Rorug07G0114000

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0013533
Physical Location & Seq
Forward (+)
8911124 .. 8917147
6024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0114000.1

Sequence Viewer

Length: 2670 bp
ATGGGTGGGGTTACTTCATCCATGGCTGCAAAGCTTGCGTTTTTCCCACCAAACCCACCTTCCTACAAGGTCATTAAGGGCCAGGCAACTGGGCTCTTGCTCATCGACCCTTTTCCCCACCGTGAAAACGTCGACGTTTTGAAGTTTCAGACCCGCCGCGGCAATGAGATCGTGGCCGTTTATGTCCGCCACCCAATGGCCACCTCCACCGTTCTGTATTCTCATGGCAACGCTACTGATATTGGTCAGATGTATGAGCTCTTCATTGAATTGAGTATTCACCTGCGTGTCAATCTCATGGGGTATGATTACTCTGGATATGGGCAGTCATCAGGCAAGCCAAGTGAACATAATACTTATGCAGATATTGAAGCTGCATATAAGTGTCTCGAAGAGAGGTATGGTGCCAAGCAGGAGGACATAATCCTCTATGGTCAATCTGTCGGAAGTGGCCCTACTGTTGATCTTGCTGCTCGTTTGCCTCAACTGAGAGCAATTGTTCTGCATAGTCCGATATTATCAGGGTTAAGAGTTATGTATTCTGTGAAGCGCAGTTATTGGTTTGATATCTACAAGAACATTGATAAAATCCCACTGGTGAAATGTCCTGTGCTGGTAATACATGGAACATGTGATGAAGTTGTTGACTGCTCTCACGGCAAGCAACTTTGGCTACTGTGCCAAGAGAAATACGAACCTCTATGGCTCAAAGGCGGAAATCACTGCAATTTGGAACTCTATCCGGAATATATTAGACATCTCAACAAGTTCATATCCACTATTGAAAAAGCGCCTCCACGCAAGTTGAGTTCAAGGAAGAGCACAGACTGTGTCGAATGTCCTAGGCGTAGTGTTGAATATCCTAGGCACAGTGTTGATTACTCTGAGCTTCCGAGGAAGAGTACTGATAAGAGAGAAAAATCAAGGAGAAGCACCGATAGACGACCTGAAAAGCTGAGACTTAATGATTACAAGTTAAATAACAGTGACAAGCTAGAGAAATTTAGGATCTCTGTTGATCAGATAGAGAGGTCCCGAAGGAGCGTCGAATACCACCATGAGAGATCATCTAGGAGAAGCGTTGACCATCAGCTAGAAAAACCAAGGAGAAGTGTCGACTGGCTGGACAGAATTCGAGCTGGGTCGCAGGAGGAGGTAGCCAGGAAGTCGATTAAGCACGCGCTGAAGGCTCTAAGGAAGCGGCATTTGCTTGAAGAAGGTGCTCATGCTCCAGCGTATATCGCTCTCGCCAATCCAATTGCTCATCAGGGCTCTGAATGGAAGGAAAAGGCTGAGAATTTGGAACTGGAGCTTCAGCAATGCTATAAAGCTCAATCTCGGCTGTCGGAGCAGCTTGTGGTGGAAGTAGCAGAGTCCAGGTCTTCGAAAGCTTTACTTCAGGAGAAAGAAGCAGCGATTGCGGATCAGCAGAGTGAGCTCACACAAAAAAGGGATGAGTGCTCTCAGTTGAAGGCGGAGTTAGAAAAGAAGATTCAAGCTCTAGAGTTGGTTATGAGTGAGAACAATGAAATACGGGCGCAGCTAGAAGAAATGTCTGTTAGAGCTAAGAATGCTGAAGCTGAAAATAAGGTGTTAATTGACCGCTGGATGTTGGAAAAAATGAAGGATGCTGAACGCCTAAATGAGGCAAATGCACTATATGAAGAAATGCTTGAGCGGATCAAGGCCAGTGGTTTGCAAAAACTAGCCATGCAGCAAGTAGATGGTGTGGTCCGCCAAAGTGAAGAAGGTGCTGAGTACTTTGTGGAGTCAACCAATCCCACCACATGCAAGAACAGGATCAATGCCCATGACGGTGGCTGTGCTTCCATATTATTCGAGTACAACTCAGGCAAATTGATTTCTGGTGGACAGGATGGGACAATCAAAATGTGGGATACAAGTACAGGGGCTTTGAATAATACACTTCATGGTTGCATTGGTTCTGTTCTTGACCTTACCATTACCCATGATAATAGATCTATCATCGCAGCAAGCAGTTCGAACAAATTGTACGTATGGGATGCTAACTTGGGGAGAGTGCGCCATACTCTCACCGGCCACATGGATAAAGTTTGTGCTGTAGATGTGAGTAAGTTCTCAAGTCGTCACATTGTGAGTGCAGCCTACGATCGTACTATAAAAGTCTGGGATTTGCAAAAAGGTTACTGCACCAACACAATAATCTTCCCCAAACACTGCAATGCTCTTTGCTTCAGCATGGACGGGCAGACCATTTGCTCAGGCCATGTTGATGGGAATCTTCGGCTGTGGGATATTCAGAAAGGAAAGCTACTCAACGAAGTGGCTGCACACTCAAATGCTGTTACGTCTATATCTTTGTCGCGAAGTGGCAACATGGTATTGACCAGTGGGAGGGACAATGTGCATCACCTTTTCGATATGAGAACCCTAGAAGTTTGTGGCACATTGAGAGCCACCGGAAACAGAGTAGCATCTAATTGGAGCCGGTCCTGTATCAGTCCAGATGACAATTACGTTGCTGCTGGATCTGCCGATGGTGCTGTGTATATCTGGTCTATAGCCAAGGGTGACATTGTGAGCACTCTGAAGGAACACACTAATTCTGTCCTCTGCTGCTCATGGAGTGGACATGGAAAACCTCTAGCTTCTGCGGACAAGAATGGAGTAATTTGTACTTGGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

889

Amino Acids

100.01

Weight (kDa)

8.49

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 69 - 174 2.5e-07 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 71 - 171 5.2e-06 alpha/beta hydrolase fold
ATG16 PF08614 422 - 548 2.3e-11 Autophagy protein 16 (ATG16)
Beta-prop_WDR5 PF25175 593 - 729 1.5e-23 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 593 - 685 1.3e-11 THOC3 beta-propeller domain
WDR55 PF24796 595 - 803 2e-17 WDR55
WD40_CDC20-Fz PF24807 595 - 795 2.4e-21 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 596 - 721 4e-09 TEP-1 second beta-propeller
Beta-prop_SCAP PF24017 596 - 677 8.9e-06 SCAP Beta-propeller
Beta-prop_WDR3_2nd PF25172 597 - 675 2.6e-09 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 601 - 729 7.2e-21 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd PF25168 601 - 752 1.3e-15 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 601 - 695 2.2e-07 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 603 - 886 6.4e-29 WDHD1 first WD40 domain
WD40_Prp19 PF24814 607 - 816 4.5e-29 Prp19 WD40 domain
WD40_MABP1-WDR62_2nd PF24782 616 - 719 6.5e-07 MABP1/WDR62 second WD40 domain
Beta-prop_ACSF4 PF13570 654 - 886 5.9e-08 Beta-alanine-activating enzyme, beta-propeller
WD40_Gbeta PF25391 658 - 846 1.6e-18 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 659 - 887 9.7e-37 THOC3 beta-propeller domain
Beta-prop_WDR90_POC16_2nd PF23393 660 - 746 6.7e-06 WDR90/POC16, second beta-propeller
Beta-prop_WDR36-Utp21_1st PF25171 662 - 796 9.2e-11 WDR36/Utp21 first beta-propeller
Beta-prop_EML PF23409 663 - 801 9e-12 Echinoderm microtubule-associated protein first beta-propeller
EIF3I PF24805 663 - 746 3.5e-08 EIF3I
NBCH_WD40 PF20426 680 - 850 2.2e-07 Neurobeachin beta propeller domain
WD40 PF00400 680 - 718 3.4e-06 WD domain, G-beta repeat
Beta-prop_WDR3_2nd PF25172 683 - 801 2.3e-12 WDR3 second beta-propeller domain
