AT5G46640

DNA-binding family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013533
Physical Location & Seq
Forward (+)
18924429 .. 18926984
2556 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46640.1

Sequence Viewer

Length: 1161 bp
ATGGATTCCAGAGACATCCCACCGTCACATAACCAGCTTCAACCACCACCGGGAATGTTAATGTCTCATTACCGTAACCCTAACGCCGCCGCTTCACCATTAATGGTTCCCACTTCCACATCTCAACCGATTCAACACCCTCGTCTTCCTTTTGGCAATCAACAACAATCTCAAACGTTTCATCAGCAGCAACAACAACAAATGGATCAGAAGACTCTTGAATCTCTTGGATTTGGTGATGGATCACCTTCTTCTCAACCGATGCGATTCGGGATCGATGATCAGAATCAGCAACTGCAAGTGAAGAAGAAGCGAGGAAGGCCGAGAAAGTATACTCCTGATGGTAGCATTGCTTTAGGTTTAGCTCCTACGTCTCCTCTTCTCTCTGCAGCTTCTAATTCTTACGGTGAGGGTGGTGTTGGAGATAGTGGTGGAAATGGAAACTCTGTTGATCCACCTGTTAAACGTAACAGAGGAAGGCCTCCTGGTTCTAGTAAGAAACAGCTTGATGCTTTAGGAGGAACTTCAGGAGTTGGGTTTACACCTCATGTCATTGAAGTGAACACAGGAGAGGACATAGCGTCAAAGGTGATGGCTTTTTCGGATCAAGGGTCAAGAACAATTTGTATTCTCTCTGCAAGTGGTGCAGTTTCTAGAGTGATGCTTCGTCAAGCTTCTCATTCTAGTGGAATCGTTACTTATGAGGGACGATTTGAGATCATTACTCTCTCAGGCTCAGTCTTGAATTATGAGGTAAATGGTTCCACCAACAGAAGTGGTAACTTGAGTGTGGCTTTGGCTGGACCTGATGGCGGCATCGTAGGTGGCAGTGTAGTTGGTAATCTAGTAGCTGCAACACAAGTCCAGGTGATAGTGGGAAGCTTTGTTGCAGAAGCAAAGAAACCGAAACAAAGTAGTGTTAACATTGCTCGGGGGCAGAATCCTGAACCGGCTTCAGCGCCGGCTAACATGTTGAACTTTGGATCAGTCTCTCAAGGACCATCGAGCGAGTCATCAGAAGAGAATGAGAGCGGTTCTCCTGCAATGCACCGTGACAATAATAATGGGATATATGGAGCTCAACAACAACAACAACAACAACCTCTTCATCCTCATCAGATGCAAATGTACCAACATCTTTGGTCTAATCATGGTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

386

Amino Acids

40.83

Weight (kDa)

