Rw7G021250

Gamma-glutamyl cyclotransferase, AIG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007085
Physical Location & Seq
Reverse (-)
24510245 .. 24510778
534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G021250.1

Sequence Viewer

Length: 534 bp
ATGGCTAGCATTTCCGGCGACGAATCCAAACCCCACCTCATCTTCTCCTACGGCACACTCAAGCAAGGCTTCCGCAACCACAAGCTCACCAAGGAATTAATCGACCAAGACGACGCCGTTTTCATCGGCCCCTGCAGTACCCTCCACACCTACCCACTCGTCTGCGGCCCCCACGGATTGCCCTACCTCATCAACCTCCCCAGGTCGGGTCACCGGATCAGGGGCGAGCTCTACGCCGTTTCGACCCGGGGACTCGCCTGGTTCGACGAACTGGAAGGCACCTCCATCGGGCACTACGAGCGGCTCCCGGTACAATTGACGGTCAATGCGGACAGAGACGACGTCGTTCCGGTCGAGGCCGAGGCCTATTTCGCGCACAGAAGCTTCGGAGAGGCCATGTGGGAGAGGAACGGGAAGGCGGGTTTGAGCGAGTTCACGGAGAATGAGACCAAAGGGTACCTGAAAAGACAGGATAGGCCTGATCAGGGTCTCAATATTCTCGAGGACGTGCGGCATTTTGTGAATGGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

177

Amino Acids

19.96

Weight (kDa)

