Rorug01G0011800

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008425
Physical Location & Seq
Forward (+)
1967098 .. 1968350
1253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0011800.1

Sequence Viewer

Length: 1221 bp
ATGGCTCAACTCTCCAAACTCAATGTTATTGAGCAATGGCAAGTCTCTACCCCGCAAAACTCATCAACTCCACAAGCTTGTTATCACCTCCCTCTAACCTTCCTTGACATACCATGGCTCTTCTTCTCTCCAAGCCAACCTCTTTTCTTCTATGAATTCCCATATCCCACTTCTCATTTCACATCGATCACTCTACCAAACCTCAAGCACTCTCTCTCCCTTACTCTCCAACTCTTTTACCCTTTTGCCGGAGCCCTACTGGCCCGCCCCGAACCCGGAAAACCCGAGCTCGTCTACTCCCAAGGTGACTCGGTCTCATTCACCGTCGTCGAGTCAAATGCAGACTTCGGCTATCTTTCCGGGAATTACCAAAGAGATGTTGAAGAGTTCAACACACTTGTACCTCAACTAGCTTCAACTGGTGCACCTAGTCGTAACTGTGCAGCCAAAATTCCATTGTTGAGCGTTCAAATTACATTGTTCCCAAATTGTGGCATTTGCATTGGACTTGCATATCATCATGTTGTTGCTGACAAGAGAACGTTCAACAATTTCATCAAGACTTGGGCCTCATTTTCTAGACATTCATTTTTCCCCACCAAGTCTATCTTGCCCTCTTATGATCGTAAGGCCATTGTTGATCCGTATAAGTTGGAGGGTATCTTCTTGAAGGAATGGTGGAAGCGAATTTCTTCTCAAGGAGCAATTATAGGTAATACTAAAACTGCCATGTTAGATCCAACGAGCATGGTTCGGGCAACTTTCTTGATGGACTTGACCCGCATGGAGAGGATCAAAAATTGGATTGTTAATCTATGCAATAAGGGAAATGAGCCACTACCGGTACATTTATCTGCTTATGTCCTAACATGTGCGTTTGTATGGGTTTCTTTGATTAAAAGCCAAGAAAAATACTTCAATTACAAGTGTTGTGGTCAGGATCAGAATTATTTTGGGTTTATTGCGGGTGGGTTAACCCGTTTGAACCATCCGGTACCTTCAACGTATGTCGGTAATTGTGTAGGGTTTGGCCGGGCAGCGGCAATAAGGAGTGAGTTGTTGGGGGACAATGGAATTGTTGTTGCAGCAAGAGCGATTGGAAATACGGTTAAGAAGTTAAACAAGGCTATTATTGGAATAAATCACATGCTGTCTACTTTGTTGTCCACCTTCCTGTACATAGATGCCTTCCACTTTGCTGCAATCTGCTTTCATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

406

Amino Acids

45.48

Weight (kDa)

8.98

Isoelectric Point (pI)

