MD16G1273600.v1.1

Gamma-glutamyl cyclotransferase, AIG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007085
Physical Location & Seq
Forward (+)
36406792 .. 36407325
534 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1273600.v1.1.491

Sequence Viewer

Length: 534 bp
ATGGCGATGGCGGCTGAGAGTCTGACAGACGAATCCAAACCCCAGCTAATCTTCAGTTACGGCACACTCAAACAAGGCTTCCACAACCACAAACTAACCCAGGACCTCATCGACCGAAACGACGCCGTGTCACTAGGCCCCTGCATCACCCAATTCTCCTACCCTCTCGTTTGCGGACCCCACGGAATCCCCTACCTTATCAACCTTCCCGGGTCGGGCCACCGGATCAAGGGCGAGCTCTACTCTGTCTCAACCCGCGGACTCGCCCGGTTCGACGAGTTGGAGGGCACCAGCATCGGCCACTACGAGCGCCTCCCTATTCACGTAATCCATGGCAGCGGAGACGGCGTCGTTTCGGCTGAGGCTTATTTCGCGCACAGAAGCTTTGGCCCTGCGATGTGGGAGAGAAATGGCAAGAAGGGTTTGAGCGAGTGGACGGAGAATGAGGCCAGAGGGTATGTGAAAAGACAAGATAGGCCATACCAGGGTCGCACCATTCTCGAGGACGTCCGACAATTTGTTTCCAGGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

178

Amino Acids

19.86

Weight (kDa)

7.85

Isoelectric Point (pI)

