Rw7G020930

DNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013533
Physical Location & Seq
Reverse (-)
24087543 .. 24091343
3801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G020930.1

Sequence Viewer

Length: 1155 bp
ATGGATTCCCGGGACGCCCCGCCGCAGCAGCCTCCGCCGCAGCAACCCAACATGATGGTGGGCCCCAATTCCTACCCTTCCCCCATGGCCAACCCCAACCCCAACAACAACGCCACCGCCACCGCCAACAGCAACAACAACAACAACTCCTCGGCCGCGATGTTGGGCGGCTGCGGGCCGAATTCGGGTCGGTTCCAGTTCAACACGGTGGCCCAGCAGCAGCCCGCATCGAAGCCGCAGCCGCTCGATTCCATGAGCCCGAGCCCGTTCGACGGATCCGGAGGGTTTAGCATCGACTCTTCTACGGCCTCGGCGGGGAAGAAGAAGCGAGGCAGGCCCAGAAAGTACTCCCCTGATGGCAACATTGCATTGGGATTGGCACCAACTCAGATTCCTGCCTCGGCTCCCGTTGCGGCGGCCGCCGCCGGCCCCCACGGAGAATCCAGCGGCACCATGTCGTCGGACCCCCCTGCCAAGAAGAACAGGGGGCGCCCGCCGGGCTCCGGAAAGAAGCAGCTCGACGCTTTGGGAGCTGGTGGAGTTGGGTTCACGCCTCATGTCATTATGGTGCAAGCCGGCGAGGATATAGCATCAAAGGTTATGTCCTTTTCACAGCAAGGACCACGCACAGTTTGTATCCTTTCTGCAAATGGTCCAATCGGCAATGTTACCCTTCGCCAGCCATCAATGTCTGGTGGTACAGTCACATATGAGGGTCGATTTGAGATTATTTCTCTGTCAGGCTCCTACTTGTTCTCTGAAAATAATGGTAACCGCAGCAGAACTGGTGGTCTGAGTGTGTCCCTGGCAGGGTCTGATGGTTCAGTGCTGGGCGGTGGAGTTGCGGGCATGCTATTGGCAGCAGGCCCGGTACAGGTGATTGTGGGTAGCTTTATTGCGGAAGGGAAAAAATCTAGCTCCAATTTGCTAAAATCAGGGCCTTCTTCTCCACCACCATCTCAAATGTTGAACTTTGGTGCACCTATGACAGCAGCTAGCCCTTCCTCTCAAGGAGGAGGCTCAACTGAGTCGTCTGATGAGAATGGTAGTAGCCCTCTTAACAAGGCGCCACCTGTGCTTTACAGTAACCCTAGTCAACCCATGCATAATATCACGATGTACCAACAATTATGGGCTGGGCAAACTCAACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

384

Amino Acids

38.92

Weight (kDa)

9.46

Isoelectric Point (pI)

