AT5G47970

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008425
Physical Location & Seq
Forward (+)
19422203 .. 19425872
3670 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G47970.2

Sequence Viewer

Length: 1164 bp
ATGACAGTTTCAGAAGCTTATAGTCCACCTCTGTTCAGTATAGCCCCGATGATGGGATGGACAGACAATCATTACAGAACTCTAGCGCGTCTTATAACAAAACACGCATGGCTCTACACTGAAATGTTAGCTGCTGAAACCATTGTCTATCAAGAAGATAACCTGGACAGCTTTTTGGCATTCTCTCCAGACCAACATCCCATCGTTCTTCAAATTGGTGGAAGAAACTTGGAAAATTTGGCTAAAGCAACCAGACTTGCTAATGCTTACGCCTATGATGAAATTAATTTTAACTGTGGATGTCCTAGCCCAAAAGTAAGTGGACGAGGTTGTTTTGGTGCTCTTCTTATGCTTGACCCGAAGTTTGTTGGTGAGGCTATGTCTGTCATTGCAGCCAATACCAATGCAGCTGTCACTGTCAAATGTCGAATAGGTGTTGATGATCATGATTCATATAATGAGCTTTGTGATTTCATTCATATAGTTTCTTCATTATCTCCTACTAAGCATTTCATCATACATTCGCGGAAGGCATTACTGTCTGGACTTAGCCCGTCAGATAACCGTCGAATTCCGCCCTTAAAATACGAGTTTTTCTTTGCCCTTTTGCGTGATTTCCCGGACTTAAAGTTCACAATAAATGGAGGCATAAACTCTGTGGTTGAGGCAGATGCCGCATTAAGGTCTGGAGCTCATGGCGTTATGCTTGGGCGTGCTGTATACTACAATCCCTGGCACATTTTGGGACACGTCGATACTGTAGTTTACGGATCTCCAAGCAGTGGAATTACAAGGCGACAGGTTCTTGAAAAATATAAACTATATGGAGAGTCTGTTCTCGGGAAATATGGAAAAGGCCGACCAAATCTTCGAGATATAGTGAGGCCATTGATCAATTTGTTCCACTCGGAGTCTGGAAATGGCCAGTGGAAACGTAGAACCGATGCTGCTCTCTTGCACTGCACCACCTTACAATCATTCTTAGACGAAGTGTTACCAGCAATACCCGACTATGTCCTCGATTCATCTGCTGTCAAAGAGGCGACTGGACGTGAAGATCTATTTGCAGATGTACAACGTTTGTTGCCTCCTCCTTACGAAAAAGAATCTTTCAAAGCATTGGAAAGGATACCAACGAGACCTGTGATTCTTGATGAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

387

Amino Acids

43.15

Weight (kDa)

6.32

Isoelectric Point (pI)

