Prupe.5G037000_v2.0.a1

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0013533
Physical Location & Seq
Forward (+)
4177031 .. 4182789
5759 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G037000.1

Sequence Viewer

Length: 1134 bp
ATGGATTCGCGGGAAGTTCCGCAGCAGCAGCAGCCGCCGCCGCCTCCTCAGCAGCAGAGCATGATGGTGGGTCCGCCTTCCTACCAAACGTCCATGCCCAACAGCAACCTAAACCCCAATTCGGGTCCCATGATGGGCGGCCCTAATCCGGCCCGGTTCCCGTTCAATGCCGTGCCTCAGCCGCAGCAGCAGCAGCAGCAGCCCACATCGAAGCCGCAGATGGATTCTCTGAGCCCCAGTCCGTACGACGGGTCGCTGAGGCCCTGCGGCAGCGGCGGAGGGTTCAGCATCGACTCTTCTTCGGCTTCCGCGGCGAAGAAGAAGCGCGGGCGGCCCAGAAAGTACTCACCCGACGGCAACATTGCGTTGGGATTGGCTCCCACACAGATGCCCTCGACGGCTTCGACAGCCGCGGCCGGGCCCCATGGGGAGTCGAGTGGCACAATGTCTTCGGACCCCCCGGCCAAGAAGAACCGAGGGAGGCCCCCCGGTTCGGGCAAGAAGCAGTTGGACGCTTTGGGAGCTGGTGGGGTTGGGTTCACGCCTCATGTTATTATGGTGCAAGCCGGCGAGGATATTGCAGCAAAGGTTATGTCCTTCTCACAACAAGGACCGCGTACGGTTTGTATTCTCTCTGCAAATGGGGCCATCTGCAATGTTACCCTTCGCCAGCCAGCAATGTCCGGTGGTACTGTCACATATGAGGGTCGATTTGAGATTATTTCGCTCTCAGGTTCCTACTTGTTTTCTGAAAATAATGGGAACCGCAGTAGAAGTGGTGGTTTGAGTGTATCCTTGGCAGGGTCTGATGGCCAAGTTTTGGGTGGTGGAGTTGCGGGCATGCTGGTGGCAGCATCACCGGTACAGGTCATTGTGGGTAGTTTTATTGCTGATGGGAAAAAATCCAACTCCAACTTCCTAAAATCCGGGCCTTCTTCTCCACCGCCATCTCAAATGTTGAATTTTGGTGCACCGATGACAGCGGCCAGCCCCTCCTCTCAAGGAGCCTCAAGTGAGTCATCTGATGAGAATGGTAGTAGCCCTCTCAACAGGGGACCTGTGCTCTACAATAATGCTAGTCAACCCATTCATAATATGCAGATGTACCAACTATGGGGTCAAGCTCAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

378

Amino Acids

38.8

Weight (kDa)

9.49

Isoelectric Point (pI)

