Rroxscaffold_3G00248890

Gamma-glutamyl cyclotransferase, AIG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007085
Physical Location & Seq
Forward (+)
42354867 .. 42356929
2063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00248890.1

Sequence Viewer

Length: 762 bp
ATGGCTGACATCTCCGACGACGAATCCAAACCCCACCTCATCTTCTCCTACGGCACACTCAAGCGAGGCTTCCACAACCACAAGCTGACCAAGGAATTAATCGACCAAGACGACGCCGTTTTCATCGGCCCCCACATTACCCTCCACACCTACCCCCTCGTCTGCGGCCCCCACGGATTGCCCTACCTCATCAACCTCCCCGGGTCGGGTCACCGGATCAGGGGCGAGCTCTACGCCGTTTCGACCCGGGGACTCGCCCGGTTCGACGAGCTGGAATGCACCTCCATCGGGCACTACGAGCGGCTACCGGTTCAATTGACGGTCAGTGCGGACAGAGACGACGTCGTTCCGGTCGAGGCCGAGGCCTATTTCGCGCACAGAAGCTTCGGGGAGGCCATGTGGGAGAGGAACGGGAAGGAGGGTTTGAGCGAGTTCACGGAGGAGGAGGCCAAAGGGTACGTGAAAAGACAGGATAGGCCTCATCAGGGTCGCAATATTCTCGAGGACGTCCGGCATTTTGTGAATGGAGATTGTGATACAAATGCAGTGATACCTAGCCCAGTAGCTGTCCTATTGTTAACCAGCTGGGTAGCCAAGAAGGAATTCTTTCGAATGGCTTGGCTGAACAAGATGGATGGATACCTAACCAACTCAGATATTGAAGGGGCCTGGTTCTTGTATAGTTCCATGTGTGTGGACACCTCTCTCGGCCAGAATTGCTGGGTATCCAAACAAAGAATCTCATCTCATCTGCAGTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

253

Amino Acids

28.66

Weight (kDa)

5.72

Isoelectric Point (pI)

