Rh1BG016700

Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0008425
Physical Location & Seq
Reverse (-)
2526758 .. 2533180
6423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG016700.1

Sequence Viewer

Length: 1284 bp
ATGAAGCTTGCAACTTGTTCATATTCGTCGTCACCAGCCTCCGTTACTCCTTTTCTCCTTCAAACCCGTCCCAGATTCTCATTCAATATATTTCAGCCCTCTTCTTCTTCCTCAACCGCCGCCCACCGACCACGACCCGTCATGGTTTCCGCTCGCAACTACGCCCAGGCCCACTATCTTCCTCCTTGGTTCAGTGTGGCTCCCATGATGGAATGGACTGATGTTCACTACAGGACTATGGCCCGCCTCATATCCAAGCATGCCTGGCTCTACACTGAGATGCTCGCTGCCGAAACCATTGTCTATCAGAAGGACAATCTGGACAGATTCTTGTACTTTCCTCCGGAACAGCATCCTATTGTTCTACAAATTGGTGGCAGTAATTTGGAAAACCTGGCAAAAGCTACTCAACTTGCTAATATTTACAAATATGATGAGATCAATTTTAATTGTGGATGTCCCAGTCCAAGAGTAGCTGGACATGGGTGCTTTGGTGCTCGTCTTATGCTGGATCCAAAGTTTGTTGCTGAGGCTATGTCAGTGATTGCTGCGAACACTGATGTCCCTGTCAGTGTTAAGTGTCGAATTGGTGTTGATGATCATGATTCATATAATGAGCTCTGTGATTTTATTTATAAAGTTTCTTCTCAATCACCAACTAGGCACTTCATTATACATTCTCGGAAGGCCATGCTGAATGGAATCAGCCCAGCTGAAAATCGAAGTATTCCACCTCTTAAATACGAGTACTTTTATGGCCTCTTGCGTGACTTTCCGGACTTAAGATTTACCATAAATGGTGAAATAAACACTATTGATGAGGTCAATGCAGCTAGGAAAGCAGGAGCTCATGGTGTAATGGTTGGACGGGCTGCATTTAATAACTCATGGAATACTTTGGGACATGTTGATACTGCTATTTATGGTGCACCAAGCAGCGGTATAACACGGCGTCAGTTACTCAAGGAATTTGAAGCTTATGGAGAGACTGTTTTGGGAAAATATGGACAAAAGCCCAATGTTCGAGATGTGGCCAAGCCTTTGCTTAGCCTTTTCTATGCTCAGCCTGGGAACAGCCTTTGGAAACGCAAAGCTGATGCTGCTTTCATGCATTGCACGACTATGAAATCATTTTTTGAGGAAACACTTGTAGCAATTCCAGACTTTGTCTTGGATGCACCTGTTGGAGCGCCACCATCTGGTAGTAATGATCCTTTTGCAAACATACATACTTTGTTGCCTCCACCATACGAATCAAGGGAACAAGAGCTACAATATGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dus

Protein Analysis

427

Amino Acids

47.75

Weight (kDa)

