Rh5DG084200

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008398
Physical Location & Seq
Reverse (-)
7361043 .. 7364396
3354 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG084200.1

Sequence Viewer

Length: 999 bp
ATGAGATGCAAAGAAGTGATAGAGACTATGGAGGCGGGAAAGGAAGCTGTTACATCGTTATGCTTCAAGCCAGGGAATGAAAATGTTGTATATGTGTCATCGGGGAAGGAAGTTAAGAGCTTTGATGTGCGTCTGGGGAGTTCTTGCTCGTGGAAGGCGCTGGAGAGTTTTGAGTATAATAAAGAGGAGATAAATCAGATTGCATGCAGCTCTAAGGCTTCTTTTCTTGCTGCTGCCGATGATGGCGGTGATATTAAGATTGTTGACATTCGCCAGCAGCGCATTTATAAAACATTAAGAGCTGATACTGGTCATACAAGTATATGTAGCAGCGTGCAATTTCTCCCTTGGAGACCTTGGGAAGTTATTACTGGAGGTCTCGATTCGAAGCTAGTGATGTGGGACTTTTCCAAGGGGCGCCCAAACAAAATAATAAATTTTGGTTTGTCTGATGTAAATAATGGTGGTGAGCAGTGTCTTAATCCTGCCTTTGTCCATGCAATAGCAGTTCCAGAAATTGATATGTTAGACATATCAGACAAGGTTTGTGTTGTGGCTCGGGGTGATGGTGCAGTTGACGTGATTAATATTGAAGCTGAACTTGATTCTGTAAGTTCGAAAGGTTCTACAAAACCTCGGAAAGGATCTCAATCAAGATCCAAAAACACCGATCTGGAAATCCAAGATCAAAATAGAAGGAATAGATTGCATTTGGATTACTCATCAGGCGGTCATACTGCTGCTGTGTCTTGTGTGGCATTTTCGTTATTTGGGGAAAGAGGAAGATTTATAGTTTCAGGCGGAAACGACAAGCTGGTAAAGGTCTGGGATTGGACCAGGTATCTTGATGCAGAACAAACTAGCGGCAGTGGTGATCTTCTACATTTGAATATCGACCACAGCAAAAAAGTCAACTGGCTGTGTACCACTCCAGGTGATGTGGAAAACCTAGTTGTTTGTGATACAACTAAGGTGGTAAAGGTCTACTCTGTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

332

Amino Acids

36.48

Weight (kDa)

5.93

Isoelectric Point (pI)

