FvH4_7g01180

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008425
Physical Location & Seq
Reverse (-)
1588842 .. 1595243
6402 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01180.t1

Sequence Viewer

Length: 1143 bp
ATGGTTTCCGCTCGCAACTACACCCACGCCCACTATCTTCCTCCTTATTTCAGCGTGGCGCCTATGATGGAATGGACTGATGTTCACTACAGGACTATGGCTCGCCTCATCTCCAAGCACGCCTGGCTCTACACTGAGATGCTTGCCGCCGAGACTATTGTCTATCAGAAGGACAATCTGGACAGATTCTTGTACTTTCCTCCAGAACAACATCCTATTGTTCTACAAATAGGTGGCAGTAATTTAGAGAACCTGGCCAAAGCTACTCAACTTGCTAATATTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCCAGTCCAAGAGTAGCTGGACATGGGTGCTTTGGTGCTCGTCTTATGCTGGATCCAAAGTTTGTTGCCGAGGCTATGTCAGTGATTGCTGCGAACACAGATGTCCCTGTCAGTGTTAAGTGTCGAATTGGTGTTGATGATCACGACTCATATAATGAGCTCTGTGATTTTATTTATAAAGTTTCTTCTCAATCACCAACTAGGCACTTCATTATACATTCTCGGAAGGCACTACTGAATGGAATCAGCCCAGCTGAAAATCGAAGTATTCCACCTCTTAAATACGAGTACTTTTATGGCCTCTTGCGTGACTTTCCAGATCTGAGATTCACAATAAATGGTGGCATAAACACTATTGATGAGGTCAGTGCAGCTAGGAAAGCAGGAGCTCATGGGGTAATGGTTGGACGGGCTGCATTTAATAACCCATGGAATACTTTGGGACACGTCGATACTGCTATTTATGGTGCACCAAGCAGTGGTGTAACACGGCGCCAGATACTGAAAGAGTTTGAAGCTTATGGAGAGACTGTTTTGGGAAAATATGGACTAAAGCCCAATGTTCGAGATGTGGCAAAGCCTTTGCTTAGCCTTTTCTATGCTCAGCCTGGGAATAGCCTTTGGAAACGTAAAGCTGATGCTGCTTTCATGCATTGCAAGACTATGAAATCATTTTTTGAGGAAACACTTGTAGCAATTCCAGACTTTGTTTTGGATGCACCTGTCAGAGCGCCACCACTTGGTAGTAAGGACCCTTTTGCAAACATACATACGTTATTGCCTCCGCCATATGAGTTAAGGGAACAAGAGCTACAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

381

Amino Acids

42.7

Weight (kDa)

