Rh1DG016600

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0008425
Physical Location & Seq
Reverse (-)
2733921 .. 2738469
4549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG016600.1

Sequence Viewer

Length: 1143 bp
ATGGTTTCCGCTCGCAACTACGCCCACGCCCACTACCTTCCTCCTTGGTTCAGTGTGGCTCCTATGATGGAATGGACTGATGTTCACTACAGGACAATGGCCCGCCTAATTTCCAAGCATGCCTGGCTCTACACTGAGATGCTCGCTGCCGAAACCATTGTCTATCAGAAGGACAATCTGGACAGATTCTTGTACTTTCCTCCAGAACAGCATCCTATTGTTCTACAAATTGGTGGCAGTAACTTAGAAAACCTGGCCAAAGCTACTCAACTTGCTAATATTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCCAGTCCAAGAGTAGCTGGACATGGGTGCTTCGGTGCTCGTCTTATGCTGGATCCAAAGTTTGTTGCCGAGGCTATGTCAGTGATTGCTGCGAACACAGATGTCCCTGTCAGCGTTAAGTGTCGAATTGGTGTTGATGATCACGATTCATATAATGAGCTCTGTGATTTTATTTATAAAGTTTCTTCTCAATCACCAACTAGGCACTTCATTATACATTCTCGGAAGGCCATGCTGAATGGAATCAGCCCAGCTGAAAATCGAAGTATTCCACCTCTGAAATACGAGTACTTTTATGGCCTCTTGCGTGACTTTCCGGACTTAAGATTTACCATAAATGGTGGAATAAACACTATTGATGAGGTCAATGCAGCTAGGAAAGCAGGAGCTCATGGTGTAATGGTTGGACGGGCTGCATTTAATAACTCATGGAATACTTTGGGACATGTTGATACTGCTATTTATGGTGCACCAAGCAGCGGTATAACACGGCGTCAGTTACTCAAGGAGTTTGAAGCTTATGGAGAGACTGTTTTGGGAAAATATGGACAAAAGCCCAATGTTCGAGATGTGGCCAAGCCTTTGCTTAGCCTTTTCTATGCTCAGCCTGGGAACAGCCTTTGGAAACGCAAAGCTGATGCTGCTTTCATGCATTGCACGACTATGAAATCATTTTTTGAGGAAACACTTGTAGCAATTCCAGACTTTGTCTTGGATGCACCTGTTGGAGCGCCACCATCTGGTAGTAATGATCCTTTTTCAAACATACATACTTTGTTGCCTCCACCATACGAATCAAGGGAACAAGAGCTACAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

380

Amino Acids

42.58

Weight (kDa)