29.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 2 - 140 5.2e-09 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 3 - 140 1.8e-10 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 4 - 146 1.1e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 10 - 146 1.1e-13 WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 11 - 140 4.3e-06 WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st PF24817 27 - 154 4.8e-06 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 59 - 135 3.6e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 244 - 277 8.6e-07 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 245 - 331 1.6e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AccB1I GGYRCC 1 cut(s) 417
AccI GTMKAC 1 cut(s) 984
AciI CCGC 5 cut(s) 35, 246, 729, 801, 864
AclWI GGATC 2 cut(s) 651, 652
AcsI RAATTY 1 cut(s) 436
AcyI GRCGYC 1 cut(s) 418
AfaI GTAC 1 cut(s) 925
AfiI CCNNNNNNNGG 1 cut(s) 641
AgsI TTSAA 3 cut(s) 67, 593, 889
AjiI CACGTC 1 cut(s) 580
AjnI CCWGG 3 cut(s) 70, 836, 931
AluBI AGCT 7 cut(s) 47, 120, 210, 302, 391, 596, 814
AluI AGCT 7 cut(s) 47, 120, 210, 302, 391, 596, 814
Alw26I GTCTC 3 cut(s) 17, 346, 383
AlwI GGATC 2 cut(s) 651, 652
Ama87I CYCGRG 1 cut(s) 558
ApeKI GCWGC 6 cut(s) 207, 230, 233, 277, 330, 740
ApoI RAATTY 1 cut(s) 436
AseI ATTAAT 1 cut(s) 585
AspLEI GCGC 3 cut(s) 160, 282, 420
AspS9I GGNCC 1 cut(s) 834
AsuHPI GGTGA 5 cut(s) 260, 479, 575, 884, 947
AsuII TTCGAA 2 cut(s) 386, 617
AvaI CYCGRG 1 cut(s) 558
AvaII GGWCC 1 cut(s) 834
BanI GGYRCC 1 cut(s) 417
BauI CACGAG 1 cut(s) 148
BbvI GCAGC 6 cut(s) 217, 219, 220, 289, 342, 727
BccI CCATC 2 cut(s) 236, 560
BciT130I CCWGG 3 cut(s) 72, 838, 933
BcoDI GTCTC 3 cut(s) 17, 346, 383
BfaI CTAG 3 cut(s) 392, 861, 950
BfoI RGCGCY 2 cut(s) 161, 421
BisI GCNGC 7 cut(s) 208, 231, 234, 278, 331, 741, 865
BlsI GCNGC 7 cut(s) 209, 232, 235, 279, 332, 742, 866
Bme1390I CCNGG 3 cut(s) 72, 838, 933
Bme18I GGWCC 1 cut(s) 834
BmeT110I CYCGRG 1 cut(s) 558
BmgBI CACGTC 1 cut(s) 580
BmgT120I GGNCC 1 cut(s) 834
BmiI GGNNCC 1 cut(s) 419
BmrFI CCNGG 3 cut(s) 72, 838, 933
BmsI GCATC 1 cut(s) 838
BpmI CTGGAG 3 cut(s) 182, 393, 915
Bpu14I TTCGAA 2 cut(s) 386, 617
BsaBI GATNNNNATC 1 cut(s) 649
BsaHI GRCGYC 1 cut(s) 418
BsaI GGTCTC 2 cut(s) 346, 383
BsaJI CCNNGG 5 cut(s) 71, 347, 356, 411, 635
Bsc4I CCNNNNNNNGG 1 cut(s) 641
Bse1I ACTGG 3 cut(s) 313, 376, 920
Bse8I GATNNNNATC 1 cut(s) 649
BseBI CCWGG 3 cut(s) 72, 838, 933
BseDI CCNNGG 5 cut(s) 71, 347, 356, 411, 635
BseJI GATNNNNATC 1 cut(s) 649
BseLI CCNNNNNNNGG 1 cut(s) 641
BseNI ACTGG 3 cut(s) 313, 376, 920
BseRI GAGGAG 1 cut(s) 200
BseXI GCAGC 6 cut(s) 217, 219, 220, 289, 342, 727
BsgI GTGCAG 1 cut(s) 591
BshNI GGYRCC 1 cut(s) 417
BsiHKCI CYCGRG 1 cut(s) 558
BslFI GGGAC 1 cut(s) 416
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 3 cut(s) 17, 346, 383
BsmFI GGGAC 1 cut(s) 416
Bso31I GGTCTC 2 cut(s) 346, 383
BsoBI CYCGRG 1 cut(s) 558
