pycom16g24490

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013533
Physical Location & Seq
Forward (+)
25960037 .. 25965040
5004 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g24490.2

Sequence Viewer

Length: 1131 bp
ATGGATTCGCGGGAAGTTCCGCAGCAACAGCCGCCTCAGCAGCCGAACATGATGGTGGGCCCATCTTCATATCCCTCCTCTATGCCCAACGCCAACATAAACCCTAATTCGGGCTCCATGATGGGCGCCCCCAACCCGGGACGCTTCCCTTTCAACGCGGTGGCCCAGCAGCAGCAGCAGCAGCAGCAACAGCCCACTTCGAAACCGCAGATGGATTCTCTGAGCCCCAGCCCCTACGACGGGTCGTTGAGGCCGTGCGGCAGCGGCGGACCGTTCAACATCGACTCTTCTTCGGCTTCGGCTGCCAAGAAGAAGAGAGGGCGGCCCAGGAAGTACTCGCCTGACGGCAACATTGCCTTGGGATTGACGCCCACGCAGATTCCTTCTTCGGCTTCGGCCGCACCTGGGACCCATGGAGAGTCTAGTGGCACGATGTCTTCGGAGCCCCCAGCCAAGAAAAACCGGGGGAGGCCCCCAGGTTCGGGCAAGAAGCAGTTGGACGCATTGGGAGCTGGCGGAGTTGGATTCACACCTCATGTTATTATGGTGCAAGCCGGTGAGGTATCAGCAAAGGTTATGGCCTTTTCACAGCAGGGTCCGCGCACAGTTTGCATTCTCTCTGCAAATGGTGCCATCTGCAATGTTACCCTTCGCCAGCCAGCAATGTCTGGTGGTACTGTCACATATGAGGGTCGATATGAGATTATTTCCCTGTCAGGTTCCTACTTGTTTTCTGAAAATAATGGGAATCGCAGCAGAAGTGGTGGTTTGAGTGTATCCTTAGCAGGGTCTGATGGTCAAGTTTTGGGAGGTGGAGTTGCTGGCATGCTTGTGGCAGCATCACCAGTACAGGTGATTGTGGGTAGTTTCATTGCCGACGGGAAAAAATCCAACCCCAACTTGGTAAAGTCGGGAACTTCCTCTCCCCCAGCATCACAAATGTTGAACTTTGGTGCGCCAATGACAGCAGCCAGCCCGTCCTCTCAAGGAGGAGGGTCAAGCGAGTCGTCGGATGAGAATGGCAGCAGTCCTCTCAACAACAGCAACAGGGGACCTGTGCTCTACAGTAATGCAAATCAACCGATTCATAATATGCAGATGTATCAATTATGGGGTCAAGCTCAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

377

Amino Acids

38.62

Weight (kDa)

9.56

Isoelectric Point (pI)