6.67

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 19 - 326 1.8e-57 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
AccB7I CCANNNNNTGG 1 cut(s) 1058
AccBSI CCGCTC 1 cut(s) 11
AccIII TCCGGA 1 cut(s) 634
AciI CCGC 3 cut(s) 9, 103, 798
AclWI GGATC 3 cut(s) 365, 378, 1064
AcoI YGGCCR 2 cut(s) 255, 891
AcyI GRCGYC 1 cut(s) 811
AfaI GTAC 2 cut(s) 194, 608
AfiI CCNNNNNNNGG 2 cut(s) 797, 1058
AflII CTTAAG 1 cut(s) 640
AflIII ACRYGT 1 cut(s) 763
AgsI TTSAA 2 cut(s) 833, 1080
AjnI CCWGG 3 cut(s) 122, 252, 925
AluBI AGCT 9 cut(s) 263, 335, 478, 572, 692, 707, 836, 953, 1129
AluI AGCT 9 cut(s) 263, 335, 478, 572, 692, 707, 836, 953, 1129
Alw21I GWGCWC 4 cut(s) 358, 480, 709, 790
Alw26I GTCTC 1 cut(s) 839
Alw44I GTGCAC 1 cut(s) 786
AlwI GGATC 3 cut(s) 365, 378, 1064
Aor13HI TCCGGA 1 cut(s) 634
AoxI GGCC 5 cut(s) 99, 255, 546, 616, 891
ApaLI GTGCAC 1 cut(s) 786
ApeKI GCWGC 6 cut(s) 146, 407, 689, 731, 795, 959
ArsI GACNNNNNNTTYG 2 cut(s) 144, 176
AspLEI GCGC 1 cut(s) 1051
AspS9I GGNCC 1 cut(s) 100
AsuHPI GGTGA 1 cut(s) 504
BaeGI GKGCMC 1 cut(s) 790
BalI TGGCCA 2 cut(s) 257, 893
BamHI GGATCC 1 cut(s) 370
BanII GRGCYC 2 cut(s) 480, 709
Bbv12I GWGCWC 4 cut(s) 358, 480, 709, 790
BbvI GCAGC 6 cut(s) 133, 394, 701, 718, 807, 946
BccI CCATC 2 cut(s) 61, 1063
BceAI ACGGC 1 cut(s) 824
BciT130I CCWGG 3 cut(s) 124, 254, 927
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 839
BfaI CTAG 2 cut(s) 519, 693
BfmI CTRYAG 1 cut(s) 88
BfoI RGCGCY 1 cut(s) 1052
BfrI CTTAAG 1 cut(s) 640
BisI GCNGC 6 cut(s) 147, 408, 690, 732, 796, 960
BlpI GCTNAGC 2 cut(s) 905, 921
BlsI GCNGC 6 cut(s) 148, 409, 691, 733, 797, 961
BmcAI AGTACT 1 cut(s) 608
Bme1390I CCNGG 3 cut(s) 124, 254, 927
BmgT120I GGNCC 1 cut(s) 100
BmiI GGNNCC 2 cut(s) 60, 372
BmrFI CCNGG 3 cut(s) 124, 254, 927
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 4 cut(s) 129, 220, 946, 1024
BmuI ACTGGG 1 cut(s) 315
BpmI CTGGAG 1 cut(s) 186
Bpu1102I GCTNAGC 2 cut(s) 905, 921
BpuEI CTTGAG 1 cut(s) 806
BsaHI GRCGYC 1 cut(s) 811
BsaJI CCNNGG 3 cut(s) 44, 387, 926
BsaWI WCCGGW 1 cut(s) 634
BsaXI ACNNNNNCTCC 2 cut(s) 834, 864
Bsc4I CCNNNNNNNGG 2 cut(s) 797, 1058
Bse1I ACTGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 970
BseAI TCCGGA 1 cut(s) 634
BseBI CCWGG 3 cut(s) 124, 254, 927
BseDI CCNNGG 3 cut(s) 44, 387, 926
BseGI GGATG 3 cut(s) 211, 320, 1039
BseLI CCNNNNNNNGG 2 cut(s) 797, 1058
BseMI GCAATG 1 cut(s) 970
BseMII CTCAG 2 cut(s) 126, 935
BseNI ACTGG 1 cut(s) 321