8.07

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 66 - 373 1.9e-57 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 636
AccB7I CCANNNNNTGG 1 cut(s) 1199
AccBSI CCGCTC 1 cut(s) 152
AccIII TCCGGA 2 cut(s) 343, 775
AciI CCGC 5 cut(s) 117, 120, 150, 244, 939
AclWI GGATC 3 cut(s) 506, 519, 1205
AcoI YGGCCR 1 cut(s) 1032
AcsI RAATTY 1 cut(s) 968
AcyI GRCGYC 1 cut(s) 952
AfaI GTAC 2 cut(s) 335, 749
AfiI CCNNNNNNNGG 2 cut(s) 938, 1199
AflII CTTAAG 1 cut(s) 781
AflIII ACRYGT 1 cut(s) 904
AgsI TTSAA 3 cut(s) 62, 85, 974
AjnI CCWGG 4 cut(s) 165, 263, 393, 1066
Alw21I GWGCWC 4 cut(s) 499, 621, 850, 931
Alw26I GTCTC 1 cut(s) 980
Alw44I GTGCAC 1 cut(s) 927
AlwI GGATC 3 cut(s) 506, 519, 1205
Aor13HI TCCGGA 2 cut(s) 343, 775
AoxI GGCC 5 cut(s) 168, 240, 687, 757, 1032
ApaLI GTGCAC 1 cut(s) 927
ApeKI GCWGC 6 cut(s) 287, 548, 830, 872, 936, 1100
ApoI RAATTY 1 cut(s) 968
ArsI GACNNNNNNTTYG 2 cut(s) 285, 317
AspLEI GCGC 1 cut(s) 1192
AspS9I GGNCC 2 cut(s) 169, 241
AsuHPI GGTGA 3 cut(s) 24, 645, 812
BaeGI GKGCMC 1 cut(s) 931
BalI TGGCCA 1 cut(s) 1034
BamHI GGATCC 1 cut(s) 511
BanII GRGCYC 2 cut(s) 621, 850
Bbv12I GWGCWC 4 cut(s) 499, 621, 850, 931
BbvCI CCTCAGC 1 cut(s) 528
BbvI GCAGC 6 cut(s) 274, 535, 842, 859, 948, 1087
BccI CCATC 2 cut(s) 202, 1204
BceAI ACGGC 1 cut(s) 965
BciT130I CCWGG 4 cut(s) 167, 265, 395, 1068
BclI TGATCA 1 cut(s) 598
BcoDI GTCTC 1 cut(s) 980
BfaI CTAG 2 cut(s) 660, 834
BfmI CTRYAG 1 cut(s) 229
BfoI RGCGCY 1 cut(s) 1193
BfrI CTTAAG 1 cut(s) 781
BisI GCNGC 7 cut(s) 120, 288, 549, 831, 873, 937, 1101
BlpI GCTNAGC 2 cut(s) 1046, 1062
BlsI GCNGC 7 cut(s) 121, 289, 550, 832, 874, 938, 1102
BmcAI AGTACT 1 cut(s) 749
Bme1390I CCNGG 4 cut(s) 167, 265, 395, 1068
BmgT120I GGNCC 2 cut(s) 169, 241
BmiI GGNNCC 2 cut(s) 201, 513
BmrFI CCNGG 4 cut(s) 167, 265, 395, 1068
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 4 cut(s) 270, 361, 1087, 1165
BmuI ACTGGG 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 528
Bpu1102I GCTNAGC 2 cut(s) 1046, 1062
BpuEI CTTGAG 1 cut(s) 947
BsaHI GRCGYC 1 cut(s) 952
BsaJI CCNNGG 3 cut(s) 165, 185, 1067
BsaWI WCCGGW 2 cut(s) 343, 775
BsaXI ACNNNNNCTCC 2 cut(s) 975, 1005
Bsc4I CCNNNNNNNGG 2 cut(s) 938, 1199
Bse1I ACTGG 1 cut(s) 462
Bse3DI GCAATG 1 cut(s) 1111
BseAI TCCGGA 2 cut(s) 343, 775
BseBI CCWGG 4 cut(s) 167, 265, 395, 1068
BseDI CCNNGG 3 cut(s) 165, 185, 1067
BseGI GGATG 3 cut(s) 352, 461, 1180
BseLI CCNNNNNNNGG 2 cut(s) 938, 1199
BseMI GCAATG 1 cut(s) 1111
BseMII CTCAG 3 cut(s) 267, 519, 1076
BseNI ACTGG 1 cut(s) 462
