Rh1CG019300

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0008425
Physical Location & Seq
Reverse (-)
3228217 .. 3233460
5244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG019300.1

Sequence Viewer

Length: 912 bp
ATGGTTTCCGCTCGCAACTACGCCCACGCCCGCTACCTTCCTCCTTGGTTCAGTGTGGCTCCTATGATGGAATGGACTGATGTTCACTACAGGACTATGGCCCGCCTAATTTCCAAGCATGCCTGGCTCTACACTGAGATGCTCGCTGCCGAAACCATTGTCTATCAGAAGGACAATCTGGACAGATTCTTGTACTTTCCTCCAGAACAGCATCCTATTGTTCTACAAATTGGTGGCAGTAACTTAGAAAACCTGGCAAAAGCTACTCAACTTGCTAATATTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCCAGTCCAAGAGTAGCTGGACATGGGTGCTTCGGTGCTCGTCTTATGCTGGATCCAAAGTTTGTTGCCGAGGCTATGTCAGTGATTGCTGCGAACACAGATGTCCCTGTCAGCGTTAAGTGTCGAATTGGTGTTGATGATCACGATTCATATAATGAGCTCTGTGATTTTATTTATAAAGTTTCTTCTCAATCACCAACTAGGCACTTCATTATACATTCTCGGAAGGCCATGCTGAATGGAATCAGCCCAGCTGAAAATCGAAGTATTCCACCTCTTAAATACGAGTACTTTTATGGCCTCTTGCGTGACTTTCCGGACTTAAGATTTACCATAAATGGTGGAATAAACACTATTGATGAGGTCAATGCAGCTAGGAAAGCAGGAGCTCATGGTGTAATGGTTGGACGGGCTGCATTTAATAACTCGTGGAATACTTTGGGACATGTTGATACTGCTATTTATGGTGCACCAAGCAGCGGTATAACACGGCGTCAGTTACTCAAGGAGTTTGAAGCTTATGGAGAGACTGTTTTGGGAAAATATGGACAAAAGCCCAATGTTCGAGATGTGGCCAAGGTTAGTACTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

303

Amino Acids

33.98

Weight (kDa)

8.48

Isoelectric Point (pI)

