FvH4_5g22000

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0013533
Physical Location & Seq
Reverse (-)
13369767 .. 13375373
5607 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22000.t1

Sequence Viewer

Length: 1152 bp
ATGGATTCCCGGGAAGCCCCACCGCAGCCTCCGCCGCAGCAGCCACCTCAGCAGCAGCCCAACATCATGGTGGGCCCCAATTCCTACACCTCTCCCATCCCCAACAACACCGCCACCGCAACCAACAACAACAGCAACAGCTCCGCCGCGATGATCGGCGGGCCCAATTCGGGCCGGTTCCAGTACAACCCGGTGGCCCAGCAGCCGCCCGCATCGAAGCCGCTGGATGCCATGAGCCCTAGCCCGAGCCCCTTCGACGGATCGTTGAGGCCGTGCGGCAGCGGAGGGTTTAGCATCGACTCTTCCACGGCCTCGGCGGGGAAGAAGAAGCGTGGGAGGCCCAGGAAGTACTCTCCCGACGGCAACATTGCCTTGGGATTGGCGCCTACTCAGGTTGCTGCGTCGGCTGCTCCGGTTGCGGCCGCCGGCCCTCATGGCGAGTCGAGTGTCACCATGTCGTCGGATCCCCCTGCTAAGAAGAACAGGGGAAGGCCGCCGGGGTCTGGGAAGAAACAGCTCGACGCTTTGGGAGCTGGCGGAGTTGGGTTCACGCCTCATGTGATCTCGGTGCAAGCTGGCGAGGATATAGCAACGAAGGTTATGAACTTTTCACAGCAAGGACCTCGCACAATTTGTATCCTCTCTGCAAATGGTCCAATCAGCAATGTTACCCTTCGCCAGCCATCAATGTCTGGTGGTACAGTCACATATGAGGGTCGATTTGAGATTATTTCTCTGTCAGGCTCCTACATGTTCTCTGAAAATAATGGTAACCGCAGCAGAAGCGGTGGTTTGAGTGTGTCCCTGGCAGGGTCTGATGGTTCAGTGCTGGGTGGTGGAGTTGCAGGCATGCTAGTTGCAGCAGGCCCGGTACAGGTAATTGTGGGTAGTTTTATTGCGGAAGGGAAAAAATCTAGCTCCAATCTGCTAAAATCTGGGACTTCTTCCCCACCGCCATCTCAAATGTTGAACTTTGGTGCACCTATGACAGCGGCTAGCCCTTCCTCTCAAGGAGGAGGCTCAACTGAGTCATCTGATGAGAATGGTAGTAGCCCTCTTAACAGGGCGCCACCTGTGCTCTACAGTAACCCTAGTCAGCCCATGCATAATATGCAGATGTACCAAATATGGGCCGGGCAAACTCAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

384

Amino Acids

38.93

Weight (kDa)

9.57

Isoelectric Point (pI)