7.67

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 19 - 327 2.7e-59 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
AccB1I GGYRCC 2 cut(s) 58, 810
AccB7I CCANNNNNTGG 2 cut(s) 797, 1058
AccBSI CCGCTC 1 cut(s) 11
AciI CCGC 3 cut(s) 9, 147, 1103
AclWI GGATC 2 cut(s) 365, 378
AcoI YGGCCR 1 cut(s) 255
AcyI GRCGYC 2 cut(s) 59, 811
AfaI GTAC 2 cut(s) 194, 608
AfiI CCNNNNNNNGG 2 cut(s) 797, 1058
AflIII ACRYGT 1 cut(s) 763
AgsI TTSAA 1 cut(s) 833
AjiI CACGTC 1 cut(s) 766
AjnI CCWGG 3 cut(s) 122, 252, 925
AluBI AGCT 9 cut(s) 263, 335, 478, 572, 692, 707, 836, 953, 1129
AluI AGCT 9 cut(s) 263, 335, 478, 572, 692, 707, 836, 953, 1129
Alw21I GWGCWC 4 cut(s) 358, 480, 709, 790
Alw26I GTCTC 2 cut(s) 146, 839
Alw44I GTGCAC 1 cut(s) 786
AlwI GGATC 2 cut(s) 365, 378
AlwNI CAGNNNCTG 1 cut(s) 820
AoxI GGCC 2 cut(s) 255, 616
ApaLI GTGCAC 1 cut(s) 786
ApeKI GCWGC 4 cut(s) 407, 689, 731, 959
AspLEI GCGC 3 cut(s) 61, 813, 1051
AspS9I GGNCC 1 cut(s) 1069
AsuHPI GGTGA 1 cut(s) 504
AvaII GGWCC 1 cut(s) 1069
BaeGI GKGCMC 1 cut(s) 790
BalI TGGCCA 1 cut(s) 257
BamHI GGATCC 1 cut(s) 370
BanI GGYRCC 2 cut(s) 58, 810
BanII GRGCYC 2 cut(s) 480, 709
Bbv12I GWGCWC 4 cut(s) 358, 480, 709, 790
BbvI GCAGC 4 cut(s) 394, 701, 718, 946
BccI CCATC 1 cut(s) 61
BceAI ACGGC 1 cut(s) 824
BciT130I CCWGG 3 cut(s) 124, 254, 927
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 2 cut(s) 146, 839
BfaI CTAG 2 cut(s) 519, 693
BfmI CTRYAG 1 cut(s) 88
BfoI RGCGCY 3 cut(s) 62, 814, 1052
BglII AGATCT 1 cut(s) 637
BisI GCNGC 5 cut(s) 147, 408, 690, 732, 960
BlpI GCTNAGC 2 cut(s) 905, 921
BlsI GCNGC 5 cut(s) 148, 409, 691, 733, 961
BmcAI AGTACT 1 cut(s) 608
Bme1390I CCNGG 3 cut(s) 124, 254, 927
Bme18I GGWCC 1 cut(s) 1069
BmgBI CACGTC 1 cut(s) 766
BmgT120I GGNCC 1 cut(s) 1069
BmiI GGNNCC 4 cut(s) 60, 372, 812, 1071
BmrFI CCNGG 3 cut(s) 124, 254, 927
BmrI ACTGGG 1 cut(s) 315
BmsI GCATC 3 cut(s) 129, 946, 1024
BmuI ACTGGG 1 cut(s) 315
BoxI GACNNNNGTC 1 cut(s) 158
BpmI CTGGAG 1 cut(s) 186
Bpu1102I GCTNAGC 2 cut(s) 905, 921
BsaHI GRCGYC 2 cut(s) 59, 811
BsaJI CCNNGG 3 cut(s) 387, 746, 926
BsaXI ACNNNNNCTCC 2 cut(s) 834, 864
Bsc4I CCNNNNNNNGG 2 cut(s) 797, 1058
Bse1I ACTGG 1 cut(s) 321
Bse3DI GCAATG 1 cut(s) 970
BseBI CCWGG 3 cut(s) 124, 254, 927
BseDI CCNNGG 3 cut(s) 387, 746, 926
BseGI GGATG 3 cut(s) 211, 320, 1039
BseLI CCNNNNNNNGG 2 cut(s) 797, 1058
BseMI GCAATG 1 cut(s) 970
BseMII CTCAG 3 cut(s) 126, 632, 935
BseNI ACTGG 1 cut(s) 321
BseSI GKGCMC 1 cut(s) 790
BseXI GCAGC 4 cut(s) 394, 701, 718, 946
BseYI CCCAGC 1 cut(s) 568
BsgI GTGCAG 1 cut(s) 708