65.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17950 AT5G46640
fragaria_vesca FvH4_5g22000
malus_domestica MD06G1033700.v1.1 MD16G1274800.v1.1
prunus_persica Prupe.5G037000_v2.0.a1
pyrus_communis pycom06g02780 pycom16g24490
rosa_chinensis RchiOBHm_Chr7g0209501
rosa_laevigata RLG00000003123
rosa_multiflora Rmu_sc0000243.1_g000005
rosa_roxburghii Rroxscaffold_3G00249310
rosa_rugosa Rorug07G0114000
rosa_samantha Rh7AG248000 Rh7BG242500 Rh7CG263500 Rh7DG254100
rosa_wichuraiana Rw7G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 820
AccII CGCG 5 cut(s) 10, 311, 327, 413, 616
AcoI YGGCCR 4 cut(s) 414, 462, 811, 984
AcsI RAATTY 1 cut(s) 961
AfaI GTAC 6 cut(s) 245, 344, 619, 691, 864, 1106
AgeI ACCGGT 1 cut(s) 859
AgsI TTSAA 2 cut(s) 166, 961
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
AluBI AGCT 2 cut(s) 524, 1124
AluI AGCT 2 cut(s) 524, 1124
Alw21I GWGCWC 2 cut(s) 973, 1065
Alw44I GTGCAC 1 cut(s) 969
AlwNI CAGNNNCTG 1 cut(s) 806
ApaI GGGCCC 1 cut(s) 423
ApaLI GTGCAC 1 cut(s) 969
ApoI RAATTY 1 cut(s) 961
ArsI GACNNNNNNTTYG 2 cut(s) 578, 610
AsiGI ACCGGT 1 cut(s) 859
AspLEI GCGC 1 cut(s) 327
AsuC2I CCSGG 5 cut(s) 154, 418, 461, 489, 928
AsuHPI GGTGA 2 cut(s) 339, 849
AvaII GGWCC 5 cut(s) 71, 125, 454, 611, 1055
BaeGI GKGCMC 2 cut(s) 423, 973
BalI TGGCCA 1 cut(s) 813
BanII GRGCYC 2 cut(s) 236, 423
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 2 cut(s) 973, 1065
BbvCI CCTCAGC 3 cut(s) 48, 177, 257
BccI CCATC 7 cut(s) 58, 127, 214, 656, 803, 887, 955
BceAI ACGGC 3 cut(s) 155, 370, 414
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciVI GTATCC 1 cut(s) 802
BcnI CCSGG 5 cut(s) 154, 418, 461, 489, 928
BfaI CTAG 1 cut(s) 1077
BfuI GTATCC 1 cut(s) 802
BmcAI AGTACT 1 cut(s) 344
Bme1390I CCNGG 5 cut(s) 154, 418, 461, 489, 928
Bme18I GGWCC 5 cut(s) 71, 125, 454, 611, 1055
BmrFI CCNGG 5 cut(s) 154, 418, 461, 489, 928
BmrI ACTGGG 1 cut(s) 231
BmsI GCATC 3 cut(s) 297, 378, 863
BmuI ACTGGG 1 cut(s) 231
BpiI GAAGAC 1 cut(s) 441
Bpu10I CCTNAGC 3 cut(s) 48, 177, 257
BpuEI CTTGAG 2 cut(s) 984, 994
BpuMI CCSGG 5 cut(s) 154, 418, 461, 489, 928
BsaJI CCNNGG 7 cut(s) 309, 411, 424, 459, 475, 487, 795
BsaWI WCCGGW 2 cut(s) 683, 859
Bse118I RCCGGY 2 cut(s) 566, 859
Bse1I ACTGG 1 cut(s) 237
Bse3DI GCAATG 3 cut(s) 360, 661, 684
BseDI CCNNGG 7 cut(s) 309, 411, 424, 459, 475, 487, 795
BseMI GCAATG 3 cut(s) 360, 661, 684
BseMII CTCAG 5 cut(s) 62, 191, 221, 248, 744
BseNI ACTGG 1 cut(s) 237
BseRI GAGGAG 2 cut(s) 36, 985
BseSI GKGCMC 2 cut(s) 423, 973
BseX3I CGGCCG 1 cut(s) 414
Bsh1236I CGCG 5 cut(s) 10, 311, 327, 413, 616
Bsh1285I CGRYCG 1 cut(s) 417
BshTI ACCGGT 1 cut(s) 859
BsiEI CGRYCG 1 cut(s) 417
BsiHKAI GWGCWC 2 cut(s) 973, 1065
BsiSI CCGG 9 cut(s) 149, 154, 417, 461, 489, 567, 684, 860, 927
BsiWI CGTACG 2 cut(s) 243, 617
BslFI GGGAC 2 cut(s) 111, 1068
BsmFI GGGAC 2 cut(s) 111, 1068
Bsp120I GGGCCC 1 cut(s) 419
Bsp1286I GDGCHC 4 cut(s) 236, 423, 973, 1065
