Rh5BG084200

WD domain, G-beta repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008398
Physical Location & Seq
Reverse (-)
7250174 .. 7253008
2835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG084200.1

Sequence Viewer

Length: 1128 bp
ATGGCGACGGACCCAAAACCAAGACGGCTGAAGGGTCACAAGGCCACCGCCACGTGTTGTATCGCCTCAGTGGCCACCCCTGGCCTCGTTGCCACTGCCGCCGAGGATGGTTGTGTTTGCTGGTTTGATATGAGATGCAAAGAAGTGATAGAGACTATGGAGGTGGGAAAGGAAGCTGTTACATCGTTATGCTTCAAGCCAGGGAATGAAAATGTTGTATATTTGTCATCGGGGAAGGAAGTTAAGAGCTTTGATGTGCGTCTGGGGAGTTCTTGCTCGTGGAAGGTGCTGGAGAGTTTTGAGTATAATAAAGAGGAGATAAATCAGATTGCATGCAGCTCTAAGGCTTCTTTTCTTGCTGCTGCCGATGATGGCGGTGATATTAAGATTGTTGACATTCGCCAGCAGCGCATTTACAAAACATTAAGAGCTGATACTGGTCATACAAGTATATGTAGCAGTGTGCAATTTCTCCCTTGGAGACCTTGGGAAGTTATTACTGGAGGTCTCGATTCGAAGCTAGTGATGTGGGACTTTTCCAAGGGGCGCCCAAACAAAATAATAAATTTTGGTTTGTTTGATGTAAATAATGGTGGTGAGCAGTGTCTTAATCCTGCCTTTGTCCATGCAATAGCAGTTCCAGAAATTGATATGTTAGACAAATCAGACAAGGTTTGTGTTGTGGCTCGGGGTGATGGTGCAGTTGATGTGATTAATATCGAAGCTGAACTTGATTCTGTAAGGTCGAAAGGTTCTACAAAACCTCGGAAAGGATCTCAATCAAAATCCAAAAACACCGATCTGGAAATCCAAGATCAAAATAGAAGGAATAGATTGCATTTGGATTACTCATCAGGCGGTCATACTGCTGCTGTGTCTTGCGTGGCATTTTCGTTATTTGGGGAAAGAGGAAGATTTATAGTTTCAGGCGGAAACGACAAGCTGGTAAAGGTCTGGGATTGGACCAGGTATCTTGATGCAGAACAAACTAGCGGCAGTGGTGATCTTCTACATTTGAATATCGACCACAGCAAAAAAGTCAACTGGCTGTGTACCACTCCAGGTGATGTGGAAAACCTAGTTGTTTGTGATACAACTAAGGTGGTAAAGGTCTACTCTGTTGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

375

Amino Acids

41.11

Weight (kDa)

6.66

Isoelectric Point (pI)