BshFI GGCC 2 cut(s) 257, 618
BshNI GGYRCC 2 cut(s) 58, 810
BsiHKAI GWGCWC 4 cut(s) 358, 480, 709, 790
BslFI GGGAC 3 cut(s) 303, 407, 774
BslI CCNNNNNNNGG 2 cut(s) 797, 1058
BsmAI GTCTC 2 cut(s) 146, 839
BsmFI GGGAC 3 cut(s) 303, 407, 774
BsnI GGCC 2 cut(s) 257, 618
Bsp1286I GDGCHC 4 cut(s) 358, 480, 709, 790
Bsp143I GATC 4 cut(s) 297, 370, 457, 637
Bsp1720I GCTNAGC 2 cut(s) 905, 921
Bsp19I CCATGG 1 cut(s) 746
BspACI CCGC 3 cut(s) 9, 147, 1103
BspANI GGCC 2 cut(s) 257, 618
BspCNI CTCAG 3 cut(s) 127, 633, 934
BspLI GGNNCC 4 cut(s) 60, 372, 812, 1071
BspPI GGATC 2 cut(s) 365, 378
BspT107I GGYRCC 2 cut(s) 58, 810
BsrBI CCGCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 970
BsrI ACTGG 1 cut(s) 321
BssECI CCNNGG 3 cut(s) 387, 746, 926
BssMI GATC 4 cut(s) 297, 370, 457, 637
BssNI GRCGYC 2 cut(s) 59, 811
BssT1I CCWWGG 1 cut(s) 746
Bst2UI CCWGG 3 cut(s) 124, 254, 927
Bst4CI ACNGT 1 cut(s) 850
BstACI GRCGYC 2 cut(s) 59, 811
BstC8I GCNNGC 4 cut(s) 13, 103, 120, 144
BstDEI CTNAG 5 cut(s) 135, 641, 905, 921, 1140
BstDSI CCRYGG 1 cut(s) 746
BstF5I GGATG 3 cut(s) 211, 320, 1039
BstH2I RGCGCY 3 cut(s) 62, 814, 1052
BstHHI GCGC 3 cut(s) 61, 813, 1051
BstKTI GATC 4 cut(s) 300, 373, 460, 640
BstMAI GTCTC 2 cut(s) 146, 839
BstMBI GATC 4 cut(s) 297, 370, 457, 637
BstMWI GCNNNNNNNGC 3 cut(s) 124, 698, 959
BstNI CCWGG 3 cut(s) 124, 254, 927
BstPAI GACNNNNGTC 1 cut(s) 158
BstSCI CCNGG 3 cut(s) 122, 252, 925
BstSFI CTRYAG 1 cut(s) 88
BstSLI GKGCMC 1 cut(s) 790
BstV1I GCAGC 4 cut(s) 394, 701, 718, 946
BstX2I RGATCY 2 cut(s) 370, 637
BstYI RGATCY 2 cut(s) 370, 637
BsuRI GGCC 2 cut(s) 257, 618
BtgI CCRYGG 1 cut(s) 746
BtrI CACGTC 1 cut(s) 766
BtsCI GGATG 3 cut(s) 211, 320, 1039
BtsI GCAGTG 1 cut(s) 802
BtsIMutI CAGTG 5 cut(s) 132, 405, 436, 691, 802
Cac8I GCNNGC 4 cut(s) 13, 103, 120, 144
CaiI CAGNNNCTG 1 cut(s) 820
CfoI GCGC 3 cut(s) 61, 813, 1051
Cfr13I GGNCC 1 cut(s) 1069
Csp6I GTAC 2 cut(s) 193, 607
CviAII CATG 4 cut(s) 341, 710, 747, 967
CviQI GTAC 2 cut(s) 193, 607
DdeI CTNAG 5 cut(s) 135, 641, 905, 921, 1140
DinI GGCGCC 2 cut(s) 60, 812
DpnI GATC 4 cut(s) 299, 372, 459, 639
DpnII GATC 4 cut(s) 297, 370, 457, 637
EaeI YGGCCR 1 cut(s) 255
EciI GGCGGA 1 cut(s) 1092
Ecl136II GAGCTC 2 cut(s) 478, 707
Eco130I CCWWGG 1 cut(s) 746
Eco24I GRGCYC 2 cut(s) 480, 709
Eco47I GGWCC 1 cut(s) 1069
Eco53kI GAGCTC 2 cut(s) 478, 707
EcoICRI GAGCTC 2 cut(s) 478, 707
EcoO109I RGGNCCY 1 cut(s) 1069
EcoRII CCWGG 3 cut(s) 122, 252, 925
EcoT14I CCWWGG 1 cut(s) 746
EcoT22I ATGCAT 1 cut(s) 972
EcoT38I GRGCYC 2 cut(s) 480, 709
EgeI GGCGCC 2 cut(s) 60, 812
