Rh7DG254100

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0013533
Physical Location & Seq
Reverse (-)
26482243 .. 26486752
4510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG254100.1

Sequence Viewer

Length: 747 bp
ATGGATTCCCGGGACGCCCCGCCGCAGCAGCCTCCGCCGCAGCAACCCAACATGATGGTGGGCCCCAATTCCTACCCTTCCCCCATGGCCAACCCCAACAACAACGCCACCGCCACCGCCAACAGCAACAACAACAACTCCTCGGCCGCGATGTTGGGCGGCGGCGGGCCGAATTCGGGTCGGTTCCAGTTCAACACGGTGGCCCAGCAGCAGCCCGCATCGAAGCCGCAGCCGCTCGATTCCATGAGCCCGAGCCCGTTCGACGGATCGCTGAGGCCGTGCGGCGGCGGCGGCGGCAGCGGAGGGTTTAGCATCGACTCTTCTACGGCCTCGGCGGGGAAGAAGAAGCGAGGGAGGCCCAGAAAGTACTCCCCTGATGGCAACATTGCATTGGGATTGGCGCCAACTCAGATTCCTGCCTCGGCTCCCGTTGCGGCCGCCGCCGGCCCCCACGGAGAATCGAGCGGCACCATGTCGTCGGATCCCCCTGCCAAGAAGAACAGGGGGCGCCCGCCGGGCTCCGGAAAGAAGCAGCTCGACGCTTTGGGAGCTGGTGGAGTTGGGTTCACGCCTCATGTCATTATGGTGCAAGCCGGCGAGGATATAGCATCAAAGGTTATGTCCTTTTCACAGCAAGGACCACGCACAGTTTGTATCCTTTCTGCAAATGGTCCAATCGGCAATGTTACCCTTCGCCAGCCATCAATGTCTGGTGGTACAGTCACATATGAGGTAGCTGTAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

248

Amino Acids

24.95

Weight (kDa)

9.82

Isoelectric Point (pI)