8.61

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 181 - 296 6e-23 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17950 AT5G46640
fragaria_vesca FvH4_5g22000
malus_domestica MD06G1033700.v1.1 MD16G1274800.v1.1
prunus_persica Prupe.5G037000_v2.0.a1
pyrus_communis pycom06g02780 pycom16g24490
rosa_chinensis RchiOBHm_Chr7g0209501
rosa_laevigata RLG00000003123
rosa_multiflora Rmu_sc0000243.1_g000005
rosa_roxburghii Rroxscaffold_3G00249310
rosa_rugosa Rorug07G0114000
rosa_samantha Rh7AG248000 Rh7BG242500 Rh7CG263500 Rh7DG254100
rosa_wichuraiana Rw7G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1032
AccI GTMKAC 1 cut(s) 332
AciI CCGC 4 cut(s) 87, 90, 813, 1032
AclI AACGTT 1 cut(s) 176
AclWI GGATC 6 cut(s) 213, 250, 281, 446, 612, 991
AcuI CTGAAG 2 cut(s) 510, 939
AfaI GTAC 1 cut(s) 1130
AfiI CCNNNNNNNGG 2 cut(s) 50, 812
AflIII ACRYGT 1 cut(s) 969
AgsI TTSAA 6 cut(s) 41, 134, 221, 557, 745, 976
AhdI GACNNNNNGTC 1 cut(s) 580
AjnI CCWGG 2 cut(s) 484, 864
AluBI AGCT 8 cut(s) 37, 365, 392, 505, 674, 851, 882, 1079
AluI AGCT 8 cut(s) 37, 365, 392, 505, 674, 851, 882, 1079
Alw21I GWGCWC 1 cut(s) 1081
Alw26I GTCTC 4 cut(s) 6, 69, 378, 994
AlwI GGATC 6 cut(s) 213, 250, 281, 446, 612, 991
AlwNI CAGNNNCTG 1 cut(s) 295
Ama87I CYCGRG 1 cut(s) 930
AoxI GGCC 2 cut(s) 320, 479
ApeKI GCWGC 3 cut(s) 187, 389, 851
AseI ATTAAT 1 cut(s) 101
AspLEI GCGC 1 cut(s) 961
AspS9I GGNCC 2 cut(s) 803, 998
AsuC2I CCSGG 1 cut(s) 51
AsuHPI GGTGA 6 cut(s) 87, 237, 248, 419, 601, 880
AvaI CYCGRG 1 cut(s) 930
AvaII GGWCC 2 cut(s) 803, 998
BanII GRGCYC 1 cut(s) 1081
BbsI GAAGAC 2 cut(s) 137, 218
Bbv12I GWGCWC 1 cut(s) 1081
BbvI GCAGC 3 cut(s) 199, 401, 838
BccI CCATC 5 cut(s) 233, 335, 586, 803, 1009
BciT130I CCWGG 2 cut(s) 486, 866
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 1 cut(s) 51
BcoDI GTCTC 4 cut(s) 6, 69, 378, 994
BfaI CTAG 4 cut(s) 492, 654, 684, 845
BfmI CTRYAG 1 cut(s) 387
BfoI RGCGCY 1 cut(s) 962
BisI GCNGC 6 cut(s) 87, 90, 188, 390, 814, 852
BlsI GCNGC 6 cut(s) 88, 91, 189, 391, 815, 853
Bme1390I CCNGG 3 cut(s) 51, 486, 866
Bme18I GGWCC 2 cut(s) 803, 998
BmeRI GACNNNNNGTC 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 930
BmgT120I GGNCC 2 cut(s) 803, 998
BmiI GGNNCC 2 cut(s) 108, 763
BmrFI CCNGG 3 cut(s) 51, 486, 866
BmsI GCATC 5 cut(s) 252, 499, 651, 825, 1110
BpiI GAAGAC 2 cut(s) 137, 218
BplI GAGNNNNNCTC 2 cut(s) 1021, 1053
BpuEI CTTGAG 2 cut(s) 805, 978
BpuMI CCSGG 1 cut(s) 51
Bsa29I ATCGAT 1 cut(s) 276
BsaBI GATNNNNATC 1 cut(s) 285
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 812
Bse118I RCCGGY 2 cut(s) 949, 961
