Rh1AG021300

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0008425
Physical Location & Seq
Reverse (-)
3921279 .. 3926825
5547 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG021300.1

Sequence Viewer

Length: 1287 bp
ATGAAGCTTGCAACTTGTTCATATTCGTCGTCACCAACCTCCGTTACTCTTGCCCGTCCCAGATTCTCATTCAATATATTTCAGCTCTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAACCGCCGCCCGCCAACCACGACCCGCAATGGTTTCCGCTCGCAACTACGCCCACGCGCACTACCTTCCTCCTTGGTTCAGTGTGGCTCCTATGATGGAATGGACTGATGTTCACTACAGGACTATGGCCCGCCTCATATCCAAGCACGCCTGGCTCTACACTGAGATGCTCGCTGCCGAAACCATTGTCTATCAGAAGGACAATCTGGACAGATTCTTGTACTTTCCTCCAGAACAGCATCCTATTGTTCTACAAATCGGTGGCAGTAACTTAGAAAACCTGGCAAAAGCTACTCAACTTGCTAATATTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCCAGTCCAAGAGTAGCTGGACATGGGTGCTTCGGTGCTCGTCTTATGCTGGATCCAAAGTTTGTTGCCGAGGCTATGTCAGTGATTGCTGCAAACACAGATGTCCCTGTCAGCGTTAAGTGTCGAATTGGTGTTGATGATCACGATTCATATAATGAGCTCTGTGATTTTATTTATAAAGTTTCTTCTCAATCACCAACTAGGCACTTCATTATACATTCTCGGAAGGCCATGCTGAATGGAATCAGCCCAGCTGAAAATCGAAGTATTCCACCTCTTAAATACGAGTACTTTTATGGCCTCTTGCGTGACTTTCCGGACTTAAGATTTACCATAAATGGTGGAATAAACACTATTGATGAGGTCAATGCAGCTAGGAAAGCAGGAGCTCATGGTGTAATGGTTGGACGGGCTGCATTTAATAACTCATGGAATACTTTGGGACATGTTGATACTGCTATTTATGGTGCACCAAGCAGTGGTGTAACACGGCGTCAGTTACTCAAGGAGTTTGAAGCTTATGGAGAGACTGTTTTGGGAAAATATGGACAAAAGCCCAATGTTCGAGATGTGGCCAAGCCTTTGCTTAGCCTTTTCTATGCTCAGCCTGGGAACAGCCTTTGGAAACGCAAAGCTGATGCTGCTTTCATGCATTGCACGACTATGAAATCATTTTTTGAGGAAACACTTGTAGCAATTCCAGACTTTGTCTTGGATGCACCTGTTGGAGCGCCACCATCTGGTAGTAATGATCCTTTTTCAAACATACATACTTTGTTGCCTCCACCATACGAATCAAGGGAACAAGAGCTACAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

428

Amino Acids

47.62

Weight (kDa)