BseSI GKGCMC 1 cut(s) 931
BseXI GCAGC 6 cut(s) 274, 535, 842, 859, 948, 1087
BseYI CCCAGC 1 cut(s) 709
BshFI GGCC 5 cut(s) 170, 242, 689, 759, 1034
BsiHKAI GWGCWC 4 cut(s) 499, 621, 850, 931
BsiSI CCGG 2 cut(s) 344, 776
BslFI GGGAC 4 cut(s) 54, 444, 548, 915
BslI CCNNNNNNNGG 2 cut(s) 938, 1199
BsmAI GTCTC 1 cut(s) 980
BsmFI GGGAC 4 cut(s) 54, 444, 548, 915
BsnI GGCC 5 cut(s) 170, 242, 689, 759, 1034
Bsp1286I GDGCHC 4 cut(s) 499, 621, 850, 931
Bsp13I TCCGGA 2 cut(s) 343, 775
Bsp143I GATC 4 cut(s) 438, 511, 598, 1210
Bsp1720I GCTNAGC 2 cut(s) 1046, 1062
BspACI CCGC 5 cut(s) 117, 120, 150, 244, 939
BspANI GGCC 5 cut(s) 170, 242, 689, 759, 1034
BspCNI CTCAG 3 cut(s) 268, 520, 1075
BspEI TCCGGA 2 cut(s) 343, 775
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 2 cut(s) 201, 513
BspPI GGATC 3 cut(s) 506, 519, 1205
BspTI CTTAAG 1 cut(s) 781
BsrBI CCGCTC 1 cut(s) 152
BsrDI GCAATG 1 cut(s) 1111
BsrI ACTGG 1 cut(s) 462
BssECI CCNNGG 3 cut(s) 165, 185, 1067
BssMI GATC 4 cut(s) 438, 511, 598, 1210
BssNI GRCGYC 1 cut(s) 952
BssT1I CCWWGG 1 cut(s) 185
Bst2UI CCWGG 4 cut(s) 167, 265, 395, 1068
Bst4CI ACNGT 1 cut(s) 991
Bst6I CTCTTC 1 cut(s) 106
BstACI GRCGYC 1 cut(s) 952
BstAFI CTTAAG 1 cut(s) 781
BstC8I GCNNGC 5 cut(s) 9, 154, 244, 261, 285
BstDEI CTNAG 5 cut(s) 276, 528, 1046, 1062, 1281
BstF5I GGATG 3 cut(s) 352, 461, 1180
BstH2I RGCGCY 1 cut(s) 1193
BstHHI GCGC 1 cut(s) 1192
BstKTI GATC 4 cut(s) 441, 514, 601, 1213
BstMAI GTCTC 1 cut(s) 980
BstMBI GATC 4 cut(s) 438, 511, 598, 1210
BstMWI GCNNNNNNNGC 3 cut(s) 265, 839, 1100
BstNI CCWGG 4 cut(s) 167, 265, 395, 1068
BstNSI RCATGY 2 cut(s) 263, 908
BstSCI CCNGG 4 cut(s) 165, 263, 393, 1066
BstSFI CTRYAG 1 cut(s) 229
BstSLI GKGCMC 1 cut(s) 931
BstV1I GCAGC 6 cut(s) 274, 535, 842, 859, 948, 1087
BstX2I RGATCY 1 cut(s) 511
BstYI RGATCY 1 cut(s) 511
BsuRI GGCC 5 cut(s) 170, 242, 689, 759, 1034
BtsCI GGATG 3 cut(s) 352, 461, 1180
BtsIMutI CAGTG 5 cut(s) 199, 273, 546, 555, 577
Cac8I GCNNGC 5 cut(s) 9, 154, 244, 261, 285
CciI TCATGA 1 cut(s) 601
CfoI GCGC 1 cut(s) 1192
Cfr13I GGNCC 2 cut(s) 169, 241
CseI GACGC 1 cut(s) 941
Csp6I GTAC 2 cut(s) 334, 748
CviQI GTAC 2 cut(s) 334, 748
DdeI CTNAG 5 cut(s) 276, 528, 1046, 1062, 1281
DpnI GATC 4 cut(s) 440, 513, 600, 1212
DpnII GATC 4 cut(s) 438, 511, 598, 1210
EaeI YGGCCR 1 cut(s) 1032
Eam1104I CTCTTC 1 cut(s) 106
EarI CTCTTC 1 cut(s) 106
Ecl136II GAGCTC 2 cut(s) 619, 848
Eco130I CCWWGG 1 cut(s) 185
Eco24I GRGCYC 2 cut(s) 621, 850
Eco53kI GAGCTC 2 cut(s) 619, 848
