Rh7DG256500

Gamma-glutamyl cyclotransferase, AIG2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0007085
Physical Location & Seq
Reverse (-)
26827281 .. 26827817
537 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG256500.1

Sequence Viewer

Length: 537 bp
ATGGCTGACATCTCCGACGACGACGAATCCAAACCCCACCTCATCTTCTCCTACGGCACACTCAAGCGAGGCTTCCACAACCACAAGCTGACCAAGGAATTAATCGACCAAGACGACGCCGTTTTCATCGGCCCCTATATTACCCTCCACACCTACCCCCTCGTCTGCGGCCCCCACGGCCTTCCCTACCTCATCAACCTCCCCGGGTCGGGTCACCGGATCAGGGGCGAGCTGTACGCCGTTTCGACCCGGGGACTCGCCCGGTTCGACGAGCTGGAATGCACCTCCATCGGGCACTACGAGCGGCTCCCGGTTCAATTGACGGTCAGTGCGGACAGAGACGACGTCGTTCCGGTCGAGGCCGAGGCCTATTTCGCGCACAGAAGCTTTGGGGAGGCCATGTGGGAGAGGAACGGGAAGGAGGGTTTGAGCGAGTTCACAGAGAATGAGGCGAAACGGTACGTGAAAAGACAGGATAGGCCTCATCAGGGTCGCAATATTCTCGAGGACGTCCGGCATTTTGTCAACGGAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GGACT

Protein Analysis

178

Amino Acids

20.32

Weight (kDa)

5.67

Isoelectric Point (pI)