61.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 190 - 232 4.4e-08 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17950 AT5G46640
fragaria_vesca FvH4_5g22000
malus_domestica MD06G1033700.v1.1 MD16G1274800.v1.1
prunus_persica Prupe.5G037000_v2.0.a1
pyrus_communis pycom06g02780 pycom16g24490
rosa_chinensis RchiOBHm_Chr7g0209501
rosa_laevigata RLG00000003123
rosa_multiflora Rmu_sc0000243.1_g000005
rosa_roxburghii Rroxscaffold_3G00249310
rosa_rugosa Rorug07G0114000
rosa_samantha Rh7AG248000 Rh7BG242500 Rh7CG263500 Rh7DG254100
rosa_wichuraiana Rw7G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 400, 467, 507
AccBSI CCGCTC 2 cut(s) 235, 465
AccII CGCG 1 cut(s) 149
AccIII TCCGGA 1 cut(s) 521
AclWI GGATC 3 cut(s) 274, 476, 489
AcoI YGGCCR 3 cut(s) 87, 144, 435
AcsI RAATTY 1 cut(s) 172
AcyI GRCGYC 3 cut(s) 15, 401, 508
AfaI GTAC 2 cut(s) 368, 718
AfiI CCNNNNNNNGG 5 cut(s) 176, 263, 284, 336, 521
AgsI TTSAA 1 cut(s) 193
AluBI AGCT 3 cut(s) 535, 551, 737
AluI AGCT 3 cut(s) 535, 551, 737
AlwI GGATC 3 cut(s) 274, 476, 489
Ama87I CYCGRG 2 cut(s) 9, 250
Aor13HI TCCGGA 1 cut(s) 521
ApaI GGGCCC 1 cut(s) 65
ApeKI GCWGC 8 cut(s) 25, 28, 40, 208, 211, 229, 297, 532
ApoI RAATTY 1 cut(s) 172
ArsI GACNNNNNNTTYG 2 cut(s) 605, 637
AspLEI GCGC 2 cut(s) 403, 510
AspS9I GGNCC 8 cut(s) 61, 62, 167, 202, 357, 446, 638, 671
AsuC2I CCSGG 3 cut(s) 10, 11, 516
AvaI CYCGRG 2 cut(s) 9, 250
AvaII GGWCC 2 cut(s) 638, 671
BaeGI GKGCMC 1 cut(s) 65
BalI TGGCCA 1 cut(s) 89
BamHI GGATCC 1 cut(s) 481
BanI GGYRCC 3 cut(s) 400, 467, 507
BanII GRGCYC 4 cut(s) 65, 251, 257, 521
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 8 cut(s) 37, 40, 52, 220, 223, 241, 309, 544
BccI CCATC 3 cut(s) 49, 371, 709
BceAI ACGGC 2 cut(s) 262, 342
BciVI GTATCC 1 cut(s) 665
BcnI CCSGG 3 cut(s) 10, 11, 516
BfoI RGCGCY 2 cut(s) 404, 511
BfuI GTATCC 1 cut(s) 665
BglI GCCNNNNNGGC 1 cut(s) 516
BmcAI AGTACT 1 cut(s) 368
Bme1390I CCNGG 3 cut(s) 10, 11, 516
Bme18I GGWCC 2 cut(s) 638, 671
BmeT110I CYCGRG 2 cut(s) 9, 250
BmgT120I GGNCC 8 cut(s) 61, 62, 167, 202, 357, 446, 638, 671
BmrFI CCNGG 3 cut(s) 10, 11, 516
BmsI GCATC 3 cut(s) 227, 321, 617
Bpu10I CCTNAGC 1 cut(s) 272
BpuMI CCSGG 3 cut(s) 10, 11, 516
BsaHI GRCGYC 3 cut(s) 15, 401, 508
BsaJI CCNNGG 6 cut(s) 9, 84, 141, 330, 420, 451
BsaWI WCCGGW 1 cut(s) 521
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 263, 284, 336, 521
Bse118I RCCGGY 2 cut(s) 443, 593
Bse1I ACTGG 1 cut(s) 187
Bse3DI GCAATG 2 cut(s) 384, 688
BseAI TCCGGA 1 cut(s) 521
BseDI CCNNGG 6 cut(s) 9, 84, 141, 330, 420, 451
BseLI CCNNNNNNNGG 5 cut(s) 176, 263, 284, 336, 521
BseMI GCAATG 2 cut(s) 384, 688
BseMII CTCAG 2 cut(s) 263, 422
BseNI ACTGG 1 cut(s) 187
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 65
BseX3I CGGCCG 2 cut(s) 144, 435
BseXI GCAGC 8 cut(s) 37, 40, 52, 220, 223, 241, 309, 544
BseYI CCCAGC 1 cut(s) 204
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 2 cut(s) 147, 438
BshNI GGYRCC 3 cut(s) 400, 467, 507
BsiEI CGRYCG 2 cut(s) 147, 438
BsiHKCI CYCGRG 2 cut(s) 9, 250
BsiSI CCGG 5 cut(s) 10, 444, 515, 522, 594
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 5 cut(s) 176, 263, 284, 336, 521