31.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 23 - 374 3.7e-28 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 994
AccB1I GGYRCC 1 cut(s) 994
AccB7I CCANNNNNTGG 1 cut(s) 491
AccI GTMKAC 2 cut(s) 294, 1154
AciI CCGC 5 cut(s) 53, 265, 781, 965, 1040
AclI AACGTT 1 cut(s) 542
AclWI GGATC 4 cut(s) 635, 731, 800, 948
AcoI YGGCCR 1 cut(s) 1030
AcsI RAATTY 3 cut(s) 155, 450, 687
AfaI GTAC 4 cut(s) 402, 846, 996, 1178
AfiI CCNNNNNNNGG 4 cut(s) 248, 275, 491, 1039
AflIII ACRYGT 1 cut(s) 869
AgeI ACCGGT 1 cut(s) 841
AgsI TTSAA 9 cut(s) 383, 391, 417, 470, 547, 670, 919, 985, 1002
AluBI AGCT 3 cut(s) 77, 289, 413
AluI AGCT 3 cut(s) 77, 289, 413
Alw21I GWGCWC 2 cut(s) 291, 427
Alw26I GTCTC 2 cut(s) 49, 319
Alw44I GTGCAC 1 cut(s) 423
AlwI GGATC 4 cut(s) 635, 731, 800, 948
Ama87I CYCGRG 1 cut(s) 284
AoxI GGCC 4 cut(s) 261, 567, 630, 1030
ApaLI GTGCAC 1 cut(s) 423
ApeKI GCWGC 4 cut(s) 443, 1037, 1085, 1199
ApoI RAATTY 3 cut(s) 155, 450, 687
AsiGI ACCGGT 1 cut(s) 841
Asp700I GAANNNNTTC 1 cut(s) 691
Asp718I GGTACC 1 cut(s) 994
AspS9I GGNCC 2 cut(s) 262, 567
AsuC2I CCSGG 3 cut(s) 276, 361, 1034
AsuHPI GGTGA 3 cut(s) 77, 313, 317
AvaI CYCGRG 1 cut(s) 284
BaeGI GKGCMC 1 cut(s) 427
BaeI ACNNNNGTAYC 2 cut(s) 384, 417
BanI GGYRCC 1 cut(s) 994
BanII GRGCYC 2 cut(s) 256, 291
Bbv12I GWGCWC 2 cut(s) 291, 427
BbvI GCAGC 4 cut(s) 455, 1049, 1097, 1186
BccI CCATC 2 cut(s) 763, 996
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BcnI CCSGG 3 cut(s) 276, 361, 1034
BcoDI GTCTC 2 cut(s) 49, 319
BfaI CTAG 3 cut(s) 410, 429, 579
BglI GCCNNNNNGGC 1 cut(s) 260
BisI GCNGC 5 cut(s) 444, 1038, 1041, 1086, 1200
BlsI GCNGC 5 cut(s) 445, 1039, 1042, 1087, 1201
Bme1390I CCNGG 3 cut(s) 276, 361, 1034
BmeT110I CYCGRG 1 cut(s) 284
BmgT120I GGNCC 2 cut(s) 262, 567
BmiI GGNNCC 2 cut(s) 253, 996
BmrFI CCNGG 3 cut(s) 276, 361, 1034
BmsI GCATC 1 cut(s) 1174
BpuEI CTTGAG 2 cut(s) 188, 681
BpuMI CCSGG 3 cut(s) 276, 361, 1034
Bsa29I ATCGAT 1 cut(s) 185
BsaI GGTCTC 1 cut(s) 319
BsaJI CCNNGG 2 cut(s) 113, 301
BsaWI WCCGGW 2 cut(s) 841, 991
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 4 cut(s) 248, 275, 491, 1039
Bse118I RCCGGY 1 cut(s) 841
Bse1I ACTGG 2 cut(s) 264, 424
Bse3DI GCAATG 1 cut(s) 41
BseCI ATCGAT 1 cut(s) 185
BseDI CCNNGG 2 cut(s) 113, 301
BseGI GGATG 1 cut(s) 988
BseLI CCNNNNNNNGG 4 cut(s) 248, 275, 491, 1039
BseMI GCAATG 1 cut(s) 41
BseNI ACTGG 2 cut(s) 264, 424
BseSI GKGCMC 1 cut(s) 427