31.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 19 - 299 1e-53 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
AccBSI CCGCTC 1 cut(s) 11
AccIII TCCGGA 1 cut(s) 634
AciI CCGC 4 cut(s) 9, 31, 103, 798
AclWI GGATC 2 cut(s) 365, 378
AcoI YGGCCR 1 cut(s) 891
AcyI GRCGYC 1 cut(s) 811
AfaI GTAC 3 cut(s) 194, 608, 904
AfiI CCNNNNNNNGG 1 cut(s) 797
AflII CTTAAG 1 cut(s) 640
AflIII ACRYGT 1 cut(s) 763
AgsI TTSAA 1 cut(s) 833
AjnI CCWGG 2 cut(s) 122, 252
AluBI AGCT 8 cut(s) 263, 335, 478, 572, 692, 707, 836, 909
AluI AGCT 8 cut(s) 263, 335, 478, 572, 692, 707, 836, 909
Alw21I GWGCWC 4 cut(s) 358, 480, 709, 790
Alw26I GTCTC 1 cut(s) 839
Alw44I GTGCAC 1 cut(s) 786
AlwI GGATC 2 cut(s) 365, 378
Aor13HI TCCGGA 1 cut(s) 634
AoxI GGCC 4 cut(s) 99, 546, 616, 891
ApaLI GTGCAC 1 cut(s) 786
ApeKI GCWGC 5 cut(s) 146, 407, 689, 731, 795
ArsI GACNNNNNNTTYG 2 cut(s) 144, 176
AspS9I GGNCC 1 cut(s) 100
AsuHPI GGTGA 1 cut(s) 504
BaeGI GKGCMC 1 cut(s) 790
BalI TGGCCA 1 cut(s) 893
BamHI GGATCC 1 cut(s) 370
BanII GRGCYC 2 cut(s) 480, 709
BauI CACGAG 1 cut(s) 745
Bbv12I GWGCWC 4 cut(s) 358, 480, 709, 790
BbvI GCAGC 5 cut(s) 133, 394, 701, 718, 807
BccI CCATC 1 cut(s) 61
BceAI ACGGC 1 cut(s) 824
BciT130I CCWGG 2 cut(s) 124, 254
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 839
BfaI CTAG 4 cut(s) 519, 693, 906, 910
BfmI CTRYAG 1 cut(s) 88
BfrI CTTAAG 1 cut(s) 640
BisI GCNGC 5 cut(s) 147, 408, 690, 732, 796
BlsI GCNGC 5 cut(s) 148, 409, 691, 733, 797
BmcAI AGTACT 2 cut(s) 608, 904
Bme1390I CCNGG 2 cut(s) 124, 254
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 2 cut(s) 60, 372
BmrFI CCNGG 2 cut(s) 124, 254
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 2 cut(s) 129, 220
BmuI ACTGGG 1 cut(s) 315
BpmI CTGGAG 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 806
BsaHI GRCGYC 1 cut(s) 811
BsaJI CCNNGG 3 cut(s) 44, 387, 894
BsaWI WCCGGW 1 cut(s) 634
BsaXI ACNNNNNCTCC 2 cut(s) 834, 864
Bsc4I CCNNNNNNNGG 1 cut(s) 797
Bse1I ACTGG 1 cut(s) 321
BseAI TCCGGA 1 cut(s) 634
BseBI CCWGG 2 cut(s) 124, 254
BseDI CCNNGG 3 cut(s) 44, 387, 894
BseGI GGATG 2 cut(s) 211, 320
BseLI CCNNNNNNNGG 1 cut(s) 797
BseMII CTCAG 1 cut(s) 126
BseNI ACTGG 1 cut(s) 321
BseSI GKGCMC 1 cut(s) 790
BseXI GCAGC 5 cut(s) 133, 394, 701, 718, 807
BseYI CCCAGC 1 cut(s) 568
BshFI GGCC 4 cut(s) 101, 548, 618, 893
BsiHKAI GWGCWC 4 cut(s) 358, 480, 709, 790
BsiSI CCGG 1 cut(s) 635
BslFI GGGAC 3 cut(s) 303, 407, 774
BslI CCNNNNNNNGG 1 cut(s) 797
BsmAI GTCTC 1 cut(s) 839
BsmFI GGGAC 3 cut(s) 303, 407, 774
BsnI GGCC 4 cut(s) 101, 548, 618, 893
Bsp1286I GDGCHC 4 cut(s) 358, 480, 709, 790
Bsp13I TCCGGA 1 cut(s) 634
Bsp143I GATC 3 cut(s) 297, 370, 457
BspACI CCGC 4 cut(s) 9, 31, 103, 798
BspANI GGCC 4 cut(s) 101, 548, 618, 893
BspCNI CTCAG 1 cut(s) 127
BspEI TCCGGA 1 cut(s) 634
BspLI GGNNCC 2 cut(s) 60, 372
BspPI GGATC 2 cut(s) 365, 378
BspTI CTTAAG 1 cut(s) 640
BsrBI CCGCTC 1 cut(s) 11
