AT5G46720

Gamma-glutamyl cyclotransferase, AIG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007085
Physical Location & Seq
Forward (+)
18959094 .. 18959852
759 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G46720.1

Sequence Viewer

Length: 528 bp
ATGGGAGGATCAAACAAGAACCTCATATTCGTGTACGGAACGTTAAAAAGAAACCATCGAAACCATTTCCTCCTAGAAGATCTGATCTCAACAAACGACGCCGTTTACATAGGACAACGCACGACCCGGTTACAATACCCGCTCGTTACAGGTCTCTATGGGATCCCTTACTTAATCAACAAATCCGGGTCGGGTCAAAAGATCCGAGGCGAGCTTTATTCGGTTTCAAAACGTGGGCTTGTACGTCTTGATGAATTGGAAGGTATCAAAGTTAATCACTACGAGAGATTACCAATAGAAGTTATTGAGGAAGATGATGAAGAAGAAGAATCAAACGGTGTCGTCTTGGCTGAGGCTTATTTTGCTCACTTTGGATTTGGTGAGAGATTGTGGGAGAAGAAAGGTAAATGTGGGATGTGTGAATTTGGTGAAAACGATGGCGTTTTGTATGTTCGTCCTAAAGATAGGCCAATGTTTAGCTCTGTTCTTGATGAAATTGAAACTTTTGTGTCTTCTAGTAATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

175

Amino Acids

19.92

Weight (kDa)

5.21

Isoelectric Point (pI)

