MD02G1308500.v1.1

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008425
Physical Location & Seq
Reverse (-)
36379635 .. 36385307
5673 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1308500.v1.1.491

Sequence Viewer

Length: 1377 bp
ATGAAGTTTCCAACTTCTTCTTCTTCGTATTCGTATTCGTATTCCTCAGTTACTCCTATTCATTCAGTTTTTCTTCGCACAAAAAACCGTGGCAAATTGTCGTTTAATCTATTTCAGGGCACTTCTTCTTCAACAACCACCGCATCCACCACCACCTCCACTTCCACTTCCTCCTCCGCCTCTCAAAATCATCAACTCCTCAGCGGTAGCATACCCGCCGCCGCCGCCATGGTTTCTTCTCCCGCCACCCACCCCCACCACGCCCACTACCCTCCTCCTTTGTTTAGTGTTGCCCCGATGATGGAGTGGACTGACAATCACTACAGGACTTTGGCCCGGCTTATTTCGAAGAATGCCTGGCTCTACACTGAGATGCTTGCTGCTGAAACCATTGTTTACCAGAAGGATAATCTGGACAGATTCTTGGAATATTCTCCAGAACAACATCCTATTGTTCTTCAAATTGGCGGGAATAATTTGGAAAACCTCGCCAAAGCGACTGAGCTTGCTAACCCTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCAAGTCCAAAAGTAGCTGGGCACGGGTGCTTTGGTGCTCGTCTTATGCTTGATCCAAAGTTTGTTGCTGAGGCTATGTCAGTAATTGCTGCGCACACAGATGTACCTGTCAGCGTTAAGTGTCGAATTGGTGTTGATGATCATGATTCATATAACGAGCTCTGTGATTTTATTTACAAGGTTTCTTCTCAATCTCCAACTAGGCATTTCATAATACATTCACGGAAGGCACTGCTCAATGGAATTAGCCCGGCTGAAAATCGAAGTATTCCGCCTCTTAAATATGAGTACTTTTATGGCCTTTTGCGTGACTTTCCAGACTTGAGATTTACAATAAATGGGGGCATAAACACCGTTGAGGAGGTCAATGCAGCAAGAAGGGCAGGAGCTCATGGTGTAATGGTTGGACGCGCTGCATATAATAAACCATGGCATACTCTGGGACATGTTGATACTGTAGTTTATGGAGCACCAAGCAGTGGTTTAACGCGGCGTCAGATACTGGAGAAGTTTCAAGTGTATGGAGATACTGCTGTGGGAAGATATGGACGCAAACCAACTGTGCGAGATGTAGCAAGGCCTTTGGTGGGTCTCTTCCATTCGGAACCTGGGAATGGTCTATGGAAACGCAAAGCTGATGCTGCTTTCCAGCGTTGCACGACTATGAAAGAGTTTTTTGATGAAACGCTTGTAGCACTTCCTGACTATGTTTTGGATAAACCTATTGGGGAGCCACCATCACACAGTGACAATCCTTTCGCGAACATACACAGTTTGCTACCTCCGGCGTATGAATCAAGGGAACTTGAACTGCAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

459

Amino Acids

50.75

Weight (kDa)

