MD16G1274800.v1.1

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0013533
Physical Location & Seq
Forward (+)
36740397 .. 36755135
14739 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1274800.v1.1.491

Sequence Viewer

Length: 1149 bp
ATGGATTCGCGGGAAGTTCCGCAGCAACAGCCGCCTCAGCAGCCGAACATGATGGTGGGCCCATCTTCATATCCCTCCTCCATGCCCACCGCCAACATAAACCCTAATTCGGGCCCCATGATGGGCGGCCCCAACCCGGGACGCTTCCCTTTCAACGTGGTGGCCCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAGCCCGCTTCAAAACCGCAGATGGATTCTCTGAGCCCCAGCCCCTACGATGGGTCCTTGAGGCCGTGCGGCAGCGGTGGAGCGTTCAACATCGACTCTTCTTCCGCTTCGGCTGCCAAGAAGAAGAGAGGGCGGCCCAGGAAGTACTCGCCTGACGGCAACATTGCCTTGGGATTGACGCCCACGCAGATTCCTTCTTCGGCTTCGGCCGCACCTGGGACCCATGGAGAGTCTAGTGGCACGATGTCTTCGGAGCCCCCAGCCAAGAAGAACCGGGGGAGGCCCCCAGGTTCGGGCAAGAAGCAGTTGGACGCATTGGGAGCTGGCGGAGTTGGATTCACGCCTCATGTTATTATGGTGCAAGCCGGTGAGGATATAGCAGCAAAGGTTATGGCCTTTTCACAGCAGGGTCCACGCACAGTTTGCATTCTCTCTGCAAATGGTGCCATCTGCAATGTTACCCTTCGCCAGCCAGCAATGTCTGGTGGTACTGTCACATATGAGGGTCGATATGAGATTATTTCCCTGTCAGGTTCCTACTTGTTTTCTGAAAATAATGGGAATCGCAGCAGAAGTGGTGGTTTGAGTGTATCCTTAGCAGGGTCTGATGGTCAAGTTTTGGGAGGTGGAGTTGCTGGCATGCTAATGGCAGCATCACCAGTACAGGTGATTGTGGGTAGTTTCATTGCCGACGGGAAGAAATCCAACCCCAACTTGGTAAAGTCGGGAACTTCCTCTCCCCCAGCATCACAAATGTTGAACTTTGGTGCGCCAATGACAGCAGCCAGCCCGTCCTCTCAAGGAGGAGGGTCAAGCGAGTCGTCTGACGAGAATGGCAGCAGTCCTCTCAACAACAGCAACAGGGGACCTGTGCTCTACAGTAATGCAAATCAACCCATTCATAATATGCAGATGTATCAATTATGGGGTCAAGCTCAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

383

Amino Acids

39.37

Weight (kDa)

9.46

Isoelectric Point (pI)