BseXI GCAGC 4 cut(s) 455, 1049, 1097, 1186
BsgI GTGCAG 1 cut(s) 462
BshFI GGCC 4 cut(s) 263, 569, 632, 1032
BshNI GGYRCC 1 cut(s) 994
BshTI ACCGGT 1 cut(s) 841
BshVI ATCGAT 1 cut(s) 185
BsiHKAI GWGCWC 2 cut(s) 291, 427
BsiHKCI CYCGRG 1 cut(s) 284
BsiSI CCGG 6 cut(s) 249, 276, 360, 842, 992, 1033
BslFI GGGAC 1 cut(s) 1079
BslI CCNNNNNNNGG 4 cut(s) 248, 275, 491, 1039
BsmAI GTCTC 2 cut(s) 49, 319
BsmFI GGGAC 1 cut(s) 1079
BsnI GGCC 4 cut(s) 263, 569, 632, 1032
Bso31I GGTCTC 1 cut(s) 319
BsoBI CYCGRG 1 cut(s) 284
Bsp1286I GDGCHC 3 cut(s) 256, 291, 427
Bsp1407I TGTACA 1 cut(s) 1176
Bsp143I GATC 6 cut(s) 186, 622, 640, 736, 792, 940
Bsp19I CCATGG 1 cut(s) 113
BspACI CCGC 5 cut(s) 53, 265, 781, 965, 1040
BspANI GGCC 4 cut(s) 263, 569, 632, 1032
BspDI ATCGAT 1 cut(s) 185
BspLI GGNNCC 2 cut(s) 253, 996
BspPI GGATC 4 cut(s) 635, 731, 800, 948
BspQI GCTCTTC 1 cut(s) 125
BspT107I GGYRCC 1 cut(s) 994
BspTNI GGTCTC 1 cut(s) 319
BsrDI GCAATG 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 841
BsrGI TGTACA 1 cut(s) 1176
BsrI ACTGG 2 cut(s) 264, 424
BssAI RCCGGY 1 cut(s) 841
BssECI CCNNGG 2 cut(s) 113, 301
BssMI GATC 6 cut(s) 186, 622, 640, 736, 792, 940
BssT1I CCWWGG 2 cut(s) 113, 301
Bst4CI ACNGT 3 cut(s) 325, 440, 1108
Bst6I CTCTTC 2 cut(s) 125, 378
BstAUI TGTACA 1 cut(s) 1176
BstC8I GCNNGC 1 cut(s) 265
BstDSI CCRYGG 1 cut(s) 113
BstF5I GGATG 1 cut(s) 988
BstKTI GATC 6 cut(s) 189, 625, 643, 739, 795, 943
BstMAI GTCTC 2 cut(s) 49, 319
BstMBI GATC 6 cut(s) 186, 622, 640, 736, 792, 940
BstMWI GCNNNNNNNGC 2 cut(s) 260, 1091
BstNSI RCATGY 2 cut(s) 873, 1150
BstSCI CCNGG 3 cut(s) 274, 359, 1032
BstSLI GKGCMC 1 cut(s) 427
BstV1I GCAGC 4 cut(s) 455, 1049, 1097, 1186
BstX2I RGATCY 1 cut(s) 736
BstYI RGATCY 1 cut(s) 736
Bsu15I ATCGAT 1 cut(s) 185
BsuRI GGCC 4 cut(s) 263, 569, 632, 1032
BsuTUI ATCGAT 1 cut(s) 185
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 1 cut(s) 988
Cac8I GCNNGC 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 841
Cfr13I GGNCC 2 cut(s) 262, 567
ClaI ATCGAT 1 cut(s) 185
Csp6I GTAC 4 cut(s) 401, 845, 995, 1177
CspAI ACCGGT 1 cut(s) 841
CspCI CAANNNNNGTGG 2 cut(s) 913, 948
CviAII CATG 7 cut(s) 114, 521, 730, 748, 784, 870, 1147
CviQI GTAC 4 cut(s) 401, 845, 995, 1177
DpnI GATC 6 cut(s) 188, 624, 642, 738, 794, 942
DpnII GATC 6 cut(s) 186, 622, 640, 736, 792, 940
EaeI YGGCCR 1 cut(s) 1030
Eam1104I CTCTTC 2 cut(s) 125, 378
EarI CTCTTC 2 cut(s) 125, 378