36.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 9 - 122 4e-17 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 142
AciI CCGC 1 cut(s) 140
AclI AACGTT 1 cut(s) 41
AclWI GGATC 4 cut(s) 16, 157, 170, 196
AcsI RAATTY 1 cut(s) 422
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 2 cut(s) 35, 243
AgsI TTSAA 2 cut(s) 228, 500
AluBI AGCT 2 cut(s) 214, 480
AluI AGCT 2 cut(s) 214, 480
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 4 cut(s) 16, 157, 170, 196
AoxI GGCC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 422
AsuC2I CCSGG 2 cut(s) 127, 187
AsuHPI GGTGA 2 cut(s) 392, 440
BamHI GGATCC 1 cut(s) 162
BbsI GAAGAC 1 cut(s) 504
BbvCI CCTCAGC 1 cut(s) 351
BccI CCATC 2 cut(s) 63, 431
BceAI ACGGC 1 cut(s) 86
BcnI CCSGG 2 cut(s) 127, 187
BcoDI GTCTC 1 cut(s) 158
BfaI CTAG 2 cut(s) 74, 516
BglII AGATCT 1 cut(s) 79
Bme1390I CCNGG 2 cut(s) 127, 187
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 2 cut(s) 127, 187
BpiI GAAGAC 1 cut(s) 504
Bpu10I CCTNAGC 1 cut(s) 351
BpuMI CCSGG 2 cut(s) 127, 187
BsaHI GRCGYC 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 158
BsaJI CCNNGG 1 cut(s) 205
BseDI CCNNGG 1 cut(s) 205
BseGI GGATG 1 cut(s) 420
BseMII CTCAG 1 cut(s) 342
BshFI GGCC 1 cut(s) 469
BsiSI CCGG 2 cut(s) 127, 186
BsmAI GTCTC 1 cut(s) 158
BsnI GGCC 1 cut(s) 469
Bso31I GGTCTC 1 cut(s) 158
Bsp143I GATC 5 cut(s) 8, 79, 84, 162, 201
BspACI CCGC 1 cut(s) 140
BspANI GGCC 1 cut(s) 469
BspCNI CTCAG 1 cut(s) 343
BspLI GGNNCC 1 cut(s) 164
BspPI GGATC 4 cut(s) 16, 157, 170, 196
BspTNI GGTCTC 1 cut(s) 158
BsrBI CCGCTC 1 cut(s) 142
BssECI CCNNGG 1 cut(s) 205
BssMI GATC 5 cut(s) 8, 79, 84, 162, 201
BssNI GRCGYC 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 338
BstACI GRCGYC 1 cut(s) 99
BstC8I GCNNGC 1 cut(s) 212
BstDEI CTNAG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 420
BstKTI GATC 5 cut(s) 11, 82, 87, 165, 204
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 5 cut(s) 8, 79, 84, 162, 201
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstSCI CCNGG 2 cut(s) 125, 185
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 3 cut(s) 79, 162, 201
BstYI RGATCY 3 cut(s) 79, 162, 201
BsuRI GGCC 1 cut(s) 469
BtsCI GGATG 1 cut(s) 420
Cac8I GCNNGC 1 cut(s) 212
CseI GACGC 1 cut(s) 107
Csp6I GTAC 2 cut(s) 34, 242
CviJI RGCY 6 cut(s) 214, 238, 350, 356, 469, 480
CviKI_1 RGCY 6 cut(s) 214, 238, 350, 356, 469, 480
CviQI GTAC 2 cut(s) 34, 242
DdeI CTNAG 1 cut(s) 351
DpnI GATC 5 cut(s) 10, 81, 86, 164, 203
DpnII GATC 5 cut(s) 8, 79, 84, 162, 201
Eco31I GGTCTC 1 cut(s) 158
FaiI YATR 4 cut(s) 26, 110, 159, 450
FauI CCCGC 1 cut(s) 147
FokI GGATG 1 cut(s) 427
FspBI CTAG 2 cut(s) 74, 516
HaeIII GGCC 1 cut(s) 469
HapII CCGG 2 cut(s) 127, 186
HgaI GACGC 1 cut(s) 107
Hin1I GRCGYC 1 cut(s) 99
HinfI GANTC 1 cut(s) 329
HpaII CCGG 2 cut(s) 127, 186
HphI GGTGA 2 cut(s) 392, 440
Hpy166II GTNNAC 2 cut(s) 34, 106
Hpy188I TCNGA 2 cut(s) 84, 206
Hpy188III TCNNGA 2 cut(s) 248, 488
Hpy8I GTNNAC 2 cut(s) 34, 106
Hpy99I CGWCG 1 cut(s) 101
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4IV ACGT 3 cut(s) 41, 232, 244
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 1 cut(s) 351
HpySE526I ACGT 3 cut(s) 41, 232, 244
Hsp92I GRCGYC 1 cut(s) 99
Kzo9I GATC 5 cut(s) 8, 79, 84, 162, 201
LpnPI CCDG 3 cut(s) 135, 140, 199
MaeI CTAG 2 cut(s) 74, 516
MaeII ACGT 3 cut(s) 41, 232, 244
MaeIII GTNAC 2 cut(s) 129, 145
MalI GATC 5 cut(s) 10, 81, 86, 164, 203
MbiI CCGCTC 1 cut(s) 142
MboI GATC 5 cut(s) 8, 79, 84, 162, 201
MboII GAAGA 7 cut(s) 89, 323, 332, 335, 338, 409, 504
MflI RGATCY 3 cut(s) 79, 162, 201
MluCI AATT 3 cut(s) 254, 422, 495
MnlI CCTC 5 cut(s) 32, 80, 200, 301, 346
MseI TTAA 3 cut(s) 44, 173, 273
MslI CAYNNNNRTG 1 cut(s) 29
MspI CCGG 2 cut(s) 127, 186
MspR9I CCNGG 2 cut(s) 127, 187
MwoI GCNNNNNNNGC 1 cut(s) 362
NciI CCSGG 2 cut(s) 127, 187
NdeII GATC 5 cut(s) 8, 79, 84, 162, 201
NlaIV GGNNCC 1 cut(s) 164
PfeI GAWTC 1 cut(s) 329
Psp1406I AACGTT 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 164
PsuI RGATCY 3 cut(s) 79, 162, 201
RsaI GTAC 2 cut(s) 35, 243
RsaNI GTAC 2 cut(s) 34, 242
RseI CAYNNNNRTG 1 cut(s) 29
SaqAI TTAA 3 cut(s) 44, 173, 273
Sau3AI GATC 5 cut(s) 8, 79, 84, 162, 201
ScrFI CCNGG 2 cut(s) 127, 187
SetI ASST 9 cut(s) 24, 44, 154, 216, 235, 247, 265, 406, 482
SmiMI CAYNNNNRTG 1 cut(s) 29
Sse9I AATT 3 cut(s) 254, 422, 495
SsiI CCGC 1 cut(s) 140
SspMI CTAG 2 cut(s) 74, 516
StyD4I CCNGG 2 cut(s) 125, 185
TaaI ACNGT 1 cut(s) 338
TaiI ACGT 3 cut(s) 44, 235, 247
TaqI TCGA 1 cut(s) 58
TasI AATT 3 cut(s) 254, 422, 495
TfiI GAWTC 1 cut(s) 329
Tru1I TTAA 3 cut(s) 44, 173, 273
Tru9I TTAA 3 cut(s) 44, 173, 273
TspDTI ATGAA 3 cut(s) 267, 333, 507
TspGWI ACGGA 1 cut(s) 51
XapI RAATTY 1 cut(s) 422
XspI CTAG 2 cut(s) 74, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.