42.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 14 - 318 4.1e-56 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 95
AccB7I CCANNNNNTGG 1 cut(s) 782
AccI GTMKAC 1 cut(s) 720
AccII CGCG 2 cut(s) 88, 526
AciI CCGC 3 cut(s) 526, 575, 675
AclI AACGTT 1 cut(s) 1078
AclWI GGATC 1 cut(s) 778
AcoI YGGCCR 1 cut(s) 922
AcsI RAATTY 2 cut(s) 235, 570
AfaI GTAC 1 cut(s) 1074
AfiI CCNNNNNNNGG 4 cut(s) 52, 53, 681, 782
AflIII ACRYGT 1 cut(s) 748
AgsI TTSAA 3 cut(s) 212, 809, 1114
AjiI CACGTC 2 cut(s) 751, 1052
AjnI CCWGG 2 cut(s) 162, 731
AloI GAACNNNNNNTCC 4 cut(s) 614, 646, 819, 851
AluBI AGCT 6 cut(s) 17, 131, 171, 410, 463, 692
AluI AGCT 6 cut(s) 17, 131, 171, 410, 463, 692
Alw21I GWGCWC 2 cut(s) 343, 694
Alw26I GTCTC 1 cut(s) 1132
AlwI GGATC 1 cut(s) 778
Ama87I CYCGRG 1 cut(s) 839
AoxI GGCC 3 cut(s) 856, 884, 922
ApeKI GCWGC 4 cut(s) 131, 392, 407, 947
ApoI RAATTY 2 cut(s) 235, 570
ArsI GACNNNNNNTTYG 4 cut(s) 347, 379, 852, 884
AseI ATTAAT 1 cut(s) 285
AspLEI GCGC 1 cut(s) 88
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 1 cut(s) 383
AvaI CYCGRG 1 cut(s) 839
BaeI ACNNNNGTAYC 2 cut(s) 747, 780
BalI TGGCCA 1 cut(s) 924
BanII GRGCYC 1 cut(s) 694
Bbv12I GWGCWC 2 cut(s) 343, 694
BbvI GCAGC 4 cut(s) 118, 404, 419, 934
BccI CCATC 3 cut(s) 46, 51, 209
BcgI CGANNNNNNTGC 2 cut(s) 1010, 1044
BciT130I CCWGG 2 cut(s) 164, 733
BciVI GTATCC 1 cut(s) 1122
BclI TGATCA 2 cut(s) 442, 891
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 1 cut(s) 1132
BfaI CTAG 2 cut(s) 83, 306
BfmI CTRYAG 1 cut(s) 759
BfuI GTATCC 1 cut(s) 1122
BglII AGATCT 1 cut(s) 1057
BisI GCNGC 5 cut(s) 132, 393, 408, 675, 948
BlsI GCNGC 5 cut(s) 133, 394, 409, 676, 949
Bme1390I CCNGG 3 cut(s) 164, 620, 733
BmeT110I CYCGRG 1 cut(s) 839
BmgBI CACGTC 2 cut(s) 751, 1052
BmrFI CCNGG 3 cut(s) 164, 620, 733
BmsI GCATC 2 cut(s) 661, 934
BpmI CTGGAG 2 cut(s) 171, 708
BpuMI CCSGG 1 cut(s) 620
BsaI GGTCTC 1 cut(s) 1132
BsaJI CCNNGG 1 cut(s) 731
BsaXI ACNNNNNCTCC 2 cut(s) 819, 849
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 53, 681, 782
Bse1I ACTGG 2 cut(s) 925, 1051
Bse3DI GCAATG 1 cut(s) 387
BseBI CCWGG 2 cut(s) 164, 733
BseDI CCNNGG 1 cut(s) 731
BseGI GGATG 3 cut(s) 62, 196, 305
BseLI CCNNNNNNNGG 4 cut(s) 52, 53, 681, 782
BseMI GCAATG 1 cut(s) 387
BseNI ACTGG 2 cut(s) 925, 1051
BseRI GAGGAG 1 cut(s) 1080
