1 cut(s) 53
AgsI TTSAA 2 cut(s) 196, 722
AjiI CACGTC 1 cut(s) 709
AjnI CCWGG 2 cut(s) 79, 199
AluBI AGCT 6 cut(s) 176, 249, 339, 431, 520, 725
AluI AGCT 6 cut(s) 176, 249, 339, 431, 520, 725
Alw26I GTCTC 3 cut(s) 146, 475, 512
AlwI GGATC 2 cut(s) 780, 781
Ama87I CYCGRG 1 cut(s) 687
AoxI GGCC 2 cut(s) 42, 82
ApeKI GCWGC 6 cut(s) 336, 359, 362, 406, 459, 869
ApoI RAATTY 1 cut(s) 565
AseI ATTAAT 1 cut(s) 714
AspLEI GCGC 3 cut(s) 289, 411, 549
AspS9I GGNCC 1 cut(s) 10
AsuHPI GGTGA 3 cut(s) 389, 608, 704
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 687
AvaII GGWCC 1 cut(s) 10
BanI GGYRCC 1 cut(s) 546
BauI CACGAG 1 cut(s) 277
BbrPI CACGTG 1 cut(s) 54
BbvI GCAGC 6 cut(s) 346, 348, 349, 418, 471, 856
BccI CCATC 3 cut(s) 101, 365, 689
BceAI ACGGC 1 cut(s) 41
BciT130I CCWGG 2 cut(s) 81, 201
BcoDI GTCTC 3 cut(s) 146, 475, 512
BfaI CTAG 1 cut(s) 521
BfoI RGCGCY 2 cut(s) 290, 550
BisI GCNGC 7 cut(s) 99, 337, 360, 363, 407, 460, 870
BlsI GCNGC 7 cut(s) 100, 338, 361, 364, 408, 461, 871
Bme1390I CCNGG 2 cut(s) 81, 201
Bme18I GGWCC 1 cut(s) 10
BmeT110I CYCGRG 1 cut(s) 687
BmgBI CACGTC 1 cut(s) 709
BmgT120I GGNCC 1 cut(s) 10
BmiI GGNNCC 2 cut(s) 12, 548
BmrFI CCNGG 2 cut(s) 81, 201
BmsI GCATC 1 cut(s) 125
BpmI CTGGAG 2 cut(s) 311, 522
Bpu14I TTCGAA 1 cut(s) 515
BsaAI YACGTR 1 cut(s) 54
BsaBI GATNNNNATC 1 cut(s) 778
BsaHI GRCGYC 2 cut(s) 5, 547
BsaI GGTCTC 2 cut(s) 475, 512
BsaJI CCNNGG 7 cut(s) 79, 102, 200, 476, 485, 540, 764
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse1I ACTGG 2 cut(s) 442, 505
Bse8I GATNNNNATC 1 cut(s) 778
BseBI CCWGG 2 cut(s) 81, 201
BseDI CCNNGG 7 cut(s) 79, 102, 200, 476, 485, 540, 764
BseGI GGATG 1 cut(s) 112
BseJI GATNNNNATC 1 cut(s) 778
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 2 cut(s) 442, 505
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 6 cut(s) 346, 348, 349, 418, 471, 856
BsgI GTGCAG 1 cut(s) 720
BshFI GGCC 2 cut(s) 44, 84
BshNI GGYRCC 1 cut(s) 546
BsiHKCI CYCGRG 1 cut(s) 687
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 1 cut(s) 770
BsmAI GTCTC 3 cut(s) 146, 475, 512
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 2 cut(s) 44, 84
Bso31I GGTCTC 2 cut(s) 475, 512
BsoBI CYCGRG 1 cut(s) 687
Bsp119I TTCGAA 1 cut(s) 515
Bsp143I GATC 4 cut(s) 773, 785, 799, 814
BspACI CCGC 5 cut(s) 48, 99, 164, 375, 858
BspANI GGCC 2 cut(s) 44, 84
BspCNI CTCAG 1 cut(s) 80
BspLI GGNNCC 2 cut(s) 12, 548
BspPI GGATC 2 cut(s) 780, 781
BspT104I TTCGAA 1 cut(s) 515
BspT107I GGYRCC 1 cut(s) 546
BspTNI GGTCTC 2 cut(s) 475, 512
BsrI ACTGG 2 cut(s) 442, 505
BssECI CCNNGG 7 cut(s) 79, 102, 200, 476, 485, 540, 764
BssMI GATC 4 cut(s) 773, 785, 799, 814
BssNI GRCGYC 2 cut(s) 5, 547
BssSI CACGAG 1 cut(s) 277
BssT1I CCWWGG 3 cut(s) 476, 485, 540