61.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 184 - 299 1.9e-25 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17950 AT5G46640
fragaria_vesca FvH4_5g22000
malus_domestica MD06G1033700.v1.1 MD16G1274800.v1.1
prunus_persica Prupe.5G037000_v2.0.a1
pyrus_communis pycom06g02780 pycom16g24490
rosa_chinensis RchiOBHm_Chr7g0209501
rosa_laevigata RLG00000003123
rosa_multiflora Rmu_sc0000243.1_g000005
rosa_roxburghii Rroxscaffold_3G00249310
rosa_rugosa Rorug07G0114000
rosa_samantha Rh7AG248000 Rh7BG242500 Rh7CG263500 Rh7DG254100
rosa_wichuraiana Rw7G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 382, 1066
AccII CGCG 1 cut(s) 149
AclWI GGATC 3 cut(s) 268, 458, 471
AcoI YGGCCR 1 cut(s) 420
AcyI GRCGYC 2 cut(s) 383, 1067
AfaI GTAC 5 cut(s) 185, 350, 700, 873, 1121
AfiI CCNNNNNNNGG 7 cut(s) 170, 257, 318, 503, 810, 874, 1129
AflIII ACRYGT 1 cut(s) 750
AgsI TTSAA 1 cut(s) 970
AjnI CCWGG 2 cut(s) 341, 804
AluBI AGCT 5 cut(s) 141, 517, 533, 575, 918
AluI AGCT 5 cut(s) 141, 517, 533, 575, 918
Alw21I GWGCWC 2 cut(s) 982, 1080
Alw44I GTGCAC 1 cut(s) 978
AlwI GGATC 3 cut(s) 268, 458, 471
AlwNI CAGNNNCTG 1 cut(s) 815
Ama87I CYCGRG 2 cut(s) 9, 244
ApaI GGGCCC 2 cut(s) 77, 165
ApaLI GTGCAC 1 cut(s) 978
AspLEI GCGC 2 cut(s) 385, 1069
AsuC2I CCSGG 6 cut(s) 10, 11, 191, 498, 869, 1135
AsuHPI GGTGA 1 cut(s) 442
AsuNHI GCTAGC 1 cut(s) 995
AvaI CYCGRG 2 cut(s) 9, 244
AvaII GGWCC 2 cut(s) 620, 653
BaeGI GKGCMC 3 cut(s) 77, 165, 982
BamHI GGATCC 1 cut(s) 463
BanI GGYRCC 2 cut(s) 382, 1066
BanII GRGCYC 4 cut(s) 77, 165, 239, 251
Bbv12I GWGCWC 2 cut(s) 982, 1080
BbvCI CCTCAGC 1 cut(s) 48
BccI CCATC 4 cut(s) 104, 691, 812, 964
BceAI ACGGC 3 cut(s) 256, 324, 376
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BciT130I CCWGG 2 cut(s) 343, 806
BciVI GTATCC 1 cut(s) 647
BcnI CCSGG 6 cut(s) 10, 11, 191, 498, 869, 1135
BfaI CTAG 5 cut(s) 240, 854, 915, 996, 1092
BfmI CTRYAG 1 cut(s) 1081
BfoI RGCGCY 2 cut(s) 386, 1070
BfuI GTATCC 1 cut(s) 647
BglI GCCNNNNNGGC 1 cut(s) 435
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 8 cut(s) 10, 11, 191, 343, 498, 806, 869, 1135
Bme18I GGWCC 2 cut(s) 620, 653
BmeT110I CYCGRG 2 cut(s) 9, 244
BmiI GGNNCC 8 cut(s) 75, 76, 163, 179, 384, 465, 745, 1068
BmrFI CCNGG 8 cut(s) 10, 11, 191, 343, 498, 806, 869, 1135
BmsI GCATC 3 cut(s) 217, 221, 303
BmtI GCTAGC 1 cut(s) 999
Bpu10I CCTNAGC 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 993
BpuMI CCSGG 6 cut(s) 10, 11, 191, 498, 869, 1135
BsaHI GRCGYC 2 cut(s) 383, 1067
BsaJI CCNNGG 7 cut(s) 9, 306, 312, 341, 372, 497, 804
BsaWI WCCGGW 1 cut(s) 412
Bsc4I CCNNNNNNNGG 7 cut(s) 170, 257, 318, 503, 810, 874, 1129
Bse118I RCCGGY 2 cut(s) 174, 425
Bse1I ACTGG 1 cut(s) 181
Bse3DI GCAATG 2 cut(s) 366, 670
BseBI CCWGG 2 cut(s) 343, 806
BseDI CCNNGG 7 cut(s) 9, 306, 312, 341, 372, 497, 804