BseXI GCAGC 4 cut(s) 118, 404, 419, 934
BsgI GTGCAG 1 cut(s) 946
Bsh1236I CGCG 2 cut(s) 88, 526
BshFI GGCC 3 cut(s) 858, 886, 924
BsiHKAI GWGCWC 2 cut(s) 343, 694
BsiHKCI CYCGRG 1 cut(s) 839
BsiSI CCGG 1 cut(s) 620
BslFI GGGAC 1 cut(s) 759
BslI CCNNNNNNNGG 4 cut(s) 52, 53, 681, 782
BsmAI GTCTC 1 cut(s) 1132
BsmFI GGGAC 1 cut(s) 759
BsmI GAATGC 1 cut(s) 179
BsnI GGCC 3 cut(s) 858, 886, 924
Bso31I GGTCTC 1 cut(s) 1132
BsoBI CYCGRG 1 cut(s) 839
Bsp1286I GDGCHC 2 cut(s) 343, 694
Bsp1407I TGTACA 1 cut(s) 1072
Bsp143I GATC 4 cut(s) 442, 770, 891, 1057
BspACI CCGC 3 cut(s) 526, 575, 675
BspANI GGCC 3 cut(s) 858, 886, 924
BspFNI CGCG 2 cut(s) 88, 526
BspHI TCATGA 1 cut(s) 445
BspPI GGATC 1 cut(s) 778
BspQI GCTCTTC 1 cut(s) 348
BspTNI GGTCTC 1 cut(s) 1132
BsrDI GCAATG 1 cut(s) 387
BsrGI TGTACA 1 cut(s) 1072
BsrI ACTGG 2 cut(s) 925, 1051
BssECI CCNNGG 1 cut(s) 731
BssMI GATC 4 cut(s) 442, 770, 891, 1057
BssNAI GTATAC 1 cut(s) 721
Bst1107I GTATAC 1 cut(s) 721
Bst2UI CCWGG 2 cut(s) 164, 733
Bst4CI ACNGT 6 cut(s) 7, 296, 418, 540, 566, 760
Bst6I CTCTTC 1 cut(s) 348
BstAUI TGTACA 1 cut(s) 1072
BstC8I GCNNGC 1 cut(s) 714
BstDEI CTNAG 3 cut(s) 504, 548, 982
BstF5I GGATG 3 cut(s) 62, 196, 305
BstFNI CGCG 2 cut(s) 88, 526
BstHHI GCGC 1 cut(s) 88
BstKTI GATC 4 cut(s) 445, 773, 894, 1060
BstMAI GTCTC 1 cut(s) 1132
BstMBI GATC 4 cut(s) 442, 770, 891, 1057
BstMWI GCNNNNNNNGC 1 cut(s) 674
BstNI CCWGG 2 cut(s) 164, 733
BstSCI CCNGG 3 cut(s) 162, 618, 731
BstSFI CTRYAG 1 cut(s) 759
BstUI CGCG 2 cut(s) 88, 526
BstV1I GCAGC 4 cut(s) 118, 404, 419, 934
BstX2I RGATCY 2 cut(s) 770, 1057
BstYI RGATCY 2 cut(s) 770, 1057
BstZ17I GTATAC 1 cut(s) 721
BsuI GTATCC 1 cut(s) 1122
BsuRI GGCC 3 cut(s) 858, 886, 924
BtrI CACGTC 2 cut(s) 751, 1052
BtsCI GGATG 3 cut(s) 62, 196, 305
BtsI GCAGTG 2 cut(s) 787, 958
BtsIMutI CAGTG 5 cut(s) 117, 414, 787, 932, 958
Cac8I GCNNGC 1 cut(s) 714
CciI TCATGA 1 cut(s) 445
CfoI GCGC 1 cut(s) 88
CseI GACGC 1 cut(s) 77
Csp6I GTAC 1 cut(s) 1073
CviAII CATG 3 cut(s) 108, 446, 695
CviQI GTAC 1 cut(s) 1073
DdeI CTNAG 3 cut(s) 504, 548, 982
DpnI GATC 4 cut(s) 444, 772, 893, 1059
DpnII GATC 4 cut(s) 442, 770, 891, 1057
EaeI YGGCCR 1 cut(s) 922
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
EciI GGCGGA 1 cut(s) 564
Ecl136II GAGCTC 1 cut(s) 692
Eco24I GRGCYC 1 cut(s) 694
Eco31I GGTCTC 1 cut(s) 1132