Bst2BI CACGAG 1 cut(s) 277
Bst2UI CCWGG 2 cut(s) 81, 201
BstACI GRCGYC 2 cut(s) 5, 547
BstBAI YACGTR 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 3 cut(s) 334, 404, 464
BstDEI CTNAG 2 cut(s) 67, 342
BstENI CCTNNNNNAGG 1 cut(s) 768
BstF5I GGATG 1 cut(s) 112
BstH2I RGCGCY 2 cut(s) 290, 550
BstHHI GCGC 3 cut(s) 289, 411, 549
BstKTI GATC 4 cut(s) 776, 788, 802, 817
BstMAI GTCTC 3 cut(s) 146, 475, 512
BstMBI GATC 4 cut(s) 773, 785, 799, 814
BstMWI GCNNNNNNNGC 3 cut(s) 71, 98, 408
BstNI CCWGG 2 cut(s) 81, 201
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 2 cut(s) 79, 199
BstV1I GCAGC 6 cut(s) 346, 348, 349, 418, 471, 856
BstX2I RGATCY 2 cut(s) 773, 785
BstYI RGATCY 2 cut(s) 773, 785
BsuRI GGCC 2 cut(s) 44, 84
BtrI CACGTC 1 cut(s) 709
BtsCI GGATG 1 cut(s) 112
BtsI GCAGTG 2 cut(s) 93, 608
BtsIMutI CAGTG 2 cut(s) 93, 608
Cac8I GCNNGC 3 cut(s) 334, 404, 464
CfoI GCGC 3 cut(s) 289, 411, 549
Cfr13I GGNCC 1 cut(s) 10
CseI GACGC 1 cut(s) 248
CviAII CATG 2 cut(s) 333, 626
DdeI CTNAG 2 cut(s) 67, 342
DinI GGCGCC 1 cut(s) 548
DpnI GATC 4 cut(s) 775, 787, 801, 816
DpnII GATC 4 cut(s) 773, 785, 799, 814
Eco130I CCWWGG 3 cut(s) 476, 485, 540
Eco31I GGTCTC 2 cut(s) 475, 512
Eco47I GGWCC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 50
Eco72I CACGTG 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 687
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoRII CCWGG 2 cut(s) 79, 199
EcoT14I CCWWGG 3 cut(s) 476, 485, 540
EgeI GGCGCC 1 cut(s) 548
EheI GGCGCC 1 cut(s) 548
ErhI CCWWGG 3 cut(s) 476, 485, 540
FaeI CATG 2 cut(s) 336, 629
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 1 cut(s) 545
FatI CATG 2 cut(s) 332, 625
FauI CCCGC 1 cut(s) 157
Fnu4HI GCNGC 7 cut(s) 99, 337, 360, 363, 407, 460, 870
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 7 cut(s) 99, 337, 360, 363, 407, 460, 870
FspBI CTAG 1 cut(s) 521
GlaI GCGC 3 cut(s) 288, 410, 548
GluI GCNGC 7 cut(s) 99, 337, 360, 363, 407, 460, 870
GsuI CTGGAG 2 cut(s) 311, 522
HaeII RGCGCY 2 cut(s) 290, 550
HaeIII GGCC 2 cut(s) 44, 84
HgaI GACGC 1 cut(s) 248
HhaI GCGC 3 cut(s) 289, 411, 549
Hin1I GRCGYC 2 cut(s) 5, 547
Hin1II CATG 2 cut(s) 336, 629
Hin6I GCGC 3 cut(s) 287, 409, 547
HinP1I GCGC 3 cut(s) 287, 409, 547
HincII GTYRAC 2 cut(s) 394, 706
HindII GTYRAC 2 cut(s) 394, 706
HinfI GANTC 2 cut(s) 512, 734
HphI GGTGA 3 cut(s) 389, 608, 704
Hpy166II GTNNAC 2 cut(s) 394, 706
Hpy188I TCNGA 5 cut(s) 10, 327, 580, 667, 768
Hpy188III TCNNGA 4 cut(s) 509, 641, 783, 803
Hpy8I GTNNAC 2 cut(s) 394, 706
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 25, 229, 277, 819
HpyCH4IV ACGT 2 cut(s) 53, 708
HpyCH4V TGCA 7 cut(s) 138, 332, 336, 466, 629, 701, 838
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 98, 408
HpyF3I CTNAG 2 cut(s) 67, 342