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 2 cut(s) 113, 301
Eco24I GRGCYC 2 cut(s) 256, 291
Eco31I GGTCTC 1 cut(s) 319
Eco53kI GAGCTC 1 cut(s) 289
Eco88I CYCGRG 1 cut(s) 284
EcoICRI GAGCTC 1 cut(s) 289
EcoRI GAATTC 1 cut(s) 155
EcoT14I CCWWGG 2 cut(s) 113, 301
EcoT38I GRGCYC 2 cut(s) 256, 291
ErhI CCWWGG 2 cut(s) 113, 301
FaeI CATG 7 cut(s) 117, 524, 733, 751, 787, 873, 1150
FalI AAGNNNNNCTT 2 cut(s) 593, 625
FaqI GGGAC 1 cut(s) 1079
FatI CATG 7 cut(s) 113, 520, 729, 747, 783, 869, 1146
FauI CCCGC 4 cut(s) 60, 272, 788, 958
FblI GTMKAC 2 cut(s) 294, 1154
Fnu4HI GCNGC 5 cut(s) 444, 1038, 1041, 1086, 1200
FokI GGATG 1 cut(s) 975
FriOI GRGCYC 2 cut(s) 256, 291
Fsp4HI GCNGC 5 cut(s) 444, 1038, 1041, 1086, 1200
FspBI CTAG 3 cut(s) 410, 429, 579
GluI GCNGC 5 cut(s) 444, 1038, 1041, 1086, 1200
HaeIII GGCC 4 cut(s) 263, 569, 632, 1032
HapII CCGG 6 cut(s) 249, 276, 360, 842, 992, 1033
Hin1II CATG 7 cut(s) 117, 524, 733, 751, 787, 873, 1150
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 2 cut(s) 308, 332
HpaI GTTAAC 1 cut(s) 975
HpaII CCGG 6 cut(s) 249, 276, 360, 842, 992, 1033
HphI GGTGA 3 cut(s) 77, 313, 317
Hpy166II GTNNAC 5 cut(s) 295, 425, 975, 1155, 1167
Hpy188I TCNGA 1 cut(s) 945
Hpy188III TCNNGA 5 cut(s) 559, 579, 667, 766, 938
Hpy8I GTNNAC 5 cut(s) 295, 425, 975, 1155, 1167
Hpy99I CGWCG 2 cut(s) 329, 332
HpyAV CCTTC 5 cut(s) 109, 664, 1008, 1180, 1198
HpyCH4III ACNGT 3 cut(s) 325, 440, 1108
HpyCH4IV ACGT 2 cut(s) 542, 1004
HpyCH4V TGCA 8 cut(s) 341, 425, 443, 501, 512, 819, 1085, 1202
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 1091
HpySE526I ACGT 2 cut(s) 542, 1004
Hsp92II CATG 7 cut(s) 117, 524, 733, 751, 787, 873, 1150
KpnI GGTACC 1 cut(s) 998
KspAI GTTAAC 1 cut(s) 975
Kzo9I GATC 6 cut(s) 186, 622, 640, 736, 792, 940
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 2 cut(s) 251, 701
Lsp1109I GCAGC 4 cut(s) 455, 1049, 1097, 1186
LweI GCATC 1 cut(s) 1174
MaeI CTAG 3 cut(s) 410, 429, 579
MaeII ACGT 2 cut(s) 542, 1004
MaeIII GTNAC 2 cut(s) 305, 434
MalI GATC 6 cut(s) 188, 624, 642, 738, 794, 942
MboI GATC 6 cut(s) 186, 622, 640, 736, 792, 940
MboII GAAGA 6 cut(s) 112, 115, 139, 395, 655, 684
MflI RGATCY 1 cut(s) 736
MhlI GDGCHC 3 cut(s) 256, 291, 427
MlyI GAGTC 2 cut(s) 302, 341
MmeI TCCRAC 3 cut(s) 253, 633, 764
MnlI CCTC 9 cut(s) 98, 102, 150, 212, 414, 580, 625, 649, 783
MroXI GAANNNNTTC 1 cut(s) 691
MseI TTAA 6 cut(s) 810, 897, 974, 1110, 1118, 1219