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 3 cut(s) 44, 387, 894
BssMI GATC 3 cut(s) 297, 370, 457
BssNI GRCGYC 1 cut(s) 811
BssSI CACGAG 1 cut(s) 745
BssT1I CCWWGG 2 cut(s) 44, 894
Bst2BI CACGAG 1 cut(s) 745
Bst2UI CCWGG 2 cut(s) 124, 254
Bst4CI ACNGT 1 cut(s) 850
BstACI GRCGYC 1 cut(s) 811
BstAFI CTTAAG 1 cut(s) 640
BstC8I GCNNGC 5 cut(s) 13, 31, 103, 120, 144
BstDEI CTNAG 2 cut(s) 135, 244
BstF5I GGATG 2 cut(s) 211, 320
BstKTI GATC 3 cut(s) 300, 373, 460
BstMAI GTCTC 1 cut(s) 839
BstMBI GATC 3 cut(s) 297, 370, 457
BstMWI GCNNNNNNNGC 2 cut(s) 124, 698
BstNI CCWGG 2 cut(s) 124, 254
BstNSI RCATGY 2 cut(s) 122, 767
BstSCI CCNGG 2 cut(s) 122, 252
BstSFI CTRYAG 1 cut(s) 88
BstSLI GKGCMC 1 cut(s) 790
BstV1I GCAGC 5 cut(s) 133, 394, 701, 718, 807
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 4 cut(s) 101, 548, 618, 893
BtsCI GGATG 2 cut(s) 211, 320
BtsIMutI CAGTG 3 cut(s) 58, 132, 405
Cac8I GCNNGC 5 cut(s) 13, 31, 103, 120, 144
Cfr13I GGNCC 1 cut(s) 100
CseI GACGC 1 cut(s) 800
Csp6I GTAC 3 cut(s) 193, 607, 903
CviAII CATG 5 cut(s) 119, 341, 550, 710, 764
CviQI GTAC 3 cut(s) 193, 607, 903
DdeI CTNAG 2 cut(s) 135, 244
DpnI GATC 3 cut(s) 299, 372, 459
DpnII GATC 3 cut(s) 297, 370, 457
EaeI YGGCCR 1 cut(s) 891
Ecl136II GAGCTC 2 cut(s) 478, 707
Eco130I CCWWGG 2 cut(s) 44, 894
Eco24I GRGCYC 2 cut(s) 480, 709
Eco53kI GAGCTC 2 cut(s) 478, 707
EcoICRI GAGCTC 2 cut(s) 478, 707
EcoRII CCWGG 2 cut(s) 122, 252
EcoT14I CCWWGG 2 cut(s) 44, 894
EcoT38I GRGCYC 2 cut(s) 480, 709
ErhI CCWWGG 2 cut(s) 44, 894
FaeI CATG 5 cut(s) 122, 344, 553, 713, 767
FaqI GGGAC 3 cut(s) 303, 407, 774
FatI CATG 5 cut(s) 118, 340, 549, 709, 763
FauI CCCGC 2 cut(s) 38, 110
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 5 cut(s) 147, 408, 690, 732, 796
FokI GGATG 2 cut(s) 198, 327
FriOI GRGCYC 2 cut(s) 480, 709
Fsp4HI GCNGC 5 cut(s) 147, 408, 690, 732, 796
FspBI CTAG 4 cut(s) 519, 693, 906, 910
GluI GCNGC 5 cut(s) 147, 408, 690, 732, 796
GsaI CCCAGC 1 cut(s) 572
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 4 cut(s) 101, 548, 618, 893
HapII CCGG 1 cut(s) 635
HgaI GACGC 1 cut(s) 800
Hin1I GRCGYC 1 cut(s) 811
Hin1II CATG 5 cut(s) 122, 344, 553, 713, 767
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 3 cut(s) 186, 464, 561
HpaII CCGG 1 cut(s) 635
HphI GGTGA 1 cut(s) 504
Hpy166II GTNNAC 2 cut(s) 85, 788
Hpy188I TCNGA 2 cut(s) 168, 543
Hpy188III TCNNGA 5 cut(s) 179, 203, 461, 635, 884
Hpy8I GTNNAC 2 cut(s) 85, 788
HpyAV CCTTC 3 cut(s) 47, 163, 538
HpyCH4III ACNGT 1 cut(s) 850
HpyCH4V TGCA 3 cut(s) 689, 734, 788
HpyF10VI GCNNNNNNNGC 2 cut(s) 124, 698
HpyF3I CTNAG 2 cut(s) 135, 244
Hsp92I GRCGYC 1 cut(s) 811
Hsp92II CATG 5 cut(s) 122, 344, 553, 713, 767
Kpn2I TCCGGA 1 cut(s) 634
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 3 cut(s) 297, 370, 457
LmnI GCTCC 2 cut(s) 64, 704
Lsp1109I GCAGC 5 cut(s) 133, 394, 701, 718, 807
LweI GCATC 2 cut(s) 129, 220
MaeI CTAG 4 cut(s) 519, 693, 906, 910