Bsp19I CCATGG 1 cut(s) 424
BspCNI CTCAG 5 cut(s) 61, 190, 222, 249, 743
BspFNI CGCG 5 cut(s) 10, 311, 327, 413, 616
BsrDI GCAATG 3 cut(s) 360, 661, 684
BsrFI RCCGGY 2 cut(s) 566, 859
BsrI ACTGG 1 cut(s) 237
BssAI RCCGGY 2 cut(s) 566, 859
BssECI CCNNGG 7 cut(s) 309, 411, 424, 459, 475, 487, 795
BssT1I CCWWGG 2 cut(s) 424, 795
Bst4CI ACNGT 3 cut(s) 622, 694, 1131
Bst6I CTCTTC 1 cut(s) 301
BstC8I GCNNGC 8 cut(s) 329, 564, 568, 671, 675, 838, 842, 988
BstDEI CTNAG 5 cut(s) 48, 177, 230, 257, 730
BstDSI CCRYGG 3 cut(s) 309, 411, 424
BstFNI CGCG 5 cut(s) 10, 311, 327, 413, 616
BstHHI GCGC 1 cut(s) 327
BstMCI CGRYCG 1 cut(s) 417
BstNSI RCATGY 1 cut(s) 844
BstSCI CCNGG 5 cut(s) 152, 416, 459, 487, 926
BstSLI GKGCMC 2 cut(s) 423, 973
BstUI CGCG 5 cut(s) 10, 311, 327, 413, 616
BstV2I GAAGAC 1 cut(s) 441
BstZI CGGCCG 1 cut(s) 414
BsuI GTATCC 1 cut(s) 802
BtgI CCRYGG 3 cut(s) 309, 411, 424
Cac8I GCNNGC 8 cut(s) 329, 564, 568, 671, 675, 838, 842, 988
CaiI CAGNNNCTG 1 cut(s) 806
CfoI GCGC 1 cut(s) 327
Cfr10I RCCGGY 2 cut(s) 566, 859
Cfr42I CCGCGG 2 cut(s) 312, 414
CseI GACGC 1 cut(s) 521
Csp6I GTAC 6 cut(s) 244, 343, 618, 690, 863, 1105
CspAI ACCGGT 1 cut(s) 859
CviAII CATG 6 cut(s) 61, 94, 130, 425, 548, 841
CviQI GTAC 6 cut(s) 244, 343, 618, 690, 863, 1105
DdeI CTNAG 5 cut(s) 48, 177, 230, 257, 730
EaeI YGGCCR 4 cut(s) 414, 462, 811, 984
EagI CGGCCG 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 301
EarI CTCTTC 1 cut(s) 301
EciI GGCGGA 2 cut(s) 63, 291
EclXI CGGCCG 1 cut(s) 414
Eco130I CCWWGG 2 cut(s) 424, 795
Eco24I GRGCYC 2 cut(s) 236, 423
Eco47I GGWCC 5 cut(s) 71, 125, 454, 611, 1055
Eco52I CGGCCG 1 cut(s) 414
EcoO109I RGGNCCY 5 cut(s) 125, 261, 420, 483, 1055
EcoT14I CCWWGG 2 cut(s) 424, 795
EcoT38I GRGCYC 2 cut(s) 236, 423
ErhI CCWWGG 2 cut(s) 424, 795
FaeI CATG 6 cut(s) 64, 97, 133, 428, 551, 844
FaqI GGGAC 2 cut(s) 111, 1068
FatI CATG 6 cut(s) 60, 93, 129, 424, 547, 840
FauI CCCGC 3 cut(s) 3, 320, 829
FauNDI CATATG 1 cut(s) 700
FriOI GRGCYC 2 cut(s) 236, 423
FspBI CTAG 1 cut(s) 1077
GlaI GCGC 1 cut(s) 326
HapII CCGG 9 cut(s) 149, 154, 417, 461, 489, 567, 684, 860, 927
HgaI GACGC 1 cut(s) 521
HhaI GCGC 1 cut(s) 327
Hin1II CATG 6 cut(s) 64, 97, 133, 428, 551, 844
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HincII GTYRAC 1 cut(s) 1082
HindII GTYRAC 1 cut(s) 1082
HinfI GANTC 5 cut(s) 5, 224, 293, 431, 1016
HpaII CCGG 9 cut(s) 149, 154, 417, 461, 489, 567, 684, 860, 927
HphI GGTGA 2 cut(s) 339, 849
Hpy166II GTNNAC 3 cut(s) 540, 971, 1082
Hpy188I TCNGA 5 cut(s) 231, 454, 751, 808, 1024
Hpy8I GTNNAC 3 cut(s) 540, 971, 1082
Hpy99I CGWCG 3 cut(s) 251, 356, 400
HpyAV CCTTC 4 cut(s) 87, 607, 674, 942
HpyCH4III ACNGT 3 cut(s) 622, 694, 1131
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 6 cut(s) 562, 581, 638, 654, 971, 1099
HpyF3I CTNAG 5 cut(s) 48, 177, 230, 257, 730
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 6 cut(s) 64, 97, 133, 428, 551, 844
HspAI GCGC 1 cut(s) 325