Bsp119I TTCGAA 2 cut(s) 386, 617
Bsp143I GATC 5 cut(s) 644, 656, 670, 685, 874
BspACI CCGC 5 cut(s) 35, 246, 729, 801, 864
BspLI GGNNCC 1 cut(s) 419
BspPI GGATC 2 cut(s) 651, 652
BspT104I TTCGAA 2 cut(s) 386, 617
BspT107I GGYRCC 1 cut(s) 417
BspTNI GGTCTC 2 cut(s) 346, 383
BsrI ACTGG 3 cut(s) 313, 376, 920
BssECI CCNNGG 5 cut(s) 71, 347, 356, 411, 635
BssMI GATC 5 cut(s) 644, 656, 670, 685, 874
BssNI GRCGYC 1 cut(s) 418
BssSI CACGAG 1 cut(s) 148
BssT1I CCWWGG 3 cut(s) 347, 356, 411
Bst2BI CACGAG 1 cut(s) 148
Bst2UI CCWGG 3 cut(s) 72, 838, 933
BstACI GRCGYC 1 cut(s) 418
BstBI TTCGAA 2 cut(s) 386, 617
BstC8I GCNNGC 3 cut(s) 205, 275, 335
BstDEI CTNAG 2 cut(s) 213, 969
BstENI CCTNNNNNAGG 1 cut(s) 639
BstH2I RGCGCY 2 cut(s) 161, 421
BstHHI GCGC 3 cut(s) 160, 282, 420
BstKTI GATC 5 cut(s) 647, 659, 673, 688, 877
BstMAI GTCTC 3 cut(s) 17, 346, 383
BstMBI GATC 5 cut(s) 644, 656, 670, 685, 874
BstMWI GCNNNNNNNGC 1 cut(s) 279
BstNI CCWGG 3 cut(s) 72, 838, 933
BstNSI RCATGY 1 cut(s) 207
BstSCI CCNGG 3 cut(s) 70, 836, 931
BstV1I GCAGC 6 cut(s) 217, 219, 220, 289, 342, 727
BstX2I RGATCY 2 cut(s) 644, 656
BstYI RGATCY 2 cut(s) 644, 656
BtrI CACGTC 1 cut(s) 580
BtsI GCAGTG 2 cut(s) 479, 874
BtsIMutI CAGTG 2 cut(s) 479, 874
Cac8I GCNNGC 3 cut(s) 205, 275, 335
CfoI GCGC 3 cut(s) 160, 282, 420
Cfr13I GGNCC 1 cut(s) 834
CseI GACGC 1 cut(s) 119
CsiI ACCWGGT 1 cut(s) 836
Csp6I GTAC 1 cut(s) 924
CspCI CAANNNNNGTGG 2 cut(s) 954, 989
CviAII CATG 2 cut(s) 204, 497
CviQI GTAC 1 cut(s) 924
DdeI CTNAG 2 cut(s) 213, 969
DinI GGCGCC 1 cut(s) 419
DpnI GATC 5 cut(s) 646, 658, 672, 687, 876
DpnII GATC 5 cut(s) 644, 656, 670, 685, 874
EciI GGCGGA 1 cut(s) 816
Eco130I CCWWGG 3 cut(s) 347, 356, 411
Eco31I GGTCTC 2 cut(s) 346, 383
Eco47I GGWCC 1 cut(s) 834
Eco88I CYCGRG 1 cut(s) 558
EcoNI CCTNNNNNAGG 1 cut(s) 639
EcoRII CCWGG 3 cut(s) 70, 836, 931
EcoT14I CCWWGG 3 cut(s) 347, 356, 411
EgeI GGCGCC 1 cut(s) 419
EheI GGCGCC 1 cut(s) 419
ErhI CCWWGG 3 cut(s) 347, 356, 411
FaeI CATG 2 cut(s) 207, 500
FalI AAGNNNNNCTT 2 cut(s) 585, 617
FaqI GGGAC 1 cut(s) 416
FatI CATG 2 cut(s) 203, 496
FauI CCCGC 1 cut(s) 28
FblI GTMKAC 1 cut(s) 984
Fnu4HI GCNGC 7 cut(s) 208, 231, 234, 278, 331, 741, 865
Fsp4HI GCNGC 7 cut(s) 208, 231, 234, 278, 331, 741, 865
FspBI CTAG 3 cut(s) 392, 861, 950
GlaI GCGC 3 cut(s) 159, 281, 419
GluI GCNGC 7 cut(s) 208, 231, 234, 278, 331, 741, 865
GsuI CTGGAG 3 cut(s) 182, 393, 915
HaeII RGCGCY 2 cut(s) 161, 421
HgaI GACGC 1 cut(s) 119
HhaI GCGC 3 cut(s) 160, 282, 420
Hin1I GRCGYC 1 cut(s) 418
Hin1II CATG 2 cut(s) 207, 500
Hin6I GCGC 3 cut(s) 158, 280, 418
HinP1I GCGC 3 cut(s) 158, 280, 418
HincII GTYRAC 3 cut(s) 265, 577, 913
HindII GTYRAC 3 cut(s) 265, 577, 913
HinfI GANTC 2 cut(s) 383, 605
HphI GGTGA 5 cut(s) 260, 479, 575, 884, 947
Hpy166II GTNNAC 5 cut(s) 265, 577, 913, 924, 985
Hpy188I TCNGA 4 cut(s) 198, 451, 538, 639
Hpy188III TCNNGA 5 cut(s) 380, 512, 654, 674, 845