HpySE526I ACGT 2 cut(s) 53, 708
Hsp92I GRCGYC 2 cut(s) 5, 547
Hsp92II CATG 2 cut(s) 336, 629
HspAI GCGC 3 cut(s) 287, 409, 547
KasI GGCGCC 1 cut(s) 546
Kzo9I GATC 4 cut(s) 773, 785, 799, 814
Lsp1109I GCAGC 6 cut(s) 346, 348, 349, 418, 471, 856
LweI GCATC 1 cut(s) 125
MaeI CTAG 1 cut(s) 521
MaeII ACGT 2 cut(s) 53, 708
MaeIII GTNAC 2 cut(s) 35, 178
MalI GATC 4 cut(s) 775, 787, 801, 816
MboI GATC 4 cut(s) 773, 785, 799, 814
MflI RGATCY 2 cut(s) 773, 785
MluCI AATT 3 cut(s) 467, 565, 645
Mly113I GGCGCC 1 cut(s) 547
MnlI CCTC 6 cut(s) 76, 95, 97, 307, 497, 774
MseI TTAA 5 cut(s) 243, 384, 425, 609, 714
MslI CAYNNNNRTG 1 cut(s) 187
MspR9I CCNGG 2 cut(s) 81, 201
MvaI CCWGG 2 cut(s) 81, 201
MwoI GCNNNNNNNGC 3 cut(s) 71, 98, 408
NarI GGCGCC 1 cut(s) 547
NdeII GATC 4 cut(s) 773, 785, 799, 814
NlaIII CATG 2 cut(s) 336, 629
NlaIV GGNNCC 2 cut(s) 12, 548
NmeAIII GCCGAG 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 35
NspI RCATGY 1 cut(s) 336
NspV TTCGAA 1 cut(s) 515
PaeI GCATGC 1 cut(s) 336
PfeI GAWTC 2 cut(s) 512, 734
PkrI GCNGC 7 cut(s) 100, 338, 361, 364, 408, 461, 871
PluTI GGCGCC 1 cut(s) 550
PmaCI CACGTG 1 cut(s) 54
PmlI CACGTG 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 54
PshBI ATTAAT 1 cut(s) 714
PsiI TTATAA 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 79, 199
PspCI CACGTG 1 cut(s) 54
PspGI CCWGG 2 cut(s) 79, 199
PspN4I GGNNCC 2 cut(s) 12, 548
PspPI GGNCC 1 cut(s) 10
PsuI RGATCY 2 cut(s) 773, 785
RseI CAYNNNNRTG 1 cut(s) 187
SaqAI TTAA 5 cut(s) 243, 384, 425, 609, 714
SatI GCNGC 7 cut(s) 99, 337, 360, 363, 407, 460, 870
Sau3AI GATC 4 cut(s) 773, 785, 799, 814
Sau96I GGNCC 1 cut(s) 10
ScrFI CCNGG 2 cut(s) 81, 201
SfaNI GCATC 1 cut(s) 125
SfoI GGCGCC 1 cut(s) 548
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 1 cut(s) 10
SmiMI CAYNNNNRTG 1 cut(s) 187
SphI GCATGC 1 cut(s) 336
Sse9I AATT 3 cut(s) 467, 565, 645
SsiI CCGC 5 cut(s) 48, 99, 164, 375, 858
SspDI GGCGCC 1 cut(s) 546
SspI AATATT 1 cut(s) 718
SspMI CTAG 1 cut(s) 521
StyD4I CCNGG 2 cut(s) 79, 199
StyI CCWWGG 3 cut(s) 476, 485, 540
TaiI ACGT 2 cut(s) 56, 711
TaqI TCGA 3 cut(s) 510, 515, 746
TasI AATT 3 cut(s) 467, 565, 645
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 2 cut(s) 512, 734
Tru1I TTAA 5 cut(s) 243, 384, 425, 609, 714
Tru9I TTAA 5 cut(s) 243, 384, 425, 609, 714
TscAI CASTG 2 cut(s) 100, 608
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 6 cut(s) 336, 359, 362, 406, 459, 869
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 1 cut(s) 222
TspRI CASTG 2 cut(s) 100, 608
VpaK11BI GGWCC 1 cut(s) 10
VspI ATTAAT 1 cut(s) 714
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 1 cut(s) 565
XceI RCATGY 1 cut(s) 336
XspI CTAG 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.