EheI GGCGCC 2 cut(s) 60, 812
ErhI CCWWGG 1 cut(s) 746
FaeI CATG 4 cut(s) 344, 713, 750, 970
FaqI GGGAC 3 cut(s) 303, 407, 774
FatI CATG 4 cut(s) 340, 709, 746, 966
FauNDI CATATG 1 cut(s) 1108
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 5 cut(s) 147, 408, 690, 732, 960
FokI GGATG 3 cut(s) 198, 327, 1046
FriOI GRGCYC 2 cut(s) 480, 709
Fsp4HI GCNGC 5 cut(s) 147, 408, 690, 732, 960
FspBI CTAG 2 cut(s) 519, 693
GlaI GCGC 3 cut(s) 60, 812, 1050
GluI GCNGC 5 cut(s) 147, 408, 690, 732, 960
GsaI CCCAGC 1 cut(s) 572
GsuI CTGGAG 1 cut(s) 186
HaeII RGCGCY 3 cut(s) 62, 814, 1052
HaeIII GGCC 2 cut(s) 257, 618
HhaI GCGC 3 cut(s) 61, 813, 1051
Hin1I GRCGYC 2 cut(s) 59, 811
Hin1II CATG 4 cut(s) 344, 713, 750, 970
Hin6I GCGC 3 cut(s) 59, 811, 1049
HinP1I GCGC 3 cut(s) 59, 811, 1049
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 4 cut(s) 186, 464, 561, 645
HphI GGTGA 1 cut(s) 504
Hpy166II GTNNAC 2 cut(s) 85, 788
Hpy188I TCNGA 4 cut(s) 168, 543, 642, 1046
Hpy188III TCNNGA 6 cut(s) 179, 203, 461, 635, 884, 1019
Hpy8I GTNNAC 2 cut(s) 85, 788
Hpy99I CGWCG 1 cut(s) 770
HpyAV CCTTC 2 cut(s) 163, 538
HpyCH4III ACNGT 1 cut(s) 850
HpyCH4IV ACGT 3 cut(s) 765, 946, 1091
HpyCH4V TGCA 7 cut(s) 689, 734, 788, 970, 975, 1037, 1079
HpyF10VI GCNNNNNNNGC 3 cut(s) 124, 698, 959
HpyF3I CTNAG 5 cut(s) 135, 641, 905, 921, 1140
HpySE526I ACGT 3 cut(s) 765, 946, 1091
Hsp92I GRCGYC 2 cut(s) 59, 811
Hsp92II CATG 4 cut(s) 344, 713, 750, 970
HspAI GCGC 3 cut(s) 59, 811, 1049
KasI GGCGCC 2 cut(s) 58, 810
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 4 cut(s) 297, 370, 457, 637
LmnI GCTCC 1 cut(s) 704
Lsp1109I GCAGC 4 cut(s) 394, 701, 718, 946
LweI GCATC 3 cut(s) 129, 946, 1024
MaeI CTAG 2 cut(s) 519, 693
MaeII ACGT 3 cut(s) 765, 946, 1091
MaeIII GTNAC 2 cut(s) 626, 802
MalI GATC 4 cut(s) 299, 372, 459, 639
MbiI CCGCTC 1 cut(s) 11
MboI GATC 4 cut(s) 297, 370, 457, 637
MboII GAAGA 2 cut(s) 29, 495
MflI RGATCY 2 cut(s) 370, 637
MhlI GDGCHC 4 cut(s) 358, 480, 709, 790
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 5 cut(s) 241, 301, 307, 444, 1014
MluNI TGGCCA 1 cut(s) 257
Mly113I GGCGCC 2 cut(s) 59, 811
MlyI GAGTC 1 cut(s) 458
MmeI TCCRAC 1 cut(s) 703
MnlI CCTC 9 cut(s) 51, 116, 210, 382, 603, 629, 673, 991, 1110
Mox20I TGGCCA 1 cut(s) 257
Mph1103I ATGCAT 1 cut(s) 972
MscI TGGCCA 1 cut(s) 257
MseI TTAA 5 cut(s) 306, 435, 597, 738, 1115
MslI CAYNNNNRTG 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 572
MspR9I CCNGG 3 cut(s) 124, 254, 927
MvaI CCWGG 3 cut(s) 124, 254, 927
MwoI GCNNNNNNNGC 3 cut(s) 124, 698, 959