Eco53kI GAGCTC 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 839
EcoICRI GAGCTC 1 cut(s) 692
EcoRI GAATTC 1 cut(s) 570
EcoRII CCWGG 2 cut(s) 162, 731
EcoT38I GRGCYC 1 cut(s) 694
FaeI CATG 3 cut(s) 111, 449, 698
FaqI GGGAC 1 cut(s) 759
FatI CATG 3 cut(s) 107, 445, 694
FbaI TGATCA 2 cut(s) 442, 891
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 5 cut(s) 132, 393, 408, 675, 948
FokI GGATG 3 cut(s) 69, 183, 312
FriOI GRGCYC 1 cut(s) 694
Fsp4HI GCNGC 5 cut(s) 132, 393, 408, 675, 948
FspBI CTAG 2 cut(s) 83, 306
GlaI GCGC 1 cut(s) 87
GluI GCNGC 5 cut(s) 132, 393, 408, 675, 948
GsuI CTGGAG 2 cut(s) 171, 708
HaeIII GGCC 3 cut(s) 858, 886, 924
HapII CCGG 1 cut(s) 620
HgaI GACGC 1 cut(s) 77
HhaI GCGC 1 cut(s) 88
Hin1II CATG 3 cut(s) 111, 449, 698
Hin6I GCGC 1 cut(s) 86
HinP1I GCGC 1 cut(s) 86
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 6 cut(s) 449, 830, 911, 1022, 1106, 1147
HpaII CCGG 1 cut(s) 620
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 5 cut(s) 26, 323, 633, 721, 766
Hpy188I TCNGA 3 cut(s) 13, 559, 910
Hpy8I GTNNAC 5 cut(s) 26, 323, 633, 721, 766
Hpy99I CGWCG 2 cut(s) 570, 755
HpyAV CCTTC 1 cut(s) 523
HpyCH4III ACNGT 6 cut(s) 7, 296, 418, 540, 566, 760
HpyCH4IV ACGT 4 cut(s) 750, 934, 1051, 1078
HpyCH4V TGCA 5 cut(s) 392, 407, 958, 963, 1067
HpyF10VI GCNNNNNNNGC 1 cut(s) 674
HpyF3I CTNAG 3 cut(s) 504, 548, 982
HpySE526I ACGT 4 cut(s) 750, 934, 1051, 1078
Hsp92II CATG 3 cut(s) 111, 449, 698
HspAI GCGC 1 cut(s) 86
Ksp22I TGATCA 2 cut(s) 442, 891
Kzo9I GATC 4 cut(s) 442, 770, 891, 1057
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 1 cut(s) 689
Lsp1109I GCAGC 4 cut(s) 118, 404, 419, 934
LweI GCATC 2 cut(s) 661, 934
MaeI CTAG 2 cut(s) 83, 306
MaeII ACGT 4 cut(s) 750, 934, 1051, 1078
MaeIII GTNAC 2 cut(s) 412, 993
MalI GATC 4 cut(s) 444, 772, 893, 1059
MboI GATC 4 cut(s) 442, 770, 891, 1057
MboII GAAGA 7 cut(s) 167, 200, 234, 335, 480, 860, 1067
MflI RGATCY 2 cut(s) 770, 1057
MhlI GDGCHC 2 cut(s) 343, 694
MlsI TGGCCA 1 cut(s) 924
MluCI AATT 7 cut(s) 213, 235, 282, 286, 570, 786, 895
MluNI TGGCCA 1 cut(s) 924
MlyI GAGTC 2 cut(s) 839, 920
Mox20I TGGCCA 1 cut(s) 924
MscI TGGCCA 1 cut(s) 924
MseI TTAA 5 cut(s) 285, 291, 581, 626, 680
MslI CAYNNNNRTG 1 cut(s) 122
Msp20I TGGCCA 1 cut(s) 924
MspA1I CMGCKG 1 cut(s) 410
MspI CCGG 1 cut(s) 620
MspR9I CCNGG 3 cut(s) 164, 620, 733