8.11

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 97 - 405 2.7e-59 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 645
AccB7I CCANNNNNTGG 1 cut(s) 1031
AccII CGCG 3 cut(s) 963, 1042, 1313
AclWI GGATC 1 cut(s) 599
AcyI GRCGYC 1 cut(s) 1045
AdeI CACNNNGTG 1 cut(s) 1298
AfaI GTAC 2 cut(s) 657, 842
AfiI CCNNNNNNNGG 4 cut(s) 301, 1031, 1139, 1166
AflIII ACRYGT 1 cut(s) 997
AgsI TTSAA 4 cut(s) 132, 461, 1067, 1361
AjnI CCWGG 2 cut(s) 356, 1159
AjuI GAANNNNNNNTTGG 1 cut(s) 36
AluBI AGCT 5 cut(s) 505, 569, 712, 941, 1187
AluI AGCT 5 cut(s) 505, 569, 712, 941, 1187
Alw21I GWGCWC 4 cut(s) 592, 714, 943, 1024
Alw26I GTCTC 1 cut(s) 1148
AlwI GGATC 1 cut(s) 599
AlwNI CAGNNNCTG 1 cut(s) 1054
AoxI GGCC 3 cut(s) 333, 850, 1130
ApeKI GCWGC 5 cut(s) 380, 641, 923, 965, 1193
ArsI GACNNNNNNTTYG 2 cut(s) 1292, 1324
AspLEI GCGC 2 cut(s) 646, 965
AspS9I GGNCC 1 cut(s) 334
AsuC2I CCSGG 2 cut(s) 337, 803
AsuII TTCGAA 1 cut(s) 347
BaeGI GKGCMC 2 cut(s) 122, 576
BaeI ACNNNNGTAYC 6 cut(s) 639, 672, 996, 1029, 1071, 1104
BanII GRGCYC 2 cut(s) 714, 943
Bbv12I GWGCWC 4 cut(s) 592, 714, 943, 1024
BbvCI CCTCAGC 2 cut(s) 200, 621
BbvI GCAGC 5 cut(s) 367, 628, 935, 952, 1180
BccI CCATC 2 cut(s) 295, 1297
BciT130I CCWGG 2 cut(s) 358, 1161
BclI TGATCA 1 cut(s) 691
BcnI CCSGG 2 cut(s) 337, 803
BcoDI GTCTC 1 cut(s) 1148
BfaI CTAG 1 cut(s) 753
BfmI CTRYAG 2 cut(s) 322, 1008
BisI GCNGC 9 cut(s) 219, 222, 225, 381, 642, 924, 966, 1043, 1194
BlsI GCNGC 9 cut(s) 220, 223, 226, 382, 643, 925, 967, 1044, 1195
BmcAI AGTACT 1 cut(s) 842
Bme1390I CCNGG 4 cut(s) 337, 358, 803, 1161
BmgT120I GGNCC 1 cut(s) 334
BmiI GGNNCC 2 cut(s) 1158, 1284
BmrFI CCNGG 4 cut(s) 337, 358, 803, 1161
BmsI GCATC 3 cut(s) 152, 363, 1180
BpmI CTGGAG 2 cut(s) 420, 1076
Bpu10I CCTNAGC 2 cut(s) 200, 621
Bpu14I TTCGAA 1 cut(s) 347
BpuEI CTTGAG 1 cut(s) 895
BpuMI CCSGG 2 cut(s) 337, 803
BsaHI GRCGYC 1 cut(s) 1045
BsaI GGTCTC 1 cut(s) 1148
BsaJI CCNNGG 4 cut(s) 88, 228, 980, 1160
Bsc4I CCNNNNNNNGG 4 cut(s) 301, 1031, 1139, 1166
Bse1I ACTGG 1 cut(s) 1059
BseBI CCWGG 2 cut(s) 358, 1161
BseDI CCNNGG 4 cut(s) 88, 228, 980, 1160
BseGI GGATG 3 cut(s) 143, 445, 554
BseLI CCNNNNNNNGG 4 cut(s) 301, 1031, 1139, 1166
BseMII CTCAG 5 cut(s) 60, 214, 360, 492, 612
BseNI ACTGG 1 cut(s) 1059
BseRI GAGGAG 4 cut(s) 163, 188, 264, 926
BseSI GKGCMC 2 cut(s) 122, 576
BseXI GCAGC 5 cut(s) 367, 628, 935, 952, 1180
BseYI CCCAGC 1 cut(s) 569
Bsh1236I CGCG 3 cut(s) 963, 1042, 1313
BshFI GGCC 3 cut(s) 335, 852, 1132