31.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 17 - 127 1.6e-16 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 510
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 2 cut(s) 258, 374
AciI CCGC 5 cut(s) 11, 174, 256, 258, 339
AclWI GGATC 1 cut(s) 233
AcoI YGGCCR 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 37
AcyI GRCGYC 3 cut(s) 123, 348, 507
AfiI CCNNNNNNNGG 3 cut(s) 215, 229, 485
AhdI GACNNNNNGTC 1 cut(s) 127
AjnI CCWGG 3 cut(s) 99, 483, 524
AluBI AGCT 3 cut(s) 46, 238, 384
AluI AGCT 3 cut(s) 46, 238, 384
Alw21I GWGCWC 1 cut(s) 240
Alw26I GTCTC 2 cut(s) 253, 336
AlwI GGATC 1 cut(s) 233
Ama87I CYCGRG 2 cut(s) 209, 500
AoxI GGCC 6 cut(s) 136, 217, 298, 388, 447, 476
ApeKI GCWGC 1 cut(s) 336
AspLEI GCGC 2 cut(s) 312, 376
AspS9I GGNCC 5 cut(s) 103, 137, 176, 217, 389
AsuC2I CCSGG 3 cut(s) 210, 211, 268
AsuHPI GGTGA 1 cut(s) 139
AvaI CYCGRG 2 cut(s) 209, 500
AvaII GGWCC 2 cut(s) 103, 176
BaeGI GKGCMC 1 cut(s) 290
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 240
BbvCI CCTCAGC 1 cut(s) 360
BbvI GCAGC 1 cut(s) 348
BceAI ACGGC 3 cut(s) 76, 110, 361
BcgI CGANNNNNNTGC 4 cut(s) 277, 311, 481, 515
BciT130I CCWGG 3 cut(s) 101, 485, 526
BcnI CCSGG 3 cut(s) 210, 211, 268
BcoDI GTCTC 2 cut(s) 253, 336
BfaI CTAG 1 cut(s) 134
BfoI RGCGCY 1 cut(s) 313
BisI GCNGC 2 cut(s) 12, 337
BlsI GCNGC 2 cut(s) 13, 338
Bme1390I CCNGG 6 cut(s) 101, 210, 211, 268, 485, 526
Bme18I GGWCC 2 cut(s) 103, 176
BmeRI GACNNNNNGTC 1 cut(s) 127
BmeT110I CYCGRG 2 cut(s) 209, 500
BmgT120I GGNCC 5 cut(s) 103, 137, 176, 217, 389
BmiI GGNNCC 3 cut(s) 139, 178, 289
BmrFI CCNGG 6 cut(s) 101, 210, 211, 268, 485, 526
BmsI GCATC 2 cut(s) 153, 303
BplI GAGNNNNNCTC 2 cut(s) 227, 259
Bpu10I CCTNAGC 1 cut(s) 360
BpuMI CCSGG 3 cut(s) 210, 211, 268
BsaAI YACGTR 1 cut(s) 325
BsaHI GRCGYC 3 cut(s) 123, 348, 507
BsaJI CCNNGG 6 cut(s) 99, 181, 209, 256, 331, 484
BsaWI WCCGGW 1 cut(s) 222
BsaXI ACNNNNNCTCC 2 cut(s) 333, 363
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 229, 485
BseBI CCWGG 3 cut(s) 101, 485, 526
BseDI CCNNGG 6 cut(s) 99, 181, 209, 256, 331, 484
BseLI CCNNNNNNNGG 3 cut(s) 215, 229, 485
BseMII CTCAG 2 cut(s) 6, 351
BseSI GKGCMC 1 cut(s) 290
BseXI GCAGC 1 cut(s) 348
BseYI CCCAGC 1 cut(s) 42
Bsh1236I CGCG 2 cut(s) 258, 374
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 6 cut(s) 138, 219, 300, 390, 449, 478
BshNI GGYRCC 1 cut(s) 287
BsiEI CGRYCG 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 240
BsiHKCI CYCGRG 2 cut(s) 209, 500
BsiSI CCGG 3 cut(s) 210, 223, 268
BslI CCNNNNNNNGG 3 cut(s) 215, 229, 485
BsmAI GTCTC 2 cut(s) 253, 336
BsmBI CGTCTC 1 cut(s) 336
BsnI GGCC 6 cut(s) 138, 219, 300, 390, 449, 478
BsoBI CYCGRG 2 cut(s) 209, 500
Bsp1286I GDGCHC 2 cut(s) 240, 290
Bsp143I GATC 1 cut(s) 225
Bsp19I CCATGG 1 cut(s) 331
BspACI CCGC 5 cut(s) 11, 174, 256, 258, 339
BspANI GGCC 6 cut(s) 138, 219, 300, 390, 449, 478