5.6

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 14 - 127 1.6e-17 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 345
Acc65I GGTACC 1 cut(s) 456
AccB1I GGYRCC 2 cut(s) 278, 456
AccBSI CCGCTC 1 cut(s) 301
AccII CGCG 1 cut(s) 374
AciI CCGC 6 cut(s) 73, 165, 301, 329, 419, 511
AclWI GGATC 1 cut(s) 224
AcyI GRCGYC 2 cut(s) 114, 342
AfaI GTAC 3 cut(s) 139, 312, 458
AfiI CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
AjiI CACGTC 1 cut(s) 508
AjnI CCWGG 2 cut(s) 200, 257
AluBI AGCT 3 cut(s) 85, 229, 384
AluI AGCT 3 cut(s) 85, 229, 384
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 3 cut(s) 330, 440, 494
AlwI GGATC 1 cut(s) 224
Ama87I CYCGRG 2 cut(s) 246, 500
AoxI GGCC 6 cut(s) 127, 166, 357, 363, 393, 476
AseI ATTAAT 1 cut(s) 98
Asp718I GGTACC 1 cut(s) 456
AspLEI GCGC 1 cut(s) 376
AspS9I GGNCC 2 cut(s) 128, 167
AsuC2I CCSGG 3 cut(s) 247, 248, 308
AsuHPI GGTGA 2 cut(s) 79, 203
AsuNHI GCTAGC 1 cut(s) 5
AvaI CYCGRG 2 cut(s) 246, 500
BaeGI GKGCMC 1 cut(s) 294
BanI GGYRCC 2 cut(s) 278, 456
BanII GRGCYC 1 cut(s) 231
Bbv12I GWGCWC 1 cut(s) 231
BccI CCATC 1 cut(s) 293
BceAI ACGGC 3 cut(s) 67, 101, 221
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BciT130I CCWGG 2 cut(s) 202, 259
BclI TGATCA 1 cut(s) 481
BcnI CCSGG 3 cut(s) 247, 248, 308
BcoDI GTCTC 3 cut(s) 330, 440, 494
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 133
BisI GCNGC 3 cut(s) 166, 302, 512
BlsI GCNGC 3 cut(s) 167, 303, 513
Bme1390I CCNGG 5 cut(s) 202, 247, 248, 259, 308
BmeT110I CYCGRG 2 cut(s) 246, 500
BmgBI CACGTC 1 cut(s) 508
BmgT120I GGNCC 2 cut(s) 128, 167
BmiI GGNNCC 5 cut(s) 130, 169, 280, 305, 458
BmrFI CCNGG 5 cut(s) 202, 247, 248, 259, 308
BmtI GCTAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 3 cut(s) 247, 248, 308
BsaHI GRCGYC 2 cut(s) 114, 342
BsaI GGTCTC 2 cut(s) 440, 494
BsaJI CCNNGG 6 cut(s) 90, 172, 200, 246, 247, 360
BsaWI WCCGGW 2 cut(s) 213, 349
Bsc4I CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
Bse1I ACTGG 1 cut(s) 276
BseBI CCWGG 2 cut(s) 202, 259
BseDI CCNNGG 6 cut(s) 90, 172, 200, 246, 247, 360
BseLI CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
BseNI ACTGG 1 cut(s) 276
BseSI GKGCMC 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 374
Bsh1285I CGRYCG 1 cut(s) 354
BshFI GGCC 6 cut(s) 129, 168, 359, 365, 395, 478
BshNI GGYRCC 2 cut(s) 278, 456
BsiEI CGRYCG 1 cut(s) 354
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 2 cut(s) 246, 500
BsiSI CCGG 5 cut(s) 15, 214, 247, 308, 350
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 5 cut(s) 205, 206, 220, 288, 485
BsmAI GTCTC 3 cut(s) 330, 440, 494
BsmBI CGTCTC 1 cut(s) 330
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 6 cut(s) 129, 168, 359, 365, 395, 478
Bso31I GGTCTC 2 cut(s) 440, 494
BsoBI CYCGRG 2 cut(s) 246, 500
Bsp1286I GDGCHC 2 cut(s) 231, 294
Bsp143I GATC 2 cut(s) 216, 481
BspACI CCGC 6 cut(s) 73, 165, 301, 329, 419, 511
BspANI GGCC 6 cut(s) 129, 168, 359, 365, 395, 478
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 5 cut(s) 130, 169, 280, 305, 458
BspMAI CTGCAG 1 cut(s) 137
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 224
BspT107I GGYRCC 2 cut(s) 278, 456
BspTNI GGTCTC 2 cut(s) 440, 494
BsrBI CCGCTC 1 cut(s) 301
BsrI ACTGG 1 cut(s) 276
BssECI CCNNGG 6 cut(s) 90, 172, 200, 246, 247, 360
BssMI GATC 2 cut(s) 216, 481
BssNI GRCGYC 2 cut(s) 114, 342
BssT1I CCWWGG 1 cut(s) 90
Bst2UI CCWGG 2 cut(s) 202, 259
Bst4CI ACNGT 1 cut(s) 322
BstACI GRCGYC 2 cut(s) 114, 342
BstC8I GCNNGC 2 cut(s) 7, 227
BstDSI CCRYGG 1 cut(s) 172
BstEII GGTNACC 1 cut(s) 209
BstFNI CGCG 1 cut(s) 374
BstHHI GCGC 1 cut(s) 376
BstKTI GATC 2 cut(s) 219, 484
BstMAI GTCTC 3 cut(s) 330, 440, 494
BstMBI GATC 2 cut(s) 216, 481
BstMCI CGRYCG 1 cut(s) 354
BstMWI GCNNNNNNNGC 3 cut(s) 15, 298, 371
BstNI CCWGG 2 cut(s) 202, 259
BstPI GGTNACC 1 cut(s) 209
BstSCI CCNGG 5 cut(s) 200, 245, 246, 257, 306
BstSFI CTRYAG 1 cut(s) 133
BstSLI GKGCMC 1 cut(s) 294
BstUI CGCG 1 cut(s) 374
BsuRI GGCC 6 cut(s) 129, 168, 359, 365, 395, 478
BtgI CCRYGG 1 cut(s) 172
BtrI CACGTC 1 cut(s) 508
Cac8I GCNNGC 2 cut(s) 7, 227
CfoI GCGC 1 cut(s) 376
Cfr13I GGNCC 2 cut(s) 128, 167
Cfr9I CCCGGG 1 cut(s) 246
CseI GACGC 1 cut(s) 122
Csp6I GTAC 3 cut(s) 138, 311, 457
CviAII CATG 1 cut(s) 397
CviQI GTAC 3 cut(s) 138, 311, 457
DpnI GATC 2 cut(s) 218, 483
DpnII GATC 2 cut(s) 216, 481