44.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 14 - 127 3.6e-18 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 345, 510
AccBSI CCGCTC 1 cut(s) 301
AccII CGCG 1 cut(s) 374
AciI CCGC 3 cut(s) 165, 301, 329
AclWI GGATC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 709
AcsI RAATTY 1 cut(s) 602
AcyI GRCGYC 3 cut(s) 114, 342, 507
AfaI GTAC 1 cut(s) 458
AfiI CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
AgeI ACCGGT 1 cut(s) 307
AgsI TTSAA 2 cut(s) 314, 662
AjnI CCWGG 1 cut(s) 668
AjuI GAANNNNNNNTTGG 2 cut(s) 587, 619
AluBI AGCT 6 cut(s) 85, 229, 271, 384, 566, 585
AluI AGCT 6 cut(s) 85, 229, 271, 384, 566, 585
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 330
AlwI GGATC 1 cut(s) 224
AlwNI CAGNNNCTG 1 cut(s) 566
Ama87I CYCGRG 3 cut(s) 200, 246, 500
AoxI GGCC 9 cut(s) 127, 166, 357, 363, 393, 447, 476, 666, 709
ApoI RAATTY 1 cut(s) 602
AseI ATTAAT 1 cut(s) 98
AsiGI ACCGGT 1 cut(s) 307
Asp700I GAANNNNTTC 2 cut(s) 602, 606
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 3 cut(s) 128, 167, 666
AsuC2I CCSGG 5 cut(s) 201, 202, 247, 248, 259
AsuHPI GGTGA 1 cut(s) 203
AsuII TTCGAA 1 cut(s) 610
AvaI CYCGRG 3 cut(s) 200, 246, 500
BaeGI GKGCMC 1 cut(s) 294
BanII GRGCYC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 231
BccI CCATC 3 cut(s) 293, 625, 629
BceAI ACGGC 3 cut(s) 67, 101, 221
BcgI CGANNNNNNTGC 4 cut(s) 268, 302, 481, 515
BciT130I CCWGG 1 cut(s) 670
BciVI GTATCC 2 cut(s) 632, 736
BcnI CCSGG 5 cut(s) 201, 202, 247, 248, 259
BcoDI GTCTC 1 cut(s) 330
BfaI CTAG 2 cut(s) 555, 760
BfmI CTRYAG 1 cut(s) 752
BfuI GTATCC 2 cut(s) 632, 736
BisI GCNGC 2 cut(s) 166, 302
BlsI GCNGC 2 cut(s) 167, 303
Bme1390I CCNGG 6 cut(s) 201, 202, 247, 248, 259, 670
BmeT110I CYCGRG 3 cut(s) 200, 246, 500
BmgT120I GGNCC 3 cut(s) 128, 167, 666
BmiI GGNNCC 3 cut(s) 130, 169, 667
BmrFI CCNGG 6 cut(s) 201, 202, 247, 248, 259, 670
BmrI ACTGGG 1 cut(s) 554
BmuI ACTGGG 1 cut(s) 554
Bpu14I TTCGAA 1 cut(s) 610
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 5 cut(s) 201, 202, 247, 248, 259
BsaAI YACGTR 1 cut(s) 460
BsaHI GRCGYC 3 cut(s) 114, 342, 507
BsaJI CCNNGG 7 cut(s) 90, 172, 199, 200, 246, 247, 360
BsaWI WCCGGW 3 cut(s) 213, 307, 349
BsaXI ACNNNNNCTCC 2 cut(s) 383, 413
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
Bse118I RCCGGY 1 cut(s) 307
Bse1I ACTGG 1 cut(s) 560
BseBI CCWGG 1 cut(s) 670
BseDI CCNNGG 7 cut(s) 90, 172, 199, 200, 246, 247, 360
BseGI GGATG 1 cut(s) 640
BseLI CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 1 cut(s) 560
BseRI GAGGAG 2 cut(s) 455, 458
BseSI GKGCMC 1 cut(s) 294
BseYI CCCAGC 2 cut(s) 585, 720
Bsh1236I CGCG 1 cut(s) 374
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 9 cut(s) 129, 168, 359, 365, 395, 449, 478, 668, 711
BshTI ACCGGT 1 cut(s) 307
BsiEI CGRYCG 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 3 cut(s) 200, 246, 500
BsiSI CCGG 7 cut(s) 201, 214, 247, 259, 308, 350, 511
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
BsmAI GTCTC 1 cut(s) 330
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 1 cut(s) 264
BsmI GAATGC 1 cut(s) 281
BsnI GGCC 9 cut(s) 129, 168, 359, 365, 395, 449, 478, 668, 711
BsoBI CYCGRG 3 cut(s) 200, 246, 500
Bsp119I TTCGAA 1 cut(s) 610
Bsp1286I GDGCHC 2 cut(s) 231, 294
Bsp143I GATC 1 cut(s) 216
BspACI CCGC 3 cut(s) 165, 301, 329
BspANI GGCC 9 cut(s) 129, 168, 359, 365, 395, 449, 478, 668, 711
BspCNI CTCAG 1 cut(s) 665