62.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17950 AT5G46640
fragaria_vesca FvH4_5g22000
malus_domestica MD06G1033700.v1.1 MD16G1274800.v1.1
prunus_persica Prupe.5G037000_v2.0.a1
pyrus_communis pycom06g02780 pycom16g24490
rosa_chinensis RchiOBHm_Chr7g0209501
rosa_laevigata RLG00000003123
rosa_multiflora Rmu_sc0000243.1_g000005
rosa_roxburghii Rroxscaffold_3G00249310
rosa_rugosa Rorug07G0114000
rosa_samantha Rh7AG248000 Rh7BG242500 Rh7CG263500 Rh7DG254100
rosa_wichuraiana Rw7G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 125, 629
AccII CGCG 3 cut(s) 10, 158, 601
AcoI YGGCCR 1 cut(s) 396
AcyI GRCGYC 2 cut(s) 126, 368
AfaI GTAC 3 cut(s) 335, 676, 849
AgsI TTSAA 3 cut(s) 154, 277, 946
AjnI CCWGG 3 cut(s) 326, 403, 475
AloI GAACNNNNNNTCC 2 cut(s) 907, 939
AluBI AGCT 2 cut(s) 512, 1121
AluI AGCT 2 cut(s) 512, 1121
Alw21I GWGCWC 1 cut(s) 1062
AlwNI CAGNNNCTG 1 cut(s) 791
Ama87I CYCGRG 1 cut(s) 136
AoxI GGCC 7 cut(s) 58, 162, 251, 323, 396, 470, 579
ApaI GGGCCC 1 cut(s) 62
AspLEI GCGC 3 cut(s) 128, 603, 958
AspS9I GGNCC 9 cut(s) 58, 59, 163, 269, 324, 408, 471, 596, 1052
AsuC2I CCSGG 3 cut(s) 137, 138, 464
AsuHPI GGTGA 3 cut(s) 569, 834, 865
AsuII TTCGAA 1 cut(s) 200
AvaI CYCGRG 1 cut(s) 136
AvaII GGWCC 4 cut(s) 269, 408, 596, 1052
BaeGI GKGCMC 1 cut(s) 62
BanI GGYRCC 2 cut(s) 125, 629
BanII GRGCYC 4 cut(s) 62, 116, 227, 447
BbsI GAAGAC 1 cut(s) 429
Bbv12I GWGCWC 1 cut(s) 1062
BbvCI CCTCAGC 1 cut(s) 36
BccI CCATC 6 cut(s) 46, 70, 115, 205, 641, 788
BceAI ACGGC 2 cut(s) 238, 361
BciT130I CCWGG 3 cut(s) 328, 405, 477
BciVI GTATCC 1 cut(s) 787
BcnI CCSGG 3 cut(s) 137, 138, 464
BfaI CTAG 1 cut(s) 423
BfmI CTRYAG 1 cut(s) 1063
BfoI RGCGCY 1 cut(s) 129
BfuI GTATCC 1 cut(s) 787
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 6 cut(s) 137, 138, 328, 405, 464, 477
Bme18I GGWCC 4 cut(s) 269, 408, 596, 1052
BmeT110I CYCGRG 1 cut(s) 136
BmgT120I GGNCC 9 cut(s) 58, 59, 163, 269, 324, 408, 471, 596, 1052
BmrFI CCNGG 6 cut(s) 137, 138, 328, 405, 464, 477
BmsI GCATC 2 cut(s) 848, 941
BpiI GAAGAC 1 cut(s) 429
Bpu10I CCTNAGC 2 cut(s) 36, 781
Bpu14I TTCGAA 1 cut(s) 200
BpuEI CTTGAG 1 cut(s) 969
BpuMI CCSGG 3 cut(s) 137, 138, 464
BsaHI GRCGYC 2 cut(s) 126, 368
BsaJI CCNNGG 7 cut(s) 136, 326, 357, 404, 412, 463, 475
BsaXI ACNNNNNCTCC 4 cut(s) 801, 831, 907, 937
Bse118I RCCGGY 1 cut(s) 554
Bse1I ACTGG 1 cut(s) 845
Bse3DI GCAATG 4 cut(s) 351, 646, 669, 870
BseBI CCWGG 3 cut(s) 328, 405, 477
