FvH4_5g22190

Gamma-glutamyl cyclotransferase, AIG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007085
Physical Location & Seq
Reverse (-)
13522225 .. 13524796
2572 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g22190.t1

Sequence Viewer

Length: 534 bp
ATGGCTGACATTTCCGGCGACGACTCGAAGCCCCACCTGATCTTCTCCTACGGCACACTCAAACAAGGCTTCCACAACCACAAGCTCACCAAGGAGCTCATCTCCCAAAACGACGCCGTCTTCATCGGCCCCTACATTACCCGCCACGCCTACCCCCTCGTCTGTGGCCCCCACGGCCTCCCCTACCTCATCAACCTCCCCGGGTCGGGTCGCCGCATCCGGGGCGAGCTCTACGCGGTCTCCGCCGTCGGCCTCGCCCGCTTCGACGACCTCGAAGGCACCTCCATCGGCCACTACGAGCGCCTCCGGCTCCAATTGCTCGTCAATGCCGACAAAGACGGCGCCGTTCCCGTCGACGCCGAGGCCTACTTCGCCCACCGGAGCTTCGGCGAGGCCATGTGGGAGAGGAACGGCAAGGAGGGCCTGACGGAGTTTACGGAGAACGAGGCCAAGAGGTATGTGAAAAGACAGGATAGGCCTCATCAGGGGAGGAATATTATCGAGGACGTGCGGCGGTTTGTGAATGGGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

178

Amino Acids

19.98

Weight (kDa)