BsiHKAI GWGCWC 4 cut(s) 592, 714, 943, 1024
BsiSI CCGG 3 cut(s) 337, 803, 1337
BslFI GGGAC 1 cut(s) 1008
BslI CCNNNNNNNGG 4 cut(s) 301, 1031, 1139, 1166
BsmAI GTCTC 1 cut(s) 1148
BsmFI GGGAC 1 cut(s) 1008
BsmI GAATGC 1 cut(s) 358
BsnI GGCC 3 cut(s) 335, 852, 1132
Bso31I GGTCTC 1 cut(s) 1148
Bsp119I TTCGAA 1 cut(s) 347
Bsp1286I GDGCHC 6 cut(s) 122, 576, 592, 714, 943, 1024
Bsp143I GATC 3 cut(s) 531, 604, 691
Bsp19I CCATGG 2 cut(s) 228, 980
Bsp68I TCGCGA 1 cut(s) 1313
BspANI GGCC 3 cut(s) 335, 852, 1132
BspCNI CTCAG 5 cut(s) 59, 213, 361, 493, 613
BspFNI CGCG 3 cut(s) 963, 1042, 1313
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 2 cut(s) 1158, 1284
BspPI GGATC 1 cut(s) 599
BspT104I TTCGAA 1 cut(s) 347
BspTNI GGTCTC 1 cut(s) 1148
BsrI ACTGG 1 cut(s) 1059
BssECI CCNNGG 4 cut(s) 88, 228, 980, 1160
BssMI GATC 3 cut(s) 531, 604, 691
BssNI GRCGYC 1 cut(s) 1045
BssT1I CCWWGG 2 cut(s) 228, 980
Bst2UI CCWGG 2 cut(s) 358, 1161
Bst4CI ACNGT 6 cut(s) 89, 907, 1009, 1114, 1298, 1325
Bst6I CTCTTC 1 cut(s) 1151
BstACI GRCGYC 1 cut(s) 1045
BstBI TTCGAA 1 cut(s) 347
BstC8I GCNNGC 2 cut(s) 378, 507
BstDEI CTNAG 6 cut(s) 46, 200, 369, 501, 621, 1374
BstDSI CCRYGG 3 cut(s) 88, 228, 980
BstF5I GGATG 3 cut(s) 143, 445, 554
BstFNI CGCG 3 cut(s) 963, 1042, 1313
BstHHI GCGC 2 cut(s) 646, 965
BstKTI GATC 3 cut(s) 534, 607, 694
BstMAI GTCTC 1 cut(s) 1148
BstMBI GATC 3 cut(s) 531, 604, 691
BstMWI GCNNNNNNNGC 3 cut(s) 224, 932, 1193
BstNI CCWGG 2 cut(s) 358, 1161
BstNSI RCATGY 1 cut(s) 1001
BstSCI CCNGG 4 cut(s) 335, 356, 801, 1159
BstSFI CTRYAG 2 cut(s) 322, 1008
BstSLI GKGCMC 2 cut(s) 122, 576
BstUI CGCG 3 cut(s) 963, 1042, 1313
BstV1I GCAGC 5 cut(s) 367, 628, 935, 952, 1180
BsuRI GGCC 3 cut(s) 335, 852, 1132
BtgI CCRYGG 3 cut(s) 88, 228, 980
BtsCI GGATG 3 cut(s) 143, 445, 554
BtsI GCAGTG 2 cut(s) 782, 1036
BtsIMutI CAGTG 4 cut(s) 366, 782, 1036, 1303
BtuMI TCGCGA 1 cut(s) 1313
Cac8I GCNNGC 2 cut(s) 378, 507
CaiI CAGNNNCTG 1 cut(s) 1054
CciI TCATGA 1 cut(s) 694
CfoI GCGC 2 cut(s) 646, 965
Cfr13I GGNCC 1 cut(s) 334
CseI GACGC 3 cut(s) 969, 1034, 1110
Csp6I GTAC 2 cut(s) 656, 841
CviAII CATG 5 cut(s) 229, 695, 944, 981, 998
CviQI GTAC 2 cut(s) 656, 841
DdeI CTNAG 6 cut(s) 46, 200, 369, 501, 621, 1374
DpnI GATC 3 cut(s) 533, 606, 693
DpnII GATC 3 cut(s) 531, 604, 691
DraIII CACNNNGTG 1 cut(s) 1298
Eam1104I CTCTTC 1 cut(s) 1151
EarI CTCTTC 1 cut(s) 1151
EciI GGCGGA 2 cut(s) 166, 813
Ecl136II GAGCTC 2 cut(s) 712, 941
Eco130I CCWWGG 2 cut(s) 228, 980