61.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 184 - 299 3.3e-24 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013533)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G17950 AT5G46640
fragaria_vesca FvH4_5g22000
malus_domestica MD06G1033700.v1.1 MD16G1274800.v1.1
prunus_persica Prupe.5G037000_v2.0.a1
pyrus_communis pycom06g02780 pycom16g24490
rosa_chinensis RchiOBHm_Chr7g0209501
rosa_laevigata RLG00000003123
rosa_multiflora Rmu_sc0000243.1_g000005
rosa_roxburghii Rroxscaffold_3G00249310
rosa_rugosa Rorug07G0114000
rosa_samantha Rh7AG248000 Rh7BG242500 Rh7CG263500 Rh7DG254100
rosa_wichuraiana Rw7G020930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 379, 449, 489, 1066
AccBSI CCGCTC 1 cut(s) 244
AccII CGCG 1 cut(s) 158
AccIII TCCGGA 2 cut(s) 278, 503
AclWI GGATC 2 cut(s) 270, 283
AcoI YGGCCR 3 cut(s) 87, 153, 417
AcsI RAATTY 1 cut(s) 181
AcyI GRCGYC 3 cut(s) 15, 490, 1067
AfaI GTAC 4 cut(s) 347, 700, 873, 1121
AfiI CCNNNNNNNGG 6 cut(s) 185, 272, 315, 503, 810, 874
AgsI TTSAA 2 cut(s) 202, 970
AjnI CCWGG 1 cut(s) 804
AluBI AGCT 5 cut(s) 517, 533, 891, 918, 995
AluI AGCT 5 cut(s) 517, 533, 891, 918, 995
Alw21I GWGCWC 1 cut(s) 982
Alw44I GTGCAC 1 cut(s) 978
AlwI GGATC 2 cut(s) 270, 283
AlwNI CAGNNNCTG 1 cut(s) 815
Ama87I CYCGRG 2 cut(s) 9, 259
Aor13HI TCCGGA 2 cut(s) 278, 503
ApaI GGGCCC 1 cut(s) 65
ApaLI GTGCAC 1 cut(s) 978
ApoI RAATTY 1 cut(s) 181
ArsI GACNNNNNNTTYG 2 cut(s) 587, 619
AspLEI GCGC 2 cut(s) 492, 1069
AsuC2I CCSGG 4 cut(s) 10, 11, 498, 869
AsuHPI GGTGA 1 cut(s) 889
AsuNHI GCTAGC 1 cut(s) 995
AvaI CYCGRG 2 cut(s) 9, 259
AvaII GGWCC 3 cut(s) 463, 620, 653
BaeGI GKGCMC 2 cut(s) 65, 982
BalI TGGCCA 1 cut(s) 89
BamHI GGATCC 1 cut(s) 275
BanI GGYRCC 4 cut(s) 379, 449, 489, 1066
BanII GRGCYC 4 cut(s) 65, 260, 266, 503
Bbv12I GWGCWC 1 cut(s) 982
BccI CCATC 5 cut(s) 49, 350, 691, 812, 964
BceAI ACGGC 1 cut(s) 321
BciT130I CCWGG 1 cut(s) 806
BciVI GTATCC 1 cut(s) 647
BcnI CCSGG 4 cut(s) 10, 11, 498, 869
BfaI CTAG 3 cut(s) 915, 996, 1092
BfoI RGCGCY 2 cut(s) 493, 1070
BfuI GTATCC 1 cut(s) 647
BglI GCCNNNNNGGC 1 cut(s) 498
BmcAI AGTACT 1 cut(s) 347
Bme1390I CCNGG 5 cut(s) 10, 11, 498, 806, 869
Bme18I GGWCC 3 cut(s) 463, 620, 653
BmeT110I CYCGRG 2 cut(s) 9, 259
BmrFI CCNGG 5 cut(s) 10, 11, 498, 806, 869
BmsI GCATC 3 cut(s) 236, 300, 599
BmtI GCTAGC 1 cut(s) 999
BpuEI CTTGAG 1 cut(s) 993
BpuMI CCSGG 4 cut(s) 10, 11, 498, 869
BsaHI GRCGYC 3 cut(s) 15, 490, 1067
BsaJI CCNNGG 7 cut(s) 9, 84, 150, 309, 399, 433, 804
BsaWI WCCGGW 2 cut(s) 278, 503
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 6 cut(s) 185, 272, 315, 503, 810, 874
Bse118I RCCGGY 2 cut(s) 425, 575
Bse1I ACTGG 2 cut(s) 196, 790
Bse3DI GCAATG 2 cut(s) 363, 670