65.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 182 - 297 2.5e-25 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17950 AT5G46640
fragaria_vesca FvH4_5g22000
malus_domestica MD06G1033700.v1.1 MD16G1274800.v1.1
prunus_persica Prupe.5G037000_v2.0.a1
pyrus_communis pycom06g02780 pycom16g24490
rosa_chinensis RchiOBHm_Chr7g0209501
rosa_laevigata RLG00000003123
rosa_multiflora Rmu_sc0000243.1_g000005
rosa_roxburghii Rroxscaffold_3G00249310
rosa_rugosa Rorug07G0114000
rosa_samantha Rh7AG248000 Rh7BG242500 Rh7CG263500 Rh7DG254100
rosa_wichuraiana Rw7G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 647
AccII CGCG 1 cut(s) 10
AcoI YGGCCR 1 cut(s) 411
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 3 cut(s) 350, 694, 867
AgsI TTSAA 4 cut(s) 154, 216, 292, 964
AjnI CCWGG 3 cut(s) 341, 418, 490
AloI GAACNNNNNNTCC 2 cut(s) 925, 957
AluBI AGCT 2 cut(s) 527, 1139
AluI AGCT 2 cut(s) 527, 1139
Alw21I GWGCWC 1 cut(s) 1080
AlwNI CAGNNNCTG 1 cut(s) 809
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 9 cut(s) 58, 112, 127, 162, 266, 338, 411, 485, 597
ApaI GGGCCC 2 cut(s) 62, 116
AspLEI GCGC 1 cut(s) 976
AsuC2I CCSGG 3 cut(s) 137, 138, 479
AsuHPI GGTGA 3 cut(s) 584, 852, 883
AvaI CYCGRG 1 cut(s) 136
AvaII GGWCC 4 cut(s) 258, 423, 614, 1070
BaeGI GKGCMC 2 cut(s) 62, 116
BanI GGYRCC 1 cut(s) 647
BanII GRGCYC 4 cut(s) 62, 116, 242, 462
BbsI GAAGAC 1 cut(s) 444
Bbv12I GWGCWC 1 cut(s) 1080
BbvCI CCTCAGC 1 cut(s) 36
BccI CCATC 7 cut(s) 46, 70, 115, 220, 248, 659, 806
BceAI ACGGC 2 cut(s) 253, 376
BciT130I CCWGG 3 cut(s) 343, 420, 492
BciVI GTATCC 1 cut(s) 805
BcnI CCSGG 3 cut(s) 137, 138, 479
BfaI CTAG 1 cut(s) 438
BfmI CTRYAG 1 cut(s) 1081
BfuI GTATCC 1 cut(s) 805
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 6 cut(s) 137, 138, 343, 420, 479, 492
Bme18I GGWCC 4 cut(s) 258, 423, 614, 1070
BmeT110I CYCGRG 1 cut(s) 136
BmrFI CCNGG 6 cut(s) 137, 138, 343, 420, 479, 492
BmsI GCATC 2 cut(s) 866, 959
BpiI GAAGAC 1 cut(s) 444
Bpu10I CCTNAGC 2 cut(s) 36, 799
BpuEI CTTGAG 2 cut(s) 283, 987
BpuMI CCSGG 3 cut(s) 137, 138, 479
BsaHI GRCGYC 1 cut(s) 383
BsaJI CCNNGG 7 cut(s) 136, 341, 372, 419, 427, 478, 490
BsaXI ACNNNNNCTCC 4 cut(s) 819, 849, 925, 955
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 1 cut(s) 863
Bse3DI GCAATG 4 cut(s) 366, 664, 687, 888
BseBI CCWGG 3 cut(s) 343, 420, 492
BseDI CCNNGG 7 cut(s) 136, 341, 372, 419, 427, 478, 490
BseMI GCAATG 4 cut(s) 366, 664, 687, 888
BseMII CTCAG 2 cut(s) 50, 227
BseNI ACTGG 1 cut(s) 863
BseRI GAGGAG 2 cut(s) 67, 1023
BseSI GKGCMC 2 cut(s) 62, 116
BseX3I CGGCCG 1 cut(s) 411
BseYI CCCAGC 4 cut(s) 165, 242, 463, 946
Bsh1236I CGCG 1 cut(s) 10
Bsh1285I CGRYCG 1 cut(s) 414
BshFI GGCC 9 cut(s) 60, 114, 129, 164, 268, 340, 413, 487, 599
BshNI GGYRCC 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 414
BsiHKAI GWGCWC 1 cut(s) 1080
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 3 cut(s) 137, 478, 570
BslFI GGGAC 3 cut(s) 153, 436, 1083
BsmFI GGGAC 3 cut(s) 153, 436, 1083