8.35

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 67 - 374 2.2e-57 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 639
AccB7I CCANNNNNTGG 2 cut(s) 941, 1202
AccBSI CCGCTC 1 cut(s) 155
AccII CGCG 1 cut(s) 173
AccIII TCCGGA 1 cut(s) 778
AciI CCGC 6 cut(s) 120, 123, 127, 141, 153, 247
AclWI GGATC 3 cut(s) 509, 522, 1208
AcoI YGGCCR 1 cut(s) 1035
AcyI GRCGYC 1 cut(s) 955
AfaI GTAC 2 cut(s) 338, 752
AfiI CCNNNNNNNGG 2 cut(s) 941, 1202
AflII CTTAAG 1 cut(s) 784
AflIII ACRYGT 1 cut(s) 907
AgsI TTSAA 4 cut(s) 73, 117, 977, 1224
AjnI CCWGG 3 cut(s) 266, 396, 1069
Alw21I GWGCWC 4 cut(s) 502, 624, 853, 934
Alw26I GTCTC 1 cut(s) 983
Alw44I GTGCAC 1 cut(s) 930
AlwI GGATC 3 cut(s) 509, 522, 1208
Aor13HI TCCGGA 1 cut(s) 778
AoxI GGCC 4 cut(s) 243, 690, 760, 1035
ApaLI GTGCAC 1 cut(s) 930
ApeKI GCWGC 5 cut(s) 290, 551, 833, 875, 1103
ArsI GACNNNNNNTTYG 2 cut(s) 288, 320
AspLEI GCGC 2 cut(s) 175, 1195
AspS9I GGNCC 1 cut(s) 244
AsuHPI GGTGA 2 cut(s) 24, 648
BaeGI GKGCMC 1 cut(s) 934
BalI TGGCCA 1 cut(s) 1037
BamHI GGATCC 1 cut(s) 514
BanII GRGCYC 2 cut(s) 624, 853
Bbv12I GWGCWC 4 cut(s) 502, 624, 853, 934
BbvI GCAGC 5 cut(s) 277, 538, 845, 862, 1090
BccI CCATC 2 cut(s) 205, 1207
BceAI ACGGC 1 cut(s) 968
BciT130I CCWGG 3 cut(s) 268, 398, 1071
BclI TGATCA 1 cut(s) 601
BcoDI GTCTC 1 cut(s) 983
BfaI CTAG 2 cut(s) 663, 837
BfmI CTRYAG 1 cut(s) 232
BfoI RGCGCY 1 cut(s) 1196
BfrI CTTAAG 1 cut(s) 784
BisI GCNGC 6 cut(s) 123, 291, 552, 834, 876, 1104
BlpI GCTNAGC 2 cut(s) 1049, 1065
BlsI GCNGC 6 cut(s) 124, 292, 553, 835, 877, 1105
BmcAI AGTACT 1 cut(s) 752
Bme1390I CCNGG 3 cut(s) 268, 398, 1071
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 2 cut(s) 204, 516
BmrFI CCNGG 3 cut(s) 268, 398, 1071
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 4 cut(s) 273, 364, 1090, 1168
BmuI ACTGGG 1 cut(s) 459
BpmI CTGGAG 1 cut(s) 330
Bpu1102I GCTNAGC 2 cut(s) 1049, 1065
BpuEI CTTGAG 1 cut(s) 950
BsaHI GRCGYC 1 cut(s) 955
BsaJI CCNNGG 3 cut(s) 188, 531, 1070
BsaWI WCCGGW 1 cut(s) 778
BsaXI ACNNNNNCTCC 2 cut(s) 978, 1008
Bsc4I CCNNNNNNNGG 2 cut(s) 941, 1202
Bse1I ACTGG 1 cut(s) 465
Bse3DI GCAATG 2 cut(s) 150, 1114
BseAI TCCGGA 1 cut(s) 778
BseBI CCWGG 3 cut(s) 268, 398, 1071
BseDI CCNNGG 3 cut(s) 188, 531, 1070
BseGI GGATG 3 cut(s) 355, 464, 1183
BseLI CCNNNNNNNGG 2 cut(s) 941, 1202
BseMI GCAATG 2 cut(s) 150, 1114
BseMII CTCAG 2 cut(s) 270, 1079
BseNI ACTGG 1 cut(s) 465
BseSI GKGCMC 1 cut(s) 934
BseXI GCAGC 5 cut(s) 277, 538, 845, 862, 1090
BseYI CCCAGC 1 cut(s) 712