Beta-prop_SCAP PF24017 698 - 856 6.4e-06 SCAP Beta-propeller
Beta-prop_EML_2 PF23414 702 - 814 4.6e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR90_POC16_2nd PF23393 702 - 821 1.1e-09 WDR90/POC16, second beta-propeller
WDR55 PF24796 731 - 888 1.9e-13 WDR55
EIF3I PF24805 734 - 835 1e-11 EIF3I
Beta-prop_WDR3_1st PF25173 734 - 820 6.7e-12 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 735 - 818 2.6e-15 WDR5 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 736 - 808 3.3e-07 CAF1B/HIR1 beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 736 - 803 8.5e-07 MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_2nd PF25168 744 - 881 1.5e-08 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 752 - 879 1.4e-06 G protein beta WD-40 repeat protein
Beta-prop_TEP1_2nd PF25047 768 - 853 3.7e-06 TEP-1 second beta-propeller
WDR90_beta-prop_4th PF23342 822 - 886 3.2e-06 WDR90, 4th beta-propeller
Beta-prop_WDR3_1st PF25173 825 - 888 8e-10 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 826 - 886 1.3e-06 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 826 - 888 7.3e-10 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 827 - 888 2.2e-06 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 828 - 888 3.1e-06 Prp19 WD40 domain
Beta-prop_IFT140_1st PF23383 829 - 888 1.4e-06 IFT140 first beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17950 AT5G46640
fragaria_vesca FvH4_5g22000
malus_domestica MD06G1033700.v1.1 MD16G1274800.v1.1
prunus_persica Prupe.5G037000_v2.0.a1
pyrus_communis pycom06g02780 pycom16g24490
rosa_chinensis RchiOBHm_Chr7g0209501
rosa_laevigata RLG00000003123
rosa_multiflora Rmu_sc0000243.1_g000005
rosa_roxburghii Rroxscaffold_3G00249310
rosa_rugosa Rorug07G0114000
rosa_samantha Rh7AG248000 Rh7BG242500 Rh7CG263500 Rh7DG254100
rosa_wichuraiana Rw7G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 291
Acc36I ACCTGC 1 cut(s) 291
AccB1I GGYRCC 1 cut(s) 404
AccB7I CCANNNNNTGG 1 cut(s) 196
AccBSI CCGCTC 1 cut(s) 1678
AccI GTMKAC 2 cut(s) 132, 1116
AccII CGCG 3 cut(s) 159, 1181, 2347
AccIII TCCGGA 1 cut(s) 742
AclWI GGATC 5 cut(s) 1016, 1431, 1688, 1808, 2518
AcoI YGGCCR 3 cut(s) 174, 198, 2059
AcsI RAATTY 3 cut(s) 1001, 1131, 1297
AcuI CTGAAG 6 cut(s) 1205, 1298, 1382, 1596, 2200, 2591
AdeI CACNNNGTG 1 cut(s) 2116
AfaI GTAC 7 cut(s) 904, 1760, 1844, 1906, 2015, 2137, 2659
AfiI CCNNNNNNNGG 3 cut(s) 196, 1645, 2376
AflIII ACRYGT 1 cut(s) 629
AjiI CACGTC 1 cut(s) 2667
