FvH4_1g07520

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008398
Physical Location & Seq
Forward (+)
4003028 .. 4006343
3316 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g07520.t3

Sequence Viewer

Length: 1128 bp
ATGGCGACGGACCCAAAACCAAGACGGCTGAGGGGTCACAACGCCACCGCCACGTGTTGTATCGCCTCAGCTGCCACCCCAAGCCTCGTTGCCACTGCCGCCGAGGATGGTTGTGTTTGCTGGTTTGATATGAGATGCAAAGAAGTGATGGACATCATGGAGGTGGGAAAGGAAGCTGTTACATCGTTATGTTTCAAGCCAGGGAATGAGAATGTTGTATATGTGTCATCGGGAAAGGAAGTCAAGAGCTTTGATGTGCGAATGGGGAGTTCTTGCTCATGGAAGGTGGTGGATTGTTTTGAGTATAATAAAGAGGAGATAAATCAGATTGCATGCAGCTCTAAGGCTTCTTTTCTTGCTGCTGCTGATGATGGCGGTGATATTAAGATTGTTGACATTCGCCAGCAGCGCATTTACAAAACATTAAGAGCTGATACTGGTCATACAAGTATATGTAGCAGTGTGCAATTTCTTCCTTGGAGACCTTGGGAAGTTATTACTGGAGGTCTTGATTCGAAGCTAGTGATGTGGGACTTTTCCAAGGGGCGGCCAAACAAAATAATAAATTTTGGTTTGTCTGACGTAAATAATGGTGGTGAGCAGTGTTTTAATCCTGCCTTTGTCCATGCAATAGCAGTTCCAGAAATTGATATGTTAGGCAAATCAGACAAGGTTTGTGTCGTGGCTCGGGGTGATGGTGCAGTTGACGTGATTAACATTGAAGCTGAACTTGATTCTGTTAGGTCAAAAGGTTCTACAAAACCTCGGAAAGGATCTCAATCAAGATCCAAAGACACCGATTCGGAAATCCAAGATCAAAATGGAAGGAATAGGTTGCATTTGGATTACTCATCAGGTGGTCATACTGCTGCTGTGTCTTGCGTGGCGTTTTCGTTATTTGGGGAAAGAGGAAGATTCATAATTTCCGGTGGAAACGACAAGCTGGTAAAGGTCTGGGATTGGACCAGGGATCTTGATGCAGAACAAACTAGCGGCAGTTGTGATCTTCTACATTTGAATATCAACCACAACAAAAAAGTCAACTGGCTGTGTACCACTCCAGCTGATACAGAAAACCTAGTTGTTTGTGATACAACTAAGGTGGTAAAGGTCTACTCTGTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

376

Amino Acids

41.01

Weight (kDa)

6.2

Isoelectric Point (pI)