BseGI GGATG 2 cut(s) 96, 232
BseLI CCNNNNNNNGG 7 cut(s) 170, 257, 318, 503, 810, 874, 1129
BseMI GCAATG 2 cut(s) 366, 670
BseMII CTCAG 3 cut(s) 62, 404, 1017
BseNI ACTGG 1 cut(s) 181
BseRI GAGGAG 1 cut(s) 1029
BseSI GKGCMC 3 cut(s) 77, 165, 982
BseX3I CGGCCG 1 cut(s) 420
BseYI CCCAGC 2 cut(s) 198, 829
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 423
BshNI GGYRCC 2 cut(s) 382, 1066
BsiEI CGRYCG 1 cut(s) 423
BsiHKAI GWGCWC 2 cut(s) 982, 1080
BsiHKCI CYCGRG 2 cut(s) 9, 244
BsiSI CCGG 8 cut(s) 10, 175, 191, 413, 426, 497, 869, 1134
BslFI GGGAC 2 cut(s) 787, 952
BslI CCNNNNNNNGG 7 cut(s) 170, 257, 318, 503, 810, 874, 1129
BsmFI GGGAC 2 cut(s) 787, 952
BsoBI CYCGRG 2 cut(s) 9, 244
Bsp120I GGGCCC 2 cut(s) 73, 161
Bsp1286I GDGCHC 6 cut(s) 77, 165, 239, 251, 982, 1080
Bsp143I GATC 4 cut(s) 153, 260, 463, 561
BspCNI CTCAG 3 cut(s) 61, 403, 1018
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 8 cut(s) 75, 76, 163, 179, 384, 465, 745, 1068
BspOI GCTAGC 1 cut(s) 999
BspPI GGATC 3 cut(s) 268, 458, 471
BspT107I GGYRCC 2 cut(s) 382, 1066
BsrDI GCAATG 2 cut(s) 366, 670
BsrFI RCCGGY 2 cut(s) 174, 425
BsrI ACTGG 1 cut(s) 181
BssAI RCCGGY 2 cut(s) 174, 425
BssECI CCNNGG 7 cut(s) 9, 306, 312, 341, 372, 497, 804
BssMI GATC 4 cut(s) 153, 260, 463, 561
BssNI GRCGYC 2 cut(s) 383, 1067
BssT1I CCWWGG 1 cut(s) 372
Bst2UI CCWGG 2 cut(s) 343, 806
Bst4CI ACNGT 3 cut(s) 703, 1085, 1149
Bst6I CTCTTC 1 cut(s) 307
BstACI GRCGYC 2 cut(s) 383, 1067
BstAPI GCANNNNNTGC 1 cut(s) 1111
BstDEI CTNAG 4 cut(s) 48, 390, 474, 1026
BstDSI CCRYGG 1 cut(s) 306
BstEII GGTNACC 1 cut(s) 770
BstF5I GGATG 2 cut(s) 96, 232
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 2 cut(s) 386, 1070
BstHHI GCGC 2 cut(s) 385, 1069
BstKTI GATC 4 cut(s) 156, 263, 466, 564
BstMBI GATC 4 cut(s) 153, 260, 463, 561
BstMCI CGRYCG 1 cut(s) 423
BstNI CCWGG 2 cut(s) 343, 806
BstNSI RCATGY 2 cut(s) 754, 853
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 8 cut(s) 8, 9, 189, 341, 496, 804, 867, 1133
BstSFI CTRYAG 1 cut(s) 1081
BstSLI GKGCMC 3 cut(s) 77, 165, 982
BstUI CGCG 1 cut(s) 149
BstX2I RGATCY 1 cut(s) 463
BstXI CCANNNNNNTGG 1 cut(s) 67
BstYI RGATCY 1 cut(s) 463
BstZI CGGCCG 1 cut(s) 420
BsuI GTATCC 1 cut(s) 647
BtgI CCRYGG 1 cut(s) 306
BtgZI GCGATG 1 cut(s) 164
BtsCI GGATG 2 cut(s) 96, 232
BtsIMutI CAGTG 1 cut(s) 831
CaiI CAGNNNCTG 1 cut(s) 815
CciNI GCGGCCGC 1 cut(s) 420
CfoI GCGC 2 cut(s) 385, 1069
Cfr10I RCCGGY 2 cut(s) 174, 425
Cfr9I CCCGGG 1 cut(s) 9
CseI GACGC 2 cut(s) 390, 530
Csp6I GTAC 5 cut(s) 184, 349, 699, 872, 1120
CviAII CATG 8 cut(s) 67, 232, 434, 454, 557, 751, 850, 1102
CviQI GTAC 5 cut(s) 184, 349, 699, 872, 1120
DdeI CTNAG 4 cut(s) 48, 390, 474, 1026
DinI GGCGCC 2 cut(s) 384, 1068
DpnI GATC 4 cut(s) 155, 262, 465, 563
DpnII GATC 4 cut(s) 153, 260, 463, 561