BseAI TCCGGA 2 cut(s) 278, 503
BseBI CCWGG 1 cut(s) 806
BseDI CCNNGG 7 cut(s) 9, 84, 150, 309, 399, 433, 804
BseLI CCNNNNNNNGG 6 cut(s) 185, 272, 315, 503, 810, 874
BseMI GCAATG 2 cut(s) 363, 670
BseMII CTCAG 3 cut(s) 401, 785, 1017
BseNI ACTGG 2 cut(s) 196, 790
BseRI GAGGAG 2 cut(s) 139, 1029
BseSI GKGCMC 2 cut(s) 65, 982
BseX3I CGGCCG 2 cut(s) 153, 417
BseYI CCCAGC 3 cut(s) 213, 829, 1136
Bsh1236I CGCG 1 cut(s) 158
Bsh1285I CGRYCG 2 cut(s) 156, 420
BshNI GGYRCC 4 cut(s) 379, 449, 489, 1066
BsiEI CGRYCG 2 cut(s) 156, 420
BsiHKAI GWGCWC 1 cut(s) 982
BsiHKCI CYCGRG 2 cut(s) 9, 259
BsiSI CCGG 7 cut(s) 10, 279, 426, 497, 504, 576, 869
BslFI GGGAC 2 cut(s) 26, 787
BslI CCNNNNNNNGG 6 cut(s) 185, 272, 315, 503, 810, 874
BsmFI GGGAC 2 cut(s) 26, 787
BsoBI CYCGRG 2 cut(s) 9, 259
Bsp120I GGGCCC 1 cut(s) 61
Bsp1286I GDGCHC 5 cut(s) 65, 260, 266, 503, 982
Bsp13I TCCGGA 2 cut(s) 278, 503
Bsp143I GATC 1 cut(s) 275
Bsp19I CCATGG 1 cut(s) 84
BspCNI CTCAG 3 cut(s) 400, 786, 1018
BspEI TCCGGA 2 cut(s) 278, 503
BspFNI CGCG 1 cut(s) 158
BspOI GCTAGC 1 cut(s) 999
BspPI GGATC 2 cut(s) 270, 283
BspT107I GGYRCC 4 cut(s) 379, 449, 489, 1066
BsrBI CCGCTC 1 cut(s) 244
BsrDI GCAATG 2 cut(s) 363, 670
BsrFI RCCGGY 2 cut(s) 425, 575
BsrI ACTGG 2 cut(s) 196, 790
BssAI RCCGGY 2 cut(s) 425, 575
BssECI CCNNGG 7 cut(s) 9, 84, 150, 309, 399, 433, 804
BssMI GATC 1 cut(s) 275
BssNI GRCGYC 3 cut(s) 15, 490, 1067
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 806
Bst4CI ACNGT 5 cut(s) 208, 631, 703, 1085, 1152
Bst6I CTCTTC 1 cut(s) 304
BstACI GRCGYC 3 cut(s) 15, 490, 1067
BstDEI CTNAG 3 cut(s) 387, 794, 1026
BstDSI CCRYGG 2 cut(s) 84, 433
BstEII GGTNACC 1 cut(s) 770
BstFNI CGCG 1 cut(s) 158
BstH2I RGCGCY 2 cut(s) 493, 1070
BstHHI GCGC 2 cut(s) 492, 1069
BstKTI GATC 1 cut(s) 278
BstMBI GATC 1 cut(s) 275
BstMCI CGRYCG 2 cut(s) 156, 420
BstNI CCWGG 1 cut(s) 806
BstNSI RCATGY 1 cut(s) 853
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 5 cut(s) 8, 9, 496, 804, 867
BstSLI GKGCMC 2 cut(s) 65, 982
BstUI CGCG 1 cut(s) 158
BstX2I RGATCY 1 cut(s) 275
BstXI CCANNNNNNTGG 1 cut(s) 55
BstYI RGATCY 1 cut(s) 275
BstZI CGGCCG 2 cut(s) 153, 417
BsuI GTATCC 1 cut(s) 647
BtgI CCRYGG 2 cut(s) 84, 433
BtgZI GCGATG 1 cut(s) 173
BtsIMutI CAGTG 1 cut(s) 831
CaiI CAGNNNCTG 1 cut(s) 815
CciNI GCGGCCGC 1 cut(s) 417
CfoI GCGC 2 cut(s) 492, 1069
Cfr10I RCCGGY 2 cut(s) 425, 575
Cfr9I CCCGGG 1 cut(s) 9
CseI GACGC 2 cut(s) 23, 530
Csp6I GTAC 4 cut(s) 346, 699, 872, 1120
CviAII CATG 7 cut(s) 52, 85, 253, 454, 557, 850, 1102
CviQI GTAC 4 cut(s) 346, 699, 872, 1120
DdeI CTNAG 3 cut(s) 387, 794, 1026
DinI GGCGCC 2 cut(s) 491, 1068
DpnI GATC 1 cut(s) 277
DpnII GATC 1 cut(s) 275
EaeI YGGCCR 3 cut(s) 87, 153, 417