BsmFI GGGAC 1 cut(s) 26
BsoBI CYCGRG 2 cut(s) 9, 250
Bsp120I GGGCCC 1 cut(s) 61
Bsp1286I GDGCHC 4 cut(s) 65, 251, 257, 521
Bsp13I TCCGGA 1 cut(s) 521
Bsp143I GATC 2 cut(s) 266, 481
Bsp19I CCATGG 1 cut(s) 84
BspCNI CTCAG 2 cut(s) 264, 421
BspEI TCCGGA 1 cut(s) 521
BspFNI CGCG 1 cut(s) 149
BspPI GGATC 3 cut(s) 274, 476, 489
BspT107I GGYRCC 3 cut(s) 400, 467, 507
BsrBI CCGCTC 2 cut(s) 235, 465
BsrDI GCAATG 2 cut(s) 384, 688
BsrFI RCCGGY 2 cut(s) 443, 593
BsrI ACTGG 1 cut(s) 187
BssAI RCCGGY 2 cut(s) 443, 593
BssECI CCNNGG 6 cut(s) 9, 84, 141, 330, 420, 451
BssMI GATC 2 cut(s) 266, 481
BssNI GRCGYC 3 cut(s) 15, 401, 508
BssT1I CCWWGG 1 cut(s) 84
Bst4CI ACNGT 3 cut(s) 199, 649, 721
Bst6I CTCTTC 1 cut(s) 325
BstACI GRCGYC 3 cut(s) 15, 401, 508
BstC8I GCNNGC 7 cut(s) 167, 216, 445, 512, 591, 595, 698
BstDEI CTNAG 2 cut(s) 272, 408
BstDSI CCRYGG 2 cut(s) 84, 451
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 2 cut(s) 404, 511
BstHHI GCGC 2 cut(s) 403, 510
BstKTI GATC 2 cut(s) 269, 484
BstMBI GATC 2 cut(s) 266, 481
BstMCI CGRYCG 2 cut(s) 147, 438
BstSCI CCNGG 3 cut(s) 8, 9, 514
BstSLI GKGCMC 1 cut(s) 65
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 8 cut(s) 37, 40, 52, 220, 223, 241, 309, 544
BstX2I RGATCY 1 cut(s) 481
BstXI CCANNNNNNTGG 1 cut(s) 55
BstYI RGATCY 1 cut(s) 481
BstZI CGGCCG 2 cut(s) 144, 435
BsuI GTATCC 1 cut(s) 665
BtgI CCRYGG 2 cut(s) 84, 451
BtgZI GCGATG 1 cut(s) 164
Cac8I GCNNGC 7 cut(s) 167, 216, 445, 512, 591, 595, 698
CciNI GCGGCCGC 1 cut(s) 435
CfoI GCGC 2 cut(s) 403, 510
Cfr10I RCCGGY 2 cut(s) 443, 593
Cfr13I GGNCC 8 cut(s) 61, 62, 167, 202, 357, 446, 638, 671
Cfr9I CCCGGG 1 cut(s) 9
CseI GACGC 2 cut(s) 23, 548
Csp6I GTAC 2 cut(s) 367, 717
CviAII CATG 5 cut(s) 52, 85, 244, 472, 575
CviQI GTAC 2 cut(s) 367, 717
DdeI CTNAG 2 cut(s) 272, 408
DinI GGCGCC 2 cut(s) 402, 509
DpnI GATC 2 cut(s) 268, 483
DpnII GATC 2 cut(s) 266, 481
EaeI YGGCCR 3 cut(s) 87, 144, 435
EagI CGGCCG 2 cut(s) 144, 435
Eam1104I CTCTTC 1 cut(s) 325
EarI CTCTTC 1 cut(s) 325
EciI GGCGGA 1 cut(s) 24
EclXI CGGCCG 2 cut(s) 144, 435
Eco130I CCWWGG 1 cut(s) 84
Eco24I GRGCYC 4 cut(s) 65, 251, 257, 521
Eco47I GGWCC 2 cut(s) 638, 671
Eco52I CGGCCG 2 cut(s) 144, 435
Eco88I CYCGRG 2 cut(s) 9, 250
EcoO109I RGGNCCY 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 84
EcoT38I GRGCYC 4 cut(s) 65, 251, 257, 521
EgeI GGCGCC 2 cut(s) 402, 509
EheI GGCGCC 2 cut(s) 402, 509
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 5 cut(s) 55, 88, 247, 475, 578
FaqI GGGAC 1 cut(s) 26
FatI CATG 5 cut(s) 51, 84, 243, 471, 574
FauI CCCGC 5 cut(s) 27, 158, 223, 328, 519
FauNDI CATATG 1 cut(s) 727
FriOI GRGCYC 4 cut(s) 65, 251, 257, 521
GlaI GCGC 2 cut(s) 402, 509
GsaI CCCAGC 1 cut(s) 208
HaeII RGCGCY 2 cut(s) 404, 511
HapII CCGG 5 cut(s) 10, 444, 515, 522, 594
HgaI GACGC 2 cut(s) 23, 548
HhaI GCGC 2 cut(s) 403, 510
Hin1I GRCGYC 3 cut(s) 15, 401, 508
Hin1II CATG 5 cut(s) 55, 88, 247, 475, 578
Hin6I GCGC 2 cut(s) 401, 508
HinP1I GCGC 2 cut(s) 401, 508
HinfI GANTC 5 cut(s) 5, 239, 317, 412, 458
HpaII CCGG 5 cut(s) 10, 444, 515, 522, 594
Hpy166II GTNNAC 1 cut(s) 567
Hpy188I TCNGA 2 cut(s) 411, 481
Hpy188III TCNNGA 1 cut(s) 522