BseDI CCNNGG 7 cut(s) 136, 326, 357, 404, 412, 463, 475
BseGI GGATG 1 cut(s) 1018
BseMI GCAATG 4 cut(s) 351, 646, 669, 870
BseMII CTCAG 2 cut(s) 50, 212
BseNI ACTGG 1 cut(s) 845
BseRI GAGGAG 2 cut(s) 67, 1005
BseSI GKGCMC 1 cut(s) 62
BseX3I CGGCCG 1 cut(s) 396
BseYI CCCAGC 4 cut(s) 165, 227, 448, 928
Bsh1236I CGCG 3 cut(s) 10, 158, 601
Bsh1285I CGRYCG 1 cut(s) 399
BshFI GGCC 7 cut(s) 60, 164, 253, 325, 398, 472, 581
BshNI GGYRCC 2 cut(s) 125, 629
BsiEI CGRYCG 1 cut(s) 399
BsiHKAI GWGCWC 1 cut(s) 1062
BsiHKCI CYCGRG 1 cut(s) 136
BsiSI CCGG 3 cut(s) 137, 463, 555
BslFI GGGAC 3 cut(s) 153, 421, 1065
BsmFI GGGAC 3 cut(s) 153, 421, 1065
BsmI GAATGC 1 cut(s) 612
BsnI GGCC 7 cut(s) 60, 164, 253, 325, 398, 472, 581
BsoBI CYCGRG 1 cut(s) 136
Bsp119I TTCGAA 1 cut(s) 200
Bsp120I GGGCCC 1 cut(s) 58
Bsp1286I GDGCHC 5 cut(s) 62, 116, 227, 447, 1062
Bsp19I CCATGG 1 cut(s) 412
BspANI GGCC 7 cut(s) 60, 164, 253, 325, 398, 472, 581
BspCNI CTCAG 2 cut(s) 49, 213
BspFNI CGCG 3 cut(s) 10, 158, 601
BspT104I TTCGAA 1 cut(s) 200
BspT107I GGYRCC 2 cut(s) 125, 629
BsrDI GCAATG 4 cut(s) 351, 646, 669, 870
BsrFI RCCGGY 1 cut(s) 554
BsrI ACTGG 1 cut(s) 845
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 7 cut(s) 136, 326, 357, 404, 412, 463, 475
BssNI GRCGYC 2 cut(s) 126, 368
BssT1I CCWWGG 2 cut(s) 357, 412
Bst2UI CCWGG 3 cut(s) 328, 405, 477
Bst4CI ACNGT 4 cut(s) 273, 607, 679, 1067
Bst6I CTCTTC 2 cut(s) 292, 308
BstACI GRCGYC 2 cut(s) 126, 368
BstAPI GCANNNNNTGC 2 cut(s) 609, 629
BstBI TTCGAA 1 cut(s) 200
BstC8I GCNNGC 7 cut(s) 514, 552, 656, 660, 823, 827, 973
BstDEI CTNAG 3 cut(s) 36, 221, 781
BstDSI CCRYGG 1 cut(s) 412
BstF5I GGATG 1 cut(s) 1018
BstFNI CGCG 3 cut(s) 10, 158, 601
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 3 cut(s) 128, 603, 958
BstMCI CGRYCG 1 cut(s) 399
BstNI CCWGG 3 cut(s) 328, 405, 477
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 6 cut(s) 135, 136, 326, 403, 462, 475
BstSFI CTRYAG 1 cut(s) 1063
BstSLI GKGCMC 1 cut(s) 62
BstUI CGCG 3 cut(s) 10, 158, 601
BstV2I GAAGAC 1 cut(s) 429
BstZI CGGCCG 1 cut(s) 396
BsuI GTATCC 1 cut(s) 787
BsuRI GGCC 7 cut(s) 60, 164, 253, 325, 398, 472, 581
BtgI CCRYGG 1 cut(s) 412
BtsCI GGATG 1 cut(s) 1018
Cac8I GCNNGC 7 cut(s) 514, 552, 656, 660, 823, 827, 973
CaiI CAGNNNCTG 1 cut(s) 791
CfoI GCGC 3 cut(s) 128, 603, 958
Cfr10I RCCGGY 1 cut(s) 554
Cfr13I GGNCC 9 cut(s) 58, 59, 163, 269, 324, 408, 471, 596, 1052
Cfr9I CCCGGG 1 cut(s) 136