Bse3DI GCAATG 3 cut(s) 348, 924, 1050
Bse8I GATNNNNATC 1 cut(s) 285
BseBI CCWGG 2 cut(s) 486, 866
BseCI ATCGAT 1 cut(s) 276
BseGI GGATG 2 cut(s) 15, 1108
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 2 cut(s) 50, 812
BseMI GCAATG 3 cut(s) 348, 924, 1050
BseMII CTCAG 2 cut(s) 744, 750
BseRI GAGGAG 1 cut(s) 366
BseXI GCAGC 3 cut(s) 199, 401, 838
BsgI GTGCAG 1 cut(s) 666
BshFI GGCC 2 cut(s) 322, 481
BshVI ATCGAT 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 1081
BsiHKCI CYCGRG 1 cut(s) 930
BsiSI CCGG 3 cut(s) 50, 950, 962
BslFI GGGAC 1 cut(s) 720
BslI CCNNNNNNNGG 2 cut(s) 50, 812
BsmAI GTCTC 4 cut(s) 6, 69, 378, 994
BsmBI CGTCTC 1 cut(s) 378
BsmFI GGGAC 1 cut(s) 720
BsnI GGCC 2 cut(s) 322, 481
BsoBI CYCGRG 1 cut(s) 930
Bsp1286I GDGCHC 1 cut(s) 1081
Bsp143I GATC 8 cut(s) 205, 242, 273, 280, 451, 604, 717, 983
BspACI CCGC 4 cut(s) 87, 90, 813, 1032
BspANI GGCC 2 cut(s) 322, 481
BspCNI CTCAG 2 cut(s) 743, 749
BspDI ATCGAT 1 cut(s) 276
BspLI GGNNCC 2 cut(s) 108, 763
BspMAI CTGCAG 1 cut(s) 391
BspPI GGATC 6 cut(s) 213, 250, 281, 446, 612, 991
BsrBI CCGCTC 1 cut(s) 1032
BsrDI GCAATG 3 cut(s) 348, 924, 1050
BsrFI RCCGGY 2 cut(s) 949, 961
BssAI RCCGGY 2 cut(s) 949, 961
BssMI GATC 8 cut(s) 205, 242, 273, 280, 451, 604, 717, 983
BssNAI GTATAC 1 cut(s) 333
Bst1107I GTATAC 1 cut(s) 333
Bst2UI CCWGG 2 cut(s) 486, 866
Bst4CI ACNGT 4 cut(s) 24, 74, 407, 1052
Bst6I CTCTTC 3 cut(s) 384, 1014, 1110
BstAPI GCANNNNNTGC 1 cut(s) 644
BstC8I GCNNGC 1 cut(s) 963
BstDEI CTNAG 2 cut(s) 730, 736
BstF5I GGATG 2 cut(s) 15, 1108
BstH2I RGCGCY 1 cut(s) 962
BstHHI GCGC 1 cut(s) 961
BstKTI GATC 8 cut(s) 208, 245, 276, 283, 454, 607, 720, 986
BstMAI GTCTC 4 cut(s) 6, 69, 378, 994
BstMBI GATC 8 cut(s) 205, 242, 273, 280, 451, 604, 717, 983
BstMWI GCNNNNNNNGC 2 cut(s) 319, 644
BstNI CCWGG 2 cut(s) 486, 866
BstNSI RCATGY 1 cut(s) 973
BstSCI CCNGG 3 cut(s) 49, 484, 864
BstSFI CTRYAG 1 cut(s) 387
BstV1I GCAGC 3 cut(s) 199, 401, 838
BstV2I GAAGAC 2 cut(s) 137, 218
BstZ17I GTATAC 1 cut(s) 333
Bsu15I ATCGAT 1 cut(s) 276
BsuRI GGCC 2 cut(s) 322, 481
BsuTUI ATCGAT 1 cut(s) 276
BtsCI GGATG 2 cut(s) 15, 1108
BtsI GCAGTG 1 cut(s) 835
BtsIMutI CAGTG 1 cut(s) 835
Cac8I GCNNGC 1 cut(s) 963
CaiI CAGNNNCTG 1 cut(s) 295
CfoI GCGC 1 cut(s) 961
Cfr10I RCCGGY 2 cut(s) 949, 961
Cfr13I GGNCC 2 cut(s) 803, 998
ClaI ATCGAT 1 cut(s) 276
CseI GACGC 1 cut(s) 570
Csp6I GTAC 1 cut(s) 1129
CspCI CAANNNNNGTGG 2 cut(s) 757, 792
CviAII CATG 3 cut(s) 548, 970, 1151
CviQI GTAC 1 cut(s) 1129
DdeI CTNAG 2 cut(s) 730, 736
DpnI GATC 8 cut(s) 207, 244, 275, 282, 453, 606, 719, 985