BsmI GAATGC 1 cut(s) 630
BsnI GGCC 9 cut(s) 60, 114, 129, 164, 268, 340, 413, 487, 599
BsoBI CYCGRG 1 cut(s) 136
Bsp120I GGGCCC 2 cut(s) 58, 112
Bsp1286I GDGCHC 5 cut(s) 62, 116, 242, 462, 1080
Bsp19I CCATGG 1 cut(s) 427
BspANI GGCC 9 cut(s) 60, 114, 129, 164, 268, 340, 413, 487, 599
BspCNI CTCAG 2 cut(s) 49, 228
BspFNI CGCG 1 cut(s) 10
BspT107I GGYRCC 1 cut(s) 647
BsrDI GCAATG 4 cut(s) 366, 664, 687, 888
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 1 cut(s) 863
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 7 cut(s) 136, 341, 372, 419, 427, 478, 490
BssNI GRCGYC 1 cut(s) 383
BssT1I CCWWGG 2 cut(s) 372, 427
Bst2UI CCWGG 3 cut(s) 343, 420, 492
Bst4CI ACNGT 3 cut(s) 625, 697, 1085
Bst6I CTCTTC 2 cut(s) 307, 323
BstACI GRCGYC 1 cut(s) 383
BstAPI GCANNNNNTGC 2 cut(s) 627, 647
BstC8I GCNNGC 8 cut(s) 210, 529, 567, 674, 678, 841, 845, 991
BstDEI CTNAG 3 cut(s) 36, 236, 799
BstDSI CCRYGG 1 cut(s) 427
BstFNI CGCG 1 cut(s) 10
BstHHI GCGC 1 cut(s) 976
BstMCI CGRYCG 1 cut(s) 414
BstNI CCWGG 3 cut(s) 343, 420, 492
BstNSI RCATGY 1 cut(s) 847
BstSCI CCNGG 6 cut(s) 135, 136, 341, 418, 477, 490
BstSFI CTRYAG 1 cut(s) 1081
BstSLI GKGCMC 2 cut(s) 62, 116
BstUI CGCG 1 cut(s) 10
BstV2I GAAGAC 1 cut(s) 444
BstZI CGGCCG 1 cut(s) 411
BsuI GTATCC 1 cut(s) 805
BsuRI GGCC 9 cut(s) 60, 114, 129, 164, 268, 340, 413, 487, 599
BtgI CCRYGG 1 cut(s) 427
Cac8I GCNNGC 8 cut(s) 210, 529, 567, 674, 678, 841, 845, 991
CaiI CAGNNNCTG 1 cut(s) 809
CfoI GCGC 1 cut(s) 976
Cfr10I RCCGGY 1 cut(s) 569
Cfr9I CCCGGG 1 cut(s) 136
CseI GACGC 3 cut(s) 150, 391, 524
Csp6I GTAC 3 cut(s) 349, 693, 866
CviAII CATG 6 cut(s) 49, 82, 118, 428, 551, 844
CviQI GTAC 3 cut(s) 349, 693, 866
DdeI CTNAG 3 cut(s) 36, 236, 799
EaeI YGGCCR 1 cut(s) 411
EagI CGGCCG 1 cut(s) 411
Eam1104I CTCTTC 2 cut(s) 307, 323
EarI CTCTTC 2 cut(s) 307, 323
EciI GGCGGA 1 cut(s) 546
EclXI CGGCCG 1 cut(s) 411
Eco130I CCWWGG 2 cut(s) 372, 427
Eco24I GRGCYC 4 cut(s) 62, 116, 242, 462
Eco47I GGWCC 4 cut(s) 258, 423, 614, 1070
Eco52I CGGCCG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 136
EcoO109I RGGNCCY 5 cut(s) 113, 258, 423, 486, 1070
EcoRII CCWGG 3 cut(s) 341, 418, 490
EcoT14I CCWWGG 2 cut(s) 372, 427
EcoT38I GRGCYC 4 cut(s) 62, 116, 242, 462
ErhI CCWWGG 2 cut(s) 372, 427
FaeI CATG 6 cut(s) 52, 85, 121, 431, 554, 847
FaqI GGGAC 3 cut(s) 153, 436, 1083
FatI CATG 6 cut(s) 48, 81, 117, 427, 550, 843
FauI CCCGC 2 cut(s) 3, 217
FauNDI CATATG 1 cut(s) 703
FriOI GRGCYC 4 cut(s) 62, 116, 242, 462
FspBI CTAG 1 cut(s) 438
GlaI GCGC 1 cut(s) 975
GsaI CCCAGC 4 cut(s) 169, 246, 467, 950
HaeIII GGCC 9 cut(s) 60, 114, 129, 164, 268, 340, 413, 487, 599
HapII CCGG 3 cut(s) 137, 478, 570
HgaI GACGC 3 cut(s) 150, 391, 524
HhaI GCGC 1 cut(s) 976
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 6 cut(s) 52, 85, 121, 431, 554, 847
Hin6I GCGC 1 cut(s) 974
HinP1I GCGC 1 cut(s) 974
HinfI GANTC 8 cut(s) 5, 230, 299, 394, 434, 540, 766, 1022
HpaII CCGG 3 cut(s) 137, 478, 570
HphI GGTGA 3 cut(s) 584, 852, 883