MaeIII GTNAC 3 cut(s) 239, 626, 816
MalI GATC 3 cut(s) 299, 372, 459
MbiI CCGCTC 1 cut(s) 11
MboI GATC 3 cut(s) 297, 370, 457
MboII GAAGA 1 cut(s) 495
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 4 cut(s) 358, 480, 709, 790
MlsI TGGCCA 1 cut(s) 893
MluCI AATT 5 cut(s) 108, 228, 301, 307, 444
MluNI TGGCCA 1 cut(s) 893
MmeI TCCRAC 1 cut(s) 703
MnlI CCTC 6 cut(s) 51, 210, 382, 603, 629, 673
Mox20I TGGCCA 1 cut(s) 893
MroI TCCGGA 1 cut(s) 634
MscI TGGCCA 1 cut(s) 893
MseI TTAA 5 cut(s) 306, 435, 597, 641, 738
MslI CAYNNNNRTG 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 893
MspA1I CMGCKG 2 cut(s) 572, 798
MspCI CTTAAG 1 cut(s) 640
MspI CCGG 1 cut(s) 635
MspR9I CCNGG 2 cut(s) 124, 254
MvaI CCWGG 2 cut(s) 124, 254
MwoI GCNNNNNNNGC 2 cut(s) 124, 698
NdeII GATC 3 cut(s) 297, 370, 457
NlaIII CATG 5 cut(s) 122, 344, 553, 713, 767
NlaIV GGNNCC 2 cut(s) 60, 372
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 1 cut(s) 626
NspI RCATGY 2 cut(s) 122, 767
PaeI GCATGC 1 cut(s) 122
PciI ACATGT 1 cut(s) 763
PfeI GAWTC 3 cut(s) 186, 464, 561
PkrI GCNGC 5 cut(s) 148, 409, 691, 733, 797
PscI ACATGT 1 cut(s) 763
PsiI TTATAA 1 cut(s) 495
Psp124BI GAGCTC 2 cut(s) 480, 709
Psp6I CCWGG 2 cut(s) 122, 252
PspFI CCCAGC 1 cut(s) 568
PspGI CCWGG 2 cut(s) 122, 252
PspN4I GGNNCC 2 cut(s) 60, 372
PspPI GGNCC 1 cut(s) 100
PsuI RGATCY 1 cut(s) 370
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 3 cut(s) 194, 608, 904
RsaNI GTAC 3 cut(s) 193, 607, 903
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 2 cut(s) 480, 709
SaqAI TTAA 5 cut(s) 306, 435, 597, 641, 738
SatI GCNGC 5 cut(s) 147, 408, 690, 732, 796
Sau3AI GATC 3 cut(s) 297, 370, 457
Sau96I GGNCC 1 cut(s) 100
ScaI AGTACT 2 cut(s) 608, 904
ScrFI CCNGG 2 cut(s) 124, 254
SduI GDGCHC 4 cut(s) 358, 480, 709, 790
SfaNI GCATC 2 cut(s) 129, 220
SfcI CTRYAG 1 cut(s) 88
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 2 cut(s) 640, 821
SmoI CTYRAG 2 cut(s) 640, 821
SphI GCATGC 1 cut(s) 122
Sse9I AATT 5 cut(s) 108, 228, 301, 307, 444
SsiI CCGC 4 cut(s) 9, 31, 103, 798
SspI AATATT 1 cut(s) 280
SspMI CTAG 4 cut(s) 519, 693, 906, 910
SstI GAGCTC 2 cut(s) 480, 709
StyD4I CCNGG 2 cut(s) 122, 252
StyI CCWWGG 2 cut(s) 44, 894
TaaI ACNGT 1 cut(s) 850
TaqI TCGA 3 cut(s) 442, 580, 883
TasI AATT 5 cut(s) 108, 228, 301, 307, 444
TatI WGTACW 3 cut(s) 192, 606, 902
TfiI GAWTC 3 cut(s) 186, 464, 561
Tru1I TTAA 5 cut(s) 306, 435, 597, 641, 738
Tru9I TTAA 5 cut(s) 306, 435, 597, 641, 738
TscAI CASTG 3 cut(s) 58, 139, 405
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 5 cut(s) 146, 407, 689, 731, 795
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 2 cut(s) 456, 517
TspRI CASTG 3 cut(s) 58, 139, 405
Vha464I CTTAAG 1 cut(s) 640
VneI GTGCAC 1 cut(s) 786
XceI RCATGY 2 cut(s) 122, 767
XspI CTAG 4 cut(s) 519, 693, 906, 910
ZrmI AGTACT 2 cut(s) 608, 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.