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 3 cut(s) 130, 169, 667
BspMAI CTGCAG 1 cut(s) 756
BspPI GGATC 1 cut(s) 224
BspT104I TTCGAA 1 cut(s) 610
BsrBI CCGCTC 1 cut(s) 301
BsrFI RCCGGY 1 cut(s) 307
BsrI ACTGG 1 cut(s) 560
BssAI RCCGGY 1 cut(s) 307
BssECI CCNNGG 7 cut(s) 90, 172, 199, 200, 246, 247, 360
BssMI GATC 1 cut(s) 216
BssNI GRCGYC 3 cut(s) 114, 342, 507
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 670
Bst4CI ACNGT 1 cut(s) 322
BstACI GRCGYC 3 cut(s) 114, 342, 507
BstBAI YACGTR 1 cut(s) 460
BstBI TTCGAA 1 cut(s) 610
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 1 cut(s) 652
BstDSI CCRYGG 1 cut(s) 172
BstEII GGTNACC 1 cut(s) 209
BstF5I GGATG 1 cut(s) 640
BstFNI CGCG 1 cut(s) 374
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 1 cut(s) 216
BstMCI CGRYCG 1 cut(s) 354
BstMWI GCNNNNNNNGC 3 cut(s) 298, 371, 717
BstNI CCWGG 1 cut(s) 670
BstPI GGTNACC 1 cut(s) 209
BstSCI CCNGG 6 cut(s) 199, 200, 245, 246, 257, 668
BstSFI CTRYAG 1 cut(s) 752
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 1 cut(s) 374
BstXI CCANNNNNNTGG 1 cut(s) 694
BsuI GTATCC 2 cut(s) 632, 736
BsuRI GGCC 9 cut(s) 129, 168, 359, 365, 395, 449, 478, 668, 711
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 1 cut(s) 640
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 2 cut(s) 331, 552
Cac8I GCNNGC 1 cut(s) 227
CaiI CAGNNNCTG 1 cut(s) 566
CfoI GCGC 1 cut(s) 376
Cfr10I RCCGGY 1 cut(s) 307
Cfr13I GGNCC 3 cut(s) 128, 167, 666
Cfr9I CCCGGG 2 cut(s) 200, 246
CseI GACGC 1 cut(s) 122
Csp6I GTAC 1 cut(s) 457
CspAI ACCGGT 1 cut(s) 307
CviAII CATG 2 cut(s) 397, 688
CviQI GTAC 1 cut(s) 457
DdeI CTNAG 1 cut(s) 652
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
EaeI YGGCCR 1 cut(s) 709
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 2 cut(s) 365, 478
Eco24I GRGCYC 1 cut(s) 231
Eco53kI GAGCTC 1 cut(s) 229
Eco88I CYCGRG 3 cut(s) 200, 246, 500
Eco91I GGTNACC 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 666
EcoO65I GGTNACC 1 cut(s) 209
EcoRI GAATTC 1 cut(s) 602
EcoRII CCWGG 1 cut(s) 668
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 2 cut(s) 400, 691
FaiI YATR 3 cut(s) 398, 681, 689
FalI AAGNNNNNCTT 4 cut(s) 53, 85, 590, 622
FaqI GGGAC 1 cut(s) 264
FatI CATG 2 cut(s) 396, 687
Fnu4HI GCNGC 2 cut(s) 166, 302
FokI GGATG 1 cut(s) 647
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 2 cut(s) 166, 302
FspBI CTAG 2 cut(s) 555, 760
GlaI GCGC 1 cut(s) 375
GluI GCNGC 2 cut(s) 166, 302
GsaI CCCAGC 2 cut(s) 589, 724
HaeIII GGCC 9 cut(s) 129, 168, 359, 365, 395, 449, 478, 668, 711
HapII CCGG 7 cut(s) 201, 214, 247, 259, 308, 350, 511
HgaI GACGC 1 cut(s) 122
HhaI GCGC 1 cut(s) 376
Hin1I GRCGYC 3 cut(s) 114, 342, 507
Hin1II CATG 2 cut(s) 400, 691
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HindIII AAGCTT 1 cut(s) 382
HinfI GANTC 3 cut(s) 23, 252, 738
HpaI GTTAAC 1 cut(s) 579
HpaII CCGG 7 cut(s) 201, 214, 247, 259, 308, 350, 511
HphI GGTGA 1 cut(s) 203
Hpy166II GTNNAC 3 cut(s) 435, 579, 697
Hpy188I TCNGA 2 cut(s) 16, 655
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 3 cut(s) 435, 579, 697
Hpy99I CGWCG 6 cut(s) 20, 23, 116, 269, 344, 347
HpyAV CCTTC 3 cut(s) 409, 592, 656
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 3 cut(s) 342, 459, 507
HpyCH4V TGCA 3 cut(s) 279, 545, 754
HpyF10VI GCNNNNNNNGC 3 cut(s) 298, 371, 717
HpyF3I CTNAG 1 cut(s) 652
HpySE526I ACGT 3 cut(s) 342, 459, 507