EagI CGGCCG 2 cut(s) 153, 417
Eam1104I CTCTTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 304
EciI GGCGGA 1 cut(s) 24
EclXI CGGCCG 2 cut(s) 153, 417
Eco130I CCWWGG 1 cut(s) 84
Eco24I GRGCYC 4 cut(s) 65, 260, 266, 503
Eco47I GGWCC 3 cut(s) 463, 620, 653
Eco52I CGGCCG 2 cut(s) 153, 417
Eco88I CYCGRG 2 cut(s) 9, 259
Eco91I GGTNACC 1 cut(s) 770
EcoO109I RGGNCCY 2 cut(s) 62, 938
EcoO65I GGTNACC 1 cut(s) 770
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 804
EcoT14I CCWWGG 1 cut(s) 84
EcoT22I ATGCAT 1 cut(s) 1107
EcoT38I GRGCYC 4 cut(s) 65, 260, 266, 503
EgeI GGCGCC 2 cut(s) 491, 1068
EheI GGCGCC 2 cut(s) 491, 1068
ErhI CCWWGG 1 cut(s) 84
FaeI CATG 7 cut(s) 55, 88, 256, 457, 560, 853, 1105
FaqI GGGAC 2 cut(s) 26, 787
FatI CATG 7 cut(s) 51, 84, 252, 453, 556, 849, 1101
FauI CCCGC 6 cut(s) 27, 167, 232, 307, 501, 838
FauNDI CATATG 1 cut(s) 709
FriOI GRGCYC 4 cut(s) 65, 260, 266, 503
FspBI CTAG 3 cut(s) 915, 996, 1092
GlaI GCGC 2 cut(s) 491, 1068
GsaI CCCAGC 3 cut(s) 217, 833, 1140
HaeII RGCGCY 2 cut(s) 493, 1070
HapII CCGG 7 cut(s) 10, 279, 426, 497, 504, 576, 869
HgaI GACGC 2 cut(s) 23, 530
HhaI GCGC 2 cut(s) 492, 1069
Hin1I GRCGYC 3 cut(s) 15, 490, 1067
Hin1II CATG 7 cut(s) 55, 88, 256, 457, 560, 853, 1105
Hin6I GCGC 2 cut(s) 490, 1067
HinP1I GCGC 2 cut(s) 490, 1067
HincII GTYRAC 1 cut(s) 1097
HindII GTYRAC 1 cut(s) 1097
HinfI GANTC 6 cut(s) 5, 248, 296, 391, 440, 1028
HpaII CCGG 7 cut(s) 10, 279, 426, 497, 504, 576, 869
HphI GGTGA 1 cut(s) 889
Hpy166II GTNNAC 3 cut(s) 549, 980, 1097
Hpy188I TCNGA 6 cut(s) 390, 463, 760, 795, 817, 1036
Hpy188III TCNNGA 3 cut(s) 279, 504, 1114
Hpy8I GTNNAC 3 cut(s) 549, 980, 1097
Hpy99I CGWCG 3 cut(s) 275, 463, 524
HpyAV CCTTC 5 cut(s) 87, 683, 896, 951, 1011
HpyCH4III ACNGT 5 cut(s) 208, 631, 703, 1085, 1152
HpyCH4V TGCA 5 cut(s) 368, 571, 647, 980, 1105
HpyF3I CTNAG 3 cut(s) 387, 794, 1026
Hsp92I GRCGYC 3 cut(s) 15, 490, 1067
Hsp92II CATG 7 cut(s) 55, 88, 256, 457, 560, 853, 1105
HspAI GCGC 2 cut(s) 490, 1067
KasI GGCGCC 2 cut(s) 489, 1066
Kpn2I TCCGGA 2 cut(s) 278, 503
KroI GCCGGC 2 cut(s) 425, 575
KroNI GCCGGC 2 cut(s) 427, 577
Kzo9I GATC 1 cut(s) 275
LmnI GCTCC 5 cut(s) 409, 506, 530, 749, 923
LweI GCATC 3 cut(s) 236, 300, 599
MaeI CTAG 3 cut(s) 915, 996, 1092
MaeIII GTNAC 4 cut(s) 667, 703, 770, 1085
MalI GATC 1 cut(s) 277
MbiI CCGCTC 1 cut(s) 244
MboI GATC 1 cut(s) 275
MboII GAAGA 5 cut(s) 291, 331, 334, 490, 936
MflI RGATCY 1 cut(s) 275
MhlI GDGCHC 5 cut(s) 65, 260, 266, 503, 982
MlsI TGGCCA 1 cut(s) 89
MluCI AATT 4 cut(s) 67, 181, 922, 1127
MluNI TGGCCA 1 cut(s) 89
Mly113I GGCGCC 2 cut(s) 490, 1067
MlyI GAGTC 2 cut(s) 290, 1037
MmeI TCCRAC 1 cut(s) 441
Mox20I TGGCCA 1 cut(s) 89
Mph1103I ATGCAT 1 cut(s) 1107