EcoICRI GAGCTC 2 cut(s) 619, 848
EcoRII CCWGG 4 cut(s) 165, 263, 393, 1066
EcoT14I CCWWGG 1 cut(s) 185
EcoT22I ATGCAT 1 cut(s) 1113
EcoT38I GRGCYC 2 cut(s) 621, 850
ErhI CCWWGG 1 cut(s) 185
FaqI GGGAC 4 cut(s) 54, 444, 548, 915
FauI CCCGC 1 cut(s) 251
FbaI TGATCA 1 cut(s) 598
Fnu4HI GCNGC 7 cut(s) 120, 288, 549, 831, 873, 937, 1101
FokI GGATG 3 cut(s) 339, 468, 1187
FriOI GRGCYC 2 cut(s) 621, 850
Fsp4HI GCNGC 7 cut(s) 120, 288, 549, 831, 873, 937, 1101
FspBI CTAG 2 cut(s) 660, 834
GlaI GCGC 1 cut(s) 1191
GluI GCNGC 7 cut(s) 120, 288, 549, 831, 873, 937, 1101
GsaI CCCAGC 1 cut(s) 713
HaeII RGCGCY 1 cut(s) 1193
HaeIII GGCC 5 cut(s) 170, 242, 689, 759, 1034
HapII CCGG 2 cut(s) 344, 776
HgaI GACGC 1 cut(s) 941
HhaI GCGC 1 cut(s) 1192
Hin1I GRCGYC 1 cut(s) 952
Hin6I GCGC 1 cut(s) 1190
HinP1I GCGC 1 cut(s) 1190
HindIII AAGCTT 2 cut(s) 5, 975
HinfI GANTC 5 cut(s) 75, 327, 605, 702, 1253
HpaII CCGG 2 cut(s) 344, 776
HphI GGTGA 3 cut(s) 24, 645, 812
Hpy166II GTNNAC 2 cut(s) 226, 929
Hpy188I TCNGA 2 cut(s) 309, 684
Hpy188III TCNNGA 6 cut(s) 320, 344, 602, 776, 1025, 1160
Hpy8I GTNNAC 2 cut(s) 226, 929
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 3 cut(s) 68, 304, 679
HpyCH4III ACNGT 1 cut(s) 991
HpyCH4V TGCA 8 cut(s) 11, 830, 875, 929, 1111, 1116, 1178, 1220
HpyF10VI GCNNNNNNNGC 3 cut(s) 265, 839, 1100
HpyF3I CTNAG 5 cut(s) 276, 528, 1046, 1062, 1281
Hsp92I GRCGYC 1 cut(s) 952
HspAI GCGC 1 cut(s) 1190
Kpn2I TCCGGA 2 cut(s) 343, 775
Ksp22I TGATCA 1 cut(s) 598
Kzo9I GATC 4 cut(s) 438, 511, 598, 1210
LmnI GCTCC 3 cut(s) 205, 845, 1187
Lsp1109I GCAGC 6 cut(s) 274, 535, 842, 859, 948, 1087
LweI GCATC 4 cut(s) 270, 361, 1087, 1165
MaeI CTAG 2 cut(s) 660, 834
MaeIII GTNAC 4 cut(s) 30, 43, 767, 957
MalI GATC 4 cut(s) 440, 513, 600, 1212
MbiI CCGCTC 1 cut(s) 152
MboI GATC 4 cut(s) 438, 511, 598, 1210
MboII GAAGA 5 cut(s) 93, 96, 99, 170, 636
MflI RGATCY 1 cut(s) 511
MhlI GDGCHC 4 cut(s) 499, 621, 850, 931
MlsI TGGCCA 1 cut(s) 1034
MluCI AATT 7 cut(s) 369, 382, 442, 448, 585, 968, 1155
MluNI TGGCCA 1 cut(s) 1034
MmeI TCCRAC 2 cut(s) 844, 1165
Mox20I TGGCCA 1 cut(s) 1034
Mph1103I ATGCAT 1 cut(s) 1113
MroI TCCGGA 2 cut(s) 343, 775
MscI TGGCCA 1 cut(s) 1034
MseI TTAA 5 cut(s) 447, 576, 738, 782, 879
MslI CAYNNNNRTG 2 cut(s) 278, 1121
Msp20I TGGCCA 1 cut(s) 1034
MspA1I CMGCKG 2 cut(s) 713, 939
MspCI CTTAAG 1 cut(s) 781
MspI CCGG 2 cut(s) 344, 776
MspR9I CCNGG 4 cut(s) 167, 265, 395, 1068
MvaI CCWGG 4 cut(s) 167, 265, 395, 1068
MwoI GCNNNNNNNGC 3 cut(s) 265, 839, 1100
NdeII GATC 4 cut(s) 438, 511, 598, 1210