31.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 5 - 139 3.5e-08 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 10 - 140 1e-09 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 10 - 146 3e-10 CDC20/Fizzy WD40 domain
Beta-prop_EIPR1 PF23609 10 - 134 9.2e-06 EIPR1 beta-propeller
Beta-prop_WDR5 PF25175 14 - 146 1.6e-13 WDR5 beta-propeller domain
WD40_Prp19 PF24814 14 - 114 1e-05 Prp19 WD40 domain
Beta-prop_EML_2 PF23414 59 - 135 3.9e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 61 - 171 3.2e-06 WDHD1 first WD40 domain
WD40 PF00400 244 - 277 1.3e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 245 - 331 4.5e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1113
AciI CCGC 5 cut(s) 48, 99, 375, 547, 993
AclWI GGATC 3 cut(s) 780, 781, 978
AcoI YGGCCR 1 cut(s) 548
AcsI RAATTY 1 cut(s) 565
AcvI CACGTG 1 cut(s) 54
AfaI GTAC 1 cut(s) 1054
AfiI CCNNNNNNNGG 2 cut(s) 546, 770
AflIII ACRYGT 1 cut(s) 53
AgsI TTSAA 3 cut(s) 196, 722, 1018
AjiI CACGTC 1 cut(s) 709
AjnI CCWGG 2 cut(s) 199, 965
AluBI AGCT 9 cut(s) 71, 176, 249, 339, 431, 520, 725, 943, 1064
AluI AGCT 9 cut(s) 71, 176, 249, 339, 431, 520, 725, 943, 1064
Alw26I GTCTC 1 cut(s) 475
AlwI GGATC 3 cut(s) 780, 781, 978
Ama87I CYCGRG 1 cut(s) 687
AoxI GGCC 1 cut(s) 548
ApeKI GCWGC 6 cut(s) 71, 336, 359, 362, 406, 869
ApoI RAATTY 1 cut(s) 565
AspLEI GCGC 1 cut(s) 411
AspS9I GGNCC 2 cut(s) 10, 963
AsuHPI GGTGA 3 cut(s) 389, 608, 704
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 687
AvaII GGWCC 2 cut(s) 10, 963
BbrPI CACGTG 1 cut(s) 54
BbvCI CCTCAGC 2 cut(s) 29, 67
BbvI GCAGC 6 cut(s) 58, 346, 348, 349, 418, 856
BccI CCATC 4 cut(s) 101, 142, 365, 689
BceAI ACGGC 1 cut(s) 41
BciT130I CCWGG 2 cut(s) 201, 967
BcoDI GTCTC 1 cut(s) 475
BfaI CTAG 3 cut(s) 521, 990, 1079
BisI GCNGC 9 cut(s) 72, 99, 337, 360, 363, 407, 548, 870, 994
BlsI GCNGC 9 cut(s) 73, 100, 338, 361, 364, 408, 549, 871, 995
Bme1390I CCNGG 2 cut(s) 201, 967
Bme18I GGWCC 2 cut(s) 10, 963
BmeT110I CYCGRG 1 cut(s) 687
BmgBI CACGTC 1 cut(s) 709
BmgT120I GGNCC 2 cut(s) 10, 963
BmiI GGNNCC 1 cut(s) 12
BmrFI CCNGG 2 cut(s) 201, 967
BmsI GCATC 2 cut(s) 125, 967
BpmI CTGGAG 2 cut(s) 522, 1044
Bpu10I CCTNAGC 2 cut(s) 29, 67
Bpu14I TTCGAA 1 cut(s) 515
BsaAI YACGTR 1 cut(s) 54
BsaBI GATNNNNATC 2 cut(s) 152, 778
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 7 cut(s) 102, 200, 476, 485, 540, 764, 966
BsaWI WCCGGW 1 cut(s) 926
Bsc4I CCNNNNNNNGG 2 cut(s) 546, 770
Bse1I ACTGG 3 cut(s) 442, 505, 1049
Bse8I GATNNNNATC 2 cut(s) 152, 778
BseBI CCWGG 2 cut(s) 201, 967
BseDI CCNNGG 7 cut(s) 102, 200, 476, 485, 540, 764, 966
BseGI GGATG 1 cut(s) 112
BseJI GATNNNNATC 2 cut(s) 152, 778
BseLI CCNNNNNNNGG 2 cut(s) 546, 770
BseMII CTCAG 2 cut(s) 20, 81
BseNI ACTGG 3 cut(s) 442, 505, 1049
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 6 cut(s) 58, 346, 348, 349, 418, 856
BsgI GTGCAG 1 cut(s) 720
BshFI GGCC 1 cut(s) 550
BsiHKCI CYCGRG 1 cut(s) 687
BsiSI CCGG 1 cut(s) 927
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 2 cut(s) 546, 770
BsmAI GTCTC 1 cut(s) 475
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 1 cut(s) 550