DpnII GATC 8 cut(s) 205, 242, 273, 280, 451, 604, 717, 983
DriI GACNNNNNGTC 1 cut(s) 580
Eam1104I CTCTTC 3 cut(s) 384, 1014, 1110
Eam1105I GACNNNNNGTC 1 cut(s) 580
EarI CTCTTC 3 cut(s) 384, 1014, 1110
Ecl136II GAGCTC 1 cut(s) 1079
Eco147I AGGCCT 1 cut(s) 481
Eco24I GRGCYC 1 cut(s) 1081
Eco47I GGWCC 2 cut(s) 803, 998
Eco53kI GAGCTC 1 cut(s) 1079
Eco57I CTGAAG 2 cut(s) 510, 939
Eco88I CYCGRG 1 cut(s) 930
EcoICRI GAGCTC 1 cut(s) 1079
EcoRII CCWGG 2 cut(s) 484, 864
EcoT38I GRGCYC 1 cut(s) 1081
Esp3I CGTCTC 1 cut(s) 378
FaeI CATG 3 cut(s) 551, 973, 1154
FaqI GGGAC 1 cut(s) 720
FatI CATG 3 cut(s) 547, 969, 1150
FbaI TGATCA 1 cut(s) 280
FblI GTMKAC 1 cut(s) 332
Fnu4HI GCNGC 6 cut(s) 87, 90, 188, 390, 814, 852
FokI GGATG 2 cut(s) 2, 1095
FriOI GRGCYC 1 cut(s) 1081
Fsp4HI GCNGC 6 cut(s) 87, 90, 188, 390, 814, 852
FspBI CTAG 4 cut(s) 492, 654, 684, 845
GlaI GCGC 1 cut(s) 960
GluI GCNGC 6 cut(s) 87, 90, 188, 390, 814, 852
HaeII RGCGCY 1 cut(s) 962
HaeIII GGCC 2 cut(s) 322, 481
HapII CCGG 3 cut(s) 50, 950, 962
HgaI GACGC 1 cut(s) 570
HhaI GCGC 1 cut(s) 961
Hin1II CATG 3 cut(s) 551, 973, 1154
Hin6I GCGC 1 cut(s) 959
HinP1I GCGC 1 cut(s) 959
HincII GTYRAC 1 cut(s) 922
HindII GTYRAC 1 cut(s) 922
HindIII AAGCTT 2 cut(s) 672, 880
HinfI GANTC 9 cut(s) 5, 130, 214, 221, 267, 286, 690, 940, 1010
HpaI GTTAAC 1 cut(s) 922
HpaII CCGG 3 cut(s) 50, 950, 962
HphI GGTGA 6 cut(s) 87, 237, 248, 419, 601, 880
Hpy166II GTNNAC 4 cut(s) 333, 540, 562, 922
Hpy188I TCNGA 5 cut(s) 210, 285, 604, 1018, 1119
Hpy188III TCNNGA 9 cut(s) 9, 218, 271, 338, 528, 615, 654, 742, 944
Hpy8I GTNNAC 4 cut(s) 333, 540, 562, 922
HpyAV CCTTC 3 cut(s) 258, 312, 471
HpyCH4III ACNGT 4 cut(s) 24, 74, 407, 1052
HpyCH4IV ACGT 3 cut(s) 176, 371, 466
HpyCH4V TGCA 9 cut(s) 298, 389, 638, 647, 854, 890, 1043, 1048, 1123
HpyF10VI GCNNNNNNNGC 2 cut(s) 319, 644
HpyF3I CTNAG 2 cut(s) 730, 736
HpySE526I ACGT 3 cut(s) 176, 371, 466
Hsp92II CATG 3 cut(s) 551, 973, 1154
HspAI GCGC 1 cut(s) 959
KroI GCCGGC 1 cut(s) 961
KroNI GCCGGC 1 cut(s) 963
Ksp22I TGATCA 1 cut(s) 280
KspAI GTTAAC 1 cut(s) 922
Kzo9I GATC 8 cut(s) 205, 242, 273, 280, 451, 604, 717, 983
LmnI GCTCC 2 cut(s) 370, 1076
Lsp1109I GCAGC 3 cut(s) 199, 401, 838
LweI GCATC 5 cut(s) 252, 499, 651, 825, 1110
MaeI CTAG 4 cut(s) 492, 654, 684, 845
MaeII ACGT 3 cut(s) 176, 371, 466
MaeIII GTNAC 6 cut(s) 24, 74, 467, 694, 779, 1052
MalI GATC 8 cut(s) 207, 244, 275, 282, 453, 606, 719, 985
MbiI CCGCTC 1 cut(s) 1032
MboI GATC 8 cut(s) 205, 242, 273, 280, 451, 604, 717, 983
MboII GAAGA 8 cut(s) 137, 223, 243, 316, 319, 371, 1031, 1097
MhlI GDGCHC 1 cut(s) 1081
MluCI AATT 3 cut(s) 397, 621, 745