39.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GGACT PF06094 15 - 128 2.2e-18 Gamma-glutamyl cyclotransferase, AIG2-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 348, 513
AccBSI CCGCTC 1 cut(s) 304
AccII CGCG 1 cut(s) 377
AciI CCGC 3 cut(s) 168, 304, 332
AclWI GGATC 1 cut(s) 227
AcyI GRCGYC 3 cut(s) 117, 345, 510
AfaI GTAC 2 cut(s) 236, 461
AfiI CCNNNNNNNGG 5 cut(s) 208, 209, 223, 291, 488
AgsI TTSAA 1 cut(s) 317
AluBI AGCT 4 cut(s) 88, 232, 274, 387
AluI AGCT 4 cut(s) 88, 232, 274, 387
Alw26I GTCTC 1 cut(s) 333
AlwI GGATC 1 cut(s) 227
Ama87I CYCGRG 3 cut(s) 203, 249, 503
AoxI GGCC 7 cut(s) 130, 169, 178, 360, 366, 396, 479
AseI ATTAAT 1 cut(s) 101
AspLEI GCGC 1 cut(s) 379
AspS9I GGNCC 2 cut(s) 131, 170
AsuC2I CCSGG 6 cut(s) 204, 205, 250, 251, 262, 311
AsuHPI GGTGA 1 cut(s) 206
AvaI CYCGRG 3 cut(s) 203, 249, 503
BaeGI GKGCMC 1 cut(s) 297
BccI CCATC 1 cut(s) 296
BceAI ACGGC 4 cut(s) 70, 104, 193, 224
BcgI CGANNNNNNTGC 4 cut(s) 271, 305, 484, 518
BcnI CCSGG 6 cut(s) 204, 205, 250, 251, 262, 311
BcoDI GTCTC 1 cut(s) 333
BglI GCCNNNNNGGC 1 cut(s) 177
BisI GCNGC 2 cut(s) 169, 305
BlsI GCNGC 2 cut(s) 170, 306
Bme1390I CCNGG 6 cut(s) 204, 205, 250, 251, 262, 311
BmeT110I CYCGRG 3 cut(s) 203, 249, 503
BmgT120I GGNCC 2 cut(s) 131, 170
BmiI GGNNCC 3 cut(s) 133, 172, 308
BmrFI CCNGG 6 cut(s) 204, 205, 250, 251, 262, 311
BpuEI CTTGAG 1 cut(s) 47
BpuMI CCSGG 6 cut(s) 204, 205, 250, 251, 262, 311
BsaAI YACGTR 1 cut(s) 463
BsaHI GRCGYC 3 cut(s) 117, 345, 510
BsaJI CCNNGG 7 cut(s) 93, 175, 202, 203, 249, 250, 363
BsaWI WCCGGW 2 cut(s) 216, 352
BsaXI ACNNNNNCTCC 2 cut(s) 386, 416
Bsc4I CCNNNNNNNGG 5 cut(s) 208, 209, 223, 291, 488
BseDI CCNNGG 7 cut(s) 93, 175, 202, 203, 249, 250, 363
BseLI CCNNNNNNNGG 5 cut(s) 208, 209, 223, 291, 488
BseSI GKGCMC 1 cut(s) 297
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 7 cut(s) 132, 171, 180, 362, 368, 398, 481
BsiEI CGRYCG 1 cut(s) 357
BsiHKCI CYCGRG 3 cut(s) 203, 249, 503
BsiSI CCGG 7 cut(s) 204, 217, 250, 262, 311, 353, 514
BslFI GGGAC 1 cut(s) 267
BslI CCNNNNNNNGG 5 cut(s) 208, 209, 223, 291, 488
BsmAI GTCTC 1 cut(s) 333
BsmBI CGTCTC 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 267
BsmI GAATGC 1 cut(s) 284
BsnI GGCC 7 cut(s) 132, 171, 180, 362, 368, 398, 481
BsoBI CYCGRG 3 cut(s) 203, 249, 503
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 1 cut(s) 219
BspACI CCGC 3 cut(s) 168, 304, 332
BspANI GGCC 7 cut(s) 132, 171, 180, 362, 368, 398, 481
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 3 cut(s) 133, 172, 308
BspPI GGATC 1 cut(s) 227
BsrBI CCGCTC 1 cut(s) 304
BssECI CCNNGG 7 cut(s) 93, 175, 202, 203, 249, 250, 363
BssMI GATC 1 cut(s) 219
BssNI GRCGYC 3 cut(s) 117, 345, 510
BssT1I CCWWGG 1 cut(s) 93
Bst4CI ACNGT 2 cut(s) 325, 459
BstACI GRCGYC 3 cut(s) 117, 345, 510
BstBAI YACGTR 1 cut(s) 463
BstC8I GCNNGC 1 cut(s) 230
BstDSI CCRYGG 1 cut(s) 175
BstEII GGTNACC 1 cut(s) 212
BstFNI CGCG 1 cut(s) 377
BstHHI GCGC 1 cut(s) 379
BstKTI GATC 1 cut(s) 222
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 1 cut(s) 219
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 3 cut(s) 177, 301, 374
BstPI GGTNACC 1 cut(s) 212
BstSCI CCNGG 6 cut(s) 202, 203, 248, 249, 260, 309
BstSLI GKGCMC 1 cut(s) 297
BstUI CGCG 1 cut(s) 377
BsuRI GGCC 7 cut(s) 132, 171, 180, 362, 368, 398, 481
BtgI CCRYGG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 230
CfoI GCGC 1 cut(s) 379
Cfr13I GGNCC 2 cut(s) 131, 170
Cfr9I CCCGGG 2 cut(s) 203, 249
CseI GACGC 1 cut(s) 125
Csp6I GTAC 2 cut(s) 235, 460
CviAII CATG 1 cut(s) 400
CviQI GTAC 2 cut(s) 235, 460
DpnI GATC 1 cut(s) 221
DpnII GATC 1 cut(s) 219
Eco130I CCWWGG 1 cut(s) 93
Eco147I AGGCCT 2 cut(s) 368, 481
Eco88I CYCGRG 3 cut(s) 203, 249, 503
Eco91I GGTNACC 1 cut(s) 212