MspA1I CMGCKG 1 cut(s) 1040
MspI CCGG 6 cut(s) 249, 276, 360, 842, 992, 1033
MspR9I CCNGG 3 cut(s) 276, 361, 1034
MwoI GCNNNNNNNGC 2 cut(s) 260, 1091
NciI CCSGG 3 cut(s) 276, 361, 1034
NcoI CCATGG 1 cut(s) 113
NdeII GATC 6 cut(s) 186, 622, 640, 736, 792, 940
NlaIII CATG 7 cut(s) 117, 524, 733, 751, 787, 873, 1150
NlaIV GGNNCC 2 cut(s) 253, 996
NmuCI GTSAC 1 cut(s) 305
NspI RCATGY 2 cut(s) 873, 1150
PciI ACATGT 1 cut(s) 869
PciSI GCTCTTC 1 cut(s) 125
PdmI GAANNNNTTC 1 cut(s) 691
PflFI GACNNNGTC 1 cut(s) 311
PflMI CCANNNNNTGG 1 cut(s) 491
PfoI TCCNGGA 1 cut(s) 359
PinAI ACCGGT 1 cut(s) 841
PkrI GCNGC 5 cut(s) 445, 1039, 1042, 1087, 1201
PleI GAGTC 2 cut(s) 302, 340
PpsI GAGTC 2 cut(s) 302, 340
PscI ACATGT 1 cut(s) 869
Psp124BI GAGCTC 1 cut(s) 291
Psp1406I AACGTT 1 cut(s) 542
PspN4I GGNNCC 2 cut(s) 253, 996
PspPI GGNCC 2 cut(s) 262, 567
PsuI RGATCY 1 cut(s) 736
PsyI GACNNNGTC 1 cut(s) 311
RsaI GTAC 4 cut(s) 402, 846, 996, 1178
RsaNI GTAC 4 cut(s) 401, 845, 995, 1177
SacI GAGCTC 1 cut(s) 291
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 6 cut(s) 810, 897, 974, 1110, 1118, 1219
SatI GCNGC 5 cut(s) 444, 1038, 1041, 1086, 1200
Sau3AI GATC 6 cut(s) 186, 622, 640, 736, 792, 940
Sau96I GGNCC 2 cut(s) 262, 567
SchI GAGTC 2 cut(s) 302, 341
ScrFI CCNGG 3 cut(s) 276, 361, 1034
SduI GDGCHC 3 cut(s) 256, 291, 427
SfaNI GCATC 1 cut(s) 1174
SmlI CTYRAG 2 cut(s) 203, 696
SmoI CTYRAG 2 cut(s) 203, 696
SsiI CCGC 5 cut(s) 53, 265, 781, 965, 1040
SspMI CTAG 3 cut(s) 410, 429, 579
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 3 cut(s) 274, 359, 1032
StyI CCWWGG 2 cut(s) 113, 301
TaaI ACNGT 3 cut(s) 325, 440, 1108
TaiI ACGT 2 cut(s) 545, 1007
TaqI TCGA 2 cut(s) 185, 330
TaqII GACCGA 1 cut(s) 301
TatI WGTACW 1 cut(s) 1176
TauI GCSGC 1 cut(s) 1043
Tru1I TTAA 6 cut(s) 810, 897, 974, 1110, 1118, 1219
Tru9I TTAA 6 cut(s) 810, 897, 974, 1110, 1118, 1219
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 4 cut(s) 443, 1037, 1085, 1199
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 4 cut(s) 168, 544, 576, 1202
TspGWI ACGGA 1 cut(s) 633
Tth111I GACNNNGTC 1 cut(s) 311
Van91I CCANNNNNTGG 1 cut(s) 491
VneI GTGCAC 1 cut(s) 423
XapI RAATTY 3 cut(s) 155, 450, 687
XbaI TCTAGA 1 cut(s) 578
XceI RCATGY 2 cut(s) 873, 1150
XmiI GTMKAC 2 cut(s) 294, 1154
XmnI GAANNNNTTC 1 cut(s) 691
XspI CTAG 3 cut(s) 410, 429, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.