NlaIV GGNNCC 2 cut(s) 201, 513
NmuCI GTSAC 2 cut(s) 30, 767
NsiI ATGCAT 1 cut(s) 1113
NspI RCATGY 2 cut(s) 263, 908
PaeI GCATGC 1 cut(s) 263
PagI TCATGA 1 cut(s) 601
PciI ACATGT 1 cut(s) 904
PfeI GAWTC 5 cut(s) 75, 327, 605, 702, 1253
PflFI GACNNNGTC 1 cut(s) 1166
PflMI CCANNNNNTGG 1 cut(s) 1199
PkrI GCNGC 7 cut(s) 121, 289, 550, 832, 874, 938, 1102
PscI ACATGT 1 cut(s) 904
PsiI TTATAA 1 cut(s) 636
Psp124BI GAGCTC 2 cut(s) 621, 850
Psp6I CCWGG 4 cut(s) 165, 263, 393, 1066
PspFI CCCAGC 1 cut(s) 709
PspGI CCWGG 4 cut(s) 165, 263, 393, 1066
PspN4I GGNNCC 2 cut(s) 201, 513
PspPI GGNCC 2 cut(s) 169, 241
PsrI GAACNNNNNNTAC 1 cut(s) 1254
PsuI RGATCY 1 cut(s) 511
PsyI GACNNNGTC 1 cut(s) 1166
PvuII CAGCTG 1 cut(s) 713
RsaI GTAC 2 cut(s) 335, 749
RsaNI GTAC 2 cut(s) 334, 748
RseI CAYNNNNRTG 2 cut(s) 278, 1121
SacI GAGCTC 2 cut(s) 621, 850
SaqAI TTAA 5 cut(s) 447, 576, 738, 782, 879
SatI GCNGC 7 cut(s) 120, 288, 549, 831, 873, 937, 1101
Sau3AI GATC 4 cut(s) 438, 511, 598, 1210
Sau96I GGNCC 2 cut(s) 169, 241
ScaI AGTACT 1 cut(s) 749
ScrFI CCNGG 4 cut(s) 167, 265, 395, 1068
SduI GDGCHC 4 cut(s) 499, 621, 850, 931
SfaNI GCATC 4 cut(s) 270, 361, 1087, 1165
SfcI CTRYAG 1 cut(s) 229
SmiMI CAYNNNNRTG 2 cut(s) 278, 1121
SmlI CTYRAG 2 cut(s) 781, 962
SmoI CTYRAG 2 cut(s) 781, 962
SphI GCATGC 1 cut(s) 263
Sse9I AATT 7 cut(s) 369, 382, 442, 448, 585, 968, 1155
SsiI CCGC 5 cut(s) 117, 120, 150, 244, 939
SspI AATATT 1 cut(s) 421
SspMI CTAG 2 cut(s) 660, 834
SstI GAGCTC 2 cut(s) 621, 850
StyD4I CCNGG 4 cut(s) 165, 263, 393, 1066
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 1 cut(s) 991
TaqI TCGA 3 cut(s) 583, 721, 1024
TasI AATT 7 cut(s) 369, 382, 442, 448, 585, 968, 1155
TatI WGTACW 2 cut(s) 333, 747
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 5 cut(s) 75, 327, 605, 702, 1253
Tru1I TTAA 5 cut(s) 447, 576, 738, 782, 879
Tru9I TTAA 5 cut(s) 447, 576, 738, 782, 879
TscAI CASTG 5 cut(s) 199, 280, 546, 562, 577
TseFI GTSAC 2 cut(s) 30, 767
TseI GCWGC 6 cut(s) 287, 548, 830, 872, 936, 1100
Tsp45I GTSAC 2 cut(s) 30, 767
TspDTI ATGAA 6 cut(s) 9, 17, 597, 658, 1096, 1139
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 5 cut(s) 199, 280, 546, 562, 577
Tth111I GACNNNGTC 1 cut(s) 1166
Van91I CCANNNNNTGG 1 cut(s) 1199
Vha464I CTTAAG 1 cut(s) 781
VneI GTGCAC 1 cut(s) 927
XapI RAATTY 1 cut(s) 968
XceI RCATGY 2 cut(s) 263, 908
XspI CTAG 2 cut(s) 660, 834
ZrmI AGTACT 1 cut(s) 749
Zsp2I ATGCAT 1 cut(s) 1113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.