EaeI YGGCCR 1 cut(s) 420
EagI CGGCCG 1 cut(s) 420
Eam1104I CTCTTC 1 cut(s) 307
EarI CTCTTC 1 cut(s) 307
EciI GGCGGA 3 cut(s) 21, 133, 552
EclXI CGGCCG 1 cut(s) 420
Eco130I CCWWGG 1 cut(s) 372
Eco24I GRGCYC 4 cut(s) 77, 165, 239, 251
Eco47I GGWCC 2 cut(s) 620, 653
Eco52I CGGCCG 1 cut(s) 420
Eco88I CYCGRG 2 cut(s) 9, 244
Eco91I GGTNACC 1 cut(s) 770
EcoO109I RGGNCCY 2 cut(s) 74, 620
EcoO65I GGTNACC 1 cut(s) 770
EcoRII CCWGG 2 cut(s) 341, 804
EcoT14I CCWWGG 1 cut(s) 372
EcoT22I ATGCAT 1 cut(s) 1107
EcoT38I GRGCYC 4 cut(s) 77, 165, 239, 251
EgeI GGCGCC 2 cut(s) 384, 1068
EheI GGCGCC 2 cut(s) 384, 1068
ErhI CCWWGG 1 cut(s) 372
FaeI CATG 8 cut(s) 70, 235, 437, 457, 560, 754, 853, 1105
FaqI GGGAC 2 cut(s) 787, 952
FatI CATG 8 cut(s) 66, 231, 433, 453, 556, 750, 849, 1101
FauI CCCGC 3 cut(s) 152, 217, 310
FauNDI CATATG 1 cut(s) 709
FokI GGATG 2 cut(s) 83, 239
FriOI GRGCYC 4 cut(s) 77, 165, 239, 251
FspBI CTAG 5 cut(s) 240, 854, 915, 996, 1092
GlaI GCGC 2 cut(s) 384, 1068
GsaI CCCAGC 2 cut(s) 202, 833
HaeII RGCGCY 2 cut(s) 386, 1070
HapII CCGG 8 cut(s) 10, 175, 191, 413, 426, 497, 869, 1134
HgaI GACGC 2 cut(s) 390, 530
HhaI GCGC 2 cut(s) 385, 1069
Hin1I GRCGYC 2 cut(s) 383, 1067
Hin1II CATG 8 cut(s) 70, 235, 437, 457, 560, 754, 853, 1105
Hin6I GCGC 2 cut(s) 383, 1067
HinP1I GCGC 2 cut(s) 383, 1067
HinfI GANTC 4 cut(s) 5, 299, 440, 1028
HpaII CCGG 8 cut(s) 10, 175, 191, 413, 426, 497, 869, 1134
HphI GGTGA 1 cut(s) 442
Hpy166II GTNNAC 2 cut(s) 549, 980
Hpy188I TCNGA 4 cut(s) 463, 760, 817, 1036
Hpy188III TCNNGA 1 cut(s) 356
Hpy8I GTNNAC 2 cut(s) 549, 980
Hpy99I CGWCG 5 cut(s) 260, 362, 406, 463, 524
HpyAV CCTTC 6 cut(s) 262, 483, 589, 683, 896, 1011
HpyCH4III ACNGT 3 cut(s) 703, 1085, 1149
HpyCH4V TGCA 7 cut(s) 571, 647, 845, 860, 980, 1105, 1114
HpyF3I CTNAG 4 cut(s) 48, 390, 474, 1026
Hsp92I GRCGYC 2 cut(s) 383, 1067
Hsp92II CATG 8 cut(s) 70, 235, 437, 457, 560, 754, 853, 1105
HspAI GCGC 2 cut(s) 383, 1067
KasI GGCGCC 2 cut(s) 382, 1066
KroI GCCGGC 1 cut(s) 425
KroNI GCCGGC 1 cut(s) 427
Kzo9I GATC 4 cut(s) 153, 260, 463, 561
LmnI GCTCC 5 cut(s) 146, 415, 530, 749, 923
LweI GCATC 3 cut(s) 217, 221, 303
MaeI CTAG 5 cut(s) 240, 854, 915, 996, 1092
MaeIII GTNAC 5 cut(s) 448, 667, 703, 770, 1085
MalI GATC 4 cut(s) 155, 262, 465, 563
MboI GATC 4 cut(s) 153, 260, 463, 561
MboII GAAGA 6 cut(s) 294, 334, 337, 490, 520, 936
MflI RGATCY 1 cut(s) 463
MhlI GDGCHC 6 cut(s) 77, 165, 239, 251, 982, 1080
MluCI AATT 4 cut(s) 79, 166, 630, 879
Mly113I GGCGCC 2 cut(s) 383, 1067
MlyI GAGTC 3 cut(s) 293, 449, 1037
MmeI TCCRAC 1 cut(s) 441
Mph1103I ATGCAT 1 cut(s) 1107
MroNI GCCGGC 1 cut(s) 425
MseI TTAA 1 cut(s) 1059
MslI CAYNNNNRTG 1 cut(s) 68
MspA1I CMGCKG 3 cut(s) 223, 282, 992
MspI CCGG 8 cut(s) 10, 175, 191, 413, 426, 497, 869, 1134