28.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 8 - 183 1.6e-17 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 8 - 86 2e-06 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 16 - 158 5.4e-12 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 18 - 188 1.8e-14 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 19 - 179 1.9e-08 WDR36/Utp21 first beta-propeller
Beta-prop_WDR5 PF25175 57 - 188 5.4e-13 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 73 - 182 1.9e-06 WDR3 first beta-propeller domain
Beta-prop_EML_2 PF23414 102 - 178 4.5e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40 PF00400 287 - 320 1e-06 WD domain, G-beta repeat
Beta-prop_THOC3 PF25174 288 - 374 1.9e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 546
AccI GTMKAC 1 cut(s) 1113
AciI CCGC 6 cut(s) 48, 99, 375, 858, 930, 993
AclWI GGATC 1 cut(s) 781
AcoI YGGCCR 1 cut(s) 72
AcsI RAATTY 1 cut(s) 565
AcuI CTGAAG 1 cut(s) 50
AcvI CACGTG 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 547
AfaI GTAC 1 cut(s) 1054
AfiI CCNNNNNNNGG 1 cut(s) 770
AflIII ACRYGT 1 cut(s) 53
AgsI TTSAA 2 cut(s) 196, 1018
AjnI CCWGG 4 cut(s) 79, 199, 965, 1060
AluBI AGCT 7 cut(s) 176, 249, 339, 431, 520, 725, 943
AluI AGCT 7 cut(s) 176, 249, 339, 431, 520, 725, 943
Alw26I GTCTC 3 cut(s) 146, 475, 512
AlwI GGATC 1 cut(s) 781
Ama87I CYCGRG 1 cut(s) 687
AoxI GGCC 3 cut(s) 42, 72, 82
ApeKI GCWGC 5 cut(s) 336, 359, 362, 406, 869
ApoI RAATTY 1 cut(s) 565
AseI ATTAAT 1 cut(s) 714
AspLEI GCGC 2 cut(s) 411, 549
AspS9I GGNCC 2 cut(s) 10, 963
AsuHPI GGTGA 5 cut(s) 389, 608, 704, 1013, 1076
AsuII TTCGAA 1 cut(s) 515
AvaI CYCGRG 1 cut(s) 687
AvaII GGWCC 2 cut(s) 10, 963
BalI TGGCCA 1 cut(s) 74
BanI GGYRCC 1 cut(s) 546
BauI CACGAG 1 cut(s) 277
BbrPI CACGTG 1 cut(s) 54
BbvI GCAGC 5 cut(s) 346, 348, 349, 418, 856
BccI CCATC 3 cut(s) 101, 365, 689
BceAI ACGGC 1 cut(s) 41
BciT130I CCWGG 4 cut(s) 81, 201, 967, 1062
BcoDI GTCTC 3 cut(s) 146, 475, 512
BfaI CTAG 3 cut(s) 521, 990, 1079
BfoI RGCGCY 1 cut(s) 550
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 7 cut(s) 99, 337, 360, 363, 407, 870, 994
BlsI GCNGC 7 cut(s) 100, 338, 361, 364, 408, 871, 995
Bme1390I CCNGG 4 cut(s) 81, 201, 967, 1062
Bme18I GGWCC 2 cut(s) 10, 963
BmeT110I CYCGRG 1 cut(s) 687
BmgT120I GGNCC 2 cut(s) 10, 963
BmiI GGNNCC 2 cut(s) 12, 548
BmrFI CCNGG 4 cut(s) 81, 201, 967, 1062
BmsI GCATC 2 cut(s) 125, 967
BpmI CTGGAG 3 cut(s) 311, 522, 1044
Bpu14I TTCGAA 1 cut(s) 515
BsaAI YACGTR 1 cut(s) 54