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 4 cut(s) 245, 692, 762, 1037
BsiHKAI GWGCWC 4 cut(s) 502, 624, 853, 934
BsiSI CCGG 1 cut(s) 779
BslFI GGGAC 4 cut(s) 42, 447, 551, 918
BslI CCNNNNNNNGG 2 cut(s) 941, 1202
BsmAI GTCTC 1 cut(s) 983
BsmFI GGGAC 4 cut(s) 42, 447, 551, 918
BsnI GGCC 4 cut(s) 245, 692, 762, 1037
Bsp1286I GDGCHC 4 cut(s) 502, 624, 853, 934
Bsp13I TCCGGA 1 cut(s) 778
Bsp143I GATC 4 cut(s) 441, 514, 601, 1213
Bsp1720I GCTNAGC 2 cut(s) 1049, 1065
BspACI CCGC 6 cut(s) 120, 123, 127, 141, 153, 247
BspANI GGCC 4 cut(s) 245, 692, 762, 1037
BspCNI CTCAG 2 cut(s) 271, 1078
BspEI TCCGGA 1 cut(s) 778
BspFNI CGCG 1 cut(s) 173
BspLI GGNNCC 2 cut(s) 204, 516
BspPI GGATC 3 cut(s) 509, 522, 1208
BspTI CTTAAG 1 cut(s) 784
BsrBI CCGCTC 1 cut(s) 155
BsrDI GCAATG 2 cut(s) 150, 1114
BsrI ACTGG 1 cut(s) 465
BssECI CCNNGG 3 cut(s) 188, 531, 1070
BssMI GATC 4 cut(s) 441, 514, 601, 1213
BssNI GRCGYC 1 cut(s) 955
BssT1I CCWWGG 1 cut(s) 188
Bst2UI CCWGG 3 cut(s) 268, 398, 1071
Bst4CI ACNGT 1 cut(s) 994
Bst6I CTCTTC 1 cut(s) 94
BstACI GRCGYC 1 cut(s) 955
BstAFI CTTAAG 1 cut(s) 784
BstC8I GCNNGC 6 cut(s) 9, 127, 157, 247, 264, 288
BstDEI CTNAG 5 cut(s) 279, 388, 1049, 1065, 1284
BstF5I GGATG 3 cut(s) 355, 464, 1183
BstFNI CGCG 1 cut(s) 173
BstH2I RGCGCY 1 cut(s) 1196
BstHHI GCGC 2 cut(s) 175, 1195
BstKTI GATC 4 cut(s) 444, 517, 604, 1216
BstMAI GTCTC 1 cut(s) 983
BstMBI GATC 4 cut(s) 441, 514, 601, 1213
BstMWI GCNNNNNNNGC 3 cut(s) 268, 842, 1103
BstNI CCWGG 3 cut(s) 268, 398, 1071
BstNSI RCATGY 1 cut(s) 911
BstSCI CCNGG 3 cut(s) 266, 396, 1069
BstSFI CTRYAG 1 cut(s) 232
BstSLI GKGCMC 1 cut(s) 934
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 5 cut(s) 277, 538, 845, 862, 1090
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 4 cut(s) 245, 692, 762, 1037
BtsCI GGATG 3 cut(s) 355, 464, 1183
BtsI GCAGTG 1 cut(s) 946
BtsIMutI CAGTG 4 cut(s) 202, 276, 549, 946
Cac8I GCNNGC 6 cut(s) 9, 127, 157, 247, 264, 288
CfoI GCGC 2 cut(s) 175, 1195
Cfr13I GGNCC 1 cut(s) 244
CseI GACGC 1 cut(s) 944
Csp6I GTAC 2 cut(s) 337, 751
CviAII CATG 6 cut(s) 485, 694, 854, 891, 908, 1111
CviQI GTAC 2 cut(s) 337, 751
DdeI CTNAG 5 cut(s) 279, 388, 1049, 1065, 1284
DpnI GATC 4 cut(s) 443, 516, 603, 1215
DpnII GATC 4 cut(s) 441, 514, 601, 1213
EaeI YGGCCR 1 cut(s) 1035
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
Ecl136II GAGCTC 2 cut(s) 622, 851
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 2 cut(s) 624, 853
Eco53kI GAGCTC 2 cut(s) 622, 851
EcoICRI GAGCTC 2 cut(s) 622, 851
EcoRII CCWGG 3 cut(s) 266, 396, 1069