MroI TCCGGA 2 cut(s) 278, 503
MroNI GCCGGC 2 cut(s) 425, 575
MscI TGGCCA 1 cut(s) 89
MseI TTAA 1 cut(s) 1059
MslI CAYNNNNRTG 2 cut(s) 56, 566
Msp20I TGGCCA 1 cut(s) 89
MspA1I CMGCKG 1 cut(s) 447
MspI CCGG 7 cut(s) 10, 279, 426, 497, 504, 576, 869
MspR9I CCNGG 5 cut(s) 10, 11, 498, 806, 869
MvaI CCWGG 1 cut(s) 806
MvnI CGCG 1 cut(s) 158
NaeI GCCGGC 2 cut(s) 427, 577
NarI GGCGCC 2 cut(s) 490, 1067
NciI CCSGG 4 cut(s) 10, 11, 498, 869
NcoI CCATGG 1 cut(s) 84
NdeI CATATG 1 cut(s) 709
NdeII GATC 1 cut(s) 275
NgoMIV GCCGGC 2 cut(s) 425, 575
NheI GCTAGC 1 cut(s) 995
NlaIII CATG 7 cut(s) 55, 88, 256, 457, 560, 853, 1105
NmeAIII GCCGAG 3 cut(s) 131, 290, 380
NmuCI GTSAC 1 cut(s) 703
NotI GCGGCCGC 1 cut(s) 417
NsiI ATGCAT 1 cut(s) 1107
NspI RCATGY 1 cut(s) 853
PaeI GCATGC 1 cut(s) 853
PdiI GCCGGC 2 cut(s) 427, 577
PfeI GAWTC 4 cut(s) 5, 248, 391, 440
PleI GAGTC 2 cut(s) 290, 1036
PluTI GGCGCC 2 cut(s) 493, 1070
PpsI GAGTC 2 cut(s) 290, 1036
Psp6I CCWGG 1 cut(s) 804
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 3 cut(s) 213, 829, 1136
PspGI CCWGG 1 cut(s) 804
PspOMI GGGCCC 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 815
PsuI RGATCY 1 cut(s) 275
RsaI GTAC 4 cut(s) 347, 700, 873, 1121
RsaNI GTAC 4 cut(s) 346, 699, 872, 1120
RseI CAYNNNNRTG 2 cut(s) 56, 566
SaqAI TTAA 1 cut(s) 1059
Sau3AI GATC 1 cut(s) 275
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 2 cut(s) 290, 1037
ScrFI CCNGG 5 cut(s) 10, 11, 498, 806, 869
SduI GDGCHC 5 cut(s) 65, 260, 266, 503, 982
SetI ASST 9 cut(s) 519, 535, 600, 879, 893, 920, 985, 997, 1075
SfaNI GCATC 3 cut(s) 236, 300, 599
SfoI GGCGCC 2 cut(s) 491, 1068
SinI GGWCC 3 cut(s) 463, 620, 653
SmaI CCCGGG 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 56, 566
SmlI CTYRAG 1 cut(s) 1008
SmoI CTYRAG 1 cut(s) 1008
SphI GCATGC 1 cut(s) 853
Sse9I AATT 4 cut(s) 67, 181, 922, 1127
SspDI GGCGCC 2 cut(s) 489, 1066
SspMI CTAG 3 cut(s) 915, 996, 1092
StyD4I CCNGG 5 cut(s) 8, 9, 496, 804, 867
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 5 cut(s) 208, 631, 703, 1085, 1152
TaqI TCGA 6 cut(s) 230, 246, 270, 294, 519, 718
TasI AATT 4 cut(s) 67, 181, 922, 1127
TatI WGTACW 1 cut(s) 345
TfiI GAWTC 4 cut(s) 5, 248, 391, 440
Tru1I TTAA 1 cut(s) 1059
Tru9I TTAA 1 cut(s) 1059
TscAI CASTG 1 cut(s) 831
TseFI GTSAC 1 cut(s) 703
Tsp45I GTSAC 1 cut(s) 703
TspGWI ACGGA 2 cut(s) 288, 450
TspMI CCCGGG 1 cut(s) 9
TspRI CASTG 1 cut(s) 831
VneI GTGCAC 1 cut(s) 978
VpaK11BI GGWCC 3 cut(s) 463, 620, 653
XapI RAATTY 1 cut(s) 181
XceI RCATGY 1 cut(s) 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XmaI CCCGGG 1 cut(s) 9
XspI CTAG 3 cut(s) 915, 996, 1092
ZrmI AGTACT 1 cut(s) 347
Zsp2I ATGCAT 1 cut(s) 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.