6.71

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 14 - 127 1.2e-15 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 278, 341
AccI GTMKAC 1 cut(s) 354
AccII CGCG 1 cut(s) 236
AciI CCGC 7 cut(s) 142, 214, 236, 243, 259, 511, 514
AcoI YGGCCR 1 cut(s) 289
AcyI GRCGYC 3 cut(s) 114, 342, 357
AfiI CCNNNNNNNGG 4 cut(s) 205, 206, 220, 485
AjiI CACGTC 1 cut(s) 508
AluBI AGCT 4 cut(s) 85, 97, 229, 384
AluI AGCT 4 cut(s) 85, 97, 229, 384
Alw21I GWGCWC 2 cut(s) 99, 231
Alw26I GTCTC 1 cut(s) 244
Ama87I CYCGRG 1 cut(s) 200
AspLEI GCGC 2 cut(s) 303, 344
AspS9I GGNCC 3 cut(s) 128, 167, 421
AsuC2I CCSGG 3 cut(s) 201, 202, 221
AsuHPI GGTGA 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 200
BanI GGYRCC 2 cut(s) 278, 341
BanII GRGCYC 2 cut(s) 99, 231
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 2 cut(s) 99, 231
BccI CCATC 1 cut(s) 293
BceAI ACGGC 7 cut(s) 67, 101, 190, 230, 329, 355, 427
BcgI CGANNNNNNTGC 2 cut(s) 268, 302
BcnI CCSGG 3 cut(s) 201, 202, 221
BcoDI GTCTC 1 cut(s) 244
BfoI RGCGCY 2 cut(s) 304, 345
BglI GCCNNNNNGGC 1 cut(s) 174
BisI GCNGC 2 cut(s) 214, 512
BlsI GCNGC 2 cut(s) 215, 513
Bme1390I CCNGG 3 cut(s) 201, 202, 221
BmeT110I CYCGRG 1 cut(s) 200
BmgBI CACGTC 1 cut(s) 508
BmgT120I GGNCC 3 cut(s) 128, 167, 421
BmiI GGNNCC 5 cut(s) 130, 169, 280, 311, 343
BmrFI CCNGG 3 cut(s) 201, 202, 221
BmsI GCATC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 112
BplI GAGNNNNNCTC 2 cut(s) 86, 118
BpuMI CCSGG 3 cut(s) 201, 202, 221
BsaHI GRCGYC 3 cut(s) 114, 342, 357
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 6 cut(s) 90, 172, 199, 200, 220, 360
BsaWI WCCGGW 1 cut(s) 378
BsaXI ACNNNNNCTCC 2 cut(s) 224, 254
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 206, 220, 485
BseDI CCNNGG 6 cut(s) 90, 172, 199, 200, 220, 360
BseGI GGATG 1 cut(s) 216
BseLI CCNNNNNNNGG 4 cut(s) 205, 206, 220, 485
Bsh1236I CGCG 1 cut(s) 236
BshNI GGYRCC 2 cut(s) 278, 341
BsiHKAI GWGCWC 2 cut(s) 99, 231
BsiHKCI CYCGRG 1 cut(s) 200
BsiSI CCGG 5 cut(s) 15, 201, 220, 307, 379
BslI CCNNNNNNNGG 4 cut(s) 205, 206, 220, 485
BsmAI GTCTC 1 cut(s) 244
Bso31I GGTCTC 1 cut(s) 244
BsoBI CYCGRG 1 cut(s) 200
Bsp1286I GDGCHC 2 cut(s) 99, 231
Bsp143I GATC 1 cut(s) 39
BspACI CCGC 7 cut(s) 142, 214, 236, 243, 259, 511, 514
BspFNI CGCG 1 cut(s) 236
BspLI GGNNCC 5 cut(s) 130, 169, 280, 311, 343
BspT107I GGYRCC 2 cut(s) 278, 341
BspTNI GGTCTC 1 cut(s) 244
BssECI CCNNGG 6 cut(s) 90, 172, 199, 200, 220, 360
BssMI GATC 1 cut(s) 39
BssNI GRCGYC 3 cut(s) 114, 342, 357
BssT1I CCWWGG 1 cut(s) 90
BstACI GRCGYC 3 cut(s) 114, 342, 357
BstC8I GCNNGC 2 cut(s) 227, 259
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 1 cut(s) 216
BstFNI CGCG 1 cut(s) 236
BstH2I RGCGCY 2 cut(s) 304, 345
BstHHI GCGC 2 cut(s) 303, 344
BstKTI GATC 1 cut(s) 42
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 1 cut(s) 39
BstMWI GCNNNNNNNGC 8 cut(s) 174, 222, 242, 258, 307, 316, 371, 420
BstSCI CCNGG 3 cut(s) 199, 200, 219
BstUI CGCG 1 cut(s) 236
BstV2I GAAGAC 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 172
BtrI CACGTC 1 cut(s) 508
BtsCI GGATG 1 cut(s) 216
Cac8I GCNNGC 2 cut(s) 227, 259
CfoI GCGC 2 cut(s) 303, 344
Cfr13I GGNCC 3 cut(s) 128, 167, 421
Cfr9I CCCGGG 1 cut(s) 200
CseI GACGC 2 cut(s) 122, 365
CviAII CATG 1 cut(s) 397
DinI GGCGCC 1 cut(s) 343
DpnI GATC 1 cut(s) 41
DpnII GATC 1 cut(s) 39
EaeI YGGCCR 1 cut(s) 289
EciI GGCGGA 1 cut(s) 232
Ecl136II GAGCTC 2 cut(s) 97, 229
Eco130I CCWWGG 1 cut(s) 90
Eco147I AGGCCT 2 cut(s) 365, 478
Eco24I GRGCYC 2 cut(s) 99, 231