EcoO65I GGTNACC 1 cut(s) 212
EcoT14I CCWWGG 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 93
Esp3I CGTCTC 1 cut(s) 333
FaeI CATG 1 cut(s) 403
FaiI YATR 2 cut(s) 138, 401
FalI AAGNNNNNCTT 2 cut(s) 56, 88
FaqI GGGAC 1 cut(s) 267
FatI CATG 1 cut(s) 399
Fnu4HI GCNGC 2 cut(s) 169, 305
Fsp4HI GCNGC 2 cut(s) 169, 305
GlaI GCGC 1 cut(s) 378
GluI GCNGC 2 cut(s) 169, 305
HaeIII GGCC 7 cut(s) 132, 171, 180, 362, 368, 398, 481
HapII CCGG 7 cut(s) 204, 217, 250, 262, 311, 353, 514
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 379
Hin1I GRCGYC 3 cut(s) 117, 345, 510
Hin1II CATG 1 cut(s) 403
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HindIII AAGCTT 1 cut(s) 385
HinfI GANTC 2 cut(s) 26, 255
HpaII CCGG 7 cut(s) 204, 217, 250, 262, 311, 353, 514
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 2 cut(s) 438, 526
Hpy188I TCNGA 1 cut(s) 16
Hpy188III TCNNGA 1 cut(s) 503
Hpy8I GTNNAC 2 cut(s) 438, 526
Hpy99I CGWCG 7 cut(s) 20, 23, 26, 119, 272, 347, 350
HpyAV CCTTC 2 cut(s) 191, 412
HpyCH4III ACNGT 2 cut(s) 325, 459
HpyCH4IV ACGT 3 cut(s) 345, 462, 510
HpyCH4V TGCA 1 cut(s) 282
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 301, 374
HpySE526I ACGT 3 cut(s) 345, 462, 510
Hsp92I GRCGYC 3 cut(s) 117, 345, 510
Hsp92II CATG 1 cut(s) 403
HspAI GCGC 1 cut(s) 377
Kzo9I GATC 1 cut(s) 219
LmnI GCTCC 1 cut(s) 312
MaeII ACGT 3 cut(s) 345, 462, 510
MaeIII GTNAC 1 cut(s) 212
MalI GATC 1 cut(s) 221
MbiI CCGCTC 1 cut(s) 304
MboI GATC 1 cut(s) 219
MboII GAAGA 1 cut(s) 37
MfeI CAATTG 1 cut(s) 317
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 2 cut(s) 98, 317
MlyI GAGTC 1 cut(s) 249
MmeI TCCRAC 1 cut(s) 39
MseI TTAA 1 cut(s) 101
MspI CCGG 7 cut(s) 204, 217, 250, 262, 311, 353, 514
MspR9I CCNGG 6 cut(s) 204, 205, 250, 251, 262, 311
MunI CAATTG 1 cut(s) 317
Mva1269I GAATGC 1 cut(s) 284
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 3 cut(s) 177, 301, 374
NciI CCSGG 6 cut(s) 204, 205, 250, 251, 262, 311
NdeII GATC 1 cut(s) 219
NlaIII CATG 1 cut(s) 403
NlaIV GGNNCC 3 cut(s) 133, 172, 308
NmeAIII GCCGAG 1 cut(s) 388
NmuCI GTSAC 1 cut(s) 212
PaeR7I CTCGAG 1 cut(s) 503
PceI AGGCCT 2 cut(s) 368, 481
PcsI WCGNNNNNNNCGW 4 cut(s) 111, 264, 297, 354
PctI GAATGC 1 cut(s) 284
PfeI GAWTC 1 cut(s) 26
PflFI GACNNNGTC 1 cut(s) 344
PkrI GCNGC 2 cut(s) 170, 306
PleI GAGTC 1 cut(s) 249
PpsI GAGTC 1 cut(s) 249
Ppu21I YACGTR 1 cut(s) 463
PshBI ATTAAT 1 cut(s) 101
PspEI GGTNACC 1 cut(s) 212
PspN4I GGNNCC 3 cut(s) 133, 172, 308
PspPI GGNCC 2 cut(s) 131, 170
PsyI GACNNNGTC 1 cut(s) 344
RsaI GTAC 2 cut(s) 236, 461
RsaNI GTAC 2 cut(s) 235, 460
SaqAI TTAA 1 cut(s) 101
SatI GCNGC 2 cut(s) 169, 305
Sau3AI GATC 1 cut(s) 219
Sau96I GGNCC 2 cut(s) 131, 170
SchI GAGTC 1 cut(s) 249
ScrFI CCNGG 6 cut(s) 204, 205, 250, 251, 262, 311
SduI GDGCHC 1 cut(s) 297
SfiI GGCCNNNNNGGCC 1 cut(s) 177
Sfr274I CTCGAG 1 cut(s) 503
SlaI CTCGAG 1 cut(s) 503
SmaI CCCGGG 2 cut(s) 205, 251
SmlI CTYRAG 2 cut(s) 62, 503
SmoI CTYRAG 2 cut(s) 62, 503
Sse9I AATT 2 cut(s) 98, 317
SseBI AGGCCT 2 cut(s) 368, 481
SsiI CCGC 3 cut(s) 168, 304, 332
SspI AATATT 1 cut(s) 499
StuI AGGCCT 2 cut(s) 368, 481
StyD4I CCNGG 6 cut(s) 202, 203, 248, 249, 260, 309
StyI CCWWGG 1 cut(s) 93
TaaI ACNGT 2 cut(s) 325, 459
TaiI ACGT 3 cut(s) 348, 465, 513
TaqI TCGA 5 cut(s) 105, 245, 267, 357, 504
TasI AATT 2 cut(s) 98, 317
TauI GCSGC 2 cut(s) 171, 307
TfiI GAWTC 1 cut(s) 26
Tru1I TTAA 1 cut(s) 101
Tru9I TTAA 1 cut(s) 101
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 115
TspMI CCCGGG 2 cut(s) 203, 249
TspRI CASTG 1 cut(s) 334
Tth111I GACNNNGTC 1 cut(s) 344
VspI ATTAAT 1 cut(s) 101
XhoI CTCGAG 1 cut(s) 503
XmaI CCCGGG 2 cut(s) 203, 249
ZraI GACGTC 2 cut(s) 346, 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.