BsaBI GATNNNNATC 2 cut(s) 716, 778
BsaHI GRCGYC 1 cut(s) 547
BsaI GGTCTC 2 cut(s) 475, 512
BsaJI CCNNGG 7 cut(s) 79, 102, 200, 476, 485, 540, 764
Bsc4I CCNNNNNNNGG 1 cut(s) 770
Bse1I ACTGG 3 cut(s) 442, 505, 1049
Bse8I GATNNNNATC 2 cut(s) 716, 778
BseBI CCWGG 4 cut(s) 81, 201, 967, 1062
BseDI CCNNGG 7 cut(s) 79, 102, 200, 476, 485, 540, 764
BseGI GGATG 1 cut(s) 112
BseJI GATNNNNATC 2 cut(s) 716, 778
BseLI CCNNNNNNNGG 1 cut(s) 770
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 3 cut(s) 442, 505, 1049
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 5 cut(s) 346, 348, 349, 418, 856
BsgI GTGCAG 1 cut(s) 720
BshFI GGCC 3 cut(s) 44, 74, 84
BshNI GGYRCC 1 cut(s) 546
BsiHKCI CYCGRG 1 cut(s) 687
BslFI GGGAC 1 cut(s) 545
BslI CCNNNNNNNGG 1 cut(s) 770
BsmAI GTCTC 3 cut(s) 146, 475, 512
BsmFI GGGAC 1 cut(s) 545
BsnI GGCC 3 cut(s) 44, 74, 84
Bso31I GGTCTC 2 cut(s) 475, 512
BsoBI CYCGRG 1 cut(s) 687
Bsp119I TTCGAA 1 cut(s) 515
Bsp143I GATC 4 cut(s) 773, 799, 814, 1003
BspACI CCGC 6 cut(s) 48, 99, 375, 858, 930, 993
BspANI GGCC 3 cut(s) 44, 74, 84
BspCNI CTCAG 1 cut(s) 80
BspLI GGNNCC 2 cut(s) 12, 548
BspPI GGATC 1 cut(s) 781
BspT104I TTCGAA 1 cut(s) 515
BspT107I GGYRCC 1 cut(s) 546
BspTNI GGTCTC 2 cut(s) 475, 512
BsrI ACTGG 3 cut(s) 442, 505, 1049
BssECI CCNNGG 7 cut(s) 79, 102, 200, 476, 485, 540, 764
BssMI GATC 4 cut(s) 773, 799, 814, 1003
BssNI GRCGYC 1 cut(s) 547
BssSI CACGAG 1 cut(s) 277
BssT1I CCWWGG 3 cut(s) 476, 485, 540
Bst2BI CACGAG 1 cut(s) 277
Bst2UI CCWGG 4 cut(s) 81, 201, 967, 1062
BstACI GRCGYC 1 cut(s) 547
BstBAI YACGTR 1 cut(s) 54
BstBI TTCGAA 1 cut(s) 515
BstC8I GCNNGC 2 cut(s) 334, 404
BstDEI CTNAG 3 cut(s) 67, 342, 1098
BstENI CCTNNNNNAGG 1 cut(s) 768
BstF5I GGATG 1 cut(s) 112
BstH2I RGCGCY 1 cut(s) 550
BstHHI GCGC 2 cut(s) 411, 549
BstKTI GATC 4 cut(s) 776, 802, 817, 1006
BstMAI GTCTC 3 cut(s) 146, 475, 512
BstMBI GATC 4 cut(s) 773, 799, 814, 1003
BstMWI GCNNNNNNNGC 3 cut(s) 71, 98, 408
BstNI CCWGG 4 cut(s) 81, 201, 967, 1062
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 4 cut(s) 79, 199, 965, 1060
BstV1I GCAGC 5 cut(s) 346, 348, 349, 418, 856
BstX2I RGATCY 1 cut(s) 773
BstYI RGATCY 1 cut(s) 773
BsuRI GGCC 3 cut(s) 44, 74, 84
BtsCI GGATG 1 cut(s) 112
BtsI GCAGTG 4 cut(s) 93, 466, 608, 1003
BtsIMutI CAGTG 5 cut(s) 75, 93, 466, 608, 1003
Cac8I GCNNGC 2 cut(s) 334, 404
CfoI GCGC 2 cut(s) 411, 549
Cfr13I GGNCC 2 cut(s) 10, 963
CseI GACGC 1 cut(s) 248
CsiI ACCWGGT 1 cut(s) 965
Csp6I GTAC 1 cut(s) 1053
CspCI CAANNNNNGTGG 2 cut(s) 1083, 1118