Hpy8I GTNNAC 1 cut(s) 567
Hpy99I CGWCG 3 cut(s) 266, 481, 542
HpyAV CCTTC 2 cut(s) 87, 701
HpyCH4III ACNGT 3 cut(s) 199, 649, 721
HpyCH4V TGCA 3 cut(s) 389, 589, 665
HpyF3I CTNAG 2 cut(s) 272, 408
Hsp92I GRCGYC 3 cut(s) 15, 401, 508
Hsp92II CATG 5 cut(s) 55, 88, 247, 475, 578
HspAI GCGC 2 cut(s) 401, 508
KasI GGCGCC 2 cut(s) 400, 507
Kpn2I TCCGGA 1 cut(s) 521
KroI GCCGGC 2 cut(s) 443, 593
KroNI GCCGGC 2 cut(s) 445, 595
Kzo9I GATC 2 cut(s) 266, 481
LmnI GCTCC 3 cut(s) 430, 524, 548
Lsp1109I GCAGC 8 cut(s) 37, 40, 52, 220, 223, 241, 309, 544
LweI GCATC 3 cut(s) 227, 321, 617
MaeIII GTNAC 2 cut(s) 685, 721
MalI GATC 2 cut(s) 268, 483
MbiI CCGCTC 2 cut(s) 235, 465
MboI GATC 2 cut(s) 266, 481
MboII GAAGA 4 cut(s) 312, 352, 355, 508
MflI RGATCY 1 cut(s) 481
MhlI GDGCHC 4 cut(s) 65, 251, 257, 521
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 2 cut(s) 67, 172
MluNI TGGCCA 1 cut(s) 89
Mly113I GGCGCC 2 cut(s) 401, 508
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 1 cut(s) 459
Mox20I TGGCCA 1 cut(s) 89
MroI TCCGGA 1 cut(s) 521
MroNI GCCGGC 2 cut(s) 443, 593
MscI TGGCCA 1 cut(s) 89
MslI CAYNNNNRTG 2 cut(s) 56, 584
Msp20I TGGCCA 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 300
MspI CCGG 5 cut(s) 10, 444, 515, 522, 594
MspR9I CCNGG 3 cut(s) 10, 11, 516
MvnI CGCG 1 cut(s) 149
NaeI GCCGGC 2 cut(s) 445, 595
NarI GGCGCC 2 cut(s) 401, 508
NciI CCSGG 3 cut(s) 10, 11, 516
NcoI CCATGG 1 cut(s) 84
NdeI CATATG 1 cut(s) 727
NdeII GATC 2 cut(s) 266, 481
NgoMIV GCCGGC 2 cut(s) 443, 593
NlaIII CATG 5 cut(s) 55, 88, 247, 475, 578
NmeAIII GCCGAG 3 cut(s) 122, 311, 401
NmuCI GTSAC 1 cut(s) 721
NotI GCGGCCGC 1 cut(s) 435
PcsI WCGNNNNNNNCGW 1 cut(s) 275
PdiI GCCGGC 2 cut(s) 445, 595
PfeI GAWTC 4 cut(s) 5, 239, 412, 458
PleI GAGTC 1 cut(s) 311
PluTI GGCGCC 2 cut(s) 404, 511
PpsI GAGTC 1 cut(s) 311
PspFI CCCAGC 1 cut(s) 204
PspOMI GGGCCC 1 cut(s) 61
PspPI GGNCC 8 cut(s) 61, 62, 167, 202, 357, 446, 638, 671
PsuI RGATCY 1 cut(s) 481
RsaI GTAC 2 cut(s) 368, 718
RsaNI GTAC 2 cut(s) 367, 717
RseI CAYNNNNRTG 2 cut(s) 56, 584
Sau3AI GATC 2 cut(s) 266, 481
Sau96I GGNCC 8 cut(s) 61, 62, 167, 202, 357, 446, 638, 671
ScaI AGTACT 1 cut(s) 368
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 3 cut(s) 10, 11, 516
SduI GDGCHC 4 cut(s) 65, 251, 257, 521
SetI ASST 5 cut(s) 537, 553, 618, 735, 739
SfaNI GCATC 3 cut(s) 227, 321, 617
SfoI GGCGCC 2 cut(s) 402, 509
SinI GGWCC 2 cut(s) 638, 671
SmaI CCCGGG 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 56, 584
Sse9I AATT 2 cut(s) 67, 172
SspDI GGCGCC 2 cut(s) 400, 507
StyD4I CCNGG 3 cut(s) 8, 9, 514
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 3 cut(s) 199, 649, 721
TaqI TCGA 6 cut(s) 221, 237, 261, 315, 461, 537
TasI AATT 2 cut(s) 67, 172
TatI WGTACW 1 cut(s) 366
TfiI GAWTC 4 cut(s) 5, 239, 412, 458
TseFI GTSAC 1 cut(s) 721
TseI GCWGC 8 cut(s) 25, 28, 40, 208, 211, 229, 297, 532
Tsp45I GTSAC 1 cut(s) 721
TspGWI ACGGA 2 cut(s) 279, 468
TspMI CCCGGG 1 cut(s) 9
VpaK11BI GGWCC 2 cut(s) 638, 671
XapI RAATTY 1 cut(s) 172
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XmaI CCCGGG 1 cut(s) 9
ZrmI AGTACT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.