EcoT14I CCWWGG 1 cut(s) 188
EcoT22I ATGCAT 1 cut(s) 1116
EcoT38I GRGCYC 2 cut(s) 624, 853
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 6 cut(s) 488, 697, 857, 894, 911, 1114
FaqI GGGAC 4 cut(s) 42, 447, 551, 918
FatI CATG 6 cut(s) 484, 693, 853, 890, 907, 1110
FauI CCCGC 3 cut(s) 134, 148, 254
FbaI TGATCA 1 cut(s) 601
Fnu4HI GCNGC 6 cut(s) 123, 291, 552, 834, 876, 1104
FokI GGATG 3 cut(s) 342, 471, 1190
FriOI GRGCYC 2 cut(s) 624, 853
Fsp4HI GCNGC 6 cut(s) 123, 291, 552, 834, 876, 1104
FspBI CTAG 2 cut(s) 663, 837
GlaI GCGC 2 cut(s) 174, 1194
GluI GCNGC 6 cut(s) 123, 291, 552, 834, 876, 1104
GsaI CCCAGC 1 cut(s) 716
GsuI CTGGAG 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 1196
HaeIII GGCC 4 cut(s) 245, 692, 762, 1037
HapII CCGG 1 cut(s) 779
HgaI GACGC 1 cut(s) 944
HhaI GCGC 2 cut(s) 175, 1195
Hin1I GRCGYC 1 cut(s) 955
Hin1II CATG 6 cut(s) 488, 697, 857, 894, 911, 1114
Hin6I GCGC 2 cut(s) 173, 1193
HinP1I GCGC 2 cut(s) 173, 1193
HindIII AAGCTT 2 cut(s) 5, 978
HinfI GANTC 5 cut(s) 63, 330, 608, 705, 1256
HpaII CCGG 1 cut(s) 779
HphI GGTGA 2 cut(s) 24, 648
Hpy166II GTNNAC 2 cut(s) 229, 932
Hpy188I TCNGA 2 cut(s) 312, 687
Hpy188III TCNNGA 6 cut(s) 323, 347, 605, 779, 1028, 1163
Hpy8I GTNNAC 2 cut(s) 229, 932
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 3 cut(s) 191, 307, 682
HpyCH4III ACNGT 1 cut(s) 994
HpyCH4V TGCA 8 cut(s) 11, 554, 833, 878, 932, 1114, 1119, 1181
HpyF10VI GCNNNNNNNGC 3 cut(s) 268, 842, 1103
HpyF3I CTNAG 5 cut(s) 279, 388, 1049, 1065, 1284
Hsp92I GRCGYC 1 cut(s) 955
Hsp92II CATG 6 cut(s) 488, 697, 857, 894, 911, 1114
HspAI GCGC 2 cut(s) 173, 1193
Kpn2I TCCGGA 1 cut(s) 778
Ksp22I TGATCA 1 cut(s) 601
Kzo9I GATC 4 cut(s) 441, 514, 601, 1213
LmnI GCTCC 3 cut(s) 208, 848, 1190
Lsp1109I GCAGC 5 cut(s) 277, 538, 845, 862, 1090
LweI GCATC 4 cut(s) 273, 364, 1090, 1168
MaeI CTAG 2 cut(s) 663, 837
MaeIII GTNAC 6 cut(s) 30, 43, 383, 770, 946, 960
MalI GATC 4 cut(s) 443, 516, 603, 1215
MbiI CCGCTC 1 cut(s) 155
MboI GATC 4 cut(s) 441, 514, 601, 1213
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 4 cut(s) 502, 624, 853, 934
MlsI TGGCCA 1 cut(s) 1037
MluCI AATT 4 cut(s) 445, 451, 588, 1158
MluNI TGGCCA 1 cut(s) 1037
MmeI TCCRAC 2 cut(s) 847, 1168
Mox20I TGGCCA 1 cut(s) 1037
Mph1103I ATGCAT 1 cut(s) 1116
MroI TCCGGA 1 cut(s) 778
MscI TGGCCA 1 cut(s) 1037
MseI TTAA 5 cut(s) 450, 579, 741, 785, 882
MslI CAYNNNNRTG 2 cut(s) 281, 1124
Msp20I TGGCCA 1 cut(s) 1037
MspA1I CMGCKG 1 cut(s) 716
MspCI CTTAAG 1 cut(s) 784
MspI CCGG 1 cut(s) 779
MspR9I CCNGG 3 cut(s) 268, 398, 1071