CpoI CGGWCCG 1 cut(s) 269
CseI GACGC 3 cut(s) 150, 376, 509
Csp6I GTAC 3 cut(s) 334, 675, 848
CspI CGGWCCG 1 cut(s) 269
CviAII CATG 5 cut(s) 49, 118, 413, 536, 826
CviQI GTAC 3 cut(s) 334, 675, 848
DdeI CTNAG 3 cut(s) 36, 221, 781
DinI GGCGCC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 396
EagI CGGCCG 1 cut(s) 396
Eam1104I CTCTTC 2 cut(s) 292, 308
EarI CTCTTC 2 cut(s) 292, 308
EciI GGCGGA 2 cut(s) 282, 531
EclXI CGGCCG 1 cut(s) 396
Eco130I CCWWGG 2 cut(s) 357, 412
Eco24I GRGCYC 4 cut(s) 62, 116, 227, 447
Eco47I GGWCC 4 cut(s) 269, 408, 596, 1052
Eco52I CGGCCG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 136
EcoO109I RGGNCCY 3 cut(s) 408, 471, 1052
EcoRII CCWGG 3 cut(s) 326, 403, 475
EcoT14I CCWWGG 2 cut(s) 357, 412
EcoT38I GRGCYC 4 cut(s) 62, 116, 227, 447
EgeI GGCGCC 1 cut(s) 127
EheI GGCGCC 1 cut(s) 127
ErhI CCWWGG 2 cut(s) 357, 412
FaeI CATG 5 cut(s) 52, 121, 416, 539, 829
FaqI GGGAC 3 cut(s) 153, 421, 1065
FatI CATG 5 cut(s) 48, 117, 412, 535, 825
FauI CCCGC 1 cut(s) 3
FauNDI CATATG 1 cut(s) 685
FokI GGATG 1 cut(s) 1025
FriOI GRGCYC 4 cut(s) 62, 116, 227, 447
FspBI CTAG 1 cut(s) 423
GlaI GCGC 3 cut(s) 127, 602, 957
GsaI CCCAGC 4 cut(s) 169, 231, 452, 932
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 7 cut(s) 60, 164, 253, 325, 398, 472, 581
HapII CCGG 3 cut(s) 137, 463, 555
HgaI GACGC 3 cut(s) 150, 376, 509
HhaI GCGC 3 cut(s) 128, 603, 958
Hin1I GRCGYC 2 cut(s) 126, 368
Hin1II CATG 5 cut(s) 52, 121, 416, 539, 829
Hin6I GCGC 3 cut(s) 126, 601, 956
HinP1I GCGC 3 cut(s) 126, 601, 956
HinfI GANTC 9 cut(s) 5, 215, 284, 379, 419, 525, 748, 1004, 1084
HpaII CCGG 3 cut(s) 137, 463, 555
HphI GGTGA 3 cut(s) 569, 834, 865
Hpy188I TCNGA 5 cut(s) 222, 442, 736, 793, 1012
Hpy188III TCNNGA 1 cut(s) 912
Hpy99I CGWCG 3 cut(s) 242, 881, 1012
HpyAV CCTTC 2 cut(s) 393, 659
HpyCH4III ACNGT 4 cut(s) 273, 607, 679, 1067
HpyCH4V TGCA 6 cut(s) 550, 612, 623, 639, 1073, 1096
HpyF3I CTNAG 3 cut(s) 36, 221, 781
Hsp92I GRCGYC 2 cut(s) 126, 368
Hsp92II CATG 5 cut(s) 52, 121, 416, 539, 829
HspAI GCGC 3 cut(s) 126, 601, 956
KasI GGCGCC 1 cut(s) 125
KflI GGGWCCC 1 cut(s) 408
LmnI GCTCC 3 cut(s) 119, 442, 509
LweI GCATC 2 cut(s) 848, 941
MaeI CTAG 1 cut(s) 423
MaeIII GTNAC 2 cut(s) 643, 679
MboII GAAGA 7 cut(s) 57, 279, 282, 322, 325, 378, 429
MhlI GDGCHC 5 cut(s) 62, 116, 227, 447, 1062
MluCI AATT 2 cut(s) 106, 1106
Mly113I GGCGCC 1 cut(s) 126
MlyI GAGTC 3 cut(s) 278, 428, 1013
MmeI TCCRAC 4 cut(s) 477, 502, 915, 990