AjnI CCWGG 3 cut(s) 81, 1160, 1376
AjuI GAANNNNNNNTTGG 2 cut(s) 43, 75
AleI CACNNNNGTG 1 cut(s) 285
Alw21I GWGCWC 6 cut(s) 261, 824, 1225, 1440, 1463, 2567
Alw26I GTCTC 2 cut(s) 392, 952
AlwI GGATC 5 cut(s) 1016, 1431, 1688, 1808, 2518
Aor13HI TCCGGA 1 cut(s) 742
AoxI GGCC 7 cut(s) 79, 174, 198, 451, 1686, 2059, 2245
ApoI RAATTY 3 cut(s) 1001, 1131, 1297
AspA2I CCTAGG 2 cut(s) 842, 863
AspLEI GCGC 5 cut(s) 552, 793, 1183, 1540, 2046
AspS9I GGNCC 5 cut(s) 79, 452, 1032, 1732, 2472
AsuHPI GGTGA 5 cut(s) 272, 610, 2047, 2384, 2564
AsuII TTCGAA 2 cut(s) 1385, 2003
AvaII GGWCC 3 cut(s) 1032, 1732, 2472
AvrII CCTAGG 2 cut(s) 842, 863
BalI TGGCCA 1 cut(s) 200
BanI GGYRCC 1 cut(s) 404
BanII GRGCYC 4 cut(s) 96, 261, 1274, 1440
BarI GAAGNNNNNNTAC 4 cut(s) 245, 277, 439, 471
BbsI GAAGAC 1 cut(s) 1374
Bbv12I GWGCWC 6 cut(s) 261, 824, 1225, 1440, 1463, 2567
BccI CCATC 5 cut(s) 1095, 1718, 1871, 2249, 2513
BceAI ACGGC 2 cut(s) 161, 673
BcgI CGANNNNNNTGC 6 cut(s) 151, 185, 1983, 2017, 2291, 2325
BciT130I CCWGG 3 cut(s) 83, 1162, 1378
BciVI GTATCC 1 cut(s) 1891
BclI TGATCA 1 cut(s) 1018
BcoDI GTCTC 2 cut(s) 392, 952
BfmI CTRYAG 2 cut(s) 2082, 2541
BfoI RGCGCY 1 cut(s) 794
BfuAI ACCTGC 1 cut(s) 291
BfuI GTATCC 1 cut(s) 1891
BglII AGATCT 1 cut(s) 1979
BlnI CCTAGG 2 cut(s) 842, 863
BmcAI AGTACT 2 cut(s) 904, 1760
Bme1390I CCNGG 3 cut(s) 83, 1162, 1378
Bme18I GGWCC 3 cut(s) 1032, 1732, 2472
BmgBI CACGTC 1 cut(s) 2667
BmgT120I GGNCC 5 cut(s) 79, 452, 1032, 1732, 2472
BmiI GGNNCC 3 cut(s) 406, 1034, 2468
BmrFI CCNGG 3 cut(s) 83, 1162, 1378
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 4 cut(s) 1618, 2014, 2398, 2465
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 1374
BplI GAGNNNNNCTC 2 cut(s) 1832, 1864
BpmI CTGGAG 2 cut(s) 1215, 1328
Bpu10I CCTNAGC 1 cut(s) 2242
Bpu14I TTCGAA 2 cut(s) 1385, 2003
BpuEI CTTGAG 2 cut(s) 1694, 2086
BsaAI YACGTR 1 cut(s) 2017
BsaBI GATNNNNATC 1 cut(s) 2259
BsaJI CCNNGG 7 cut(s) 21, 157, 842, 863, 893, 1103, 2547
BsaWI WCCGGW 2 cut(s) 742, 2441
Bsc4I CCNNNNNNNGG 3 cut(s) 196, 1645, 2376
Bse118I RCCGGY 2 cut(s) 2057, 2469
Bse1I ACTGG 6 cut(s) 94, 600, 1124, 1311, 1689, 2370
Bse3DI GCAATG 3 cut(s) 169, 1325, 2209
Bse8I GATNNNNATC 1 cut(s) 2259
BseAI TCCGGA 1 cut(s) 742
BseBI CCWGG 3 cut(s) 83, 1162, 1378
BseDI CCNNGG 7 cut(s) 21, 157, 842, 863, 893, 1103, 2547
BseGI GGATG 6 cut(s) 17, 1459, 1614, 1633, 1882, 2029
BseJI GATNNNNATC 1 cut(s) 2259
BseLI CCNNNNNNNGG 3 cut(s) 196, 1645, 2376