BseSI GKGCMC 1 cut(s) 790
BseXI GCAGC 6 cut(s) 133, 394, 701, 718, 807, 946
BseYI CCCAGC 1 cut(s) 568
BshFI GGCC 5 cut(s) 101, 257, 548, 618, 893
BsiHKAI GWGCWC 4 cut(s) 358, 480, 709, 790
BsiSI CCGG 1 cut(s) 635
BslFI GGGAC 3 cut(s) 303, 407, 774
BslI CCNNNNNNNGG 2 cut(s) 797, 1058
BsmAI GTCTC 1 cut(s) 839
BsmFI GGGAC 3 cut(s) 303, 407, 774
BsnI GGCC 5 cut(s) 101, 257, 548, 618, 893
Bsp1286I GDGCHC 4 cut(s) 358, 480, 709, 790
Bsp13I TCCGGA 1 cut(s) 634
Bsp143I GATC 4 cut(s) 297, 370, 457, 1069
Bsp1720I GCTNAGC 2 cut(s) 905, 921
BspACI CCGC 3 cut(s) 9, 103, 798
BspANI GGCC 5 cut(s) 101, 257, 548, 618, 893
BspCNI CTCAG 2 cut(s) 127, 934
BspEI TCCGGA 1 cut(s) 634
BspLI GGNNCC 2 cut(s) 60, 372
BspPI GGATC 3 cut(s) 365, 378, 1064
BspTI CTTAAG 1 cut(s) 640
BsrBI CCGCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 970
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 3 cut(s) 44, 387, 926
BssMI GATC 4 cut(s) 297, 370, 457, 1069
BssNI GRCGYC 1 cut(s) 811
BssT1I CCWWGG 1 cut(s) 44
Bst2UI CCWGG 3 cut(s) 124, 254, 927
Bst4CI ACNGT 1 cut(s) 850
BstACI GRCGYC 1 cut(s) 811
BstAFI CTTAAG 1 cut(s) 640
BstC8I GCNNGC 4 cut(s) 13, 103, 120, 144
BstDEI CTNAG 5 cut(s) 135, 244, 905, 921, 1140
BstF5I GGATG 3 cut(s) 211, 320, 1039
BstH2I RGCGCY 1 cut(s) 1052
BstHHI GCGC 1 cut(s) 1051
BstKTI GATC 4 cut(s) 300, 373, 460, 1072
BstMAI GTCTC 1 cut(s) 839
BstMBI GATC 4 cut(s) 297, 370, 457, 1069
BstMWI GCNNNNNNNGC 3 cut(s) 124, 698, 959
BstNI CCWGG 3 cut(s) 124, 254, 927
BstNSI RCATGY 2 cut(s) 122, 767
BstSCI CCNGG 3 cut(s) 122, 252, 925
BstSFI CTRYAG 1 cut(s) 88
BstSLI GKGCMC 1 cut(s) 790
BstV1I GCAGC 6 cut(s) 133, 394, 701, 718, 807, 946
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 5 cut(s) 101, 257, 548, 618, 893
BtsCI GGATG 3 cut(s) 211, 320, 1039
BtsIMutI CAGTG 3 cut(s) 58, 132, 405
Cac8I GCNNGC 4 cut(s) 13, 103, 120, 144
CfoI GCGC 1 cut(s) 1051
Cfr13I GGNCC 1 cut(s) 100
CseI GACGC 1 cut(s) 800
Csp6I GTAC 2 cut(s) 193, 607
CviAII CATG 7 cut(s) 119, 341, 550, 710, 747, 764, 967
CviQI GTAC 2 cut(s) 193, 607
DdeI CTNAG 5 cut(s) 135, 244, 905, 921, 1140
DpnI GATC 4 cut(s) 299, 372, 459, 1071
DpnII GATC 4 cut(s) 297, 370, 457, 1069
EaeI YGGCCR 2 cut(s) 255, 891
Ecl136II GAGCTC 2 cut(s) 478, 707
Eco130I CCWWGG 1 cut(s) 44
Eco24I GRGCYC 2 cut(s) 480, 709
Eco53kI GAGCTC 2 cut(s) 478, 707
EcoICRI GAGCTC 2 cut(s) 478, 707
EcoRII CCWGG 3 cut(s) 122, 252, 925
EcoT14I CCWWGG 1 cut(s) 44
EcoT22I ATGCAT 1 cut(s) 972
EcoT38I GRGCYC 2 cut(s) 480, 709