Eco31I GGTCTC 1 cut(s) 244
Eco53kI GAGCTC 2 cut(s) 97, 229
Eco88I CYCGRG 1 cut(s) 200
EcoICRI GAGCTC 2 cut(s) 97, 229
EcoO109I RGGNCCY 1 cut(s) 421
EcoT14I CCWWGG 1 cut(s) 90
EcoT38I GRGCYC 2 cut(s) 99, 231
EgeI GGCGCC 1 cut(s) 343
EheI GGCGCC 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 90
FaeI CATG 1 cut(s) 400
FaiI YATR 2 cut(s) 398, 459
FatI CATG 1 cut(s) 396
FauI CCCGC 2 cut(s) 149, 266
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 2 cut(s) 214, 512
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 2 cut(s) 99, 231
Fsp4HI GCNGC 2 cut(s) 214, 512
GlaI GCGC 2 cut(s) 302, 343
GluI GCNGC 2 cut(s) 214, 512
HaeII RGCGCY 2 cut(s) 304, 345
HapII CCGG 5 cut(s) 15, 201, 220, 307, 379
HgaI GACGC 2 cut(s) 122, 365
HhaI GCGC 2 cut(s) 303, 344
Hin1I GRCGYC 3 cut(s) 114, 342, 357
Hin1II CATG 1 cut(s) 400
Hin6I GCGC 2 cut(s) 301, 342
HinP1I GCGC 2 cut(s) 301, 342
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 1 cut(s) 23
HpaII CCGG 5 cut(s) 15, 201, 220, 307, 379
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 2 cut(s) 355, 435
Hpy8I GTNNAC 2 cut(s) 355, 435
Hpy99I CGWCG 6 cut(s) 23, 116, 251, 269, 356, 359
HpyAV CCTTC 1 cut(s) 269
HpyCH4IV ACGT 1 cut(s) 507
HpyF10VI GCNNNNNNNGC 8 cut(s) 174, 222, 242, 258, 307, 316, 371, 420
HpySE526I ACGT 1 cut(s) 507
Hsp92I GRCGYC 3 cut(s) 114, 342, 357
Hsp92II CATG 1 cut(s) 400
HspAI GCGC 2 cut(s) 301, 342
KasI GGCGCC 1 cut(s) 341
Kzo9I GATC 1 cut(s) 39
LmnI GCTCC 3 cut(s) 94, 315, 381
LpnPI CCDG 9 cut(s) 28, 50, 214, 233, 320, 392, 437, 455, 470
LweI GCATC 1 cut(s) 225
MaeII ACGT 1 cut(s) 507
MalI GATC 1 cut(s) 41
MboI GATC 1 cut(s) 39
MboII GAAGA 2 cut(s) 34, 112
MfeI CAATTG 1 cut(s) 314
MhlI GDGCHC 2 cut(s) 99, 231
MluCI AATT 1 cut(s) 314
Mly113I GGCGCC 1 cut(s) 342
MlyI GAGTC 1 cut(s) 17
MspI CCGG 5 cut(s) 15, 201, 220, 307, 379
MspR9I CCNGG 3 cut(s) 201, 202, 221
MunI CAATTG 1 cut(s) 314
MvnI CGCG 1 cut(s) 236
MwoI GCNNNNNNNGC 8 cut(s) 174, 222, 242, 258, 307, 316, 371, 420
NarI GGCGCC 1 cut(s) 342
NciI CCSGG 3 cut(s) 201, 202, 221
NdeII GATC 1 cut(s) 39
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 5 cut(s) 130, 169, 280, 311, 343
NmeAIII GCCGAG 1 cut(s) 385
PceI AGGCCT 2 cut(s) 365, 478
PcsI WCGNNNNNNNCGW 4 cut(s) 261, 270, 294, 327
PflFI GACNNNGTC 1 cut(s) 116
PkrI GCNGC 2 cut(s) 215, 513
PleI GAGTC 1 cut(s) 17
PluTI GGCGCC 1 cut(s) 345
PpsI GAGTC 1 cut(s) 17
Psp124BI GAGCTC 2 cut(s) 99, 231
PspN4I GGNNCC 5 cut(s) 130, 169, 280, 311, 343
PspPI GGNCC 3 cut(s) 128, 167, 421
PsyI GACNNNGTC 1 cut(s) 116
SacI GAGCTC 2 cut(s) 99, 231
SalI GTCGAC 1 cut(s) 353
SatI GCNGC 2 cut(s) 214, 512
Sau3AI GATC 1 cut(s) 39
Sau96I GGNCC 3 cut(s) 128, 167, 421
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 3 cut(s) 201, 202, 221
SduI GDGCHC 2 cut(s) 99, 231
SfaNI GCATC 1 cut(s) 225
SfiI GGCCNNNNNGGCC 1 cut(s) 174
SfoI GGCGCC 1 cut(s) 343
SgrDI CGTCGACG 1 cut(s) 353
SmaI CCCGGG 1 cut(s) 202
Sse9I AATT 1 cut(s) 314
SseBI AGGCCT 2 cut(s) 365, 478
SsiI CCGC 7 cut(s) 142, 214, 236, 243, 259, 511, 514
SspDI GGCGCC 1 cut(s) 341
SspI AATATT 1 cut(s) 496
SstI GAGCTC 2 cut(s) 99, 231
StuI AGGCCT 2 cut(s) 365, 478
StyD4I CCNGG 3 cut(s) 199, 200, 219
StyI CCWWGG 1 cut(s) 90
TaiI ACGT 1 cut(s) 510
TaqI TCGA 5 cut(s) 26, 264, 273, 354, 501
TasI AATT 1 cut(s) 314
TauI GCSGC 2 cut(s) 216, 514
TspDTI ATGAA 1 cut(s) 112
TspGWI ACGGA 2 cut(s) 443, 452
TspMI CCCGGG 1 cut(s) 200
Tth111I GACNNNGTC 1 cut(s) 116
XmaI CCCGGG 1 cut(s) 200
XmiI GTMKAC 1 cut(s) 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.