Bso31I GGTCTC 1 cut(s) 475
BsoBI CYCGRG 1 cut(s) 687
Bsp119I TTCGAA 1 cut(s) 515
Bsp143I GATC 5 cut(s) 773, 785, 814, 970, 1003
BspACI CCGC 5 cut(s) 48, 99, 375, 547, 993
BspANI GGCC 1 cut(s) 550
BspCNI CTCAG 2 cut(s) 21, 80
BspLI GGNNCC 1 cut(s) 12
BspPI GGATC 3 cut(s) 780, 781, 978
BspT104I TTCGAA 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 475
BsrI ACTGG 3 cut(s) 442, 505, 1049
BssECI CCNNGG 7 cut(s) 102, 200, 476, 485, 540, 764, 966
BssMI GATC 5 cut(s) 773, 785, 814, 970, 1003
BssT1I CCWWGG 3 cut(s) 476, 485, 540
Bst2UI CCWGG 2 cut(s) 201, 967
BstBAI YACGTR 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 334, 404
BstDEI CTNAG 5 cut(s) 29, 67, 342, 1098, 1125
BstENI CCTNNNNNAGG 1 cut(s) 768
BstF5I GGATG 1 cut(s) 112
BstHHI GCGC 1 cut(s) 411
BstKTI GATC 5 cut(s) 776, 788, 817, 973, 1006
BstMAI GTCTC 1 cut(s) 475
BstMBI GATC 5 cut(s) 773, 785, 814, 970, 1003
BstMWI GCNNNNNNNGC 3 cut(s) 71, 98, 408
BstNI CCWGG 2 cut(s) 201, 967
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 2 cut(s) 199, 965
BstV1I GCAGC 6 cut(s) 58, 346, 348, 349, 418, 856
BstX2I RGATCY 3 cut(s) 773, 785, 970
BstYI RGATCY 3 cut(s) 773, 785, 970
BsuRI GGCC 1 cut(s) 550
BtrI CACGTC 1 cut(s) 709
BtsCI GGATG 1 cut(s) 112
BtsI GCAGTG 3 cut(s) 93, 466, 608
BtsIMutI CAGTG 3 cut(s) 93, 466, 608
Cac8I GCNNGC 2 cut(s) 334, 404
CfoI GCGC 1 cut(s) 411
Cfr13I GGNCC 2 cut(s) 10, 963
Csp6I GTAC 1 cut(s) 1053
CspCI CAANNNNNGTGG 2 cut(s) 1083, 1118
CviAII CATG 4 cut(s) 157, 279, 333, 626
CviQI GTAC 1 cut(s) 1053
DdeI CTNAG 5 cut(s) 29, 67, 342, 1098, 1125
DpnI GATC 5 cut(s) 775, 787, 816, 972, 1005
DpnII GATC 5 cut(s) 773, 785, 814, 970, 1003
EaeI YGGCCR 1 cut(s) 548
Eco130I CCWWGG 3 cut(s) 476, 485, 540
Eco31I GGTCTC 1 cut(s) 475
Eco47I GGWCC 2 cut(s) 10, 963
Eco72I CACGTG 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 687
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoRII CCWGG 2 cut(s) 199, 965
EcoT14I CCWWGG 3 cut(s) 476, 485, 540
ErhI CCWWGG 3 cut(s) 476, 485, 540
FaeI CATG 4 cut(s) 160, 282, 336, 629
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 1 cut(s) 545
FatI CATG 4 cut(s) 156, 278, 332, 625
FblI GTMKAC 1 cut(s) 1113
Fnu4HI GCNGC 9 cut(s) 72, 99, 337, 360, 363, 407, 548, 870, 994
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 9 cut(s) 72, 99, 337, 360, 363, 407, 548, 870, 994
FspBI CTAG 3 cut(s) 521, 990, 1079
GlaI GCGC 1 cut(s) 410
GluI GCNGC 9 cut(s) 72, 99, 337, 360, 363, 407, 548, 870, 994
GsuI CTGGAG 2 cut(s) 522, 1044
HaeIII GGCC 1 cut(s) 550
HapII CCGG 1 cut(s) 927
HhaI GCGC 1 cut(s) 411
Hin1II CATG 4 cut(s) 160, 282, 336, 629
Hin6I GCGC 1 cut(s) 409
HinP1I GCGC 1 cut(s) 409
HincII GTYRAC 3 cut(s) 394, 706, 1042
HindII GTYRAC 3 cut(s) 394, 706, 1042
HinfI GANTC 4 cut(s) 512, 734, 800, 915
HpaII CCGG 1 cut(s) 927
HphI GGTGA 3 cut(s) 389, 608, 704
Hpy166II GTNNAC 5 cut(s) 394, 706, 1042, 1053, 1114
Hpy188I TCNGA 5 cut(s) 327, 580, 667, 768, 805
Hpy188III TCNNGA 6 cut(s) 231, 244, 509, 641, 783, 974
Hpy8I GTNNAC 5 cut(s) 394, 706, 1042, 1053, 1114
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 277, 819