CviAII CATG 2 cut(s) 333, 626
CviQI GTAC 1 cut(s) 1053
DdeI CTNAG 3 cut(s) 67, 342, 1098
DinI GGCGCC 1 cut(s) 548
DpnI GATC 4 cut(s) 775, 801, 816, 1005
DpnII GATC 4 cut(s) 773, 799, 814, 1003
EaeI YGGCCR 1 cut(s) 72
EciI GGCGGA 1 cut(s) 945
Eco130I CCWWGG 3 cut(s) 476, 485, 540
Eco31I GGTCTC 2 cut(s) 475, 512
Eco47I GGWCC 2 cut(s) 10, 963
Eco57I CTGAAG 1 cut(s) 50
Eco72I CACGTG 1 cut(s) 54
Eco88I CYCGRG 1 cut(s) 687
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoRII CCWGG 4 cut(s) 79, 199, 965, 1060
EcoT14I CCWWGG 3 cut(s) 476, 485, 540
EgeI GGCGCC 1 cut(s) 548
EheI GGCGCC 1 cut(s) 548
ErhI CCWWGG 3 cut(s) 476, 485, 540
FaeI CATG 2 cut(s) 336, 629
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 1 cut(s) 545
FatI CATG 2 cut(s) 332, 625
FblI GTMKAC 1 cut(s) 1113
Fnu4HI GCNGC 7 cut(s) 99, 337, 360, 363, 407, 870, 994
FokI GGATG 1 cut(s) 119
Fsp4HI GCNGC 7 cut(s) 99, 337, 360, 363, 407, 870, 994
FspBI CTAG 3 cut(s) 521, 990, 1079
GlaI GCGC 2 cut(s) 410, 548
GluI GCNGC 7 cut(s) 99, 337, 360, 363, 407, 870, 994
GsuI CTGGAG 3 cut(s) 311, 522, 1044
HaeII RGCGCY 1 cut(s) 550
HaeIII GGCC 3 cut(s) 44, 74, 84
HgaI GACGC 1 cut(s) 248
HhaI GCGC 2 cut(s) 411, 549
Hin1I GRCGYC 1 cut(s) 547
Hin1II CATG 2 cut(s) 336, 629
Hin6I GCGC 2 cut(s) 409, 547
HinP1I GCGC 2 cut(s) 409, 547
HincII GTYRAC 2 cut(s) 394, 1042
HindII GTYRAC 2 cut(s) 394, 1042
HinfI GANTC 2 cut(s) 512, 734
HphI GGTGA 5 cut(s) 389, 608, 704, 1013, 1076
Hpy166II GTNNAC 4 cut(s) 394, 1042, 1053, 1114
Hpy188I TCNGA 3 cut(s) 327, 667, 768
Hpy188III TCNNGA 4 cut(s) 509, 641, 803, 974
Hpy8I GTNNAC 4 cut(s) 394, 1042, 1053, 1114
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 25, 229, 277, 819
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 9 cut(s) 138, 332, 336, 466, 629, 701, 838, 980, 1124
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 98, 408
HpyF3I CTNAG 3 cut(s) 67, 342, 1098
HpySE526I ACGT 1 cut(s) 53
Hsp92I GRCGYC 1 cut(s) 547
Hsp92II CATG 2 cut(s) 336, 629
HspAI GCGC 2 cut(s) 409, 547
KasI GGCGCC 1 cut(s) 546
Kzo9I GATC 4 cut(s) 773, 799, 814, 1003
Lsp1109I GCAGC 5 cut(s) 346, 348, 349, 418, 856
LweI GCATC 2 cut(s) 125, 967
MabI ACCWGGT 1 cut(s) 965
MaeI CTAG 3 cut(s) 521, 990, 1079
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 2 cut(s) 35, 178
MalI GATC 4 cut(s) 775, 801, 816, 1005
MboI GATC 4 cut(s) 773, 799, 814, 1003
MboII GAAGA 2 cut(s) 924, 998
MflI RGATCY 1 cut(s) 773
MlsI TGGCCA 1 cut(s) 74
MluCI AATT 3 cut(s) 467, 565, 645