MslI CAYNNNNRTG 2 cut(s) 53, 830
MspA1I CMGCKG 1 cut(s) 264
MspI CCGG 3 cut(s) 137, 463, 555
MspR9I CCNGG 6 cut(s) 137, 138, 328, 405, 464, 477
Mva1269I GAATGC 1 cut(s) 612
MvaI CCWGG 3 cut(s) 328, 405, 477
MvnI CGCG 3 cut(s) 10, 158, 601
NarI GGCGCC 1 cut(s) 126
NciI CCSGG 3 cut(s) 137, 138, 464
NcoI CCATGG 1 cut(s) 412
NdeI CATATG 1 cut(s) 685
NlaIII CATG 5 cut(s) 52, 121, 416, 539, 829
NmuCI GTSAC 1 cut(s) 679
NspI RCATGY 1 cut(s) 829
NspV TTCGAA 1 cut(s) 200
PaeI GCATGC 1 cut(s) 829
PcsI WCGNNNNNNNCGW 1 cut(s) 251
PctI GAATGC 1 cut(s) 612
PfeI GAWTC 6 cut(s) 5, 215, 379, 525, 748, 1084
PleI GAGTC 3 cut(s) 278, 427, 1012
PluTI GGCGCC 1 cut(s) 129
PpsI GAGTC 3 cut(s) 278, 427, 1012
PpuMI RGGWCCY 2 cut(s) 408, 1052
Psp5II RGGWCCY 2 cut(s) 408, 1052
Psp6I CCWGG 3 cut(s) 326, 403, 475
PspFI CCCAGC 4 cut(s) 165, 227, 448, 928
PspGI CCWGG 3 cut(s) 326, 403, 475
PspOMI GGGCCC 1 cut(s) 58
PspPI GGNCC 9 cut(s) 58, 59, 163, 269, 324, 408, 471, 596, 1052
PspPPI RGGWCCY 2 cut(s) 408, 1052
PstNI CAGNNNCTG 1 cut(s) 791
RsaI GTAC 3 cut(s) 335, 676, 849
RsaNI GTAC 3 cut(s) 334, 675, 848
RseI CAYNNNNRTG 2 cut(s) 53, 830
Rsr2I CGGWCCG 1 cut(s) 269
RsrII CGGWCCG 1 cut(s) 269
Sau96I GGNCC 9 cut(s) 58, 59, 163, 269, 324, 408, 471, 596, 1052
ScaI AGTACT 1 cut(s) 335
SchI GAGTC 3 cut(s) 278, 428, 1013
ScrFI CCNGG 6 cut(s) 137, 138, 328, 405, 464, 477
SduI GDGCHC 5 cut(s) 62, 116, 227, 447, 1062
SfaNI GCATC 2 cut(s) 848, 941
SfcI CTRYAG 1 cut(s) 1063
SfoI GGCGCC 1 cut(s) 127
SfuI TTCGAA 1 cut(s) 200
SinI GGWCC 4 cut(s) 269, 408, 596, 1052
SmaI CCCGGG 1 cut(s) 138
SmiMI CAYNNNNRTG 2 cut(s) 53, 830
SmlI CTYRAG 1 cut(s) 984
SmoI CTYRAG 1 cut(s) 984
SphI GCATGC 1 cut(s) 829
Sse9I AATT 2 cut(s) 106, 1106
SspDI GGCGCC 1 cut(s) 125
SspMI CTAG 1 cut(s) 423
StyD4I CCNGG 6 cut(s) 135, 136, 326, 403, 462, 475
StyI CCWWGG 2 cut(s) 357, 412
TaaI ACNGT 4 cut(s) 273, 607, 679, 1067
TaqI TCGA 3 cut(s) 200, 282, 694
TasI AATT 2 cut(s) 106, 1106
TatI WGTACW 2 cut(s) 333, 847
TauI GCSGC 5 cut(s) 34, 261, 267, 325, 401
TfiI GAWTC 6 cut(s) 5, 215, 379, 525, 748, 1084
TseFI GTSAC 1 cut(s) 679
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 3 cut(s) 57, 859, 1076
TspMI CCCGGG 1 cut(s) 136
VpaK11BI GGWCC 4 cut(s) 269, 408, 596, 1052
XceI RCATGY 1 cut(s) 829
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XmaI CCCGGG 1 cut(s) 136
XspI CTAG 1 cut(s) 423
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.