Hpy166II GTNNAC 1 cut(s) 617
Hpy188I TCNGA 5 cut(s) 237, 457, 754, 811, 1030
Hpy188III TCNNGA 1 cut(s) 930
Hpy8I GTNNAC 1 cut(s) 617
Hpy99I CGWCG 1 cut(s) 899
HpyAV CCTTC 2 cut(s) 408, 677
HpyCH4III ACNGT 3 cut(s) 625, 697, 1085
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 6 cut(s) 565, 630, 641, 657, 1091, 1114
HpyF3I CTNAG 3 cut(s) 36, 236, 799
HpySE526I ACGT 1 cut(s) 156
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 6 cut(s) 52, 85, 121, 431, 554, 847
HspAI GCGC 1 cut(s) 974
KflI GGGWCCC 1 cut(s) 423
LmnI GCTCC 3 cut(s) 284, 457, 524
LweI GCATC 2 cut(s) 866, 959
MaeI CTAG 1 cut(s) 438
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 2 cut(s) 661, 697
MboII GAAGA 9 cut(s) 57, 294, 297, 337, 340, 393, 444, 484, 913
MhlI GDGCHC 5 cut(s) 62, 116, 242, 462, 1080
MluCI AATT 2 cut(s) 106, 1124
MlyI GAGTC 3 cut(s) 293, 443, 1031
MmeI TCCRAC 3 cut(s) 492, 517, 933
MslI CAYNNNNRTG 2 cut(s) 53, 848
MspA1I CMGCKG 1 cut(s) 279
MspI CCGG 3 cut(s) 137, 478, 570
MspR9I CCNGG 6 cut(s) 137, 138, 343, 420, 479, 492
Mva1269I GAATGC 1 cut(s) 630
MvaI CCWGG 3 cut(s) 343, 420, 492
MvnI CGCG 1 cut(s) 10
NciI CCSGG 3 cut(s) 137, 138, 479
NcoI CCATGG 1 cut(s) 427
NdeI CATATG 1 cut(s) 703
NlaIII CATG 6 cut(s) 52, 85, 121, 431, 554, 847
NmuCI GTSAC 1 cut(s) 697
NspI RCATGY 1 cut(s) 847
PaeI GCATGC 1 cut(s) 847
PctI GAATGC 1 cut(s) 630
PfeI GAWTC 5 cut(s) 5, 230, 394, 540, 766
PleI GAGTC 3 cut(s) 293, 442, 1030
PpsI GAGTC 3 cut(s) 293, 442, 1030
PpuMI RGGWCCY 3 cut(s) 258, 423, 1070
Psp5II RGGWCCY 3 cut(s) 258, 423, 1070
Psp6I CCWGG 3 cut(s) 341, 418, 490
PspFI CCCAGC 4 cut(s) 165, 242, 463, 946
PspGI CCWGG 3 cut(s) 341, 418, 490
PspOMI GGGCCC 2 cut(s) 58, 112
PspPPI RGGWCCY 3 cut(s) 258, 423, 1070
PstNI CAGNNNCTG 1 cut(s) 809
RsaI GTAC 3 cut(s) 350, 694, 867
RsaNI GTAC 3 cut(s) 349, 693, 866
RseI CAYNNNNRTG 2 cut(s) 53, 848
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 3 cut(s) 293, 443, 1031
ScrFI CCNGG 6 cut(s) 137, 138, 343, 420, 479, 492
SduI GDGCHC 5 cut(s) 62, 116, 242, 462, 1080
SfaNI GCATC 2 cut(s) 866, 959
SfcI CTRYAG 1 cut(s) 1081
SinI GGWCC 4 cut(s) 258, 423, 614, 1070
SmaI CCCGGG 1 cut(s) 138
SmiMI CAYNNNNRTG 2 cut(s) 53, 848
SmlI CTYRAG 2 cut(s) 262, 1002
SmoI CTYRAG 2 cut(s) 262, 1002
SphI GCATGC 1 cut(s) 847
Sse9I AATT 2 cut(s) 106, 1124
SspMI CTAG 1 cut(s) 438
StyD4I CCNGG 6 cut(s) 135, 136, 341, 418, 477, 490
StyI CCWWGG 2 cut(s) 372, 427
TaaI ACNGT 3 cut(s) 625, 697, 1085
TaiI ACGT 1 cut(s) 159
TaqI TCGA 2 cut(s) 297, 712
TasI AATT 2 cut(s) 106, 1124
TatI WGTACW 2 cut(s) 348, 865
TauI GCSGC 5 cut(s) 34, 129, 276, 340, 416
TfiI GAWTC 5 cut(s) 5, 230, 394, 540, 766
TseFI GTSAC 1 cut(s) 697
Tsp45I GTSAC 1 cut(s) 697
TspDTI ATGAA 3 cut(s) 57, 877, 1094
TspMI CCCGGG 1 cut(s) 136
VpaK11BI GGWCC 4 cut(s) 258, 423, 614, 1070
XceI RCATGY 1 cut(s) 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 916
XmaI CCCGGG 1 cut(s) 136
XspI CTAG 1 cut(s) 438
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.