Hsp92I GRCGYC 3 cut(s) 114, 342, 507
Hsp92II CATG 2 cut(s) 400, 691
HspAI GCGC 1 cut(s) 374
KspAI GTTAAC 1 cut(s) 579
Kzo9I GATC 1 cut(s) 216
MaeI CTAG 2 cut(s) 555, 760
MaeII ACGT 3 cut(s) 342, 459, 507
MaeIII GTNAC 1 cut(s) 209
MalI GATC 1 cut(s) 218
MbiI CCGCTC 1 cut(s) 301
MboI GATC 1 cut(s) 216
MboII GAAGA 1 cut(s) 34
MfeI CAATTG 1 cut(s) 314
MhlI GDGCHC 2 cut(s) 231, 294
MluCI AATT 4 cut(s) 95, 314, 602, 715
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 39
MroXI GAANNNNTTC 2 cut(s) 602, 606
MseI TTAA 2 cut(s) 98, 578
MslI CAYNNNNRTG 1 cut(s) 692
MspA1I CMGCKG 1 cut(s) 585
MspI CCGG 7 cut(s) 201, 214, 247, 259, 308, 350, 511
MspR9I CCNGG 6 cut(s) 201, 202, 247, 248, 259, 670
MunI CAATTG 1 cut(s) 314
Mva1269I GAATGC 1 cut(s) 281
MvaI CCWGG 1 cut(s) 670
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 3 cut(s) 298, 371, 717
NciI CCSGG 5 cut(s) 201, 202, 247, 248, 259
NdeII GATC 1 cut(s) 216
NlaIII CATG 2 cut(s) 400, 691
NlaIV GGNNCC 3 cut(s) 130, 169, 667
NmeAIII GCCGAG 2 cut(s) 385, 687
NmuCI GTSAC 1 cut(s) 209
NspV TTCGAA 1 cut(s) 610
PaeR7I CTCGAG 1 cut(s) 500
PceI AGGCCT 2 cut(s) 365, 478
PcsI WCGNNNNNNNCGW 4 cut(s) 108, 261, 294, 351
PctI GAATGC 1 cut(s) 281
PdmI GAANNNNTTC 2 cut(s) 602, 606
PfeI GAWTC 2 cut(s) 23, 738
PflFI GACNNNGTC 1 cut(s) 341
PinAI ACCGGT 1 cut(s) 307
PkrI GCNGC 2 cut(s) 167, 303
PleI GAGTC 1 cut(s) 246
PpsI GAGTC 1 cut(s) 246
Ppu21I YACGTR 1 cut(s) 460
PshBI ATTAAT 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 668
PspEI GGTNACC 1 cut(s) 209
PspFI CCCAGC 2 cut(s) 585, 720
PspGI CCWGG 1 cut(s) 668
PspN4I GGNNCC 3 cut(s) 130, 169, 667
PspPI GGNCC 3 cut(s) 128, 167, 666
PstI CTGCAG 1 cut(s) 756
PstNI CAGNNNCTG 1 cut(s) 566
PsyI GACNNNGTC 1 cut(s) 341
PvuII CAGCTG 1 cut(s) 585
RsaI GTAC 1 cut(s) 458
RsaNI GTAC 1 cut(s) 457
RseI CAYNNNNRTG 1 cut(s) 692
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 2 cut(s) 98, 578
SatI GCNGC 2 cut(s) 166, 302
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 3 cut(s) 128, 167, 666
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 6 cut(s) 201, 202, 247, 248, 259, 670
SduI GDGCHC 2 cut(s) 231, 294
SfcI CTRYAG 1 cut(s) 752
Sfr274I CTCGAG 1 cut(s) 500
SfuI TTCGAA 1 cut(s) 610
SlaI CTCGAG 1 cut(s) 500
SmaI CCCGGG 2 cut(s) 202, 248
SmiMI CAYNNNNRTG 1 cut(s) 692
SmlI CTYRAG 2 cut(s) 59, 500
SmoI CTYRAG 2 cut(s) 59, 500
Sse9I AATT 4 cut(s) 95, 314, 602, 715
SseBI AGGCCT 2 cut(s) 365, 478
SsiI CCGC 3 cut(s) 165, 301, 329
SspI AATATT 1 cut(s) 496
SspMI CTAG 2 cut(s) 555, 760
SstI GAGCTC 1 cut(s) 231
StuI AGGCCT 2 cut(s) 365, 478
StyD4I CCNGG 6 cut(s) 199, 200, 245, 246, 257, 668
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 3 cut(s) 345, 462, 510
TaqI TCGA 6 cut(s) 102, 242, 264, 354, 501, 610
TasI AATT 4 cut(s) 95, 314, 602, 715
TauI GCSGC 2 cut(s) 168, 304
TfiI GAWTC 2 cut(s) 23, 738
Tru1I TTAA 2 cut(s) 98, 578
Tru9I TTAA 2 cut(s) 98, 578
TscAI CASTG 2 cut(s) 331, 552
TseFI GTSAC 1 cut(s) 209
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 112
TspGWI ACGGA 2 cut(s) 189, 452
TspMI CCCGGG 2 cut(s) 200, 246
TspRI CASTG 2 cut(s) 331, 552
Tth111I GACNNNGTC 1 cut(s) 341
VspI ATTAAT 1 cut(s) 98
XapI RAATTY 1 cut(s) 602
XhoI CTCGAG 1 cut(s) 500
XmaI CCCGGG 2 cut(s) 200, 246
XmnI GAANNNNTTC 2 cut(s) 602, 606
XspI CTAG 2 cut(s) 555, 760
ZraI GACGTC 2 cut(s) 343, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.