MlyI GAGTC 2 cut(s) 208, 1019
MmeI TCCRAC 1 cut(s) 400
MroNI GCCGGC 1 cut(s) 961
MseI TTAA 4 cut(s) 59, 101, 462, 921
MslI CAYNNNNRTG 2 cut(s) 557, 684
MspI CCGG 3 cut(s) 50, 950, 962
MspR9I CCNGG 3 cut(s) 51, 486, 866
MvaI CCWGG 2 cut(s) 486, 866
MwoI GCNNNNNNNGC 2 cut(s) 319, 644
NaeI GCCGGC 1 cut(s) 963
NciI CCSGG 1 cut(s) 51
NdeII GATC 8 cut(s) 205, 242, 273, 280, 451, 604, 717, 983
NgoMIV GCCGGC 1 cut(s) 961
NlaIII CATG 3 cut(s) 551, 973, 1154
NlaIV GGNNCC 2 cut(s) 108, 763
NmeAIII GCCGAG 1 cut(s) 348
NmuCI GTSAC 2 cut(s) 24, 1052
NspI RCATGY 1 cut(s) 973
PceI AGGCCT 1 cut(s) 481
PciI ACATGT 1 cut(s) 969
PdiI GCCGGC 1 cut(s) 963
PfeI GAWTC 7 cut(s) 5, 130, 221, 267, 286, 690, 940
PkrI GCNGC 6 cut(s) 88, 91, 189, 391, 815, 853
PleI GAGTC 2 cut(s) 208, 1018
PpsI GAGTC 2 cut(s) 208, 1018
PscI ACATGT 1 cut(s) 969
PshBI ATTAAT 1 cut(s) 101
Psp124BI GAGCTC 1 cut(s) 1081
Psp1406I AACGTT 1 cut(s) 176
Psp6I CCWGG 2 cut(s) 484, 864
PspGI CCWGG 2 cut(s) 484, 864
PspN4I GGNNCC 2 cut(s) 108, 763
PspPI GGNCC 2 cut(s) 803, 998
PstI CTGCAG 1 cut(s) 391
PstNI CAGNNNCTG 1 cut(s) 295
RsaI GTAC 1 cut(s) 1130
RsaNI GTAC 1 cut(s) 1129
RseI CAYNNNNRTG 2 cut(s) 557, 684
SacI GAGCTC 1 cut(s) 1081
SaqAI TTAA 4 cut(s) 59, 101, 462, 921
SatI GCNGC 6 cut(s) 87, 90, 188, 390, 814, 852
Sau3AI GATC 8 cut(s) 205, 242, 273, 280, 451, 604, 717, 983
Sau96I GGNCC 2 cut(s) 803, 998
SchI GAGTC 2 cut(s) 208, 1019
ScrFI CCNGG 3 cut(s) 51, 486, 866
SduI GDGCHC 1 cut(s) 1081
SfaNI GCATC 5 cut(s) 252, 499, 651, 825, 1110
SfcI CTRYAG 1 cut(s) 387
SinI GGWCC 2 cut(s) 803, 998
SmiMI CAYNNNNRTG 2 cut(s) 557, 684
SmlI CTYRAG 2 cut(s) 784, 993
SmoI CTYRAG 2 cut(s) 784, 993
Sse9I AATT 3 cut(s) 397, 621, 745
SseBI AGGCCT 1 cut(s) 481
SsiI CCGC 4 cut(s) 87, 90, 813, 1032
SspMI CTAG 4 cut(s) 492, 654, 684, 845
SstI GAGCTC 1 cut(s) 1081
StuI AGGCCT 1 cut(s) 481
StyD4I CCNGG 3 cut(s) 49, 484, 864
TaaI ACNGT 4 cut(s) 24, 74, 407, 1052
TaiI ACGT 3 cut(s) 179, 374, 469
TaqI TCGA 2 cut(s) 276, 1004
TasI AATT 3 cut(s) 397, 621, 745
TauI GCSGC 3 cut(s) 89, 92, 816
TfiI GAWTC 7 cut(s) 5, 130, 221, 267, 286, 690, 940
Tru1I TTAA 4 cut(s) 59, 101, 462, 921
Tru9I TTAA 4 cut(s) 59, 101, 462, 921
TscAI CASTG 1 cut(s) 835
TseFI GTSAC 2 cut(s) 24, 1052
TseI GCWGC 3 cut(s) 187, 389, 851
Tsp45I GTSAC 2 cut(s) 24, 1052
TspDTI ATGAA 2 cut(s) 170, 1097
TspRI CASTG 1 cut(s) 835
VpaK11BI GGWCC 2 cut(s) 803, 998
VspI ATTAAT 1 cut(s) 101
XbaI TCTAGA 1 cut(s) 653
XceI RCATGY 1 cut(s) 973
XmiI GTMKAC 1 cut(s) 332
XspI CTAG 4 cut(s) 492, 654, 684, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.