MluNI TGGCCA 1 cut(s) 74
Mly113I GGCGCC 1 cut(s) 547
MnlI CCTC 8 cut(s) 76, 95, 97, 154, 307, 497, 774, 902
Mox20I TGGCCA 1 cut(s) 74
MscI TGGCCA 1 cut(s) 74
MseI TTAA 5 cut(s) 243, 384, 425, 609, 714
MslI CAYNNNNRTG 1 cut(s) 187
Msp20I TGGCCA 1 cut(s) 74
MspR9I CCNGG 4 cut(s) 81, 201, 967, 1062
MvaI CCWGG 4 cut(s) 81, 201, 967, 1062
MwoI GCNNNNNNNGC 3 cut(s) 71, 98, 408
NarI GGCGCC 1 cut(s) 547
NdeII GATC 4 cut(s) 773, 799, 814, 1003
NlaIII CATG 2 cut(s) 336, 629
NlaIV GGNNCC 2 cut(s) 12, 548
NmeAIII GCCGAG 1 cut(s) 127
NmuCI GTSAC 1 cut(s) 35
NspI RCATGY 1 cut(s) 336
NspV TTCGAA 1 cut(s) 515
PaeI GCATGC 1 cut(s) 336
PfeI GAWTC 2 cut(s) 512, 734
PkrI GCNGC 7 cut(s) 100, 338, 361, 364, 408, 871, 995
PluTI GGCGCC 1 cut(s) 550
PmaCI CACGTG 1 cut(s) 54
PmlI CACGTG 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 54
PshBI ATTAAT 1 cut(s) 714
Psp6I CCWGG 4 cut(s) 79, 199, 965, 1060
PspCI CACGTG 1 cut(s) 54
PspGI CCWGG 4 cut(s) 79, 199, 965, 1060
PspN4I GGNNCC 2 cut(s) 12, 548
PspPI GGNCC 2 cut(s) 10, 963
PsuI RGATCY 1 cut(s) 773
RsaI GTAC 1 cut(s) 1054
RsaNI GTAC 1 cut(s) 1053
RseI CAYNNNNRTG 1 cut(s) 187
SaqAI TTAA 5 cut(s) 243, 384, 425, 609, 714
SatI GCNGC 7 cut(s) 99, 337, 360, 363, 407, 870, 994
Sau3AI GATC 4 cut(s) 773, 799, 814, 1003
Sau96I GGNCC 2 cut(s) 10, 963
ScrFI CCNGG 4 cut(s) 81, 201, 967, 1062
SexAI ACCWGGT 1 cut(s) 965
SfaNI GCATC 2 cut(s) 125, 967
SfoI GGCGCC 1 cut(s) 548
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 2 cut(s) 10, 963
SmiMI CAYNNNNRTG 1 cut(s) 187
SphI GCATGC 1 cut(s) 336
Sse9I AATT 3 cut(s) 467, 565, 645
SsiI CCGC 6 cut(s) 48, 99, 375, 858, 930, 993
SspDI GGCGCC 1 cut(s) 546
SspMI CTAG 3 cut(s) 521, 990, 1079
StyD4I CCNGG 4 cut(s) 79, 199, 965, 1060
StyI CCWWGG 3 cut(s) 476, 485, 540
TaiI ACGT 1 cut(s) 56
TaqI TCGA 5 cut(s) 510, 515, 720, 746, 1023
TasI AATT 3 cut(s) 467, 565, 645
TauI GCSGC 2 cut(s) 101, 996
TfiI GAWTC 2 cut(s) 512, 734
Tru1I TTAA 5 cut(s) 243, 384, 425, 609, 714
Tru9I TTAA 5 cut(s) 243, 384, 425, 609, 714
TscAI CASTG 5 cut(s) 75, 100, 466, 608, 1003
TseFI GTSAC 1 cut(s) 35
TseI GCWGC 5 cut(s) 336, 359, 362, 406, 869
Tsp45I GTSAC 1 cut(s) 35
TspDTI ATGAA 1 cut(s) 222
TspGWI ACGGA 1 cut(s) 23
TspRI CASTG 5 cut(s) 75, 100, 466, 608, 1003
VpaK11BI GGWCC 2 cut(s) 10, 963
VspI ATTAAT 1 cut(s) 714
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 1 cut(s) 565
XceI RCATGY 1 cut(s) 336
XmiI GTMKAC 1 cut(s) 1113
XspI CTAG 3 cut(s) 521, 990, 1079
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.