MspR9I CCNGG 8 cut(s) 10, 11, 191, 343, 498, 806, 869, 1135
MvaI CCWGG 2 cut(s) 343, 806
MvnI CGCG 1 cut(s) 149
NaeI GCCGGC 1 cut(s) 427
NarI GGCGCC 2 cut(s) 383, 1067
NciI CCSGG 6 cut(s) 10, 11, 191, 498, 869, 1135
NdeI CATATG 1 cut(s) 709
NdeII GATC 4 cut(s) 153, 260, 463, 561
NgoMIV GCCGGC 1 cut(s) 425
NheI GCTAGC 1 cut(s) 995
NlaIII CATG 8 cut(s) 70, 235, 437, 457, 560, 754, 853, 1105
NlaIV GGNNCC 8 cut(s) 75, 76, 163, 179, 384, 465, 745, 1068
NmeAIII GCCGAG 1 cut(s) 293
NmuCI GTSAC 2 cut(s) 448, 703
NotI GCGGCCGC 1 cut(s) 420
NsiI ATGCAT 1 cut(s) 1107
NspI RCATGY 2 cut(s) 754, 853
PaeI GCATGC 1 cut(s) 853
PciI ACATGT 1 cut(s) 750
PcsI WCGNNNNNNNCGW 1 cut(s) 269
PdiI GCCGGC 1 cut(s) 427
PfeI GAWTC 1 cut(s) 5
PleI GAGTC 3 cut(s) 293, 448, 1036
PluTI GGCGCC 2 cut(s) 386, 1070
PpsI GAGTC 3 cut(s) 293, 448, 1036
PpuMI RGGWCCY 1 cut(s) 620
PscI ACATGT 1 cut(s) 750
Psp5II RGGWCCY 1 cut(s) 620
Psp6I CCWGG 2 cut(s) 341, 804
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 2 cut(s) 198, 829
PspGI CCWGG 2 cut(s) 341, 804
PspN4I GGNNCC 8 cut(s) 75, 76, 163, 179, 384, 465, 745, 1068
PspOMI GGGCCC 2 cut(s) 73, 161
PspPPI RGGWCCY 1 cut(s) 620
PstNI CAGNNNCTG 1 cut(s) 815
PsuI RGATCY 1 cut(s) 463
RsaI GTAC 5 cut(s) 185, 350, 700, 873, 1121
RsaNI GTAC 5 cut(s) 184, 349, 699, 872, 1120
RseI CAYNNNNRTG 1 cut(s) 68
SaqAI TTAA 1 cut(s) 1059
Sau3AI GATC 4 cut(s) 153, 260, 463, 561
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 3 cut(s) 293, 449, 1037
ScrFI CCNGG 8 cut(s) 10, 11, 191, 343, 498, 806, 869, 1135
SduI GDGCHC 6 cut(s) 77, 165, 239, 251, 982, 1080
SfaNI GCATC 3 cut(s) 217, 221, 303
SfcI CTRYAG 1 cut(s) 1081
SfoI GGCGCC 2 cut(s) 384, 1068
SinI GGWCC 2 cut(s) 620, 653
SmaI CCCGGG 1 cut(s) 11
SmiMI CAYNNNNRTG 1 cut(s) 68
SmlI CTYRAG 1 cut(s) 1008
SmoI CTYRAG 1 cut(s) 1008
SphI GCATGC 1 cut(s) 853
Sse9I AATT 4 cut(s) 79, 166, 630, 879
SspDI GGCGCC 2 cut(s) 382, 1066
SspMI CTAG 5 cut(s) 240, 854, 915, 996, 1092
StyD4I CCNGG 8 cut(s) 8, 9, 189, 341, 496, 804, 867, 1133
StyI CCWWGG 1 cut(s) 372
TaaI ACNGT 3 cut(s) 703, 1085, 1149
TaqI TCGA 6 cut(s) 215, 255, 297, 443, 519, 718
TasI AATT 4 cut(s) 79, 166, 630, 879
TatI WGTACW 2 cut(s) 183, 348
TauI GCSGC 9 cut(s) 37, 149, 208, 223, 279, 422, 425, 496, 995
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 1059
Tru9I TTAA 1 cut(s) 1059
TscAI CASTG 1 cut(s) 831
TseFI GTSAC 2 cut(s) 448, 703
Tsp45I GTSAC 2 cut(s) 448, 703
TspDTI ATGAA 1 cut(s) 617
TspGWI ACGGA 1 cut(s) 273
TspMI CCCGGG 1 cut(s) 9
TspRI CASTG 1 cut(s) 831
VneI GTGCAC 1 cut(s) 978
VpaK11BI GGWCC 2 cut(s) 620, 653
XceI RCATGY 2 cut(s) 754, 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XmaI CCCGGG 1 cut(s) 9
XspI CTAG 5 cut(s) 240, 854, 915, 996, 1092
ZrmI AGTACT 1 cut(s) 350
Zsp2I ATGCAT 1 cut(s) 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.