BseMI GCAATG 3 cut(s) 169, 1325, 2209
BseMII CTCAG 8 cut(s) 479, 876, 947, 1284, 1478, 1746, 1863, 2256
BseNI ACTGG 6 cut(s) 94, 600, 1124, 1311, 1689, 2370
BseRI GAGGAG 1 cut(s) 1166
BseYI CCCAGC 1 cut(s) 1139
BsgI GTGCAG 3 cut(s) 2142, 2155, 2295
Bsh1236I CGCG 3 cut(s) 159, 1181, 2347
Bsh1285I CGRYCG 1 cut(s) 2134
BshFI GGCC 7 cut(s) 81, 176, 200, 453, 1688, 2061, 2247
BshNI GGYRCC 1 cut(s) 404
BsiEI CGRYCG 1 cut(s) 2134
BsiHKAI GWGCWC 6 cut(s) 261, 824, 1225, 1440, 1463, 2567
BsiSI CCGG 4 cut(s) 743, 2058, 2442, 2470
BslFI GGGAC 3 cut(s) 1018, 1894, 2393
BslI CCNNNNNNNGG 3 cut(s) 196, 1645, 2376
BsmAI GTCTC 2 cut(s) 392, 952
BsmFI GGGAC 3 cut(s) 1018, 1894, 2393
BsmI GAATGC 1 cut(s) 1576
BsnI GGCC 7 cut(s) 81, 176, 200, 453, 1688, 2061, 2247
Bsp119I TTCGAA 2 cut(s) 1385, 2003
Bsp1286I GDGCHC 8 cut(s) 96, 261, 824, 1225, 1274, 1440, 1463, 2567
Bsp13I TCCGGA 1 cut(s) 742
Bsp19I CCATGG 1 cut(s) 21
Bsp68I TCGCGA 1 cut(s) 2347
BspANI GGCC 7 cut(s) 81, 176, 200, 453, 1688, 2061, 2247
BspCNI CTCAG 8 cut(s) 480, 877, 948, 1285, 1477, 1747, 1862, 2255
BspEI TCCGGA 1 cut(s) 742
BspFNI CGCG 3 cut(s) 159, 1181, 2347
BspLI GGNNCC 3 cut(s) 406, 1034, 2468
BspMI ACCTGC 1 cut(s) 291
BspPI GGATC 5 cut(s) 1016, 1431, 1688, 1808, 2518
BspQI GCTCTTC 2 cut(s) 266, 812
BspT104I TTCGAA 2 cut(s) 1385, 2003
BspT107I GGYRCC 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 1678
BsrDI GCAATG 3 cut(s) 169, 1325, 2209
BsrFI RCCGGY 2 cut(s) 2057, 2469
BsrI ACTGG 6 cut(s) 94, 600, 1124, 1311, 1689, 2370
BssAI RCCGGY 2 cut(s) 2057, 2469
BssECI CCNNGG 7 cut(s) 21, 157, 842, 863, 893, 1103, 2547
BssT1I CCWWGG 5 cut(s) 21, 842, 863, 1103, 2547
Bst2UI CCWGG 3 cut(s) 83, 1162, 1378
Bst4CI ACNGT 8 cut(s) 122, 211, 460, 678, 830, 872, 986, 1817
Bst6I CTCTTC 4 cut(s) 266, 387, 812, 893
BstAPI GCANNNNNTGC 2 cut(s) 35, 1418
BstBAI YACGTR 1 cut(s) 2017
BstBI TTCGAA 2 cut(s) 1385, 2003
BstC8I GCNNGC 5 cut(s) 36, 338, 662, 1179, 1996
BstDSI CCRYGG 2 cut(s) 21, 157
BstENI CCTNNNNNAGG 1 cut(s) 1643
BstF5I GGATG 6 cut(s) 17, 1459, 1614, 1633, 1882, 2029
BstFNI CGCG 3 cut(s) 159, 1181, 2347
BstH2I RGCGCY 1 cut(s) 794
BstHHI GCGC 5 cut(s) 552, 793, 1183, 1540, 2046
BstMAI GTCTC 2 cut(s) 392, 952
BstMCI CGRYCG 1 cut(s) 2134
BstNI CCWGG 3 cut(s) 83, 1162, 1378
BstNSI RCATGY 2 cut(s) 633, 1791
BstSCI CCNGG 3 cut(s) 81, 1160, 1376
BstSFI CTRYAG 2 cut(s) 2082, 2541
BstSNI TACGTA 1 cut(s) 2017
BstUI CGCG 3 cut(s) 159, 1181, 2347