NarI GGCGCC 2 cut(s) 59, 811
NcoI CCATGG 1 cut(s) 746
NdeI CATATG 1 cut(s) 1108
NdeII GATC 4 cut(s) 297, 370, 457, 637
NlaIII CATG 4 cut(s) 344, 713, 750, 970
NlaIV GGNNCC 4 cut(s) 60, 372, 812, 1071
NmeAIII GCCGAG 2 cut(s) 175, 412
NmuCI GTSAC 1 cut(s) 626
NsiI ATGCAT 1 cut(s) 972
PfeI GAWTC 3 cut(s) 186, 561, 645
PflMI CCANNNNNTGG 2 cut(s) 797, 1058
PkrI GCNGC 5 cut(s) 148, 409, 691, 733, 961
PleI GAGTC 1 cut(s) 458
PluTI GGCGCC 2 cut(s) 62, 814
PpsI GAGTC 1 cut(s) 458
PpuMI RGGWCCY 1 cut(s) 1069
PshAI GACNNNNGTC 1 cut(s) 158
PsiI TTATAA 1 cut(s) 495
Psp124BI GAGCTC 2 cut(s) 480, 709
Psp5II RGGWCCY 1 cut(s) 1069
Psp6I CCWGG 3 cut(s) 122, 252, 925
PspFI CCCAGC 1 cut(s) 568
PspGI CCWGG 3 cut(s) 122, 252, 925
PspN4I GGNNCC 4 cut(s) 60, 372, 812, 1071
PspPI GGNCC 1 cut(s) 1069
PspPPI RGGWCCY 1 cut(s) 1069
PsrI GAACNNNNNNTAC 1 cut(s) 1113
PstNI CAGNNNCTG 1 cut(s) 820
PsuI RGATCY 2 cut(s) 370, 637
PvuII CAGCTG 1 cut(s) 572
RsaI GTAC 2 cut(s) 194, 608
RsaNI GTAC 2 cut(s) 193, 607
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 2 cut(s) 480, 709
SaqAI TTAA 5 cut(s) 306, 435, 597, 738, 1115
SatI GCNGC 5 cut(s) 147, 408, 690, 732, 960
Sau3AI GATC 4 cut(s) 297, 370, 457, 637
Sau96I GGNCC 1 cut(s) 1069
ScaI AGTACT 1 cut(s) 608
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 3 cut(s) 124, 254, 927
SduI GDGCHC 4 cut(s) 358, 480, 709, 790
SfaNI GCATC 3 cut(s) 129, 946, 1024
SfcI CTRYAG 1 cut(s) 88
SfoI GGCGCC 2 cut(s) 60, 812
SinI GGWCC 1 cut(s) 1069
SmiMI CAYNNNNRTG 1 cut(s) 137
Sse9I AATT 5 cut(s) 241, 301, 307, 444, 1014
SsiI CCGC 3 cut(s) 9, 147, 1103
SspDI GGCGCC 2 cut(s) 58, 810
SspI AATATT 1 cut(s) 280
SspMI CTAG 2 cut(s) 519, 693
SstI GAGCTC 2 cut(s) 480, 709
StyD4I CCNGG 3 cut(s) 122, 252, 925
StyI CCWWGG 1 cut(s) 746
TaaI ACNGT 1 cut(s) 850
TaiI ACGT 3 cut(s) 768, 949, 1094
TaqI TCGA 4 cut(s) 442, 580, 768, 883
TasI AATT 5 cut(s) 241, 301, 307, 444, 1014
TatI WGTACW 2 cut(s) 192, 606
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 3 cut(s) 186, 561, 645
Tru1I TTAA 5 cut(s) 306, 435, 597, 738, 1115
Tru9I TTAA 5 cut(s) 306, 435, 597, 738, 1115
TscAI CASTG 5 cut(s) 139, 405, 436, 691, 802
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 4 cut(s) 407, 689, 731, 959
Tsp45I GTSAC 1 cut(s) 626
TspDTI ATGAA 3 cut(s) 517, 955, 998
TspRI CASTG 5 cut(s) 139, 405, 436, 691, 802
Van91I CCANNNNNTGG 2 cut(s) 797, 1058
VneI GTGCAC 1 cut(s) 786
VpaK11BI GGWCC 1 cut(s) 1069
XspI CTAG 2 cut(s) 519, 693
ZrmI AGTACT 1 cut(s) 608
Zsp2I ATGCAT 1 cut(s) 972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.