KflI GGGWCCC 1 cut(s) 125
KroI GCCGGC 1 cut(s) 566
KroNI GCCGGC 1 cut(s) 568
KspI CCGCGG 2 cut(s) 312, 414
LmnI GCTCC 3 cut(s) 382, 521, 1004
LweI GCATC 3 cut(s) 297, 378, 863
MaeI CTAG 1 cut(s) 1077
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 2 cut(s) 658, 694
MboII GAAGA 7 cut(s) 288, 291, 328, 331, 441, 481, 927
MhlI GDGCHC 4 cut(s) 236, 423, 973, 1065
MlsI TGGCCA 1 cut(s) 813
MluCI AATT 2 cut(s) 118, 961
MluNI TGGCCA 1 cut(s) 813
MlyI GAGTC 3 cut(s) 287, 440, 1025
MmeI TCCRAC 3 cut(s) 489, 930, 936
Mox20I TGGCCA 1 cut(s) 813
MroNI GCCGGC 1 cut(s) 566
MscI TGGCCA 1 cut(s) 813
MslI CAYNNNNRTG 3 cut(s) 65, 386, 845
Msp20I TGGCCA 1 cut(s) 813
MspA1I CMGCKG 4 cut(s) 273, 311, 413, 983
MspI CCGG 9 cut(s) 149, 154, 417, 461, 489, 567, 684, 860, 927
MspR9I CCNGG 5 cut(s) 154, 418, 461, 489, 928
MvnI CGCG 5 cut(s) 10, 311, 327, 413, 616
NaeI GCCGGC 1 cut(s) 568
NciI CCSGG 5 cut(s) 154, 418, 461, 489, 928
NcoI CCATGG 1 cut(s) 424
NdeI CATATG 1 cut(s) 700
NgoMIV GCCGGC 1 cut(s) 566
NlaIII CATG 6 cut(s) 64, 97, 133, 428, 551, 844
NmuCI GTSAC 1 cut(s) 694
NspI RCATGY 1 cut(s) 844
PaeI GCATGC 1 cut(s) 844
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PdiI GCCGGC 1 cut(s) 568
PfeI GAWTC 2 cut(s) 5, 224
Pfl23II CGTACG 2 cut(s) 243, 617
PflMI CCANNNNNTGG 1 cut(s) 820
PinAI ACCGGT 1 cut(s) 859
PleI GAGTC 3 cut(s) 287, 439, 1024
PpsI GAGTC 3 cut(s) 287, 439, 1024
PpuMI RGGWCCY 2 cut(s) 125, 1055
Psp5II RGGWCCY 2 cut(s) 125, 1055
PspLI CGTACG 2 cut(s) 243, 617
PspOMI GGGCCC 1 cut(s) 419
PspPPI RGGWCCY 2 cut(s) 125, 1055
PstNI CAGNNNCTG 1 cut(s) 806
RsaI GTAC 6 cut(s) 245, 344, 619, 691, 864, 1106
RsaNI GTAC 6 cut(s) 244, 343, 618, 690, 863, 1105
RseI CAYNNNNRTG 3 cut(s) 65, 386, 845
SacII CCGCGG 2 cut(s) 312, 414
ScaI AGTACT 1 cut(s) 344
SchI GAGTC 3 cut(s) 287, 440, 1025
ScrFI CCNGG 5 cut(s) 154, 418, 461, 489, 928
SduI GDGCHC 4 cut(s) 236, 423, 973, 1065
SetI ASST 8 cut(s) 92, 111, 526, 591, 736, 870, 1060, 1126
SfaNI GCATC 3 cut(s) 297, 378, 863
Sfr303I CCGCGG 2 cut(s) 312, 414
SgrBI CCGCGG 2 cut(s) 312, 414
SinI GGWCC 5 cut(s) 71, 125, 454, 611, 1055
SmiMI CAYNNNNRTG 3 cut(s) 65, 386, 845
SmlI CTYRAG 2 cut(s) 999, 1009
SmoI CTYRAG 2 cut(s) 999, 1009
SphI GCATGC 1 cut(s) 844
Sse9I AATT 2 cut(s) 118, 961
SspMI CTAG 1 cut(s) 1077
StyD4I CCNGG 5 cut(s) 152, 416, 459, 487, 926
StyI CCWWGG 2 cut(s) 424, 795
TaaI ACNGT 3 cut(s) 622, 694, 1131
TaiI ACGT 1 cut(s) 92
TaqI TCGA 6 cut(s) 209, 291, 395, 404, 434, 709
TasI AATT 2 cut(s) 118, 961
TatI WGTACW 1 cut(s) 342
TfiI GAWTC 2 cut(s) 5, 224
TseFI GTSAC 1 cut(s) 694
Tsp45I GTSAC 1 cut(s) 694
TspDTI ATGAA 1 cut(s) 1079
TspGWI ACGGA 1 cut(s) 231
Van91I CCANNNNNTGG 1 cut(s) 820
VneI GTGCAC 1 cut(s) 969
VpaK11BI GGWCC 5 cut(s) 71, 125, 454, 611, 1055
XapI RAATTY 1 cut(s) 961
XceI RCATGY 1 cut(s) 844
XcmI CCANNNNNNNNNTGG 1 cut(s) 821
XspI CTAG 1 cut(s) 1077
ZrmI AGTACT 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.