Eco147I AGGCCT 1 cut(s) 1132
Eco24I GRGCYC 2 cut(s) 714, 943
Eco31I GGTCTC 1 cut(s) 1148
Eco53kI GAGCTC 2 cut(s) 712, 941
EcoICRI GAGCTC 2 cut(s) 712, 941
EcoRII CCWGG 2 cut(s) 356, 1159
EcoT14I CCWWGG 2 cut(s) 228, 980
EcoT38I GRGCYC 2 cut(s) 714, 943
ErhI CCWWGG 2 cut(s) 228, 980
FaeI CATG 5 cut(s) 232, 698, 947, 984, 1001
FaqI GGGAC 1 cut(s) 1008
FatI CATG 5 cut(s) 228, 694, 943, 980, 997
FauI CCCGC 3 cut(s) 223, 250, 461
FbaI TGATCA 1 cut(s) 691
Fnu4HI GCNGC 9 cut(s) 219, 222, 225, 381, 642, 924, 966, 1043, 1194
FokI GGATG 3 cut(s) 130, 432, 561
FriOI GRGCYC 2 cut(s) 714, 943
Fsp4HI GCNGC 9 cut(s) 219, 222, 225, 381, 642, 924, 966, 1043, 1194
FspBI CTAG 1 cut(s) 753
FspI TGCGCA 1 cut(s) 645
GlaI GCGC 2 cut(s) 645, 964
GluI GCNGC 9 cut(s) 219, 222, 225, 381, 642, 924, 966, 1043, 1194
GsaI CCCAGC 1 cut(s) 573
GsuI CTGGAG 2 cut(s) 420, 1076
HaeIII GGCC 3 cut(s) 335, 852, 1132
HapII CCGG 3 cut(s) 337, 803, 1337
HgaI GACGC 3 cut(s) 969, 1034, 1110
HhaI GCGC 2 cut(s) 646, 965
Hin1I GRCGYC 1 cut(s) 1045
Hin1II CATG 5 cut(s) 232, 698, 947, 984, 1001
Hin6I GCGC 2 cut(s) 644, 963
HinP1I GCGC 2 cut(s) 644, 963
HinfI GANTC 3 cut(s) 420, 698, 1346
HpaII CCGG 3 cut(s) 337, 803, 1337
Hpy166II GTNNAC 2 cut(s) 309, 397
Hpy188I TCNGA 2 cut(s) 1050, 1156
Hpy188III TCNNGA 6 cut(s) 413, 437, 695, 869, 1253, 1312
Hpy8I GTNNAC 2 cut(s) 309, 397
HpyAV CCTTC 3 cut(s) 397, 772, 924
HpyCH4III ACNGT 6 cut(s) 89, 907, 1009, 1114, 1298, 1325
HpyCH4V TGCA 4 cut(s) 923, 968, 1209, 1366
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 932, 1193
HpyF3I CTNAG 6 cut(s) 46, 200, 369, 501, 621, 1374
Hsp92I GRCGYC 1 cut(s) 1045
Hsp92II CATG 5 cut(s) 232, 698, 947, 984, 1001
HspAI GCGC 2 cut(s) 644, 963
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 3 cut(s) 531, 604, 691
LmnI GCTCC 3 cut(s) 938, 1019, 1282
Lsp1109I GCAGC 5 cut(s) 367, 628, 935, 952, 1180
LweI GCATC 3 cut(s) 152, 363, 1180
MaeI CTAG 1 cut(s) 753
MaeIII GTNAC 3 cut(s) 49, 860, 1298
MalI GATC 3 cut(s) 533, 606, 693
MboI GATC 3 cut(s) 531, 604, 691
MhlI GDGCHC 6 cut(s) 122, 576, 592, 714, 943, 1024
MluCI AATT 8 cut(s) 95, 462, 475, 535, 541, 636, 678, 795
MmeI TCCRAC 3 cut(s) 35, 773, 937
MseI TTAA 5 cut(s) 105, 540, 669, 831, 1037
MslI CAYNNNNRTG 3 cut(s) 371, 651, 1214
MspA1I CMGCKG 1 cut(s) 204
MspI CCGG 3 cut(s) 337, 803, 1337
MspR9I CCNGG 4 cut(s) 337, 358, 803, 1161
Mva1269I GAATGC 1 cut(s) 358
MvaI CCWGG 2 cut(s) 358, 1161
MvnI CGCG 3 cut(s) 963, 1042, 1313
MwoI GCNNNNNNNGC 3 cut(s) 224, 932, 1193