BstV2I GAAGAC 1 cut(s) 1374
BstX2I RGATCY 3 cut(s) 1008, 1979, 2510
BstXI CCANNNNNNTGG 3 cut(s) 89, 1817, 2255
BstYI RGATCY 3 cut(s) 1008, 1979, 2510
BsuI GTATCC 1 cut(s) 1891
BsuRI GGCC 7 cut(s) 81, 176, 200, 453, 1688, 2061, 2247
BtgI CCRYGG 2 cut(s) 21, 157
BtgZI GCGATG 1 cut(s) 1972
BtrI CACGTC 1 cut(s) 2667
BtsCI GGATG 6 cut(s) 17, 1459, 1614, 1633, 1882, 2029
BtsI GCAGTG 2 cut(s) 721, 2197
BtsIMutI CAGTG 7 cut(s) 593, 721, 877, 991, 1696, 2197, 2377
BtuMI TCGCGA 1 cut(s) 2347
BveI ACCTGC 1 cut(s) 291
Cac8I GCNNGC 5 cut(s) 36, 338, 662, 1179, 1996
CfoI GCGC 5 cut(s) 552, 793, 1183, 1540, 2046
Cfr10I RCCGGY 2 cut(s) 2057, 2469
Cfr13I GGNCC 5 cut(s) 79, 452, 1032, 1732, 2472
Cfr42I CCGCGG 1 cut(s) 160
CseI GACGC 1 cut(s) 1033
Csp6I GTAC 7 cut(s) 903, 1759, 1843, 1905, 2014, 2136, 2658
CspCI CAANNNNNGTGG 4 cut(s) 786, 821, 1672, 1707
CviQI GTAC 7 cut(s) 903, 1759, 1843, 1905, 2014, 2136, 2658
DraIII CACNNNGTG 1 cut(s) 2116
EaeI YGGCCR 3 cut(s) 174, 198, 2059
Eam1104I CTCTTC 4 cut(s) 266, 387, 812, 893
EarI CTCTTC 4 cut(s) 266, 387, 812, 893
EciI GGCGGA 4 cut(s) 176, 729, 1490, 1724
Ecl136II GAGCTC 2 cut(s) 259, 1438
Eco105I TACGTA 1 cut(s) 2017
Eco130I CCWWGG 5 cut(s) 21, 842, 863, 1103, 2547
Eco24I GRGCYC 4 cut(s) 96, 261, 1274, 1440
Eco32I GATATC 1 cut(s) 568
Eco47I GGWCC 3 cut(s) 1032, 1732, 2472
Eco53kI GAGCTC 2 cut(s) 259, 1438
Eco57I CTGAAG 6 cut(s) 1205, 1298, 1382, 1596, 2200, 2591
EcoICRI GAGCTC 2 cut(s) 259, 1438
EcoNI CCTNNNNNAGG 1 cut(s) 1643
EcoO109I RGGNCCY 1 cut(s) 1032
EcoRI GAATTC 1 cut(s) 1131
EcoRII CCWGG 3 cut(s) 81, 1160, 1376
EcoRV GATATC 1 cut(s) 568
EcoT14I CCWWGG 5 cut(s) 21, 842, 863, 1103, 2547
EcoT38I GRGCYC 4 cut(s) 96, 261, 1274, 1440
ErhI CCWWGG 5 cut(s) 21, 842, 863, 1103, 2547
FalI AAGNNNNNCTT 4 cut(s) 1380, 1412, 1656, 1688
FaqI GGGAC 3 cut(s) 1018, 1894, 2393
FauI CCCGC 1 cut(s) 161
FbaI TGATCA 1 cut(s) 1018
FblI GTMKAC 2 cut(s) 132, 1116
FokI GGATG 6 cut(s) 4, 1466, 1621, 1640, 1889, 2036
FriOI GRGCYC 4 cut(s) 96, 261, 1274, 1440
GlaI GCGC 5 cut(s) 551, 792, 1182, 1539, 2045
GsaI CCCAGC 1 cut(s) 1143
GsuI CTGGAG 2 cut(s) 1215, 1328
HaeII RGCGCY 1 cut(s) 794
HaeIII GGCC 7 cut(s) 81, 176, 200, 453, 1688, 2061, 2247
HapII CCGG 4 cut(s) 743, 2058, 2442, 2470
HgaI GACGC 1 cut(s) 1033
HhaI GCGC 5 cut(s) 552, 793, 1183, 1540, 2046
Hin6I GCGC 5 cut(s) 550, 791, 1181, 1538, 2044
HinP1I GCGC 5 cut(s) 550, 791, 1181, 1538, 2044
HincII GTYRAC 5 cut(s) 133, 646, 1084, 1117, 1773