BspCNI CTCAG 2 cut(s) 7, 352
BspFNI CGCG 2 cut(s) 258, 374
BspLI GGNNCC 3 cut(s) 139, 178, 289
BspPI GGATC 1 cut(s) 233
BspT107I GGYRCC 1 cut(s) 287
BssECI CCNNGG 6 cut(s) 99, 181, 209, 256, 331, 484
BssMI GATC 1 cut(s) 225
BssNI GRCGYC 3 cut(s) 123, 348, 507
BssT1I CCWWGG 1 cut(s) 331
Bst2UI CCWGG 3 cut(s) 101, 485, 526
BstACI GRCGYC 3 cut(s) 123, 348, 507
BstBAI YACGTR 1 cut(s) 325
BstC8I GCNNGC 1 cut(s) 236
BstDEI CTNAG 2 cut(s) 15, 360
BstDSI CCRYGG 3 cut(s) 181, 256, 331
BstFNI CGCG 2 cut(s) 258, 374
BstH2I RGCGCY 1 cut(s) 313
BstHHI GCGC 2 cut(s) 312, 376
BstKTI GATC 1 cut(s) 228
BstMAI GTCTC 2 cut(s) 253, 336
BstMBI GATC 1 cut(s) 225
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 3 cut(s) 11, 345, 371
BstNI CCWGG 3 cut(s) 101, 485, 526
BstSCI CCNGG 6 cut(s) 99, 208, 209, 266, 483, 524
BstSLI GKGCMC 1 cut(s) 290
BstUI CGCG 2 cut(s) 258, 374
BstV1I GCAGC 1 cut(s) 348
BsuRI GGCC 6 cut(s) 138, 219, 300, 390, 449, 478
BtgI CCRYGG 3 cut(s) 181, 256, 331
BtgZI GCGATG 2 cut(s) 20, 410
Cac8I GCNNGC 1 cut(s) 236
CfoI GCGC 2 cut(s) 312, 376
Cfr13I GGNCC 5 cut(s) 103, 137, 176, 217, 389
Cfr42I CCGCGG 1 cut(s) 259
Cfr9I CCCGGG 1 cut(s) 209
CseI GACGC 2 cut(s) 131, 337
CviAII CATG 1 cut(s) 332
DdeI CTNAG 2 cut(s) 15, 360
DpnI GATC 1 cut(s) 227
DpnII GATC 1 cut(s) 225
DriI GACNNNNNGTC 1 cut(s) 127
EaeI YGGCCR 1 cut(s) 298
Eam1105I GACNNNNNGTC 1 cut(s) 127
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 331
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 2 cut(s) 103, 176
Eco53kI GAGCTC 1 cut(s) 238
Eco57I CTGAAG 1 cut(s) 37
Eco88I CYCGRG 2 cut(s) 209, 500
EcoICRI GAGCTC 1 cut(s) 238
EcoO109I RGGNCCY 2 cut(s) 103, 137
EcoRII CCWGG 3 cut(s) 99, 483, 524
EcoT14I CCWWGG 1 cut(s) 331
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 331
Esp3I CGTCTC 1 cut(s) 336
FaeI CATG 1 cut(s) 335
FaiI YATR 3 cut(s) 333, 459, 481
FatI CATG 1 cut(s) 331
FauI CCCGC 1 cut(s) 263
Fnu4HI GCNGC 2 cut(s) 12, 337
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 2 cut(s) 12, 337
FspBI CTAG 1 cut(s) 134
GlaI GCGC 2 cut(s) 311, 375
GluI GCNGC 2 cut(s) 12, 337
GsaI CCCAGC 1 cut(s) 46
HaeII RGCGCY 1 cut(s) 313
HaeIII GGCC 6 cut(s) 138, 219, 300, 390, 449, 478
HapII CCGG 3 cut(s) 210, 223, 268
HgaI GACGC 2 cut(s) 131, 337
HhaI GCGC 2 cut(s) 312, 376
Hin1I GRCGYC 3 cut(s) 123, 348, 507
Hin1II CATG 1 cut(s) 335
Hin6I GCGC 2 cut(s) 310, 374
HinP1I GCGC 2 cut(s) 310, 374
HindIII AAGCTT 1 cut(s) 382
HinfI GANTC 4 cut(s) 19, 32, 186, 261
HpaII CCGG 3 cut(s) 210, 223, 268
HphI GGTGA 1 cut(s) 139
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 2 cut(s) 24, 512
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 1 cut(s) 435
Hpy99I CGWCG 3 cut(s) 125, 278, 353
HpyAV CCTTC 2 cut(s) 215, 412
HpyCH4IV ACGT 2 cut(s) 324, 507
HpyCH4V TGCA 1 cut(s) 144
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 345, 371