HpyCH4IV ACGT 3 cut(s) 53, 582, 708
HpyCH4V TGCA 8 cut(s) 138, 332, 336, 466, 629, 701, 838, 980
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 98, 408
HpyF3I CTNAG 5 cut(s) 29, 67, 342, 1098, 1125
HpySE526I ACGT 3 cut(s) 53, 582, 708
Hsp92II CATG 4 cut(s) 160, 282, 336, 629
HspAI GCGC 1 cut(s) 409
Kzo9I GATC 5 cut(s) 773, 785, 814, 970, 1003
Lsp1109I GCAGC 6 cut(s) 58, 346, 348, 349, 418, 856
LweI GCATC 2 cut(s) 125, 967
MaeI CTAG 3 cut(s) 521, 990, 1079
MaeII ACGT 3 cut(s) 53, 582, 708
MaeIII GTNAC 2 cut(s) 35, 178
MalI GATC 5 cut(s) 775, 787, 816, 972, 1005
MboI GATC 5 cut(s) 773, 785, 814, 970, 1003
MboII GAAGA 3 cut(s) 464, 924, 998
MflI RGATCY 3 cut(s) 773, 785, 970
MluCI AATT 4 cut(s) 467, 565, 645, 921
MnlI CCTC 9 cut(s) 24, 76, 95, 97, 154, 307, 497, 774, 902
MseI TTAA 4 cut(s) 384, 425, 609, 714
MslI CAYNNNNRTG 2 cut(s) 161, 187
MspA1I CMGCKG 2 cut(s) 71, 1064
MspI CCGG 1 cut(s) 927
MspR9I CCNGG 2 cut(s) 201, 967
MvaI CCWGG 2 cut(s) 201, 967
MwoI GCNNNNNNNGC 3 cut(s) 71, 98, 408
NdeII GATC 5 cut(s) 773, 785, 814, 970, 1003
NlaIII CATG 4 cut(s) 160, 282, 336, 629
NlaIV GGNNCC 1 cut(s) 12
NmeAIII GCCGAG 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 35
NspI RCATGY 1 cut(s) 336
NspV TTCGAA 1 cut(s) 515
PaeI GCATGC 1 cut(s) 336
PfeI GAWTC 4 cut(s) 512, 734, 800, 915
PkrI GCNGC 9 cut(s) 73, 100, 338, 361, 364, 408, 549, 871, 995
PmaCI CACGTG 1 cut(s) 54
PmlI CACGTG 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 54
Psp6I CCWGG 2 cut(s) 199, 965
PspCI CACGTG 1 cut(s) 54
PspGI CCWGG 2 cut(s) 199, 965
PspN4I GGNNCC 1 cut(s) 12
PspPI GGNCC 2 cut(s) 10, 963
PsuI RGATCY 3 cut(s) 773, 785, 970
PvuII CAGCTG 2 cut(s) 71, 1064
RsaI GTAC 1 cut(s) 1054
RsaNI GTAC 1 cut(s) 1053
RseI CAYNNNNRTG 2 cut(s) 161, 187
SaqAI TTAA 4 cut(s) 384, 425, 609, 714
SatI GCNGC 9 cut(s) 72, 99, 337, 360, 363, 407, 548, 870, 994
Sau3AI GATC 5 cut(s) 773, 785, 814, 970, 1003
Sau96I GGNCC 2 cut(s) 10, 963
ScrFI CCNGG 2 cut(s) 201, 967
SfaNI GCATC 2 cut(s) 125, 967
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 2 cut(s) 10, 963
SmiMI CAYNNNNRTG 2 cut(s) 161, 187
SphI GCATGC 1 cut(s) 336
Sse9I AATT 4 cut(s) 467, 565, 645, 921
SsiI CCGC 5 cut(s) 48, 99, 375, 547, 993
SspMI CTAG 3 cut(s) 521, 990, 1079
StyD4I CCNGG 2 cut(s) 199, 965
StyI CCWWGG 3 cut(s) 476, 485, 540
TaiI ACGT 3 cut(s) 56, 585, 711
TaqI TCGA 1 cut(s) 515
TasI AATT 4 cut(s) 467, 565, 645, 921
TauI GCSGC 3 cut(s) 101, 550, 996
TfiI GAWTC 4 cut(s) 512, 734, 800, 915
Tru1I TTAA 4 cut(s) 384, 425, 609, 714
Tru9I TTAA 4 cut(s) 384, 425, 609, 714
TscAI CASTG 3 cut(s) 100, 466, 608
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 6 cut(s) 71, 336, 359, 362, 406, 869
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 1 cut(s) 907
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 3 cut(s) 100, 466, 608
VpaK11BI GGWCC 2 cut(s) 10, 963
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 1 cut(s) 565
XceI RCATGY 1 cut(s) 336
XcmI CCANNNNNNNNNTGG 1 cut(s) 818
XmiI GTMKAC 1 cut(s) 1113
XspI CTAG 3 cut(s) 521, 990, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.