MvaI CCWGG 3 cut(s) 268, 398, 1071
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 3 cut(s) 268, 842, 1103
NdeII GATC 4 cut(s) 441, 514, 601, 1213
NlaIII CATG 6 cut(s) 488, 697, 857, 894, 911, 1114
NlaIV GGNNCC 2 cut(s) 204, 516
NmeAIII GCCGAG 1 cut(s) 556
NmuCI GTSAC 2 cut(s) 30, 770
NsiI ATGCAT 1 cut(s) 1116
NspI RCATGY 1 cut(s) 911
PciI ACATGT 1 cut(s) 907
PfeI GAWTC 5 cut(s) 63, 330, 608, 705, 1256
PflFI GACNNNGTC 1 cut(s) 1169
PflMI CCANNNNNTGG 2 cut(s) 941, 1202
PkrI GCNGC 6 cut(s) 124, 292, 553, 835, 877, 1105
PscI ACATGT 1 cut(s) 907
PsiI TTATAA 1 cut(s) 639
Psp124BI GAGCTC 2 cut(s) 624, 853
Psp6I CCWGG 3 cut(s) 266, 396, 1069
PspFI CCCAGC 1 cut(s) 712
PspGI CCWGG 3 cut(s) 266, 396, 1069
PspN4I GGNNCC 2 cut(s) 204, 516
PspPI GGNCC 1 cut(s) 244
PsrI GAACNNNNNNTAC 1 cut(s) 1257
PsuI RGATCY 1 cut(s) 514
PsyI GACNNNGTC 1 cut(s) 1169
PvuII CAGCTG 1 cut(s) 716
RsaI GTAC 2 cut(s) 338, 752
RsaNI GTAC 2 cut(s) 337, 751
RseI CAYNNNNRTG 2 cut(s) 281, 1124
SacI GAGCTC 2 cut(s) 624, 853
SaqAI TTAA 5 cut(s) 450, 579, 741, 785, 882
SatI GCNGC 6 cut(s) 123, 291, 552, 834, 876, 1104
Sau3AI GATC 4 cut(s) 441, 514, 601, 1213
Sau96I GGNCC 1 cut(s) 244
ScaI AGTACT 1 cut(s) 752
ScrFI CCNGG 3 cut(s) 268, 398, 1071
SduI GDGCHC 4 cut(s) 502, 624, 853, 934
SfaNI GCATC 4 cut(s) 273, 364, 1090, 1168
SfcI CTRYAG 1 cut(s) 232
SmiMI CAYNNNNRTG 2 cut(s) 281, 1124
SmlI CTYRAG 2 cut(s) 784, 965
SmoI CTYRAG 2 cut(s) 784, 965
Sse9I AATT 4 cut(s) 445, 451, 588, 1158
SsiI CCGC 6 cut(s) 120, 123, 127, 141, 153, 247
SspI AATATT 1 cut(s) 424
SspMI CTAG 2 cut(s) 663, 837
SstI GAGCTC 2 cut(s) 624, 853
StyD4I CCNGG 3 cut(s) 266, 396, 1069
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 1 cut(s) 994
TaqI TCGA 3 cut(s) 586, 724, 1027
TasI AATT 4 cut(s) 445, 451, 588, 1158
TatI WGTACW 2 cut(s) 336, 750
TauI GCSGC 1 cut(s) 125
TfiI GAWTC 5 cut(s) 63, 330, 608, 705, 1256
Tru1I TTAA 5 cut(s) 450, 579, 741, 785, 882
Tru9I TTAA 5 cut(s) 450, 579, 741, 785, 882
TscAI CASTG 4 cut(s) 202, 283, 549, 946
TseFI GTSAC 2 cut(s) 30, 770
TseI GCWGC 5 cut(s) 290, 551, 833, 875, 1103
Tsp45I GTSAC 2 cut(s) 30, 770
TspDTI ATGAA 6 cut(s) 9, 17, 600, 661, 1099, 1142
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 4 cut(s) 202, 283, 549, 946
Tth111I GACNNNGTC 1 cut(s) 1169
Van91I CCANNNNNTGG 2 cut(s) 941, 1202
Vha464I CTTAAG 1 cut(s) 784
VneI GTGCAC 1 cut(s) 930
XceI RCATGY 1 cut(s) 911
XspI CTAG 2 cut(s) 663, 837
ZrmI AGTACT 1 cut(s) 752
Zsp2I ATGCAT 1 cut(s) 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.