Mva1269I GAATGC 1 cut(s) 179
MvaI CCWGG 2 cut(s) 164, 733
MvnI CGCG 2 cut(s) 88, 526
MwoI GCNNNNNNNGC 1 cut(s) 674
NciI CCSGG 1 cut(s) 620
NdeII GATC 4 cut(s) 442, 770, 891, 1057
NlaIII CATG 3 cut(s) 111, 449, 698
NmuCI GTSAC 1 cut(s) 412
PagI TCATGA 1 cut(s) 445
PciSI GCTCTTC 1 cut(s) 348
PctI GAATGC 1 cut(s) 179
PfeI GAWTC 4 cut(s) 449, 1022, 1106, 1147
PflFI GACNNNGTC 1 cut(s) 1013
PflMI CCANNNNNTGG 1 cut(s) 782
PfoI TCCNGGA 1 cut(s) 618
PkrI GCNGC 5 cut(s) 133, 394, 409, 676, 949
PleI GAGTC 2 cut(s) 838, 919
PpsI GAGTC 2 cut(s) 838, 919
PshBI ATTAAT 1 cut(s) 285
PsiI TTATAA 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 694
Psp1406I AACGTT 1 cut(s) 1078
Psp6I CCWGG 2 cut(s) 162, 731
PspGI CCWGG 2 cut(s) 162, 731
PsuI RGATCY 2 cut(s) 770, 1057
PsyI GACNNNGTC 1 cut(s) 1013
PvuII CAGCTG 1 cut(s) 410
RsaI GTAC 1 cut(s) 1074
RsaNI GTAC 1 cut(s) 1073
RseI CAYNNNNRTG 1 cut(s) 122
SacI GAGCTC 1 cut(s) 694
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 5 cut(s) 285, 291, 581, 626, 680
SatI GCNGC 5 cut(s) 132, 393, 408, 675, 948
Sau3AI GATC 4 cut(s) 442, 770, 891, 1057
SchI GAGTC 2 cut(s) 839, 920
ScrFI CCNGG 3 cut(s) 164, 620, 733
SduI GDGCHC 2 cut(s) 343, 694
SfaNI GCATC 2 cut(s) 661, 934
SfcI CTRYAG 1 cut(s) 759
SmiMI CAYNNNNRTG 1 cut(s) 122
Sse9I AATT 7 cut(s) 213, 235, 282, 286, 570, 786, 895
SsiI CCGC 3 cut(s) 526, 575, 675
SspMI CTAG 2 cut(s) 83, 306
SstI GAGCTC 1 cut(s) 694
StyD4I CCNGG 3 cut(s) 162, 618, 731
TaaI ACNGT 6 cut(s) 7, 296, 418, 540, 566, 760
TaiI ACGT 4 cut(s) 753, 937, 1054, 1081
TaqI TCGA 5 cut(s) 427, 568, 753, 871, 1020
TasI AATT 7 cut(s) 213, 235, 282, 286, 570, 786, 895
TatI WGTACW 1 cut(s) 1072
TauI GCSGC 1 cut(s) 677
TfiI GAWTC 4 cut(s) 449, 1022, 1106, 1147
Tru1I TTAA 5 cut(s) 285, 291, 581, 626, 680
Tru9I TTAA 5 cut(s) 285, 291, 581, 626, 680
TscAI CASTG 5 cut(s) 124, 421, 787, 932, 965
TseFI GTSAC 1 cut(s) 412
TseI GCWGC 4 cut(s) 131, 392, 407, 947
Tsp45I GTSAC 1 cut(s) 412
TspDTI ATGAA 7 cut(s) 294, 441, 463, 467, 480, 502, 1014
TspGWI ACGGA 1 cut(s) 783
TspRI CASTG 5 cut(s) 124, 421, 787, 932, 965
Tth111I GACNNNGTC 1 cut(s) 1013
Van91I CCANNNNNTGG 1 cut(s) 782
VspI ATTAAT 1 cut(s) 285
XapI RAATTY 2 cut(s) 235, 570
XcmI CCANNNNNNNNNTGG 1 cut(s) 911
XmiI GTMKAC 1 cut(s) 720
XspI CTAG 2 cut(s) 83, 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.