HindII GTYRAC 5 cut(s) 133, 646, 1084, 1117, 1773
HindIII AAGCTT 2 cut(s) 32, 1389
HinfI GANTC 4 cut(s) 1373, 1492, 1769, 2260
HpaII CCGG 4 cut(s) 743, 2058, 2442, 2470
HphI GGTGA 5 cut(s) 272, 610, 2047, 2384, 2564
Hpy166II GTNNAC 8 cut(s) 133, 347, 646, 1084, 1117, 1773, 1871, 2612
Hpy188III TCNNGA 9 cut(s) 315, 389, 743, 1035, 1400, 1502, 1952, 2346, 2486
Hpy8I GTNNAC 8 cut(s) 133, 347, 646, 1084, 1117, 1773, 1871, 2612
Hpy99I CGWCG 3 cut(s) 134, 137, 1049
HpyAV CCTTC 9 cut(s) 69, 1032, 1180, 1211, 1276, 1465, 1618, 1742, 2566
HpyCH4III ACNGT 8 cut(s) 122, 211, 460, 678, 830, 872, 986, 1817
HpyCH4IV ACGT 6 cut(s) 129, 135, 2016, 2330, 2499, 2666
HpySE526I ACGT 6 cut(s) 129, 135, 2016, 2330, 2499, 2666
HspAI GCGC 5 cut(s) 550, 791, 1181, 1538, 2044
Kpn2I TCCGGA 1 cut(s) 742
Ksp22I TGATCA 1 cut(s) 1018
KspI CCGCGG 1 cut(s) 160
LguI GCTCTTC 2 cut(s) 266, 812
LmnI GCTCC 5 cut(s) 1041, 1234, 1309, 1348, 2466
LweI GCATC 4 cut(s) 1618, 2014, 2398, 2465
MaeII ACGT 6 cut(s) 129, 135, 2016, 2330, 2499, 2666
MaeIII GTNAC 6 cut(s) 10, 986, 2108, 2165, 2326, 2552
MbiI CCGCTC 1 cut(s) 1678
MfeI CAATTG 2 cut(s) 495, 1257
MflI RGATCY 3 cut(s) 1008, 1979, 2510
MhlI GDGCHC 8 cut(s) 96, 261, 824, 1225, 1274, 1440, 1463, 2567
MlsI TGGCCA 1 cut(s) 200
MluNI TGGCCA 1 cut(s) 200
MlyI GAGTC 2 cut(s) 1382, 1778
MmeI TCCRAC 3 cut(s) 424, 1326, 1593
Mox20I TGGCCA 1 cut(s) 200
MroI TCCGGA 1 cut(s) 742
MscI TGGCCA 1 cut(s) 200
MseI TTAA 6 cut(s) 75, 527, 963, 977, 1173, 1595
MslI CAYNNNNRTG 6 cut(s) 285, 382, 1815, 2202, 2253, 2319
Msp20I TGGCCA 1 cut(s) 200
MspA1I CMGCKG 2 cut(s) 159, 1605
MspI CCGG 4 cut(s) 743, 2058, 2442, 2470
MspR9I CCNGG 3 cut(s) 83, 1162, 1378
MunI CAATTG 2 cut(s) 495, 1257
Mva1269I GAATGC 1 cut(s) 1576
MvaI CCWGG 3 cut(s) 83, 1162, 1378
MvnI CGCG 3 cut(s) 159, 1181, 2347
NcoI CCATGG 1 cut(s) 21
NlaIV GGNNCC 3 cut(s) 406, 1034, 2468
NmeAIII GCCGAG 1 cut(s) 1318
NmuCI GTSAC 3 cut(s) 986, 2108, 2552
NruI TCGCGA 1 cut(s) 2347
NspI RCATGY 2 cut(s) 633, 1791
NspV TTCGAA 2 cut(s) 1385, 2003
OliI CACNNNNGTG 1 cut(s) 285
PaqCI CACCTGC 1 cut(s) 291
PciI ACATGT 1 cut(s) 629
PciSI GCTCTTC 2 cut(s) 266, 812
PctI GAATGC 1 cut(s) 1576
PfeI GAWTC 2 cut(s) 1492, 2260
PflFI GACNNNGTC 1 cut(s) 830
PflMI CCANNNNNTGG 1 cut(s) 196
Ple19I CGATCG 1 cut(s) 2134
PleI GAGTC 2 cut(s) 1381, 1777
PpsI GAGTC 2 cut(s) 1381, 1777
Ppu21I YACGTR 1 cut(s) 2017
PpuMI RGGWCCY 1 cut(s) 1032
PscI ACATGT 1 cut(s) 629