ErhI CCWWGG 1 cut(s) 44
FaeI CATG 7 cut(s) 122, 344, 553, 713, 750, 767, 970
FaqI GGGAC 3 cut(s) 303, 407, 774
FatI CATG 7 cut(s) 118, 340, 549, 709, 746, 763, 966
FauI CCCGC 1 cut(s) 110
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 6 cut(s) 147, 408, 690, 732, 796, 960
FokI GGATG 3 cut(s) 198, 327, 1046
FriOI GRGCYC 2 cut(s) 480, 709
Fsp4HI GCNGC 6 cut(s) 147, 408, 690, 732, 796, 960
FspBI CTAG 2 cut(s) 519, 693
GlaI GCGC 1 cut(s) 1050
GluI GCNGC 6 cut(s) 147, 408, 690, 732, 796, 960
GsaI CCCAGC 1 cut(s) 572
GsuI CTGGAG 1 cut(s) 186
HaeII RGCGCY 1 cut(s) 1052
HaeIII GGCC 5 cut(s) 101, 257, 548, 618, 893
HapII CCGG 1 cut(s) 635
HgaI GACGC 1 cut(s) 800
HhaI GCGC 1 cut(s) 1051
Hin1I GRCGYC 1 cut(s) 811
Hin1II CATG 7 cut(s) 122, 344, 553, 713, 750, 767, 970
Hin6I GCGC 1 cut(s) 1049
HinP1I GCGC 1 cut(s) 1049
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 4 cut(s) 186, 464, 561, 1112
HpaII CCGG 1 cut(s) 635
HphI GGTGA 1 cut(s) 504
Hpy166II GTNNAC 2 cut(s) 85, 788
Hpy188I TCNGA 3 cut(s) 168, 543, 597
Hpy188III TCNNGA 6 cut(s) 179, 203, 461, 635, 884, 1019
Hpy8I GTNNAC 2 cut(s) 85, 788
HpyAV CCTTC 3 cut(s) 47, 163, 538
HpyCH4III ACNGT 1 cut(s) 850
HpyCH4V TGCA 6 cut(s) 689, 734, 788, 970, 975, 1037
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 698, 959
HpyF3I CTNAG 5 cut(s) 135, 244, 905, 921, 1140
Hsp92I GRCGYC 1 cut(s) 811
Hsp92II CATG 7 cut(s) 122, 344, 553, 713, 750, 767, 970
HspAI GCGC 1 cut(s) 1049
Kpn2I TCCGGA 1 cut(s) 634
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 4 cut(s) 297, 370, 457, 1069
LmnI GCTCC 3 cut(s) 64, 704, 1046
Lsp1109I GCAGC 6 cut(s) 133, 394, 701, 718, 807, 946
LweI GCATC 4 cut(s) 129, 220, 946, 1024
MaeI CTAG 2 cut(s) 519, 693
MaeIII GTNAC 3 cut(s) 239, 626, 816
MalI GATC 4 cut(s) 299, 372, 459, 1071
MbiI CCGCTC 1 cut(s) 11
MboI GATC 4 cut(s) 297, 370, 457, 1069
MboII GAAGA 1 cut(s) 495
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 4 cut(s) 358, 480, 709, 790
MlsI TGGCCA 2 cut(s) 257, 893
MluCI AATT 6 cut(s) 108, 228, 301, 307, 444, 1014
MluNI TGGCCA 2 cut(s) 257, 893
MmeI TCCRAC 2 cut(s) 703, 1024
MnlI CCTC 8 cut(s) 51, 210, 382, 603, 629, 673, 991, 1110
Mox20I TGGCCA 2 cut(s) 257, 893
Mph1103I ATGCAT 1 cut(s) 972
MroI TCCGGA 1 cut(s) 634
MscI TGGCCA 2 cut(s) 257, 893
MseI TTAA 4 cut(s) 306, 435, 641, 738
MslI CAYNNNNRTG 2 cut(s) 137, 980
Msp20I TGGCCA 2 cut(s) 257, 893
MspA1I CMGCKG 2 cut(s) 572, 798
MspCI CTTAAG 1 cut(s) 640
MspI CCGG 1 cut(s) 635