NciI CCSGG 2 cut(s) 337, 803
NcoI CCATGG 2 cut(s) 228, 980
NdeII GATC 3 cut(s) 531, 604, 691
NlaIII CATG 5 cut(s) 232, 698, 947, 984, 1001
NlaIV GGNNCC 2 cut(s) 1158, 1284
NmuCI GTSAC 2 cut(s) 860, 1298
NruI TCGCGA 1 cut(s) 1313
NsbI TGCGCA 1 cut(s) 645
NspI RCATGY 1 cut(s) 1001
NspV TTCGAA 1 cut(s) 347
PagI TCATGA 1 cut(s) 694
PceI AGGCCT 1 cut(s) 1132
PciI ACATGT 1 cut(s) 997
PctI GAATGC 1 cut(s) 358
PfeI GAWTC 3 cut(s) 420, 698, 1346
PflMI CCANNNNNTGG 1 cut(s) 1031
PkrI GCNGC 9 cut(s) 220, 223, 226, 382, 643, 925, 967, 1044, 1195
PscI ACATGT 1 cut(s) 997
Psp124BI GAGCTC 2 cut(s) 714, 943
Psp6I CCWGG 2 cut(s) 356, 1159
PspFI CCCAGC 1 cut(s) 569
PspGI CCWGG 2 cut(s) 356, 1159
PspN4I GGNNCC 2 cut(s) 1158, 1284
PspPI GGNCC 1 cut(s) 334
PstNI CAGNNNCTG 1 cut(s) 1054
RruI TCGCGA 1 cut(s) 1313
RsaI GTAC 2 cut(s) 657, 842
RsaNI GTAC 2 cut(s) 656, 841
RseI CAYNNNNRTG 3 cut(s) 371, 651, 1214
SacI GAGCTC 2 cut(s) 714, 943
SaqAI TTAA 5 cut(s) 105, 540, 669, 831, 1037
SatI GCNGC 9 cut(s) 219, 222, 225, 381, 642, 924, 966, 1043, 1194
Sau3AI GATC 3 cut(s) 531, 604, 691
Sau96I GGNCC 1 cut(s) 334
ScaI AGTACT 1 cut(s) 842
ScrFI CCNGG 4 cut(s) 337, 358, 803, 1161
SduI GDGCHC 6 cut(s) 122, 576, 592, 714, 943, 1024
SfaNI GCATC 3 cut(s) 152, 363, 1180
SfcI CTRYAG 2 cut(s) 322, 1008
SfuI TTCGAA 1 cut(s) 347
SmiMI CAYNNNNRTG 3 cut(s) 371, 651, 1214
SmlI CTYRAG 1 cut(s) 874
SmoI CTYRAG 1 cut(s) 874
Sse9I AATT 8 cut(s) 95, 462, 475, 535, 541, 636, 678, 795
SseBI AGGCCT 1 cut(s) 1132
SspI AATATT 1 cut(s) 431
SspMI CTAG 1 cut(s) 753
SstI GAGCTC 2 cut(s) 714, 943
StuI AGGCCT 1 cut(s) 1132
StyD4I CCNGG 4 cut(s) 335, 356, 801, 1159
StyI CCWWGG 2 cut(s) 228, 980
TaaI ACNGT 6 cut(s) 89, 907, 1009, 1114, 1298, 1325
TaqI TCGA 3 cut(s) 347, 676, 814
TasI AATT 8 cut(s) 95, 462, 475, 535, 541, 636, 678, 795
TatI WGTACW 1 cut(s) 840
TauI GCSGC 4 cut(s) 221, 224, 227, 1045
TfiI GAWTC 3 cut(s) 420, 698, 1346
Tru1I TTAA 5 cut(s) 105, 540, 669, 831, 1037
Tru9I TTAA 5 cut(s) 105, 540, 669, 831, 1037
TscAI CASTG 4 cut(s) 373, 789, 1036, 1303
TseFI GTSAC 2 cut(s) 860, 1298
TseI GCWGC 5 cut(s) 380, 641, 923, 965, 1193
Tsp45I GTSAC 2 cut(s) 860, 1298
TspDTI ATGAA 7 cut(s) 17, 50, 690, 751, 1232, 1248, 1359
TspGWI ACGGA 1 cut(s) 790
TspRI CASTG 4 cut(s) 373, 789, 1036, 1303
Van91I CCANNNNNTGG 1 cut(s) 1031
XceI RCATGY 1 cut(s) 1001
XcmI CCANNNNNNNNNTGG 1 cut(s) 1157
XspI CTAG 1 cut(s) 753
ZrmI AGTACT 1 cut(s) 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.