Psp124BI GAGCTC 2 cut(s) 261, 1440
Psp5II RGGWCCY 1 cut(s) 1032
Psp6I CCWGG 3 cut(s) 81, 1160, 1376
PspFI CCCAGC 1 cut(s) 1139
PspGI CCWGG 3 cut(s) 81, 1160, 1376
PspN4I GGNNCC 3 cut(s) 406, 1034, 2468
PspPI GGNCC 5 cut(s) 79, 452, 1032, 1732, 2472
PspPPI RGGWCCY 1 cut(s) 1032
PsrI GAACNNNNNNTAC 2 cut(s) 1997, 2029
PsuI RGATCY 3 cut(s) 1008, 1979, 2510
PsyI GACNNNGTC 1 cut(s) 830
PvuI CGATCG 1 cut(s) 2134
RruI TCGCGA 1 cut(s) 2347
RsaI GTAC 7 cut(s) 904, 1760, 1844, 1906, 2015, 2137, 2659
RsaNI GTAC 7 cut(s) 903, 1759, 1843, 1905, 2014, 2136, 2658
RseI CAYNNNNRTG 6 cut(s) 285, 382, 1815, 2202, 2253, 2319
SacI GAGCTC 2 cut(s) 261, 1440
SacII CCGCGG 1 cut(s) 160
SalI GTCGAC 2 cut(s) 131, 1115
SapI GCTCTTC 2 cut(s) 266, 812
SaqAI TTAA 6 cut(s) 75, 527, 963, 977, 1173, 1595
Sau96I GGNCC 5 cut(s) 79, 452, 1032, 1732, 2472
ScaI AGTACT 2 cut(s) 904, 1760
SchI GAGTC 2 cut(s) 1382, 1778
ScrFI CCNGG 3 cut(s) 83, 1162, 1378
SduI GDGCHC 8 cut(s) 96, 261, 824, 1225, 1274, 1440, 1463, 2567
SfaNI GCATC 4 cut(s) 1618, 2014, 2398, 2465
SfcI CTRYAG 2 cut(s) 2082, 2541
Sfr303I CCGCGG 1 cut(s) 160
SfuI TTCGAA 2 cut(s) 1385, 2003
SgrBI CCGCGG 1 cut(s) 160
SgrDI CGTCGACG 1 cut(s) 131
SinI GGWCC 3 cut(s) 1032, 1732, 2472
SmiMI CAYNNNNRTG 6 cut(s) 285, 382, 1815, 2202, 2253, 2319
SmlI CTYRAG 2 cut(s) 1673, 2101
SmoI CTYRAG 2 cut(s) 1673, 2101
SnaBI TACGTA 1 cut(s) 2017
SstI GAGCTC 2 cut(s) 261, 1440
StyD4I CCNGG 3 cut(s) 81, 1160, 1376
StyI CCWWGG 5 cut(s) 21, 842, 863, 1103, 2547
TaaI ACNGT 8 cut(s) 122, 211, 460, 678, 830, 872, 986, 1817
TaiI ACGT 6 cut(s) 132, 138, 2019, 2333, 2502, 2669
TatI WGTACW 5 cut(s) 902, 1758, 1842, 1904, 2657
TauI GCSGC 3 cut(s) 159, 162, 1204
TfiI GAWTC 2 cut(s) 1492, 2260
Tru1I TTAA 6 cut(s) 75, 527, 963, 977, 1173, 1595
Tru9I TTAA 6 cut(s) 75, 527, 963, 977, 1173, 1595
TscAI CASTG 7 cut(s) 600, 728, 877, 991, 1696, 2204, 2377
TseFI GTSAC 3 cut(s) 986, 2108, 2552
Tsp45I GTSAC 3 cut(s) 986, 2108, 2552
TspDTI ATGAA 8 cut(s) 6, 253, 651, 760, 1542, 1637, 1677, 1919
TspRI CASTG 7 cut(s) 600, 728, 877, 991, 1696, 2204, 2377
Tth111I GACNNNGTC 1 cut(s) 830
Van91I CCANNNNNTGG 1 cut(s) 196
VpaK11BI GGWCC 3 cut(s) 1032, 1732, 2472
XagI CCTNNNNNAGG 1 cut(s) 1643
XapI RAATTY 3 cut(s) 1001, 1131, 1297
XbaI TCTAGA 1 cut(s) 1501
XceI RCATGY 2 cut(s) 633, 1791
XmaJI CCTAGG 2 cut(s) 842, 863
XmiI GTMKAC 2 cut(s) 132, 1116
ZrmI AGTACT 2 cut(s) 904, 1760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.