MspR9I CCNGG 3 cut(s) 124, 254, 927
MvaI CCWGG 3 cut(s) 124, 254, 927
MwoI GCNNNNNNNGC 3 cut(s) 124, 698, 959
NdeII GATC 4 cut(s) 297, 370, 457, 1069
NlaIII CATG 7 cut(s) 122, 344, 553, 713, 750, 767, 970
NlaIV GGNNCC 2 cut(s) 60, 372
NmeAIII GCCGAG 1 cut(s) 412
NmuCI GTSAC 1 cut(s) 626
NsiI ATGCAT 1 cut(s) 972
NspI RCATGY 2 cut(s) 122, 767
PaeI GCATGC 1 cut(s) 122
PciI ACATGT 1 cut(s) 763
PfeI GAWTC 4 cut(s) 186, 464, 561, 1112
PflFI GACNNNGTC 1 cut(s) 1025
PflMI CCANNNNNTGG 1 cut(s) 1058
PkrI GCNGC 6 cut(s) 148, 409, 691, 733, 797, 961
PscI ACATGT 1 cut(s) 763
PsiI TTATAA 1 cut(s) 495
Psp124BI GAGCTC 2 cut(s) 480, 709
Psp6I CCWGG 3 cut(s) 122, 252, 925
PspFI CCCAGC 1 cut(s) 568
PspGI CCWGG 3 cut(s) 122, 252, 925
PspN4I GGNNCC 2 cut(s) 60, 372
PspPI GGNCC 1 cut(s) 100
PsrI GAACNNNNNNTAC 1 cut(s) 1113
PsuI RGATCY 1 cut(s) 370
PsyI GACNNNGTC 1 cut(s) 1025
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 2 cut(s) 194, 608
RsaNI GTAC 2 cut(s) 193, 607
RseI CAYNNNNRTG 2 cut(s) 137, 980
SacI GAGCTC 2 cut(s) 480, 709
SaqAI TTAA 4 cut(s) 306, 435, 641, 738
SatI GCNGC 6 cut(s) 147, 408, 690, 732, 796, 960
Sau3AI GATC 4 cut(s) 297, 370, 457, 1069
Sau96I GGNCC 1 cut(s) 100
ScaI AGTACT 1 cut(s) 608
ScrFI CCNGG 3 cut(s) 124, 254, 927
SduI GDGCHC 4 cut(s) 358, 480, 709, 790
SfaNI GCATC 4 cut(s) 129, 220, 946, 1024
SfcI CTRYAG 1 cut(s) 88
SmiMI CAYNNNNRTG 2 cut(s) 137, 980
SmlI CTYRAG 2 cut(s) 640, 821
SmoI CTYRAG 2 cut(s) 640, 821
SphI GCATGC 1 cut(s) 122
Sse9I AATT 6 cut(s) 108, 228, 301, 307, 444, 1014
SsiI CCGC 3 cut(s) 9, 103, 798
SspI AATATT 1 cut(s) 280
SspMI CTAG 2 cut(s) 519, 693
SstI GAGCTC 2 cut(s) 480, 709
StyD4I CCNGG 3 cut(s) 122, 252, 925
StyI CCWWGG 1 cut(s) 44
TaaI ACNGT 1 cut(s) 850
TaqI TCGA 3 cut(s) 442, 580, 883
TasI AATT 6 cut(s) 108, 228, 301, 307, 444, 1014
TatI WGTACW 2 cut(s) 192, 606
TfiI GAWTC 4 cut(s) 186, 464, 561, 1112
Tru1I TTAA 4 cut(s) 306, 435, 641, 738
Tru9I TTAA 4 cut(s) 306, 435, 641, 738
TscAI CASTG 3 cut(s) 58, 139, 405
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 6 cut(s) 146, 407, 689, 731, 795, 959
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 4 cut(s) 456, 517, 955, 998
TspRI CASTG 3 cut(s) 58, 139, 405
Tth111I GACNNNGTC 1 cut(s) 1025
Van91I CCANNNNNTGG 1 cut(s) 1058
Vha464I CTTAAG 1 cut(s) 640
VneI GTGCAC 1 cut(s) 786
XceI RCATGY 2 cut(s) 122, 767
XspI CTAG 2 cut(s) 519, 693
ZrmI AGTACT 1 cut(s) 608
Zsp2I ATGCAT 1 cut(s) 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.