Hpy8I GTNNAC 5 cut(s) 265, 577, 913, 924, 985
HpyAV CCTTC 3 cut(s) 100, 148, 690
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 9 cut(s) 9, 203, 207, 337, 500, 572, 709, 851, 995
HpyF10VI GCNNNNNNNGC 1 cut(s) 279
HpyF3I CTNAG 2 cut(s) 213, 969
HpySE526I ACGT 1 cut(s) 579
Hsp92I GRCGYC 1 cut(s) 418
Hsp92II CATG 2 cut(s) 207, 500
HspAI GCGC 3 cut(s) 158, 280, 418
KasI GGCGCC 1 cut(s) 417
Kzo9I GATC 5 cut(s) 644, 656, 670, 685, 874
Lsp1109I GCAGC 6 cut(s) 217, 219, 220, 289, 342, 727
LweI GCATC 1 cut(s) 838
MabI ACCWGGT 1 cut(s) 836
MaeI CTAG 3 cut(s) 392, 861, 950
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 1 cut(s) 49
MalI GATC 5 cut(s) 646, 658, 672, 687, 876
MboI GATC 5 cut(s) 644, 656, 670, 685, 874
MboII GAAGA 2 cut(s) 795, 869
MflI RGATCY 2 cut(s) 644, 656
MluCI AATT 3 cut(s) 338, 436, 516
Mly113I GGCGCC 1 cut(s) 418
MnlI CCTC 5 cut(s) 25, 178, 368, 645, 773
MseI TTAA 5 cut(s) 114, 255, 296, 480, 585
MslI CAYNNNNRTG 1 cut(s) 58
MspR9I CCNGG 3 cut(s) 72, 838, 933
MvaI CCWGG 3 cut(s) 72, 838, 933
MwoI GCNNNNNNNGC 1 cut(s) 279
NarI GGCGCC 1 cut(s) 418
NdeII GATC 5 cut(s) 644, 656, 670, 685, 874
NlaIII CATG 2 cut(s) 207, 500
NlaIV GGNNCC 1 cut(s) 419
NspI RCATGY 1 cut(s) 207
NspV TTCGAA 2 cut(s) 386, 617
PaeI GCATGC 1 cut(s) 207
PfeI GAWTC 2 cut(s) 383, 605
PkrI GCNGC 7 cut(s) 209, 232, 235, 279, 332, 742, 866
PluTI GGCGCC 1 cut(s) 421
PshBI ATTAAT 1 cut(s) 585
PsiI TTATAA 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 70, 836, 931
PspGI CCWGG 3 cut(s) 70, 836, 931
PspN4I GGNNCC 1 cut(s) 419
PspPI GGNCC 1 cut(s) 834
PsuI RGATCY 2 cut(s) 644, 656
RsaI GTAC 1 cut(s) 925
RsaNI GTAC 1 cut(s) 924
RseI CAYNNNNRTG 1 cut(s) 58
SaqAI TTAA 5 cut(s) 114, 255, 296, 480, 585
SatI GCNGC 7 cut(s) 208, 231, 234, 278, 331, 741, 865
Sau3AI GATC 5 cut(s) 644, 656, 670, 685, 874
Sau96I GGNCC 1 cut(s) 834
ScrFI CCNGG 3 cut(s) 72, 838, 933
SexAI ACCWGGT 1 cut(s) 836
SfaNI GCATC 1 cut(s) 838
SfoI GGCGCC 1 cut(s) 419
SfuI TTCGAA 2 cut(s) 386, 617
SinI GGWCC 1 cut(s) 834
SmiMI CAYNNNNRTG 1 cut(s) 58
SphI GCATGC 1 cut(s) 207
Sse9I AATT 3 cut(s) 338, 436, 516
SsiI CCGC 5 cut(s) 35, 246, 729, 801, 864
SspDI GGCGCC 1 cut(s) 417
SspI AATATT 1 cut(s) 589
SspMI CTAG 3 cut(s) 392, 861, 950
StyD4I CCNGG 3 cut(s) 70, 836, 931
StyI CCWWGG 3 cut(s) 347, 356, 411
TaiI ACGT 1 cut(s) 582
TaqI TCGA 4 cut(s) 381, 386, 617, 894
TasI AATT 3 cut(s) 338, 436, 516
TauI GCSGC 1 cut(s) 867
TfiI GAWTC 2 cut(s) 383, 605
Tru1I TTAA 5 cut(s) 114, 255, 296, 480, 585
Tru9I TTAA 5 cut(s) 114, 255, 296, 480, 585
TscAI CASTG 2 cut(s) 479, 874
TseI GCWGC 6 cut(s) 207, 230, 233, 277, 330, 740
TspDTI ATGAA 1 cut(s) 93
TspRI CASTG 2 cut(s) 479, 874
VpaK11BI GGWCC 1 cut(s) 834
VspI ATTAAT 1 cut(s) 585
XagI CCTNNNNNAGG 1 cut(s) 639
XapI RAATTY 1 cut(s) 436
XceI RCATGY 1 cut(s) 207
XmiI GTMKAC 1 cut(s) 984
XspI CTAG 3 cut(s) 392, 861, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.