HpyF3I CTNAG 2 cut(s) 15, 360
HpySE526I ACGT 2 cut(s) 324, 507
Hsp92I GRCGYC 3 cut(s) 123, 348, 507
Hsp92II CATG 1 cut(s) 335
HspAI GCGC 2 cut(s) 310, 374
KspI CCGCGG 1 cut(s) 259
Kzo9I GATC 1 cut(s) 225
Lsp1109I GCAGC 1 cut(s) 348
LweI GCATC 2 cut(s) 153, 303
MaeI CTAG 1 cut(s) 134
MaeII ACGT 2 cut(s) 324, 507
MaeIII GTNAC 2 cut(s) 56, 129
MalI GATC 1 cut(s) 227
MboI GATC 1 cut(s) 225
MboII GAAGA 1 cut(s) 43
MhlI GDGCHC 2 cut(s) 240, 290
MluCI AATT 2 cut(s) 152, 515
MlyI GAGTC 2 cut(s) 28, 255
MmeI TCCRAC 1 cut(s) 261
MnlI CCTC 8 cut(s) 116, 174, 277, 323, 355, 439, 446, 496
MspA1I CMGCKG 2 cut(s) 258, 339
MspI CCGG 3 cut(s) 210, 223, 268
MspR9I CCNGG 6 cut(s) 101, 210, 211, 268, 485, 526
MvaI CCWGG 3 cut(s) 101, 485, 526
MvnI CGCG 2 cut(s) 258, 374
MwoI GCNNNNNNNGC 3 cut(s) 11, 345, 371
NciI CCSGG 3 cut(s) 210, 211, 268
NcoI CCATGG 1 cut(s) 331
NdeII GATC 1 cut(s) 225
NlaIII CATG 1 cut(s) 335
NlaIV GGNNCC 3 cut(s) 139, 178, 289
NmuCI GTSAC 1 cut(s) 129
PaeR7I CTCGAG 1 cut(s) 500
PcsI WCGNNNNNNNCGW 3 cut(s) 117, 270, 303
PfeI GAWTC 2 cut(s) 32, 186
PflFI GACNNNGTC 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 524
PkrI GCNGC 2 cut(s) 13, 338
PleI GAGTC 2 cut(s) 27, 255
PpsI GAGTC 2 cut(s) 27, 255
Ppu21I YACGTR 1 cut(s) 325
PpuMI RGGWCCY 1 cut(s) 103
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 103
Psp6I CCWGG 3 cut(s) 99, 483, 524
PspFI CCCAGC 1 cut(s) 42
PspGI CCWGG 3 cut(s) 99, 483, 524
PspN4I GGNNCC 3 cut(s) 139, 178, 289
PspPI GGNCC 5 cut(s) 103, 137, 176, 217, 389
PspPPI RGGWCCY 1 cut(s) 103
PsyI GACNNNGTC 1 cut(s) 347
SacI GAGCTC 1 cut(s) 240
SacII CCGCGG 1 cut(s) 259
SatI GCNGC 2 cut(s) 12, 337
Sau3AI GATC 1 cut(s) 225
Sau96I GGNCC 5 cut(s) 103, 137, 176, 217, 389
SchI GAGTC 2 cut(s) 28, 255
ScrFI CCNGG 6 cut(s) 101, 210, 211, 268, 485, 526
SduI GDGCHC 2 cut(s) 240, 290
SetI ASST 8 cut(s) 48, 108, 198, 207, 240, 327, 386, 510
SfaNI GCATC 2 cut(s) 153, 303
Sfr274I CTCGAG 1 cut(s) 500
Sfr303I CCGCGG 1 cut(s) 259
SgrBI CCGCGG 1 cut(s) 259
SinI GGWCC 2 cut(s) 103, 176
SlaI CTCGAG 1 cut(s) 500
SmaI CCCGGG 1 cut(s) 211
SmlI CTYRAG 1 cut(s) 500
SmoI CTYRAG 1 cut(s) 500
Sse9I AATT 2 cut(s) 152, 515
SsiI CCGC 5 cut(s) 11, 174, 256, 258, 339
SspMI CTAG 1 cut(s) 134
SstI GAGCTC 1 cut(s) 240
StyD4I CCNGG 6 cut(s) 99, 208, 209, 266, 483, 524
StyI CCWWGG 1 cut(s) 331
TaiI ACGT 2 cut(s) 327, 510
TaqI TCGA 3 cut(s) 111, 273, 501
TaqII GACCGA 1 cut(s) 129
TasI AATT 2 cut(s) 152, 515
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 2 cut(s) 32, 186
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 1 cut(s) 336
Tsp45I GTSAC 1 cut(s) 129
TspGWI ACGGA 2 cut(s) 198, 452
TspMI CCCGGG 1 cut(s) 209
Tth111I GACNNNGTC 1 cut(s) 347
VpaK11BI GGWCC 2 cut(s) 103, 176
XhoI CTCGAG 1 cut(s) 500
XmaI CCCGGG 1 cut(s) 209
XspI CTAG 1 cut(s) 134
ZraI GACGTC 1 cut(s) 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.