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 2 cut(s) 365, 478
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 2 cut(s) 440, 494
Eco53kI GAGCTC 1 cut(s) 229
Eco88I CYCGRG 2 cut(s) 246, 500
Eco91I GGTNACC 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 209
EcoRII CCWGG 2 cut(s) 200, 257
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 90
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 1 cut(s) 400
FaiI YATR 1 cut(s) 398
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 1 cut(s) 264
FatI CATG 1 cut(s) 396
FauI CCCGC 1 cut(s) 412
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 3 cut(s) 166, 302, 512
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 3 cut(s) 166, 302, 512
FspBI CTAG 1 cut(s) 6
GlaI GCGC 1 cut(s) 375
GluI GCNGC 3 cut(s) 166, 302, 512
HaeIII GGCC 6 cut(s) 129, 168, 359, 365, 395, 478
HapII CCGG 5 cut(s) 15, 214, 247, 308, 350
HgaI GACGC 1 cut(s) 122
HhaI GCGC 1 cut(s) 376
Hin1I GRCGYC 2 cut(s) 114, 342
Hin1II CATG 1 cut(s) 400
Hin6I GCGC 1 cut(s) 374
HinP1I GCGC 1 cut(s) 374
HindIII AAGCTT 1 cut(s) 382
HinfI GANTC 2 cut(s) 23, 252
HpaII CCGG 5 cut(s) 15, 214, 247, 308, 350
HphI GGTGA 2 cut(s) 79, 203
Hpy166II GTNNAC 1 cut(s) 435
Hpy188I TCNGA 1 cut(s) 389
Hpy188III TCNNGA 1 cut(s) 500
Hpy8I GTNNAC 1 cut(s) 435
Hpy99I CGWCG 5 cut(s) 23, 116, 269, 344, 347
HpyAV CCTTC 2 cut(s) 269, 409
HpyCH4III ACNGT 1 cut(s) 322
HpyCH4IV ACGT 2 cut(s) 342, 507
HpyCH4V TGCA 1 cut(s) 135
HpyF10VI GCNNNNNNNGC 3 cut(s) 15, 298, 371
HpySE526I ACGT 2 cut(s) 342, 507
Hsp92I GRCGYC 2 cut(s) 114, 342
Hsp92II CATG 1 cut(s) 400
HspAI GCGC 1 cut(s) 374
KpnI GGTACC 1 cut(s) 460
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 2 cut(s) 216, 481
LmnI GCTCC 1 cut(s) 309
MaeI CTAG 1 cut(s) 6
MaeII ACGT 2 cut(s) 342, 507
MaeIII GTNAC 1 cut(s) 209
MalI GATC 2 cut(s) 218, 483
MbiI CCGCTC 1 cut(s) 301
MboI GATC 2 cut(s) 216, 481
MboII GAAGA 1 cut(s) 34
MfeI CAATTG 1 cut(s) 314
MhlI GDGCHC 2 cut(s) 231, 294
MluCI AATT 2 cut(s) 95, 314
MlyI GAGTC 1 cut(s) 246
MseI TTAA 1 cut(s) 98
MspI CCGG 5 cut(s) 15, 214, 247, 308, 350
MspR9I CCNGG 5 cut(s) 202, 247, 248, 259, 308
MunI CAATTG 1 cut(s) 314
MvaI CCWGG 2 cut(s) 202, 259
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 3 cut(s) 15, 298, 371
NciI CCSGG 3 cut(s) 247, 248, 308
NdeII GATC 2 cut(s) 216, 481
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 5 cut(s) 130, 169, 280, 305, 458
NmeAIII GCCGAG 1 cut(s) 385
NmuCI GTSAC 1 cut(s) 209
PaeR7I CTCGAG 1 cut(s) 500
PceI AGGCCT 2 cut(s) 365, 478
PcsI WCGNNNNNNNCGW 4 cut(s) 108, 261, 294, 351
PfeI GAWTC 1 cut(s) 23
PflFI GACNNNGTC 1 cut(s) 341
PkrI GCNGC 3 cut(s) 167, 303, 513
PleI GAGTC 1 cut(s) 246
PpsI GAGTC 1 cut(s) 246
PshBI ATTAAT 1 cut(s) 98
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 200, 257
PspEI GGTNACC 1 cut(s) 209
PspGI CCWGG 2 cut(s) 200, 257
PspN4I GGNNCC 5 cut(s) 130, 169, 280, 305, 458
PspPI GGNCC 2 cut(s) 128, 167
PstI CTGCAG 1 cut(s) 137
PsyI GACNNNGTC 1 cut(s) 341
RsaI GTAC 3 cut(s) 139, 312, 458
RsaNI GTAC 3 cut(s) 138, 311, 457
SacI GAGCTC 1 cut(s) 231
SaqAI TTAA 1 cut(s) 98
SatI GCNGC 3 cut(s) 166, 302, 512
Sau3AI GATC 2 cut(s) 216, 481
Sau96I GGNCC 2 cut(s) 128, 167
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 5 cut(s) 202, 247, 248, 259, 308
SduI GDGCHC 2 cut(s) 231, 294
SfcI CTRYAG 1 cut(s) 133
Sfr274I CTCGAG 1 cut(s) 500
SlaI CTCGAG 1 cut(s) 500
SmaI CCCGGG 1 cut(s) 248
SmlI CTYRAG 2 cut(s) 59, 500
SmoI CTYRAG 2 cut(s) 59, 500
Sse9I AATT 2 cut(s) 95, 314
SseBI AGGCCT 2 cut(s) 365, 478
SsiI CCGC 6 cut(s) 73, 165, 301, 329, 419, 511
SspI AATATT 1 cut(s) 496
SspMI CTAG 1 cut(s) 6
SstI GAGCTC 1 cut(s) 231
StuI AGGCCT 2 cut(s) 365, 478
StyD4I CCNGG 5 cut(s) 200, 245, 246, 257, 306
StyI CCWWGG 1 cut(s) 90
TaaI ACNGT 1 cut(s) 322
TaiI ACGT 2 cut(s) 345, 510
TaqI TCGA 5 cut(s) 102, 242, 264, 354, 501
TasI AATT 2 cut(s) 95, 314
TauI GCSGC 3 cut(s) 168, 304, 514
TfiI GAWTC 1 cut(s) 23
Tru1I TTAA 1 cut(s) 98
Tru9I TTAA 1 cut(s) 98
TseFI GTSAC 1 cut(s) 209
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 112
TspGWI ACGGA 2 cut(s) 189, 452
TspMI CCCGGG 1 cut(s) 246
Tth111I GACNNNGTC 1 cut(s) 341
VspI ATTAAT 1 cut(s) 98
XhoI CTCGAG 1 cut(s) 500
XmaI CCCGGG 1 cut(s) 246
XspI CTAG 1 cut(s) 6
ZraI GACGTC 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.